##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939728_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1892258 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.138408187467036 31.0 31.0 33.0 30.0 34.0 2 31.383129573240012 31.0 31.0 34.0 30.0 34.0 3 31.409317862574767 31.0 31.0 34.0 28.0 34.0 4 35.26516997153665 37.0 35.0 37.0 33.0 37.0 5 34.984809153931444 35.0 35.0 37.0 32.0 37.0 6 35.02957841901051 36.0 35.0 37.0 32.0 37.0 7 34.92608143287015 36.0 35.0 37.0 32.0 37.0 8 34.89172142487969 36.0 35.0 37.0 32.0 37.0 9 36.421921852094165 38.0 35.0 39.0 32.0 39.0 10-11 36.2733017907706 38.0 35.0 39.0 32.0 39.0 12-13 36.179597866675685 38.0 35.0 39.0 31.5 39.0 14-15 37.11050924345412 39.0 36.0 40.0 31.0 41.0 16-17 37.03492388458656 38.5 36.0 40.0 31.0 41.0 18-19 37.02285074234064 38.5 36.0 40.0 31.0 41.0 20-21 36.93778623211 39.0 36.0 40.0 31.0 41.0 22-23 36.78887894779676 39.0 35.5 40.0 30.5 41.0 24-25 36.60704592079938 38.0 35.0 40.0 30.0 41.0 26-27 36.268187266218455 38.0 35.0 40.0 30.0 41.0 28-29 36.00227453127428 38.0 34.0 40.0 29.0 41.0 30-31 35.682935149435224 38.0 34.0 40.0 28.0 41.0 32-33 35.6176203245012 38.0 34.0 40.0 28.5 41.0 34-35 35.4247245882961 38.0 34.0 40.0 27.0 41.0 36-37 35.42943483393913 38.0 34.0 40.0 27.0 41.0 38-39 35.2959221205565 38.0 34.0 40.0 27.0 41.0 40-41 35.18248832875855 38.0 34.0 40.0 27.0 41.0 42-43 34.907412995479476 38.0 33.0 40.0 26.0 41.0 44-45 34.607732138006554 38.0 33.0 40.0 25.0 41.0 46-47 34.26122283536389 38.0 33.0 40.0 24.0 41.0 48-49 33.898639614682565 37.0 33.0 40.0 23.5 41.0 50-51 33.63974098669421 37.0 32.5 40.0 23.0 41.0 52-53 33.362404069635325 37.0 32.0 40.0 22.0 41.0 54-55 33.052854050557585 37.0 31.0 39.5 20.5 41.0 56-57 32.65260154799187 36.0 31.0 39.0 19.5 40.0 58-59 32.239134409789784 36.0 31.0 39.0 18.0 40.0 60-61 31.897673573053993 35.0 30.0 39.0 17.0 40.0 62-63 31.576963870677254 35.0 30.0 38.5 13.5 40.0 64-65 31.102972480496845 35.0 29.0 38.0 11.0 40.0 66-67 30.09199353365133 34.0 28.0 37.5 7.5 40.0 68-69 28.023208780198047 33.0 23.0 36.5 6.5 39.0 70-71 27.326947752367808 32.0 21.5 36.0 4.0 39.0 72-73 27.89808392935847 32.0 25.0 36.0 2.0 38.5 74-75 28.029420670965585 32.0 26.0 35.0 2.0 38.0 76-77 27.75325219922442 31.5 26.0 35.0 2.0 37.0 78-79 28.31005100784354 33.0 26.0 35.0 2.0 37.0 80-81 28.108661186793768 33.0 26.0 35.0 2.0 37.0 82-83 27.622621228183473 32.5 26.0 35.0 2.0 36.0 84-85 27.211258454185423 32.0 26.0 35.0 2.0 36.0 86-87 26.761165232225203 32.0 25.0 34.5 2.0 35.5 88-89 26.234256110953158 32.0 24.5 34.0 2.0 35.0 90-91 25.662344141232325 31.0 23.0 34.0 2.0 35.0 92-93 25.06199656706432 31.0 20.0 34.0 2.0 35.0 94-95 24.125260138945112 31.0 16.0 34.0 2.0 35.0 96-97 23.48400165305154 30.0 4.5 34.0 2.0 35.0 98-99 22.69729154269661 30.0 2.0 34.0 2.0 35.0 100-101 21.16138417699912 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21089595895393032 1101 2 0.29100404141647473 1101 3 0.3467344389195901 1101 4 0.34610208910113727 1101 5 0.2635098385210597 1101 6 0.1813150443035454 1101 7 0.12059466952271691 1101 8 0.1275424289103242 1101 9 0.25814868794719814 1101 10-11 0.28128295161021555 1101 12-13 0.3574281804902384 1101 14-15 0.4440068338335408 1101 16-17 0.2803257602365008 1101 18-19 0.2822777616024297 1101 20-21 0.2559133759376735 1101 22-23 0.33537336792690553 1101 24-25 0.29319171630668706 1101 26-27 0.13420310245275147 1101 28-29 0.13794168959396558 1101 30-31 -0.05977837475887071 1101 32-33 -0.09728919708547323 1101 34-35 -0.04514401617759489 1101 36-37 0.21858946371947496 1101 38-39 0.2397018456355937 1101 40-41 0.27945134431531216 1101 42-43 0.10917863656420224 1101 44-45 0.07225042253694625 1101 46-47 0.05106192649297725 1101 48-49 0.24607266189833155 1101 50-51 0.18448134938753924 1101 52-53 -0.05389720191676162 1101 54-55 -0.16948938872793207 1101 56-57 -0.1805177275809271 1101 58-59 -0.15582459548716798 1101 60-61 -0.37526073559863704 1101 62-63 -0.10433700433918958 1101 64-65 -0.2909301771314645 1101 66-67 -0.757705189484799 1101 68-69 -9.159285933483655 1101 70-71 -7.100804636566263 1101 72-73 -2.226449626101296 1101 74-75 -1.2941190929792121 1101 76-77 -0.8464670695207133 1101 78-79 -1.114523377745133 1101 80-81 -0.8727619403421834 1101 82-83 -0.812933927032315 1101 84-85 -0.7058799435935263 1101 86-87 -0.7601684361454382 1101 88-89 -0.698382987314428 1101 90-91 -0.9523243628929343 1101 92-93 -1.1504276619969893 1101 94-95 -0.7142635826655805 1101 96-97 -1.0331163274240964 1101 98-99 -0.9313011137220748 1101 100-101 -0.73988881338515 1102 1 0.11979817554107441 1102 2 0.14314513892184166 1102 3 0.13115938303223373 1102 4 0.18716164111252453 1102 5 0.253493906453393 1102 6 0.181326166165789 1102 7 0.15139944747473777 1102 8 -0.023539033169690526 1102 9 0.2143125630264393 1102 10-11 0.19788740535995686 1102 12-13 0.11922187272606521 1102 14-15 0.15407830570079284 1102 16-17 0.2448866413104298 1102 18-19 0.11240348035277492 1102 20-21 0.18678196557432614 1102 22-23 0.14458129670249775 1102 24-25 0.009664862570296862 1102 26-27 0.0850708857543836 1102 28-29 0.08037666840682078 1102 30-31 -0.10089555191900956 1102 32-33 -0.1111849212855347 1102 34-35 -0.0941170512225753 1102 36-37 0.019142150761389587 1102 38-39 0.16589438731477202 1102 40-41 0.015831581322196087 1102 42-43 -0.11537454262012403 1102 44-45 -0.010642554563141005 1102 46-47 -0.10698772984147809 1102 48-49 -0.21226136655139527 1102 50-51 -0.37035777287508864 1102 52-53 -0.3271425891243993 1102 54-55 -0.35017342496296777 1102 56-57 -0.6924198579736363 1102 58-59 -0.5724610245908615 1102 60-61 -0.5533568535126214 1102 62-63 -0.41505125033253876 1102 64-65 -0.5661417010490304 1102 66-67 0.010425878881289208 1102 68-69 -5.034598735859287 1102 70-71 -0.5649373259437702 1102 72-73 -0.20027814644874198 1102 74-75 -0.15483340848419758 1102 76-77 -0.41408799196796764 1102 78-79 -0.3552585189371733 1102 80-81 -0.45248136356240565 1102 82-83 -0.5820517131144207 1102 84-85 -0.5676345007765811 1102 86-87 -0.6074520603154703 1102 88-89 -0.6559016442383445 1102 90-91 -0.7666462629518769 1102 92-93 -0.8474698722446767 1102 94-95 -0.6427524413400754 1102 96-97 -0.8761117785824375 1102 98-99 -0.6565313362705361 1102 100-101 -0.7106775897578679 1103 1 0.17928538929853843 1103 2 0.28907871283255915 1103 3 0.23956399855392974 1103 4 0.2789430275510867 1103 5 0.3352066837856569 1103 6 0.22468578480608414 1103 7 0.2135374676119568 1103 8 0.2113636220918309 1103 9 0.44490487346037355 1103 10-11 0.37328335564063053 1103 12-13 0.41922799853912096 1103 14-15 0.3690859908117261 1103 16-17 0.42511363052899753 1103 18-19 0.4450267762629636 1103 20-21 0.4055681635606021 1103 22-23 0.24954988798825184 1103 24-25 0.37824389669665237 1103 26-27 0.42712514932027545 1103 28-29 0.4247225429501711 1103 30-31 0.17359934694938062 1103 32-33 0.32391765823865626 1103 34-35 0.027675250301079757 1103 36-37 0.32649925224940546 1103 38-39 0.47582154582853775 1103 40-41 0.45654206425901833 1103 42-43 0.30814746575553187 1103 44-45 0.41534370133991416 1103 46-47 0.3639230114932843 1103 48-49 0.20932822624910585 1103 50-51 0.41310587320884196 1103 52-53 0.2501428960399963 1103 54-55 0.23667111303935684 1103 56-57 0.3964796118650824 1103 58-59 0.0636072058288093 1103 60-61 0.05696625528181798 1103 62-63 0.010748446718160665 1103 64-65 -0.023291638677115145 1103 66-67 0.16374662645323923 1103 68-69 -4.299167033106013 1103 70-71 0.2860105044046186 1103 72-73 0.5952839804335532 1103 74-75 0.43637716355536327 1103 76-77 0.21853029191334628 1103 78-79 0.15080882045514343 1103 80-81 0.16131953637522045 1103 82-83 0.09551333188246147 1103 84-85 0.06374748265031016 1103 86-87 0.08921995251532522 1103 88-89 -0.2064618776875129 1103 90-91 -0.27337908839961855 1103 92-93 -0.021234128682348086 1103 94-95 -0.3280833466238704 1103 96-97 -0.8976300891945748 1103 98-99 -0.47974594020887906 1103 100-101 -0.37195075646908293 1104 1 0.24637970596724657 1104 2 0.22154580455291395 1104 3 0.30247871334884024 1104 4 0.21433329063187045 1104 5 0.2503725882487231 1104 6 0.22422447544785484 1104 7 0.24089380799613735 1104 8 0.2273272440240035 1104 9 0.31924466790648864 1104 10-11 0.2809475354094033 1104 12-13 0.1913182260133226 1104 14-15 0.30487033726386414 1104 16-17 0.41939107634948414 1104 18-19 0.28994129262530777 1104 20-21 0.37836899289747095 1104 22-23 0.3963630980337598 1104 24-25 0.34477671410530064 1104 26-27 0.41244299378623595 1104 28-29 0.5345955368339972 1104 30-31 0.3973557125548979 1104 32-33 0.29614382129459926 1104 34-35 0.4271318325133606 1104 36-37 0.5446447979018743 1104 38-39 0.5994063228978916 1104 40-41 0.44694659779412405 1104 42-43 0.4981223093276199 1104 44-45 0.26496890487239 1104 46-47 0.31910785050327206 1104 48-49 0.19225537553345617 1104 50-51 0.1468401979632148 1104 52-53 -0.054882501474217804 1104 54-55 -0.008860445157019115 1104 56-57 0.10316428582368786 1104 58-59 0.2892125178872078 1104 60-61 0.0644373805613867 1104 62-63 0.28860596842519826 1104 64-65 0.37044957558184066 1104 66-67 0.4691881296448521 1104 68-69 -4.666109650858122 1104 70-71 0.522985353540232 1104 72-73 0.9683703579450231 1104 74-75 0.8092053005517208 1104 76-77 0.6792448160646316 1104 78-79 0.3638310504663096 1104 80-81 0.3973337493397331 1104 82-83 0.5036112351734765 1104 84-85 0.38819099056721384 1104 86-87 0.28614874489551667 1104 88-89 0.19614458973060067 1104 90-91 0.22473974303655098 1104 92-93 0.06156046950097149 1104 94-95 0.20209787609165275 1104 96-97 0.13417661152089622 1104 98-99 0.5019321966020307 1104 100-101 0.4783858834555481 1105 1 0.16506881337597434 1105 2 0.2791808839692145 1105 3 0.2546123954081345 1105 4 0.20040736848003604 1105 5 0.24603379946758253 1105 6 0.2535642549277455 1105 7 0.16388868089420328 1105 8 0.18126682838342134 1105 9 0.2885141360805221 1105 10-11 0.24437211681559035 1105 12-13 0.2677567677802344 1105 14-15 0.27702273881199346 1105 16-17 0.25455673061637896 1105 18-19 0.176604883408217 1105 20-21 0.34788684340197307 1105 22-23 0.3377649179001949 1105 24-25 0.25525623117268736 1105 26-27 0.23657934035341555 1105 28-29 0.4490428024110784 1105 30-31 0.49324544495301126 1105 32-33 0.437671271198262 1105 34-35 0.5136309201269853 1105 36-37 0.3843318125774289 1105 38-39 0.7010727725543617 1105 40-41 0.48020694965258315 1105 42-43 0.32034330497042873 1105 44-45 0.04394825871035124 1105 46-47 0.0707019319745541 1105 48-49 0.06094061449942245 1105 50-51 -0.003002045054252278 1105 52-53 0.011826199125295034 1105 54-55 0.14426336954267782 1105 56-57 0.11846657160159424 1105 58-59 0.19785806285644014 1105 60-61 0.3071805121884488 1105 62-63 0.3990155704662257 1105 64-65 0.2301616785239311 1105 66-67 0.7592968045805506 1105 68-69 1.995961352993337 1105 70-71 2.392231516705486 1105 72-73 1.5089972626813193 1105 74-75 0.7860906474566853 1105 76-77 0.645876194985199 1105 78-79 0.5680678444519209 1105 80-81 0.6238791922198139 1105 82-83 0.6192748429397987 1105 84-85 0.387817355608572 1105 86-87 0.24874892340999466 1105 88-89 0.5037157454892025 1105 90-91 0.49347998937143345 1105 92-93 0.42234488590392516 1105 94-95 0.6702278048179515 1105 96-97 0.2688517283761733 1105 98-99 0.43127862791641647 1105 100-101 0.5494925788807841 1106 1 0.3612596915476942 1106 2 0.26283189900098236 1106 3 0.340429273726663 1106 4 0.26802789585612885 1106 5 0.209611531570971 1106 6 0.32582358759227503 1106 7 0.3150780501798067 1106 8 0.36138806942185653 1106 9 0.3842815066221732 1106 10-11 0.3983218301272373 1106 12-13 0.4415121406382312 1106 14-15 0.46042682666995205 1106 16-17 0.5558236851948237 1106 18-19 0.49144263737006355 1106 20-21 0.5226979267822003 1106 22-23 0.41509838988792325 1106 24-25 0.47884766559322856 1106 26-27 0.415531704410931 1106 28-29 0.5324531093266742 1106 30-31 0.4213740387527096 1106 32-33 0.6196572100108924 1106 34-35 0.7725593459101745 1106 36-37 0.8186590736636603 1106 38-39 0.9735250999092315 1106 40-41 0.8976540570771192 1106 42-43 0.7317375361317815 1106 44-45 0.8968318624197664 1106 46-47 1.0311798206758596 1106 48-49 0.9479892740376243 1106 50-51 0.8713490319641934 1106 52-53 0.7462413426326009 1106 54-55 0.8391054258689863 1106 56-57 0.9747140737833604 1106 58-59 1.12338994521226 1106 60-61 1.1950325516315523 1106 62-63 1.1793829230643667 1106 64-65 1.0427412009003056 1106 66-67 1.6286196205698502 1106 68-69 2.8753478276644415 1106 70-71 3.3222135935852712 1106 72-73 2.1203214529804058 1106 74-75 1.643805745289601 1106 76-77 1.1872487308110529 1106 78-79 1.0850825744428647 1106 80-81 1.1507771262692579 1106 82-83 1.290357550289297 1106 84-85 1.3644286012960087 1106 86-87 1.3025249109147872 1106 88-89 1.619259719324301 1106 90-91 1.401520517236893 1106 92-93 1.3777873769525577 1106 94-95 1.7330229551764127 1106 96-97 1.426481390630368 1106 98-99 1.930388079252797 1106 100-101 1.948061787816652 1107 1 0.08538086157585312 1107 2 0.04727895176455732 1107 3 0.06820519847196493 1107 4 -0.08963035898134564 1107 5 0.005963321107763875 1107 6 0.014956748382481067 1107 7 -0.04219757690749759 1107 8 0.03252611518097126 1107 9 -0.11332561859262569 1107 10-11 -0.018657183928134202 1107 12-13 -0.02810088284930856 1107 14-15 0.047120775135006454 1107 16-17 0.03546551395492514 1107 18-19 -0.006929479463870791 1107 20-21 0.03639309754500886 1107 22-23 0.04900649167429805 1107 24-25 0.049783683900251674 1107 26-27 -0.0629522136127818 1107 28-29 0.00895211291123843 1107 30-31 -9.31681347431379E-4 1107 32-33 0.15536983704330254 1107 34-35 -0.0047668724821292585 1107 36-37 -0.08768288893698184 1107 38-39 0.06596400613219089 1107 40-41 -0.01874389056040826 1107 42-43 0.002251925046238057 1107 44-45 -0.038111920327885684 1107 46-47 -0.061504495069613085 1107 48-49 -0.21049302903310974 1107 50-51 -0.10737660124515713 1107 52-53 -0.2007868799902539 1107 54-55 -0.15933450843514407 1107 56-57 -0.1612647038344548 1107 58-59 -0.07595198783369028 1107 60-61 -0.052790956172994896 1107 62-63 0.039545938318809704 1107 64-65 0.06407995470547334 1107 66-67 0.6976533192555117 1107 68-69 1.9367754144391363 1107 70-71 2.1063146980864538 1107 72-73 1.1392575683957098 1107 74-75 0.6000289085708452 1107 76-77 0.18866234243702706 1107 78-79 0.25967535296419797 1107 80-81 0.32050604638291347 1107 82-83 0.32200192075318057 1107 84-85 0.1139224771409566 1107 86-87 -0.00470221480666666 1107 88-89 0.054780346390490564 1107 90-91 0.05053687364963011 1107 92-93 0.09257849914433436 1107 94-95 0.49201497118661663 1107 96-97 0.09765796304570173 1107 98-99 0.009557455099859169 1107 100-101 0.37342404393764284 1108 1 -0.1749788366131959 1108 2 -0.1971565645904576 1108 3 -0.12230984847317572 1108 4 -0.02641740951433036 1108 5 0.04408989650028872 1108 6 0.047234387048170845 1108 7 -0.11903312980538772 1108 8 -0.022707613895114775 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0.1588814206860576 2316 76-77 -0.34821087454046307 2316 78-79 -0.4749308560029242 2316 80-81 -0.48976603195172785 2316 82-83 -0.17154711937338973 2316 84-85 -0.41255626362469755 2316 86-87 -0.5597955860117061 2316 88-89 -0.48751232468623584 2316 90-91 -0.5420625707325293 2316 92-93 -0.39721326874194673 2316 94-95 -0.1799671269559262 2316 96-97 -0.34295788825800244 2316 98-99 -0.5198747184123711 2316 100-101 -0.5408258096832164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 18.0 4 50.0 5 130.0 6 320.0 7 767.0 8 1618.0 9 2750.0 10 4365.0 11 6411.0 12 8847.0 13 11283.0 14 13668.0 15 17282.0 16 20644.0 17 23819.0 18 21886.0 19 19086.0 20 18595.0 21 19378.0 22 21044.0 23 23862.0 24 28304.0 25 33622.0 26 41137.0 27 50616.0 28 60630.0 29 72441.0 30 85534.0 31 100215.0 32 117394.0 33 140458.0 34 170100.0 35 204609.0 36 234749.0 37 218037.0 38 94093.0 39 4494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.4961782833462 20.945174488059116 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27.323758176739112 30.08788970637196 21.046997819536237 20-21 22.141827382946722 26.56355528685835 30.231923976540198 21.062693353654733 22-23 21.841921133376104 26.65553534454604 30.125226052684145 21.37731746939371 24-25 21.67678597568935 26.866170927099226 30.151031281691655 21.306011815519774 26-27 21.39255236617533 27.16625481172698 30.070096371942 21.371096450155687 28-29 21.33059691711904 27.24377674056787 30.224372389359576 21.20125395295351 30-31 21.421682430079308 26.96574080288503 30.14837883557731 21.464197931458354 32-33 21.212917934576385 27.109002009277983 29.945455660065257 21.732624396080375 34-35 21.786128016877814 27.016845049223438 29.815457026468827 21.381569907429917 36-37 21.799982860026727 26.733597204279913 29.829742932141578 21.63667700355178 38-39 21.38818308841829 27.057573305904754 29.99147573467239 21.562767871004564 40-41 21.569186551386387 26.97531042169951 29.973710403924475 21.48179262298963 42-43 21.545684792456075 26.995800847765857 29.70346711104939 21.755047248728683 44-45 21.504324902354778 27.10229823007375 29.64989490261134 21.743481964960132 46-47 21.717881269012736 27.373633238455884 29.1547144181805 21.753771074350876 48-49 21.597404256433357 27.40857544342586 28.999335882774712 21.99468441736607 50-51 21.540633530309286 27.58244307132261 28.919337011898694 21.957586386469412 52-53 21.998684741815136 27.490482623598965 28.671015427444367 21.83981720714153 54-55 21.488882243714162 27.473875606030678 28.935778792885504 22.101463357369653 56-57 21.58623506073506 27.681706264612167 28.71534546492618 22.016713209726596 58-59 21.58380993583077 27.31271246261464 28.74396026745943 22.35951733409516 60-61 21.38859768435801 27.443499640997004 29.07348158382026 22.094421090824717 62-63 21.321763468148035 27.50909053915399 28.989313843647217 22.179832149050753 64-65 21.095479192871565 27.428387962835732 28.918314712441994 22.55781813185071 66-67 21.061681091230756 27.585794349777636 28.859971530256644 22.49255302873496 68-69 21.351531987881483 27.02265028568334 29.296517705041435 22.329300021393735 70-71 21.298843153380297 27.22210589977711 29.058045607775046 22.42100533906754 72-73 21.162613981762917 27.347627167109707 28.76581976848861 22.723939082638765 74-75 21.189838989132458 27.690036568749115 28.23836956507374 22.881754877044685 76-77 21.157655032228305 27.58597629233724 28.416550692343577 22.839817983090878 78-79 21.22897646600891 27.64358049410309 28.26401703260681 22.86342600728119 80-81 21.23040498672857 27.81861012645183 28.146267154100535 22.804717732719066 82-83 21.2321846345396 27.7646218246253 28.0291736270496 22.974019913785497 84-85 21.14144129099357 27.615619094325904 28.02061357707869 23.22232603760184 86-87 21.30716311859964 27.539156893132567 28.023903045484754 23.129776942783042 88-89 21.09564072678423 27.53455073540622 27.927442068412084 23.442366469397466 90-91 21.0409648436024 27.59379139429392 28.04677196842163 23.318471793682054 92-93 21.23242949005059 27.300453573160304 28.047362920625357 23.419754016163747 94-95 20.84927718136833 27.02997615331906 28.307731936542492 23.81301472877012 96-97 21.137439580905756 26.855558953099536 28.180266342193878 23.826735123800834 98-99 21.056317741053093 27.01615610075825 27.893416778770042 24.034109379418613 100-101 20.720543352763077 27.025542135816522 28.265083464563602 23.988831046856802 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1698.0 1 1160.5 2 771.5 3 979.5 4 876.0 5 808.0 6 1411.5 7 2853.5 8 4115.5 9 4396.0 10 3705.5 11 2850.0 12 2701.0 13 2814.5 14 2884.5 15 2968.0 16 3038.0 17 3118.0 18 3391.0 19 3718.0 20 4122.0 21 4786.5 22 5655.5 23 6665.5 24 7793.0 25 9529.5 26 12021.0 27 14880.0 28 17255.5 29 20137.0 30 24114.0 31 27843.0 32 32472.0 33 36176.5 34 39072.0 35 42571.0 36 46858.0 37 51781.5 38 54512.5 39 55576.5 40 56958.5 41 59843.5 42 62994.0 43 65573.5 44 67836.0 45 69891.5 46 70283.0 47 70364.0 48 71720.0 49 70953.0 50 68592.0 51 65740.5 52 62851.5 53 60823.0 54 58676.5 55 54271.5 56 48152.5 57 43216.0 58 39550.5 59 35145.0 60 29453.0 61 23780.5 62 18541.5 63 13568.0 64 9829.0 65 7450.0 66 5677.0 67 4454.5 68 3257.0 69 2254.0 70 1711.5 71 1358.5 72 1121.0 73 938.5 74 741.5 75 548.0 76 358.5 77 230.5 78 135.5 79 67.5 80 39.0 81 28.5 82 20.5 83 11.5 84 8.0 85 3.5 86 2.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023146949306067144 2 0.01865496142703585 3 5.284691622389759E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.227753297911807E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.284691622389759E-5 26-27 3.1708149734338553E-4 28-29 1.0569383244779518E-4 30-31 3.6992841356728307E-4 32-33 0.0019024889840603132 34-35 0.003699284135672831 36-37 0.10252301747436132 38-39 0.09367115900685848 40-41 0.10392346075429461 42-43 0.10545602132478764 44-45 0.09874446296435264 46-47 0.09242925647559688 48-49 0.09398824050420185 50-51 0.0928256083472761 52-53 0.07263808634974724 54-55 0.06138169319405705 56-57 0.033848449841406406 58-59 0.01625042673884851 60-61 0.011229969697578238 62-63 0.0022988408557395447 64-65 0.0062359361144199155 66-67 0.1709597739843087 68-69 0.4507313484736225 70-71 0.4295133116097276 72-73 0.20071258781836301 74-75 0.032527276935808966 76-77 0.03569809190924282 78-79 0.006473747237427455 80-81 0.0018760655259483642 82-83 5.284691622389759E-5 84-85 5.284691622389759E-4 86-87 0.0016118309448288765 88-89 2.3781112300753915E-4 90-91 7.134333690226174E-4 92-93 0.003197238431545804 94-95 0.0865896722328562 96-97 0.01947408862850626 98-99 0.021297307238230728 100-101 0.0120226734409367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1892258.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.4156453099028 #Duplication Level Percentage of deduplicated Percentage of total 1 76.04889455851584 39.10102988830849 2 12.952269996030848 13.318986401480368 3 4.434671527092284 6.840344949087057 4 2.043356997523086 4.202420745046197 5 1.1313775449629835 2.9085253281702688 6 0.6964552698298386 2.148521827667018 7 0.46874359395525256 1.6870528057664658 8 0.3271847657177765 1.3457932691959076 9 0.23735857163214047 1.0983549717272962 >10 1.4639945037997488 14.131827169736505 >50 0.1289838037151291 4.5792646057590325 >100 0.06248876243944427 5.619218137176758 >500 0.002984952165431223 1.0358914603751384 >1k 9.263644651338278E-4 0.680944897345811 >5k 2.0585877002973952E-4 0.7621269500798185 >10k+ 1.0292938501486976E-4 0.5396965930778409 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10198 0.5389328516513076 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8091 0.42758439916755536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6310 0.3334640413727938 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2286 0.12080805048782989 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2032 0.10738493376695989 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.3211729055974397E-4 0.0 18-19 0.0 0.0 0.0 2.1138766489559035E-4 0.0 20-21 0.0 0.0 0.0 2.9065803923143674E-4 0.0 22-23 0.0 0.0 0.0 6.605864527987199E-4 0.0 24-25 0.0 0.0 0.0 0.0011890556150376957 0.0 26-27 0.0 0.0 0.0 0.0026687692693068282 0.0 28-29 0.0 0.0 0.0 0.0071343336902261745 0.0 30-31 0.0 0.0 0.0 0.02322621968040299 0.0 32-33 0.0 0.0 0.0 0.05739175101915278 0.0 34-35 0.0 0.0 0.0 0.1138322575462754 0.0 36-37 0.0 0.0 0.0 0.20856035487761182 0.0 38-39 0.0 0.0 0.0 0.3520133089673818 0.0 40-41 0.0 0.0 0.0 0.5143590356071952 0.0 42-43 0.0 0.0 0.0 0.6766783387888966 0.0 44-45 0.0 0.0 0.0 0.8713663781577354 0.0 46-47 0.0 0.0 0.0 1.1070372010582066 0.0 48-49 0.0 0.0 0.0 1.3563953752606674 0.0 50-51 0.0 0.0 0.0 1.6308822581275915 0.0 52-53 0.0 0.0 0.0 1.891655366234414 0.0 54-55 0.0 0.0 0.0 2.168678901080085 0.0 56-57 0.0 0.0 0.0 2.4807399413822004 0.0 58-59 0.0 0.0 0.0 2.800252396871885 0.0 60-61 0.0 0.0 0.0 3.125155237816408 0.0 62-63 0.0 0.0 0.0 3.4414176079583227 2.6423458111948794E-5 64-65 0.0 0.0 0.0 3.7603751708276567 5.284691622389759E-5 66-67 0.0 0.0 0.0 4.106548895552298 5.284691622389759E-5 68-69 0.0 0.0 0.0 4.5022930276949555 5.284691622389759E-5 70-71 0.0 0.0 0.0 4.9472376388420605 5.284691622389759E-5 72-73 0.0 0.0 0.0 5.407534279152209 5.284691622389759E-5 74-75 0.0 0.0 0.0 5.871239545558798 5.284691622389759E-5 76-77 0.0 0.0 0.0 6.369453848259592 5.284691622389759E-5 78-79 0.0 0.0 0.0 6.915045411355111 5.284691622389759E-5 80-81 0.0 0.0 0.0 7.505741817447726 5.284691622389759E-5 82-83 0.0 0.0 0.0 8.092448281365439 5.284691622389759E-5 84-85 0.0 0.0 0.0 8.66060547768856 5.284691622389759E-5 86-87 0.0 0.0 0.0 9.22466703800433 5.284691622389759E-5 88-89 0.0 0.0 0.0 9.830451238678869 5.284691622389759E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5125 0.0 44.068474 1 GTATCAA 8710 0.0 38.132458 1 TATCAAC 11400 0.0 29.085997 2 ATCAACG 11465 0.0 28.751038 3 TCAACGC 11735 0.0 28.129946 4 CAACGCA 11965 0.0 27.58921 5 AACGCAG 12295 0.0 26.925863 6 GTACCGT 170 1.1400334E-7 25.109402 6 GTAAGGT 660 0.0 24.433054 4 CGTACAC 235 1.8007995E-10 24.218996 3 GTACATG 9200 0.0 23.208103 1 ACGCAGA 14255 0.0 23.057316 7 CGCAGAG 14250 0.0 23.032124 8 TACATGG 9070 0.0 22.85524 2 ACATGGG 9120 0.0 22.102268 3 GCAGAGT 15030 0.0 21.363503 9 GAGTACT 8460 0.0 21.023434 12-13 TAAGGTG 865 0.0 20.835802 5 AGTACTT 8850 0.0 20.284548 12-13 CATGGGG 3725 0.0 20.244806 4 >>END_MODULE