##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939734_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1245482 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.355362823388855 31.0 31.0 34.0 30.0 34.0 2 31.602645401539323 31.0 31.0 34.0 30.0 34.0 3 31.612733865282678 31.0 31.0 34.0 30.0 34.0 4 35.45057495812866 37.0 35.0 37.0 33.0 37.0 5 35.20338953112128 37.0 35.0 37.0 32.0 37.0 6 35.23137708935175 37.0 35.0 37.0 32.0 37.0 7 35.14550270497687 37.0 35.0 37.0 32.0 37.0 8 35.11980823488417 37.0 35.0 37.0 32.0 37.0 9 36.642685321827216 39.0 35.0 39.0 32.0 39.0 10-11 36.52758409997094 38.0 35.0 39.0 32.0 39.0 12-13 36.47858178600734 38.0 35.0 39.0 32.0 39.0 14-15 37.41081043322987 39.0 36.0 41.0 32.0 41.0 16-17 37.34748755903337 39.0 36.0 41.0 32.0 41.0 18-19 37.44628585559647 39.0 36.0 41.0 32.0 41.0 20-21 37.4163584861122 39.0 36.0 41.0 32.0 41.0 22-23 37.27152178835182 39.0 36.0 40.5 31.5 41.0 24-25 37.116912568788635 39.0 36.0 40.0 31.0 41.0 26-27 36.81015261561387 39.0 35.5 40.0 30.5 41.0 28-29 36.58140462889067 38.5 35.0 40.0 30.0 41.0 30-31 36.24149967643049 38.0 35.0 40.0 30.0 41.0 32-33 36.07261405624489 38.0 35.0 40.0 30.0 41.0 34-35 35.844066795023934 38.0 35.0 40.0 30.0 41.0 36-37 35.70855018378427 38.0 35.0 40.0 29.0 41.0 38-39 35.5515667829804 38.0 34.5 40.0 28.0 41.0 40-41 35.45198124099746 38.0 34.5 40.0 27.5 41.0 42-43 35.164753083545165 38.0 34.0 40.0 26.5 41.0 44-45 34.81365085966718 38.0 34.0 40.0 25.0 41.0 46-47 34.40566664150907 38.0 33.0 40.0 24.0 41.0 48-49 34.02299912804842 38.0 33.0 40.0 23.0 41.0 50-51 33.80625773796811 38.0 33.0 40.0 21.5 41.0 52-53 33.605642233287995 38.0 33.0 40.0 20.0 41.0 54-55 33.359824148401984 38.0 33.0 40.0 18.5 41.0 56-57 33.004008488280036 37.5 32.0 40.0 15.0 41.0 58-59 32.68404400866492 37.0 31.5 40.0 12.5 41.0 60-61 32.386291813129375 37.0 31.0 40.0 8.5 41.0 62-63 32.10669483782182 36.0 31.0 39.0 8.0 41.0 64-65 31.68934235902245 36.0 31.0 39.0 4.5 40.0 66-67 30.839454122982104 35.5 29.0 39.0 2.0 40.0 68-69 29.03591581411855 34.0 25.5 38.0 2.0 40.0 70-71 28.413088266229458 34.0 24.5 37.0 2.0 39.5 72-73 28.89646939899573 34.0 26.0 37.0 2.0 39.0 74-75 28.885331542326583 34.0 26.5 36.5 2.0 39.0 76-77 28.286058730676157 32.5 26.5 35.5 2.0 38.0 78-79 28.733877326207846 34.0 28.0 36.0 2.0 38.0 80-81 28.467007150645294 34.0 27.5 35.5 2.0 37.5 82-83 27.97597275592903 33.5 26.5 35.0 2.0 37.0 84-85 27.576955748858676 33.0 26.0 35.0 2.0 36.5 86-87 27.189087437634587 33.0 26.0 35.0 2.0 36.0 88-89 26.702007736763758 33.0 25.0 35.0 2.0 35.5 90-91 26.19386711329429 32.5 24.0 35.0 2.0 35.0 92-93 25.712418565663736 32.0 22.0 35.0 2.0 35.0 94-95 24.920174679361082 32.0 18.5 34.0 2.0 35.0 96-97 24.401149113355313 31.0 13.5 34.0 2.0 35.0 98-99 23.774230779730257 31.0 2.0 34.0 2.0 35.0 100-101 22.319403652561817 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2891259244878732 1101 2 0.3213826068602508 1101 3 0.3908027546977557 1101 4 0.3616404994739284 1101 5 0.32309819461352873 1101 6 0.29258760002085893 1101 7 0.23832834398962888 1101 8 0.20564187144411505 1101 9 0.5705098650212364 1101 10-11 0.5150304251258646 1101 12-13 0.45393340121352566 1101 14-15 0.4451501659506576 1101 16-17 0.4513351897679385 1101 18-19 0.3843099540431396 1101 20-21 0.5012849254094434 1101 22-23 0.6083025529157027 1101 24-25 0.5984109245904676 1101 26-27 0.7467344636976634 1101 28-29 0.5285772758815455 1101 30-31 0.5436140750045055 1101 32-33 0.6862707729549484 1101 34-35 0.6525180821539038 1101 36-37 0.77261285212915 1101 38-39 0.7166120610808662 1101 40-41 0.8815874163652069 1101 42-43 0.8596478913894785 1101 44-45 0.6217439854235209 1101 46-47 0.571143593265738 1101 48-49 0.749283610375322 1101 50-51 0.7734962546071458 1101 52-53 0.6378079260943039 1101 54-55 0.35633097533160907 1101 56-57 0.29391747347364827 1101 58-59 0.47646185788813256 1101 60-61 0.6124890242627146 1101 62-63 0.639657488206808 1101 64-65 0.47795331067254665 1101 66-67 0.19690121237002245 1101 68-69 -8.471499733504 1101 70-71 -5.390519520360964 1101 72-73 -1.0038233453237133 1101 74-75 -0.10512149589508013 1101 76-77 0.08894719465327583 1101 78-79 0.06222120716271817 1101 80-81 -0.011084203972984596 1101 82-83 -0.04429453362474334 1101 84-85 -0.030706854639667824 1101 86-87 0.0165783480099968 1101 88-89 -0.07456881334078602 1101 90-91 -0.4355555065088801 1101 92-93 -0.6255501743833101 1101 94-95 -0.20198934633967625 1101 96-97 -0.5966718643989566 1101 98-99 -0.4082198580864649 1101 100-101 -0.3978612332262621 1102 1 0.20524233910359868 1102 2 0.23265532809753608 1102 3 0.17149008336351557 1102 4 0.11207618291602017 1102 5 0.31839419656096624 1102 6 0.17495583689987626 1102 7 0.21070255595584797 1102 8 0.15742416250278524 1102 9 0.2501469891232446 1102 10-11 0.28730733445667767 1102 12-13 0.24262727757901104 1102 14-15 0.26184327330028623 1102 16-17 0.37781222579273077 1102 18-19 0.16950487743200426 1102 20-21 0.3099403648134782 1102 22-23 0.33649590507793903 1102 24-25 0.059131987602739855 1102 26-27 0.32292235806033176 1102 28-29 0.1976452729271969 1102 30-31 0.12045860655489804 1102 32-33 -0.183669420848112 1102 34-35 0.11793346136447269 1102 36-37 -0.28231853118652595 1102 38-39 0.12068770679139362 1102 40-41 -0.16340035683597165 1102 42-43 -0.15547551075878374 1102 44-45 -0.11411359173393976 1102 46-47 0.09555915504841295 1102 48-49 0.04704073967786826 1102 50-51 -0.1973879891432162 1102 52-53 0.0884958218401053 1102 54-55 -0.1758995717484808 1102 56-57 0.04306270405392354 1102 58-59 -0.15614150973741658 1102 60-61 0.03390003004140141 1102 62-63 1.9694729544283973E-4 1102 64-65 0.0459520852264248 1102 66-67 0.511175585055021 1102 68-69 -3.584839668939864 1102 70-71 0.36464747779395523 1102 72-73 0.4562635562339601 1102 74-75 0.06610002216434196 1102 76-77 -0.25795502691734384 1102 78-79 -0.14539440109285806 1102 80-81 0.11146248331142061 1102 82-83 -0.21606273066984372 1102 84-85 -0.322368910899538 1102 86-87 -0.30530250432512673 1102 88-89 -0.3011995833801464 1102 90-91 -0.4905819794646966 1102 92-93 -0.5954036464345656 1102 94-95 -0.3274405899047004 1102 96-97 -0.5226295380983359 1102 98-99 -0.14368220812145793 1102 100-101 -0.4669810281922473 1103 1 0.04064828267349796 1103 2 0.17447285610058216 1103 3 0.14956934527453924 1103 4 0.09949963409383855 1103 5 0.2596750106486283 1103 6 0.202049155348341 1103 7 0.13610066350258165 1103 8 0.29610969645262486 1103 9 0.5455972652625576 1103 10-11 0.400569401127278 1103 12-13 0.2710402413669257 1103 14-15 0.26032101845778044 1103 16-17 0.2561814473965427 1103 18-19 0.2705144363068399 1103 20-21 0.1535419298712597 1103 22-23 0.037639508253136 1103 24-25 0.2419763298330011 1103 26-27 0.2610097243514815 1103 28-29 0.05809831314316938 1103 30-31 0.11786560745121477 1103 32-33 0.1788405754485609 1103 34-35 -0.026985333820121582 1103 36-37 0.12423286722157911 1103 38-39 0.25760879374441714 1103 40-41 0.15107709849601747 1103 42-43 0.1721903472589119 1103 44-45 0.10545875461828302 1103 46-47 0.07940004084105823 1103 48-49 0.1932751548475551 1103 50-51 0.1018615506245979 1103 52-53 0.04350108163647093 1103 54-55 0.012279706800669032 1103 56-57 -0.13273257179019282 1103 58-59 -0.2190725581006987 1103 60-61 -0.2849214535432054 1103 62-63 -0.14041471871821898 1103 64-65 -0.1692708657340276 1103 66-67 0.0733941456956444 1103 68-69 -3.5906616731369674 1103 70-71 0.30271672999045407 1103 72-73 0.24777904848112797 1103 74-75 0.07058560871214681 1103 76-77 -0.0495288868028112 1103 78-79 -0.3198089803896593 1103 80-81 -0.46296631890412954 1103 82-83 -0.39618727513390795 1103 84-85 -0.5832004686043355 1103 86-87 -0.45451702350996825 1103 88-89 -0.6032299721220369 1103 90-91 -0.6789101961253685 1103 92-93 -0.5088384008074414 1103 94-95 -0.49778956129248186 1103 96-97 -0.5219713926595979 1103 98-99 -0.3696837162875575 1103 100-101 -0.4112291993395836 1104 1 0.2304043673155718 1104 2 0.2125583834866731 1104 3 0.2437310651546305 1104 4 0.25156114197780255 1104 5 0.3282792790728095 1104 6 0.25400018815162184 1104 7 0.2503200422946392 1104 8 0.27191048314617916 1104 9 0.40179994920167417 1104 10-11 0.3434748453039518 1104 12-13 0.3480250409542833 1104 14-15 0.4021837594756974 1104 16-17 0.4460705813652339 1104 18-19 0.29921368051099506 1104 20-21 0.3582175102122491 1104 22-23 0.3499324462975508 1104 24-25 0.2969934875210427 1104 26-27 0.3074729453558689 1104 28-29 0.3230424008977124 1104 30-31 0.25859333051692346 1104 32-33 0.22708798161725952 1104 34-35 0.230792691980092 1104 36-37 0.2303305620001197 1104 38-39 0.18423272067952468 1104 40-41 0.2507723461758502 1104 42-43 0.1921521602104761 1104 44-45 0.2839084840426125 1104 46-47 0.2824906947249062 1104 48-49 0.36428925555391345 1104 50-51 0.20920524288844433 1104 52-53 0.06579598704534106 1104 54-55 0.13542379480744415 1104 56-57 0.19091685143151693 1104 58-59 0.26572639869169024 1104 60-61 -0.039915847815649386 1104 62-63 0.28502847426383937 1104 64-65 0.27125072546025564 1104 66-67 0.47010120639009756 1104 68-69 -3.307539957595168 1104 70-71 0.9157998296992602 1104 72-73 0.6322865578860117 1104 74-75 0.47751098380404855 1104 76-77 0.39674787597465766 1104 78-79 0.014723058014304513 1104 80-81 0.010564747167784105 1104 82-83 -0.1550706823064658 1104 84-85 -0.06801865764536075 1104 86-87 -0.0025639098404397487 1104 88-89 0.11927043459272824 1104 90-91 0.005467164554328718 1104 92-93 -0.3682736789309793 1104 94-95 0.10325036737794235 1104 96-97 -0.2693867045562257 1104 98-99 0.14480289617569397 1104 100-101 0.3979704995023212 1105 1 0.10275324026639154 1105 2 0.22024098009088888 1105 3 0.1037860758884328 1105 4 0.10758853756718878 1105 5 0.12949780951777257 1105 6 0.1262691674646561 1105 7 0.06003291309870207 1105 8 0.11442426632338254 1105 9 0.17138608796045673 1105 10-11 0.3172154532274476 1105 12-13 0.05573203256239623 1105 14-15 0.24621308227038696 1105 16-17 0.24125439771965063 1105 18-19 0.20100274910974036 1105 20-21 0.3205741895643115 1105 22-23 0.2918543456925491 1105 24-25 0.1633704635163653 1105 26-27 0.21735627624970277 1105 28-29 0.22768446520294816 1105 30-31 0.4426100288971 1105 32-33 0.4566532016601954 1105 34-35 0.4742496469957018 1105 36-37 0.3557239314380567 1105 38-39 0.4957941229043499 1105 40-41 0.30605172995804253 1105 42-43 0.5459818708857327 1105 44-45 0.4332467695617481 1105 46-47 0.5346359303806381 1105 48-49 0.5556552537974682 1105 50-51 0.7385676794614326 1105 52-53 0.7319823770726614 1105 54-55 0.5510859971883946 1105 56-57 0.5411043880240172 1105 58-59 0.6008884425051164 1105 60-61 0.408043510107845 1105 62-63 0.4736604024449491 1105 64-65 0.5708625399606433 1105 66-67 1.1785473666164847 1105 68-69 2.431640553236214 1105 70-71 2.61598157247834 1105 72-73 1.4990739798220005 1105 74-75 0.9340467102872658 1105 76-77 0.949636854311894 1105 78-79 0.8379852189237518 1105 80-81 0.8715071261685665 1105 82-83 0.629949260609223 1105 84-85 0.5960566497316933 1105 86-87 0.41006294417985956 1105 88-89 0.6550633941946025 1105 90-91 0.778663763890787 1105 92-93 0.5007303340471658 1105 94-95 0.6962084445637373 1105 96-97 0.5416380075827263 1105 98-99 0.8138538695363486 1105 100-101 0.6032068870901419 1106 1 0.48796415869600906 1106 2 0.4309095876987037 1106 3 0.4750260321536892 1106 4 0.27530682987800503 1106 5 0.48621694092542356 1106 6 0.4616676113214524 1106 7 0.5021415888513445 1106 8 0.49462603096943525 1106 9 0.6008669921508556 1106 10-11 0.5991093964739846 1106 12-13 0.5620017386242395 1106 14-15 0.654495985036867 1106 16-17 0.709646510209474 1106 18-19 0.7911424078770395 1106 20-21 0.7715296157237859 1106 22-23 0.691935394817861 1106 24-25 0.6627799173406075 1106 26-27 0.7060364150820817 1106 28-29 0.8669608713258086 1106 30-31 0.8909378741981513 1106 32-33 0.7135493697846798 1106 34-35 0.9549545518980622 1106 36-37 0.9066386741150296 1106 38-39 0.8697713147269681 1106 40-41 0.893079737993034 1106 42-43 0.792563504836103 1106 44-45 0.7944506192362724 1106 46-47 0.8669389333301964 1106 48-49 0.9914827812588243 1106 50-51 0.9023409453279854 1106 52-53 0.7438807065071629 1106 54-55 0.8595704103291553 1106 56-57 0.878453414163161 1106 58-59 0.8364226039168301 1106 60-61 0.9390710223128877 1106 62-63 0.7816445358867696 1106 64-65 0.7038482626009994 1106 66-67 1.309138167064006 1106 68-69 2.7789977160718564 1106 70-71 2.8975710252854974 1106 72-73 1.793288025794336 1106 74-75 1.306248131666436 1106 76-77 0.7000761087870764 1106 78-79 0.5747958245990965 1106 80-81 0.7480136863801192 1106 82-83 0.9026407908167116 1106 84-85 0.944880007136085 1106 86-87 0.9530450434475561 1106 88-89 1.1090014281653104 1106 90-91 0.9497028454611716 1106 92-93 0.987224371374257 1106 94-95 1.6348123018664218 1106 96-97 1.2186562897226771 1106 98-99 1.3913714778561221 1106 100-101 1.545679171467686 1107 1 0.24245873702459164 1107 2 0.14237401308097475 1107 3 0.20092983542313902 1107 4 0.12306992105307302 1107 5 0.11028418442242582 1107 6 0.13795990926480783 1107 7 0.17406204162654149 1107 8 0.06479433211285368 1107 9 0.0371106064577873 1107 10-11 0.17403217233499646 1107 12-13 0.1592771555601118 1107 14-15 0.3097154182734343 1107 16-17 0.09324178807052164 1107 18-19 0.25100602666504557 1107 20-21 0.10383422484272842 1107 22-23 0.11171662877799093 1107 24-25 0.31196230657509716 1107 26-27 0.2544447940796033 1107 28-29 0.15007880873841373 1107 30-31 0.24773046415644018 1107 32-33 0.31838893535584845 1107 34-35 0.4655393948027182 1107 36-37 0.23864071728855407 1107 38-39 0.23613367763356052 1107 40-41 0.4554036678871114 1107 42-43 0.37898595889107156 1107 44-45 0.4985612956868408 1107 46-47 0.45153947490771174 1107 48-49 0.5561623809904575 1107 50-51 0.3808165591334429 1107 52-53 0.522273960310649 1107 54-55 0.6022975008115807 1107 56-57 0.6095275263726094 1107 58-59 0.640842735506638 1107 60-61 0.5724873983610905 1107 62-63 0.7202183734594385 1107 64-65 0.7135896447470671 1107 66-67 1.2318612729856682 1107 68-69 2.5082254002041786 1107 70-71 2.5538784546293662 1107 72-73 1.7946534515992703 1107 74-75 1.3352746016869617 1107 76-77 0.7163994639610145 1107 78-79 0.631605606509563 1107 80-81 0.5882250418817634 1107 82-83 0.6067402956015968 1107 84-85 0.7191729493121635 1107 86-87 0.5959325093972474 1107 88-89 0.7032142570855235 1107 90-91 0.6448650177046709 1107 92-93 0.8279474512287059 1107 94-95 1.2011983735301754 1107 96-97 0.8535355333264327 1107 98-99 1.1798468496088113 1107 100-101 1.2148451880054765 1108 1 -0.16727772281700837 1108 2 -0.19117292101852001 1108 3 -0.20051587992641018 1108 4 -0.1253261764725977 1108 5 -0.38828743340494754 1108 6 -0.2349911137136118 1108 7 -0.35353943770466145 1108 8 -0.05774422545167823 1108 9 0.01631663023283636 1108 10-11 -0.1849011544619188 1108 12-13 -0.2269471707658326 1108 14-15 -0.1695804812338224 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-0.19291306534389108 2316 100-101 -0.4202493254847077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 21.0 5 108.0 6 243.0 7 528.0 8 1104.0 9 1955.0 10 3276.0 11 5146.0 12 7028.0 13 8846.0 14 10677.0 15 13259.0 16 16383.0 17 18939.0 18 16181.0 19 12409.0 20 11778.0 21 12090.0 22 12579.0 23 13896.0 24 15856.0 25 17990.0 26 21186.0 27 25852.0 28 31369.0 29 37519.0 30 44400.0 31 52636.0 32 62122.0 33 75627.0 34 93556.0 35 118958.0 36 148816.0 37 188721.0 38 132864.0 39 11559.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.01599335049249 26.05209583964086 16.07189177685602 20.86001903301063 2 13.91444996088875 24.777178303936985 40.81415650604016 20.49421522913411 3 15.328616012608784 31.540103783197015 33.889878689438056 19.241401514756145 4 10.145228915391792 21.556313138206733 42.85537647272301 25.443081473678465 5 9.28588289513618 42.002774829343174 37.36007425237779 11.351268023142849 6 22.97877123679224 44.7923692070527 19.451697337572664 12.777162218582394 7 22.06816316895788 37.55983627222232 25.3467332325959 15.025267326223904 8 23.026266136323127 37.08243073765819 22.596633271295772 17.294669854722912 9 24.91107860250088 17.55834287448554 24.810394690569595 32.720183832443986 10-11 20.578820087323624 30.00609402624847 31.239391657205807 18.1756942292221 12-13 22.09461879015514 29.5476771241977 30.405537775736626 17.95216630991054 14-15 20.48343532865188 30.31962726077133 28.94377437811225 20.253163032464542 16-17 17.057982132971382 33.277370906041334 30.37077717519813 19.293869785789155 18-19 16.85821909036786 31.50873778534647 34.30865894033753 17.324384183948137 20-21 17.29149839178728 31.129032776065813 34.16171409944102 17.41775473270589 22-23 17.690219529467306 31.00442238426569 33.60686866610678 17.698489420160225 24-25 17.515562260684828 32.258380497173384 33.47032190776093 16.75573533438085 26-27 16.9516844135344 31.500797284255523 34.49362413466958 17.053894167540495 28-29 16.609091832429332 30.93103424742499 35.15289882097712 17.306975099168554 30-31 17.3301613198488 30.89304811975502 34.35879133760908 17.417999222787113 32-33 16.746777983459047 31.730023055840256 34.32770928203318 17.195489678667506 34-35 17.511823225713208 31.26827682005412 34.19963386139727 17.020266092835406 36-37 17.69414608995202 30.568954548980038 33.62890822952044 18.107991131547507 38-39 16.48364321678715 31.798784040633777 33.81524329233254 17.902329450246526 40-41 16.30623357093398 31.102148452648727 35.03656440475191 17.55505357166539 42-43 16.770859538952568 30.714445890302507 34.31380192530424 18.200892645440682 44-45 16.653064111234748 30.67157242727857 34.21635416955495 18.459009291931736 46-47 17.600404398232605 30.93763009134321 33.06349341567027 18.398472094753913 48-49 16.77839592864275 31.027262261387406 33.26036072418081 18.933981085789025 50-51 16.226546408115603 30.720177060705495 33.31396530953879 19.73931122164011 52-53 16.668802595575453 31.414746908493996 33.27609847342014 18.640352022510413 54-55 17.19453414632089 30.71423492790888 32.595718117359915 19.495512808410314 56-57 17.069819928356868 31.156691243795482 32.22334666602413 19.550142161823526 58-59 16.613527437067052 30.266916724182302 33.0344527703265 20.085103068424136 60-61 17.306563499462403 31.47061445031425 31.811882054703993 19.410939995519357 62-63 16.620739713004713 30.92550037897766 32.606330532752665 19.847429375264962 64-65 16.579928336996776 30.614635713066956 32.32724097437596 20.478194975560307 66-67 16.557705040213897 30.845124947086983 31.41958045050336 21.177589562195763 68-69 16.814889716328207 30.184505354689488 31.597806457385154 21.402798471597155 70-71 17.15341673322056 30.22547465485325 31.72912256044047 20.891986051485716 72-73 17.29261540131853 29.50312944208873 31.599825483088146 21.604429673504594 74-75 16.507396361191883 30.40621858950908 30.928182390970083 22.158202658328953 76-77 16.50618578890895 30.168330238564884 31.34288321050636 21.982600762019803 78-79 16.83529083503791 29.802817919220185 30.796175595028995 22.56571565071291 80-81 16.75653280886995 29.691114994538175 30.547743872060483 23.00460832453139 82-83 16.914556269648543 29.871643915741757 30.485463240893772 22.728336573715925 84-85 16.410580042826965 29.265413973923245 30.42653537560308 23.89747060764671 86-87 16.254464199687828 29.366725975218877 30.886802027196293 23.492007797897006 88-89 17.142360489418127 28.845488362308796 30.463139081429713 23.549012066843357 90-91 16.8546001689314 28.51520221987417 30.840899383047127 23.78929822814731 92-93 17.268050238970332 28.512562753548426 30.536895372948276 23.68249163453297 94-95 16.799724884457902 27.471821547717763 30.955600658220362 24.772852909603976 96-97 17.187351312067488 27.232020429345503 30.736889865029 24.84373839355801 98-99 16.83494585834835 27.45239517784352 30.506074272516727 25.2065846912914 100-101 16.4593426685676 27.30798925766861 30.944868264312102 25.287799809451695 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4331.0 1 2724.5 2 1322.5 3 1569.0 4 1359.0 5 1288.5 6 2157.0 7 3974.5 8 5701.0 9 5973.0 10 4743.0 11 3467.0 12 3033.5 13 2889.0 14 2790.0 15 2707.5 16 2671.5 17 2728.0 18 2803.5 19 3057.0 20 3405.5 21 3692.0 22 4039.0 23 4631.5 24 5157.0 25 6496.0 26 10492.0 27 14906.0 28 16007.5 29 17669.5 30 22487.5 31 27282.0 32 34544.0 33 44773.0 34 56001.0 35 64040.0 36 71281.0 37 78486.5 38 76748.5 39 71132.5 40 65181.0 41 58511.0 42 52910.5 43 50164.5 44 49055.5 45 47677.0 46 43855.0 47 37869.5 48 33968.5 49 28815.0 50 22119.0 51 15943.0 52 11065.0 53 7453.5 54 4944.5 55 3654.0 56 2913.5 57 2478.0 58 2171.5 59 1937.0 60 1728.0 61 1528.5 62 1242.0 63 937.0 64 717.0 65 602.5 66 517.0 67 452.0 68 408.0 69 380.5 70 346.0 71 288.5 72 249.5 73 243.5 74 209.0 75 150.5 76 105.5 77 76.0 78 52.5 79 35.5 80 37.5 81 36.0 82 25.0 83 14.5 84 8.0 85 4.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022240385649892974 2 0.02537170348507646 3 8.02902009021407E-5 4 0.0 5 0.0 6 1.605804018042814E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8101570315749243E-4 18-19 4.014510045107035E-5 20-21 0.0 22-23 0.0 24-25 8.02902009021407E-5 26-27 3.211608036085628E-4 28-29 1.2043530135321106E-4 30-31 4.817412054128442E-4 32-33 0.002207980524808869 34-35 0.004175090446911316 36-37 0.1040962454696254 38-39 0.09610737047986243 40-41 0.10497943767954897 42-43 0.10558161418631501 44-45 0.09879709221008413 46-47 0.09345779385009177 48-49 0.09470229196407495 50-51 0.09337750364918963 52-53 0.07246190631418198 54-55 0.062024180196903686 56-57 0.03388246478070338 58-59 0.013528898852010706 60-61 0.010036275112767588 62-63 0.002087545223455658 64-65 0.005580168962698778 66-67 0.13613203562957954 68-69 0.38864471746681206 70-71 0.37198450077961787 72-73 0.16455476674893738 74-75 0.03022926063965597 76-77 0.029707374333792058 78-79 0.004777266953677372 80-81 0.0014853687166896028 82-83 1.2043530135321106E-4 84-85 4.014510045107035E-4 86-87 0.0014452236162385325 88-89 2.408706027064221E-4 90-91 4.817412054128442E-4 92-93 0.002770011931123854 94-95 0.07338524362455659 96-97 0.017222248093509182 98-99 0.018466746207492363 100-101 0.010317290815925079 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1245482.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.77666429315785 #Duplication Level Percentage of deduplicated Percentage of total 1 75.12379813115072 14.105743379355761 2 9.817419573718716 3.686767831215864 3 3.9220864727200135 2.2093110308099786 4 2.0924549424069134 1.571572960085342 5 1.2934630767305262 1.2143460983682082 6 0.892874706755574 1.0059125174760704 7 0.6707671305474243 0.881633846042164 8 0.5315849955133688 0.7985114403227477 9 0.42843074556853455 0.7240050254166929 >10 3.573032080025785 14.387348453860291 >50 0.7558500724600845 10.143002633408805 >100 0.8001864555762507 30.596706478628562 >500 0.07247293417579255 9.338769858347714 >1k 0.023873437140261074 6.380951084790667 >5k 0.0012789341325139861 1.9368986188130186 >10k+ 4.2631137750466204E-4 1.0185187430581002 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12724 1.0216125162788383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9737 0.781785686184144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8121 0.6520367215262846 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6339 0.5089595835186699 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 3283 0.2635927295617279 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2750 0.2207980524808869 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2365 0.18988632513356274 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2182 0.17519321836847102 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2074 0.16652187667103982 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2032 0.1631496882331499 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1912 0.153514864124893 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1899 0.15247109151316518 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1693 0.1359313101273242 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1670 0.13408463550657498 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1636 0.13135476867590218 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1611 0.12934751365334868 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1580 0.1268585174253823 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1557 0.12501184280463307 RNA PCR Primer, Index 31 (95% over 22bp) ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1551 0.12453010159922022 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1536 0.12332574858568812 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1518 0.1218805249694496 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1499 0.1203550111523089 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1475 0.11842804633065752 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1467 0.1177857247234404 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 1455 0.11682224231261472 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1451 0.11650108150900616 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1406 0.11288802246840982 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1400 0.11240628126299697 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 1378 0.11063989684314988 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1377 0.11055960664224775 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1368 0.10983699483412847 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1364 0.10951583403051991 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1328 0.10662538679804284 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1260 0.10116565313669729 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1251 0.10044304132857801 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1248 0.10020217072587159 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.211608036085628E-4 0.0 18-19 0.0 0.0 0.0 4.8174120541284417E-4 0.0 20-21 0.0 0.0 0.0 8.430471094724773E-4 0.0 22-23 0.0 0.0 0.0 0.002970737433379206 0.0 24-25 0.0 0.0 0.0 0.007908584788860858 0.0 26-27 0.0 0.0 0.0 0.01726239319396025 0.0 28-29 0.0 0.0 0.0 0.04098814756054282 0.0 30-31 0.0 0.0 0.0 0.11890978753607037 0.0 32-33 0.0 0.0 0.0 0.2745523419848701 0.0 34-35 0.0 0.0 0.0 0.5065107323911546 0.0 36-37 0.0 0.0 0.0 0.8535651257906578 0.0 38-39 0.0 0.0 0.0 1.3538533676118965 0.0 40-41 0.0 0.0 0.0 1.9113483775759104 0.0 42-43 0.0 0.0 0.0 2.4355630992659867 0.0 44-45 0.0 0.0 0.0 3.024009981677776 0.0 46-47 0.0 0.0 0.0 3.7267499650737625 0.0 48-49 0.0 0.0 0.0 4.428687046460728 0.0 50-51 0.0 0.0 0.0 5.1591271491679525 0.0 52-53 0.0 0.0 0.0 5.837940652695101 0.0 54-55 0.0 0.0 0.0 6.513542548186164 0.0 56-57 0.0 0.0 0.0 7.314638027687273 0.0 58-59 0.0 8.02902009021407E-5 0.0 8.102124318135468 0.0 60-61 0.0 1.605804018042814E-4 0.0 8.868935881851364 0.0 62-63 0.0 1.605804018042814E-4 0.0 9.569588320023895 0.0 64-65 0.0 1.605804018042814E-4 0.0 10.31953894155034 0.0 66-67 0.0 1.605804018042814E-4 0.0 11.084543975745936 0.0 68-69 0.0 1.605804018042814E-4 0.0 11.914503782471364 0.0 70-71 0.0 1.605804018042814E-4 0.0 12.894204813879284 0.0 72-73 0.0 1.605804018042814E-4 0.0 13.79526159350356 0.0 74-75 0.0 1.605804018042814E-4 0.0 14.716150052750661 0.0 76-77 0.0 1.605804018042814E-4 0.0 15.684008279525518 0.0 78-79 0.0 1.605804018042814E-4 0.0 16.72195985168794 0.0 80-81 0.0 1.605804018042814E-4 0.0 17.824424600275236 0.0 82-83 0.0 1.605804018042814E-4 0.0 18.847522485270762 0.0 84-85 0.0 1.605804018042814E-4 0.0 19.807391837055853 0.0 86-87 0.0 1.605804018042814E-4 0.0 20.82221983135846 0.0 88-89 0.0 1.605804018042814E-4 0.0 21.845518441856246 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATAGT 20 5.2399555E-4 47.771694 68-69 GGACTGA 60 8.352233E-5 39.534054 6 GTATGGT 130 2.0736479E-10 36.51203 1 CTTTAGG 390 0.0 35.285038 2 CATTCCG 435 0.0 34.899025 9 GGGACAT 55 0.0025955145 34.50245 7 TGGGCGC 55 0.0025955145 34.50245 6 CCTTTAG 400 0.0 34.412586 1 TAGGCAT 430 0.0 34.201553 5 ACTGAGT 85 1.6462875E-5 33.487667 8 GTGGTAC 285 0.0 33.30922 1 GGCATTC 475 0.0 32.95892 7 TTAGGCA 480 0.0 32.615593 4 CATGGGG 1875 0.0 32.386295 4 GGTATCA 6760 0.0 32.228886 1 GACTGAC 60 0.0039752447 31.627241 7 CATATAG 425 0.0 31.25516 3 GGTACTC 310 0.0 30.60701 3 GTACATG 6780 0.0 29.753534 1 GACATAT 470 0.0 29.287308 1 >>END_MODULE