FastQCFastQC Report
Fri 27 May 2016
SRR939733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827713
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132550.7252232708308142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103200.5646400720463224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79410.4344774042751789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31520.17245595998934188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.13421144348155317No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.11779748789881124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63800.044.1334721
GTATCAA109300.039.0519641
TATCAAC143050.029.6002852
ATCAACG142800.029.5200273
TCAACGC143900.029.2919654
CAACGCA146350.028.8338135
AACGCAG150750.028.0355956
GTACATG124950.027.6305331
TACATGG124950.027.1708772
ACATGGG124550.025.7816563
ACGCAGA173400.024.2919887
CGCAGAG174250.024.065338
CATGGGA81900.023.2578324
GCAGAGT183500.022.467089
GAGTACT100800.021.31497812-13
ATGGGAG29100.021.2251175
AGTACTT102700.020.46185712-13
CGCGGAA1850.020.37589582-83
ATGGGAT32950.020.1760185
GTACTTT108450.019.83342714-15
Produced by FastQC (version 0.11.5)