FastQCFastQC Report
Fri 27 May 2016
SRR939732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904075
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133610.7017055525648938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94700.4973543584155036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69360.36427136536113336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27780.14589761432716675No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.10829405354305897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20530.10782138308627548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67400.044.1162641
GTATCAA116200.038.0595171
TATCAAC148500.029.65532
ATCAACG150950.029.2363173
TCAACGC151950.029.043914
CAACGCA153850.028.5934815
AACGCAG158650.028.0256756
GTACATG122450.027.0492611
TACATGG124800.026.3144192
ACATGGG130900.024.7287273
ACGCAGA178300.024.7259277
CGCAGAG180050.024.4065678
GCAGAGT184800.022.786319
CATGGGG39650.022.3083574
GAGTACT102200.022.18841412-13
CATGGGA85550.021.88854
AGTACTT104900.021.19124412-13
GCTCGCG1106.3390053E-620.6213694-95
GTACTTT111000.020.49694814-15
AGAGTAC175700.019.77517310-11
Produced by FastQC (version 0.11.5)