FastQCFastQC Report
Fri 27 May 2016
SRR939728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892258
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101980.5389328516513076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80910.42758439916755536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63100.3334640413727938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22860.12080805048782989No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.10738493376695989No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.044.0684741
GTATCAA87100.038.1324581
TATCAAC114000.029.0859972
ATCAACG114650.028.7510383
TCAACGC117350.028.1299464
CAACGCA119650.027.589215
AACGCAG122950.026.9258636
GTACCGT1701.1400334E-725.1094026
GTAAGGT6600.024.4330544
CGTACAC2351.8007995E-1024.2189963
GTACATG92000.023.2081031
ACGCAGA142550.023.0573167
CGCAGAG142500.023.0321248
TACATGG90700.022.855242
ACATGGG91200.022.1022683
GCAGAGT150300.021.3635039
GAGTACT84600.021.02343412-13
TAAGGTG8650.020.8358025
AGTACTT88500.020.28454812-13
CATGGGG37250.020.2448064
Produced by FastQC (version 0.11.5)