##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939729_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1744460 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.165354321681207 31.0 31.0 33.0 30.0 34.0 2 31.383019387088268 31.0 31.0 34.0 30.0 34.0 3 31.431424050995723 31.0 31.0 34.0 28.0 34.0 4 35.28444045721885 37.0 35.0 37.0 33.0 37.0 5 35.00089196656845 35.0 35.0 37.0 32.0 37.0 6 35.04212764981713 37.0 35.0 37.0 32.0 37.0 7 34.955930775139585 36.0 35.0 37.0 32.0 37.0 8 34.89135205163775 36.0 35.0 37.0 32.0 37.0 9 36.45020178164016 38.0 35.0 39.0 32.0 39.0 10-11 36.289273184825106 38.0 35.0 39.0 32.0 39.0 12-13 36.21664354585373 38.0 35.0 39.0 32.0 39.0 14-15 37.16373204315376 39.0 36.0 40.0 31.5 41.0 16-17 37.08564885408666 39.0 36.0 40.0 31.0 41.0 18-19 37.0727098930328 39.0 36.0 40.0 31.0 41.0 20-21 36.99482533276773 39.0 36.0 40.0 31.0 41.0 22-23 36.833966671634776 39.0 35.5 40.0 31.0 41.0 24-25 36.64736766678514 38.0 35.0 40.0 30.0 41.0 26-27 36.30789413342811 38.0 35.0 40.0 30.0 41.0 28-29 36.041610584364214 38.0 34.0 40.0 29.0 41.0 30-31 35.74440600529677 38.0 34.0 40.0 28.5 41.0 32-33 35.64686980498263 38.0 34.0 40.0 28.5 41.0 34-35 35.673645139470096 38.0 34.0 40.0 29.0 41.0 36-37 35.60859234376254 38.0 34.0 40.0 28.0 41.0 38-39 35.44240710592389 38.0 34.0 40.0 27.0 41.0 40-41 35.23110303475001 38.0 34.0 40.0 27.0 41.0 42-43 34.97709950357131 38.0 33.5 40.0 26.0 41.0 44-45 34.671292835605286 38.0 33.0 40.0 25.5 41.0 46-47 34.30171743691457 38.0 33.0 40.0 24.0 41.0 48-49 33.93286461139837 37.0 33.0 40.0 23.5 41.0 50-51 33.620487715396166 37.0 32.5 40.0 23.0 41.0 52-53 33.30133279066301 37.0 32.0 40.0 22.0 41.0 54-55 32.947907088726595 37.0 31.5 39.5 19.5 41.0 56-57 31.618181844238332 35.5 29.5 39.0 13.0 40.0 58-59 28.167241725233026 33.5 22.0 38.0 4.0 40.0 60-61 28.1840893457001 33.5 22.0 38.0 2.0 40.0 62-63 29.83056877199821 34.0 27.5 38.0 2.0 40.0 64-65 29.210794744505463 34.0 27.0 38.0 2.0 40.0 66-67 23.689801715143943 26.5 15.5 35.0 2.0 38.0 68-69 16.212334189376655 13.0 7.0 27.0 2.0 34.5 70-71 15.073497242699748 11.5 6.5 24.0 2.0 32.0 72-73 16.90394334063263 13.5 7.0 28.0 2.0 34.0 74-75 17.623625649198033 17.0 6.5 29.0 2.0 34.0 76-77 18.89991401350561 21.5 7.0 30.0 2.0 34.0 78-79 21.8829872854637 27.5 7.5 33.5 2.0 35.5 80-81 24.334173325842954 30.5 15.0 34.0 2.0 36.0 82-83 25.169458170436698 31.5 19.0 34.5 2.0 36.0 84-85 25.120801566100685 32.0 18.0 35.0 2.0 36.0 86-87 24.786928333123143 32.0 16.5 34.0 2.0 35.0 88-89 24.280075209520426 31.5 8.0 34.0 2.0 35.0 90-91 22.676151072538204 30.5 2.0 34.0 2.0 35.0 92-93 20.53361355376449 29.0 2.0 34.0 2.0 35.0 94-95 16.180669662818293 10.0 2.0 32.0 2.0 34.0 96-97 14.815586198594406 2.0 2.0 31.5 2.0 34.0 98-99 13.689930694885524 2.0 2.0 30.5 2.0 34.0 100-101 13.042002109535328 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2965056714538541 1101 2 0.30442285408293657 1101 3 0.18565680660970685 1101 4 0.2544477461846455 1101 5 0.42029869178179524 1101 6 0.25819629505262753 1101 7 0.20244920113979248 1101 8 0.16906804045201085 1101 9 0.47115208251633334 1101 10-11 0.32578586790439346 1101 12-13 0.356265464006988 1101 14-15 0.36937235477397223 1101 16-17 0.42618606652461466 1101 18-19 0.21106064498702182 1101 20-21 0.518148687162352 1101 22-23 0.655880189840147 1101 24-25 0.32913845723084734 1101 26-27 0.48525021905809496 1101 28-29 0.12050585366607436 1101 30-31 0.2985083321051576 1101 32-33 0.44287960349190314 1101 34-35 0.562008897267674 1101 36-37 0.6010300481130031 1101 38-39 0.7289475279392974 1101 40-41 0.6664697255365795 1101 42-43 0.9159968461391585 1101 44-45 0.6416719210151385 1101 46-47 0.7606798917304829 1101 48-49 0.32104275363828094 1101 50-51 0.6510399566484679 1101 52-53 0.531567220871203 1101 54-55 0.6307448546575003 1101 56-57 1.1905571640329065 1101 58-59 -8.301372568234573 1101 60-61 -9.85428006746616 1101 62-63 -3.1965801415338895 1101 64-65 -0.6621811488779663 1101 66-67 -1.8334969335676696 1101 68-69 -4.0646982816920385 1101 70-71 -4.499275406147566 1101 72-73 -6.6731639002029475 1101 74-75 -7.586914327766955 1101 76-77 -8.973105384867232 1101 78-79 -11.697840466686728 1101 80-81 -8.452948106841284 1101 82-83 -5.406897562768673 1101 84-85 -4.554126356165892 1101 86-87 -4.33644977548531 1101 88-89 -4.581960939089338 1101 90-91 -9.260522612630492 1101 92-93 -13.520094342721764 1101 94-95 -11.443762798689953 1101 96-97 -11.02075437965385 1101 98-99 -10.408511361956602 1101 100-101 -10.087358375550021 1102 1 0.4115402525090417 1102 2 0.49358723426091444 1102 3 0.412053400576049 1102 4 0.5017273253372068 1102 5 0.6268487308356896 1102 6 0.5396680594207908 1102 7 0.5263344961802829 1102 8 0.6540781877886701 1102 9 0.7191401393681218 1102 10-11 0.6781316884701027 1102 12-13 0.6505040132048805 1102 14-15 0.8903008193884716 1102 16-17 0.8228684882666144 1102 18-19 0.6685861969153493 1102 20-21 0.6368983950926292 1102 22-23 0.762274096765033 1102 24-25 0.7538896138269564 1102 26-27 0.8516571105683823 1102 28-29 0.9702943951867056 1102 30-31 0.9417188793269986 1102 32-33 0.8269241683247017 1102 34-35 0.7617616393851279 1102 36-37 0.9523139866148114 1102 38-39 0.6721374633835495 1102 40-41 0.8512397081125371 1102 42-43 1.0087732108558 1102 44-45 0.6697435765664679 1102 46-47 0.6883897146194045 1102 48-49 0.9285632152085839 1102 50-51 0.8387185364218865 1102 52-53 0.9966110647090218 1102 54-55 0.8816198120987693 1102 56-57 1.342247747037451 1102 58-59 -8.672365788879059 1102 60-61 -10.080275274184764 1102 62-63 -3.3413140409817927 1102 64-65 -0.4357182133602855 1102 66-67 -1.7785239875688568 1102 68-69 -4.227149130530101 1102 70-71 -4.648391185923657 1102 72-73 -7.026502340584006 1102 74-75 -7.752205763281296 1102 76-77 -9.038657930713832 1102 78-79 -11.737753046046244 1102 80-81 -8.197421313199392 1102 82-83 -4.767076693302144 1102 84-85 -4.1037777918137515 1102 86-87 -3.8217494890900845 1102 88-89 -3.918303425053299 1102 90-91 -9.205733253314635 1102 92-93 -14.027352275335437 1102 94-95 -11.757846233830449 1102 96-97 -11.039821942891118 1102 98-99 -10.420555065600965 1102 100-101 -9.927736430599325 1103 1 0.3134180139228846 1103 2 0.33666879678283124 1103 3 0.29936141656595083 1103 4 0.290544914228434 1103 5 0.3650316328624825 1103 6 0.29417398550256735 1103 7 0.3434149366038781 1103 8 0.30606114592038125 1103 9 0.3557468439056066 1103 10-11 0.4615798615537656 1103 12-13 0.3432857650808927 1103 14-15 0.5065914077887825 1103 16-17 0.33095110275571216 1103 18-19 0.5003503458739047 1103 20-21 0.34854542503862973 1103 22-23 0.4820870219351008 1103 24-25 0.4349156324818395 1103 26-27 0.31798977765583203 1103 28-29 0.4234730893447818 1103 30-31 0.5525743039710846 1103 32-33 0.6488568073596994 1103 34-35 0.5250558746049876 1103 36-37 0.3176183557851573 1103 38-39 0.3026690574644775 1103 40-41 0.44232253299505686 1103 42-43 0.4733745152951059 1103 44-45 0.4493696022344622 1103 46-47 0.19369776567487662 1103 48-49 0.25611511225605454 1103 50-51 0.30875062142083465 1103 52-53 0.38934986125857307 1103 54-55 0.2709221825314714 1103 56-57 0.7315413510770092 1103 58-59 -6.1695210943001975 1103 60-61 -8.728111728354062 1103 62-63 -3.061464472288339 1103 64-65 -0.5572335720810919 1103 66-67 -2.0192973587730094 1103 68-69 -4.128755133756135 1103 70-71 -4.574683352274734 1103 72-73 -7.199454511142953 1103 74-75 -7.966807067720598 1103 76-77 -8.8840582042253 1103 78-79 -11.744038549938523 1103 80-81 -8.753797987580484 1103 82-83 -5.341946074044131 1103 84-85 -4.403075603722023 1103 86-87 -4.204595410963986 1103 88-89 -4.3905840898965565 1103 90-91 -9.27756069563177 1103 92-93 -13.617220954765973 1103 94-95 -11.391446065515698 1103 96-97 -10.921506317671959 1103 98-99 -10.434182423760724 1103 100-101 -10.072189057010712 1104 1 0.10120607966262796 1104 2 0.10474609545475388 1104 3 0.16681980272946717 1104 4 0.18191115205104325 1104 5 -0.02518808780949655 1104 6 0.1068024548048001 1104 7 0.22419806864159142 1104 8 0.14300544331380394 1104 9 0.15446348516089614 1104 10-11 0.2430486199831634 1104 12-13 0.14850767108331553 1104 14-15 0.12791258789606985 1104 16-17 0.24448894839974145 1104 18-19 0.3860692234266381 1104 20-21 0.21350979381470836 1104 22-23 0.304786522891213 1104 24-25 0.30544970897177137 1104 26-27 0.16122381188161938 1104 28-29 0.21963561061509296 1104 30-31 0.2227837783522233 1104 32-33 0.21427908580590582 1104 34-35 0.09608981237812486 1104 36-37 0.017405400261559123 1104 38-39 0.08267768338927795 1104 40-41 0.314291138283032 1104 42-43 -0.09478339298064498 1104 44-45 -0.21318640737617756 1104 46-47 -0.3099516640476452 1104 48-49 -0.21909905734001 1104 50-51 -0.21528056076841295 1104 52-53 -0.26457036672740486 1104 54-55 -0.17516350357814048 1104 56-57 0.455278439235979 1104 58-59 -6.621316647381132 1104 60-61 -8.659382725196512 1104 62-63 -3.0496273859084013 1104 64-65 -0.7371629587412976 1104 66-67 -2.113662523884461 1104 68-69 -4.202095328402002 1104 70-71 -4.735600604460904 1104 72-73 -7.570462676922263 1104 74-75 -8.294864354631649 1104 76-77 -9.011330863746007 1104 78-79 -11.762276322545876 1104 80-81 -8.96037963070857 1104 82-83 -5.60877059417297 1104 84-85 -4.692354334085898 1104 86-87 -4.513653184946488 1104 88-89 -4.614937710501074 1104 90-91 -9.63842354294665 1104 92-93 -14.206976010235861 1104 94-95 -11.76980429815314 1104 96-97 -11.189791210715779 1104 98-99 -10.410143187823408 1104 100-101 -9.85307806578425 1105 1 0.02488594141573941 1105 2 -0.005387110035009357 1105 3 0.040614572590435216 1105 4 0.12002836244209192 1105 5 -0.008416174565461176 1105 6 -0.008022545572956119 1105 7 0.13308617300809544 1105 8 0.17652454361009973 1105 9 0.1112304256930301 1105 10-11 0.13698321645857447 1105 12-13 0.14303700115461737 1105 14-15 0.08031844635510765 1105 16-17 0.24168664666602524 1105 18-19 0.0075332062941342315 1105 20-21 0.011213829541304676 1105 22-23 0.1452139837997919 1105 24-25 0.15065378934288276 1105 26-27 0.09448885055750367 1105 28-29 0.12000554191748591 1105 30-31 0.21703528218547774 1105 32-33 0.001957681028521563 1105 34-35 -5.885671771608258E-4 1105 36-37 0.0684313311683411 1105 38-39 0.09816383466137069 1105 40-41 -0.03356723009051876 1105 42-43 0.337474396800026 1105 44-45 0.12026105564786604 1105 46-47 0.26042354217567265 1105 48-49 0.1992653600216272 1105 50-51 0.03396700747931192 1105 52-53 0.07194726510784477 1105 54-55 -0.006544955912225703 1105 56-57 0.7236503431390098 1105 58-59 -6.7068081422580015 1105 60-61 -8.432468746243995 1105 62-63 -2.9906626303474155 1105 64-65 -1.02068961426286 1105 66-67 -2.426053230682651 1105 68-69 -4.517426391504619 1105 70-71 -4.83227932743552 1105 72-73 -6.197029527615902 1105 74-75 -6.842878498013338 1105 76-77 -8.767541945750423 1105 78-79 -11.808684293720631 1105 80-81 -8.741306258481853 1105 82-83 -5.33404783659817 1105 84-85 -4.531325353615472 1105 86-87 -4.244968426033726 1105 88-89 -4.380137106632521 1105 90-91 -9.448056935943367 1105 92-93 -11.781881260842685 1105 94-95 -10.841139017984784 1105 96-97 -10.795297870990268 1105 98-99 -10.279952576358424 1105 100-101 -9.788452385985686 1106 1 0.015128810234291024 1106 2 0.13310663914516851 1106 3 0.1377116494871231 1106 4 0.09142291580360506 1106 5 0.1410243291325628 1106 6 0.09034588450766279 1106 7 0.1610014564895863 1106 8 0.13497551074663505 1106 9 -0.03470780268639828 1106 10-11 0.18356468900844192 1106 12-13 0.1246891548874558 1106 14-15 0.3354563276349367 1106 16-17 0.19067057312934566 1106 18-19 0.1995226722248944 1106 20-21 0.21186413678499605 1106 22-23 -0.003336068164244921 1106 24-25 0.11391016881153604 1106 26-27 0.1318197151785725 1106 28-29 0.03811727973620549 1106 30-31 0.22907328151952555 1106 32-33 0.14177575644196594 1106 34-35 -0.014683938846168587 1106 36-37 0.10381278757404289 1106 38-39 0.11646526644722144 1106 40-41 0.15609949375340904 1106 42-43 0.08444736540970865 1106 44-45 0.2787614642970979 1106 46-47 0.23420591879891361 1106 48-49 0.2394396161286565 1106 50-51 0.3242719304413626 1106 52-53 0.16501939926586573 1106 54-55 0.13736970198277731 1106 56-57 0.7381422559201418 1106 58-59 -6.632105548092099 1106 60-61 -8.766740795645148 1106 62-63 -2.938349473859983 1106 64-65 -0.6648555157632607 1106 66-67 -1.79040489100937 1106 68-69 -4.241322281805001 1106 70-71 -4.526022958056972 1106 72-73 -6.186095890234082 1106 74-75 -6.8425253443511345 1106 76-77 -8.46832746039621 1106 78-79 -11.479395242493169 1106 80-81 -8.37246046555098 1106 82-83 -5.152648439986429 1106 84-85 -4.357818436848746 1106 86-87 -4.11731548527273 1106 88-89 -4.057609939025795 1106 90-91 -9.320134589388633 1106 92-93 -11.900523738165173 1106 94-95 -11.095682087596415 1106 96-97 -11.072869586408956 1106 98-99 -10.303249114443524 1106 100-101 -9.815317794079967 1107 1 0.07089874241492566 1107 2 0.12200921289807098 1107 3 0.05430387166823181 1107 4 0.07499403099080837 1107 5 0.009584264431936163 1107 6 0.029686403275597684 1107 7 0.06479786095308526 1107 8 0.11043843210682525 1107 9 0.22980094299890652 1107 10-11 0.073082817341934 1107 12-13 0.21913039836585568 1107 14-15 0.18810699182071033 1107 16-17 0.12664047465118244 1107 18-19 0.19722722717020957 1107 20-21 0.1462096508219588 1107 22-23 0.07226931086830746 1107 24-25 0.1288502520131587 1107 26-27 0.26640785656847044 1107 28-29 0.2907214271310039 1107 30-31 0.22240578671302558 1107 32-33 0.1961176759996377 1107 34-35 0.028188948736499242 1107 36-37 0.004695813702731755 1107 38-39 0.17097660227202027 1107 40-41 0.07391886059645003 1107 42-43 0.1813291546146445 1107 44-45 0.4327664014201389 1107 46-47 0.14044921218771833 1107 48-49 0.12677462688566266 1107 50-51 -0.20698021699571711 1107 52-53 0.10084895751493406 1107 54-55 0.14022599152865922 1107 56-57 0.42986808172533486 1107 58-59 -6.615374568437911 1107 60-61 -8.78975259928086 1107 62-63 -3.1571763521075695 1107 64-65 -0.7467874426684666 1107 66-67 -2.090947763104051 1107 68-69 -4.22526976011919 1107 70-71 -4.75625755069146 1107 72-73 -6.344391472724739 1107 74-75 -6.975748376505868 1107 76-77 -9.324739905752196 1107 78-79 -12.335915786528183 1107 80-81 -8.724973958063362 1107 82-83 -5.256797949181422 1107 84-85 -4.317249716219074 1107 86-87 -3.932607351706107 1107 88-89 -3.9390127374336537 1107 90-91 -8.656813079073007 1107 92-93 -11.85907833460558 1107 94-95 -11.047481705947288 1107 96-97 -11.238652522147067 1107 98-99 -10.466301303111399 1107 100-101 -9.806143414259797 1108 1 -8.978412099480693E-5 1108 2 0.045915858018108935 1108 3 0.08532655599206151 1108 4 0.07302507520638102 1108 5 -0.10378967837414876 1108 6 -0.009520846910973546 1108 7 0.1196102680274862 1108 8 0.030386403382600236 1108 9 0.0974106783529507 1108 10-11 0.16737161521002974 1108 12-13 0.05101421728651445 1108 14-15 0.17859883090076778 1108 16-17 0.1681065835047164 1108 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3.9890162799960933 2311 78-79 4.943178621492567 2311 80-81 2.909508958909452 2311 82-83 1.77463358055099 2311 84-85 1.408001599429923 2311 86-87 1.291220378344068 2311 88-89 1.455726315047869 2311 90-91 2.9941293840480014 2311 92-93 4.844976784493621 2311 94-95 8.290203434754936 2311 96-97 9.076167722205689 2311 98-99 9.357533413380372 2311 100-101 8.634801725688833 2312 1 -0.31365816018936954 2312 2 -0.222442767483372 2312 3 -0.3708046144248236 2312 4 -0.24022714876012685 2312 5 -0.2138433152135022 2312 6 -0.3173548779592892 2312 7 -0.3801171870571167 2312 8 -0.34528950800759617 2312 9 -0.26675293581066484 2312 10-11 -0.3485450078494523 2312 12-13 -0.2999745756665604 2312 14-15 -0.46501598682119294 2312 16-17 -0.45599401827445973 2312 18-19 -0.277722454750446 2312 20-21 -0.3744721787779426 2312 22-23 -0.4332060183579358 2312 24-25 -0.3804205204831774 2312 26-27 -0.5116943197473347 2312 28-29 -0.7206015144592115 2312 30-31 -0.6046028544947433 2312 32-33 -0.351167581195611 2312 34-35 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9.185549588936697 2313 1 -0.28802317544695555 2313 2 -0.38217793448832893 2313 3 -0.35685876849190024 2313 4 -0.3993392402227798 2313 5 -0.5165881269445762 2313 6 -0.4882406808119697 2313 7 -0.510720552010234 2313 8 -0.4200827366780473 2313 9 -0.519459258387748 2313 10-11 -0.46455442474739783 2313 12-13 -0.35836892207584725 2313 14-15 -0.6926883940675097 2313 16-17 -0.4878394782802573 2313 18-19 -0.6030239818896135 2313 20-21 -0.44723307655903 2313 22-23 -0.5939957536630232 2313 24-25 -0.5452680136828505 2313 26-27 -0.7847675319865388 2313 28-29 -0.6077596858964611 2313 30-31 -0.6557934293121264 2313 32-33 -0.6544940451642489 2313 34-35 -0.6014098408386275 2313 36-37 -0.8895680812365683 2313 38-39 -1.1079362785526428 2313 40-41 -1.1931950286254605 2313 42-43 -0.9410443575285825 2313 44-45 -0.8846005762804552 2313 46-47 -0.7094918531935122 2313 48-49 -0.8007270873421675 2313 50-51 -0.5520031399708003 2313 52-53 -0.6542776025510975 2313 54-55 -0.6191419223203667 2313 56-57 0.4717886183132656 2313 58-59 4.074094790506166 2313 60-61 3.716472827317549 2313 62-63 1.4147509859983032 2313 64-65 1.1869608663491427 2313 66-67 1.911759193554964 2313 68-69 0.21447824740006993 2313 70-71 2.4225428419686015 2313 72-73 7.576017908226344 2313 74-75 9.384645423073696 2313 76-77 8.400307841378524 2313 78-79 6.374267933589554 2313 80-81 3.6308168479778438 2313 82-83 2.2050674551488356 2313 84-85 1.6146723911673568 2313 86-87 1.6273674759511856 2313 88-89 1.6302470128924789 2313 90-91 2.979439644600589 2313 92-93 4.675764310555785 2313 94-95 8.146987104997812 2313 96-97 8.938030062208178 2313 98-99 9.220005884142422 2313 100-101 8.374613750183814 2314 1 -0.31995481636803547 2314 2 -0.4360871340983117 2314 3 -0.34234938774638835 2314 4 -0.30546690160398526 2314 5 -0.23779279484732285 2314 6 -0.3565101786706677 2314 7 -0.23181668211446294 2314 8 -0.41503827056590836 2314 9 -0.5438793660464825 2314 10-11 -0.40984404874316027 2314 12-13 -0.4467307996541834 2314 14-15 -0.5042506297308762 2314 16-17 -0.4721971781998562 2314 18-19 -0.7391894341588525 2314 20-21 -0.5963417360514427 2314 22-23 -0.4829059713754944 2314 24-25 -0.5851085617897667 2314 26-27 -0.6467076017796316 2314 28-29 -0.5454078601740733 2314 30-31 -0.804947985642201 2314 32-33 -0.7395697390661198 2314 34-35 -0.8834512903175664 2314 36-37 -0.7342721692245391 2314 38-39 -0.5992206634318649 2314 40-41 -0.608830660956805 2314 42-43 -0.5716989002540487 2314 44-45 -0.6707090536805183 2314 46-47 -0.6665238915782083 2314 48-49 -0.7358992244035818 2314 50-51 -0.4562227188142387 2314 52-53 -0.8027650849735011 2314 54-55 -0.7019767442361484 2314 56-57 0.7177059675272055 2314 58-59 4.037497070756743 2314 60-61 3.9586921173248655 2314 62-63 1.580297440923573 2314 64-65 1.7261790413772573 2314 66-67 1.915983646134265 2314 68-69 -0.3607351579449549 2314 70-71 2.1788929759365327 2314 72-73 8.168983821676566 2314 74-75 9.788189406446783 2314 76-77 8.923739896268156 2314 78-79 6.7646789928437165 2314 80-81 4.055938026094349 2314 82-83 2.648108134099111 2314 84-85 2.1399995219990586 2314 86-87 2.1619953692085936 2314 88-89 2.3779558337862703 2314 90-91 3.6539924942859052 2314 92-93 5.579174191442281 2314 94-95 8.952626406086154 2314 96-97 9.611887283275752 2314 98-99 9.57111504775254 2314 100-101 8.582978516123395 2315 1 -0.5859601071514398 2315 2 -0.4240669573250848 2315 3 -0.5490626772750318 2315 4 -0.3528249487446331 2315 5 -0.5638442835301802 2315 6 -0.6386310547868277 2315 7 -0.794904996595676 2315 8 -0.7626249587267324 2315 9 -0.9347817012714543 2315 10-11 -0.7509296490558413 2315 12-13 -0.6249337449799626 2315 14-15 -0.8419111439467102 2315 16-17 -0.8756610141687915 2315 18-19 -0.9333893667240076 2315 20-21 -0.8214565178695352 2315 22-23 -1.0733582377397681 2315 24-25 -0.9063391302386492 2315 26-27 -0.7843847230835124 2315 28-29 -0.7708831332264765 2315 30-31 -0.8755682016685427 2315 32-33 -0.8206000830676956 2315 34-35 -0.8365872621090062 2315 36-37 -0.9080837978766354 2315 38-39 -0.8399954260259932 2315 40-41 -0.6333123604490325 2315 42-43 -0.8490221731588292 2315 44-45 -0.769018317919425 2315 46-47 -0.7296598465948136 2315 48-49 -0.9307173096902019 2315 50-51 -0.8672156046061232 2315 52-53 -0.8508253267659924 2315 54-55 -1.0618315412522463 2315 56-57 0.2752545600345577 2315 58-59 3.7262384715562327 2315 60-61 3.445344883243763 2315 62-63 1.0202336294458902 2315 64-65 0.8451034053173991 2315 66-67 1.6513347686224407 2315 68-69 5.336934146285156 2315 70-71 4.761997863956113 2315 72-73 8.645463152561891 2315 74-75 9.583297864151547 2315 76-77 8.431141121837701 2315 78-79 5.99405995105592 2315 80-81 3.2356607715982726 2315 82-83 1.9180377301107256 2315 84-85 1.6443126679351039 2315 86-87 1.4450918698099748 2315 88-89 1.5788655331644286 2315 90-91 2.896505633176517 2315 92-93 4.659140675319147 2315 94-95 8.348078915685551 2315 96-97 8.945384450844625 2315 98-99 9.027852409171421 2315 100-101 8.096425560481096 2316 1 -0.3663198388959401 2316 2 -0.42142929866804835 2316 3 -0.5427079776594574 2316 4 -0.4005609583973424 2316 5 -0.48553201093470477 2316 6 -0.6717028047143359 2316 7 -0.6134845893486656 2316 8 -0.6276834642405618 2316 9 -0.6595223782705091 2316 10-11 -0.6822448305585596 2316 12-13 -0.6650785356041169 2316 14-15 -0.7650336828334687 2316 16-17 -0.9566633244584679 2316 18-19 -0.9219052628264421 2316 20-21 -0.7870032708368981 2316 22-23 -0.7748052086413395 2316 24-25 -2.2801439996184243 2316 26-27 -1.5764380748742113 2316 28-29 -1.3092021402695835 2316 30-31 -0.9063221792016805 2316 32-33 -1.048474148896922 2316 34-35 -1.0355817716635016 2316 36-37 -0.9964734230859165 2316 38-39 -1.1795790126805628 2316 40-41 -1.0645845163140848 2316 42-43 -1.0477641409270149 2316 44-45 -0.9768948156325195 2316 46-47 -0.9800110667457744 2316 48-49 -1.0702681594465417 2316 50-51 -1.0975346490628297 2316 52-53 -1.0608353349034516 2316 54-55 -1.006137659310852 2316 56-57 0.12155762888492916 2316 58-59 3.5400852441657342 2316 60-61 3.4466955141305604 2316 62-63 1.2433898405708277 2316 64-65 1.0704408292041094 2316 66-67 6.141969209710055 2316 68-69 8.588149620549277 2316 70-71 5.566772774397316 2316 72-73 8.957900367867797 2316 74-75 9.9165164624511 2316 76-77 8.441502817796096 2316 78-79 6.082887315361475 2316 80-81 2.9485375966803495 2316 82-83 1.9095205245323612 2316 84-85 1.712147262418469 2316 86-87 1.6495843075864833 2316 88-89 1.6933043759618442 2316 90-91 3.165247507410708 2316 92-93 4.796473834055686 2316 94-95 8.435968134021955 2316 96-97 8.86729011245734 2316 98-99 8.981893545422393 2316 100-101 8.404999821796372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 48.0 5 140.0 6 283.0 7 683.0 8 1483.0 9 2606.0 10 4093.0 11 6189.0 12 8530.0 13 11330.0 14 14906.0 15 20653.0 16 27090.0 17 34311.0 18 36914.0 19 37706.0 20 39097.0 21 39185.0 22 37661.0 23 36113.0 24 36618.0 25 37668.0 26 48802.0 27 69210.0 28 95621.0 29 121368.0 30 136752.0 31 142470.0 32 144694.0 33 143690.0 34 138061.0 35 123751.0 36 90596.0 37 45552.0 38 10311.0 39 259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.391847301020306 21.005856939312018 15.150867073565797 22.45142868610188 2 16.08811576788866 22.259510074312637 38.35158138646986 23.30079277132884 3 19.259244821598603 26.07272721018981 30.596322307440516 24.07170566077107 4 13.543618082386526 17.989119842243444 36.66624628824966 31.801015787120367 5 13.048194042623484 38.55666803559834 34.219123899023316 14.176014022754856 6 31.0777725357833 37.46594216315774 17.186531755690446 14.269753545368507 7 27.569161803652708 33.60753470988157 21.0616465840432 17.761656902422526 8 26.352670200526585 35.38143961129452 19.78025253703588 18.48563765114302 9 26.452999782167545 16.511871868658496 20.272290565561836 36.76283778361212 10-11 24.700681013035553 27.443392224527933 27.303721495477113 20.552205266959405 12-13 25.6642456691469 24.799823441064856 28.00594453297869 21.52998635680956 14-15 22.32913231141046 25.92480809925613 26.497450638637304 25.248608950696106 16-17 21.461655755935936 29.37166802334247 27.148573197436455 22.018103023285143 18-19 21.53515477433198 27.316054465522804 30.079887964238445 21.068902795906773 20-21 22.140949061600725 26.541909817364683 30.196737099159627 21.120404021874965 22-23 21.857537576098046 26.643861137543993 30.14141912110338 21.357182165254578 24-25 21.712478360065578 26.860489779072033 30.133078431147748 21.29395342971464 26-27 21.419207089873087 27.21168728431721 29.97916260619332 21.389943019616386 28-29 21.373518715547522 27.289197859245874 30.14087106863437 21.196412356572225 30-31 21.436664991717457 27.014303187209542 30.086368330790336 21.462663490282665 32-33 21.2647893484303 27.11153875146786 29.920350550443647 21.70332134965819 34-35 21.750807201686488 27.091336680133164 29.779658660811915 21.37819745736843 36-37 21.853106405182288 26.700964869905263 29.819769583734107 21.62615914117834 38-39 21.44218725407196 27.081620945493246 29.902240871222794 21.573950929212003 40-41 21.58153277698713 26.957284354683893 29.96021211033499 21.50097075799399 42-43 21.541235901582002 27.034530014506963 29.67898325105075 21.74525083286028 44-45 21.495038794849602 27.120069794318347 29.60363971750847 21.78125169332358 46-47 21.66751149114196 27.439315990348355 29.159346128792535 21.733826389717148 48-49 21.584863877364192 27.407128773845507 28.991912374162144 22.016094974628153 50-51 21.58493906804076 27.557380569374878 28.91403299990166 21.943647362682697 52-53 21.98405301556713 27.558875208095447 28.622150844723453 21.834920931613965 54-55 21.47713476905436 27.48517576235353 28.922693231088974 22.114996237503135 56-57 21.77365627555547 27.483471557128148 28.89381065679481 21.84906151052157 58-59 21.104859737041625 26.733118978493632 30.264541764520963 21.897479519943783 60-61 21.890396346065653 27.00075764219449 29.384383369825713 21.724462641914148 62-63 21.301167813920213 27.490375222992153 28.990728341672135 22.2177286214155 64-65 20.793041609048963 27.37361735452421 29.390899651466118 22.44244138496071 66-67 22.497930827828874 26.087395432256976 30.07131916453446 21.34335457537969 68-69 24.17293911779386 24.3826173555055 31.13353093668472 20.310912590015917 70-71 23.045928242931808 25.175602983996786 30.901872052323487 20.876596720747916 72-73 22.721388162030202 25.37503471866914 30.725539783940242 21.17803733536042 74-75 22.86457538037251 25.809497277683295 29.928465606492065 21.39746173545213 76-77 20.60633863631223 26.760257062341854 30.382235532993747 22.251168768352173 78-79 21.010118576483315 27.306710369964506 29.149953773512383 22.533217280039803 80-81 21.091245637608516 27.76372691543954 28.335223129732135 22.809804317219808 82-83 21.188893487589812 27.77181284229908 28.05459473279318 22.984698937317923 84-85 21.14839041788887 27.64261011580094 28.015963688455848 23.193035777854337 86-87 21.267838209762758 27.580114475252017 27.995559490700067 23.15648782428516 88-89 21.133021431566625 27.50293142374472 27.969322302982675 23.394724841705983 90-91 21.192607992130146 27.36917288394265 28.36752835123817 23.070690772689034 92-93 21.116568419795176 27.169002614301007 28.34730460512109 23.36712436078273 94-95 20.884971901664105 26.03783969288413 30.076421545651115 23.00076685980065 96-97 20.585025427171246 26.134401675502687 30.115057150843477 23.16551574648259 98-99 20.69638451944485 26.414432371435982 29.31449422528417 23.574688883834995 100-101 19.98773167191808 26.981945235242744 29.1304858453661 23.899837247473076 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 981.0 1 748.0 2 523.0 3 572.5 4 648.5 5 712.0 6 875.5 7 1310.5 8 1861.0 9 2195.0 10 2287.5 11 2413.5 12 2630.5 13 2780.0 14 2944.5 15 3102.5 16 3145.0 17 3332.0 18 3579.5 19 3762.5 20 4123.5 21 4634.0 22 5372.0 23 6255.0 24 7442.0 25 8887.0 26 10725.0 27 13312.5 28 15840.0 29 18508.0 30 22184.5 31 26026.5 32 29852.5 33 33614.0 34 36909.5 35 40728.5 36 44910.0 37 48712.0 38 51555.0 39 53613.5 40 56078.0 41 58655.5 42 61037.5 43 63367.0 44 64983.5 45 66567.0 46 67543.5 47 67720.0 48 67870.0 49 66745.5 50 64661.5 51 61878.5 52 58932.5 53 56085.5 54 52661.5 55 48362.5 56 43144.5 57 37910.0 58 33058.0 59 28142.0 60 23121.0 61 18455.5 62 14196.0 63 10522.0 64 7780.5 65 5752.5 66 4253.5 67 3183.0 68 2281.0 69 1677.5 70 1235.5 71 921.0 72 785.0 73 603.0 74 430.5 75 308.0 76 180.5 77 97.5 78 66.0 79 38.0 80 21.5 81 14.0 82 6.0 83 3.0 84 1.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021496623596987032 2 0.004184676060213476 3 0.0029235408091902366 4 0.0 5 0.05778292422870115 6 5.7324329591965416E-5 7 0.0 8 1.7197298877589626E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.0063515357187896E-4 16-17 0.0 18-19 9.745136030634121E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0016050812285750317 30-31 0.007366176352567557 32-33 2.0063515357187896E-4 34-35 4.2993247193974065E-4 36-37 0.025366015844444697 38-39 0.024563475230157183 40-41 0.026483840271488025 42-43 0.02694243490822375 44-45 0.02742969170975545 46-47 0.029120759432718434 48-49 0.028633502631186726 50-51 0.024334177911789323 52-53 0.021124015454639256 54-55 0.015620879813810579 56-57 0.14551781066920422 58-59 0.8023113169691479 60-61 0.31600036687570937 62-63 2.2929731836786167E-4 64-65 0.1568107035988214 66-67 1.4776779060568888 68-69 2.836522476869633 70-71 2.2834286828015546 72-73 1.9653646400605345 74-75 1.6418834481730735 76-77 1.1584673767240292 78-79 0.4529481902709148 80-81 2.2929731836786167E-4 82-83 3.439459775517925E-4 84-85 5.7324329591965416E-5 86-87 0.0037547435882737353 88-89 2.8662164795982708E-5 90-91 0.23778131914747258 92-93 0.187335909106543 94-95 1.3646916524311248 96-97 1.3564656111346778 98-99 1.0672357061784163 100-101 0.4280407690632059 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1744460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.24501545363379 #Duplication Level Percentage of deduplicated Percentage of total 1 76.5069764562111 39.97108167265546 2 12.798405498460767 13.373057860979088 3 4.325429042231576 6.779463214649553 4 1.9943794774434909 4.167855464777811 5 1.0956890654956275 2.8622146079598316 6 0.6503380715264495 2.0386153558191453 7 0.46770927119533373 1.7104834670985603 8 0.3132773719888684 1.3093744912665768 9 0.23814421877093447 1.1197663550892916 >10 1.42243768079096 13.947205096929412 >50 0.12415418382817028 4.47938000815297 >100 0.05898810170339941 5.331165179899904 >500 0.002861096464549924 0.984830244942819 >1k 8.803373737076689E-4 0.6403983711861098 >5k 3.3012651514037586E-4 1.2851086085935104 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9244 0.5299061027481283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7265 0.41646125448562876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5844 0.33500338213544595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2224 0.12748930901253108 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1828 0.1047888744941128 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.10117744172981898 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 5.732432959196542E-5 0.0 0.0 0.0 10-11 0.0 5.732432959196542E-5 0.0 0.0 0.0 12-13 0.0 5.732432959196542E-5 0.0 0.0 0.0 14-15 0.0 5.732432959196542E-5 0.0 0.0 0.0 16-17 0.0 5.732432959196542E-5 0.0 8.598649438794814E-5 0.0 18-19 0.0 5.732432959196542E-5 0.0 1.7197298877589626E-4 0.0 20-21 0.0 5.732432959196542E-5 0.0 3.7260814234777524E-4 0.0 22-23 0.0 5.732432959196542E-5 0.0 7.738784494915332E-4 0.0 24-25 0.0 5.732432959196542E-5 0.0 0.0015477568989830663 0.0 26-27 0.0 5.732432959196542E-5 0.0 0.0024362840076585304 0.0 28-29 0.0 5.732432959196542E-5 0.0 0.007050892539811747 0.0 30-31 0.0 5.732432959196542E-5 0.0 0.022614448024030356 0.0 32-33 0.0 5.732432959196542E-5 0.0 0.059473991951664126 0.0 34-35 0.0 5.732432959196542E-5 0.0 0.11659768639005766 0.0 36-37 0.0 5.732432959196542E-5 0.0 0.20843126239638626 0.0 38-39 0.0 5.732432959196542E-5 0.0 0.35973883035437904 0.0 40-41 0.0 5.732432959196542E-5 0.0 0.5248328995792394 0.0 42-43 0.0 5.732432959196542E-5 0.0 0.6881785767515449 0.0 44-45 0.0 5.732432959196542E-5 0.0 0.8867213922933171 0.0 46-47 0.0 5.732432959196542E-5 0.0 1.108939155956571 0.0 48-49 0.0 5.732432959196542E-5 0.0 1.3271728787131836 0.0 50-51 0.0 5.732432959196542E-5 0.0 1.5500785343315409 0.0 52-53 0.0 5.732432959196542E-5 0.0 1.7619205943386491 0.0 54-55 0.0 5.732432959196542E-5 0.0 1.9917911560024304 0.0 56-57 0.0 5.732432959196542E-5 0.0 2.186722538779909 0.0 58-59 0.0 5.732432959196542E-5 0.0 2.3099698474026344 0.0 60-61 0.0 1.1464865918393085E-4 0.0 2.398249314974261 0.0 62-63 5.732432959196542E-5 2.292973183678617E-4 0.0 2.475551173429027 0.0 64-65 5.732432959196542E-5 2.292973183678617E-4 0.0 2.554630086101143 0.0 66-67 5.732432959196542E-5 2.292973183678617E-4 0.0 2.622960686974766 2.866216479598271E-5 68-69 5.732432959196542E-5 2.292973183678617E-4 0.0 2.704303910665765 5.732432959196542E-5 70-71 5.732432959196542E-5 2.292973183678617E-4 0.0 2.8553248569757974 5.732432959196542E-5 72-73 5.732432959196542E-5 2.292973183678617E-4 0.0 3.0574504431170677 5.732432959196542E-5 74-75 5.732432959196542E-5 2.292973183678617E-4 0.0 3.2950024649461724 5.732432959196542E-5 76-77 5.732432959196542E-5 2.292973183678617E-4 0.0 3.649926051614826 5.732432959196542E-5 78-79 5.732432959196542E-5 2.292973183678617E-4 0.0 4.156873760361373 5.732432959196542E-5 80-81 5.732432959196542E-5 2.292973183678617E-4 0.0 4.713206379051398 5.732432959196542E-5 82-83 5.732432959196542E-5 2.292973183678617E-4 0.0 5.232937413296952 5.732432959196542E-5 84-85 5.732432959196542E-5 2.292973183678617E-4 0.0 5.642634396890728 5.732432959196542E-5 86-87 5.732432959196542E-5 2.292973183678617E-4 0.0 6.011287160496658 5.732432959196542E-5 88-89 5.732432959196542E-5 2.292973183678617E-4 0.0 6.3868475058184195 5.732432959196542E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4530 0.0 45.9866 1 GTATCAA 7640 0.0 38.309406 1 ATCAACG 9920 0.0 29.211815 3 TCAACGC 10040 0.0 28.955702 4 TATCAAC 10060 0.0 28.901453 2 CAACGCA 10120 0.0 28.587124 5 AACGCAG 10435 0.0 27.752607 6 CGCAGAG 12245 0.0 23.57411 8 ACGCAGA 12275 0.0 23.554182 7 GTACATG 8465 0.0 23.106192 1 TACATGG 8375 0.0 23.069147 2 TGGTATC 2440 0.0 22.01646 2 GCAGAGT 12995 0.0 21.923641 9 ACATGGG 8525 0.0 21.887487 3 TACGCGC 80 4.0408202E-5 21.359753 64-65 GTGGTAT 2800 0.0 21.040651 1 GAGTACT 7665 0.0 20.888468 12-13 GGACCGA 255 1.3182216E-8 20.312983 6 AGTACTT 7990 0.0 20.09775 12-13 AGAGTAC 11935 0.0 19.925194 10-11 >>END_MODULE