FastQCFastQC Report
Fri 27 May 2016
SRR939728_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939728_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892258
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92820.4905250763902174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64960.34329356779043874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48900.2584214203348592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20410.10786055601297498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41950.048.2146951
GTATCAA77600.042.2487641
ATCAACG98050.033.057653
TCAACGC98800.032.9495544
TATCAAC99700.032.839992
CAACGCA100500.032.1598555
AACGCAG103300.031.1995356
TACATGG90050.031.1874262
GTACATG92900.030.9869381
ACATGGG92200.029.2874373
ACGCAGA119450.026.9017757
CGCAGAG120150.026.5093948
CATGGGG37600.025.6487644
GCAGAGT126950.024.311189
CATGGGA58700.023.4015794
GGACCGA3100.022.7660376
GTAAGGT5900.022.325774
GAGTACT71950.021.80518212-13
AGTACTT75750.020.89763312-13
AGAGTAC116900.020.70453810-11
Produced by FastQC (version 0.11.5)