##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939733_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1827713 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.159218651943714 31.0 30.0 33.0 22.0 34.0 2 29.982206725016454 31.0 30.0 33.0 25.0 34.0 3 30.25029039023085 31.0 30.0 34.0 26.0 34.0 4 34.04889772081284 35.0 35.0 37.0 30.0 37.0 5 34.03865431826551 35.0 35.0 37.0 30.0 37.0 6 34.10171618848255 36.0 35.0 37.0 30.0 37.0 7 34.02299376324401 36.0 35.0 37.0 29.0 37.0 8 33.90036181829423 36.0 35.0 37.0 29.0 37.0 9 35.19156891700174 38.0 35.0 39.0 28.0 39.0 10-11 35.02984057124942 37.0 34.5 39.0 28.0 39.0 12-13 34.91078741574853 37.0 34.0 39.0 27.0 39.0 14-15 35.57101333743317 38.0 34.0 40.0 27.0 41.0 16-17 35.42733897499224 38.0 34.0 40.0 27.0 41.0 18-19 35.35922789847203 38.0 34.0 40.0 27.0 41.0 20-21 35.58560014619363 38.0 34.0 40.0 27.0 41.0 22-23 35.481972005451624 38.0 34.0 40.0 27.0 41.0 24-25 35.27657460443734 38.0 34.0 40.0 26.5 41.0 26-27 35.16681721911482 38.0 34.0 40.0 25.5 41.0 28-29 35.19032665413005 38.0 34.0 40.0 26.0 41.0 30-31 34.93736051557329 38.0 34.0 40.0 25.0 41.0 32-33 34.562284942986125 38.0 33.5 40.0 24.0 41.0 34-35 34.34936447899643 38.0 33.0 40.0 24.0 41.0 36-37 34.111311239784364 38.0 33.0 40.0 23.5 41.0 38-39 33.87152496042869 38.0 33.0 40.0 22.0 41.0 40-41 33.60973440578472 38.0 33.0 40.0 20.5 41.0 42-43 33.173625180758684 37.0 32.0 40.0 18.0 41.0 44-45 32.75249369020191 37.0 31.0 40.0 15.0 41.0 46-47 32.27477563490548 37.0 30.5 40.0 10.5 41.0 48-49 31.847732931811507 37.0 30.0 39.5 8.5 41.0 50-51 31.208288719290174 35.5 29.5 38.5 7.5 40.5 52-53 31.345505831604854 36.0 30.0 39.0 6.0 40.0 54-55 31.43937888497811 36.0 30.0 39.0 2.0 40.0 56-57 31.151896386358253 36.0 29.5 39.0 2.0 40.0 58-59 30.78054979091356 35.5 29.0 39.0 2.0 40.0 60-61 30.38120892065658 35.0 28.0 39.0 2.0 40.0 62-63 29.944836525209375 35.0 28.0 38.5 2.0 40.0 64-65 29.412542067600327 34.5 26.5 38.0 2.0 40.0 66-67 28.83076746732118 34.0 26.0 38.0 2.0 40.0 68-69 28.274604382635566 34.0 26.0 37.0 2.0 39.0 70-71 27.706497409604246 33.0 25.0 36.5 2.0 39.0 72-73 27.057815970012797 33.0 23.5 36.0 2.0 39.0 74-75 26.692558678523376 32.5 22.5 35.5 2.0 38.0 76-77 26.11728756101204 32.0 20.5 35.0 2.0 37.5 78-79 25.446704159788766 31.5 19.0 35.0 2.0 37.0 80-81 24.70795332746443 31.0 15.0 34.5 2.0 36.0 82-83 23.948728547643967 30.5 7.5 34.0 2.0 36.0 84-85 23.142429090344052 30.0 2.0 34.0 2.0 35.0 86-87 22.159443796701126 29.0 2.0 34.0 2.0 35.0 88-89 21.12666649523202 29.0 2.0 34.0 2.0 35.0 90-91 19.68114550807485 26.0 2.0 33.5 2.0 35.0 92-93 18.090622543036023 22.0 2.0 32.0 2.0 34.0 94-95 17.618849622451663 21.0 2.0 32.0 2.0 34.0 96-97 17.37754778786385 21.5 2.0 32.0 2.0 34.0 98-99 16.538915026593344 13.0 2.0 32.0 2.0 34.0 100-101 14.914566729021459 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5986479600032126 1101 2 0.9807556648313884 1101 3 0.9499151434575808 1101 4 0.7401827479431375 1101 5 0.8395847590828609 1101 6 0.8022560954051698 1101 7 0.7394015588436815 1101 8 0.893065181995695 1101 9 0.7831150633303423 1101 10-11 0.84895836073796 1101 12-13 0.9855953382012572 1101 14-15 1.1959956292950977 1101 16-17 1.1009954735088385 1101 18-19 1.119975394840239 1101 20-21 1.2504465375097311 1101 22-23 1.1953651219058798 1101 24-25 1.2066219252060577 1101 26-27 0.9656343828284193 1101 28-29 1.1956969027612345 1101 30-31 1.18786588547934 1101 32-33 1.236429622859795 1101 34-35 1.2153073333348203 1101 36-37 1.2039871478250888 1101 38-39 1.1777562206062129 1101 40-41 1.2982044793564356 1101 42-43 1.1004829755593946 1101 44-45 0.9982264931621856 1101 46-47 0.8466966504743993 1101 48-49 1.1443425899299164 1101 50-51 1.130051033025378 1101 52-53 1.0832835931489484 1101 54-55 0.8795399160287793 1101 56-57 1.0471018025110936 1101 58-59 0.9134550959880912 1101 60-61 0.871484000412579 1101 62-63 0.47764115585885847 1101 64-65 0.6636028628300785 1101 66-67 0.8709116255218312 1101 68-69 0.7303274604930436 1101 70-71 0.563987304761433 1101 72-73 0.46727741796501476 1101 74-75 0.2741068499644719 1101 76-77 0.3891447837245394 1101 78-79 0.18176719191170676 1101 80-81 0.15818522422683756 1101 82-83 0.3292681560532422 1101 84-85 0.42040177333788975 1101 86-87 0.056474238162078194 1101 88-89 -4.169561748688739 1101 90-91 -7.833483912915664 1101 92-93 -10.04432576472667 1101 94-95 -7.901583650023422 1101 96-97 -4.476661524870657 1101 98-99 -2.906003317730079 1101 100-101 -2.1569973161474234 1102 1 1.7033769786825061 1102 2 0.8984681089250479 1102 3 0.9095737859200916 1102 4 0.886180511689524 1102 5 0.838861265265443 1102 6 0.7514964268968782 1102 7 0.8335313930978216 1102 8 0.8683447136978728 1102 9 1.26510006727667 1102 10-11 1.2058529279571175 1102 12-13 1.0425622548021494 1102 14-15 1.3262228721259248 1102 16-17 1.3147681649058427 1102 18-19 1.3194442846060923 1102 20-21 1.310276187601815 1102 22-23 1.321509557580697 1102 24-25 1.2697697810334674 1102 26-27 1.3815693341570139 1102 28-29 1.2873224618246368 1102 30-31 1.5580595845059122 1102 32-33 1.4884455397237772 1102 34-35 1.6631335632743003 1102 36-37 1.6459103259361143 1102 38-39 1.467041934891931 1102 40-41 1.3716210144787695 1102 42-43 1.4561574691137125 1102 44-45 1.1736655223650345 1102 46-47 1.3905831277172283 1102 48-49 1.309147904320863 1102 50-51 1.5196869840908924 1102 52-53 1.452360809670921 1102 54-55 1.4994294187912125 1102 56-57 1.6846100240317412 1102 58-59 1.4474445066694948 1102 60-61 1.3900679256954014 1102 62-63 1.3945643339698783 1102 64-65 1.47265968998083 1102 66-67 1.2801098628278034 1102 68-69 1.3532655031134055 1102 70-71 1.174269138489393 1102 72-73 1.0268604224375224 1102 74-75 0.8663309357313764 1102 76-77 0.8470621081128584 1102 78-79 0.9191340032771329 1102 80-81 0.709510533356017 1102 82-83 0.5641438466609792 1102 84-85 0.6725607446612258 1102 86-87 0.5931915493959679 1102 88-89 -0.15692362714177577 1102 90-91 -5.565021666138499 1102 92-93 -10.546976382985024 1102 94-95 -8.290408959152597 1102 96-97 -4.663350882934324 1102 98-99 -3.03428206016628 1102 100-101 -2.2133015781837297 1103 1 1.956327710910223 1103 2 1.1707525624213133 1103 3 1.0103120751257926 1103 4 0.8398404379818345 1103 5 0.9031130352198602 1103 6 0.7759908988200692 1103 7 0.7663543491641605 1103 8 0.7113849134845083 1103 9 0.9764980888070767 1103 10-11 0.8105111812788053 1103 12-13 0.7042372863218844 1103 14-15 0.8035353296955137 1103 16-17 0.7589855056099495 1103 18-19 0.7960960400129409 1103 20-21 0.617092936704708 1103 22-23 0.7457312625559709 1103 24-25 0.6235763953288895 1103 26-27 0.6729177727177813 1103 28-29 0.6057730243306949 1103 30-31 0.7138598221162269 1103 32-33 0.7208878343605747 1103 34-35 0.648664792059094 1103 36-37 0.6070804355278128 1103 38-39 0.5444641053894586 1103 40-41 0.4093846891950932 1103 42-43 0.4257791110495148 1103 44-45 0.37832256338918313 1103 46-47 0.4425482620398 1103 48-49 0.34500281655052945 1103 50-51 0.3426121368403834 1103 52-53 0.17273346993665228 1103 54-55 0.1919228723844597 1103 56-57 0.2213202121803235 1103 58-59 0.2355342894257717 1103 60-61 0.15526892462857944 1103 62-63 0.2918487874661473 1103 64-65 0.17543100217169538 1103 66-67 0.14754633843637777 1103 68-69 0.026596169392135494 1103 70-71 0.16397733846999785 1103 72-73 0.3859575032250291 1103 74-75 -0.016729024505263368 1103 76-77 -0.005932872975296988 1103 78-79 -0.25799903425559734 1103 80-81 -0.3742677131768133 1103 82-83 -0.8626345938394593 1103 84-85 -0.7534181856074191 1103 86-87 -0.7934040766344879 1103 88-89 -1.4889028241158861 1103 90-91 -6.969642944385033 1103 92-93 -10.188068420614739 1103 94-95 -8.673829577909267 1103 96-97 -5.418329182808247 1103 98-99 -3.8985326178231503 1103 100-101 -3.115552814727227 1104 1 1.9597192716972742 1104 2 1.2198427563449918 1104 3 0.9108368521221806 1104 4 0.7586191212797004 1104 5 0.7189425660784465 1104 6 0.8933269395897625 1104 7 0.9397596160943351 1104 8 0.9887176189575442 1104 9 1.01760131167687 1104 10-11 0.928804852629419 1104 12-13 0.8518216292836485 1104 14-15 0.9839993363701609 1104 16-17 0.9053853874045998 1104 18-19 0.8993109263847572 1104 20-21 0.9063210689689924 1104 22-23 1.0010640623094176 1104 24-25 0.9131786079788853 1104 26-27 0.6933486190417071 1104 28-29 0.7244377347630788 1104 30-31 0.8113997620403381 1104 32-33 1.061593457427442 1104 34-35 0.9155705136642212 1104 36-37 0.9845945801255951 1104 38-39 0.808667121551963 1104 40-41 1.0417274106957919 1104 42-43 1.1200771223408665 1104 44-45 0.7379543476512467 1104 46-47 0.6660207417872499 1104 48-49 0.45648903589544787 1104 50-51 0.5103259835931304 1104 52-53 0.45787142855261465 1104 54-55 0.3414901455209254 1104 56-57 0.35902411893799524 1104 58-59 0.20694653918981842 1104 60-61 0.17707431361642278 1104 62-63 0.1268399271339753 1104 64-65 0.3492027462277214 1104 66-67 0.17403926385651047 1104 68-69 0.07497406458677602 1104 70-71 0.04258774642037366 1104 72-73 0.04472586129599421 1104 74-75 -0.15083038001443327 1104 76-77 0.09736387516113965 1104 78-79 0.1805420421024202 1104 80-81 0.14648373727172626 1104 82-83 0.06122572819407779 1104 84-85 -0.05774765918812008 1104 86-87 -0.05301510399709741 1104 88-89 -0.37411501589240004 1104 90-91 -3.0200531909435426 1104 92-93 -8.168508654430187 1104 94-95 -5.975496896766668 1104 96-97 -3.3213700052173856 1104 98-99 -2.4533359012148956 1104 100-101 -2.008666187442069 1105 1 1.8059930868541691 1105 2 1.204332306455914 1105 3 0.8628145266608307 1105 4 0.7335129631886375 1105 5 0.6416243458062993 1105 6 0.6903191782196316 1105 7 0.5476480891589617 1105 8 0.5860622328042808 1105 9 0.6587508609274622 1105 10-11 0.6144030455350062 1105 12-13 0.6487681630913897 1105 14-15 0.8178123782987683 1105 16-17 0.5108733971221895 1105 18-19 0.6921331441005023 1105 20-21 0.7445942358087478 1105 22-23 0.6537185522896891 1105 24-25 0.5682927087230709 1105 26-27 0.5092699514409702 1105 28-29 0.4935136728769578 1105 30-31 0.8116346873865652 1105 32-33 0.9038776384892131 1105 34-35 0.7554689542208024 1105 36-37 1.0135617074763843 1105 38-39 0.8321506944451329 1105 40-41 0.68724785163338 1105 42-43 1.119344564939702 1105 44-45 0.8506018833291691 1105 46-47 0.7267089360652577 1105 48-49 0.5047019901527356 1105 50-51 0.6037147618686696 1105 52-53 0.5473123087890848 1105 54-55 0.6034785075625244 1105 56-57 0.6619873021034621 1105 58-59 0.6639311321838939 1105 60-61 0.7588509944784718 1105 62-63 0.7258914944637063 1105 64-65 0.6172217123206316 1105 66-67 0.6194029257319791 1105 68-69 0.6610403414438011 1105 70-71 0.6379706376075625 1105 72-73 0.6632287351947248 1105 74-75 0.6702073319688857 1105 76-77 0.5411868876543053 1105 78-79 0.21764590803904227 1105 80-81 0.05278654746536304 1105 82-83 -0.062259739464831654 1105 84-85 0.1488504272009088 1105 86-87 0.5159943124765043 1105 88-89 0.45060135816098423 1105 90-91 -2.578205087655256 1105 92-93 -7.7460063653483395 1105 94-95 -5.121434826289576 1105 96-97 -2.3741570263793577 1105 98-99 -1.496789408784739 1105 100-101 -1.108062013221943 1106 1 1.5641262784023944 1106 2 0.9989232909978796 1106 3 0.6310023573486632 1106 4 0.518358382837981 1106 5 0.6116203689068982 1106 6 0.5608451663926743 1106 7 0.5607372754507622 1106 8 -0.35472951599400204 1106 9 -0.5120007730594622 1106 10-11 -0.5575049151801394 1106 12-13 -0.2899832634051336 1106 14-15 0.3706381382323727 1106 16-17 0.43729967751088594 1106 18-19 0.5225605311046877 1106 20-21 0.26708641169145153 1106 22-23 0.2564360281689346 1106 24-25 0.3284532544228327 1106 26-27 0.43111415208419146 1106 28-29 0.39821181756692425 1106 30-31 0.4823593043938672 1106 32-33 0.4082284528890412 1106 34-35 0.4453464484283671 1106 36-37 0.3823423987652461 1106 38-39 0.2846364727070565 1106 40-41 0.1785537875880152 1106 42-43 0.6069102702341667 1106 44-45 0.3616907324490626 1106 46-47 0.3961853686135939 1106 48-49 0.2511822180463632 1106 50-51 0.3141197572001353 1106 52-53 0.24155898894262862 1106 54-55 0.28288530114415167 1106 56-57 0.24415134055835352 1106 58-59 0.18795571115128595 1106 60-61 -0.005135155537089986 1106 62-63 0.0879817009166608 1106 64-65 0.10795380762789009 1106 66-67 -0.016388736611968824 1106 68-69 -0.06501180781096139 1106 70-71 -0.09241503518007121 1106 72-73 0.017284091905310106 1106 74-75 -0.13321388219579333 1106 76-77 -0.1084525977694959 1106 78-79 -0.05922734126068008 1106 80-81 -0.18409941062157387 1106 82-83 -0.5828099348516282 1106 84-85 -0.5766689472155271 1106 86-87 -0.2769064898197229 1106 88-89 -0.29290051789807947 1106 90-91 -3.5125181647379407 1106 92-93 -8.735281705113875 1106 94-95 -5.8599817882842515 1106 96-97 -2.7672724515617766 1106 98-99 -1.649075477533227 1106 100-101 -1.088669225242553 1107 1 1.578319835825365 1107 2 0.8532014422583813 1107 3 0.5390737859200918 1107 4 0.6875376545466665 1107 5 0.34688031288448684 1107 6 0.48141547451592714 1107 7 0.40553615500258644 1107 8 0.517282808935974 1107 9 0.4173953053719046 1107 10-11 0.405355308909499 1107 12-13 0.24176939765929006 1107 14-15 0.42887287212592184 1107 16-17 0.4150157839534643 1107 18-19 0.5614895227013221 1107 20-21 0.3707666637922884 1107 22-23 0.3688333671045072 1107 24-25 0.3438174000810861 1107 26-27 0.40655266749035235 1107 28-29 0.2323819856341558 1107 30-31 0.43514291783925074 1107 32-33 0.6212788730571148 1107 34-35 0.5292335632743033 1107 36-37 0.3979674687932544 1107 38-39 0.3881943158443093 1107 40-41 0.2014853001930561 1107 42-43 0.2581503262565761 1107 44-45 -0.09429400144449573 1107 46-47 0.029130746764842286 1107 48-49 0.1358431424161033 1107 50-51 0.3727703174242265 1107 52-53 0.12807033348044428 1107 54-55 -0.0030848669230749692 1107 56-57 0.055879071650789314 1107 58-59 -0.03923882666384415 1107 60-61 -0.28537493144745696 1107 62-63 -0.188035666030121 1107 64-65 -0.25915935763821807 1107 66-67 -0.22391394669600473 1107 68-69 -0.045244020696117104 1107 70-71 0.009495328965584804 1107 72-73 -0.07800624422914382 1107 74-75 -0.12894287379243252 1107 76-77 -0.13057360617285596 1107 78-79 -0.3077540919609625 1107 80-81 -0.44347518092969906 1107 82-83 -0.5894037723866425 1107 84-85 -0.5539940172435358 1107 86-87 -0.17799416488974984 1107 88-89 -0.45937600809415713 1107 90-91 -3.5504026185194526 1107 92-93 -4.9393668591755 1107 94-95 -3.156799435343075 1107 96-97 -1.85746278769623 1107 98-99 -1.2845725363567553 1107 100-101 -0.9399253877075395 1108 1 1.625888345038092 1108 2 0.9113240553237105 1108 3 -0.5680015909643323 1108 4 -0.5027672030747752 1108 5 0.13664781707209528 1108 6 0.40328214118259353 1108 7 0.4213364900109582 1108 8 0.539985656507163 1108 9 0.44117687991126786 1108 10-11 0.5665687092445069 1108 12-13 0.2889265165872672 1108 14-15 0.30927354214267666 1108 16-17 0.2992261692130924 1108 18-19 0.29689253777670643 1108 20-21 0.16919279444555002 1108 22-23 0.23484408737251528 1108 24-25 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2311 78-79 0.6397362602595607 2311 80-81 0.680823338723183 2311 82-83 1.0442603419574112 2311 84-85 1.3460598878100605 2311 86-87 1.7431541761005533 2311 88-89 2.148973149639378 2311 90-91 2.7982814039409867 2311 92-93 3.7427468723201613 2311 94-95 3.34191256057321 2311 96-97 2.5765496676381225 2311 98-99 2.6020402252563137 2311 100-101 2.2528190737523897 2312 1 1.340680580326211 2312 2 0.5281212392126982 2312 3 -0.13465210240478243 2312 4 -0.6446721593281239 2312 5 0.027681666522390458 2312 6 -0.3632153207463418 2312 7 0.008105359740319784 2312 8 0.25620125225238155 2312 9 0.15772153210625817 2312 10-11 0.06270420907870289 2312 12-13 0.09875484605184681 2312 14-15 0.0817960531749975 2312 16-17 -0.14520282856768318 2312 18-19 -0.5648272285677081 2312 20-21 0.33335752673644947 2312 22-23 0.39215857861043446 2312 24-25 0.3270999719931069 2312 26-27 0.3787245795038885 2312 28-29 0.29898097040572935 2312 30-31 0.3442799736768123 2312 32-33 0.485674812143408 2312 34-35 0.24382307258056812 2312 36-37 0.3642327818220181 2312 38-39 0.3771398319187611 2312 40-41 0.2977858078072657 2312 42-43 0.44268907414151926 2312 44-45 0.32429550786176975 2312 46-47 0.43190232036891274 2312 48-49 0.303461416527778 2312 50-51 0.321558811502058 2312 52-53 0.24412718627232266 2312 54-55 0.2708217320616946 2312 56-57 0.2690628279959668 2312 58-59 0.2409195489706768 2312 60-61 0.1768125474019513 2312 62-63 0.39249157593265593 2312 64-65 0.37449072696415087 2312 66-67 0.636258642136486 2312 68-69 0.5664906662751186 2312 70-71 0.5513464287963785 2312 72-73 0.3029602532327864 2312 74-75 0.572701796258329 2312 76-77 1.0126304547408473 2312 78-79 1.0067745036397149 2312 80-81 1.0326825178858705 2312 82-83 1.0408026573933924 2312 84-85 1.4180269641439445 2312 86-87 1.8000775779190477 2312 88-89 2.0890063946131185 2312 90-91 2.9641963662521214 2312 92-93 4.256317066374418 2312 94-95 3.7436797524741827 2312 96-97 2.5840762985981875 2312 98-99 2.4414944687193874 2312 100-101 2.658879350025119 2313 1 -7.486292016781192 2313 2 -2.7313544415928668 2313 3 -1.5646939165760898 2313 4 -0.7470597304083384 2313 5 -0.4137828896965914 2313 6 -0.23426243344807318 2313 7 -0.23375173767868773 2313 8 -0.05039876688335454 2313 9 -0.03513463179908882 2313 10-11 -0.13581419355272573 2313 12-13 -0.4024191572736342 2313 14-15 -0.5742600197063013 2313 16-17 -0.6359668616569962 2313 18-19 -0.6592428257449257 2313 20-21 -0.4372916143534127 2313 22-23 -1.0827168451556375 2313 24-25 -0.2172659757042723 2313 26-27 -0.4629235763541075 2313 28-29 -0.5955446572593743 2313 30-31 -0.10214694461618024 2313 32-33 -0.4865438472689192 2313 34-35 -0.1760899891115031 2313 36-37 -0.25703827072108965 2313 38-39 -0.36667286024670886 2313 40-41 -0.4257868342949749 2313 42-43 -0.6485384833885277 2313 44-45 -0.6827291177630528 2313 46-47 -1.457734255782274 2313 48-49 -0.8043997511142074 2313 50-51 -0.8330989303258178 2313 52-53 -1.2184743430350977 2313 54-55 -0.9503704502139563 2313 56-57 -0.6793889874424366 2313 58-59 -0.6625476397051067 2313 60-61 -0.466436028407891 2313 62-63 -0.5029607127273543 2313 64-65 -0.5071252601683582 2313 66-67 -0.378923625758663 2313 68-69 -0.3064053723687259 2313 70-71 -0.40484734381417553 2313 72-73 -0.2872463868679631 2313 74-75 -0.09787002611031426 2313 76-77 0.026239842629998122 2313 78-79 -0.0716858970212435 2313 80-81 -1.542775505178895E-4 2313 82-83 0.28923572498133154 2313 84-85 0.5368902755056588 2313 86-87 1.0773160066164493 2313 88-89 1.5347445556687909 2313 90-91 2.032635588306835 2313 92-93 2.9836551819180634 2313 94-95 2.747804538166857 2313 96-97 1.8928239163282221 2313 98-99 1.7382602705714163 2313 100-101 1.3376963477314145 2314 1 0.8767259533088527 2314 2 0.10482021180999723 2314 3 -1.130402752140391 2314 4 0.041137932614098816 2314 5 0.041763594080180155 2314 6 -3.0451015918726725 2314 7 -2.406523351427726 2314 8 -2.3410357173066423 2314 9 -2.991912445757741 2314 10-11 -2.538440172494745 2314 12-13 -1.130154550898233 2314 14-15 -1.1073950458441857 2314 16-17 -2.1194862667938423 2314 18-19 -2.982938840176672 2314 20-21 -2.736565140170171 2314 22-23 -3.853438304775924 2314 24-25 -3.063798171695062 2314 26-27 -1.7038820909385706 2314 28-29 -1.518879745335603 2314 30-31 -1.786546341806222 2314 32-33 -1.818041252073236 2314 34-35 -1.3725589984219084 2314 36-37 -1.6894216170600629 2314 38-39 -2.9883021735543664 2314 40-41 -2.290207713362733 2314 42-43 -1.6449873866388032 2314 44-45 -1.9151314015140102 2314 46-47 -1.6686148215354635 2314 48-49 -2.202835064048962 2314 50-51 -2.928690231758953 2314 52-53 -1.9812201774684652 2314 54-55 -2.485242114055506 2314 56-57 -1.4886585717277292 2314 58-59 -0.8742165117524756 2314 60-61 -1.1902544587328237 2314 62-63 -1.029427671591467 2314 64-65 -1.6084404142944564 2314 66-67 -1.0574509644228023 2314 68-69 -1.0544117648740823 2314 70-71 -0.9576238924456035 2314 72-73 -1.8838364840623036 2314 74-75 -1.9567030406328918 2314 76-77 -1.071036171344911 2314 78-79 -1.8514799174736467 2314 80-81 -0.9661889800259793 2314 82-83 -0.2731736715871982 2314 84-85 -1.428718800003356 2314 86-87 -0.46374710197699187 2314 88-89 0.48336573677897476 2314 90-91 1.713488935182319 2314 92-93 2.8618783267820938 2314 94-95 2.661268135042743 2314 96-97 1.7967399234612245 2314 98-99 1.5944935741750506 2314 100-101 1.6824101006483883 2315 1 -2.4579424109518015 2315 2 -1.4716515964156507 2315 3 -2.3230919599362636 2315 4 -3.721537851899008 2315 5 -3.793722265384389 2315 6 -2.8972703450052535 2315 7 -5.301087786065555 2315 8 -2.8227201376570292 2315 9 -2.1083608640571896 2315 10-11 -3.646976919451461 2315 12-13 -2.6327550958950354 2315 14-15 -1.7228678276899103 2315 16-17 -2.341506499656113 2315 18-19 -1.6576883778511586 2315 20-21 -0.9506189715668256 2315 22-23 -0.5382576089544244 2315 24-25 -2.161412802497175 2315 26-27 -3.740403501938747 2315 28-29 -3.1435296165757904 2315 30-31 -3.1388902313320237 2315 32-33 -3.7490084197605675 2315 34-35 -3.478694245197147 2315 36-37 -2.7687831757739616 2315 38-39 -1.7538933452974987 2315 40-41 -1.4994671859947886 2315 42-43 -2.8213796921596312 2315 44-45 -6.134133412125891 2315 46-47 -2.9302173195334937 2315 48-49 -1.8813480178048856 2315 50-51 -3.015568540770989 2315 52-53 -2.977213644420111 2315 54-55 -1.5448484028346776 2315 56-57 -1.1586203758630234 2315 58-59 -2.2357161747301397 2315 60-61 -2.4663613034548213 2315 62-63 -4.346826089602864 2315 64-65 -3.670217184525878 2315 66-67 -3.3637371511158953 2315 68-69 -1.8076756965708896 2315 70-71 -3.0895249288613016 2315 72-73 -2.3554139790357738 2315 74-75 -1.4319704981018262 2315 76-77 -2.8722995730236853 2315 78-79 -1.598422231924129 2315 80-81 -1.3083893614085191 2315 82-83 -0.9534074556278931 2315 84-85 -2.2140102235050456 2315 86-87 -1.7542203159026428 2315 88-89 -1.0244551977810836 2315 90-91 -0.06892195553602676 2315 92-93 0.444742901413818 2315 94-95 0.34348343758510325 2315 96-97 -0.14722190371832866 2315 98-99 -0.5536355197821692 2315 100-101 -0.7707305442452927 2316 1 -7.233748640796055 2316 2 -6.126682720637547 2316 3 -3.4282655805957454 2316 4 -3.6599706410792763 2316 5 -1.986634317583082 2316 6 -2.9298626551974962 2316 7 -3.3087682190547305 2316 8 -2.513585366026323 2316 9 -7.155665931430505 2316 10-11 -6.924101553835602 2316 12-13 -5.849412804452321 2316 14-15 -5.170732256079198 2316 16-17 -4.746463250737335 2316 18-19 -4.040000070058852 2316 20-21 -2.7959993995561305 2316 22-23 -3.2479070552182634 2316 24-25 -3.077170533553904 2316 26-27 -4.205581872479485 2316 28-29 -4.0483985573522645 2316 30-31 -6.753601426052157 2316 32-33 -4.7725650183456025 2316 34-35 -5.440278955579391 2316 36-37 -4.3001277046607385 2316 38-39 -6.310244749012401 2316 40-41 -3.618654518811468 2316 42-43 -4.641837305719292 2316 44-45 -3.03203502708552 2316 46-47 -4.32294843875551 2316 48-49 -5.153209346271677 2316 50-51 -4.402004192379696 2316 52-53 -6.091363603171139 2316 54-55 -5.0038188035746565 2316 56-57 -4.424643100294912 2316 58-59 -2.862161451098231 2316 60-61 -2.8533494412513747 2316 62-63 -4.032170205999954 2316 64-65 -2.692870368196285 2316 66-67 -3.8212125892299404 2316 68-69 -3.0097031458846537 2316 70-71 -2.4181260888323024 2316 72-73 -1.927871855088874 2316 74-75 -1.898707579674788 2316 76-77 -2.630430619747518 2316 78-79 -4.647644438868955 2316 80-81 -5.5177088159221555 2316 82-83 -2.924115687922086 2316 84-85 -1.523723730667367 2316 86-87 -1.8922103036529485 2316 88-89 -0.9437772147306589 2316 90-91 -0.7993030710081399 2316 92-93 -0.8629481563097379 2316 94-95 -1.609133371994659 2316 96-97 -3.2735143714066375 2316 98-99 -4.703515522782094 2316 100-101 -4.047561435973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13546.0 3 4304.0 4 4026.0 5 4414.0 6 4859.0 7 6012.0 8 7096.0 9 8531.0 10 10342.0 11 12700.0 12 15650.0 13 18446.0 14 20973.0 15 24342.0 16 28539.0 17 30562.0 18 26449.0 19 23467.0 20 24721.0 21 27375.0 22 30030.0 23 34421.0 24 38625.0 25 44089.0 26 50617.0 27 57529.0 28 66125.0 29 75922.0 30 86472.0 31 98595.0 32 110929.0 33 124059.0 34 142820.0 35 166611.0 36 181928.0 37 152375.0 38 49199.0 39 1013.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88441160502335 20.096662873709878 13.588354435650215 21.43057108561655 2 15.636261850362926 21.103903039970376 40.51913449829835 22.74070061136835 3 19.257824419012955 26.778020576607354 29.73336744325163 24.230787561128057 4 12.210355380675786 17.057735526124034 37.87341378241056 32.85849531078962 5 13.003139391502552 38.65510681107174 33.507184008002135 14.834569789423577 6 30.108589649466914 36.865912054437274 18.330721776005085 14.69477652009073 7 27.58095887400454 34.190515388885935 20.930682466496517 17.297843270613004 8 26.57474899911995 34.31393823422073 20.238216575866872 18.873096190792452 9 27.833628357382818 16.319141123795013 20.41831304700473 35.42891747181744 10-11 24.84028614235001 27.300438507561818 27.113680975610826 20.745594374477346 12-13 26.243462723172883 25.097389637178225 27.804765386795555 20.854382252853334 14-15 23.054199177277653 25.72481378605164 27.12830227347326 24.09268476319745 16-17 21.436469598074755 29.30448281001372 27.39671871369895 21.862328878212576 18-19 21.207345039456857 27.188445096479473 30.996027049711067 20.608182814352602 20-21 22.630253505534046 26.149050621637986 30.84606889672616 20.37462697610181 22-23 21.992374954015624 26.39338833732002 30.607860025471645 21.00637668319272 24-25 21.279860931174365 26.6406460997254 31.131126931835162 20.94836603726507 26-27 21.294724328800857 26.844291122387837 31.010531598222475 20.85045295058883 28-29 20.95085190308857 26.989430561020722 31.11851783807055 20.941199697820164 30-31 21.150961927067595 26.927103240386074 31.055961929809456 20.86597290273687 32-33 20.947621076598356 26.928805309564662 30.835275943927787 21.288297669909195 34-35 21.45631232988479 26.894817531215658 30.82638433481525 20.8224858040843 36-37 21.414678149479464 26.7320594759253 30.684609247119017 21.168653127476222 38-39 21.106156382635167 26.994890470527405 30.80143235699047 21.097520789846953 40-41 21.29719848159929 26.85516358777274 30.832137544338323 21.015500386289652 42-43 21.170576960190886 26.910133428075163 30.473890073161748 21.445399538572204 44-45 21.160591054685305 27.19948027099372 30.300283301649785 21.33964537267119 46-47 21.27023980843619 27.30697283830662 29.76359119637731 21.659196156879883 48-49 21.33328908491062 27.44223180998996 29.531351607383378 21.69312749771604 50-51 21.24460647124713 27.65167431149884 29.34850341576441 21.75521580148962 52-53 21.426596642204913 27.767855085788014 29.117127870708543 21.68842040129853 54-55 21.03275692209123 27.562034970850313 29.382244910049565 22.02296319700889 56-57 21.17549153615932 27.904721297181094 29.046047625641453 21.87373954101813 58-59 21.322546511178338 27.515664592495746 29.066480500389506 22.095308395936406 60-61 21.24345031283622 27.54715843287213 29.28473235884679 21.924658895444857 62-63 21.229450096674793 27.3426300477426 29.27982624302623 22.148093612556373 64-65 21.054179533467572 27.291231644079755 29.27400269387366 22.380586128579015 66-67 21.07301917468456 27.5524556419746 28.965371861248407 22.409153322092433 68-69 21.171687311746368 27.377930506655147 28.965182055721428 22.485200125877057 70-71 21.09712576340827 27.447607946299478 28.876639144949838 22.57862714534241 72-73 21.256948893003504 27.366270830225435 28.66111230551875 22.715667971252316 74-75 21.036892120801273 27.46633736920175 28.63892483609855 22.85784567389843 76-77 21.17291181603526 27.383575932329187 28.69111736880085 22.752394882834707 78-79 21.202329939025326 27.468854426533092 28.52551655136551 22.803299083076073 80-81 21.064901550191546 27.61052047748335 28.46864753097832 22.855930441346782 82-83 21.323069352366904 27.532093913011995 28.107413014324827 23.037423720296278 84-85 21.184694654635912 27.2760803862727 28.107196322654087 23.432028636437305 86-87 21.050672919366228 27.113624988858952 28.628149488882947 23.207552602891873 88-89 21.32536507545592 27.019464383220143 28.32846218674761 23.326708354576322 90-91 21.10835009316718 27.114411387032767 28.507481265578814 23.269757254221236 92-93 21.533430864560387 26.846240809663296 28.30147606797244 23.318852257803872 94-95 21.22664492601453 26.655000281365766 28.43191428912597 23.68644050349374 96-97 21.578641406882483 26.622331458400833 27.959959496410118 23.839067638306563 98-99 21.54519513693093 26.52357850840049 27.758281251629413 24.17294510303917 100-101 21.806225621339344 26.571220204545387 27.781222457997544 23.84133171611773 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3733.0 1 2421.0 2 1243.5 3 1410.5 4 1287.5 5 1211.0 6 1697.5 7 3113.0 8 4837.5 9 5351.5 10 4435.5 11 3542.5 12 3363.5 13 3371.5 14 3425.0 15 3572.0 16 3686.5 17 3880.5 18 4216.5 19 4582.5 20 5048.5 21 5718.0 22 6578.5 23 7526.0 24 8690.5 25 10368.5 26 12599.0 27 15121.5 28 17517.0 29 20215.0 30 23576.5 31 26928.5 32 30524.0 33 34150.5 34 37659.0 35 41036.5 36 44790.0 37 48267.5 38 50146.0 39 51364.0 40 53049.5 41 55859.5 42 58749.5 43 61135.5 44 63037.0 45 64621.5 46 65967.5 47 67346.0 48 70415.0 49 70948.0 50 67337.0 51 63756.0 52 60514.0 53 57339.5 54 54317.0 55 50337.0 56 45315.5 57 40587.5 58 36224.5 59 31765.0 60 27194.0 61 22501.5 62 18279.0 63 14451.5 64 11012.5 65 8564.5 66 6596.0 67 5065.5 68 3796.5 69 2634.0 70 1889.5 71 1455.0 72 1194.5 73 977.0 74 819.0 75 701.5 76 493.0 77 322.0 78 225.0 79 155.0 80 110.5 81 91.5 82 64.5 83 45.0 84 35.0 85 21.5 86 15.0 87 9.5 88 6.5 89 5.5 90 5.5 91 3.0 92 1.0 93 2.0 94 1.5 95 0.5 96 2.0 97 2.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13131164466193543 2 0.11686736374912254 3 0.12266696138835802 4 0.134539722593208 5 0.12036900760677414 6 0.13639997089258543 7 0.16698464146176123 8 0.15423646929249832 9 0.1643036953832467 10-11 0.15795696589125316 12-13 0.14764353046676365 14-15 0.15297806603115477 16-17 0.14756146068884993 18-19 0.16405748604950557 20-21 0.16950144798444833 22-23 0.20375190196710316 24-25 0.22736064141361362 26-27 0.24021823995342811 28-29 0.23502048735222653 30-31 0.2261843079301838 32-33 0.22629373430073538 34-35 0.21606236865415962 36-37 0.22530889696577092 38-39 0.21152117427626765 40-41 0.21622650820998704 42-43 0.20840252271554668 44-45 0.20164544433398462 46-47 0.21811411310200235 48-49 0.25564735820120554 50-51 0.2568784048699112 52-53 0.25884807953984024 54-55 0.2576443894637725 56-57 0.23091699845654104 58-59 0.23272253357064265 60-61 0.20109831248122656 62-63 0.19456008684076767 64-65 0.1955449241757322 66-67 0.20350569263336205 68-69 0.20304063055851768 70-71 0.20900436775358058 72-73 0.24079272839882412 74-75 0.24278975966139102 76-77 0.257781172426962 78-79 0.2550181565705338 80-81 0.26842288696310634 82-83 0.2680125380735378 84-85 0.24300861240249427 86-87 0.24623669033376683 88-89 0.24962890782086683 90-91 0.4450644056260474 92-93 0.594130478909982 94-95 0.34143763271367006 96-97 0.28223796624524744 98-99 0.31383483074202567 100-101 0.33175339891985234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1827713.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.203159163323484 #Duplication Level Percentage of deduplicated Percentage of total 1 78.28621106871502 45.565048031259565 2 12.199988413380085 14.201557348293267 3 4.184162407795468 7.305944117583432 4 1.8674168628982646 4.347582435821677 5 0.9873047653011365 2.873212819876489 6 0.5767869750089516 2.0142494465866734 7 0.3875031322185552 1.5787734536562066 8 0.26022417928557584 1.2116695460082876 9 0.18337476475504527 0.9605691557617411 >10 0.9445708119890474 10.155149275975289 >50 0.08225274079300451 3.3188026922724982 >100 0.03818922921816067 3.8965120345981754 >500 0.0011512277952843918 0.42341636030693897 >1k 5.756138976421959E-4 0.5540762947811888 >5k 1.9187129921406532E-4 0.8742489718219363 >10k+ 9.593564960703266E-5 0.719188015396726 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12880 0.7047058263523869 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9096 0.49767113326873536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6561 0.358973208594566 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2641 0.1444975223134048 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1879 0.10280607513324029 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1844 0.10089111364858705 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 5.471318527580643E-5 0.0 0.0 2.7356592637903216E-5 0.0 16-17 8.206977791370965E-5 0.0 0.0 8.206977791370965E-5 0.0 18-19 1.3678296318951608E-4 0.0 0.0 1.641395558274193E-4 0.0 20-21 1.641395558274193E-4 0.0 0.0 3.0092251901693535E-4 0.0 22-23 1.641395558274193E-4 0.0 0.0 6.018450380338707E-4 0.0 24-25 1.641395558274193E-4 0.0 0.0 0.0015593257803604831 0.0 26-27 1.641395558274193E-4 0.0 0.0 0.003200721338634676 0.0 28-29 2.4620933374112897E-4 0.0 0.0 0.011134133203626608 0.0 30-31 2.7356592637903216E-4 0.0 0.0 0.0348522990206887 0.0 32-33 3.0092251901693535E-4 0.0 0.0 0.08351967732351852 0.0 34-35 3.282791116548386E-4 0.0 0.0 0.16151332293418058 0.0 36-37 3.282791116548386E-4 0.0 0.0 0.2931532467077709 0.0 38-39 3.82992296930645E-4 0.0 0.0 0.4925828070380853 0.0 40-41 3.82992296930645E-4 0.0 0.0 0.7171257194099949 0.0 42-43 4.3770548220645146E-4 0.0 0.0 0.937866065405236 0.0 44-45 6.018450380338707E-4 0.0 0.0 1.1906409813794616 0.0 46-47 7.112714085854837E-4 0.0 0.0 1.4904692366908807 0.0 48-49 7.6598459386129E-4 0.0 0.0 1.8160126890819293 0.0 50-51 7.6598459386129E-4 0.0 0.0 2.181223200797937 0.0 52-53 7.6598459386129E-4 0.0 0.0 2.5188035539496627 0.0 54-55 7.6598459386129E-4 0.0 0.0 2.8662049238583958 0.0 56-57 7.6598459386129E-4 0.0 0.0 3.267854416968091 0.0 58-59 7.6598459386129E-4 0.0 0.0 3.7051769068776115 0.0 60-61 8.206977791370964E-4 0.0 0.0 4.181099548999214 0.0 62-63 8.206977791370964E-4 0.0 0.0 4.631881482486583 0.0 64-65 8.206977791370964E-4 0.0 0.0 5.074429081589943 0.0 66-67 8.206977791370964E-4 0.0 0.0 5.56569877218141 0.0 68-69 8.206977791370964E-4 0.0 0.0 6.1084809267100475 0.0 70-71 8.754109644129029E-4 0.0 0.0 6.700696444135376 0.0 72-73 9.301241496887093E-4 0.0 0.0 7.273871773084724 0.0 74-75 9.301241496887093E-4 0.0 0.0 7.818514175912739 0.0 76-77 9.301241496887093E-4 0.0 0.0 8.382059984253544 0.0 78-79 9.301241496887093E-4 0.0 0.0 8.994191101119267 0.0 80-81 9.848373349645157E-4 0.0 0.0 9.63099786454438 0.0 82-83 9.848373349645157E-4 0.0 0.0 10.233335321245733 0.0 84-85 9.848373349645157E-4 0.0 0.0 10.796634920252796 2.7356592637903216E-5 86-87 0.0010395505202403222 0.0 0.0 11.351946394209595 5.471318527580643E-5 88-89 0.0010395505202403222 0.0 0.0 11.943860989115906 5.471318527580643E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6075 0.0 42.721123 1 GTATCAA 10925 0.0 41.083866 1 TATCAAC 14240 0.0 32.51932 2 ATCAACG 14285 0.0 32.25346 3 TCAACGC 14425 0.0 31.874638 4 CAACGCA 14745 0.0 31.086346 5 AACGCAG 15240 0.0 30.23233 6 GTACATG 12455 0.0 27.846798 1 TACATGG 12785 0.0 27.016699 2 ACGCAGA 17180 0.0 26.707956 7 CGCAGAG 17285 0.0 26.517538 8 ACATGGG 13175 0.0 25.13866 3 GCAGAGT 17820 0.0 24.576468 9 TACGCGC 50 0.0015945836 23.90056 88-89 GAGTACT 9380 0.0 23.015274 12-13 CATGGGG 3925 0.0 22.848593 4 AGTACTT 9705 0.0 22.097872 12-13 CATGGGA 8615 0.0 21.976318 4 GTACTTT 10265 0.0 20.847544 14-15 AGAGTAC 16805 0.0 20.694511 10-11 >>END_MODULE