FastQCFastQC Report
Fri 27 May 2016
SRR939729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744460
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92440.5299061027481283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72650.41646125448562876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58440.33500338213544595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22240.12748930901253108No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18280.1047888744941128No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.10117744172981898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.045.98661
GTATCAA76400.038.3094061
ATCAACG99200.029.2118153
TCAACGC100400.028.9557024
TATCAAC100600.028.9014532
CAACGCA101200.028.5871245
AACGCAG104350.027.7526076
CGCAGAG122450.023.574118
ACGCAGA122750.023.5541827
GTACATG84650.023.1061921
TACATGG83750.023.0691472
TGGTATC24400.022.016462
GCAGAGT129950.021.9236419
ACATGGG85250.021.8874873
TACGCGC804.0408202E-521.35975364-65
GTGGTAT28000.021.0406511
GAGTACT76650.020.88846812-13
GGACCGA2551.3182216E-820.3129836
AGTACTT79900.020.0977512-13
AGAGTAC119350.019.92519410-11
Produced by FastQC (version 0.11.5)