FastQCFastQC Report
Fri 27 May 2016
SRR939736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770947
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112530.635422742747242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91070.5142446386029622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71180.40193184776280716No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31650.17871794017551063No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28440.1605920448212171No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28160.159010969837042No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27520.1553970841589274No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27100.1530254716826647No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26970.15229140115429765No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24480.13823112718788308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.1261471969516874No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG21030.11875002470429663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.11276452654991934No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19300.10898123998064313No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA18680.1054802882299696No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT18390.10384274628207393No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA18140.10243107218906042No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT17860.1008499972048853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60750.042.9523351
GTATCAA96900.036.818361
TAAGGTG11600.034.752355
AGGTAAG10600.034.4553872
AAGGTAA10950.031.1952691
GGTAAGG13350.029.1310273
GTAAGGT13800.028.18114
TATCAAC127000.028.0857722
TCAACGC128800.027.6533184
ATCAACG129450.027.5144653
CAACGCA131500.027.0855335
GTACATG97650.026.7700821
TACATGG99300.026.3193152
AACGCAG136400.026.1125186
GGTGTGC17400.025.348778
ACATGGG102000.024.3178253
GTGTAAG6750.023.194261
GTGTGCA19750.022.8128599
ACGCAGA155700.022.7843277
CGCAGAG155500.022.7221348
Produced by FastQC (version 0.11.5)