FastQCFastQC Report
Fri 27 May 2016
SRR939731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791400
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263573.3304270912307303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171812.1709628506444276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167302.1139752337629516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150531.902072276977508No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68600.8668182966894111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61460.7765984331564316No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48700.6153651756381097No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47550.6008339651250948No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27510.3476118271417741No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.32878443265099827No Hit
GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA18010.22757139246904218No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.2269396007076068No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10640.1344452868334597No Hit
GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA9670.12218852666161233No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9640.12180945160475108No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.11738690927470306No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.11700783421784179No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.10677280768258782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG751.3767749E-744.272871
GATAGTC1400.040.6587527
TAGTCAA1400.040.656189
GTATCAA127800.039.6406971
GGTATCA72750.039.5782471
TATAACG851.6468715E-533.483682
TATCAAC160850.032.3214152
TACATGG114350.032.1904032
GGGACCG902.4346205E-531.6234767
GTACATG115100.031.4861011
ATACATG2300.030.936011
ATCAACG168100.030.9293793
TCAACGC169950.030.5647814
ACATGGG117150.030.2486953
CAACGCA172650.030.0299385
AACGCAG176250.029.5511286
CATAAGG650.0058802629.192753
TAGATAG1951.6370905E-1129.19095
CATGGGA90950.029.0523224
ATGGGAC23700.029.0215475
Produced by FastQC (version 0.11.5)