FastQCFastQC Report
Fri 27 May 2016
SRR939727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513215
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88400.25162137813939656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61720.175679541388728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.11943476274580406No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106250.037.815461
GGTATCA62750.037.6470151
TCAACGC131650.031.3144534
ATCAACG131800.031.242823
TATCAAC132550.031.172082
CAACGCA134500.030.5098255
AACGCAG138050.029.8627136
ACGCAGA155150.026.2655987
CGCAGAG156900.025.9424088
TACATGG108400.025.3820112
GTACATG106750.025.2847881
ACATGGG111750.023.6882293
GTATAGG9700.023.4738541
GCAGAGT168300.023.0291399
AGAGTAC141300.021.09933510-11
CATGGGG65000.020.5087364
GAGTACT97500.020.4817812-13
AGTACTT103200.019.6722612-13
GTACTTT103600.019.20931814-15
CAGAGTA162850.018.65679410-11
Produced by FastQC (version 0.11.5)