##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939730_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 852863 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.982857739167954 31.0 31.0 33.0 28.0 34.0 2 30.975026469667462 31.0 31.0 34.0 28.0 34.0 3 30.902949242727143 31.0 31.0 34.0 27.0 34.0 4 34.628900538539014 37.0 35.0 37.0 32.0 37.0 5 34.638036824202715 37.0 35.0 37.0 32.0 37.0 6 34.76543712178861 37.0 35.0 37.0 32.0 37.0 7 34.77340323123409 37.0 35.0 37.0 32.0 37.0 8 34.71358471407483 37.0 35.0 37.0 32.0 37.0 9 36.05579911427744 39.0 35.0 39.0 32.0 39.0 10-11 35.931983214185635 38.0 35.0 39.0 31.0 39.0 12-13 35.81183496059742 38.0 35.0 39.0 30.5 39.0 14-15 36.36270948557975 39.0 36.0 40.0 29.5 41.0 16-17 36.35937600763546 38.5 36.0 40.0 30.5 41.0 18-19 36.61163750801711 39.0 36.0 40.0 31.0 41.0 20-21 36.81259768567754 39.0 36.0 41.0 31.0 41.0 22-23 36.68179297261108 39.0 35.5 41.0 31.0 41.0 24-25 36.398680679077415 39.0 35.0 41.0 30.0 41.0 26-27 36.11322451554352 39.0 35.0 40.5 30.0 41.0 28-29 35.8257522017018 38.0 35.0 40.0 29.5 41.0 30-31 35.337648016152656 37.5 35.0 40.0 28.5 41.0 32-33 34.7143544742825 36.5 34.0 40.0 27.0 41.0 34-35 34.258512211222666 36.0 33.5 40.0 25.0 41.0 36-37 33.882358596867256 35.5 33.0 40.0 23.5 41.0 38-39 33.45986166594166 35.0 33.0 40.0 21.0 41.0 40-41 32.974958463434334 35.0 33.0 39.0 19.5 41.0 42-43 32.40960857722753 35.0 33.0 39.0 16.5 41.0 44-45 31.70970953130808 35.0 31.0 39.0 10.0 40.5 46-47 30.924161911115853 35.0 30.5 39.0 2.0 40.0 48-49 30.329637937159895 35.0 29.5 38.5 2.0 40.0 50-51 29.44989582148598 34.5 26.5 38.0 2.0 39.5 52-53 29.534119782426956 35.0 27.5 38.0 2.0 40.0 54-55 29.581562337679088 35.0 27.5 38.0 2.0 40.0 56-57 29.32785687736483 35.0 27.0 38.0 2.0 40.0 58-59 28.79627970729179 35.0 26.0 38.0 2.0 40.0 60-61 28.31742847327179 34.0 26.0 37.5 2.0 40.0 62-63 27.823880271509022 34.0 24.5 37.0 2.0 39.5 64-65 27.26685939007789 33.0 22.0 36.0 2.0 39.0 66-67 26.70045306221515 33.0 21.5 36.0 2.0 39.0 68-69 26.164295437837026 33.0 20.0 35.0 2.0 38.5 70-71 25.737464282071095 33.0 18.5 35.0 2.0 38.0 72-73 25.320501651496194 33.0 14.0 35.0 2.0 37.5 74-75 24.776617698270414 32.0 7.5 35.0 2.0 37.0 76-77 24.14363619948339 31.0 2.0 35.0 2.0 36.0 78-79 23.52658750584795 31.0 2.0 35.0 2.0 36.0 80-81 22.896600626360858 30.5 2.0 34.0 2.0 35.0 82-83 22.202398861247353 29.5 2.0 34.0 2.0 35.0 84-85 21.500056867281145 29.0 2.0 34.0 2.0 35.0 86-87 20.752100278708305 28.0 2.0 33.5 2.0 35.0 88-89 20.020353210304584 27.0 2.0 33.0 2.0 35.0 90-91 19.298064870911272 26.0 2.0 33.0 2.0 35.0 92-93 18.43443085231743 24.5 2.0 33.0 2.0 35.0 94-95 17.14144299846517 20.5 2.0 32.5 2.0 34.5 96-97 14.657723456170569 2.0 2.0 31.0 2.0 34.0 98-99 13.485849427164737 2.0 2.0 30.5 2.0 34.0 100-101 12.216622716661409 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4678051832403085 1101 2 0.46097620710320086 1101 3 0.8009228992591737 1101 4 0.7325946104781522 1101 5 0.6295217902627428 1101 6 0.7382519695849439 1101 7 0.5595462774608819 1101 8 0.46104368351387137 1101 9 0.752738172251199 1101 10-11 0.7320402637762982 1101 12-13 0.5450354105564301 1101 14-15 0.6956418647446156 1101 16-17 0.42454905062822235 1101 18-19 0.4760716639249267 1101 20-21 0.4896068917726595 1101 22-23 0.5262811558756368 1101 24-25 0.5758947256323523 1101 26-27 0.5601614915292146 1101 28-29 0.9138053757396776 1101 30-31 0.7611116402369191 1101 32-33 0.9061050359351626 1101 34-35 0.6210212741939856 1101 36-37 0.5760611205346251 1101 38-39 0.6687421211199904 1101 40-41 0.7140719812733067 1101 42-43 0.3878738621066731 1101 44-45 0.31190272603330627 1101 46-47 0.32540428741680927 1101 48-49 -0.06989277328815646 1101 50-51 -0.138472706053836 1101 52-53 0.020251068322270527 1101 54-55 -0.0031088381907053986 1101 56-57 -0.04481145997261393 1101 58-59 -0.4426985820981919 1101 60-61 -0.2409726047586105 1101 62-63 -0.3748530538307655 1101 64-65 -0.18820872269849076 1101 66-67 -0.49521323273777895 1101 68-69 -0.5364918650719623 1101 70-71 -0.8635375538912378 1101 72-73 -1.0959716632484202 1101 74-75 -1.1238965547539266 1101 76-77 -1.2921443397383214 1101 78-79 -1.3444951038568753 1101 80-81 -1.6087265894052472 1101 82-83 -1.484512257574071 1101 84-85 -1.3453417200787179 1101 86-87 -1.5533826396343748 1101 88-89 -1.164616028966055 1101 90-91 -1.38204894012598 1101 92-93 -1.0637572313924473 1101 94-95 -0.954533439224992 1101 96-97 0.6104285216324321 1101 98-99 0.4180302621630396 1101 100-101 -0.14289250522733 1102 1 0.7041850901726541 1102 2 0.7756323738295272 1102 3 0.8216045999313302 1102 4 0.9038271602932113 1102 5 1.0953293709014886 1102 6 0.9263122646969038 1102 7 0.8424281929045421 1102 8 0.8191910804036553 1102 9 1.025983210631665 1102 10-11 0.8459785368542896 1102 12-13 0.6887235146012927 1102 14-15 0.8018642725718408 1102 16-17 0.932302699755212 1102 18-19 0.787935801060506 1102 20-21 0.7236939140054872 1102 22-23 0.7577988745249655 1102 24-25 0.8323577570924101 1102 26-27 1.1239943674787796 1102 28-29 1.0573105262785916 1102 30-31 1.117787454578874 1102 32-33 1.0673944604270673 1102 34-35 1.1531614324885453 1102 36-37 0.8240148253431201 1102 38-39 0.954074022643276 1102 40-41 0.9440345951220905 1102 42-43 0.8490479463448324 1102 44-45 0.381993805061267 1102 46-47 0.3733997533516593 1102 48-49 0.5223535775848482 1102 50-51 0.7766746510634519 1102 52-53 0.6437546279611333 1102 54-55 0.03760030550734328 1102 56-57 -0.09040068957926195 1102 58-59 -0.3369335580358275 1102 60-61 -0.11978977057017204 1102 62-63 -0.5055057205769771 1102 64-65 -0.5997825205746423 1102 66-67 -0.48830872651338453 1102 68-69 -0.35169490369105816 1102 70-71 -0.3738704497270575 1102 72-73 -0.16156447238018856 1102 74-75 -0.3553923985275418 1102 76-77 -0.7674774344366462 1102 78-79 -1.1244418087051393 1102 80-81 -1.2689003952358924 1102 82-83 -1.2974025252430046 1102 84-85 -1.2165722812687108 1102 86-87 -1.1563934179822262 1102 88-89 -0.7699594645147165 1102 90-91 -1.1466752774763371 1102 92-93 -1.285166768838259 1102 94-95 -0.9844403715719672 1102 96-97 0.4399039223403829 1102 98-99 0.29201308044248186 1102 100-101 -0.030310474443414392 1103 1 0.6489576924819787 1103 2 0.6493814901597439 1103 3 0.6444779013216362 1103 4 0.6861616194873008 1103 5 0.6651248831681329 1103 6 0.5399744541033371 1103 7 0.5193716118556608 1103 8 0.4855199888423769 1103 9 0.7272844962075524 1103 10-11 0.7151037692267153 1103 12-13 0.4492097293526882 1103 14-15 0.656556231831388 1103 16-17 0.8610344045178664 1103 18-19 0.5801110687797291 1103 20-21 0.5401812284348324 1103 22-23 0.6457285998673044 1103 24-25 0.5940614724329123 1103 26-27 0.8999035654993577 1103 28-29 1.0101775437948888 1103 30-31 0.8519952937117026 1103 32-33 0.8233562858924657 1103 34-35 0.8581440209209745 1103 36-37 0.576978719250377 1103 38-39 0.47801558261500077 1103 40-41 0.24073948868897332 1103 42-43 0.1864448515368835 1103 44-45 0.40919643381522874 1103 46-47 0.03869836828109996 1103 48-49 -0.054783594148194226 1103 50-51 -0.08267915722602126 1103 52-53 0.09919148811875189 1103 54-55 0.07171519567046403 1103 56-57 -0.03960604134529433 1103 58-59 -0.32038602314723974 1103 60-61 -0.11712305153788805 1103 62-63 -0.272668551462214 1103 64-65 -0.35478474611335287 1103 66-67 -0.6533088574274295 1103 68-69 -0.5716069294549442 1103 70-71 -0.5407910896348938 1103 72-73 -0.4548263266466748 1103 74-75 -0.6751172172115929 1103 76-77 -0.8540037612504605 1103 78-79 -0.8923652501734729 1103 80-81 -0.9215068938089317 1103 82-83 -0.6429632300842059 1103 84-85 -0.9572158546983935 1103 86-87 -1.0559692564866658 1103 88-89 -0.9695274481766454 1103 90-91 -0.8917201809926887 1103 92-93 -1.0741555887790852 1103 94-95 -0.9880221797567152 1103 96-97 0.5527518264065723 1103 98-99 0.7891447537857648 1103 100-101 0.552213810111347 1104 1 0.5614080377189268 1104 2 0.4599653743671226 1104 3 0.6152456413259983 1104 4 0.7731075535041754 1104 5 0.7612331673328896 1104 6 0.8002364384674792 1104 7 0.7018347925000654 1104 8 0.6289112759851534 1104 9 0.8991445141669772 1104 10-11 0.6837534133358645 1104 12-13 0.7001089688674753 1104 14-15 0.7987667122206119 1104 16-17 0.9169825758527992 1104 18-19 0.71455562032682 1104 20-21 0.6630764499090205 1104 22-23 0.6604804402009279 1104 24-25 0.8980209166039614 1104 26-27 0.996342881417803 1104 28-29 1.0199055650620963 1104 30-31 0.9192581557539015 1104 32-33 1.157952021290292 1104 34-35 1.0723279071576641 1104 36-37 1.030148506831999 1104 38-39 1.053820585887273 1104 40-41 1.0069790694328162 1104 42-43 0.8120999392793209 1104 44-45 0.7281448943273254 1104 46-47 0.6910084879598593 1104 48-49 0.3257583340039645 1104 50-51 0.5536298260372128 1104 52-53 0.7924823242466239 1104 54-55 0.7314589441167669 1104 56-57 0.7376527113372049 1104 58-59 0.8037686409760205 1104 60-61 0.8962957835347396 1104 62-63 0.44077336705070636 1104 64-65 0.6233557432555799 1104 66-67 0.6600260148019252 1104 68-69 0.34954156048219787 1104 70-71 0.4758543537954587 1104 72-73 0.3117721889105489 1104 74-75 0.018478015931151504 1104 76-77 -0.012349820070109985 1104 78-79 -0.13037581274988952 1104 80-81 -0.19591572943961566 1104 82-83 -0.029482754616068974 1104 84-85 -0.3730285186288711 1104 86-87 -0.3773249900581028 1104 88-89 -0.3749398807653854 1104 90-91 -0.2263222579949904 1104 92-93 -0.1925305145823124 1104 94-95 0.4659973828247743 1104 96-97 1.7147164047406083 1104 98-99 2.03080554389895 1104 100-101 1.7178489091164764 1105 1 0.4824226380653762 1105 2 0.6012645577375757 1105 3 0.40083831640642487 1105 4 0.5200340568449349 1105 5 0.5437201754991889 1105 6 0.6679597742754453 1105 7 0.37307953390071447 1105 8 0.38267767044197143 1105 9 0.5915770803634643 1105 10-11 0.35025056750562555 1105 12-13 0.1929189169001404 1105 14-15 0.3962512457519125 1105 16-17 0.41588507523413654 1105 18-19 0.36222698879997495 1105 20-21 0.39428778373729045 1105 22-23 0.29086400862458106 1105 24-25 0.36517384904642824 1105 26-27 0.6244732946818488 1105 28-29 0.41641014313683655 1105 30-31 0.7887644660731326 1105 32-33 0.8400381385879854 1105 34-35 0.6616671796149802 1105 36-37 0.5687466261094016 1105 38-39 0.6405491184287158 1105 40-41 0.19322999742094282 1105 42-43 0.36224718006130274 1105 44-45 0.40664897747506146 1105 46-47 0.6213116307462947 1105 48-49 0.4126600909948088 1105 50-51 0.3659275246266702 1105 52-53 0.4335055858155421 1105 54-55 0.20809838979852913 1105 56-57 0.13153417632112152 1105 58-59 0.042491729320492055 1105 60-61 0.25760486544515615 1105 62-63 0.23920692310118596 1105 64-65 0.2226121154406826 1105 66-67 0.3320935723371896 1105 68-69 0.04184915761161889 1105 70-71 0.02714487594344206 1105 72-73 0.06322479965046668 1105 74-75 -0.0049134713244747275 1105 76-77 -0.25985291336385075 1105 78-79 -0.6012617320767895 1105 80-81 -0.40690805807114216 1105 82-83 -0.4760232148981771 1105 84-85 -0.8533155762878657 1105 86-87 -0.6953972493998535 1105 88-89 -0.6653234491890458 1105 90-91 -0.8470201050625441 1105 92-93 -0.5069675351217846 1105 94-95 0.05634506904105763 1105 96-97 1.0499613936047503 1105 98-99 1.2874920076455467 1105 100-101 1.0997853109971985 1106 1 0.6804495895019365 1106 2 0.7379617354644985 1106 3 0.6388144160758884 1106 4 0.6268761621080898 1106 5 0.6778696796944601 1106 6 0.6807718769960118 1106 7 0.5583070955584759 1106 8 0.550333403978712 1106 9 0.8324720714644016 1106 10-11 0.7390517370962684 1106 12-13 0.7866679634295082 1106 14-15 1.0586832315134274 1106 16-17 0.7320317825822187 1106 18-19 0.945275552237554 1106 20-21 0.871792233266909 1106 22-23 0.4726743315332982 1106 24-25 0.7684423463767089 1106 26-27 0.9999945252946105 1106 28-29 0.9761419000653859 1106 30-31 0.908768279955666 1106 32-33 0.9233638441562491 1106 34-35 1.0272888424677618 1106 36-37 1.074931472282806 1106 38-39 0.8665572547114522 1106 40-41 1.1219561606551167 1106 42-43 1.110623737396672 1106 44-45 1.152808397764911 1106 46-47 0.6174685084477964 1106 48-49 0.5802903986479997 1106 50-51 0.8496079212704544 1106 52-53 0.7352154764842389 1106 54-55 0.5641751759668487 1106 56-57 0.630192960963889 1106 58-59 0.3430027895798382 1106 60-61 0.46364351278815263 1106 62-63 0.6466287385092713 1106 64-65 0.9809429061856605 1106 66-67 0.6533559674554219 1106 68-69 0.38395569206369373 1106 70-71 0.5096785652391453 1106 72-73 0.49462322324568575 1106 74-75 0.36873895684740177 1106 76-77 0.16789435335366676 1106 78-79 -0.20524088285227648 1106 80-81 0.00913821703691653 1106 82-83 -0.01616814243440956 1106 84-85 0.08017539752347247 1106 86-87 0.12745807732274983 1106 88-89 0.190440598611616 1106 90-91 0.13642001698169182 1106 92-93 0.2587507460884879 1106 94-95 0.3409916492597205 1106 96-97 1.8283938878839248 1106 98-99 1.905274870600035 1106 100-101 1.5972389087597207 1107 1 0.56353017647195 1107 2 0.6126579125071352 1107 3 0.6528497490609446 1107 4 0.3471616023969162 1107 5 0.6376215688539588 1107 6 0.6144299421925581 1107 7 0.6949802127145759 1107 8 0.5563718469335797 1107 9 0.5972290989415256 1107 10-11 0.6069368840472507 1107 12-13 0.5639960873181522 1107 14-15 0.786313768081321 1107 16-17 0.6484556361487464 1107 18-19 0.4665106615402408 1107 20-21 0.5693771013507174 1107 22-23 0.7677057378135785 1107 24-25 0.5193056818438535 1107 26-27 0.6922742950391125 1107 28-29 0.6155080352411559 1107 30-31 0.917355034133152 1107 32-33 0.9790735083629087 1107 34-35 0.854850459357742 1107 36-37 0.801499394955421 1107 38-39 0.6674051384358606 1107 40-41 0.6813650456524556 1107 42-43 0.47080202107595426 1107 44-45 0.8462470482146109 1107 46-47 0.7778514664018878 1107 48-49 0.559898390387449 1107 50-51 0.5329318118721176 1107 52-53 0.6095952606994253 1107 54-55 0.7076875287767379 1107 56-57 0.7171629723196915 1107 58-59 0.6412698643687236 1107 60-61 0.815232589632366 1107 62-63 0.7192605136692443 1107 64-65 0.2967618117607991 1107 66-67 0.18983740942186245 1107 68-69 0.059985992195404236 1107 70-71 0.3976932860899218 1107 72-73 0.5257364109402118 1107 74-75 0.4711404765140337 1107 76-77 0.49898595766151743 1107 78-79 0.5893956455580813 1107 80-81 0.8304731495029891 1107 82-83 0.7526584571275485 1107 84-85 0.6698212582959115 1107 86-87 0.5563426577098305 1107 88-89 0.3956483264451087 1107 90-91 0.23590593995352904 1107 92-93 0.3469838760965054 1107 94-95 0.648447605661282 1107 96-97 1.9037395286529826 1107 98-99 2.1227796103608014 1107 100-101 1.869776379235855 1108 1 0.5121777295838079 1108 2 0.6142034595163821 1108 3 0.6006269217170725 1108 4 0.5628878851511985 1108 5 0.5532406704721211 1108 6 0.587802517250438 1108 7 0.4452630863626865 1108 8 0.48736186739995446 1108 9 0.5146809731193258 1108 10-11 0.46478137441462763 1108 12-13 0.38163894783594543 1108 14-15 0.47423139527499103 1108 16-17 0.5843601976883761 1108 18-19 0.34996609681749646 1108 20-21 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2311 64-65 1.1548649588352617 2311 66-67 1.2279409664129268 2311 68-69 1.015069380083812 2311 70-71 1.2261618738187074 2311 72-73 1.168202927384499 2311 74-75 1.37672944831057 2311 76-77 1.2397733833490605 2311 78-79 1.293795135814726 2311 80-81 0.885376656339556 2311 82-83 1.2430019069615916 2311 84-85 1.1254670758806107 2311 86-87 1.166480015941982 2311 88-89 1.2617906615471135 2311 90-91 1.6989603973746483 2311 92-93 1.6671094552544155 2311 94-95 1.9579454939879426 2311 96-97 3.4785047006913654 2311 98-99 3.471660236616927 2311 100-101 3.088948540593499 2312 1 0.1045923668492783 2312 2 -0.37272494357476305 2312 3 -0.7026962417738005 2312 4 -0.24438664056788895 2312 5 -1.7039776122773418 2312 6 -0.35755828596702344 2312 7 0.12136099871761274 2312 8 0.24392376468018995 2312 9 0.24801752530784427 2312 10-11 0.10972250851300203 2312 12-13 0.3326958197872827 2312 14-15 0.40246484405213323 2312 16-17 -0.34264073403969775 2312 18-19 -0.17163002907279434 2312 20-21 -0.005546611963247017 2312 22-23 0.028249335458724545 2312 24-25 0.08309473393581612 2312 26-27 0.17274538567046704 2312 28-29 0.06704443884986944 2312 30-31 0.0559279456381887 2312 32-33 -0.722474047388765 2312 34-35 -0.48427957704043934 2312 36-37 0.014319054555848254 2312 38-39 -0.22542638463340126 2312 40-41 -0.14045204232408537 2312 42-43 -0.03362146135852129 2312 44-45 -0.09659249234121248 2312 46-47 -0.3100913188849965 2312 48-49 -0.17053200999256646 2312 50-51 -0.04063977946031727 2312 52-53 -0.22989586400323248 2312 54-55 -0.3069434799677495 2312 56-57 -0.0969591370147107 2312 58-59 0.09417526887804684 2312 60-61 -0.1375082454159866 2312 62-63 0.029461891230166515 2312 64-65 -0.049297020917272505 2312 66-67 -0.056086030212494364 2312 68-69 -0.005740518678841511 2312 70-71 0.11648808304255454 2312 72-73 0.15357975528101164 2312 74-75 0.3322975023038204 2312 76-77 0.2881423372298251 2312 78-79 0.6813091965571338 2312 80-81 0.5653541591517062 2312 82-83 0.7767476887388689 2312 84-85 0.7638247811674503 2312 86-87 0.9589434580117207 2312 88-89 0.9428930237518394 2312 90-91 0.9334935807267293 2312 92-93 0.9095166543545297 2312 94-95 1.7228498809395703 2312 96-97 3.1061762417487326 2312 98-99 3.565023566200729 2312 100-101 3.092885548467514 2313 1 0.012191156583892138 2313 2 -0.29220766448464985 2313 3 -0.8263839058157956 2313 4 -0.04283631352544148 2313 5 -0.2272520467230308 2313 6 0.12411718168284835 2313 7 0.11266286723404306 2313 8 0.2497147074790078 2313 9 0.26694745073383075 2313 10-11 -0.5655596176795541 2313 12-13 0.18565039838161823 2313 14-15 0.3525706901963588 2313 16-17 -0.447263072914005 2313 18-19 0.051509396207379154 2313 20-21 -2.0713372162627124 2313 22-23 -0.3671452506346782 2313 24-25 -0.02320578058319711 2313 26-27 0.037551998385552565 2313 28-29 -0.2978259679742834 2313 30-31 -0.162277200593536 2313 32-33 -0.24993871326386596 2313 34-35 -0.5618050426072472 2313 36-37 -1.5306746701868903 2313 38-39 -0.6058397704601717 2313 40-41 -0.32883018776423967 2313 42-43 -0.20671115327202472 2313 44-45 0.02083879228987584 2313 46-47 -0.13380410999444692 2313 48-49 -0.5736593534496386 2313 50-51 -0.1762946975955515 2313 52-53 -0.1914944141844579 2313 54-55 -0.3704895731644342 2313 56-57 -0.1730954533085054 2313 58-59 -0.10128141882765718 2313 60-61 -0.5337145789992874 2313 62-63 -0.6217652991210336 2313 64-65 -0.42250362530005603 2313 66-67 -0.08549902025540135 2313 68-69 -0.561692509055046 2313 70-71 -0.0487347536861904 2313 72-73 -0.13524742257175504 2313 74-75 0.24693838052737505 2313 76-77 0.15118875330281867 2313 78-79 -0.10600710244715827 2313 80-81 0.05429564563256051 2313 82-83 0.1905045628795996 2313 84-85 0.22811960889731608 2313 86-87 0.46006571356310744 2313 88-89 0.6081256248850302 2313 90-91 0.5204104504930065 2313 92-93 0.8316435759893253 2313 94-95 1.4743080320040214 2313 96-97 3.1137345417528994 2313 98-99 2.9777929335714717 2313 100-101 2.18066494062683 2314 1 -0.014749079106341156 2314 2 -0.08752332105030547 2314 3 -0.8486566330885239 2314 4 -0.05481442800355296 2314 5 -0.21355255177353882 2314 6 -0.417595781280113 2314 7 -0.2192436984225239 2314 8 -1.3695445517802511 2314 9 -1.4401400913537117 2314 10-11 -0.7016181193630615 2314 12-13 -0.1842022952210698 2314 14-15 -0.14713259263191958 2314 16-17 -0.21113512678606128 2314 18-19 -1.5148437182707326 2314 20-21 -1.197883933434433 2314 22-23 -1.434792557031983 2314 24-25 -1.3067958479232686 2314 26-27 -1.4755772103686624 2314 28-29 -2.473943392216704 2314 30-31 -1.3956294733208097 2314 32-33 -1.0932951947453446 2314 34-35 -1.4862116082638153 2314 36-37 -3.1579705456077676 2314 38-39 -3.333491285611686 2314 40-41 -4.115557881366939 2314 42-43 -3.2916417088275836 2314 44-45 -3.2210782952522123 2314 46-47 -2.8686378642031976 2314 48-49 -1.271784353449636 2314 50-51 -0.6172542935551455 2314 52-53 -1.6279590606491077 2314 54-55 -1.2204874687873257 2314 56-57 -1.2293244095374618 2314 58-59 -2.633871907043144 2314 60-61 -1.3161325082922168 2314 62-63 -1.6865506526563898 2314 64-65 -0.9367313189027513 2314 66-67 -2.056542791299176 2314 68-69 -1.5859791252166637 2314 70-71 -0.8218955280969595 2314 72-73 -0.49485600843034305 2314 74-75 -0.8934488248598313 2314 76-77 -0.4678327113436467 2314 78-79 0.07035442953937476 2314 80-81 0.048142446979358766 2314 82-83 -0.09602321489817811 2314 84-85 2.1977724748367677E-4 2314 86-87 0.10197648797388226 2314 88-89 0.2845755407099446 2314 90-91 0.8181314100889701 2314 92-93 0.7971738790196277 2314 94-95 1.4142743619703495 2314 96-97 2.9667795754229314 2314 98-99 2.814259684413223 2314 100-101 2.258017634229521 2315 1 -1.7953887672870152 2315 2 -1.314796812586092 2315 3 -0.8390467893803191 2315 4 -1.1295119708978163 2315 5 -1.3371415311002224 2315 6 -1.873064465601253 2315 7 -1.3080189668699234 2315 8 -0.5866778010446438 2315 9 -0.410992626796407 2315 10-11 -3.241833641545796 2315 12-13 -1.2526275311872936 2315 14-15 -0.7606837409371678 2315 16-17 -1.5444210758097157 2315 18-19 -0.9862289450657968 2315 20-21 -0.6077123563776112 2315 22-23 -0.839675433718142 2315 24-25 -2.7974091690283984 2315 26-27 -3.4865222216415432 2315 28-29 -5.393198235733841 2315 30-31 -8.076546588992024 2315 32-33 -6.493143870659598 2315 34-35 -6.056651441654466 2315 36-37 -3.322955069280816 2315 38-39 -5.79791101888388 2315 40-41 -3.3386783674382414 2315 42-43 -3.465588745397568 2315 44-45 -2.7561778405156936 2315 46-47 -1.862047623842468 2315 48-49 -2.6519945257909576 2315 50-51 -2.5088749133724875 2315 52-53 -1.7776300454020593 2315 54-55 -3.3290250514232724 2315 56-57 -2.8898998996211525 2315 58-59 -1.8843346683255753 2315 60-61 -1.9941255534983782 2315 62-63 -2.167026788542362 2315 64-65 -1.3802553358693928 2315 66-67 -2.148854304922164 2315 68-69 -1.4613461625509139 2315 70-71 -1.5937889898264928 2315 72-73 -1.0310851344955339 2315 74-75 -1.1845512743229385 2315 76-77 -0.6406333533246169 2315 78-79 -0.5019272778243007 2315 80-81 -0.1850235127439781 2315 82-83 0.1669696392422182 2315 84-85 -0.27269626845544437 2315 86-87 -0.8039778855214728 2315 88-89 -1.3142543725461984 2315 90-91 -0.9133042580680097 2315 92-93 0.24933483281992963 2315 94-95 0.42857219528458046 2315 96-97 1.818025706341194 2315 98-99 1.9186549612675172 2315 100-101 1.7446522018476962 2316 1 -2.91050034994214 2316 2 -1.8149599762893018 2316 3 -1.8771533033017107 2316 4 -2.1704340835972005 2316 5 -1.7847627026976056 2316 6 -4.773677272394114 2316 7 -2.4389418502923235 2316 8 -3.8569654447147954 2316 9 -5.155745560873353 2316 10-11 -4.895778185564737 2316 12-13 -2.759406614092491 2316 14-15 -3.94437655369903 2316 16-17 -3.147438866392797 2316 18-19 -1.9779969672757431 2316 20-21 -2.039547644669021 2316 22-23 -2.7286822997123608 2316 24-25 -2.173796675444251 2316 26-27 -2.990289745341272 2316 28-29 -2.7575206472113862 2316 30-31 -2.6683118234804013 2316 32-33 -2.3698101492316894 2316 34-35 -4.411605468846229 2316 36-37 -4.794781043302535 2316 38-39 -4.495062787365242 2316 40-41 -3.563393322908489 2316 42-43 -3.48932701441926 2316 44-45 -2.7905963627936856 2316 46-47 -2.7944888316146326 2316 48-49 -3.4699464687508943 2316 50-51 -4.391599566834099 2316 52-53 -2.3110941830039913 2316 54-55 -2.548995960728849 2316 56-57 -2.0914357702183963 2316 58-59 -2.32935915927942 2316 60-61 -2.511691479013738 2316 62-63 -1.5560639915065302 2316 64-65 -2.12935219607499 2316 66-67 -2.7519007926389705 2316 68-69 -1.9501411617063944 2316 70-71 -1.4170777797421543 2316 72-73 -1.3092603903509819 2316 74-75 -2.5099669318070674 2316 76-77 -2.5139043510173664 2316 78-79 -2.624618183456647 2316 80-81 -2.7598238216891176 2316 82-83 -1.7233400766233622 2316 84-85 -1.374116038648797 2316 86-87 -1.0374749838312916 2316 88-89 -1.2842166727116577 2316 90-91 -0.9411986919719517 2316 92-93 -0.8962768834818498 2316 94-95 -0.5053071488465299 2316 96-97 0.9302929930845938 2316 98-99 1.4883488202448838 2316 100-101 0.9517135005651802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7622.0 3 1465.0 4 1189.0 5 1239.0 6 1468.0 7 1742.0 8 2417.0 9 3084.0 10 3819.0 11 5416.0 12 8439.0 13 10788.0 14 12628.0 15 15932.0 16 19586.0 17 22059.0 18 16875.0 19 12913.0 20 13104.0 21 13972.0 22 15273.0 23 16728.0 24 18126.0 25 20332.0 26 22756.0 27 26300.0 28 29641.0 29 34245.0 30 39680.0 31 47369.0 32 57585.0 33 68745.0 34 79455.0 35 81721.0 36 63464.0 37 45020.0 38 10558.0 39 108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.58064671378449 20.068739897664887 13.740701743265404 16.609911645285223 2 15.671496382408314 24.725922239385504 39.38867160906534 20.21390976914084 3 19.668961631244386 33.827895265094824 28.579411056916292 17.923732046744494 4 10.997670661607243 19.131570049216666 36.05872186948191 33.81203741969418 5 10.944312826810238 43.06346370131745 29.550454687400947 16.441768784471364 6 25.272101216604426 37.7776629877776 19.05289640606858 17.897339389549387 7 24.706945944676654 40.325928708965385 19.43619969003898 15.530925656318978 8 27.870353737614955 31.452462215271687 20.16570920699327 20.511474840120083 9 30.83724234294001 17.031344484040087 20.22238791073955 31.909025262280355 10-11 24.103493155228577 30.821401923310614 23.89015725450668 21.18494766695413 12-13 26.793422459233796 30.07473730989126 26.68528788628611 16.446552344588838 14-15 23.486299062107083 28.757236121497577 29.456165334003288 18.300299482392056 16-17 17.555507029951258 33.55152245170137 28.70927371258441 20.183696805762963 18-19 16.744329953801255 29.127108489261683 37.08023223250492 17.048329324432142 20-21 18.92478687893032 28.081534518683476 37.21156127480344 15.782117327582764 22-23 17.90651682700638 29.506834236792162 36.90740432920164 15.679244606999815 24-25 16.88636853635024 30.231972041397437 37.21151829569547 15.670141126556855 26-27 16.356812023997236 30.669029888449977 37.30005240289602 15.67410568465676 28-29 15.915648104902317 31.378282236341583 37.41627525107878 15.289794407677327 30-31 15.696109723664012 31.987284443888292 36.97028552427582 15.346320308171876 32-33 15.493030562595688 32.58081269174325 36.446051043800004 15.480105701861063 34-35 15.967477822050144 32.41962842536556 36.15317116490621 15.459722587678081 36-37 15.717112182952475 32.81637509462462 35.832676075196986 15.633836647225921 38-39 15.549501958510934 33.04394711756176 35.96006975341381 15.4464811705135 40-41 15.18565914030718 33.191779751945504 36.09328559154461 15.529275516202706 42-43 15.065146340675572 33.5863229390881 35.315277749228436 16.033252971007897 44-45 15.1310816549114 33.51663680129869 34.96033514992297 16.39194639386694 46-47 15.215289363001949 33.70468056110341 34.35765642989732 16.722373645997322 48-49 15.389857792896402 33.91825955660524 33.62820524118806 17.06367740931029 50-51 15.286300461091612 34.24446963300116 33.299076112728 17.170153793179228 52-53 15.445765444266756 34.252293138539244 32.83553405282079 17.466407364373204 54-55 14.958286122642795 34.05287518982224 32.86939524868476 18.119443438850205 56-57 15.467690991643087 34.00659434662857 32.470400642984046 18.0553140187443 58-59 15.638119252203358 33.22833035870598 32.75405452086567 18.37949586822499 60-61 15.800618266164582 33.230191077490026 32.868488337465486 18.100702318879907 62-63 16.040802686192503 33.132154727413365 32.925416105525976 17.90162648086816 64-65 15.8828669685979 33.04402824497773 33.0551275064364 18.017977279987974 66-67 15.965876792836639 33.00992196785696 32.80227472388084 18.22192651542556 68-69 16.0216494173321 32.59028959266983 32.86836574031388 18.51969524968419 70-71 15.922578583958845 32.521422912458966 32.72515420797036 18.830844295611822 72-73 15.911253936175212 32.21530290924473 32.7130234525544 19.16041970202566 74-75 15.57111112939028 32.418663241672434 32.69063770932751 19.319587919609777 76-77 15.780114754213962 31.955710796815467 32.923435292679656 19.340739156290915 78-79 15.690485605983595 31.95364111941513 32.939507701311456 19.41636557328982 80-81 15.869558319397257 32.02146175442967 32.55759429034127 19.5513856358318 82-83 15.911092726030237 31.629161689278163 32.267168391345244 20.192577193346352 84-85 15.988089775549378 31.284667407334727 32.164656267937744 20.562586549178146 86-87 16.39974010348863 31.10797924587714 32.129471230443144 20.36280942019109 88-89 16.4835093820865 30.839511685015687 31.94579891668331 20.731180016214502 90-91 16.44749442180004 30.975195075439355 31.90101764696897 20.676292855791633 92-93 17.087042096295644 30.73505478687465 31.5699303780735 20.607972738756207 94-95 16.92020336206638 30.499257045727084 31.457136326968037 21.123403265238494 96-97 17.162186083382718 30.085070793985523 31.264991931614983 21.48775119101677 98-99 17.408572485063612 29.95120064289817 30.78504003710656 21.85518683493166 100-101 17.716947220322858 29.677052178805702 31.047709309920908 21.558291290950535 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14725.0 1 9991.5 2 6914.5 3 8582.5 4 7033.5 5 6263.0 6 10154.0 7 18354.0 8 25519.5 9 24352.0 10 16727.0 11 11162.5 12 9491.0 13 8473.5 14 7563.5 15 6518.0 16 5460.0 17 4869.0 18 4513.5 19 4298.5 20 4200.5 21 4312.5 22 4476.0 23 4670.0 24 4945.0 25 5298.0 26 5773.0 27 6377.0 28 7232.0 29 8303.0 30 9392.0 31 10637.0 32 12195.0 33 13671.0 34 15107.5 35 16360.0 36 17808.0 37 19373.0 38 20029.5 39 19916.5 40 19907.5 41 20311.5 42 20737.0 43 21125.5 44 21165.5 45 20923.5 46 20759.0 47 21868.0 48 25545.5 49 25979.0 50 23693.0 51 21410.0 52 18718.5 53 18222.0 54 17755.5 55 16142.0 56 14824.5 57 13689.5 58 12430.5 59 11351.0 60 10298.5 61 8823.5 62 7010.0 63 5444.0 64 4197.5 65 3502.0 66 2954.0 67 2363.0 68 1831.0 69 1311.0 70 955.5 71 788.5 72 660.5 73 580.5 74 498.0 75 393.0 76 275.0 77 180.5 78 141.5 79 109.0 80 85.0 81 65.5 82 47.0 83 40.0 84 26.5 85 14.5 86 11.0 87 8.0 88 3.5 89 1.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11209303252691229 2 0.10728569535787108 3 0.11701762182202768 4 0.131205129077003 5 0.1253425227732942 6 0.12499076639507166 7 0.13401918010278321 8 0.13249490246381893 9 0.1404680470368629 10-11 0.13536757955263623 12-13 0.13442956254404284 14-15 0.12428725363862661 16-17 0.12651504403403596 18-19 0.14263721136923516 20-21 0.17224337320296462 22-23 0.1873102714034962 24-25 0.20489809031462264 26-27 0.20695000252092072 28-29 0.20935367110544134 30-31 0.20865015834899628 32-33 0.2098813056727751 34-35 0.18373408155823384 36-37 0.17265375564422422 38-39 0.18508248100808689 40-41 0.17324001627459512 42-43 0.1771093364350429 44-45 0.18086140446941656 46-47 0.19733532818283828 48-49 0.2128712348876666 50-51 0.22834851552945784 52-53 0.24839862908814192 54-55 0.24236014459532187 56-57 0.21468864284181632 58-59 0.20823977590773668 60-61 0.1889518011685347 62-63 0.18133041297371325 64-65 0.17060184343792614 66-67 0.1663807668992558 68-69 0.17324001627459512 70-71 0.17546780667000444 72-73 0.20906054079025588 74-75 0.21855796300226413 76-77 0.23462150427442627 78-79 0.2270587421426419 80-81 0.22911065434893998 82-83 0.22647248151227103 84-85 0.21451276465270508 86-87 0.20577748126017892 88-89 0.2078880195295141 90-91 0.2089432886641817 92-93 0.21550940772433555 94-95 0.21908559756959792 96-97 8.591884042337398 98-99 0.27431134895053483 100-101 0.285098544549359 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 852863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.7901662978291 #Duplication Level Percentage of deduplicated Percentage of total 1 76.74714506546266 31.305288101038357 2 10.325621965463423 8.4236767419954 3 4.360933457575926 5.336496028448667 4 2.3466408608367972 3.828794838192552 5 1.4366559163574422 2.9300716870490064 6 0.9773646067037048 2.3920118904633876 7 0.6841554569299837 1.9534770403218902 8 0.47674998888723974 1.555736906335895 9 0.37817041961059855 1.3883070874352512 >10 2.020836739783963 15.196884443696737 >50 0.1616995817728968 4.538819141760512 >100 0.07305348101617119 5.310988585868129 >500 0.006063727673260241 1.6376294087149876 >1k 0.002598740431397246 1.7529371756260907 >5k 0.001154995747287665 2.842766355634706 >10k+ 0.001154995747287665 9.60611456741842 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28122 3.2973642894579784 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18670 2.1890971938048667 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18077 2.1195666830428803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16690 1.9569379841779981 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7174 0.8411667524561388 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6679 0.7831269500494218 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5191 0.6086557864510478 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5092 0.5970478259697044 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2922 0.3426107123887424 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2772 0.325022893477616 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1965 0.23040042773575595 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1871 0.21937872788478338 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1118 0.13108787695092883 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1083 0.12698405253833264 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1075 0.12604603552973923 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 1072 0.12569427915151674 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1005 0.11783838670454691 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 944 0.11068600701402219 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 910 0.10669943472750018 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 905 0.10611317409712931 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1725212607417604E-4 0.0 14-15 0.0 0.0 0.0 1.1725212607417604E-4 0.0 16-17 0.0 0.0 0.0 5.276345673337922E-4 0.0 18-19 0.0 0.0 0.0 7.035127564450562E-4 0.0 20-21 0.0 0.0 0.0 0.001993286143260993 0.0 22-23 0.0 0.0 0.0 0.005745354177634626 0.0 24-25 0.0 0.0 0.0 0.013835750876752772 0.0 26-27 0.0 0.0 0.0 0.02585409379935582 0.0 28-29 0.0 0.0 0.0 0.05018390995974735 0.0 30-31 0.0 0.0 0.0 0.11783838670454691 0.0 32-33 0.0 0.0 0.0 0.20595335944929022 0.0 34-35 0.0 0.0 0.0 0.32039143449768603 0.0 36-37 0.0 0.0 0.0 0.4912864082507976 0.0 38-39 0.0 0.0 0.0 0.742616340490794 0.0 40-41 0.0 0.0 0.0 1.0245490776361503 0.0 42-43 0.0 0.0 0.0 1.2777550438933334 0.0 44-45 0.0 0.0 0.0 1.5535320444197955 0.0 46-47 0.0 0.0 0.0 1.8732199661610363 0.0 48-49 0.0 0.0 0.0 2.2089714291744396 0.0 50-51 0.0 0.0 0.0 2.567000796141936 0.0 52-53 0.0 0.0 0.0 2.8978276698602237 0.0 54-55 0.0 0.0 0.0 3.2154050533321294 0.0 56-57 0.0 0.0 0.0 3.575838088884147 0.0 58-59 0.0 0.0 0.0 3.9774266206882 0.0 60-61 0.0 0.0 0.0 4.4095007052715385 0.0 62-63 0.0 0.0 0.0 4.814782679046928 0.0 64-65 0.0 0.0 0.0 5.192158646816663 0.0 66-67 0.0 0.0 0.0 5.613680040053326 0.0 68-69 0.0 0.0 0.0 6.063283317484754 0.0 70-71 0.0 0.0 0.0 6.565884555901709 0.0 72-73 0.0 0.0 0.0 7.061509292817252 0.0 74-75 0.0 0.0 0.0 7.535442386409072 0.0 76-77 0.0 0.0 0.0 8.007733950235853 0.0 78-79 0.0 0.0 0.0 8.530502554337566 0.0 80-81 0.0 0.0 0.0 9.065699883803143 0.0 82-83 0.0 0.0 0.0 9.60974974878732 0.0 84-85 0.0 0.0 0.0 10.153271979204163 0.0 86-87 0.0 0.0 0.0 10.622045979248718 0.0 88-89 0.0 0.0 0.0 11.070711239671553 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGTC 185 0.0 50.859333 7 TAGATAG 225 0.0 48.0875 5 TAGTCAA 215 0.0 43.762684 9 GGTATCA 8210 0.0 42.2216 1 GTATCAA 14035 0.0 38.73966 1 ATAACGC 80 1.1516731E-5 35.279514 3 GTTTACG 55 0.0027070474 34.206425 1 AGATAGT 285 0.0 33.013954 6 ATATGGG 195 0.0 31.359571 3 GTACATG 12985 0.0 30.715971 1 TACATGG 12660 0.0 30.579298 2 TATCAAC 17840 0.0 30.48066 2 ATCAACG 18420 0.0 29.520899 3 GTACACG 80 4.7597612E-4 29.396141 1 ATAGTCA 340 0.0 29.057133 8 TCAACGC 18705 0.0 28.995659 4 GGGACCG 65 0.006124658 28.950695 7 CAACGCA 18940 0.0 28.56306 5 CCTCTAG 330 0.0 28.50535 1 ACATGGG 12910 0.0 28.456808 3 >>END_MODULE