##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939731_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 791400 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.703097043214555 31.0 31.0 33.0 25.0 34.0 2 30.51325751832196 31.0 31.0 34.0 26.0 34.0 3 30.801789234268384 31.0 31.0 34.0 27.0 34.0 4 34.63133055344958 37.0 35.0 37.0 32.0 37.0 5 34.726023502653526 37.0 35.0 37.0 32.0 37.0 6 34.89782410917362 37.0 35.0 37.0 32.0 37.0 7 34.83716704574172 37.0 35.0 37.0 32.0 37.0 8 34.80063937326257 37.0 35.0 37.0 32.0 37.0 9 36.09058251200404 39.0 35.0 39.0 32.0 39.0 10-11 36.029932398281524 38.0 35.0 39.0 32.0 39.0 12-13 35.9044004296184 38.0 35.0 39.0 31.0 39.0 14-15 36.502837376800606 39.0 36.0 40.0 30.5 41.0 16-17 36.51839714430124 39.0 36.0 40.0 30.5 41.0 18-19 36.64218852666161 39.0 36.0 40.0 31.0 41.0 20-21 36.88265415718979 39.0 36.0 41.0 31.0 41.0 22-23 36.728685873136214 39.0 35.5 41.0 31.0 41.0 24-25 36.477971316654035 39.0 35.0 41.0 30.5 41.0 26-27 36.222890447308565 39.0 35.0 40.5 30.0 41.0 28-29 36.01067917614354 38.0 35.0 40.0 30.0 41.0 30-31 35.47589714430124 38.0 35.0 40.0 29.5 41.0 32-33 34.82583080616629 37.0 34.5 40.0 27.5 41.0 34-35 34.46157695223654 36.0 34.0 40.0 25.5 41.0 36-37 34.08494629770028 35.5 33.5 40.0 24.0 41.0 38-39 33.7057815264089 35.0 33.0 40.0 22.5 41.0 40-41 33.27188463482436 35.0 33.0 39.5 21.0 41.0 42-43 32.63286391205459 35.0 33.0 39.0 17.0 41.0 44-45 31.964510361384885 35.0 32.0 39.0 11.5 41.0 46-47 31.20381981298964 35.0 31.0 39.0 2.0 40.5 48-49 30.5792071013394 35.0 30.0 39.0 2.0 40.0 50-51 29.823440737932778 34.5 28.0 38.5 2.0 40.0 52-53 29.92988501389942 35.0 28.5 38.0 2.0 40.0 54-55 29.979680313368714 35.0 29.0 38.0 2.0 40.0 56-57 29.655025903462217 35.0 28.0 38.0 2.0 40.0 58-59 29.264569749810462 35.0 27.0 38.0 2.0 40.0 60-61 28.846451857467777 35.0 26.5 38.0 2.0 40.0 62-63 28.358429365681072 34.5 26.0 37.0 2.0 40.0 64-65 27.77606835986859 34.0 25.0 36.5 2.0 39.5 66-67 27.153189284811727 33.0 22.5 36.0 2.0 39.0 68-69 26.611773439474348 33.0 21.0 35.5 2.0 39.0 70-71 26.03970495324741 33.0 19.5 35.0 2.0 38.0 72-73 25.376824614607024 32.5 16.5 35.0 2.0 37.5 74-75 25.123588577204952 32.0 12.5 35.0 2.0 37.0 76-77 24.629113596158703 32.0 4.5 35.0 2.0 36.5 78-79 24.01888678291635 31.5 2.0 35.0 2.0 36.0 80-81 23.325737932777358 31.0 2.0 35.0 2.0 35.5 82-83 22.616779125600203 30.5 2.0 34.0 2.0 35.0 84-85 21.91470495324741 29.5 2.0 34.0 2.0 35.0 86-87 21.130026535253982 29.0 2.0 34.0 2.0 35.0 88-89 20.24606141015921 28.0 2.0 34.0 2.0 35.0 90-91 18.940966641394997 25.5 2.0 33.0 2.0 35.0 92-93 17.503740839019457 20.5 2.0 32.5 2.0 35.0 94-95 17.03341546626232 19.5 2.0 32.0 2.0 34.0 96-97 16.81603550669699 19.0 2.0 32.0 2.0 34.0 98-99 16.152311726055093 5.0 2.0 32.0 2.0 34.0 100-101 14.581120798584786 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1663816944113101 1101 2 0.4830815117465441 1101 3 0.4027693278030462 1101 4 0.4439749763585681 1101 5 0.45405031496161286 1101 6 0.3452163365545502 1101 7 0.2385455220963948 1101 8 0.5238949492403293 1101 9 0.43598383628359727 1101 10-11 0.4529058242001227 1101 12-13 0.525815017328739 1101 14-15 0.7575003824748805 1101 16-17 0.5929483800709363 1101 18-19 0.5784925424788483 1101 20-21 0.39877279578072233 1101 22-23 0.38668399081272753 1101 24-25 0.8275058936563937 1101 26-27 0.6756364761595393 1101 28-29 0.7196235228602532 1101 30-31 0.8275828998181183 1101 32-33 0.8951220255769456 1101 34-35 0.6753412675842156 1101 36-37 0.5282674427684597 1101 38-39 0.7122430854854471 1101 40-41 0.6682512327680215 1101 42-43 0.43623188387166323 1101 44-45 0.5008605069814962 1101 46-47 0.7417178307125205 1101 48-49 0.5914771277824116 1101 50-51 0.4683535609932683 1101 52-53 0.5194397772968351 1101 54-55 0.3812766734702855 1101 56-57 0.28432998743700466 1101 58-59 0.06899970256129251 1101 60-61 0.20684911279910878 1101 62-63 0.17321038557486546 1101 64-65 0.017277392322309737 1101 66-67 0.2833021567792109 1101 68-69 -0.11348115240444301 1101 70-71 -0.235544491116066 1101 72-73 -0.05968453401541751 1101 74-75 -0.42403369282771664 1101 76-77 -0.4024418288137461 1101 78-79 -0.5318613392728828 1101 80-81 -0.7502414169345286 1101 82-83 -1.0587887762155184 1101 84-85 -0.9185056581957447 1101 86-87 -1.4060502013469396 1101 88-89 -5.300391240049638 1101 90-91 -8.23188802136065 1101 92-93 -10.152132681295043 1101 94-95 -8.213707314938958 1101 96-97 -4.876965007137684 1101 98-99 -3.6255213310798506 1101 100-101 -2.77923138159775 1102 1 1.8057295674120688 1102 2 1.0843634289714466 1102 3 0.8293928041080463 1102 4 0.8789502701961567 1102 5 0.7670699397305185 1102 6 0.7849326328327209 1102 7 0.6679388473167052 1102 8 0.9692532831483973 1102 9 1.088004046169175 1102 10-11 1.0880488663174006 1102 12-13 1.0999535275727226 1102 14-15 1.2717000296250447 1102 16-17 1.0249680560097332 1102 18-19 0.9834822751272512 1102 20-21 1.146539674617074 1102 22-23 1.3192164781370508 1102 24-25 1.3861187889160576 1102 26-27 1.3521837538096335 1102 28-29 1.0039168109267607 1102 30-31 1.174386894629933 1102 32-33 1.3077527591085811 1102 34-35 1.2495620946232862 1102 36-37 1.031725860550658 1102 38-39 0.8800925925858181 1102 40-41 0.8030438723116688 1102 42-43 0.3749003095963346 1102 44-45 0.576633116846601 1102 46-47 0.7649878987684886 1102 48-49 0.3301357644828009 1102 50-51 0.2564912658675347 1102 52-53 0.536065545123428 1102 54-55 0.5440236516652313 1102 56-57 0.497007663789617 1102 58-59 0.36907334272120806 1102 60-61 0.4086420615869386 1102 62-63 0.1323879295531647 1102 64-65 0.11655255957001387 1102 66-67 -0.029145699980670514 1102 68-69 -0.2428130737451859 1102 70-71 -0.44352232342562914 1102 72-73 -0.27549692291555417 1102 74-75 -0.5943938621778351 1102 76-77 -0.2340995333320528 1102 78-79 -0.42486507912510874 1102 80-81 -0.25332718436066415 1102 82-83 -0.6587892211711655 1102 84-85 -0.7526106187837982 1102 86-87 -0.6022211355090761 1102 88-89 -1.2012173002120932 1102 90-91 -5.938825769840571 1102 92-93 -9.846267943362797 1102 94-95 -7.9432443608339085 1102 96-97 -4.449429371752142 1102 98-99 -3.119882630617319 1102 100-101 -2.318960359111916 1103 1 1.9942249056414951 1103 2 1.29208656957557 1103 3 0.9743079374699057 1103 4 0.7796878865948713 1103 5 0.812275869770481 1103 6 0.8008493118706212 1103 7 0.6412009805825107 1103 8 0.7895036768446033 1103 9 1.0582461438640536 1103 10-11 0.9979419206722113 1103 12-13 0.8838499701891394 1103 14-15 1.0169145467541085 1103 16-17 1.0054178739343413 1103 18-19 0.8230623519161995 1103 20-21 0.8675160412013625 1103 22-23 0.9304782271670007 1103 24-25 0.8191351276723609 1103 26-27 0.8117007227709507 1103 28-29 0.7976003519512176 1103 30-31 0.9612597096523956 1103 32-33 1.0895468203359826 1103 34-35 1.0953503457388791 1103 36-37 1.3537271471585868 1103 38-39 1.3228698318512286 1103 40-41 1.1995934634789052 1103 42-43 0.9435926560257073 1103 44-45 1.1429974888684171 1103 46-47 1.1153418614917854 1103 48-49 1.1288645842092357 1103 50-51 1.1668340508206256 1103 52-53 1.1917596584555383 1103 54-55 1.126589241016081 1103 56-57 1.0706681506630176 1103 58-59 1.1788984280826966 1103 60-61 1.2254583473652652 1103 62-63 1.226805885010915 1103 64-65 1.131412590017792 1103 66-67 0.8814081236026787 1103 68-69 0.749492748883398 1103 70-71 0.5633655408343223 1103 72-73 0.5810288281636531 1103 74-75 0.5005822540709772 1103 76-77 0.36882109576682964 1103 78-79 0.07361325957878861 1103 80-81 -0.11447977547840793 1103 82-83 -0.3984876251369478 1103 84-85 -0.12129728900173475 1103 86-87 0.14081822828950408 1103 88-89 -0.43628158694518504 1103 90-91 -5.706363080014963 1103 92-93 -8.870785538162806 1103 94-95 -7.832288253958124 1103 96-97 -4.31450946455092 1103 98-99 -3.0427087144098426 1103 100-101 -2.307154567047551 1104 1 1.7470593727616794 1104 2 1.0551251183284016 1104 3 0.8836049896241001 1104 4 0.8091663446447583 1104 5 0.6660173663691253 1104 6 0.5888311712810506 1104 7 0.4904073297888587 1104 8 0.5956261258241895 1104 9 0.9788810644989709 1104 10-11 0.8324090408536193 1104 12-13 0.6236458885564815 1104 14-15 0.7232637531033177 1104 16-17 0.8772999601021425 1104 18-19 0.9472120117801452 1104 20-21 0.8941600321310759 1104 22-23 0.6810451205910368 1104 24-25 0.8327405698492285 1104 26-27 0.7929932397777506 1104 28-29 0.6167613496836495 1104 30-31 0.5287200271127119 1104 32-33 0.8168710833745294 1104 34-35 0.7143979647864995 1104 36-37 0.784566149426162 1104 38-39 0.6380625756154004 1104 40-41 0.55957078774194 1104 42-43 0.7389441299486137 1104 44-45 0.5534283278706766 1104 46-47 0.4435731540087957 1104 48-49 0.293263677179759 1104 50-51 0.26320593290679284 1104 52-53 0.124299340995222 1104 54-55 0.23599967185507964 1104 56-57 0.29118969261313055 1104 58-59 0.39205035552033607 1104 60-61 0.1551635627847645 1104 62-63 0.16444760836692396 1104 64-65 0.14104977822640663 1104 66-67 0.31904984695868777 1104 68-69 0.005161683123759531 1104 70-71 0.14669887416765803 1104 72-73 0.17173177600945877 1104 74-75 0.0907183084927432 1104 76-77 0.30589592569880253 1104 78-79 0.18869262465815595 1104 80-81 0.02894426080277057 1104 82-83 0.01024253359321392 1104 84-85 -0.072544454534615 1104 86-87 0.10907219654347244 1104 88-89 -0.055896099416841594 1104 90-91 -2.566340404278 1104 92-93 -7.091307080112919 1104 94-95 -4.543172607699621 1104 96-97 -2.182990190174504 1104 98-99 -1.5126633629359194 1104 100-101 -1.0299436826938102 1105 1 1.8681478081358271 1105 2 1.0482090185551591 1105 3 0.9120063501683191 1105 4 0.811433918340903 1105 5 0.6628994525369265 1105 6 0.5489218742288955 1105 7 0.4496476926006494 1105 8 0.49840390360196807 1105 9 0.7584729012336666 1105 10-11 0.5553115351846927 1105 12-13 0.6490427139533139 1105 14-15 0.760622029747303 1105 16-17 0.759499506587396 1105 18-19 0.5220986330953394 1105 20-21 0.5662688756684844 1105 22-23 0.4666460276205129 1105 24-25 0.564628324951272 1105 26-27 0.6375794075782082 1105 28-29 0.7066706467358017 1105 30-31 0.7766792107861775 1105 32-33 0.9373075913110398 1105 34-35 1.0691598695484004 1105 36-37 0.883630775276508 1105 38-39 0.7944684713070203 1105 40-41 0.6524562752702892 1105 42-43 0.7170903884520143 1105 44-45 0.850026967326464 1105 46-47 1.0102114760042582 1105 48-49 0.8817273960006204 1105 50-51 0.9930812163535059 1105 52-53 0.8244977536936311 1105 54-55 0.6422921888618838 1105 56-57 0.7626466087129025 1105 58-59 0.43298006073575834 1105 60-61 0.47372932242195276 1105 62-63 0.533155091360122 1105 64-65 0.6071212067978387 1105 66-67 0.41236050455506046 1105 68-69 0.3594700731464364 1105 70-71 0.45137574491595345 1105 72-73 0.3368678304312276 1105 74-75 0.20917068944512707 1105 76-77 0.37290272841989136 1105 78-79 0.1466574772658653 1105 80-81 0.027073512503449138 1105 82-83 -0.19072118522764825 1105 84-85 -0.07736304863892585 1105 86-87 -0.07003778078079037 1105 88-89 -0.4096942853578831 1105 90-91 -3.040461719470745 1105 92-93 -7.465825220702488 1105 94-95 -5.265168072552228 1105 96-97 -2.6033133194262046 1105 98-99 -1.6834966962692537 1105 100-101 -1.6382486713559423 1106 1 1.6204087124975715 1106 2 1.0099737244375149 1106 3 0.6827246374832434 1106 4 0.5523302768843266 1106 5 0.7355785241655752 1106 6 0.7385577285706333 1106 7 0.6427349274946081 1106 8 -0.12459529607789932 1106 9 -0.2007908042485269 1106 10-11 -0.404452370377534 1106 12-13 -0.2029180703604112 1106 14-15 0.5486272318281351 1106 16-17 0.8760661332380622 1106 18-19 0.7266904698300394 1106 20-21 0.7832156543799655 1106 22-23 0.7314319419862585 1106 24-25 0.5092111580845184 1106 26-27 0.5835427929323487 1106 28-29 0.49164463633163535 1106 30-31 0.6952866537633753 1106 32-33 0.785791985068542 1106 34-35 0.6066548675476042 1106 36-37 0.5994921198143217 1106 38-39 0.6082839975174963 1106 40-41 0.42064855218104924 1106 42-43 0.902309476087062 1106 44-45 0.5855411730087354 1106 46-47 0.6645282026949353 1106 48-49 0.7645855392579222 1106 50-51 0.6778051059093251 1106 52-53 0.7962014351662212 1106 54-55 0.6478644177534427 1106 56-57 0.5150125550914524 1106 58-59 0.6202749787029447 1106 60-61 0.5311472896088283 1106 62-63 0.5567045111280287 1106 64-65 0.311417925485312 1106 66-67 0.6704237298451758 1106 68-69 0.6742960035185845 1106 70-71 0.5334535760484087 1106 72-73 0.7483624282703616 1106 74-75 0.8219107854835421 1106 76-77 0.5595974062910365 1106 78-79 0.2123687617796719 1106 80-81 0.03664734203526265 1106 82-83 -0.1953730459719445 1106 84-85 -0.2635875384348445 1106 86-87 0.21778234727043255 1106 88-89 -0.10798360108417526 1106 90-91 -2.8712290263935145 1106 92-93 -7.533417257517215 1106 94-95 -4.719609849262913 1106 96-97 -2.0986564566811055 1106 98-99 -1.2379534789823392 1106 100-101 -0.9445762023683457 1107 1 1.7483675883556096 1107 2 0.9266583470044871 1107 3 0.6165545797165457 1107 4 0.6581982651052556 1107 5 0.5635404781779485 1107 6 0.5866508119578313 1107 7 0.5907038586568163 1107 8 0.5400705702686395 1107 9 0.4835034262641926 1107 10-11 0.5694140992872505 1107 12-13 0.4994090143196104 1107 14-15 0.6668796610049341 1107 16-17 0.6835532306411238 1107 18-19 0.7867811727778786 1107 20-21 0.617734077133683 1107 22-23 0.5975373585118433 1107 24-25 0.5420550648780065 1107 26-27 0.47514045763925594 1107 28-29 0.5713226613878177 1107 30-31 0.45934099344795953 1107 32-33 0.6166268831303299 1107 34-35 0.4198314202199498 1107 36-37 0.18758376672949595 1107 38-39 0.01696541880396296 1107 40-41 7.010860150984399E-4 1107 42-43 0.10703239089401251 1107 44-45 -0.11755545025595637 1107 46-47 -0.41880256550978245 1107 48-49 -0.36886784209461965 1107 50-51 -0.25462940635711817 1107 52-53 -0.20384473714885587 1107 54-55 -0.28759181602211825 1107 56-57 -0.1596214010551087 1107 58-59 -0.11152543376973867 1107 60-61 -0.5372749510823205 1107 62-63 -0.6495677413627128 1107 64-65 -0.6938098066331762 1107 66-67 -0.5340375418429844 1107 68-69 -0.6035946399182777 1107 70-71 -0.5039203478801362 1107 72-73 -0.4313556127922169 1107 74-75 -0.5329080651336291 1107 76-77 -0.14657224105507893 1107 78-79 -0.3640720709636831 1107 80-81 -0.48095457052463075 1107 82-83 -0.6585172780237407 1107 84-85 -0.331178677454556 1107 86-87 -0.13443033267334314 1107 88-89 -0.26625716692076296 1107 90-91 -3.200519717028742 1107 92-93 -4.064008981386248 1107 94-95 -2.6078420652262206 1107 96-97 -1.163584992197876 1107 98-99 -0.7129075631801189 1107 100-101 -0.6571894527967235 1108 1 1.6809786514014533 1108 2 1.0205692670023652 1108 3 -0.4062900518237349 1108 4 -0.14858510689550997 1108 5 0.33035650586354137 1108 6 0.4024331494793003 1108 7 0.4062939351164587 1108 8 0.563964028944703 1108 9 0.6194319969753721 1108 10-11 0.5602329161930228 1108 12-13 0.6478704236505877 1108 14-15 0.7512227236088691 1108 16-17 0.6498637839081951 1108 18-19 0.45542497185645914 1108 20-21 0.5321269697874484 1108 22-23 0.24048632754777088 1108 24-25 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56-57 1.3388698823130767 2311 58-59 1.5955631009858564 2311 60-61 1.125164732459357 2311 62-63 1.4676691314427437 2311 64-65 1.476434694209214 2311 66-67 1.5315086092834704 2311 68-69 1.3846333623777198 2311 70-71 1.3748691575213243 2311 72-73 1.2591463432431524 2311 74-75 1.6019252531697923 2311 76-77 1.7550469811972 2311 78-79 1.7254403444572937 2311 80-81 1.967757652513498 2311 82-83 1.9418989788989656 2311 84-85 2.2777910294049555 2311 86-87 2.6370792571246433 2311 88-89 3.1637063175562012 2311 90-91 3.5865486919622924 2311 92-93 4.339476096776437 2311 94-95 4.0569136558014804 2311 96-97 3.2067806067726465 2311 98-99 2.975138791923678 2311 100-101 2.9607667085357576 2312 1 1.457711906395577 2312 2 0.7444235560740466 2312 3 0.12955013619036393 2312 4 -0.3213230622421577 2312 5 0.11179011338410305 2312 6 0.10983620470844357 2312 7 0.2852598575606393 2312 8 0.4311790700727869 2312 9 0.32683505220451536 2312 10-11 0.4491870877558952 2312 12-13 0.43517947182920125 2312 14-15 0.277662206570092 2312 16-17 0.17395309060619724 2312 18-19 -0.2414022901881978 2312 20-21 0.3635018229014335 2312 22-23 0.5254451994631637 2312 24-25 0.3607148617986766 2312 26-27 0.6200601025589023 2312 28-29 0.37391646398162237 2312 30-31 0.3610841125823896 2312 32-33 0.5497530336477894 2312 34-35 0.4171426349224703 2312 36-37 0.3892649031932436 2312 38-39 0.4209597141026009 2312 40-41 0.45437908317135367 2312 42-43 0.6770261782790996 2312 44-45 0.5680869527777475 2312 46-47 0.6941960040105286 2312 48-49 0.8536714674012131 2312 50-51 0.32477082521702627 2312 52-53 0.34208700443506146 2312 54-55 0.5285506522942605 2312 56-57 0.4905416621514327 2312 58-59 0.26449200746509405 2312 60-61 0.2890523317667011 2312 62-63 0.3079942161992477 2312 64-65 0.32955349933882516 2312 66-67 0.3950475394160087 2312 68-69 0.2083453459347524 2312 70-71 0.3017905230481226 2312 72-73 0.0744832408727234 2312 74-75 0.3721659051577326 2312 76-77 0.608477122689937 2312 78-79 0.5797425032856758 2312 80-81 0.812750027527791 2312 82-83 0.7468522577805778 2312 84-85 0.7162201308138201 2312 86-87 1.156200957957946 2312 88-89 1.7226584089513324 2312 90-91 2.4429730351596604 2312 92-93 3.346806277255098 2312 94-95 3.0515265724032865 2312 96-97 1.980760739104376 2312 98-99 1.7994624274507398 2312 100-101 1.5611602872921466 2313 1 -7.687572589708257 2313 2 -2.7943564090161033 2313 3 -1.1581956279391434 2313 4 -0.5359295311228962 2313 5 -0.5076636968768682 2313 6 0.0025919303493608936 2313 7 -0.007146495319560131 2313 8 -0.025468130625959873 2313 9 0.004202745096534954 2313 10-11 0.06259886085008048 2313 12-13 -0.004130037060747327 2313 14-15 -0.2710401917340519 2313 16-17 -0.1355996766098002 2313 18-19 -0.3354984664212495 2313 20-21 -0.09978935625905905 2313 22-23 -0.6906524110081449 2313 24-25 -0.3377283178225241 2313 26-27 -0.5310799523151104 2313 28-29 -0.8462576190862805 2313 30-31 -0.419503486330008 2313 32-33 -0.5502549191073669 2313 34-35 -0.4933449923730251 2313 36-37 -0.1444259841834068 2313 38-39 -0.6609310397226054 2313 40-41 -0.7443383293860464 2313 42-43 -0.6324491181324206 2313 44-45 -0.449042321999535 2313 46-47 -1.6271912855671147 2313 48-49 -1.1460529284979089 2313 50-51 -1.1122488386556633 2313 52-53 -1.1850531436781502 2313 54-55 -0.9881309372146845 2313 56-57 -0.5586159628267993 2313 58-59 -0.77785230021329 2313 60-61 -0.48619663746024955 2313 62-63 -0.3319029738729746 2313 64-65 -0.48798561656161255 2313 66-67 -0.6834776906997035 2313 68-69 -0.5213850693773203 2313 70-71 -0.3224705905844054 2313 72-73 -0.18916567515303484 2313 74-75 -0.2515748514783631 2313 76-77 -0.009291526178099474 2313 78-79 -0.30609089746325324 2313 80-81 0.189838139941358 2313 82-83 0.29250518583419804 2313 84-85 0.04417804154277505 2313 86-87 0.6772833005105348 2313 88-89 0.9585295852872235 2313 90-91 1.547302213406958 2313 92-93 2.540204256432588 2313 94-95 2.3149869532854126 2313 96-97 1.1504046613483716 2313 98-99 1.1390841505385456 2313 100-101 1.3436490060347346 2314 1 0.9442813237430272 2314 2 0.2878877608654804 2314 3 -0.8907904144705512 2314 4 -0.2988417708821487 2314 5 0.03305780295843164 2314 6 -2.2732130084323003 2314 7 -1.8325863014754518 2314 8 -1.674841710433114 2314 9 -2.885796864088732 2314 10-11 -2.0872778929310556 2314 12-13 -0.8148556683661212 2314 14-15 -0.808647735575093 2314 16-17 -1.8419359001111602 2314 18-19 -2.299893848107665 2314 20-21 -2.363350627635228 2314 22-23 -2.88993861580623 2314 24-25 -1.9459065349256903 2314 26-27 -1.0694523764272574 2314 28-29 -1.1847283051894664 2314 30-31 -1.2478764966642615 2314 32-33 -1.255250508483904 2314 34-35 -1.211183033162925 2314 36-37 -1.2827653286195968 2314 38-39 -2.537531984377651 2314 40-41 -1.6529749163451157 2314 42-43 -0.797494368895908 2314 44-45 -1.0282653543869102 2314 46-47 -0.6998563070898385 2314 48-49 -1.523829108897992 2314 50-51 -2.0890274644393863 2314 52-53 -1.050379781052321 2314 54-55 -1.398195393730962 2314 56-57 -0.5700800944085387 2314 58-59 -0.25198804133761143 2314 60-61 -0.5127169067874355 2314 62-63 -0.15883625063122153 2314 64-65 -0.5615020559043735 2314 66-67 -0.010167406130744183 2314 68-69 -0.23340415788569047 2314 70-71 -0.09476517556707265 2314 72-73 -1.2893112536425946 2314 74-75 -1.030807095927397 2314 76-77 -0.04454999429599127 2314 78-79 -0.9230708207519065 2314 80-81 0.1794829451760407 2314 82-83 0.5604749870211556 2314 84-85 -0.8090787014072056 2314 86-87 0.4930204329353174 2314 88-89 1.6334191801240046 2314 90-91 2.428588747606039 2314 92-93 3.5960823892314373 2314 94-95 3.3515099862310933 2314 96-97 2.403353916846296 2314 98-99 2.071723741188025 2314 100-101 2.1248778950600986 2315 1 -1.895451869023173 2315 2 -1.2904108361663909 2315 3 -2.053795785549074 2315 4 -3.6852755868431757 2315 5 -3.5033248598738282 2315 6 -2.5372083796979723 2315 7 -5.061165928806194 2315 8 -2.612037208957787 2315 9 -1.82056726763701 2315 10-11 -3.5002450551176665 2315 12-13 -2.36490463812963 2315 14-15 -1.4989592082237593 2315 16-17 -2.2073844569067305 2315 18-19 -1.6306253378489686 2315 20-21 -1.1129420264237737 2315 22-23 -0.5967984375188706 2315 24-25 -1.9066628838399353 2315 26-27 -3.1858351016413877 2315 28-29 -2.305811503881948 2315 30-31 -2.60698627626914 2315 32-33 -3.016876148677781 2315 34-35 -2.7686063190652455 2315 36-37 -2.032869038469073 2315 38-39 -1.119424556569065 2315 40-41 -0.6268314567716828 2315 42-43 -2.0940499542561284 2315 44-45 -5.107901262635664 2315 46-47 -1.9707261598062544 2315 48-49 -1.4355158833189279 2315 50-51 -2.3168694262242546 2315 52-53 -1.7913843162138612 2315 54-55 -0.40802599577833476 2315 56-57 -0.09121429027511851 2315 58-59 -1.5268417558657212 2315 60-61 -1.6158536228813318 2315 62-63 -3.005582724496339 2315 64-65 -2.7273769927427374 2315 66-67 -2.1327937761924396 2315 68-69 -0.9398873490923414 2315 70-71 -2.595360922371558 2315 72-73 -1.64421337649744 2315 74-75 -0.5233470047251103 2315 76-77 -2.1653322004930047 2315 78-79 -0.9864515746991209 2315 80-81 -0.49729322245650565 2315 82-83 -0.19349171977784252 2315 84-85 -1.1564513332356832 2315 86-87 -0.8834938868216433 2315 88-89 0.13858751758493426 2315 90-91 0.9843791882091466 2315 92-93 1.4805886055842166 2315 94-95 1.3370576678091055 2315 96-97 0.9437492931025098 2315 98-99 0.7827982376104234 2315 100-101 0.11292085121188578 2316 1 -6.877967480084724 2316 2 -5.4196481243019825 2316 3 -2.9600487876762926 2316 4 -3.300157560355835 2316 5 -1.7344063118741033 2316 6 -2.4512034390543107 2316 7 -2.51081596654722 2316 8 -1.9432627630646948 2316 9 -6.716777725332751 2316 10-11 -6.380340093887995 2316 12-13 -5.449484854968993 2316 14-15 -4.223181228398062 2316 16-17 -4.063526809202081 2316 18-19 -3.2906833217918816 2316 20-21 -1.8669391443816394 2316 22-23 -2.538084548820585 2316 24-25 -2.4414806976051224 2316 26-27 -3.4914619458052485 2316 28-29 -3.9106852429885137 2316 30-31 -7.027362142597276 2316 32-33 -4.433181130493473 2316 34-35 -4.9371997795744065 2316 36-37 -4.044846668332518 2316 38-39 -6.213488922176694 2316 40-41 -3.751240466584349 2316 42-43 -4.175484799517918 2316 44-45 -2.8835883208940913 2316 46-47 -3.7750955415395993 2316 48-49 -5.558936764400382 2316 50-51 -5.113967655826947 2316 52-53 -6.299781694927919 2316 54-55 -4.882645154496512 2316 56-57 -4.145498754695623 2316 58-59 -3.1067129217203835 2316 60-61 -3.107035088605617 2316 62-63 -3.809733379817825 2316 64-65 -2.8611432042297302 2316 66-67 -3.7082535305326587 2316 68-69 -2.8995524832445447 2316 70-71 -2.55385608465798 2316 72-73 -2.1002562297191503 2316 74-75 -1.9861300624345724 2316 76-77 -2.576846645013692 2316 78-79 -3.7656545528093197 2316 80-81 -4.397550547646929 2316 82-83 -2.4565106589118564 2316 84-85 -1.301423199014863 2316 86-87 -1.8597307632369287 2316 88-89 -0.8434348868616439 2316 90-91 -0.3338514437815192 2316 92-93 -0.5426735916298071 2316 94-95 -0.9600928845823038 2316 96-97 -2.051370963536481 2316 98-99 -4.044962861682787 2316 100-101 -3.738937894413585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6998.0 3 1374.0 4 1105.0 5 1137.0 6 1221.0 7 1567.0 8 1893.0 9 2479.0 10 3244.0 11 4715.0 12 7165.0 13 9458.0 14 11169.0 15 14438.0 16 17880.0 17 20360.0 18 15382.0 19 11384.0 20 11590.0 21 12395.0 22 13670.0 23 14946.0 24 16245.0 25 18098.0 26 20283.0 27 23288.0 28 26701.0 29 30596.0 30 35882.0 31 41826.0 32 48909.0 33 59014.0 34 75556.0 35 79889.0 36 64212.0 37 50445.0 38 14637.0 39 249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.092606743961255 20.84866320173685 13.497411424146883 16.56131863015501 2 15.5715847105138 24.745352332183014 39.329845263275075 20.353217694028114 3 19.64929769719572 33.85260180322871 28.594344384550475 17.903756115025093 4 10.879208471981478 19.171147690322254 36.15977327074661 33.78987056694966 5 10.855789581549042 43.064760779441926 29.57836103650645 16.501088602502584 6 25.23220225540793 37.823061944640905 19.01926573351367 17.92547006643749 7 24.503355679216558 40.436978661094756 19.542164618751052 15.517501040937642 8 27.82315774554851 31.46875446857351 20.178907447221796 20.529180338656182 9 30.752106833112492 17.010707863021363 20.282041644361254 31.95514365950489 10-11 24.048789160945134 30.797446016376522 23.969380543180254 21.184384279498087 12-13 26.7765799748067 30.012735839208286 26.772594093683388 16.438090092301618 14-15 23.39402030350867 28.719879279854982 29.605539966403356 18.280560450232993 16-17 17.470378037484878 33.47269110657833 28.859206470519755 20.197724385417036 18-19 16.657639062023442 28.97304247572355 37.35890676551472 17.01041169673829 20-21 18.85802660537803 27.898944618745265 37.44686075016991 15.796168025706805 22-23 17.911240442322875 29.28537472044645 37.128699396297264 15.674685440933414 24-25 16.782445512609467 30.05075190826162 37.48784385430859 15.678958724820315 26-27 16.31361821629956 30.53161479061197 37.57605691327185 15.578710079816622 28-29 15.81098545121373 31.273020381724386 37.66253173068947 15.25346243637241 30-31 15.630579410282449 31.771267287466415 37.292683359207025 15.305469943044114 32-33 15.509660099972459 32.27552890748907 36.7067337370642 15.508077255474268 34-35 15.878657273523796 32.26035583872291 36.42548202320599 15.435504864547301 36-37 15.683516093904323 32.59243548509636 36.12847266188872 15.595575759110595 38-39 15.53700142184115 32.81604312875955 36.228082059099464 15.418873390299828 40-41 15.12434213983323 33.01516315058754 36.333317084010474 15.527177625568752 42-43 15.070232712130391 33.304719888939324 35.56227000836247 16.062777390567817 44-45 15.109821767111642 33.32088439770678 35.16144898014733 16.407844855034252 46-47 15.072988957642474 33.521107857028 34.606224288628304 16.79967889670122 48-49 15.379339389928404 33.70074002767583 33.88351451397883 17.036406068416937 50-51 15.301123707938535 34.08059309587458 33.493247809171685 17.125035387015195 52-53 15.36925945442017 34.12616280248065 32.96703366300515 17.537544080094037 54-55 14.88643071209438 33.89079311034942 33.07360343722529 18.149172740330904 56-57 15.458121117107899 33.852044240593045 32.65409367314944 18.035740969149607 58-59 15.588822279460743 33.127743206724766 32.87751463254643 18.405919881268062 60-61 15.787031615461794 33.10137900257317 33.055992505214384 18.055596876750656 62-63 15.992202013424942 33.02915700093993 33.11327651521145 17.865364470423668 64-65 15.901735534626823 32.893061975783915 33.30468098735393 17.900521502235325 66-67 15.95702929659559 32.88027274787406 32.96699401306769 18.195703942462657 68-69 15.995467665974578 32.52183876031802 33.091735453486606 18.390958120220795 70-71 15.941033552407516 32.52827474966417 32.85156848421008 18.67912321371824 72-73 15.917055150080609 32.1356519492559 32.89792796181401 19.049364938849482 74-75 15.572314028653064 32.22697015137085 32.96634683580215 19.234368984173944 76-77 15.797186994918148 31.74903353616829 33.26377242626755 19.19000704264601 78-79 15.657848905326755 31.797582029246175 33.20567950804618 19.338889557380888 80-81 15.819149636941287 31.85403164919357 32.82679084473856 19.500027869126583 82-83 15.94798837852269 31.426063196058273 32.51486412790466 20.111084297514378 84-85 15.991993333265789 31.13323083313914 32.40420369000699 20.470572143588083 86-87 16.344052520025254 30.899843014924034 32.51782151069164 20.238282954359075 88-89 16.541815724695915 30.647914010853132 32.23237790540459 20.577892359046356 90-91 16.8062428263522 30.679133877002513 32.086168160138975 20.42845513650631 92-93 17.499212142160054 30.50325054895901 31.559742497560183 20.437794811320757 94-95 16.93431323205572 30.279517082515095 31.87449368589065 20.911675999538534 96-97 17.05779283468178 30.118383077706635 31.411424271064813 21.41239981654677 98-99 17.25382323456987 29.974703982095086 31.124811008583663 21.64666177475138 100-101 17.81521158395956 29.572573085813048 31.40425785426505 21.207957475962345 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13376.0 1 9346.0 2 6588.0 3 7925.0 4 6827.5 5 6108.0 6 9170.5 7 16413.0 8 23057.0 9 22281.5 10 15856.0 11 11031.5 12 9281.0 13 8299.0 14 7290.5 15 6078.5 16 5070.0 17 4522.0 18 4263.0 19 4049.0 20 3914.5 21 3976.0 22 4135.5 23 4334.0 24 4609.0 25 5004.5 26 5558.0 27 6261.5 28 6797.5 29 7563.0 30 8685.5 31 9803.0 32 11195.0 33 12460.5 34 13685.5 35 15011.0 36 16417.0 37 17758.0 38 18267.5 39 18265.5 40 18329.0 41 18760.0 42 18940.0 43 19304.0 44 19609.5 45 19420.5 46 19395.0 47 20275.0 48 23621.0 49 24378.0 50 22025.0 51 19637.0 52 17568.0 53 17052.0 54 16312.5 55 15030.0 56 13989.0 57 12749.5 58 11570.5 59 10662.5 60 9586.0 61 8303.5 62 6665.5 63 4997.5 64 3806.0 65 3264.0 66 2759.0 67 2195.0 68 1677.0 69 1170.5 70 893.0 71 724.0 72 618.0 73 533.5 74 433.5 75 357.0 76 254.0 77 174.0 78 136.0 79 109.0 80 71.0 81 57.0 82 48.0 83 33.5 84 21.0 85 10.0 86 7.0 87 6.0 88 4.0 89 2.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12686378569623452 2 0.11321708364922922 3 0.11738690927470306 4 0.13014910285569875 5 0.11839777609299976 6 0.13052817791256002 7 0.15756886530199646 8 0.14644933030073287 9 0.15579984836997726 10-11 0.14954510993176648 12-13 0.1403209502148092 14-15 0.14436441748799594 16-17 0.1400050543340915 18-19 0.15554713166540307 20-21 0.1590851655294415 22-23 0.19263330806166287 24-25 0.21177659843315644 26-27 0.22460197119029568 28-29 0.22068486226939601 30-31 0.21051301491028557 32-33 0.2125979277230225 34-35 0.20267879706848624 36-37 0.20975486479656308 38-39 0.19951983826130906 40-41 0.2051427849380844 42-43 0.19730856709628508 44-45 0.19035885772049532 46-47 0.20507960576194084 48-49 0.24039676522618145 50-51 0.24203942380591356 52-53 0.24361890320950216 54-55 0.24336618650492797 56-57 0.21708364922921405 58-59 0.2175890826383624 60-61 0.19174879959565327 62-63 0.18353550669699267 64-65 0.18580995703816022 66-67 0.19118018701036138 68-69 0.192064695476371 70-71 0.1972453879201415 72-73 0.22630780894617136 74-75 0.22946676775334848 76-77 0.24311346980035378 78-79 0.2394490775840283 80-81 0.252211271165024 82-83 0.2526535253980288 84-85 0.2284559009350518 86-87 0.2318043972706596 88-89 0.23483699772554964 90-91 0.424500884508466 92-93 0.5630528177912559 94-95 0.329921657821582 96-97 0.2650998230983068 98-99 0.2960576194086429 100-101 0.3135582512004043 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 791400.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.575995942988136 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50842860953382 31.809141174743754 2 10.752689022936162 8.94107510387614 3 4.515133960914741 5.631635737231178 4 2.3580622961274273 3.9215515382842887 5 1.3902018476653204 2.8899513189233983 6 0.8695642788700293 2.1691800558280643 7 0.6282339230737289 1.828361572587261 8 0.4751611825465 1.5804239518254986 9 0.3352719058127655 1.2545337056282495 >10 1.9390360978080112 14.752126368127971 >50 0.14565062411598895 4.094901992715215 >100 0.07205219621264869 5.361041738317007 >500 0.006493974765945343 1.885068683562597 >1k 0.002164658255315114 2.5249532039616587 >5k 6.184737872328897E-4 1.6721595483846088 >10k+ 0.0012369475744657794 9.683894306003161 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26357 3.3304270912307303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17181 2.1709628506444276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16730 2.1139752337629516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15053 1.902072276977508 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6860 0.8668182966894111 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6146 0.7765984331564316 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4870 0.6153651756381097 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4755 0.6008339651250948 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2751 0.3476118271417741 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2602 0.32878443265099827 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1801 0.22757139246904218 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1796 0.2269396007076068 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1064 0.1344452868334597 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 967 0.12218852666161233 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 964 0.12180945160475108 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 929 0.11738690927470306 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 926 0.11700783421784179 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.10677280768258782 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.8953752843062926E-4 0.0 16-17 0.0 0.0 0.0 6.317917614354308E-4 0.0 18-19 0.0 0.0 0.0 8.213292898660602E-4 0.0 20-21 0.0 0.0 0.0 0.0024008086934546374 0.0 22-23 0.0 0.0 0.0 0.007139246904220369 0.0 24-25 0.0 0.0 0.0 0.015352539802880972 0.0 26-27 0.0 0.0 0.0 0.02767247915087187 0.0 28-29 0.0 0.0 0.0 0.05079605761940864 0.0 30-31 0.0 0.0 0.0 0.11542835481425323 0.0 32-33 0.0 0.0 0.0 0.20779631033611323 0.0 34-35 0.0 0.0 0.0 0.32145564821834727 0.0 36-37 0.0 0.0 0.0 0.4916603487490523 0.0 38-39 0.0 0.0 0.0 0.7409021986353298 0.0 40-41 0.0 0.0 0.0 1.0170583775587567 0.0 42-43 0.0 0.0 0.0 1.2696487237806418 0.0 44-45 0.0 0.0 0.0 1.541635077078595 0.0 46-47 0.0 0.0 0.0 1.8584154662623198 0.0 48-49 0.0 0.0 0.0 2.2154409906494816 0.0 50-51 0.0 0.0 0.0 2.567791256002022 0.0 52-53 0.0 0.0 0.0 2.8869724538792014 0.0 54-55 0.0 0.0 0.0 3.2172731867576445 0.0 56-57 0.0 0.0 0.0 3.5878822340156686 0.0 58-59 0.0 0.0 0.0 3.974665150366439 0.0 60-61 0.0 0.0 0.0 4.418625221127116 0.0 62-63 0.0 0.0 0.0 4.832132928986606 0.0 64-65 0.0 0.0 0.0 5.2125979277230226 0.0 66-67 0.0 0.0 0.0 5.624147081122063 0.0 68-69 0.0 0.0 0.0 6.081627495577457 0.0 70-71 0.0 0.0 0.0 6.597043214556482 0.0 72-73 0.0 0.0 0.0 7.086681829668941 0.0 74-75 0.0 0.0 0.0 7.54510993176649 0.0 76-77 0.0 0.0 0.0 8.017753348496337 0.0 78-79 0.0 0.0 0.0 8.53512762193581 0.0 80-81 0.0 0.0 0.0 9.070192064695476 0.0 82-83 0.0 0.0 0.0 9.566780389183725 0.0 84-85 0.0 0.0 0.0 10.04157189790245 0.0 86-87 0.0 0.0 0.0 10.51194086429113 0.0 88-89 0.0 0.0 0.0 10.989449077584027 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 75 1.3767749E-7 44.27287 1 GATAGTC 140 0.0 40.658752 7 TAGTCAA 140 0.0 40.65618 9 GTATCAA 12780 0.0 39.640697 1 GGTATCA 7275 0.0 39.578247 1 TATAACG 85 1.6468715E-5 33.48368 2 TATCAAC 16085 0.0 32.321415 2 TACATGG 11435 0.0 32.190403 2 GGGACCG 90 2.4346205E-5 31.623476 7 GTACATG 11510 0.0 31.486101 1 ATACATG 230 0.0 30.93601 1 ATCAACG 16810 0.0 30.929379 3 TCAACGC 16995 0.0 30.564781 4 ACATGGG 11715 0.0 30.248695 3 CAACGCA 17265 0.0 30.029938 5 AACGCAG 17625 0.0 29.551128 6 CATAAGG 65 0.00588026 29.19275 3 TAGATAG 195 1.6370905E-11 29.1909 5 CATGGGA 9095 0.0 29.052322 4 ATGGGAC 2370 0.0 29.021547 5 >>END_MODULE