##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939736_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1770947 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.280139383053246 31.0 31.0 34.0 30.0 34.0 2 31.50234987269523 31.0 31.0 34.0 30.0 34.0 3 31.527067156724623 31.0 31.0 34.0 30.0 34.0 4 35.3741896284869 37.0 35.0 37.0 33.0 37.0 5 35.098267198284304 37.0 35.0 37.0 32.0 37.0 6 35.137104611261655 37.0 35.0 37.0 32.0 37.0 7 35.03479211969641 37.0 35.0 37.0 32.0 37.0 8 34.985791217919 37.0 35.0 37.0 32.0 37.0 9 36.53800763094548 39.0 35.0 39.0 32.0 39.0 10-11 36.39626369394454 38.0 35.0 39.0 32.0 39.0 12-13 36.309290735408794 38.0 35.0 39.0 32.0 39.0 14-15 37.25577473521229 39.0 36.0 41.0 31.5 41.0 16-17 37.182994465672884 39.0 36.0 40.0 31.0 41.0 18-19 37.1948282472598 39.0 36.0 40.0 31.0 41.0 20-21 37.1171565269881 39.0 36.0 40.0 31.0 41.0 22-23 36.98459496529258 39.0 36.0 40.0 31.0 41.0 24-25 36.791926579395096 38.5 35.5 40.0 30.5 41.0 26-27 36.438935496093336 38.0 35.0 40.0 30.0 41.0 28-29 36.17348881700017 38.0 35.0 40.0 29.5 41.0 30-31 35.8246455145185 38.0 34.0 40.0 29.0 41.0 32-33 35.69250293769379 38.0 34.0 40.0 29.0 41.0 34-35 35.482192013651456 38.0 34.0 40.0 27.5 41.0 36-37 35.437901303652794 38.0 34.0 40.0 27.0 41.0 38-39 35.26594076502572 38.0 34.0 40.0 27.0 41.0 40-41 35.11765541261258 38.0 34.0 40.0 26.5 41.0 42-43 34.80555685743278 38.0 33.0 40.0 25.5 41.0 44-45 34.46170043485209 38.0 33.0 40.0 24.5 41.0 46-47 34.05033662780422 37.5 33.0 40.0 23.5 41.0 48-49 33.63124305809265 37.0 32.5 40.0 22.5 41.0 50-51 33.31893670448636 37.0 32.0 40.0 21.5 41.0 52-53 32.998219031964254 36.5 31.5 40.0 20.0 41.0 54-55 32.669398350148256 36.0 31.0 39.0 19.0 41.0 56-57 32.235967253678396 35.5 31.0 39.0 17.5 40.0 58-59 31.80629968034052 35.0 30.5 39.0 14.5 40.0 60-61 31.431452211726267 35.0 30.0 38.0 13.0 40.0 62-63 31.091858762571665 35.0 29.5 38.0 9.5 40.0 64-65 30.605198518081004 34.5 29.0 37.5 7.5 40.0 66-67 29.639608356433026 34.0 27.5 37.0 4.5 39.5 68-69 27.68103308568805 33.0 23.0 36.0 2.0 39.0 70-71 27.005883292950045 32.0 21.5 35.0 2.0 38.0 72-73 27.569953533335557 32.0 24.5 35.0 2.0 38.0 74-75 27.692679679290233 32.0 25.5 35.0 2.0 37.0 76-77 27.334037099924505 31.5 25.5 34.5 2.0 36.0 78-79 27.872554627552375 33.0 26.0 35.0 2.0 36.0 80-81 27.702439993969328 33.0 26.0 35.0 2.0 36.0 82-83 27.261081782797564 33.0 25.5 35.0 2.0 36.0 84-85 26.910872262128677 32.5 25.0 35.0 2.0 35.0 86-87 26.540198549137834 32.0 24.5 35.0 2.0 35.0 88-89 26.056432801207492 32.0 23.5 34.0 2.0 35.0 90-91 25.546498568280136 32.0 22.0 34.0 2.0 35.0 92-93 25.035177506723805 31.0 19.5 34.0 2.0 35.0 94-95 24.177897757527468 31.0 15.5 34.0 2.0 35.0 96-97 23.614509920398522 31.0 4.5 34.0 2.0 35.0 98-99 22.90336582630649 30.5 2.0 34.0 2.0 35.0 100-101 21.431019110114534 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2412828708053638 1101 2 0.3024734892927121 1101 3 0.37415933049852157 1101 4 0.3162158619758486 1101 5 0.2614353572082919 1101 6 0.28278048773647413 1101 7 0.2060132577191638 1101 8 0.1920786286431877 1101 9 0.35016399930977826 1101 10-11 0.5010741316341623 1101 12-13 0.3885614299319258 1101 14-15 0.48000874697073925 1101 16-17 0.5517310651420573 1101 18-19 0.3836843485898882 1101 20-21 0.4727724071193862 1101 22-23 0.3880174633363822 1101 24-25 0.280722857903136 1101 26-27 0.3488561776796715 1101 28-29 0.5503890090233554 1101 30-31 0.2362605232199897 1101 32-33 0.2487689635636201 1101 34-35 0.2605967397838711 1101 36-37 0.4593526353325288 1101 38-39 0.6163489418629169 1101 40-41 0.4238589021230226 1101 42-43 0.1087649092641314 1101 44-45 0.15080290777689243 1101 46-47 0.13806608296530953 1101 48-49 0.21429795397144602 1101 50-51 -0.007531724408536888 1101 52-53 -0.0037005567851977617 1101 54-55 -0.3538298902383019 1101 56-57 -0.2304957629534634 1101 58-59 -0.21731276382024234 1101 60-61 -0.29319811062435264 1101 62-63 -0.2977658423016649 1101 64-65 -0.459185089889246 1101 66-67 -0.44967286783042226 1101 68-69 -8.842650430416882 1101 70-71 -6.478107632645692 1101 72-73 -1.9587639631552705 1101 74-75 -0.9641706422475451 1101 76-77 -0.6452804256677389 1101 78-79 -0.9337256461727712 1101 80-81 -0.9144566317375116 1101 82-83 -0.6878555281425562 1101 84-85 -0.6851776571007129 1101 86-87 -0.7506630715940013 1101 88-89 -0.6116476224201151 1101 90-91 -0.8960450700476059 1101 92-93 -0.9990882613238732 1101 94-95 -0.5434598998859315 1101 96-97 -1.0609365087394558 1101 98-99 -0.7859138285927258 1101 100-101 -0.6156076387048053 1102 1 0.14980797720437167 1102 2 0.08893142442615698 1102 3 0.19250151148709094 1102 4 0.17597858747471662 1102 5 0.23915666216264242 1102 6 0.21409398503674737 1102 7 0.18556448159863237 1102 8 0.2117274038772834 1102 9 0.1522330320732479 1102 10-11 0.36227425484759834 1102 12-13 0.2522562927517171 1102 14-15 0.24005917361940732 1102 16-17 0.28996955626503507 1102 18-19 0.1806043451771373 1102 20-21 0.21077211681435415 1102 22-23 0.1822128031650152 1102 24-25 0.29533617049406047 1102 26-27 0.23680452997702162 1102 28-29 0.16272846867926916 1102 30-31 0.051641482090865054 1102 32-33 0.058068475252838425 1102 34-35 -0.17228047116040557 1102 36-37 -0.08507012857081264 1102 38-39 0.1303323368583662 1102 40-41 0.018212192265174565 1102 42-43 0.20262872517972141 1102 44-45 0.29292411788349426 1102 46-47 0.04498370835879939 1102 48-49 0.08191636684504289 1102 50-51 0.06232153858676526 1102 52-53 0.023998365872891725 1102 54-55 -0.1272861489155943 1102 56-57 -0.09926209953666643 1102 58-59 -0.4939540675779206 1102 60-61 -0.3328036946748618 1102 62-63 -0.24912783827063478 1102 64-65 -0.2803785205744873 1102 66-67 0.10066652312734803 1102 68-69 -4.617139100210423 1102 70-71 -0.05532793981500461 1102 72-73 0.36456021781335224 1102 74-75 0.21175725351744035 1102 76-77 0.004461291497598552 1102 78-79 -0.021324635202123687 1102 80-81 -0.08720723866339242 1102 82-83 0.05548546170895463 1102 84-85 -0.03890547286560775 1102 86-87 -0.09478118142016001 1102 88-89 -0.2936608434873982 1102 90-91 -0.31378126698522024 1102 92-93 -0.4182078392957074 1102 94-95 -0.31403914706515224 1102 96-97 -0.7397727179762548 1102 98-99 -0.5597703647218495 1102 100-101 -0.755555162192362 1103 1 0.19918148985034279 1103 2 0.21374796103582128 1103 3 0.24165586424595986 1103 4 0.1281286787913345 1103 5 0.21791586300386 1103 6 0.060243190858869866 1103 7 0.16683073872409437 1103 8 0.17717651561562064 1103 9 0.3560943414982276 1103 10-11 0.21120640383205114 1103 12-13 0.33099501653278196 1103 14-15 0.2781971447301501 1103 16-17 0.3067651730672978 1103 18-19 0.2573006205659354 1103 20-21 0.308476472340395 1103 22-23 0.19790155185060598 1103 24-25 0.2822477317315375 1103 26-27 0.24724506348744768 1103 28-29 0.13780207540902722 1103 30-31 0.22622225579167576 1103 32-33 0.2962130875722764 1103 34-35 0.05564083051650215 1103 36-37 0.27132839929459607 1103 38-39 0.3804532622001062 1103 40-41 0.5354273120836481 1103 42-43 0.41943042975661626 1103 44-45 0.32628041873024927 1103 46-47 0.06002853651752105 1103 48-49 0.12316906483606971 1103 50-51 0.19690535617490212 1103 52-53 0.3450319039768246 1103 54-55 0.5035085823921719 1103 56-57 0.25216382331463194 1103 58-59 0.007905194141045513 1103 60-61 0.007241410200890641 1103 62-63 -0.08434229396824122 1103 64-65 -0.27824032809012067 1103 66-67 -0.028029309514192136 1103 68-69 -4.220960285666177 1103 70-71 0.131004729397457 1103 72-73 0.4855682979506071 1103 74-75 0.38138589926429844 1103 76-77 0.22559029697659483 1103 78-79 0.037691912476223166 1103 80-81 0.10578766974326825 1103 82-83 -0.05684643269582779 1103 84-85 -0.08933300067566918 1103 86-87 -0.092508504459623 1103 88-89 -0.10761845043852958 1103 90-91 0.028288022958882664 1103 92-93 -0.05755285008767075 1103 94-95 -0.12415509150037352 1103 96-97 -0.3691329482048218 1103 98-99 -0.15390728430788414 1103 100-101 -0.1411813728293687 1104 1 0.2415660394905501 1104 2 0.21568366724739363 1104 3 0.26695072791114427 1104 4 0.2122289241664319 1104 5 0.19973880695357593 1104 6 0.25527145034992316 1104 7 0.2654908252946342 1104 8 0.23517601935138543 1104 9 0.3373154272140937 1104 10-11 0.34124293439365516 1104 12-13 0.2960684912095175 1104 14-15 0.4205895518873817 1104 16-17 0.43130488799106104 1104 18-19 0.32726216008786224 1104 20-21 0.4688999511526717 1104 22-23 0.3120817509077014 1104 24-25 0.2086615333912647 1104 26-27 0.35300172859179924 1104 28-29 0.3166103519936243 1104 30-31 0.14307042650657564 1104 32-33 0.41288163820501467 1104 34-35 0.1635358430609628 1104 36-37 0.2906238419347247 1104 38-39 0.21961774622315744 1104 40-41 0.25957911379851595 1104 42-43 0.4390188061454694 1104 44-45 0.3516881825084752 1104 46-47 0.3688191681516102 1104 48-49 0.527788319888316 1104 50-51 0.4373962442121737 1104 52-53 0.4216552394238775 1104 54-55 0.4140784591532167 1104 56-57 0.411616691997601 1104 58-59 0.3618007305199704 1104 60-61 0.2702345727825701 1104 62-63 0.4572242467943539 1104 64-65 0.6038703112636306 1104 66-67 0.6948662544349702 1104 68-69 -4.405425698806152 1104 70-71 0.667242908659123 1104 72-73 1.2997599386552992 1104 74-75 0.8415212691190028 1104 76-77 0.7874587318143433 1104 78-79 0.4824940960388453 1104 80-81 0.3969906969550898 1104 82-83 0.16130374237516776 1104 84-85 0.19058277225587616 1104 86-87 0.22037685850252586 1104 88-89 0.4034675411249786 1104 90-91 0.29883749774590385 1104 92-93 0.05541412077058894 1104 94-95 0.15441084271704497 1104 96-97 -0.3021113607106791 1104 98-99 0.06844362964541517 1104 100-101 -0.18959691141391133 1105 1 0.1803701231984185 1105 2 0.14872584692701807 1105 3 0.09092941577183211 1105 4 0.10765654256842794 1105 5 0.18262174535680487 1105 6 0.17329383216802796 1105 7 0.16591853173603965 1105 8 0.14904184069302318 1105 9 0.35111092707983005 1105 10-11 0.23755204735261515 1105 12-13 0.10488046544919172 1105 14-15 0.17626603017907172 1105 16-17 0.23040460252328643 1105 18-19 0.27161121322404114 1105 20-21 0.23380873682513226 1105 22-23 0.1733847451279189 1105 24-25 0.2508866083348096 1105 26-27 0.3521737454865672 1105 28-29 0.37483989053507116 1105 30-31 0.40762705455328074 1105 32-33 0.022104839009692512 1105 34-35 0.1386173895611691 1105 36-37 0.1829670547567801 1105 38-39 0.2691297327883433 1105 40-41 0.13522441513539718 1105 42-43 0.19689173006621274 1105 44-45 0.086843775651964 1105 46-47 0.31384653740208535 1105 48-49 0.3508868888077643 1105 50-51 0.2300162584305454 1105 52-53 0.04712666293303158 1105 54-55 0.11798171374997679 1105 56-57 0.023527158122234937 1105 58-59 0.12183763553865745 1105 60-61 0.12828574898903966 1105 62-63 0.04126473831836108 1105 64-65 0.1255760142362803 1105 66-67 0.5791445073809882 1105 68-69 1.9045312004019337 1105 70-71 2.11981277893306 1105 72-73 1.1539954319621017 1105 74-75 0.8707412729927348 1105 76-77 0.2596371788872567 1105 78-79 0.16820717121129647 1105 80-81 0.28086506912407216 1105 82-83 0.3755645580162472 1105 84-85 0.15164720719695524 1105 86-87 -0.05424446200053268 1105 88-89 0.15067710462560058 1105 90-91 -0.06033150379918695 1105 92-93 -0.3009517886104476 1105 94-95 0.08155974706663116 1105 96-97 -0.2572178663826179 1105 98-99 -0.018877430260999972 1105 100-101 -0.002829426787794631 1106 1 0.4056350040522787 1106 2 0.43893914665321176 1106 3 0.3630582870164467 1106 4 0.34639127393987224 1106 5 0.5403131618220982 1106 6 0.45197704152379714 1106 7 0.5059741797690265 1106 8 0.4526289339433731 1106 9 0.664630609010004 1106 10-11 0.5665188760649684 1106 12-13 0.5597821503775222 1106 14-15 0.6427795278122233 1106 16-17 0.6582754061626943 1106 18-19 0.6807071602691579 1106 20-21 0.6797604222263658 1106 22-23 0.734429407787573 1106 24-25 0.6871470297797018 1106 26-27 0.6361086123427029 1106 28-29 0.8005961102992956 1106 30-31 1.0457140071170912 1106 32-33 0.9096006716139726 1106 34-35 1.065144725312578 1106 36-37 1.211539429120144 1106 38-39 1.2857121254527826 1106 40-41 1.061287869498571 1106 42-43 0.8580605870293709 1106 44-45 1.0715176606815007 1106 46-47 1.1926051736646954 1106 48-49 1.1962989703539435 1106 50-51 1.1981281444342429 1106 52-53 1.2087100427678124 1106 54-55 1.2613600766314192 1106 56-57 1.2358973481791296 1106 58-59 1.281390518312481 1106 60-61 1.1704918618787659 1106 62-63 1.2010879106936727 1106 64-65 1.3049677695315332 1106 66-67 1.7865809146957439 1106 68-69 3.159160408584924 1106 70-71 3.6351641642817967 1106 72-73 2.634514925916129 1106 74-75 2.285280991525056 1106 76-77 1.74831013822293 1106 78-79 1.6798374564148553 1106 80-81 1.6969207147925829 1106 82-83 1.664988860376468 1106 84-85 1.9667113378013603 1106 86-87 1.9016343442208878 1106 88-89 2.0490708248036 1106 90-91 1.740792835919816 1106 92-93 1.817740370301511 1106 94-95 2.1663950669571115 1106 96-97 2.1691876986571295 1106 98-99 2.3172644125200037 1106 100-101 2.246913879369785 1107 1 0.03381239439413619 1107 2 0.022564944765832706 1107 3 0.07087973813248993 1107 4 0.06371751741217935 1107 5 0.0935698939702263 1107 6 0.09285032267666793 1107 7 0.01358657653003803 1107 8 0.047351017540478324 1107 9 0.07448560797194403 1107 10-11 0.04451168385003257 1107 12-13 0.08594654377587574 1107 14-15 0.09080722706358557 1107 16-17 0.10615708584443695 1107 18-19 0.05325911664595395 1107 20-21 0.0755638919037196 1107 22-23 0.08289354330815968 1107 24-25 0.07905934833311079 1107 26-27 0.11074297002635802 1107 28-29 -0.021319654436140922 1107 30-31 0.08938858057319266 1107 32-33 0.11353247386502119 1107 34-35 0.34826510342514183 1107 36-37 0.12530330586159977 1107 38-39 0.06737531238697869 1107 40-41 -0.10003310885658578 1107 42-43 0.0026217636706888925 1107 44-45 0.0526497389876468 1107 46-47 0.060058463176133614 1107 48-49 0.20738984770621727 1107 50-51 0.11933391154624928 1107 52-53 0.14784992741234504 1107 54-55 0.04844521655633116 1107 56-57 0.07320676034219531 1107 58-59 -0.029339726757033446 1107 60-61 0.16465633700000382 1107 62-63 0.0982249356380791 1107 64-65 -0.07741151419740788 1107 66-67 0.4594117504234241 1107 68-69 1.8508140982380574 1107 70-71 1.967077312082143 1107 72-73 1.007791722234753 1107 74-75 0.6538390422659575 1107 76-77 0.29135616653570295 1107 78-79 0.34741849802712466 1107 80-81 0.22126031424828696 1107 82-83 0.3500697164597639 1107 84-85 0.20293735085719433 1107 86-87 0.22174965160860438 1107 88-89 0.14438930520257642 1107 90-91 0.08811886006231973 1107 92-93 0.10957910821170813 1107 94-95 0.6431449056896135 1107 96-97 0.5962527263392552 1107 98-99 0.993234368542879 1107 100-101 1.242842006494545 1108 1 -0.27001238829674534 1108 2 -0.19321564665154511 1108 3 -0.025776228750128638 1108 4 -0.04973801902979602 1108 5 -0.06044151768426076 1108 6 0.07101660052065029 1108 7 -0.08730209613775486 1108 8 0.031186679760722313 1108 9 -0.04708750323568722 1108 10-11 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0.12125240951753469 2316 78-79 -0.09725863170320892 2316 80-81 -0.23642427904762897 2316 82-83 0.034770061399246543 2316 84-85 -0.015689314149486933 2316 86-87 0.08542774880710624 2316 88-89 0.03143345336245673 2316 90-91 -0.1182302211113786 2316 92-93 -0.1837536837242446 2316 94-95 0.11288996457161105 2316 96-97 0.19942902870476686 2316 98-99 -0.16599468382490556 2316 100-101 -0.2758341896608876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 21.0 4 60.0 5 151.0 6 338.0 7 776.0 8 1828.0 9 3120.0 10 4924.0 11 7078.0 12 9544.0 13 11843.0 14 14557.0 15 17984.0 16 21660.0 17 24802.0 18 22292.0 19 18654.0 20 17946.0 21 18555.0 22 19367.0 23 21968.0 24 25823.0 25 30507.0 26 37170.0 27 45076.0 28 54398.0 29 64303.0 30 75810.0 31 89779.0 32 106204.0 33 127681.0 34 157632.0 35 194817.0 36 225057.0 37 206314.0 38 87796.0 39 5106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39598131178948 19.06278804966654 14.611178134432215 22.930052504111767 2 16.756770189703122 21.27693221790217 37.15188901113549 24.81440858125922 3 19.658081048208135 24.806289971574515 30.076975808409745 25.45865317180761 4 13.744962440999082 16.81541006026719 35.05056898935993 34.3890585093738 5 13.672515326545629 37.94212926756136 33.003585087526616 15.381770318366389 6 32.78511440489184 35.69423590881037 16.282983059346215 15.237666626951569 7 28.769635680796775 33.49129025318093 19.643106202500697 18.095967863521608 8 28.02043200615264 33.13876699867359 19.083857393812462 19.756943601361304 9 27.560056850939073 15.555519165734491 19.210286925582754 37.674137057743685 10-11 25.933243626150304 26.10445146015098 25.970342421314697 21.99196249238402 12-13 27.04996253416957 23.57272690825869 26.868336545362453 22.508974012209286 14-15 23.415325246887683 24.804412554412977 25.40719739212975 26.37306480656959 16-17 22.5062551490987 28.018861159123876 26.363695910818034 23.111187780959387 18-19 22.654941113426887 25.48088677978505 29.449526157473937 22.414645949314124 20-21 23.18626700855531 24.616264631296136 29.657126949592506 22.540341410556046 22-23 22.871124884030973 24.738233272932504 29.946040169468652 22.44460167356787 24-25 22.806721834952526 25.243387005242962 29.687539703378707 22.262351456425808 26-27 22.168180033050216 25.437175649223875 29.657896661182765 22.736747656543145 28-29 22.31344781643478 25.86748152328798 29.44986166993846 22.369208990338777 30-31 22.23552025225036 25.524340477001182 29.277575342798368 22.96256392795009 32-33 21.89834089917042 25.616113623645393 29.1788803314023 23.30666514578189 34-35 22.8945146035266 25.628558835794994 29.11853670808448 22.35838985259393 36-37 23.128689118591065 25.094428999906164 29.034500925353324 22.742380956149443 38-39 22.43750669437396 25.396624876816453 29.04681441061672 23.119054018192873 40-41 22.51884156690611 25.455398516981788 29.3419406990564 22.6838192170557 42-43 22.47006846540876 25.56846526743567 28.781051167173686 23.180415099981882 44-45 22.362951482037943 25.612339029957297 29.09944390368534 22.92526558431942 46-47 23.07616221562809 25.99946304931468 28.153228769252507 22.77114596580472 48-49 22.598187003808444 26.25009467416825 27.743459569325797 23.40825875269751 50-51 22.701270426039525 26.397641033003268 27.849358682695314 23.051729858261897 52-53 23.22610832205026 25.996094059036672 27.788746911752405 22.989050707160665 54-55 22.41757589797775 26.240161373254757 27.999305010142333 23.34295771862516 56-57 22.557645146922717 26.478342664427167 27.55003218316528 23.413980005484834 58-59 22.486840689680292 25.91414885995941 27.61084641734406 23.988164033016236 60-61 21.974592973678117 26.281448183558563 28.201940398809555 23.54201844395376 62-63 22.2909314625798 26.23085834191885 28.136744501480454 23.341465694020894 64-65 21.822249189727792 26.431716195929027 27.894596707636506 23.851437906706675 66-67 21.64702029476204 26.43494496649686 28.01857046661182 23.899464272129283 68-69 22.30407671086185 25.78127126949988 28.43681086820397 23.4778411514343 70-71 21.978201758489774 26.006623399085317 27.93568838268276 24.079486459742153 72-73 21.970388555091958 26.319486426413974 27.596065197570407 24.114059820923657 74-75 22.245631733431313 26.794379043730252 26.999585689307647 23.960403533530783 76-77 22.224249457659603 26.328977570083428 27.26572038705636 24.18105258520061 78-79 22.335635800579833 26.534005626663742 27.14921727031139 23.981141302445035 80-81 22.63240426873926 26.53108184950091 26.856759844171478 23.979754037588354 82-83 22.484621213746777 26.6226638192243 26.759031613612162 24.133683353416764 84-85 22.221469326609224 26.687401411848676 26.724472109593744 24.366657151948356 86-87 22.583931206747643 26.574632135271337 26.44424862762747 24.39718803035355 88-89 22.22811048002916 26.67551128790315 26.13054785172545 24.96583038034224 90-91 22.065355889785874 26.623966047304403 26.391856954619158 24.91882110829057 92-93 22.345803242752428 26.29345029061445 26.517714408976662 24.843032057656462 94-95 21.88649985602883 25.82707127039085 27.082347269761282 25.204081603819034 96-97 22.27196598723787 25.329748544232434 26.987548778555876 25.410736689973817 98-99 22.02778008393665 25.871516264632575 26.51052935664945 25.59017429478132 100-101 21.92602971111418 25.498298321362427 26.9263643148121 25.649307652711293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2018.0 1 1368.5 2 916.0 3 1228.5 4 1149.0 5 1125.5 6 1854.0 7 3549.5 8 5182.5 9 5445.5 10 4369.0 11 3288.0 12 3012.0 13 2921.0 14 2886.5 15 2965.5 16 3044.5 17 3113.5 18 3277.5 19 3616.5 20 3938.0 21 4319.5 22 5115.0 23 6021.5 24 7051.5 25 8435.5 26 10045.5 27 12022.0 28 13869.5 29 15530.5 30 17616.0 31 21229.5 32 27103.0 33 28070.0 34 25602.0 35 26321.0 36 28604.0 37 31165.5 38 33669.0 39 35613.0 40 37522.0 41 40646.0 42 42711.0 43 44912.0 44 48689.5 45 53305.5 46 56649.5 47 59043.0 48 62297.5 49 62950.5 50 62871.0 51 63781.0 52 65741.0 53 69554.0 54 72492.0 55 70854.0 56 66714.5 57 62662.0 58 58924.5 59 53139.0 60 44249.0 61 36663.0 62 30278.5 63 22419.5 64 15816.5 65 12261.0 66 9483.5 67 7411.5 68 5421.0 69 3725.5 70 2651.5 71 1978.0 72 1550.0 73 1313.5 74 1046.5 75 822.5 76 609.0 77 404.5 78 276.5 79 175.0 80 107.0 81 68.5 82 43.0 83 23.0 84 10.0 85 6.0 86 2.5 87 2.0 88 2.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023659657798906462 2 0.022078582814731327 3 5.646696372054048E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.388017823232429E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1293392744108096E-4 26-27 3.1056830046297264E-4 28-29 8.470044558081072E-5 30-31 4.5173570976432385E-4 32-33 0.002710414258585943 34-35 0.005110260216708913 36-37 0.10689196232298312 38-39 0.09811134946443908 40-41 0.10838833686157745 42-43 0.11019527970063474 44-45 0.10327807664486854 46-47 0.0958244374337572 48-49 0.09717964456305016 50-51 0.09588090439747773 52-53 0.07538339656692153 54-55 0.06420293775025453 56-57 0.0345295483151105 58-59 0.013693238702231067 60-61 0.009994652578535665 62-63 0.0021739781032408083 64-65 0.005166727180429454 66-67 0.16818685144162981 68-69 0.4437738678797276 70-71 0.42361516183149467 72-73 0.19698500293910548 74-75 0.03029452603606997 76-77 0.03447308135138997 78-79 0.005251427626010264 80-81 0.0014681410567340524 82-83 5.646696372054048E-5 84-85 3.670352641835131E-4 86-87 9.881718651094584E-4 88-89 1.411674093013512E-4 90-91 7.05837046506756E-4 92-93 0.002484546403703781 94-95 0.08444634424406829 96-97 0.0179282609812716 98-99 0.018408230172896195 100-101 0.011039291407365663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1770947.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.82359665928668 #Duplication Level Percentage of deduplicated Percentage of total 1 70.37780487625346 30.138307297866575 2 14.236392523756331 12.19307062641251 3 5.8228438430736755 7.480653484373937 4 2.9552096443844644 5.062108234190173 5 1.7290887652255238 3.702289993506093 6 1.0884633153441337 2.7967148396836286 7 0.7179145884727097 2.1520579340801174 8 0.5130506065796264 1.7576537793574634 9 0.39482997429857525 1.521723561152284 >10 1.9303449353973978 14.782102737809913 >50 0.13371725949282662 3.9582740840940134 >100 0.0884420258982591 7.253320158773245 >500 0.006345408972327438 1.918436635352333 >1k 0.005155644790016043 3.727727088192883 >5k 2.6439204051364323E-4 0.9185149435963056 >10k+ 1.3219602025682162E-4 0.637044601558658 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11253 0.635422742747242 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9107 0.5142446386029622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7118 0.40193184776280716 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3165 0.17871794017551063 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2844 0.1605920448212171 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2816 0.159010969837042 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2752 0.1553970841589274 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2710 0.1530254716826647 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2697 0.15229140115429765 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2448 0.13823112718788308 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2234 0.1261471969516874 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2103 0.11875002470429663 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.11276452654991934 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1930 0.10898123998064313 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 1868 0.1054802882299696 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 1839 0.10384274628207393 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1814 0.10243107218906042 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 1786 0.1008499972048853 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.646696372054048E-5 0.0 10-11 0.0 0.0 0.0 5.646696372054048E-5 0.0 12-13 0.0 0.0 0.0 5.646696372054048E-5 0.0 14-15 0.0 0.0 0.0 1.1293392744108096E-4 0.0 16-17 0.0 0.0 0.0 1.9763437302189167E-4 0.0 18-19 0.0 0.0 0.0 2.823348186027024E-4 0.0 20-21 0.0 0.0 0.0 3.388017823232429E-4 0.0 22-23 0.0 0.0 0.0 0.0011575727562710798 0.0 24-25 0.0 0.0 0.0 0.0020610441757997274 0.0 26-27 0.0 0.0 0.0 0.004432656652062428 0.0 28-29 0.0 0.0 0.0 0.011406326671549177 0.0 30-31 0.0 0.0 0.0 0.03546125321649942 0.0 32-33 0.0 0.0 0.0 0.08317583756035613 0.0 34-35 0.0 0.0 0.0 0.15181143196267308 0.0 36-37 0.0 0.0 0.0 0.26350308620190216 0.0 38-39 0.0 0.0 0.0 0.43219814031701687 0.0 40-41 0.0 0.0 0.0 0.621729504045011 0.0 42-43 0.0 0.0 0.0 0.8123619735655556 0.0 44-45 0.0 0.0 0.0 1.0378063262198136 0.0 46-47 0.0 0.0 0.0 1.3088195185965477 0.0 48-49 0.0 0.0 0.0 1.5868346144746286 0.0 50-51 0.0 0.0 0.0 1.9060423603868437 0.0 52-53 0.0 0.0 0.0 2.2000658404796978 0.0 54-55 0.0 0.0 0.0 2.5048462771613154 0.0 56-57 0.0 0.0 0.0 2.8533039102807707 0.0 58-59 0.0 0.0 0.0 3.2070412045080965 0.0 60-61 0.0 0.0 0.0 3.5758551780488066 0.0 62-63 0.0 0.0 0.0 3.9088973300725547 0.0 64-65 0.0 0.0 0.0 4.250381293172523 0.0 66-67 0.0 0.0 0.0 4.629754588928974 0.0 68-69 0.0 0.0 0.0 5.055487261899989 0.0 70-71 0.0 0.0 0.0 5.523005488024204 0.0 72-73 0.0 0.0 0.0 6.026662570929565 2.823348186027024E-5 74-75 0.0 0.0 0.0 6.512165524998772 8.470044558081072E-5 76-77 0.0 0.0 0.0 7.032593296129133 1.1293392744108096E-4 78-79 0.0 0.0 0.0 7.623040102272965 1.1293392744108096E-4 80-81 0.0 0.0 0.0 8.236356028723616 1.1293392744108096E-4 82-83 0.0 0.0 0.0 8.862377022011387 1.1293392744108096E-4 84-85 0.0 0.0 0.0 9.46959451638022 1.1293392744108096E-4 86-87 0.0 0.0 0.0 10.081527002219715 1.1293392744108096E-4 88-89 0.0 0.0 0.0 10.70766092943493 1.6940089116162144E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6075 0.0 42.952335 1 GTATCAA 9690 0.0 36.81836 1 TAAGGTG 1160 0.0 34.75235 5 AGGTAAG 1060 0.0 34.455387 2 AAGGTAA 1095 0.0 31.195269 1 GGTAAGG 1335 0.0 29.131027 3 GTAAGGT 1380 0.0 28.1811 4 TATCAAC 12700 0.0 28.085772 2 TCAACGC 12880 0.0 27.653318 4 ATCAACG 12945 0.0 27.514465 3 CAACGCA 13150 0.0 27.085533 5 GTACATG 9765 0.0 26.770082 1 TACATGG 9930 0.0 26.319315 2 AACGCAG 13640 0.0 26.112518 6 GGTGTGC 1740 0.0 25.34877 8 ACATGGG 10200 0.0 24.317825 3 GTGTAAG 675 0.0 23.19426 1 GTGTGCA 1975 0.0 22.812859 9 ACGCAGA 15570 0.0 22.784327 7 CGCAGAG 15550 0.0 22.722134 8 >>END_MODULE