FastQCFastQC Report
Fri 27 May 2016
SRR939730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852863
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300773.5265921959329924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225142.6398143664339995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214472.5147063479128535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210642.469798783626444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89671.0513998145071364No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71450.8377664407999879No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66340.7778506043760839No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59640.699291679906386No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37270.4369986738784541No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35400.41507252630258323No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.28023258131728074No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13960.16368396799954976No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.16063541272162116No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.16004915209125029No Hit
GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA13510.15840762232621183No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12510.14668240971879423No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11170.13097062482485464No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT10460.12264572387358813RNA PCR Primer, Index 39 (95% over 23bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9990.11713487394810188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9850.11549334418306341No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9050.10611317409712931No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.10411988795386834No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.10318187094527492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97750.041.497111
TCGCAGG608.353989E-539.530869
CTGGTCG853.6772872E-739.0657929
GTATCAA155050.035.0656051
TATCACG550.002594593734.5037082
TGGGACG1107.09133E-834.499666
ACGAACC300.003964684431.64509874-75
TGTCGCG404.3101635E-429.90057664-65
TAGATAG1452.4296241E-829.4436765
TCACGCA650.00588123329.1920244
GTACATG109650.028.6862221
TACATGG107750.028.3554842
CATATAG856.4556894E-427.9041373
TAGCGCG1205.435315E-627.6716044
ATGGGAT25950.027.237455
ACATGGG110200.027.076133
TATCAAC204350.026.605932
GCTCGCA909.015192E-426.3539077
CATGGGG20950.026.0394214
ATCAACG210250.025.8562853
Produced by FastQC (version 0.11.5)