FastQCFastQC Report
Fri 27 May 2016
SRR939729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744460
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87390.5009573163041858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.3444618965181202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43950.251940428556688No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.047.180771
GTATCAA72750.043.2886351
TATCAAC92600.035.187192
ATCAACG92600.035.0355453
TCAACGC94150.034.56054
CAACGCA96800.033.516375
AACGCAG98850.032.9652446
ACGCAGA113950.028.388757
CGCAGAG115900.027.868598
TACATGG85950.027.1492652
GTACATG86600.027.1097891
ACATGGG87300.025.9159973
TATCACG1109.577038E-525.8700562
GCAGAGT119350.025.7125769
TGGTATC21050.023.6578922
CATGGGG37700.023.0242754
GTGGTAT22850.022.8320731
GAGTACT69050.022.32362612-13
GGACCGA2555.002221E-1022.3211866
AGTACTT73100.021.89784812-13
Produced by FastQC (version 0.11.5)