##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939729_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1744460 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.428647833713583 31.0 30.0 33.0 23.0 34.0 2 30.336630819852562 31.0 30.0 34.0 26.0 34.0 3 30.661652316476157 31.0 31.0 34.0 27.0 34.0 4 34.47193859417814 35.0 35.0 37.0 32.0 37.0 5 34.45533058940876 37.0 35.0 37.0 30.0 37.0 6 34.52437201196932 37.0 35.0 37.0 30.0 37.0 7 34.45712713389817 37.0 35.0 37.0 30.0 37.0 8 34.3463043004712 37.0 35.0 37.0 30.0 37.0 9 35.70220870641918 38.0 35.0 39.0 30.0 39.0 10-11 35.556473063297524 38.0 35.0 39.0 30.0 39.0 12-13 35.44607500315284 38.0 35.0 39.0 30.0 39.0 14-15 36.20893944257821 38.0 35.5 40.0 29.5 41.0 16-17 36.050826043589424 38.0 35.0 40.0 29.0 41.0 18-19 35.91734404916134 38.0 35.0 40.0 27.0 41.0 20-21 36.139977987457435 39.0 35.0 40.0 29.0 41.0 22-23 36.05730942526627 39.0 35.0 40.0 28.0 41.0 24-25 35.86772067000676 38.5 35.0 40.0 27.0 41.0 26-27 35.778344014766745 38.5 34.5 40.0 27.0 41.0 28-29 35.83428367517742 38.5 35.0 40.0 27.0 41.0 30-31 35.61254256331472 38.0 35.0 40.0 27.0 41.0 32-33 35.289677894592025 38.0 34.0 40.0 26.5 41.0 34-35 35.11653950219552 38.0 34.0 40.0 25.5 41.0 36-37 34.90635956112493 38.0 34.0 40.0 25.0 41.0 38-39 34.69772135789872 38.0 34.0 40.0 24.5 41.0 40-41 34.46937447691549 38.0 33.0 40.0 24.0 41.0 42-43 34.09148074475769 38.0 33.0 40.0 23.0 41.0 44-45 33.74236841200142 38.0 33.0 40.0 22.0 41.0 46-47 33.325974513603065 37.5 32.5 40.0 19.0 41.0 48-49 32.9556553317359 37.0 31.5 40.0 16.5 41.0 50-51 32.342514015798585 36.5 30.5 39.5 15.0 40.5 52-53 32.50610618758814 37.0 31.0 39.0 14.5 40.0 54-55 32.61349873313232 37.0 31.0 40.0 13.5 41.0 56-57 32.3327906630132 37.0 31.0 39.5 10.5 41.0 58-59 31.958925971360763 36.0 31.0 39.0 8.0 40.0 60-61 31.569556768283594 36.0 30.0 39.0 7.5 40.0 62-63 31.14326496451624 35.0 29.5 39.0 4.5 40.0 64-65 30.61950947571168 35.0 29.0 38.0 2.0 40.0 66-67 30.03330314251975 34.5 28.0 38.0 2.0 40.0 68-69 29.485117170929687 34.0 28.0 37.5 2.0 39.5 70-71 28.915148240716327 34.0 26.5 37.0 2.0 39.0 72-73 28.255755649312682 33.5 26.0 36.0 2.0 39.0 74-75 27.91328892608601 33.0 26.0 36.0 2.0 38.5 76-77 27.362505589122136 33.0 25.5 35.0 2.0 38.0 78-79 26.698803641241415 32.0 24.5 35.0 2.0 37.0 80-81 25.973410109718767 32.0 21.5 35.0 2.0 36.5 82-83 25.231361854098118 31.0 19.0 34.5 2.0 36.0 84-85 24.45804460979329 31.0 15.5 34.0 2.0 35.5 86-87 23.55374843791202 30.5 7.0 34.0 2.0 35.0 88-89 22.574590704286713 29.5 2.0 34.0 2.0 35.0 90-91 21.139568118500854 28.0 2.0 33.5 2.0 35.0 92-93 19.56477247973585 25.5 2.0 33.0 2.0 34.5 94-95 19.04539026403586 25.0 2.0 32.0 2.0 34.0 96-97 18.730276704538937 25.0 2.0 32.0 2.0 34.0 98-99 17.800705949118928 24.5 2.0 32.0 2.0 34.0 100-101 16.02983702693097 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3878643375405773 1101 2 0.772613111750065 1101 3 0.5746917739535036 1101 4 0.5889982990496136 1101 5 0.5726534271873334 1101 6 0.5821052183680848 1101 7 0.6164476958148697 1101 8 0.8509147607656402 1101 9 0.7245994379127438 1101 10-11 0.898460269978905 1101 12-13 0.7731598515952811 1101 14-15 0.8278679217717055 1101 16-17 0.9597947332263317 1101 18-19 0.7353881334213384 1101 20-21 0.7393893256797099 1101 22-23 0.9265920807407682 1101 24-25 1.1039223284005004 1101 26-27 0.9032486882604545 1101 28-29 0.8590980601162599 1101 30-31 1.0588886769515042 1101 32-33 1.1655600382549025 1101 34-35 0.86206688058639 1101 36-37 0.8120995714247456 1101 38-39 0.8087378511853061 1101 40-41 0.6741352544477479 1101 42-43 0.8227029996281345 1101 44-45 0.6377029225713571 1101 46-47 0.4870281168856394 1101 48-49 0.5629208040659748 1101 50-51 0.47470180025317177 1101 52-53 0.47856047209985775 1101 54-55 0.5741368550648573 1101 56-57 0.4441195356407306 1101 58-59 0.39115980598748123 1101 60-61 0.506598154382246 1101 62-63 0.26815720858332526 1101 64-65 0.07330595378236282 1101 66-67 0.12723518843659676 1101 68-69 0.24573374430274697 1101 70-71 0.3733185053036223 1101 72-73 0.09927097954938802 1101 74-75 0.2841191440928874 1101 76-77 0.3098223380035776 1101 78-79 -0.06592282029858154 1101 80-81 -0.3712970092305632 1101 82-83 -0.4302871621590505 1101 84-85 -0.7740800079713885 1101 86-87 -1.027873548910037 1101 88-89 -4.97232069612863 1101 90-91 -8.271299362484072 1101 92-93 -10.861357226915805 1101 94-95 -8.921482347454354 1101 96-97 -5.319472113501195 1101 98-99 -3.776223922636291 1101 100-101 -2.73787172248951 1102 1 1.6508397436009403 1102 2 1.0755736973582053 1102 3 1.0057716642020438 1102 4 0.7945778321386143 1102 5 0.7815108170520162 1102 6 0.9245152442037323 1102 7 0.677271632847976 1102 8 1.077010940159873 1102 9 1.0974331150538674 1102 10-11 1.141741831867641 1102 12-13 1.146914800090876 1102 14-15 1.3754894728455156 1102 16-17 1.2936558782731638 1102 18-19 1.3901250036355677 1102 20-21 1.3634392645954136 1102 22-23 1.2360307728024864 1102 24-25 1.440967450669234 1102 26-27 1.5311318336677857 1102 28-29 1.1358896157964296 1102 30-31 1.2070886095251225 1102 32-33 1.4157135834806454 1102 34-35 1.3972746339526978 1102 36-37 1.38111397797244 1102 38-39 1.4500390969043977 1102 40-41 1.1321952939689481 1102 42-43 1.096580951201311 1102 44-45 1.1694008323535243 1102 46-47 1.184914533473865 1102 48-49 1.260930307180267 1102 50-51 1.4609845370966852 1102 52-53 1.4293225237311091 1102 54-55 1.3457658697718173 1102 56-57 1.364640688164549 1102 58-59 1.2792541261493007 1102 60-61 1.1034380434291364 1102 62-63 1.1471126671160974 1102 64-65 0.9866784912173578 1102 66-67 1.1657833849477832 1102 68-69 0.6759536978386294 1102 70-71 0.8476101110528909 1102 72-73 0.976246622603469 1102 74-75 0.8458476627820097 1102 76-77 0.7458661618137725 1102 78-79 0.7911136400166541 1102 80-81 0.723938582552762 1102 82-83 0.7418691635704597 1102 84-85 0.718853990947121 1102 86-87 0.8847387727605316 1102 88-89 0.6250257252662301 1102 90-91 -5.898572916655496 1102 92-93 -11.385941569975895 1102 94-95 -9.141947839833838 1102 96-97 -5.200974420017522 1102 98-99 -3.351249541323412 1102 100-101 -2.3867133739685524 1103 1 1.9739602289084637 1103 2 1.239040630523224 1103 3 0.9753873455261228 1103 4 0.8310372612710921 1103 5 0.9035302433792438 1103 6 0.7510195572691671 1103 7 0.779452711464991 1103 8 0.889769548757954 1103 9 1.0060785319835261 1103 10-11 0.9072495813591956 1103 12-13 0.9612688922432042 1103 14-15 1.055402159569688 1103 16-17 1.058703392124066 1103 18-19 0.819888661676103 1103 20-21 0.8544317956284431 1103 22-23 0.8705949077348052 1103 24-25 0.8873872557046525 1103 26-27 0.9111725097320189 1103 28-29 0.836828496503351 1103 30-31 0.8723921020561605 1103 32-33 0.9040893461568587 1103 34-35 0.7007583985925763 1103 36-37 0.7593926090124867 1103 38-39 0.7785114652331728 1103 40-41 0.5641987654606453 1103 42-43 0.7494668478280033 1103 44-45 0.7780076771533047 1103 46-47 0.9378074432150854 1103 48-49 0.8768351742817515 1103 50-51 0.6193932614111546 1103 52-53 0.730360464680679 1103 54-55 0.6959394443562914 1103 56-57 0.5568789236649323 1103 58-59 0.6524613987490646 1103 60-61 0.7157311565506674 1103 62-63 0.46002907079097 1103 64-65 0.3630065997803733 1103 66-67 0.4880885956918739 1103 68-69 0.4531952645684143 1103 70-71 0.28945824205786863 1103 72-73 0.5822191662601064 1103 74-75 0.29668572996764553 1103 76-77 -0.06617798574215783 1103 78-79 -0.13021236461200303 1103 80-81 -0.33422643603199376 1103 82-83 -0.4994126584366967 1103 84-85 -0.6476645236752212 1103 86-87 -0.529021834575186 1103 88-89 -1.1546528987606983 1103 90-91 -6.937841974794214 1103 92-93 -10.819409029124502 1103 94-95 -9.414858808344954 1103 96-97 -5.920861859529408 1103 98-99 -4.17648711057792 1103 100-101 -3.519905568372087 1104 1 1.936586510476154 1104 2 1.1191109457541337 1104 3 1.0155010211750017 1104 4 0.7471111961208337 1104 5 0.7034152791306738 1104 6 0.7548211667686502 1104 7 0.7627920310170424 1104 8 0.8922522668570565 1104 9 0.9555046002591112 1104 10-11 0.8440453291725376 1104 12-13 0.8039079107533595 1104 14-15 0.8778439405875602 1104 16-17 0.8050896633444893 1104 18-19 0.836264576016923 1104 20-21 0.7492492448068901 1104 22-23 0.8432252666627136 1104 24-25 0.8153952825267581 1104 26-27 0.6916603937695882 1104 28-29 0.5642657409771559 1104 30-31 0.731304980696514 1104 32-33 0.7676477949713174 1104 34-35 0.8987179634882381 1104 36-37 0.8752515448503217 1104 38-39 0.7812082687847095 1104 40-41 0.5851355053130334 1104 42-43 0.8211007297297641 1104 44-45 0.7220794454246118 1104 46-47 0.712566828476092 1104 48-49 0.7268413287877351 1104 50-51 0.6875098190997875 1104 52-53 0.6275975242308149 1104 54-55 0.6770029189499596 1104 56-57 0.6773401558066467 1104 58-59 0.46384081586926484 1104 60-61 0.4955718558179427 1104 62-63 0.5642532255921822 1104 64-65 0.4964299187227361 1104 66-67 0.5470522490066294 1104 68-69 0.6064602540360369 1104 70-71 0.39305083876415026 1104 72-73 0.36809476734618585 1104 74-75 0.3235939032958477 1104 76-77 0.49819189622114735 1104 78-79 0.2657770852184349 1104 80-81 0.08729700133653395 1104 82-83 -0.11944752275147508 1104 84-85 -0.16023738605421656 1104 86-87 0.16500413551679927 1104 88-89 -0.30966507073875604 1104 90-91 -3.1339393208077553 1104 92-93 -8.624723493127133 1104 94-95 -6.214561973636233 1104 96-97 -3.2361598458038987 1104 98-99 -2.3220885380386527 1104 100-101 -1.81352707202368 1105 1 1.8254003813202644 1105 2 1.0938387172511526 1105 3 0.8192430555636392 1105 4 0.7737248205272351 1105 5 0.6873782832530821 1105 6 0.7076641980349194 1105 7 0.5955674224120528 1105 8 0.5866008209929916 1105 9 0.6223720527607881 1105 10-11 0.6475081636458029 1105 12-13 0.5676948231800552 1105 14-15 0.7133464248829924 1105 16-17 0.6718467408734625 1105 18-19 0.5387377446646582 1105 20-21 0.4823801438924349 1105 22-23 0.3669569160508033 1105 24-25 0.84925951924982 1105 26-27 0.559058291768558 1105 28-29 0.43222719009683885 1105 30-31 0.8324958902494757 1105 32-33 0.838512717159368 1105 34-35 0.8850323597639189 1105 36-37 0.7158033235563934 1105 38-39 0.9584274101539663 1105 40-41 0.7473018906048026 1105 42-43 0.9142813834078538 1105 44-45 0.7313778672067386 1105 46-47 0.8824295843750107 1105 48-49 0.5849868231948108 1105 50-51 0.6231884088277013 1105 52-53 0.44556604778869513 1105 54-55 0.6659791584533465 1105 56-57 0.7052096647032684 1105 58-59 0.5910635974834229 1105 60-61 0.8575114469841054 1105 62-63 0.662901801844324 1105 64-65 0.8307197893635454 1105 66-67 0.8482976248653493 1105 68-69 0.7297165423873864 1105 70-71 0.5585260693015464 1105 72-73 0.5021175951158945 1105 74-75 0.6859837172037793 1105 76-77 0.4332111208352458 1105 78-79 0.13416506114611337 1105 80-81 0.0710645998601045 1105 82-83 -0.2029976681552199 1105 84-85 0.05488563361821619 1105 86-87 0.0892348418754203 1105 88-89 0.3132007498342624 1105 90-91 -3.440898507480888 1105 92-93 -8.968672032427353 1105 94-95 -5.851569802223283 1105 96-97 -3.307844166513595 1105 98-99 -2.503223690029179 1105 100-101 -1.956526537663203 1106 1 1.563051606763473 1106 2 1.0316998168707556 1106 3 0.7434239913199434 1106 4 0.8448408870008421 1106 5 0.649960139226927 1106 6 0.6251796106697469 1106 7 0.6270293776725282 1106 8 -0.2797416169780931 1106 9 -0.42673245045291 1106 10-11 -0.8431367958424332 1106 12-13 -0.3977617036041323 1106 14-15 0.24170466395793255 1106 16-17 0.6605544002718915 1106 18-19 0.703135819472692 1106 20-21 0.6485974645660448 1106 22-23 0.6730493212290511 1106 24-25 0.523635194025573 1106 26-27 0.5717768686602014 1106 28-29 0.45709122593688534 1106 30-31 0.7952174941725048 1106 32-33 0.8356763887466414 1106 34-35 0.7168691624115624 1106 36-37 0.87726114187398 1106 38-39 0.7471522227302714 1106 40-41 0.6888640406042938 1106 42-43 0.7328017704642633 1106 44-45 0.4804430190123625 1106 46-47 0.3923461392096854 1106 48-49 0.354960185318582 1106 50-51 0.5015047739684135 1106 52-53 0.47204752740163514 1106 54-55 0.520176137476021 1106 56-57 0.6561238281831479 1106 58-59 0.6449952067541993 1106 60-61 0.48001086680308447 1106 62-63 0.6136631002389876 1106 64-65 0.49373753785759433 1106 66-67 0.4544561202289472 1106 68-69 0.13726444515913983 1106 70-71 0.5754517153204937 1106 72-73 0.7759009807496042 1106 74-75 0.7946168107289573 1106 76-77 0.2519782109499715 1106 78-79 0.1663393777937756 1106 80-81 0.12206049318751866 1106 82-83 -0.18028433811798905 1106 84-85 -0.2367380660195515 1106 86-87 0.3953570128623305 1106 88-89 2.465689994046727E-4 1106 90-91 -4.061408158665969 1106 92-93 -9.360915264489671 1106 94-95 -6.8060775564686935 1106 96-97 -3.6051062166210563 1106 98-99 -2.4865129632389404 1106 100-101 -1.8198197460137724 1107 1 1.8948098886023637 1107 2 1.1880453943397136 1107 3 0.9713319515057997 1107 4 0.8884757513356831 1107 5 0.857989571669215 1107 6 0.7570869576797179 1107 7 0.6905871721423154 1107 8 0.6317057424011239 1107 9 0.6602814358663949 1107 10-11 0.7033030838482333 1107 12-13 0.8305326631067587 1107 14-15 0.8207871731159244 1107 16-17 0.7962290787510042 1107 18-19 0.7812139888255274 1107 20-21 0.8383629776515349 1107 22-23 0.7448540669989328 1107 24-25 0.6134921548933221 1107 26-27 0.6533134028421088 1107 28-29 0.5844327913326239 1107 30-31 0.8183191597907822 1107 32-33 1.0440343460251924 1107 34-35 1.0738223654626466 1107 36-37 0.8073355687395392 1107 38-39 0.7505693567673504 1107 40-41 0.7003621455736635 1107 42-43 0.9257581768307261 1107 44-45 0.6414733130724883 1107 46-47 0.6834125366427983 1107 48-49 0.6975999603464587 1107 50-51 0.8884525853005414 1107 52-53 0.8356713968586078 1107 54-55 0.8311089048550642 1107 56-57 0.6129081435669832 1107 58-59 0.5257668568845055 1107 60-61 0.3560893427438607 1107 62-63 0.5511841781599003 1107 64-65 0.8223038692157267 1107 66-67 0.8017615822516895 1107 68-69 0.4901143915062498 1107 70-71 0.7014506369767055 1107 72-73 0.6545347085946638 1107 74-75 0.7870123726042202 1107 76-77 0.6986023077048955 1107 78-79 0.7217834281376341 1107 80-81 0.721015186890309 1107 82-83 0.6494729288091037 1107 84-85 0.45674086134553704 1107 86-87 0.604718517046198 1107 88-89 0.6279425606263835 1107 90-91 -3.071389710529548 1107 92-93 -4.724972057262654 1107 94-95 -2.821419904192279 1107 96-97 -1.4395513717386947 1107 98-99 -0.7725862060407316 1107 100-101 -0.5009085560825444 1108 1 1.7177549712304518 1108 2 1.0543151939568425 1108 3 -0.2957720301987905 1108 4 -0.1497575943396683 1108 5 0.23696607607870135 1108 6 0.6004048308076051 1108 7 0.4281873837637278 1108 8 0.5906817931164383 1108 9 0.7697905195541281 1108 10-11 0.6390599899000904 1108 12-13 0.5573674426863775 1108 14-15 0.5418290856137062 1108 16-17 0.6741176783778187 1108 18-19 0.38881678804163755 1108 20-21 0.4881449160867817 1108 22-23 0.4737335462195418 1108 24-25 0.3590731544892236 1108 26-27 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0.2906503136604677 2311 80-81 0.5438300006584633 2311 82-83 0.9087974733559072 2311 84-85 1.2595122414268012 2311 86-87 1.641312556120031 2311 88-89 2.1085149542911736 2311 90-91 2.5662796910542554 2311 92-93 3.669597632884738 2311 94-95 3.3646804525087397 2311 96-97 2.8407140502423793 2311 98-99 2.514216837535823 2311 100-101 2.3796556736504986 2312 1 1.2509182365365774 2312 2 0.5903129263831488 2312 3 -0.15073452801843246 2312 4 -0.6345386862636175 2312 5 -0.02010918992513666 2312 6 -0.13742876416884542 2312 7 0.16016671395734505 2312 8 0.2784979452183052 2312 9 0.06375746530678583 2312 10-11 0.12123555243280038 2312 12-13 -0.03636620461870166 2312 14-15 0.2674417665835236 2312 16-17 -0.06121395427523879 2312 18-19 -0.3240789471755221 2312 20-21 0.34071992044749067 2312 22-23 0.31895528876271584 2312 24-25 0.44200857224606693 2312 26-27 0.4085247780250114 2312 28-29 0.25825683191364845 2312 30-31 0.27426002937511385 2312 32-33 0.4453555684797763 2312 34-35 0.43275453103974115 2312 36-37 0.3583401248414546 2312 38-39 0.09302706132093164 2312 40-41 0.08591716733368315 2312 42-43 0.4977997720629901 2312 44-45 0.3435951895836027 2312 46-47 0.13308739236101275 2312 48-49 0.32127175145028986 2312 50-51 0.33862277187778034 2312 52-53 0.20059737982739279 2312 54-55 0.2870487931975987 2312 56-57 0.20997689957218313 2312 58-59 0.2605738976476424 2312 60-61 0.318110771494716 2312 62-63 0.5205264557084597 2312 64-65 0.5548528763560299 2312 66-67 0.5805967461897161 2312 68-69 0.3896725187003085 2312 70-71 0.3024439676724633 2312 72-73 0.16447704297674548 2312 74-75 0.3991247428448048 2312 76-77 0.529907021747487 2312 78-79 0.6253191170792718 2312 80-81 0.7513620519405144 2312 82-83 0.9523337981422344 2312 84-85 1.3428455747601333 2312 86-87 1.6495924706499459 2312 88-89 2.152051279077501 2312 90-91 2.8291002038747664 2312 92-93 3.7176745559616613 2312 94-95 3.424776606354893 2312 96-97 2.846857212635541 2312 98-99 2.7439176922366784 2312 100-101 2.4245274685222906 2313 1 -7.221830406850195 2313 2 -2.67249895658221 2313 3 -1.6556500935155825 2313 4 -0.9430214886882666 2313 5 -0.645284375984815 2313 6 -0.32839019907211764 2313 7 -0.06451070205690712 2313 8 -0.09704228371449375 2313 9 -0.12004377877313033 2313 10-11 -0.2420390633660574 2313 12-13 -0.39526289489665345 2313 14-15 -0.45797436503212197 2313 16-17 -0.6119199627255938 2313 18-19 -0.8701093001020865 2313 20-21 -0.4836513083058023 2313 22-23 -1.4070049552205504 2313 24-25 -0.5839245739920074 2313 26-27 -0.5593311689807692 2313 28-29 -0.6375208388539235 2313 30-31 -0.35641970979614257 2313 32-33 -0.6437882463304376 2313 34-35 -0.4200835694255787 2313 36-37 -0.3197792226034224 2313 38-39 -0.3519394893604826 2313 40-41 -0.5381715199617929 2313 42-43 -0.41771135699822537 2313 44-45 -0.4433729524431058 2313 46-47 -1.259075663574258 2313 48-49 -0.6003605338734346 2313 50-51 -0.7946532592173128 2313 52-53 -1.0268774037358384 2313 54-55 -0.8239518557675076 2313 56-57 -0.6083586084846502 2313 58-59 -0.7509439385369951 2313 60-61 -0.5818722276906243 2313 62-63 -0.5718643421043907 2313 64-65 -0.49225189353751375 2313 66-67 -0.334494108925373 2313 68-69 -0.4180033922672024 2313 70-71 -0.22901185437302374 2313 72-73 -0.21599687414902746 2313 74-75 -0.09888434957058934 2313 76-77 -0.026306560263751777 2313 78-79 -0.28124975606876035 2313 80-81 -0.07360765726788188 2313 82-83 0.3796950128116059 2313 84-85 0.5988385776735718 2313 86-87 0.9202312007495017 2313 88-89 1.4488768731567276 2313 90-91 2.280256086674431 2313 92-93 3.288709914204208 2313 94-95 2.9495887344081346 2313 96-97 1.9534976272828182 2313 98-99 1.4570358487615387 2313 100-101 1.455702388782429 2314 1 0.673221017043133 2314 2 -0.012091601965302345 2314 3 -1.3801108953737007 2314 4 -0.3094427044491468 2314 5 -0.07916796306636087 2314 6 -3.086075527363082 2314 7 -2.475296668209257 2314 8 -2.272968001999999 2314 9 -3.0158887545046156 2314 10-11 -2.417082725162274 2314 12-13 -1.1686983135610305 2314 14-15 -1.291245655409007 2314 16-17 -2.054067372604088 2314 18-19 -3.040994140301855 2314 20-21 -3.139878761671177 2314 22-23 -3.711219809829238 2314 24-25 -2.7772106589977312 2314 26-27 -1.4702241779206346 2314 28-29 -1.5995665461241941 2314 30-31 -1.7402589997018012 2314 32-33 -1.9773852996039594 2314 34-35 -1.4592737877480175 2314 36-37 -1.7469162735507098 2314 38-39 -3.0762494446088198 2314 40-41 -2.4154976449500936 2314 42-43 -1.504139736851812 2314 44-45 -1.8546424051524681 2314 46-47 -1.295989877152131 2314 48-49 -2.020018343795943 2314 50-51 -2.7751305085884397 2314 52-53 -1.5807229158772742 2314 54-55 -2.1290756770634616 2314 56-57 -1.2584140584425896 2314 58-59 -0.7569197965013821 2314 60-61 -1.1544621877349641 2314 62-63 -0.8760476519810041 2314 64-65 -1.3056550857447995 2314 66-67 -0.4958416759594222 2314 68-69 -0.7192908658391453 2314 70-71 -0.6473008322250315 2314 72-73 -1.6614657499635506 2314 74-75 -1.8914732511471364 2314 76-77 -1.0554193579466045 2314 78-79 -2.0531515924505683 2314 80-81 -0.9248652754701823 2314 82-83 -0.5150438042818699 2314 84-85 -1.5025769884407474 2314 86-87 -0.24656134584326495 2314 88-89 0.9408746772845724 2314 90-91 2.1006548838483816 2314 92-93 3.4316963541554486 2314 94-95 3.182148608851133 2314 96-97 2.172850873564652 2314 98-99 1.9721608750363586 2314 100-101 2.2106581259917863 2315 1 -2.620584203621455 2315 2 -1.748097614066669 2315 3 -2.470586311012209 2315 4 -3.485455359385732 2315 5 -3.8252338445588556 2315 6 -2.9870375642308176 2315 7 -5.578847733327496 2315 8 -2.896892609175268 2315 9 -2.349825758283991 2315 10-11 -3.6141283409749043 2315 12-13 -2.775046582649537 2315 14-15 -1.9276524183520465 2315 16-17 -2.4123096045967216 2315 18-19 -1.9094084653411443 2315 20-21 -1.2413968843656846 2315 22-23 -0.674538107317538 2315 24-25 -2.041324922473379 2315 26-27 -3.5656723260625753 2315 28-29 -2.8566528241402693 2315 30-31 -2.914355632824382 2315 32-33 -3.5968878921549248 2315 34-35 -3.479443515542748 2315 36-37 -2.731672979710929 2315 38-39 -1.497455354048185 2315 40-41 -1.2323665881857266 2315 42-43 -2.8526575961813876 2315 44-45 -6.0645927348618756 2315 46-47 -3.2767436041014086 2315 48-49 -2.1193220203368384 2315 50-51 -3.579025122356221 2315 52-53 -3.4443688259205345 2315 54-55 -1.8730849636128966 2315 56-57 -1.3274185222265587 2315 58-59 -2.891060782291415 2315 60-61 -2.961677715865523 2315 62-63 -4.624977165795919 2315 64-65 -4.0744023912867 2315 66-67 -3.674357807480849 2315 68-69 -1.9461322815682394 2315 70-71 -3.5214521513142927 2315 72-73 -2.2087835374968243 2315 74-75 -1.2822942284155552 2315 76-77 -3.22615867531141 2315 78-79 -1.7177887275769095 2315 80-81 -1.4079216842125568 2315 82-83 -0.9660621207257378 2315 84-85 -2.534083150676903 2315 86-87 -1.716308096138082 2315 88-89 -1.0456118500055709 2315 90-91 -0.1659920444274512 2315 92-93 0.25602489939130635 2315 94-95 0.3035890305034208 2315 96-97 -0.488559454031126 2315 98-99 -0.6455481520063557 2315 100-101 -0.7303959376284563 2316 1 -6.875224892779929 2316 2 -5.922285972825659 2316 3 -3.1493082625054782 2316 4 -3.8951417399007298 2316 5 -1.8686223941271933 2316 6 -2.5323164358367016 2316 7 -2.939320267069885 2316 8 -2.210220268112913 2316 9 -6.521380199745739 2316 10-11 -6.700103853775033 2316 12-13 -5.482974524059841 2316 14-15 -4.2144828318658405 2316 16-17 -4.366988642693116 2316 18-19 -3.314169345683112 2316 20-21 -2.3203882137643532 2316 22-23 -3.0861138745301346 2316 24-25 -2.752834280549564 2316 26-27 -3.899495153969376 2316 28-29 -4.089255968753179 2316 30-31 -6.413532533668995 2316 32-33 -4.505327464517499 2316 34-35 -5.15131690792721 2316 36-37 -3.668400038160325 2316 38-39 -6.096274955032165 2316 40-41 -3.4270323444548367 2316 42-43 -4.4138316348857565 2316 44-45 -2.6754660617725428 2316 46-47 -3.486063331356277 2316 48-49 -4.708824700085792 2316 50-51 -4.124652875763196 2316 52-53 -6.000253517211583 2316 54-55 -4.452288615180056 2316 56-57 -3.607125386686448 2316 58-59 -2.428481862983194 2316 60-61 -2.7902065196592645 2316 62-63 -3.8026898854838898 2316 64-65 -2.5983031330112816 2316 66-67 -3.7964804679618034 2316 68-69 -2.6985842752453415 2316 70-71 -2.1296410525704523 2316 72-73 -1.6302720374921478 2316 74-75 -1.7195890382141776 2316 76-77 -2.659871133104197 2316 78-79 -4.445272756647277 2316 80-81 -5.389317772620888 2316 82-83 -2.928771987626863 2316 84-85 -1.6078085161609827 2316 86-87 -1.9442336056645217 2316 88-89 -0.7055867828949296 2316 90-91 -0.37384328796191113 2316 92-93 -0.7968330318237911 2316 94-95 -1.403105197513753 2316 96-97 -3.1080640979939744 2316 98-99 -4.951874791423453 2316 100-101 -4.502729517267976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10445.0 3 3173.0 4 2902.0 5 3274.0 6 3698.0 7 4351.0 8 4928.0 9 6215.0 10 7456.0 11 9319.0 12 11201.0 13 13321.0 14 15184.0 15 17859.0 16 20917.0 17 22846.0 18 20273.0 19 19015.0 20 19605.0 21 21798.0 22 24386.0 23 27952.0 24 32298.0 25 36856.0 26 43518.0 27 50587.0 28 59382.0 29 69913.0 30 82313.0 31 96164.0 32 111531.0 33 128376.0 34 150075.0 35 179511.0 36 197950.0 37 163815.0 38 51065.0 39 988.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27384129717319 19.980198019801982 13.324207203329028 22.4217534796958 2 15.524072751981672 20.489730657894434 39.98110725706297 24.005089333060923 3 19.028108185666117 25.663820934069875 29.595465958820576 25.712604921443432 4 12.609001115840806 16.68216445564877 37.28945966671718 33.41937476179324 5 13.432989152299966 37.8968168563192 34.091025179680635 14.579168811700205 6 31.258588328483118 36.43391363743335 17.8823205276118 14.425177506471739 7 28.2721019067321 33.157008413184805 21.04295242901788 17.52793725106521 8 26.5807945723417 34.44976241364998 20.19280173925723 18.77664127475109 9 27.08287282032615 16.459275530984655 20.337115616371662 36.12073603231754 10-11 24.71272467985713 26.929863514979424 27.47339809437092 20.884013710792527 12-13 25.84572878297891 24.525783099746928 27.898307896625923 21.730180220648236 14-15 22.655796345374483 25.492880165356862 26.48976321946439 25.36156026980426 16-17 21.786224985060354 29.042760399056494 27.067081742976068 22.103932872907087 18-19 21.7641840917703 27.12408281373799 29.838092941541216 21.2736401529505 20-21 22.3666849633454 26.396021276204824 29.966371240377022 21.27092252007276 22-23 22.0686147586462 26.45569867448109 29.88347862846299 21.59220793840972 24-25 21.821281954355925 26.67270260115839 29.958188897940236 21.547826546545444 26-27 21.525617766746098 26.99977160980613 29.795641051054545 21.67896957239323 28-29 21.457353571897432 27.144128095988684 29.993993238299527 21.404525093814357 30-31 21.504558790327817 26.867191014205183 29.898954617760754 21.72929557770625 32-33 21.31890985132247 27.002062412390558 29.74426660845187 21.9347611278351 34-35 21.893602440622978 26.975868920486175 29.559409190937618 21.571119447953233 36-37 21.861457748390404 26.658805991156022 29.562899526492437 21.916836733961134 38-39 21.417329573685066 27.02475037953569 29.679633394085847 21.878286652693397 40-41 21.620838373985983 26.87951720025781 29.753579581421906 21.746064844334306 42-43 21.6416980282415 26.927789112364042 29.4809142416013 21.949598617793157 44-45 21.51605489503933 27.040278168254687 29.55035151537429 21.89331542133169 46-47 21.706697456267708 27.303080827010984 29.030601474281926 21.95962024243939 48-49 21.591110546672187 27.30507773789353 28.912071749126312 22.191739966307978 50-51 21.582666217277406 27.51808116533186 28.809874150894228 22.08937846649651 52-53 21.94065390524589 27.529424069508067 28.59777330744615 21.932148717799894 54-55 21.41455586635118 27.499378648197077 28.845291302655074 22.24077418279667 56-57 21.50027777283066 27.740399889465372 28.61623169988355 22.14309063782042 58-59 21.597335428375274 27.2618481659526 28.619268736525978 22.52154766914615 60-61 21.304249064325955 27.394968022102955 29.059696764935534 22.241086148635553 62-63 21.34637843171094 27.409839473731818 28.997672063295067 22.246110031262177 64-65 21.14795895658786 27.381485324019607 28.90509263712796 22.56546308226457 66-67 21.19810110024418 27.566181067460565 28.61131683833363 22.624400993961626 68-69 21.30405366210312 27.220542904353927 28.82649471574857 22.64890871779438 70-71 21.253902200165996 27.44165008141849 28.57878064681032 22.725667071605194 72-73 21.35718720167223 27.341741023269815 28.456235396931966 22.844836378125986 74-75 21.196932039627104 27.658098874696226 28.360332790442516 22.784636295234158 76-77 21.357448251290126 27.347569735768385 28.42673577987978 22.868246233061708 78-79 21.417946834176657 27.543127090055968 28.260616600808685 22.77830947495869 80-81 21.419271226208387 27.632282601355477 28.202146292121594 22.746299880314545 82-83 21.46039441787329 27.569250716386723 28.070491533820462 22.899863331919526 84-85 21.349980852537534 27.52207129291193 28.008615065332638 23.119332789217893 86-87 21.33389642551382 27.364584949350434 28.24884077706721 23.052677848068537 88-89 21.435095204234813 27.24586466705452 28.10322710393817 23.215813024772494 90-91 21.264929083792893 27.283895735814312 28.31900575080314 23.13216942958966 92-93 21.730971570205707 26.85486263752899 28.29092811108833 23.123237681176974 94-95 21.340260385689255 26.74331520657487 28.386371453979244 23.530052953756634 96-97 21.727861994519923 26.691744881612777 27.90605239697268 23.67434072689462 98-99 21.708489926798634 26.798059041186583 27.55859084711255 23.934860184902227 100-101 21.81661293643117 26.763235407439268 27.77629858292494 23.643853073204617 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3042.0 1 1915.5 2 873.0 3 970.0 4 883.0 5 831.5 6 1264.5 7 2338.0 8 3566.0 9 3847.0 10 3167.5 11 2530.5 12 2352.5 13 2400.0 14 2494.0 15 2579.5 16 2634.5 17 2786.5 18 3010.0 19 3298.0 20 3661.0 21 4230.0 22 4999.0 23 5885.0 24 7012.0 25 8492.5 26 10500.0 27 13159.0 28 15659.5 29 18518.5 30 21986.5 31 25226.5 32 29290.5 33 32659.0 34 35387.5 35 38530.0 36 42348.0 37 46392.0 38 49356.5 39 50895.0 40 52124.5 41 54648.0 42 57213.5 43 59854.5 44 61862.0 45 63628.5 46 64246.0 47 63869.0 48 65276.5 49 65176.0 50 62981.0 51 60490.0 52 58480.0 53 57211.5 54 54755.5 55 50328.5 56 45378.0 57 41044.0 58 37447.5 59 33524.5 60 28461.5 61 23023.0 62 18003.0 63 13586.0 64 9989.0 65 7654.0 66 5955.5 67 4645.0 68 3460.0 69 2491.0 70 1903.5 71 1493.5 72 1210.0 73 998.0 74 776.5 75 584.0 76 402.5 77 261.0 78 173.5 79 120.0 80 81.5 81 56.0 82 39.0 83 27.5 84 18.0 85 10.5 86 7.0 87 6.5 88 5.5 89 3.5 90 2.5 91 3.0 92 3.0 93 2.5 94 3.5 95 3.5 96 2.5 97 2.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12668676839824358 2 0.11419006454719512 3 0.1195212271992479 4 0.1304701741513133 5 0.11826009194822466 6 0.13012622817376152 7 0.15987755523199157 8 0.14715155406257524 9 0.15724063607076116 10-11 0.15021840569574538 12-13 0.14081721564266306 14-15 0.14505921603246852 16-17 0.14050193182990725 18-19 0.15592217649014595 20-21 0.16027882553913533 22-23 0.19524666659023424 24-25 0.2163420198800775 26-27 0.2303004941357211 28-29 0.22479735849489238 30-31 0.2153675062770141 32-33 0.2166859658576293 34-35 0.20596631622393177 36-37 0.2136764385540511 38-39 0.20160966717494241 40-41 0.20717012714536304 42-43 0.19925936966167182 44-45 0.19235178794583999 46-47 0.20708414065097508 48-49 0.24566341446636783 50-51 0.2476697660020866 52-53 0.24910287424188574 54-55 0.24663792806943122 56-57 0.21949485800763557 58-59 0.22026873645712713 60-61 0.19120530135400068 62-63 0.1844983547917407 64-65 0.1851862467468443 66-67 0.1918358689795123 68-69 0.19163523382594042 70-71 0.19805555874024056 72-73 0.23098838609082467 74-75 0.2341985485479747 76-77 0.24764110383729063 78-79 0.2422239546908499 80-81 0.256956307395985 82-83 0.25669834791282115 84-85 0.23047246712449698 86-87 0.23331002143929927 88-89 0.23631954874287747 90-91 0.4490214736938652 92-93 0.5971188791947077 94-95 0.34176765302729784 96-97 0.26959632207101336 98-99 0.29903236531648764 100-101 0.31677424532520093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1744460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.0998967886644 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6962003776365 44.1484871884546 2 11.741533152708602 13.173975960152681 3 3.9418538010218604 6.634127741799927 4 1.8192143090075532 4.082309398871407 5 0.9536082927338911 2.6748663399592885 6 0.60190411888146 2.026005536755314 7 0.39884655132206304 1.5662675254577736 8 0.28709040992362367 1.2884593892584517 9 0.21066247019651463 1.0636328551742604 >10 1.2024801894695174 12.651527400925081 >50 0.09850863901367081 3.807776806783435 >100 0.04496119150572618 4.400043300050928 >500 0.002195547605345255 0.8625418726500038 >1k 7.318492017817517E-4 0.7549734111757683 >5k 2.0909977193764336E-4 0.8650052725310933 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8739 0.5009573163041858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6009 0.3444618965181202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4395 0.251940428556688 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.598649438794814E-5 0.0 18-19 0.0 0.0 0.0 1.7197298877589626E-4 0.0 20-21 0.0 0.0 0.0 3.7260814234777524E-4 0.0 22-23 0.0 0.0 0.0 7.165541198995678E-4 0.0 24-25 0.0 0.0 0.0 0.001490432569391101 0.0 26-27 0.0 0.0 0.0 0.0024362840076585304 0.0 28-29 2.866216479598271E-5 0.0 0.0 0.007222865528587643 0.0 30-31 5.732432959196542E-5 0.0 0.0 0.022528461529642413 0.0 32-33 5.732432959196542E-5 0.0 0.0 0.058556802678192674 0.0 34-35 5.732432959196542E-5 0.0 0.0 0.11516457815025853 0.0 36-37 5.732432959196542E-5 0.0 0.0 0.20565103241117594 0.0 38-39 5.732432959196542E-5 0.0 0.0 0.3543790055375302 0.0 40-41 5.732432959196542E-5 0.0 0.0 0.517294750237896 0.0 42-43 5.732432959196542E-5 0.0 0.0 0.6776309001066233 0.0 44-45 5.732432959196542E-5 0.0 0.0 0.8742246884422686 0.0 46-47 5.732432959196542E-5 0.0 0.0 1.102375520218291 0.0 48-49 5.732432959196542E-5 0.0 0.0 1.3481535833438427 0.0 50-51 5.732432959196542E-5 0.0 0.0 1.6131639590474989 0.0 52-53 5.732432959196542E-5 0.0 0.0 1.8689737798516446 0.0 54-55 5.732432959196542E-5 0.0 0.0 2.1455923322976735 0.0 56-57 5.732432959196542E-5 0.0 0.0 2.461879320821343 0.0 58-59 5.732432959196542E-5 0.0 0.0 2.807000447129771 0.0 60-61 5.732432959196542E-5 0.0 0.0 3.1674271694392533 0.0 62-63 5.732432959196542E-5 0.0 0.0 3.521003634362496 0.0 64-65 5.732432959196542E-5 0.0 0.0 3.8866468706648476 0.0 66-67 5.732432959196542E-5 0.0 0.0 4.291184664595347 0.0 68-69 5.732432959196542E-5 0.0 0.0 4.73908831386217 0.0 70-71 1.1464865918393085E-4 0.0 0.0 5.218405695745388 0.0 72-73 1.1464865918393085E-4 0.0 0.0 5.690700847253591 0.0 74-75 1.1464865918393085E-4 0.0 0.0 6.151273173360238 0.0 76-77 1.1464865918393085E-4 0.0 0.0 6.644291070015936 0.0 78-79 1.1464865918393085E-4 0.0 0.0 7.190047349896243 0.0 80-81 1.1464865918393085E-4 0.0 0.0 7.747440468683719 0.0 82-83 1.1464865918393085E-4 0.0 0.0 8.277059949783887 0.0 84-85 1.1464865918393085E-4 0.0 0.0 8.776268874035518 0.0 86-87 1.1464865918393085E-4 0.0 0.0 9.285022299164211 0.0 88-89 1.1464865918393085E-4 0.0 0.0 9.834791282115955 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3830 0.0 47.18077 1 GTATCAA 7275 0.0 43.288635 1 TATCAAC 9260 0.0 35.18719 2 ATCAACG 9260 0.0 35.035545 3 TCAACGC 9415 0.0 34.5605 4 CAACGCA 9680 0.0 33.51637 5 AACGCAG 9885 0.0 32.965244 6 ACGCAGA 11395 0.0 28.38875 7 CGCAGAG 11590 0.0 27.86859 8 TACATGG 8595 0.0 27.149265 2 GTACATG 8660 0.0 27.109789 1 ACATGGG 8730 0.0 25.915997 3 TATCACG 110 9.577038E-5 25.870056 2 GCAGAGT 11935 0.0 25.712576 9 TGGTATC 2105 0.0 23.657892 2 CATGGGG 3770 0.0 23.024275 4 GTGGTAT 2285 0.0 22.832073 1 GAGTACT 6905 0.0 22.323626 12-13 GGACCGA 255 5.002221E-10 22.321186 6 AGTACTT 7310 0.0 21.897848 12-13 >>END_MODULE