FastQCFastQC Report
Fri 27 May 2016
SRR939732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904075
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137280.720980003413731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111040.5831703057915261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84760.44515053241075064No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32240.16932106140777017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25910.13607657261399891No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23020.12089859905728503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68850.046.461831
GTATCAA117050.037.8717841
ATCAACG149300.029.5159443
TATCAAC150150.029.4182192
TCAACGC151550.029.0777344
CAACGCA154650.028.4641915
AACGCAG158600.027.7552766
GTACATG126450.027.3245411
TACATGG127350.026.6378442
ACATGGG128000.025.1628933
ACGCAGA181450.024.1816277
CGCAGAG182750.024.0096118
CATGGGA84950.022.6147824
GCAGAGT190250.022.6143139
GAGTACT103200.022.17779512-13
ATGGGAG30250.021.169435
AGTACTT105100.021.07729712-13
ATGGGCG2751.2823875E-920.6989975
GTACTTT111000.020.64072214-15
GTCGGGA3950.020.4194412
Produced by FastQC (version 0.11.5)