FastQCFastQC Report
Fri 27 May 2016
SRR939733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827713
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128800.7047058263523869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90960.49767113326873536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65610.358973208594566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26410.1444975223134048No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18790.10280607513324029No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.10089111364858705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60750.042.7211231
GTATCAA109250.041.0838661
TATCAAC142400.032.519322
ATCAACG142850.032.253463
TCAACGC144250.031.8746384
CAACGCA147450.031.0863465
AACGCAG152400.030.232336
GTACATG124550.027.8467981
TACATGG127850.027.0166992
ACGCAGA171800.026.7079567
CGCAGAG172850.026.5175388
ACATGGG131750.025.138663
GCAGAGT178200.024.5764689
TACGCGC500.001594583623.9005688-89
GAGTACT93800.023.01527412-13
CATGGGG39250.022.8485934
AGTACTT97050.022.09787212-13
CATGGGA86150.021.9763184
GTACTTT102650.020.84754414-15
AGAGTAC168050.020.69451110-11
Produced by FastQC (version 0.11.5)