##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939736_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1770947 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.289837584072252 31.0 30.0 33.0 26.0 34.0 2 30.342404374608613 31.0 30.0 33.0 26.0 34.0 3 30.288711632815662 31.0 30.0 34.0 26.0 34.0 4 33.97122556462729 35.0 35.0 37.0 28.0 37.0 5 33.84836248628559 35.0 35.0 37.0 28.0 37.0 6 33.86663180772773 35.0 35.0 37.0 28.0 37.0 7 33.843598368556485 35.0 35.0 37.0 28.0 37.0 8 33.683311810008995 35.0 33.0 37.0 28.0 37.0 9 35.01117142410247 37.0 35.0 39.0 28.0 39.0 10-11 34.80249211297684 37.0 34.0 39.0 27.0 39.0 12-13 34.68829501955733 37.0 34.0 39.0 27.0 39.0 14-15 35.276276760399945 38.0 34.0 40.0 27.0 41.0 16-17 35.08688487007234 38.0 33.0 40.0 26.5 41.0 18-19 35.14288682834665 38.0 33.5 40.0 26.5 41.0 20-21 35.244939571878774 38.0 34.0 40.0 26.5 41.0 22-23 35.15947992797074 38.0 34.0 40.0 26.0 41.0 24-25 34.93120770977336 38.0 34.0 40.0 25.0 41.0 26-27 34.80885085776141 38.0 33.5 40.0 24.5 41.0 28-29 34.66126146067613 38.0 33.0 40.0 24.0 41.0 30-31 34.41345223770107 38.0 33.0 40.0 24.0 41.0 32-33 34.043478150390726 38.0 33.0 40.0 23.0 41.0 34-35 33.73598165275415 37.5 32.5 40.0 21.5 41.0 36-37 33.453368734355124 37.0 32.0 40.0 19.5 41.0 38-39 33.114838558127374 37.0 31.0 40.0 18.0 41.0 40-41 32.76477500455971 37.0 31.0 40.0 16.0 41.0 42-43 32.363493656218964 37.0 30.5 40.0 14.5 41.0 44-45 31.88836989475123 36.0 30.0 39.0 9.0 41.0 46-47 31.381413164820856 36.0 29.5 39.0 8.0 40.0 48-49 30.93933048250456 35.5 29.0 39.0 6.0 40.0 50-51 30.125568128238733 34.5 27.5 38.5 2.0 39.5 52-53 30.1862000952033 35.0 28.0 38.5 2.0 40.0 54-55 30.229063320359106 35.0 28.0 39.0 2.0 40.0 56-57 29.958253691386584 35.0 28.0 38.5 2.0 40.0 58-59 29.391515386965278 34.0 26.5 38.0 2.0 40.0 60-61 28.875471428563362 34.0 26.0 38.0 2.0 40.0 62-63 28.404916126795438 34.0 26.0 37.5 2.0 39.5 64-65 27.837473397001716 33.0 25.0 37.0 2.0 39.0 66-67 27.284837716769616 33.0 23.0 36.0 2.0 39.0 68-69 26.73425969269549 32.0 22.0 36.0 2.0 38.5 70-71 26.156377350649116 32.0 21.0 35.0 2.0 38.0 72-73 25.674636508037793 31.5 19.5 35.0 2.0 37.5 74-75 25.092864721530347 31.0 18.0 35.0 2.0 37.0 76-77 24.466893701505466 31.0 13.0 34.5 2.0 36.0 78-79 23.825373656015678 30.0 7.5 34.0 2.0 36.0 80-81 23.171923552765836 30.0 4.0 34.0 2.0 35.0 82-83 22.439250581750894 29.0 2.0 34.0 2.0 35.0 84-85 21.717723060035112 29.0 2.0 34.0 2.0 35.0 86-87 20.94784287728543 28.0 2.0 33.5 2.0 35.0 88-89 20.080256495536005 26.5 2.0 33.0 2.0 34.5 90-91 19.147218126798826 25.5 2.0 32.5 2.0 34.0 92-93 18.18442618553802 24.5 2.0 32.0 2.0 34.0 94-95 16.833224540316564 18.5 2.0 31.5 2.0 34.0 96-97 14.36079199433975 2.0 2.0 30.5 2.0 34.0 98-99 13.036022534835881 2.0 2.0 29.5 2.0 34.0 100-101 11.784956297393428 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.744264729663378 1101 2 0.7369817171192317 1101 3 0.7838676673646567 1101 4 0.8514054219175407 1101 5 0.8099739179510195 1101 6 1.0250267665677413 1101 7 1.0154334634553024 1101 8 0.6548089032172157 1101 9 0.7645321885987002 1101 10-11 0.8034740383807915 1101 12-13 0.6483611202991213 1101 14-15 0.8698185491185058 1101 16-17 0.874436387537898 1101 18-19 0.6337502711094061 1101 20-21 0.796604140785206 1101 22-23 0.6975677134603089 1101 24-25 0.8695800020384823 1101 26-27 0.6952154936588073 1101 28-29 0.922859987707362 1101 30-31 1.1076573829850815 1101 32-33 1.1185653491558654 1101 34-35 0.9148588067672776 1101 36-37 0.9976031035484993 1101 38-39 0.9282411963826434 1101 40-41 1.1338474198862514 1101 42-43 0.817183965039149 1101 44-45 1.0726448737959764 1101 46-47 0.9270202661633746 1101 48-49 0.916158318374837 1101 50-51 1.086477391576011 1101 52-53 1.0823755903075742 1101 54-55 0.9661565310007703 1101 56-57 0.7140702243814552 1101 58-59 0.6291029521238336 1101 60-61 0.8435013583636959 1101 62-63 0.5066791759311862 1101 64-65 0.5973704395959487 1101 66-67 0.3343522186719845 1101 68-69 0.4306583677768181 1101 70-71 0.31225523866426386 1101 72-73 0.2151148053439016 1101 74-75 0.2411054333393814 1101 76-77 -0.11676546009361743 1101 78-79 -0.256260899182049 1101 80-81 -0.2928175008512568 1101 82-83 -0.21346093703035152 1101 84-85 -0.301227938968438 1101 86-87 -0.18438008279153095 1101 88-89 -0.38437832221850243 1101 90-91 -0.3990552126012368 1101 92-93 -0.30699492231090986 1101 94-95 0.027244169385244987 1101 96-97 1.445947814127896 1101 98-99 1.391498670106806 1101 100-101 0.7023707122764549 1102 1 0.6054956008333185 1102 2 0.7453263247032815 1102 3 0.7496809258835029 1102 4 0.735700584571461 1102 5 0.9054831650526793 1102 6 0.9529169913768598 1102 7 0.8003307320395336 1102 8 0.8603869703397322 1102 9 1.1233198437650742 1102 10-11 0.9472567461732666 1102 12-13 0.7569443431060137 1102 14-15 0.8991316009889232 1102 16-17 0.9735888519727567 1102 18-19 0.9564783295338444 1102 20-21 0.8435338326740691 1102 22-23 0.7806093567197436 1102 24-25 0.8691744614229293 1102 26-27 1.1236965778483778 1102 28-29 1.2276434279106212 1102 30-31 1.2424564243136587 1102 32-33 1.1752019770080864 1102 34-35 1.027825690941107 1102 36-37 1.0781547163480738 1102 38-39 0.9957221575803814 1102 40-41 1.003186056272547 1102 42-43 0.7355883067614144 1102 44-45 0.768356725000249 1102 46-47 0.8764271353122552 1102 48-49 0.7290268090878449 1102 50-51 0.8825926781303437 1102 52-53 0.7761170792178085 1102 54-55 0.6862830906977884 1102 56-57 0.7107871182505896 1102 58-59 0.586749442590861 1102 60-61 0.8033932806816182 1102 62-63 0.6319804228023713 1102 64-65 0.24673043888680724 1102 66-67 0.338277153768864 1102 68-69 0.2804451819450371 1102 70-71 0.3643697008409532 1102 72-73 0.10593490597559452 1102 74-75 0.06629831443952838 1102 76-77 0.015716127143598158 1102 78-79 -0.4454888096838907 1102 80-81 -0.3985721886680871 1102 82-83 -0.3204460971247123 1102 84-85 -0.40261226183195475 1102 86-87 -0.5259510983826843 1102 88-89 -0.26707930028053184 1102 90-91 -0.6102166654117092 1102 92-93 -0.5920417755484202 1102 94-95 -0.49935447504538644 1102 96-97 0.6906414370571188 1102 98-99 0.3320601000917698 1102 100-101 0.03551595675872221 1103 1 0.6721755499173874 1103 2 0.6880005180731423 1103 3 0.745440514128564 1103 4 0.7327026322774799 1103 5 0.7872524937371637 1103 6 0.6757760317222008 1103 7 0.7298741489414127 1103 8 0.4761271883927449 1103 9 0.7422727465267158 1103 10-11 0.5629410673863902 1103 12-13 0.6578414597145752 1103 14-15 0.7018589240283859 1103 16-17 0.8593279631576607 1103 18-19 0.7648894203523753 1103 20-21 0.6387778970937887 1103 22-23 0.5699217149022715 1103 24-25 0.7185875446040626 1103 26-27 1.002707867903169 1103 28-29 0.9481752780406794 1103 30-31 1.003022587355332 1103 32-33 0.9874397876229537 1103 34-35 1.2203490735300804 1103 36-37 0.9356844634287071 1103 38-39 0.8249119301189864 1103 40-41 0.6788427057827633 1103 42-43 0.7255537171327688 1103 44-45 0.8981038056355892 1103 46-47 0.7394602306689464 1103 48-49 0.6311782839896125 1103 50-51 0.774822463951363 1103 52-53 0.8554009355463315 1103 54-55 0.677914614301752 1103 56-57 0.7824269433654258 1103 58-59 0.3844828534049576 1103 60-61 0.6147419994817724 1103 62-63 0.4816325894967086 1103 64-65 0.4119974708199514 1103 66-67 0.26171148881784134 1103 68-69 0.053235873184174665 1103 70-71 0.22715568789059404 1103 72-73 0.07402002413376252 1103 74-75 -0.06994276143628042 1103 76-77 -0.20545493932382897 1103 78-79 -0.17413234280691725 1103 80-81 -0.13232945249010086 1103 82-83 -0.05227298295126914 1103 84-85 -0.1489471447806494 1103 86-87 -0.37087168059531095 1103 88-89 -0.41685128544849803 1103 90-91 -0.44079970819216285 1103 92-93 -0.5891420577997906 1103 94-95 -0.19751652053766833 1103 96-97 1.0877655169729952 1103 98-99 1.1724059963782274 1103 100-101 0.6124081478041585 1104 1 0.44157644870705326 1104 2 0.3313486474326872 1104 3 0.5491564304253274 1104 4 0.6652115236783231 1104 5 0.6117345868694173 1104 6 0.9361476784923468 1104 7 0.7663004950581467 1104 8 0.7890551473682379 1104 9 0.8110952217824234 1104 10-11 0.8411536890400555 1104 12-13 0.633075393160027 1104 14-15 0.8919882284313516 1104 16-17 1.0643876527168104 1104 18-19 1.0416361326018304 1104 20-21 0.9033174741663856 1104 22-23 0.7934814458167736 1104 24-25 0.8988512539035014 1104 26-27 1.0735786737811424 1104 28-29 0.9145020956084338 1104 30-31 0.868561723304154 1104 32-33 1.0330430243685598 1104 34-35 1.0335718961707485 1104 36-37 0.8275927372562748 1104 38-39 0.6168002155260552 1104 40-41 0.7274570257629165 1104 42-43 0.7985689359025798 1104 44-45 0.8505630706131235 1104 46-47 0.5537869655259939 1104 48-49 0.34149024120060645 1104 50-51 0.5847518841493198 1104 52-53 0.6798689678586456 1104 54-55 0.5131036082638687 1104 56-57 0.3525341915802578 1104 58-59 0.5254989544223001 1104 60-61 0.6563430030383302 1104 62-63 0.28392546810496455 1104 64-65 0.4300590075848625 1104 66-67 0.18108054398588536 1104 68-69 0.21559023341686512 1104 70-71 0.28355418806980026 1104 72-73 0.31585316907048977 1104 74-75 0.3250741942583524 1104 76-77 0.07322665203014367 1104 78-79 -0.18122506150780993 1104 80-81 -0.11269696372497151 1104 82-83 -0.156538925439758 1104 84-85 -0.19026183144492492 1104 86-87 -0.4805231928227123 1104 88-89 -0.30454200255030983 1104 90-91 -0.3025282240864051 1104 92-93 -0.05617646545328725 1104 94-95 -0.06678370368673825 1104 96-97 1.2128388537985586 1104 98-99 1.5236262549870343 1104 100-101 1.3725599219487918 1105 1 0.5878002734910268 1105 2 0.7274266569497456 1105 3 0.7439334376137445 1105 4 0.6163182890665055 1105 5 0.8186335198318133 1105 6 0.7604631170826579 1105 7 0.8028871962656012 1105 8 0.7848058703918639 1105 9 0.9043017160092433 1105 10-11 1.0074982323576478 1105 12-13 0.5824671784231086 1105 14-15 0.8315465622415701 1105 16-17 0.924794570853372 1105 18-19 0.9289147410069489 1105 20-21 0.8808428020031229 1105 22-23 0.6355497555922298 1105 24-25 0.7182370359795556 1105 26-27 1.1031495972264764 1105 28-29 0.9046337035161329 1105 30-31 0.9044439716985408 1105 32-33 0.8184520609533337 1105 34-35 0.9179021473911959 1105 36-37 0.8981092754587792 1105 38-39 0.8591882459084559 1105 40-41 0.7483291056058547 1105 42-43 0.8267467733026024 1105 44-45 0.6204998693242239 1105 46-47 0.7013941094836298 1105 48-49 0.4897475011501591 1105 50-51 0.6474369044644099 1105 52-53 0.8902770965370408 1105 54-55 0.8500961711350108 1105 56-57 0.5943923347851552 1105 58-59 0.4879083288582997 1105 60-61 0.7786755465848216 1105 62-63 0.5695649203237778 1105 64-65 0.5153715309703308 1105 66-67 0.5412575967346918 1105 68-69 0.25796497535135643 1105 70-71 0.1840685583019166 1105 72-73 0.4849466052925422 1105 74-75 0.5341859426769453 1105 76-77 0.5210864140596314 1105 78-79 0.21964916979812088 1105 80-81 -0.2367345829633436 1105 82-83 -0.008223889629729086 1105 84-85 -0.04282430090625766 1105 86-87 -0.11872992915789737 1105 88-89 0.051495684918595686 1105 90-91 -0.04792266263709877 1105 92-93 -0.3887904434698477 1105 94-95 -0.030862938052038658 1105 96-97 1.1231331640318185 1105 98-99 1.3619145103101165 1105 100-101 0.949331522417161 1106 1 1.0257229242620838 1106 2 1.0942629947487923 1106 3 1.079704057060173 1106 4 1.1344024219881064 1106 5 1.0980030194605916 1106 6 1.2959170144547727 1106 7 1.1907532177445361 1106 8 1.1567577603205947 1106 9 1.4776570429458715 1106 10-11 1.4468420832913722 1106 12-13 1.3143627996428933 1106 14-15 1.4446406673568788 1106 16-17 1.6092216337438074 1106 18-19 1.473820498751806 1106 20-21 1.4995454532943668 1106 22-23 1.1958274401044164 1106 24-25 1.3053329463752306 1106 26-27 1.7460042278237893 1106 28-29 1.5092456805523042 1106 30-31 1.469654995612892 1106 32-33 1.6215947797741208 1106 34-35 1.66057846665273 1106 36-37 1.584574224409657 1106 38-39 1.494335263353591 1106 40-41 1.4401456113302586 1106 42-43 1.349202533884828 1106 44-45 1.5948709767992888 1106 46-47 1.442229090071251 1106 48-49 1.3199033676610874 1106 50-51 1.5278020569970039 1106 52-53 1.6266842930678749 1106 54-55 1.2884178278787424 1106 56-57 1.1827788078324488 1106 58-59 1.2065257702073744 1106 60-61 1.6305797508664241 1106 62-63 1.6060630311043873 1106 64-65 1.463927986473184 1106 66-67 1.412603145364951 1106 68-69 1.521663293733294 1106 70-71 1.7375188072816456 1106 72-73 1.7551233028708424 1106 74-75 1.79892197214782 1106 76-77 1.5635134580342225 1106 78-79 1.1828625924772673 1106 80-81 1.3819989543457858 1106 82-83 1.4509705084446374 1106 84-85 1.565020067848561 1106 86-87 1.6148372315274244 1106 88-89 1.5562615895922285 1106 90-91 1.5657899017358652 1106 92-93 1.6331081865505368 1106 94-95 1.9335420346855088 1106 96-97 3.407149309882394 1106 98-99 3.66215305676978 1106 100-101 3.089088093306632 1107 1 0.6319411946753029 1107 2 0.6236893932241827 1107 3 0.5992146451314575 1107 4 0.34536765040207484 1107 5 0.671497583236139 1107 6 0.7830112580688962 1107 7 0.5688417494696907 1107 8 0.4861974387650747 1107 9 0.6866687338254422 1107 10-11 0.7456299706375802 1107 12-13 0.7068725665794346 1107 14-15 0.7777422522100252 1107 16-17 0.8666014057069091 1107 18-19 0.6341214432820976 1107 20-21 0.657721752996224 1107 22-23 0.5275690299330407 1107 24-25 0.7320043822133044 1107 26-27 0.8073650460862183 1107 28-29 0.6600526489907494 1107 30-31 0.8223271086692918 1107 32-33 0.9812092821012683 1107 34-35 0.765289220212857 1107 36-37 0.6652847388101648 1107 38-39 0.6312951599500991 1107 40-41 0.6690113066763459 1107 42-43 0.6224260300560189 1107 44-45 0.6518495554880595 1107 46-47 0.5840148433083954 1107 48-49 0.5169462610598892 1107 50-51 0.7398700937038001 1107 52-53 0.7439602409106492 1107 54-55 0.7106816359669672 1107 56-57 0.6769788864907262 1107 58-59 0.4957486246135474 1107 60-61 0.5329219528355225 1107 62-63 0.3520376531435474 1107 64-65 0.2585332844597623 1107 66-67 0.3248580029943682 1107 68-69 0.2060370365303399 1107 70-71 0.2858880901723282 1107 72-73 0.11674377156962379 1107 74-75 0.27108120799075763 1107 76-77 0.20751384141070872 1107 78-79 0.06031506776435691 1107 80-81 0.09034259067172812 1107 82-83 0.23156634039714774 1107 84-85 0.09857512458737716 1107 86-87 0.0866692753717544 1107 88-89 -0.13932606556483051 1107 90-91 -0.10998325836498068 1107 92-93 0.036435119259248694 1107 94-95 0.15314720498007972 1107 96-97 1.556908602931351 1107 98-99 1.8633091351648634 1107 100-101 1.3938340898616524 1108 1 0.6507759354539253 1108 2 0.6948537221154361 1108 3 0.6604250342524018 1108 4 0.7890826778408879 1108 5 0.8761192499729233 1108 6 0.8899918153544206 1108 7 0.690244497676737 1108 8 0.7579012406154249 1108 9 0.9310477917980506 1108 10-11 0.7682947679530159 1108 12-13 0.6968589256880477 1108 14-15 0.7343678322590108 1108 16-17 0.8105564227639448 1108 18-19 0.6351984337293288 1108 20-21 0.6380322343798497 1108 22-23 0.6699956094054542 1108 24-25 0.8890095902843029 1108 26-27 0.9161896717468494 1108 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1.6768289417131967 2311 78-79 1.740057662684567 2311 80-81 1.6135796778315843 2311 82-83 1.7540575468328292 2311 84-85 1.8391673961020345 2311 86-87 2.210106671380178 2311 88-89 2.177756362171376 2311 90-91 2.414569088593872 2311 92-93 2.404050086481835 2311 94-95 2.7806733115384077 2311 96-97 4.09698297990545 2311 98-99 4.389235673536527 2311 100-101 4.160779054477761 2312 1 0.24970790539624588 2312 2 -0.1891464477019511 2312 3 -0.797884191077074 2312 4 -0.1528567580014979 2312 5 -2.025652309253921 2312 6 -0.3779254350595096 2312 7 0.10129779001130146 2312 8 0.423573262594644 2312 9 0.43500211092130314 2312 10-11 0.24742481774589464 2312 12-13 0.35085842836571146 2312 14-15 0.5854971178736577 2312 16-17 -0.1944137682199667 2312 18-19 0.1541934180515412 2312 20-21 0.4280660765588493 2312 22-23 0.32212044944353835 2312 24-25 0.5043410677562079 2312 26-27 0.9419327744317911 2312 28-29 0.6691071158318849 2312 30-31 0.5737272177838619 2312 32-33 -0.13541756066580746 2312 34-35 0.23826250464885135 2312 36-37 0.583091711055971 2312 38-39 0.761339885252724 2312 40-41 0.788385415014865 2312 42-43 0.797683426652469 2312 44-45 0.7403241104960934 2312 46-47 0.5884291694092383 2312 48-49 0.8922294775472785 2312 50-51 0.9708535252110693 2312 52-53 0.6086393594750028 2312 54-55 0.7934394920699432 2312 56-57 0.9649751170783993 2312 58-59 0.9176047976097337 2312 60-61 1.2398699833759323 2312 62-63 1.3148810795743628 2312 64-65 1.3228315231639272 2312 66-67 1.4997025730858908 2312 68-69 1.3057912370035574 2312 70-71 1.4720535883795236 2312 72-73 1.3409196502482317 2312 74-75 1.5327520904475271 2312 76-77 1.564841293060411 2312 78-79 1.4849933402531867 2312 80-81 1.2547082236675031 2312 82-83 1.3476090993035506 2312 84-85 1.4089691623806964 2312 86-87 1.3576183970578377 2312 88-89 1.299093494534251 2312 90-91 1.460887090772463 2312 92-93 1.0510405709031119 2312 94-95 1.4504903819644923 2312 96-97 2.9760442054975886 2312 98-99 3.199633491345155 2312 100-101 2.8029617859980043 2313 1 -0.27262307191937296 2313 2 -0.6749542163928197 2313 3 -0.9764425796102998 2313 4 -0.22606414923612306 2313 5 -0.37131587979259706 2313 6 -0.03518036604422292 2313 7 -0.07573473783534723 2313 8 -0.08999850959941114 2313 9 0.3358951773215324 2313 10-11 -0.7849590157786253 2313 12-13 0.0065458674608009915 2313 14-15 0.07510473607543133 2313 16-17 -0.20232523869572816 2313 18-19 0.1637411489967846 2313 20-21 -2.1572325579376965 2313 22-23 -0.28650337717328966 2313 24-25 0.13413821586460983 2313 26-27 0.23170691744576288 2313 28-29 -0.2070724349211801 2313 30-31 0.21927422095399862 2313 32-33 0.28009453396962414 2313 34-35 0.12299372126680197 2313 36-37 -0.7731862743638587 2313 38-39 -0.02886197982518013 2313 40-41 0.36836450689955313 2313 42-43 0.06895635465207306 2313 44-45 0.14886714174108917 2313 46-47 0.13860957128911977 2313 48-49 -0.04314748528685186 2313 50-51 0.3032352000806249 2313 52-53 0.27925266419073935 2313 54-55 0.6264598204321992 2313 56-57 0.5443189476424841 2313 58-59 0.5955396432350675 2313 60-61 0.3911849126337259 2313 62-63 0.5211128081019147 2313 64-65 0.4312555200061219 2313 66-67 0.5051170965165497 2313 68-69 0.4391079178556687 2313 70-71 0.7885059314453073 2313 72-73 0.508029766322192 2313 74-75 0.6589772813343053 2313 76-77 0.5111299484033864 2313 78-79 0.44730106577095796 2313 80-81 0.772408577686921 2313 82-83 0.7144746706070073 2313 84-85 0.6730886161793102 2313 86-87 0.715475592779999 2313 88-89 1.337977038182249 2313 90-91 1.4010667525420644 2313 92-93 1.7548064986930214 2313 94-95 2.084724182800816 2313 96-97 3.2314562618939906 2313 98-99 3.352217092968461 2313 100-101 2.9989389782663167 2314 1 -0.3713187968354248 2314 2 -0.3970682046891767 2314 3 -1.3012562219211752 2314 4 -0.42985214784015824 2314 5 -0.6387259294487677 2314 6 -0.5442946719623194 2314 7 -0.5186657590218928 2314 8 -1.9495080781174785 2314 9 -1.624265515747389 2314 10-11 -0.9078281399399728 2314 12-13 -0.768159515508664 2314 14-15 -0.6677951764805599 2314 16-17 -0.8705760383051668 2314 18-19 -1.7830446301763772 2314 20-21 -1.5309156694804358 2314 22-23 -2.239263516560875 2314 24-25 -1.8591549659862494 2314 26-27 -1.920960647624213 2314 28-29 -2.8087375309069884 2314 30-31 -1.656718673730218 2314 32-33 -1.3389636195632235 2314 34-35 -1.7461972648114354 2314 36-37 -3.3835879286110355 2314 38-39 -3.9055229584922415 2314 40-41 -4.491505327009115 2314 42-43 -3.11545426750747 2314 44-45 -3.461115101504877 2314 46-47 -3.526872168075453 2314 48-49 -1.9732414581857434 2314 50-51 -1.3962423579180196 2314 52-53 -2.53804556431653 2314 54-55 -1.8455693707041938 2314 56-57 -1.7682974646435916 2314 58-59 -3.769544970932955 2314 60-61 -2.3022960647001085 2314 62-63 -2.050172030623365 2314 64-65 -1.6058207719496806 2314 66-67 -2.5295814438612787 2314 68-69 -2.557306747118755 2314 70-71 -1.919115911780267 2314 72-73 -1.3334681665223798 2314 74-75 -1.8578932688571221 2314 76-77 -1.0365072661455201 2314 78-79 -0.7448445605255074 2314 80-81 -0.6217309092298073 2314 82-83 -0.40728441134854876 2314 84-85 -0.2583877628283737 2314 86-87 0.16047229892499715 2314 88-89 0.4404525270648385 2314 90-91 1.0047625689540887 2314 92-93 1.1774012329691104 2314 94-95 1.7731179840794056 2314 96-97 3.378034770012947 2314 98-99 3.5329896065327393 2314 100-101 3.1012813078321955 2315 1 -2.390396291873472 2315 2 -1.8644524782168652 2315 3 -1.5044846572227542 2315 4 -1.6761013669679485 2315 5 -1.5764543540274119 2315 6 -2.9131115995423436 2315 7 -2.3393920272517583 2315 8 -1.3891164763246024 2315 9 -1.3054742194545454 2315 10-11 -3.304998943932226 2315 12-13 -2.0078015007331373 2315 14-15 -1.0123151311813245 2315 16-17 -2.0654843364084527 2315 18-19 -1.294810636240726 2315 20-21 -0.799319002862191 2315 22-23 -1.0532874280267421 2315 24-25 -2.930482756596966 2315 26-27 -4.31785334696195 2315 28-29 -5.884245020016287 2315 30-31 -8.361408023221294 2315 32-33 -6.637098035429304 2315 34-35 -6.040236036197648 2315 36-37 -3.6016880449030566 2315 38-39 -6.325063546206238 2315 40-41 -4.067166223350103 2315 42-43 -3.900010327387136 2315 44-45 -2.874285051812695 2315 46-47 -1.9715606458952237 2315 48-49 -2.43763420422016 2315 50-51 -2.4173048076326147 2315 52-53 -1.8679191927313532 2315 54-55 -3.3296141538552 2315 56-57 -2.981455342995634 2315 58-59 -1.8071831844495314 2315 60-61 -2.044197823193194 2315 62-63 -2.4635038627450037 2315 64-65 -1.7841303038137646 2315 66-67 -2.3087497449116405 2315 68-69 -1.5690388548545613 2315 70-71 -2.1144565865230405 2315 72-73 -1.3102917409420094 2315 74-75 -1.8374155653097652 2315 76-77 -1.0085688172085057 2315 78-79 -0.836581566223888 2315 80-81 -0.6971524418631745 2315 82-83 -0.5887874770718753 2315 84-85 -0.3295152765796807 2315 86-87 -0.9281602425018711 2315 88-89 -1.426644406243316 2315 90-91 -0.7776521888561874 2315 92-93 0.08572337639501626 2315 94-95 0.6392960305533215 2315 96-97 2.032681884953883 2315 98-99 1.7660034308245578 2315 100-101 1.55245059262589 2316 1 -3.5427816104253296 2316 2 -2.3655533540364218 2316 3 -2.482770144510493 2316 4 -3.019324560153631 2316 5 -2.5352785195282443 2316 6 -6.264114139967237 2316 7 -3.6351799381934597 2316 8 -4.721669960040874 2316 9 -6.258430551630685 2316 10-11 -5.380409688510262 2316 12-13 -3.711595504789713 2316 14-15 -4.8568158584652465 2316 16-17 -4.562161996814549 2316 18-19 -2.739903328332659 2316 20-21 -3.4824505590245067 2316 22-23 -3.795860646406407 2316 24-25 -2.611123825272543 2316 26-27 -3.67508679946922 2316 28-29 -3.4889592616397564 2316 30-31 -3.356994945477382 2316 32-33 -3.222873983920934 2316 34-35 -5.528878654810256 2316 36-37 -5.7377385740518605 2316 38-39 -5.49807526760506 2316 40-41 -5.150172147503817 2316 42-43 -4.347202080280319 2316 44-45 -3.7658827635529413 2316 46-47 -3.557024767180799 2316 48-49 -4.13888440415753 2316 50-51 -5.5432148512095765 2316 52-53 -3.212308217915581 2316 54-55 -3.402162133192377 2316 56-57 -2.720082567369296 2316 58-59 -3.220078825777282 2316 60-61 -3.165978831508337 2316 62-63 -2.5138507212313996 2316 64-65 -2.766692198041074 2316 66-67 -3.397156981856014 2316 68-69 -2.8136718974996064 2316 70-71 -2.27618977507543 2316 72-73 -2.091897803118215 2316 74-75 -3.039877592227903 2316 76-77 -3.169382293850937 2316 78-79 -3.138594944943943 2316 80-81 -3.141396573466647 2316 82-83 -2.0599202752864407 2316 84-85 -2.0002811287033744 2316 86-87 -1.6776079272458801 2316 88-89 -1.7941899337663365 2316 90-91 -1.055068952957125 2316 92-93 -0.878671813994174 2316 94-95 -0.6187613516600976 2316 96-97 0.8471841216241582 2316 98-99 1.1681349321683623 2316 100-101 0.8582990141907416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14044.0 3 4494.0 4 4537.0 5 4957.0 6 5898.0 7 6984.0 8 8925.0 9 10703.0 10 13103.0 11 15538.0 12 18602.0 13 21197.0 14 22871.0 15 26108.0 16 29556.0 17 31550.0 18 28469.0 19 26662.0 20 28675.0 21 30854.0 22 34663.0 23 38688.0 24 42733.0 25 49091.0 26 54410.0 27 61186.0 28 68764.0 29 78089.0 30 89190.0 31 101008.0 32 114789.0 33 130541.0 34 146435.0 35 155169.0 36 141781.0 37 91430.0 38 19088.0 39 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.69670416643112 17.97501271976935 13.511447792413364 22.816835321386172 2 16.69166174396486 20.179874110733937 38.371975364821665 24.756488780479533 3 19.799848594581178 24.63266659543036 29.265118351207427 26.302366458781034 4 13.284962831574193 15.966449749543557 35.411300988198946 35.3372864306833 5 13.982450694525722 37.563361654897584 32.76955949196462 15.684628158612067 6 32.810967200963006 34.98520597143873 16.82730989546081 15.376516932137447 7 29.150407218940334 33.34626361142667 19.611707141641396 17.891622027991602 8 28.257571516700846 32.4333631325477 19.269008873606662 20.040056477144795 9 28.257610586990538 15.545938353015423 19.21470696843797 36.98174409155607 10-11 26.10983316595645 25.795351220041802 25.932645504799645 22.16217010920211 12-13 27.396784279731307 23.44316565435008 26.682296009230587 22.47775405668803 14-15 23.939415955426735 24.579213861609496 25.283890532138738 26.197479650825034 16-17 22.87187260849026 27.800782132349593 26.164116618178003 23.16322864098214 18-19 23.009827440149206 25.368684520961775 29.264005148406902 22.35748289048212 20-21 23.584857363555702 24.52369901290529 29.425995049677176 22.46544857386183 22-23 23.278691214365388 24.618741868676167 29.646124221026604 22.45644269593184 24-25 22.951837347241412 25.10207956249878 29.57931987325186 22.36676321700795 26-27 22.396458778749505 25.266712090155817 29.56639054165247 22.770438589442207 28-29 22.498793191164744 25.880970449736484 29.311929383149497 22.30830697594928 30-31 22.342132903541255 25.440336921976442 29.14024796044947 23.07728221403283 32-33 21.982792161030556 25.616658559905286 29.025035320296304 23.37551395876785 34-35 23.080641744024845 25.552341196867257 28.99711726054041 22.369899798567484 36-37 23.23885998418923 24.985709497633334 28.91642495612441 22.859005562053024 38-39 22.656320498498907 25.300098186950105 28.820684491185943 23.222896823365044 40-41 22.818431081717865 25.355408815598107 29.183234309318102 22.64292579336593 42-43 22.751014307792914 25.551137405046536 28.608938806635486 23.088909480525064 44-45 22.545259728981897 25.600563692309088 29.00342322577506 22.850753352933957 46-47 23.201380228197124 26.050420881327085 28.00798871010455 22.740210180371246 48-49 22.737314526729232 26.26928306542553 27.610930242100363 23.382472165744872 50-51 22.7890446984026 26.532708912878405 27.75581375274071 22.92243263597829 52-53 23.267683668885184 26.062076030866184 27.712815060215895 22.957425240032737 54-55 22.428011612345138 26.33639342480395 27.90359579618613 23.33199916666478 56-57 22.5665831609312 26.600778915240404 27.48029154283001 23.352346380998384 58-59 22.621268128818457 25.90417729627928 27.580986895866396 23.893567679035876 60-61 22.078561335466322 26.231497236873558 28.1597874931576 23.53015393450252 62-63 22.358753841984882 26.20288848244638 28.177921874407758 23.260435801160984 64-65 21.931392575052453 26.502477377210166 27.829467976243876 23.7366620714935 66-67 21.972072701931584 26.49806886908358 27.73673040416862 23.79312802481622 68-69 22.800564778715806 25.911146912610906 27.849971602672742 23.43831670600055 70-71 22.38608396764979 26.1338851563585 27.454600327597777 24.02543054839393 72-73 22.39512225350168 26.3387388723154 27.30696521396874 23.959173660214184 74-75 22.3710629227709 26.85562165911411 27.06200701195156 23.711308406163432 76-77 22.50671085091665 26.267476233228532 27.292303268192885 23.93350964766193 78-79 22.58890984704945 26.57886879704089 27.123740093024047 23.708481262885613 80-81 22.879191783570707 26.600262473357144 26.82568842652119 23.694857316550962 82-83 22.667653439104775 26.567376227746326 26.86105895021941 23.903911382929497 84-85 22.404792323679924 26.77074181129801 26.786135198649685 24.03833066637238 86-87 22.8349613563803 26.59257096358367 26.473114701778094 24.099352978257944 88-89 22.603077126796446 26.61100407976638 26.136394181430912 24.649524612006264 90-91 22.441125123437054 26.668854815389096 26.38757395612573 24.502446105048115 92-93 22.94072921020291 26.219710447255224 26.465753595215304 24.37380674732656 94-95 22.574685099655124 25.91248301727039 26.81840782152152 24.69442406155297 96-97 22.9449558675324 25.41970142127481 26.68394671634217 24.95139599485062 98-99 22.69208748879434 25.821092290450654 26.221098179968955 25.26572204078605 100-101 22.930448924619277 25.590858336441286 26.463266238321097 25.015426500618332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3516.0 1 2247.5 2 1136.5 3 1339.0 4 1186.5 5 1097.0 6 1698.5 7 3190.0 8 4800.5 9 5171.0 10 4068.0 11 2975.0 12 2721.0 13 2710.5 14 2822.0 15 2903.0 16 2898.5 17 3025.5 18 3310.0 19 3587.5 20 3884.5 21 4420.0 22 5244.0 23 6121.0 24 7118.0 25 8464.0 26 10128.5 27 12163.0 28 14003.0 29 15571.5 30 17649.0 31 21307.5 32 26549.0 33 27540.5 34 25680.5 35 26519.5 36 28830.0 37 31337.0 38 33794.0 39 35818.0 40 37729.5 41 40672.0 42 42788.0 43 45170.5 44 48806.5 45 53062.0 46 56449.0 47 59223.5 48 62564.5 49 63591.0 50 63259.0 51 64071.5 52 66112.0 53 68997.0 54 70546.5 55 68764.0 56 65556.5 57 61620.5 58 57673.0 59 52025.5 60 43761.0 61 36388.5 62 30151.5 63 23167.0 64 16848.5 65 12976.5 66 10185.0 67 7896.0 68 5943.0 69 4345.5 70 3214.5 71 2407.5 72 1813.5 73 1462.0 74 1237.5 75 1015.5 76 778.5 77 585.0 78 424.0 79 271.0 80 172.5 81 136.0 82 96.5 83 56.0 84 37.0 85 27.5 86 21.0 87 11.5 88 9.5 89 9.0 90 4.5 91 4.0 92 4.0 93 3.0 94 2.5 95 3.5 96 2.5 97 1.5 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11558787473594635 2 0.10909417390808421 3 0.12343678269310149 4 0.1341655058000042 5 0.13156802546885932 6 0.13145509154141824 7 0.14048980573670472 8 0.13800525933300092 9 0.14613650210875878 10-11 0.140207470918102 12-13 0.1399533695813596 14-15 0.12781297238144337 16-17 0.1310315893135142 18-19 0.14867751547618308 20-21 0.17976257900434062 22-23 0.19630739937445899 24-25 0.2157320348943249 26-27 0.21869655048965328 28-29 0.21965648887290246 30-31 0.21971295583662298 32-33 0.22041879288312977 34-35 0.18958783069171467 36-37 0.17868970669365034 38-39 0.1920159101316979 40-41 0.1803554821234063 42-43 0.18289649549083062 44-45 0.18724445169731224 46-47 0.20909716665716138 48-49 0.22668662585610977 50-51 0.24255384266158161 52-53 0.2641242228028281 54-55 0.2569811518921797 56-57 0.22600902229146327 58-59 0.2185836165622122 60-61 0.19619446544701788 62-63 0.18667978206010682 64-65 0.1778709357197025 66-67 0.1734100455857798 68-69 0.17928260981271602 70-71 0.18241652629920602 72-73 0.22030585895568866 74-75 0.2318815865183995 76-77 0.2487369751889808 78-79 0.23713301414440974 80-81 0.24232797480669946 82-83 0.23967402751183406 84-85 0.22329860803287732 86-87 0.21304985411759922 88-89 0.21522383222084004 90-91 0.21751074425152192 92-93 0.22476674908961136 94-95 0.23030051153422434 96-97 8.698933395522284 98-99 0.2878685810473154 100-101 0.30020661262025344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1770947.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.90764382584113 #Duplication Level Percentage of deduplicated Percentage of total 1 75.04396497324512 36.702235101903696 2 12.500899684320046 12.227790985265893 3 4.8101008576263995 7.057520985334946 4 2.410674414706949 4.716016226182219 5 1.347806537059105 3.2959021030313522 6 0.8897258090234829 2.61086357822273 7 0.6150995769194963 2.1058149717783006 8 0.41081358600355383 1.6073539634462688 9 0.3076935076474012 1.3543708031588542 >10 1.4921663822227078 12.886595478797128 >50 0.09759879852419102 3.2874162607213044 >100 0.06360679054272914 5.7724456267282145 >500 0.005458527461445381 1.825219325313113 >1k 0.0040345637758509335 3.1433816952920237 >5k 2.3732728093240788E-4 0.782261726101604 >10k+ 1.1866364046620394E-4 0.6248111687224475 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10766 0.6079233314153388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7740 0.43705429919698335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5739 0.3240639047921818 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2679 0.15127499580732795 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2642 0.14918571814966794 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2518 0.14218381464832092 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2472 0.13958633431717604 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2270 0.1281800076456269 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2232 0.12603426302424636 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1954 0.1103364471099361 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1931 0.10903770694436365 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 1910 0.10785190070623232 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1890 0.1067225614318215 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.646696372054048E-5 7 0.0 0.0 0.0 0.0 5.646696372054048E-5 8 0.0 0.0 0.0 0.0 5.646696372054048E-5 9 0.0 0.0 0.0 5.646696372054048E-5 5.646696372054048E-5 10-11 0.0 0.0 0.0 5.646696372054048E-5 5.646696372054048E-5 12-13 0.0 0.0 0.0 5.646696372054048E-5 5.646696372054048E-5 14-15 0.0 0.0 0.0 1.1293392744108096E-4 5.646696372054048E-5 16-17 0.0 0.0 0.0 1.6940089116162144E-4 5.646696372054048E-5 18-19 0.0 0.0 0.0 2.2586785488216192E-4 5.646696372054048E-5 20-21 0.0 0.0 0.0 2.823348186027024E-4 5.646696372054048E-5 22-23 0.0 0.0 0.0 0.0010164053469697287 5.646696372054048E-5 24-25 0.0 0.0 0.0 0.0018916432846381062 5.646696372054048E-5 26-27 0.0 0.0 0.0 0.004150321833459725 5.646696372054048E-5 28-29 0.0 0.0 0.0 0.010954590961784853 1.1293392744108096E-4 30-31 0.0 0.0 0.0 0.03421898001464753 1.1293392744108096E-4 32-33 0.0 0.0 0.0 0.07911021617247721 1.1293392744108096E-4 34-35 0.0 0.0 0.0 0.14384959007807685 1.1293392744108096E-4 36-37 0.0 0.0 0.0 0.25091095329222163 1.1293392744108096E-4 38-39 0.0 0.0 0.0 0.41398754451714254 1.1293392744108096E-4 40-41 0.0 0.0 0.0 0.5958394011791431 1.1293392744108096E-4 42-43 0.0 0.0 0.0 0.7783123944420697 1.1293392744108096E-4 44-45 0.0 0.0 0.0 0.9962466409214957 1.1293392744108096E-4 46-47 0.0 0.0 0.0 1.2581404186573626 1.1293392744108096E-4 48-49 0.0 0.0 0.0 1.5282219061327076 1.1293392744108096E-4 50-51 0.0 0.0 0.0 1.8381973034766146 1.6940089116162144E-4 52-53 0.0 0.0 0.0 2.1235531046383658 1.6940089116162144E-4 54-55 0.0 0.0 0.0 2.418734157487491 1.6940089116162144E-4 56-57 0.0 0.0 0.0 2.7622791647632594 1.6940089116162144E-4 58-59 0.0 0.0 0.0 3.1308390369672274 1.6940089116162144E-4 60-61 0.0 0.0 0.0 3.531359210637021 1.6940089116162144E-4 62-63 0.0 0.0 0.0 3.8973780694735645 2.2586785488216192E-4 64-65 0.0 0.0 0.0 4.266276743459855 2.2586785488216192E-4 66-67 0.0 0.0 0.0 4.694268094979692 2.2586785488216192E-4 68-69 0.0 0.0 0.0 5.165936642937366 2.2586785488216192E-4 70-71 0.0 0.0 0.0 5.6477974778465985 2.2586785488216192E-4 72-73 0.0 0.0 0.0 6.147586573737103 2.2586785488216192E-4 74-75 0.0 0.0 0.0 6.616290606099448 2.2586785488216192E-4 76-77 0.0 0.0 0.0 7.112635217203 2.2586785488216192E-4 78-79 0.0 0.0 0.0 7.672505162492158 2.2586785488216192E-4 80-81 0.0 0.0 0.0 8.252844382130014 2.2586785488216192E-4 82-83 0.0 0.0 0.0 8.841314844543625 2.2586785488216192E-4 84-85 0.0 0.0 0.0 9.418068411985226 2.2586785488216192E-4 86-87 0.0 0.0 0.0 9.929235601065418 2.2586785488216192E-4 88-89 5.646696372054048E-5 0.0 0.0 10.42140165685365 2.2586785488216192E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5725 0.0 42.887 1 GTATCAA 9950 0.0 39.803356 1 TAAGGTA 480 0.0 31.361784 4 TATCAAC 12715 0.0 31.038506 2 TCAACGC 12950 0.0 30.51417 4 ATCAACG 12985 0.0 30.50438 3 CAACGCA 13235 0.0 29.820696 5 AACGCAG 13675 0.0 28.930819 6 GTACATG 9735 0.0 27.975204 1 TACATGG 9835 0.0 27.40536 2 ACATGGG 9815 0.0 26.121508 3 ACGCAGA 15130 0.0 25.837717 7 AGGTAAG 880 0.0 25.657467 2 CGCAGAG 15230 0.0 25.45185 8 GCAGAGT 15515 0.0 23.589561 9 TAAGGTG 1135 0.0 23.209827 5 AAGGTAA 930 0.0 22.759369 1 GAGTACT 8810 0.0 22.641253 12-13 GTGTAAG 645 0.0 22.606464 1 GTAAGGT 1190 0.0 22.533049 4 >>END_MODULE