##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939730_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 852863 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91489254428906 31.0 31.0 33.0 28.0 34.0 2 31.04824455979448 31.0 31.0 34.0 28.0 34.0 3 31.00888067602886 31.0 31.0 34.0 27.0 34.0 4 34.917504921657994 35.0 35.0 37.0 32.0 37.0 5 34.69851664335304 35.0 35.0 37.0 32.0 37.0 6 34.81760611024279 35.0 35.0 37.0 32.0 37.0 7 34.67027764130933 35.0 35.0 37.0 31.0 37.0 8 34.68939091038068 35.0 35.0 37.0 31.0 37.0 9 36.05098708702336 38.0 35.0 39.0 31.0 39.0 10-11 35.942348888391216 37.0 35.0 39.0 30.0 39.0 12-13 35.81361191656808 37.0 35.0 39.0 30.0 39.0 14-15 36.366432240582604 38.0 34.5 40.0 30.0 41.0 16-17 36.39965856180887 38.0 34.5 40.0 30.5 41.0 18-19 36.63774134884501 38.0 35.0 40.0 31.0 41.0 20-21 36.67438381076445 38.0 35.0 40.0 30.5 41.0 22-23 36.44428823855648 38.0 35.0 40.0 30.0 41.0 24-25 36.16820286493845 38.0 35.0 40.0 30.0 41.0 26-27 35.67518933287057 38.0 34.0 40.0 28.5 41.0 28-29 35.243297575343284 37.5 33.5 40.0 27.0 41.0 30-31 34.57648532062008 36.5 33.0 39.0 27.0 40.5 32-33 33.89691837962252 35.0 33.0 39.0 25.0 40.0 34-35 33.40491673340267 35.0 33.0 39.0 24.0 40.0 36-37 32.88024043720972 35.0 32.5 39.0 21.0 40.0 38-39 32.488850495331604 35.0 31.0 39.0 20.0 40.0 40-41 32.14017608924294 35.0 31.0 38.5 18.0 40.0 42-43 31.585708959117703 35.0 31.0 38.0 15.0 40.0 44-45 30.93353211477107 35.0 30.0 38.0 8.0 40.0 46-47 30.173056516697287 35.0 28.0 38.0 2.0 40.0 48-49 29.55017511605029 35.0 26.5 38.0 2.0 40.0 50-51 29.226065030374166 34.5 26.5 38.0 2.0 40.0 52-53 28.914852678566195 34.0 26.0 38.0 2.0 40.0 54-55 28.5767907624085 34.0 25.0 37.0 2.0 40.0 56-57 28.120811314361156 33.5 23.5 37.0 2.0 40.0 58-59 27.69356156850514 33.0 23.0 36.5 2.0 39.5 60-61 27.29755306538096 33.0 22.5 36.0 2.0 39.0 62-63 26.912847080949696 33.0 22.0 36.0 2.0 39.0 64-65 26.401263743414827 33.0 21.0 35.0 2.0 39.0 66-67 25.472000192293486 32.0 16.0 35.0 2.0 38.0 68-69 23.7182343471343 30.0 7.5 35.0 2.0 37.0 70-71 23.08310830696138 29.0 7.5 35.0 2.0 36.5 72-73 23.50387870033053 30.0 7.5 34.5 2.0 36.0 74-75 23.53528526856013 30.0 7.0 34.0 2.0 36.0 76-77 23.258404339266683 29.5 6.0 33.5 2.0 35.0 78-79 23.743565496451367 31.0 2.0 34.5 2.0 35.0 80-81 23.539070167189806 31.0 2.0 34.5 2.0 35.0 82-83 23.05108323376674 30.5 2.0 34.0 2.0 35.0 84-85 22.654901783756593 30.0 2.0 34.0 2.0 35.0 86-87 22.198793944631202 30.0 2.0 34.0 2.0 35.0 88-89 21.6642543995929 29.0 2.0 34.0 2.0 35.0 90-91 21.07085076970158 29.0 2.0 34.0 2.0 35.0 92-93 20.48711633638697 28.5 2.0 33.5 2.0 35.0 94-95 19.63894318313727 27.0 2.0 33.0 2.0 35.0 96-97 18.996354045139725 25.5 2.0 33.0 2.0 35.0 98-99 18.305831065481797 24.5 2.0 33.0 2.0 35.0 100-101 17.23009205464418 21.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.12726410090018803 1101 2 0.22025965491658184 1101 3 0.30488456208639647 1101 4 0.20257177201856535 1101 5 0.46383885368411626 1101 6 0.17586624875281842 1101 7 0.16253225485431955 1101 8 0.10003658820080119 1101 9 0.5504214327518611 1101 10-11 0.3696717070951294 1101 12-13 0.1868974072639915 1101 14-15 0.3889441862118943 1101 16-17 0.6451657690554882 1101 18-19 0.4865492471756738 1101 20-21 0.3280750648453221 1101 22-23 0.2764119682204793 1101 24-25 0.17363563414428285 1101 26-27 0.13892325403458727 1101 28-29 0.24104947320923742 1101 30-31 0.2602840191539215 1101 32-33 0.39168897247188994 1101 34-35 0.3939028088645884 1101 36-37 0.8769685256886461 1101 38-39 0.8833556017848423 1101 40-41 1.017992218914948 1101 42-43 0.595140060996755 1101 44-45 0.07351919614546176 1101 46-47 0.14957693694984187 1101 48-49 0.47049447299420777 1101 50-51 0.35360471785650205 1101 52-53 0.09642228088147675 1101 54-55 -0.009110612344535696 1101 56-57 0.011492639036529084 1101 58-59 -0.09074927139337419 1101 60-61 -0.04108470515689078 1101 62-63 0.08190812177289786 1101 64-65 -0.4161813515088255 1101 66-67 -0.6616424071161155 1101 68-69 -7.809915035967332 1101 70-71 -6.215293254684319 1101 72-73 -1.9855418526704867 1101 74-75 -1.033122999807592 1101 76-77 -0.6529372611627622 1101 78-79 -0.7488282744330199 1101 80-81 -0.5768564780617425 1101 82-83 -0.9030706961795154 1101 84-85 -0.779464830151607 1101 86-87 -0.8873411743743347 1101 88-89 -0.6504803229888374 1101 90-91 -0.8181170182744744 1101 92-93 -1.117064178181586 1101 94-95 -1.0872896165681247 1101 96-97 -1.58489142468407 1101 98-99 -1.0774237944827476 1101 100-101 -0.7817273046663047 1102 1 0.19609439163717113 1102 2 0.19176663499055735 1102 3 0.33722756013754207 1102 4 0.284976417446714 1102 5 0.28513702812630726 1102 6 0.2965718129257553 1102 7 0.2952968414191659 1102 8 0.284736106953531 1102 9 0.47831786515850183 1102 10-11 0.3267014966985968 1102 12-13 0.2582154679568376 1102 14-15 0.4617736019538583 1102 16-17 0.2946149199125827 1102 18-19 0.3460648181099302 1102 20-21 0.3028970829020352 1102 22-23 0.3274719053799302 1102 24-25 0.12889355881517162 1102 26-27 0.45164249994795824 1102 28-29 0.43707222307997284 1102 30-31 0.18209923611291856 1102 32-33 0.2654291784934415 1102 34-35 0.3958635931783121 1102 36-37 0.4170114799900446 1102 38-39 0.6603153917860354 1102 40-41 0.688135002194592 1102 42-43 0.9698308297992071 1102 44-45 0.8718925008425948 1102 46-47 0.7732535069693256 1102 48-49 0.4997252728192052 1102 50-51 0.19241273599276454 1102 52-53 0.8084813032733962 1102 54-55 0.40323676068175374 1102 56-57 0.34667340505490785 1102 58-59 0.08871220893912479 1102 60-61 0.03841853630196823 1102 62-63 0.4570642288404727 1102 64-65 0.2584915196680413 1102 66-67 0.3090512253064297 1102 68-69 -3.9195300381548215 1102 70-71 -0.1811664599501981 1102 72-73 0.2958883664759959 1102 74-75 0.1386444901498507 1102 76-77 0.013373441617339665 1102 78-79 -0.5413749076907841 1102 80-81 -0.6435052471028229 1102 82-83 -0.554947958240529 1102 84-85 -0.5073672284334343 1102 86-87 -0.3770878235410997 1102 88-89 -0.25531864777108026 1102 90-91 -0.33289846837987014 1102 92-93 -0.6908938723310527 1102 94-95 -0.39514667419847527 1102 96-97 -1.1479586799837591 1102 98-99 -0.8173195905293618 1102 100-101 -0.7167927304309316 1103 1 0.048185088885357885 1103 2 0.14065517477533618 1103 3 0.059828560320823954 1103 4 0.18989616713906798 1103 5 0.18283032270908706 1103 6 0.2545117495633562 1103 7 0.1384461096096743 1103 8 0.29995093689619523 1103 9 0.4896654943051999 1103 10-11 0.45652633371061313 1103 12-13 0.5333339451646992 1103 14-15 0.48935326314830974 1103 16-17 0.44365793826673183 1103 18-19 0.34325540277204425 1103 20-21 0.2578574159473561 1103 22-23 0.48641333443760715 1103 24-25 0.46589772188169576 1103 26-27 0.4021059356560741 1103 28-29 0.43597647254977545 1103 30-31 0.47128311797608546 1103 32-33 0.4525066534234057 1103 34-35 0.42076868049797866 1103 36-37 0.7432217803068539 1103 38-39 1.1614517256692096 1103 40-41 0.8332034702384803 1103 42-43 0.4113096348158294 1103 44-45 0.8677228886099861 1103 46-47 0.580892341310701 1103 48-49 0.5003876978709165 1103 50-51 0.2825693091868047 1103 52-53 0.53203535768127 1103 54-55 0.5416351582938823 1103 56-57 0.8970962574100518 1103 58-59 0.5193290759042704 1103 60-61 0.30680672261883046 1103 62-63 0.0643469769880376 1103 64-65 0.09734157072451666 1103 66-67 0.2328291427258513 1103 68-69 -3.6544539770965123 1103 70-71 0.4227452516199861 1103 72-73 0.3349923907736425 1103 74-75 0.34987429665889636 1103 76-77 0.14132751697145807 1103 78-79 -0.02167234439384913 1103 80-81 0.10088508620432535 1103 82-83 -0.12724131042548237 1103 84-85 -0.3178194055339496 1103 86-87 -0.5644009437463744 1103 88-89 -0.562516301791451 1103 90-91 -0.5039402823248338 1103 92-93 -0.6689055498635845 1103 94-95 -0.5066867469866843 1103 96-97 -0.9283058640227146 1103 98-99 -0.7814111779930322 1103 100-101 -0.5983849070077909 1104 1 0.13481901596198043 1104 2 0.27688298550127044 1104 3 0.4586672857090548 1104 4 0.1478031332647518 1104 5 0.3314749950003346 1104 6 0.3374954491032085 1104 7 -0.04657654260151389 1104 8 0.12911825886198613 1104 9 0.32338608828523974 1104 10-11 0.39067891778760355 1104 12-13 0.48698949964923344 1104 14-15 0.4595489904288783 1104 16-17 0.6398367410732746 1104 18-19 0.38322146250488487 1104 20-21 0.5557847259401996 1104 22-23 0.4896957489147269 1104 24-25 0.41075747095709403 1104 26-27 0.5841860833900867 1104 28-29 0.41411315695602013 1104 30-31 0.0742618964308619 1104 32-33 0.190417018649093 1104 34-35 0.2895532739503963 1104 36-37 0.46344160568443726 1104 38-39 0.38135320250205496 1104 40-41 0.454610951302449 1104 42-43 0.584713199304872 1104 44-45 0.6541160883806754 1104 46-47 0.7377570013246029 1104 48-49 0.7291636160084138 1104 50-51 0.6114563322347344 1104 52-53 0.5077926720878736 1104 54-55 0.5157626803499831 1104 56-57 0.6773520523682244 1104 58-59 0.6845202625960347 1104 60-61 0.5556867952682509 1104 62-63 0.8166743458309753 1104 64-65 0.7966721509559633 1104 66-67 0.7467121832461672 1104 68-69 -3.5416583949118525 1104 70-71 0.3353376487972106 1104 72-73 0.7370539195569172 1104 74-75 0.5006814560766877 1104 76-77 0.23548131863651278 1104 78-79 0.057785423056980534 1104 80-81 0.032454100617520965 1104 82-83 0.08518848801296386 1104 84-85 0.05608539937292889 1104 86-87 0.008921955533725878 1104 88-89 0.08207735356010204 1104 90-91 0.1747724734320748 1104 92-93 -3.926307469477308E-4 1104 94-95 0.2713203150240595 1104 96-97 -0.012108898860951456 1104 98-99 0.33701955392958993 1104 100-101 0.3022687855816635 1105 1 0.06138174795901108 1105 2 -0.03271612363013077 1105 3 -0.1845348919697365 1105 4 0.12103005729345995 1105 5 0.06142055303052274 1105 6 0.007146525569432072 1105 7 -0.027959863561676457 1105 8 0.0584525820493198 1105 9 0.043720164009073414 1105 10-11 0.08719958098978253 1105 12-13 -0.09253165847994893 1105 14-15 -0.0033030263986333352 1105 16-17 -0.08124714905293473 1105 18-19 -0.24226851522340098 1105 20-21 -0.23687303204049215 1105 22-23 -0.16676181109516364 1105 24-25 -0.09934398907755337 1105 26-27 0.078060124469026 1105 28-29 0.09653582461253762 1105 30-31 -0.019414173848772975 1105 32-33 -0.11809572572111193 1105 34-35 -0.43969196237724617 1105 36-37 -0.5455747269065085 1105 38-39 -0.09441640898025128 1105 40-41 -0.334489518878204 1105 42-43 -0.4217592085149775 1105 44-45 -0.27822244168613963 1105 46-47 -0.07477331295404355 1105 48-49 0.0696486444667137 1105 50-51 -0.11040335596125672 1105 52-53 -0.6197945587955687 1105 54-55 -0.41003910138721267 1105 56-57 -0.36275188230141353 1105 58-59 -0.10525330830225599 1105 60-61 -0.04125579319995154 1105 62-63 0.2005125047025409 1105 64-65 -0.006604265772573115 1105 66-67 0.04895543986581785 1105 68-69 1.231734329661272 1105 70-71 1.5359216626551628 1105 72-73 0.9486661442537745 1105 74-75 0.2466713100732214 1105 76-77 0.01661098951005613 1105 78-79 0.23542585859273757 1105 80-81 0.0872227222458335 1105 82-83 0.07085663946062226 1105 84-85 0.1388396681182904 1105 86-87 -0.27331387718095 1105 88-89 0.1336277123821752 1105 90-91 0.2913735622714704 1105 92-93 0.15954674069577024 1105 94-95 0.43027478174021994 1105 96-97 -0.2716176838151725 1105 98-99 -0.316649092445072 1105 100-101 -0.21792299863016495 1106 1 0.35120630936252084 1106 2 0.4241030983378309 1106 3 0.40327085427994547 1106 4 0.36536517043864336 1106 5 0.3001111200277222 1106 6 0.3051938177128335 1106 7 0.17989927704346087 1106 8 0.4428360043732482 1106 9 0.5137659305994617 1106 10-11 0.336991088744675 1106 12-13 0.5073882499868674 1106 14-15 0.6687043471075924 1106 16-17 0.541674284966895 1106 18-19 0.576919127797197 1106 20-21 0.45933596872228577 1106 22-23 0.2359676575038634 1106 24-25 0.398853315778787 1106 26-27 0.4899759647944819 1106 28-29 0.5443593689807074 1106 30-31 0.5390234056071108 1106 32-33 0.529018436496024 1106 34-35 0.6183273612942557 1106 36-37 0.8620415965107355 1106 38-39 0.8656916510248323 1106 40-41 0.8765618414065628 1106 42-43 0.4902074835775778 1106 44-45 0.6807249267349142 1106 46-47 0.6048363997334718 1106 48-49 0.6493715023360274 1106 50-51 0.5474799392332521 1106 52-53 0.7983442665286091 1106 54-55 1.141230921496085 1106 56-57 0.9160505585834038 1106 58-59 0.912271481934205 1106 60-61 0.9755723278272548 1106 62-63 1.115356135518713 1106 64-65 1.1466553324984687 1106 66-67 1.2069785774198465 1106 68-69 2.4753422625369375 1106 70-71 3.0169081869368846 1106 72-73 1.8138136143783612 1106 74-75 1.2304406973094295 1106 76-77 0.8931467128764439 1106 78-79 0.9822514370315893 1106 80-81 1.0873727349587767 1106 82-83 0.7085111017031842 1106 84-85 0.815529218080151 1106 86-87 0.9055432293535013 1106 88-89 0.9462147960333311 1106 90-91 0.7104798424647072 1106 92-93 0.8590522072607278 1106 94-95 1.188130108462822 1106 96-97 0.7815923339829425 1106 98-99 0.9212545434562784 1106 100-101 1.0655082243548328 1107 1 -0.06950785547078553 1107 2 -0.1541845051949764 1107 3 -0.03804686052993844 1107 4 -0.02226754899449901 1107 5 -0.08316322689088196 1107 6 0.12474566812034027 1107 7 0.1144231303647274 1107 8 -0.0605756423165289 1107 9 0.13020462274433697 1107 10-11 -0.08043528860650184 1107 12-13 -0.09174387712946697 1107 14-15 0.10968911365137757 1107 16-17 0.014894330046743676 1107 18-19 0.10833705819567996 1107 20-21 0.01520628200023566 1107 22-23 -0.16057924589330952 1107 24-25 0.04409295268736457 1107 26-27 -0.08514280514868489 1107 28-29 -0.05811940939960891 1107 30-31 -0.05960531354151044 1107 32-33 0.3085030595593423 1107 34-35 0.36392361461454215 1107 36-37 0.20110801693057567 1107 38-39 0.4074824834813455 1107 40-41 0.07858479337617297 1107 42-43 -0.004451241383858218 1107 44-45 -0.003259955083780852 1107 46-47 0.050616111840525946 1107 48-49 0.49833746190151373 1107 50-51 0.4766330856250214 1107 52-53 0.1958967595324026 1107 54-55 0.4896339961476208 1107 56-57 0.23267148318625885 1107 58-59 0.6058131440021377 1107 60-61 0.7495820060140552 1107 62-63 0.39218453042601453 1107 64-65 0.16606811722135717 1107 66-67 0.6380104595156944 1107 68-69 1.9215628398434816 1107 70-71 1.93496060513462 1107 72-73 1.0225764693698878 1107 74-75 0.639577705214343 1107 76-77 0.5589457519252079 1107 78-79 0.6404079950700527 1107 80-81 0.7986446586516927 1107 82-83 0.6934665001251439 1107 84-85 0.41447739587820465 1107 86-87 0.3948122392892195 1107 88-89 0.398709884586534 1107 90-91 0.24145752082809935 1107 92-93 0.08004156027418574 1107 94-95 0.4514198335444348 1107 96-97 0.5543290828872216 1107 98-99 0.6893441194163081 1107 100-101 0.6275414529596901 1108 1 -0.10800726982907705 1108 2 -0.2805351491521755 1108 3 -0.2862569852927592 1108 4 -0.11237803757634879 1108 5 -0.24058769651833245 1108 6 -0.2127916028141712 1108 7 -0.09018466376791423 1108 8 -0.12287250173515929 1108 9 -0.21769514921598443 1108 10-11 -0.32409456698649564 1108 12-13 -0.32189360743586093 1108 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0.03175971829434587 2316 98-99 0.1412652262949976 2316 100-101 -0.027857256685358323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 25.0 4 102.0 5 338.0 6 791.0 7 1390.0 8 2435.0 9 3934.0 10 6104.0 11 8541.0 12 10854.0 13 13103.0 14 15364.0 15 18201.0 16 21084.0 17 23198.0 18 18573.0 19 14199.0 20 13748.0 21 14030.0 22 15012.0 23 16105.0 24 17498.0 25 19532.0 26 22650.0 27 26340.0 28 30094.0 29 35049.0 30 39441.0 31 44944.0 32 51756.0 33 61403.0 34 75791.0 35 89712.0 36 63048.0 37 44481.0 38 13583.0 39 405.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.534158810056304 21.84397645538356 15.909034935773017 15.712829798787117 2 16.586158167244243 26.905788050967217 36.670478066857825 19.83757571493072 3 19.89534075226619 33.155618194246905 30.729202697267908 16.219838356218993 4 11.877757623440106 21.012870765879164 35.23309136402916 31.87628024665157 5 10.586694463237354 43.59645101264798 30.178821217475726 15.638033306638931 6 25.26818492536316 39.26937855200659 17.38297944687482 18.079457075755425 7 22.85888823879099 40.983604635211044 19.78641352714328 16.37109359885468 8 27.592356568405474 32.82954003163462 19.783716728243576 19.794386671716325 9 28.577626183806775 17.365391627963696 20.40374597092382 33.65323621730571 10-11 23.959064937744984 31.21925795819493 23.95167805380231 20.86999905025778 12-13 26.600696712133132 30.334121658460973 27.045551278458557 16.019630350947338 14-15 22.574844963376297 29.401556873730012 30.06901460140726 17.95458356148643 16-17 16.624993038110116 33.24371304702134 30.100808165490715 20.030485749377828 18-19 16.051757433491662 28.21690001793958 38.89077143691308 16.840571111655684 20-21 17.711461278071624 27.21058364590796 39.69517964784497 15.382775428175451 22-23 16.938535262990655 28.351857215050952 39.555884122068846 15.153723399889548 24-25 16.272914023605225 29.146040039302957 39.72952247843145 14.85152345866037 26-27 15.830823644870826 29.35646643681301 39.89041597023667 14.922293948079496 28-29 15.31935999024461 29.664588174874716 40.10355719917173 14.912494635708942 30-31 15.147087964336341 30.151948099153785 39.730107157471885 14.970856779037986 32-33 15.029431077341588 30.62620186670419 39.287967262323534 15.056399793630693 34-35 15.468387232944819 30.509720691353394 39.055896906732954 14.965995168968831 36-37 15.55069700794443 30.660017263100332 38.535558642024206 15.253727086931034 38-39 15.340970765552346 30.84077273994103 38.669678611767125 15.1485778827395 40-41 14.953450353889973 30.915114522808917 38.800153972357734 15.331281150943374 42-43 15.043076283926753 31.227988024781332 37.947324998562365 15.78161069272955 44-45 15.107031414110239 31.31177879509419 37.27663404656315 16.304555744232417 46-47 15.275374614736048 31.52936220297345 36.37677919630405 16.818483985986454 48-49 15.443668384637771 31.678612667924632 35.629300795148374 17.24841815228922 50-51 15.33407066016176 31.967401157622678 35.133642932370314 17.56488524984525 52-53 15.683508102955196 32.10313118721126 34.58807655642737 17.625284153406174 54-55 15.20289254889447 31.89001134447916 34.42750267774288 18.47959342888349 56-57 15.662121396937398 31.933549767302203 34.00789108242005 18.39643775334034 58-59 15.819472727240743 31.42188211854285 34.1595069578122 18.59913819640421 60-61 15.964874941588203 31.70413523276478 34.219316436859856 18.111673388787164 62-63 16.229123661325943 31.58834002951283 34.169745072254834 18.012791236906395 64-65 16.01932640184423 31.465966560470342 34.30131457524011 18.213392462445313 66-67 16.238617119476498 31.238828511634793 34.135253400477744 18.387300968410965 68-69 16.8329569085912 30.555817234208416 34.076526875313526 18.534698981886866 70-71 16.722425549675837 30.598528389346335 33.80989860968762 18.869147451290207 72-73 15.955046676107813 30.519883675374178 33.972206379293226 19.552863269224783 74-75 15.281833071689906 30.816004954121016 33.90200950360857 20.00015247058051 76-77 15.068522070798027 30.54139150618367 34.30138217012849 20.08870425288981 78-79 14.806144285169875 30.43015859056665 34.395567672148445 20.36812945211503 80-81 14.700583513757687 30.640012522710602 34.03761855096526 20.621785412566446 82-83 14.605461020973486 30.355244837239297 33.697342772135016 21.3419513696522 84-85 14.511920221138935 29.921938893660432 33.726091404484336 21.8400494807163 86-87 14.756639502843408 29.760802016767308 33.67725860350589 21.805299876883392 88-89 14.590830158724573 29.6014825393587 33.6055347823385 22.20215251957822 90-91 14.496944981526841 29.547205341093896 33.58415634940828 22.37169332797098 92-93 14.448337946322049 29.512203558557793 33.396846204851876 22.642612290268286 94-95 14.417113479247126 28.932918850855543 33.55225096139516 23.097716708502173 96-97 14.348077711278199 28.752914935198582 33.386496513218574 23.51251084030465 98-99 14.393798576885978 28.66745044461319 33.07288841718878 23.865862561312056 100-101 14.048992706207933 28.369450503060577 33.260160885574244 24.32139590515725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16873.0 1 11004.0 2 6722.5 3 8726.0 4 7607.5 5 6627.0 6 10708.0 7 19832.0 8 26139.5 9 24698.5 10 17598.0 11 11139.0 12 8867.5 13 7621.5 14 6682.0 15 5725.5 16 4878.5 17 4446.0 18 4248.5 19 4155.0 20 4129.0 21 4122.0 22 4290.0 23 4547.0 24 4826.5 25 5245.5 26 5824.5 27 6416.5 28 7115.5 29 8190.5 30 9156.0 31 10295.5 32 11950.5 33 13470.0 34 14820.5 35 16004.5 36 17639.5 37 19222.5 38 19809.5 39 20043.0 40 20348.5 41 20892.0 42 21593.5 43 22081.5 44 22137.5 45 21983.0 46 21877.5 47 22894.0 48 26103.0 49 26839.5 50 24475.5 51 21537.0 52 18513.0 53 17487.0 54 16938.0 55 15618.5 56 14349.0 57 13065.5 58 11840.0 59 10769.5 60 9701.0 61 8319.0 62 6599.0 63 5037.0 64 3949.5 65 3302.5 66 2775.0 67 2245.5 68 1561.0 69 1023.5 70 783.0 71 661.0 72 578.5 73 550.5 74 443.5 75 320.0 76 248.5 77 160.5 78 88.0 79 57.5 80 40.5 81 30.0 82 27.0 83 18.5 84 9.5 85 5.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021574391197648392 2 0.018525835919719815 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.448866934079682E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1725212607417604E-4 26-27 1.7587818911126406E-4 28-29 1.1725212607417604E-4 30-31 7.035127564450562E-4 32-33 0.002696798899706049 34-35 0.005041841421189569 36-37 0.08870123337511417 38-39 0.08213511431496032 40-41 0.08934612006852213 42-43 0.09028413707711555 44-45 0.08559405203414851 46-47 0.08061083667599603 48-49 0.08149022762155235 50-51 0.08037633242384767 52-53 0.06454729540383392 54-55 0.05463949075056603 56-57 0.029899292148914892 58-59 0.013659872687641508 60-61 0.009556048275045347 62-63 0.0015829037020013764 64-65 0.0046900850429670415 66-67 0.16004915209125029 68-69 0.42738399954037165 70-71 0.4129033619702109 72-73 0.19047607880749898 74-75 0.028081884194765164 76-77 0.0298406660858778 78-79 0.004455580790818689 80-81 0.0014656515759272006 82-83 5.862606303708802E-5 84-85 6.448866934079682E-4 86-87 0.0015242776389642887 88-89 2.3450425214835207E-4 90-91 4.6900850429670415E-4 92-93 0.002051912206298081 94-95 0.08395252226911004 96-97 0.016708427965570084 98-99 0.018232705604534376 100-101 0.00973192646415661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 852863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.81224835957232 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4238960940169 27.028986985403826 2 11.836142188120677 8.714300116966205 3 5.016141413821867 5.539663305970405 4 2.6755535137404234 3.939725617885555 5 1.6392536052306055 3.0172305420036687 6 1.0739456859889234 2.372061319038931 7 0.7615371521775096 1.9623726346699937 8 0.5462414711025556 1.608670135882034 9 0.42514377114961055 1.4085448282876178 >10 2.322474206136347 15.826038813615801 >50 0.17777040122813212 4.520706752698768 >100 0.09174219226109057 6.24987388777505 >500 0.004444258448632339 1.1751380242969878 >1k 0.0031744703204516704 2.1680922598231915 >5k 0.0012697881281806681 3.3550330824946815 >10k+ 0.0012697881281806681 11.113561693187362 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30077 3.5265921959329924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22514 2.6398143664339995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21447 2.5147063479128535 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21064 2.469798783626444 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8967 1.0513998145071364 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7145 0.8377664407999879 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6634 0.7778506043760839 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5964 0.699291679906386 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3727 0.4369986738784541 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3540 0.41507252630258323 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2390 0.28023258131728074 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1396 0.16368396799954976 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1370 0.16063541272162116 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1365 0.16004915209125029 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1351 0.15840762232621183 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1251 0.14668240971879423 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1117 0.13097062482485464 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1046 0.12264572387358813 RNA PCR Primer, Index 39 (95% over 23bp) GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 999 0.11713487394810188 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 985 0.11549334418306341 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 905 0.10611317409712931 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 888 0.10411988795386834 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 880 0.10318187094527492 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1725212607417604E-4 0.0 14-15 0.0 0.0 0.0 1.1725212607417604E-4 0.0 16-17 0.0 0.0 0.0 5.276345673337922E-4 0.0 18-19 0.0 5.862606303708802E-5 0.0 6.448866934079682E-4 0.0 20-21 0.0 1.1725212607417604E-4 0.0 0.0017587818911126408 0.0 22-23 0.0 1.1725212607417604E-4 0.0 0.00562810205156045 0.0 24-25 0.0 1.1725212607417604E-4 0.0 0.01389437693978986 0.0 26-27 0.0 1.1725212607417604E-4 0.0 0.025678215610244554 0.0 28-29 0.0 1.1725212607417604E-4 0.0 0.04948039720330229 0.0 30-31 0.0 1.1725212607417604E-4 0.0 0.11725212607417604 0.0 32-33 0.0 1.1725212607417604E-4 0.0 0.2060119855123273 0.0 34-35 0.0 1.1725212607417604E-4 0.0 0.32068456481287144 0.0 36-37 0.0 1.1725212607417604E-4 0.0 0.4906415215573896 0.0 38-39 0.0 1.1725212607417604E-4 0.0 0.7430267229320535 0.0 40-41 0.0 1.1725212607417604E-4 0.0 1.0269527462206707 0.0 42-43 0.0 1.1725212607417604E-4 0.0 1.2816243640537812 0.0 44-45 0.0 1.1725212607417604E-4 0.0 1.557166860328095 0.0 46-47 0.0 1.1725212607417604E-4 0.0 1.873571722539259 0.0 48-49 0.0 1.1725212607417604E-4 0.0 2.2087369249222912 0.0 50-51 0.0 1.1725212607417604E-4 0.0 2.5654178924399345 0.0 52-53 0.0 1.1725212607417604E-4 0.0 2.8934307151324425 0.0 54-55 0.0 1.1725212607417604E-4 0.0 3.2088975603350125 0.0 56-57 0.0 1.1725212607417604E-4 0.0 3.5563742359558335 0.0 58-59 0.0 1.1725212607417604E-4 0.0 3.9152829938688862 0.0 60-61 0.0 1.1725212607417604E-4 0.0 4.287382615965284 0.0 62-63 0.0 1.1725212607417604E-4 0.0 4.634742039460031 0.0 64-65 0.0 1.1725212607417604E-4 0.0 4.964513644043651 0.0 66-67 0.0 1.1725212607417604E-4 0.0 5.3199048381744785 0.0 68-69 0.0 1.1725212607417604E-4 0.0 5.705781585084591 0.0 70-71 0.0 1.1725212607417604E-4 0.0 6.166699692682178 0.0 72-73 0.0 1.1725212607417604E-4 0.0 6.64198118572385 0.0 74-75 0.0 1.1725212607417604E-4 0.0 7.108586021436034 0.0 76-77 0.0 1.1725212607417604E-4 0.0 7.576891012976293 0.0 78-79 0.0 1.1725212607417604E-4 0.0 8.099952747393193 0.0 80-81 0.0 1.1725212607417604E-4 0.0 8.635677711426101 0.0 82-83 0.0 1.1725212607417604E-4 0.0 9.180255210977613 0.0 84-85 0.0 2.3450425214835207E-4 0.0 9.715745670758375 0.0 86-87 0.0 2.3450425214835207E-4 0.0 10.238338396670978 0.0 88-89 0.0 2.3450425214835207E-4 0.0 10.762983034789878 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9775 0.0 41.49711 1 TCGCAGG 60 8.353989E-5 39.53086 9 CTGGTCG 85 3.6772872E-7 39.065792 9 GTATCAA 15505 0.0 35.065605 1 TATCACG 55 0.0025945937 34.503708 2 TGGGACG 110 7.09133E-8 34.49966 6 ACGAACC 30 0.0039646844 31.645098 74-75 TGTCGCG 40 4.3101635E-4 29.900576 64-65 TAGATAG 145 2.4296241E-8 29.443676 5 TCACGCA 65 0.005881233 29.192024 4 GTACATG 10965 0.0 28.686222 1 TACATGG 10775 0.0 28.355484 2 CATATAG 85 6.4556894E-4 27.904137 3 TAGCGCG 120 5.435315E-6 27.671604 4 ATGGGAT 2595 0.0 27.23745 5 ACATGGG 11020 0.0 27.07613 3 TATCAAC 20435 0.0 26.60593 2 GCTCGCA 90 9.015192E-4 26.353907 7 CATGGGG 2095 0.0 26.039421 4 ATCAACG 21025 0.0 25.856285 3 >>END_MODULE