##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939727_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3513215 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.2739729848586 31.0 30.0 33.0 23.0 34.0 2 30.180690051704776 31.0 30.0 33.0 25.0 34.0 3 30.485814560167825 31.0 30.0 34.0 26.0 34.0 4 34.28498483582701 35.0 35.0 37.0 30.0 37.0 5 34.257165303005934 35.0 35.0 37.0 30.0 37.0 6 34.2961082655061 37.0 35.0 37.0 30.0 37.0 7 34.22197759032681 36.0 35.0 37.0 30.0 37.0 8 34.09773868095178 36.0 35.0 37.0 30.0 37.0 9 35.43811238424065 38.0 35.0 39.0 30.0 39.0 10-11 35.29671326690794 38.0 35.0 39.0 29.5 39.0 12-13 35.192978795775375 37.0 35.0 39.0 29.0 39.0 14-15 35.954465496703165 38.0 35.0 40.0 27.0 41.0 16-17 35.79108081913574 38.0 34.5 40.0 27.0 41.0 18-19 35.62876780954197 38.0 34.0 40.0 27.0 41.0 20-21 35.81601766473159 38.0 34.0 40.0 27.0 41.0 22-23 35.725996701027405 38.0 34.0 40.0 27.0 41.0 24-25 35.53311368646667 38.0 34.0 40.0 27.0 41.0 26-27 35.4423143758637 38.0 34.0 40.0 27.0 41.0 28-29 35.5167227169416 38.0 34.0 40.0 27.0 41.0 30-31 35.32413430433378 38.0 34.0 40.0 26.0 41.0 32-33 35.008612339409915 38.0 34.0 40.0 25.0 41.0 34-35 34.84469567048985 38.0 34.0 40.0 25.0 41.0 36-37 34.65430481766701 38.0 33.5 40.0 24.0 41.0 38-39 34.44615060564184 38.0 33.0 40.0 24.0 41.0 40-41 34.236811012135604 38.0 33.0 40.0 24.0 41.0 42-43 33.873243453645735 38.0 33.0 40.0 22.5 41.0 44-45 33.557094000794145 38.0 32.5 40.0 20.0 41.0 46-47 33.209334185354436 37.5 31.5 40.0 18.5 41.0 48-49 32.87509517635556 37.0 31.0 40.0 16.0 41.0 50-51 32.25701686347121 36.5 30.5 39.5 15.0 40.5 52-53 32.42483949886358 37.0 31.0 39.0 15.0 40.0 54-55 32.54696552872511 37.0 31.0 39.5 13.5 40.5 56-57 32.26389176295786 36.5 31.0 39.0 10.5 41.0 58-59 31.875587745128037 36.0 30.5 39.0 8.5 40.0 60-61 31.485527928122814 36.0 30.0 39.0 8.0 40.0 62-63 31.055813834336924 35.0 29.0 39.0 6.5 40.0 64-65 30.526119380681227 35.0 28.5 38.0 2.0 40.0 66-67 29.941395701657882 34.0 28.0 38.0 2.0 40.0 68-69 29.38850241160874 34.0 27.5 37.5 2.0 39.5 70-71 28.811378609051822 34.0 26.0 37.0 2.0 39.0 72-73 28.151499410084497 33.0 26.0 36.0 2.0 39.0 74-75 27.799822669549116 33.0 26.0 36.0 2.0 38.5 76-77 27.234490488057233 32.5 25.0 35.0 2.0 38.0 78-79 26.569794049040553 32.0 23.5 35.0 2.0 37.0 80-81 25.837596332703804 31.5 21.0 35.0 2.0 36.5 82-83 25.090574872303577 31.0 19.0 34.0 2.0 36.0 84-85 24.303522841613734 31.0 15.0 34.0 2.0 35.5 86-87 23.37470778190347 30.0 4.5 34.0 2.0 35.0 88-89 22.366228938450963 29.5 2.0 34.0 2.0 35.0 90-91 20.910125341033783 28.0 2.0 33.5 2.0 35.0 92-93 19.295657111790767 25.5 2.0 32.5 2.0 34.0 94-95 18.79584853190027 25.0 2.0 32.0 2.0 34.0 96-97 18.51165812510763 25.0 2.0 32.0 2.0 34.0 98-99 17.59901856276943 24.0 2.0 32.0 2.0 34.0 100-101 15.883353851102196 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5948719340378048 1101 2 0.8490269134224917 1101 3 0.8852758753784578 1101 4 0.7845403021908908 1101 5 0.8071626828283343 1101 6 0.7683741742251087 1101 7 0.7427807343546107 1101 8 0.8489435516039023 1101 9 0.8892053313779229 1101 10-11 0.9480834581340787 1101 12-13 0.8558081404669977 1101 14-15 1.1440885902945368 1101 16-17 1.1045879353743473 1101 18-19 1.1032740484670143 1101 20-21 1.0312728314528172 1101 22-23 1.1721737184192662 1101 24-25 1.2435765707095356 1101 26-27 1.0845130484956655 1101 28-29 1.0860349558818285 1101 30-31 1.1114722980191942 1101 32-33 1.2841446774742167 1101 34-35 1.1483266608170055 1101 36-37 1.11681305803166 1101 38-39 1.1510067399756494 1101 40-41 1.0991156360820966 1101 42-43 1.1904711895135236 1101 44-45 1.0868397919758834 1101 46-47 0.8525318581968833 1101 48-49 0.9696775236362001 1101 50-51 0.8499174399890634 1101 52-53 0.8669279639071803 1101 54-55 0.8880535627572854 1101 56-57 1.0670643281768761 1101 58-59 0.9654646948624865 1101 60-61 0.9134077150707505 1101 62-63 0.33528784889189467 1101 64-65 0.5725285404492162 1101 66-67 0.8754082359644322 1101 68-69 0.598678889461354 1101 70-71 0.6330269493631491 1101 72-73 0.6184560579689666 1101 74-75 0.47160544627012513 1101 76-77 0.4232851308938592 1101 78-79 0.16100293664831256 1101 80-81 -0.009330033312505037 1101 82-83 -0.18294131872549713 1101 84-85 -0.1685168756994564 1101 86-87 -0.6961625044756943 1101 88-89 -4.721285510644915 1101 90-91 -8.458120678804807 1101 92-93 -11.14456733063235 1101 94-95 -9.105989102319741 1101 96-97 -5.4095684772282375 1101 98-99 -3.7569668191197323 1101 100-101 -2.7365527927814686 1102 1 1.892733195580572 1102 2 1.2009095900542839 1102 3 1.0330462118104506 1102 4 1.043149574917173 1102 5 1.035701495406343 1102 6 1.0245375069710505 1102 7 1.065569192224764 1102 8 1.2137360497826748 1102 9 1.3779534624626848 1102 10-11 1.339814791236094 1102 12-13 1.3230481909345215 1102 14-15 1.6296019992504327 1102 16-17 1.599825494257665 1102 18-19 1.5976812685203186 1102 20-21 1.5811408492490742 1102 22-23 1.4000796431757863 1102 24-25 1.5453330331141402 1102 26-27 1.589075086489828 1102 28-29 1.3684464424210958 1102 30-31 1.5220568529221623 1102 32-33 1.7773549288489932 1102 34-35 1.7355939888177687 1102 36-37 1.644669092061811 1102 38-39 1.576600637183951 1102 40-41 1.6386537528695442 1102 42-43 1.5282052921526272 1102 44-45 1.7250309726943058 1102 46-47 1.7998947574816526 1102 48-49 1.7646006369628608 1102 50-51 1.7342989176084131 1102 52-53 1.8745321553640295 1102 54-55 1.839739378803344 1102 56-57 1.8330433263838088 1102 58-59 1.6199389415284209 1102 60-61 1.6761460869208484 1102 62-63 1.680427102379472 1102 64-65 1.5629774298185204 1102 66-67 1.6159908788058708 1102 68-69 1.4486423513736355 1102 70-71 1.483384177864039 1102 72-73 1.5238848593380219 1102 74-75 1.462303675728407 1102 76-77 1.3500858750082543 1102 78-79 1.2204075315910252 1102 80-81 1.2016774232732494 1102 82-83 1.001997041134473 1102 84-85 1.0559122535813366 1102 86-87 1.0067268657339312 1102 88-89 0.7246317611294266 1102 90-91 -5.575850643942612 1102 92-93 -11.046992015391995 1102 94-95 -8.519932935853213 1102 96-97 -4.603078300046896 1102 98-99 -2.8801343511342736 1102 100-101 -1.965711095125176 1103 1 2.0444323182658657 1103 2 1.21391930116231 1103 3 1.0070142620337634 1103 4 0.8845561180299697 1103 5 0.9157634329509889 1103 6 0.7243900029750776 1103 7 0.7223726534291544 1103 8 0.8910548370120139 1103 9 1.0912220122465683 1103 10-11 0.9447391344064471 1103 12-13 0.8570649348223611 1103 14-15 1.050757387257896 1103 16-17 0.9023892916632477 1103 18-19 0.9097587845684387 1103 20-21 0.8410966462332041 1103 22-23 0.8916007331635072 1103 24-25 0.8516766204164554 1103 26-27 0.9009690255917491 1103 28-29 0.6647853270698931 1103 30-31 0.749396904882623 1103 32-33 0.7858533529461056 1103 34-35 0.8131277040168499 1103 36-37 1.0324529055540594 1103 38-39 0.9152083144585745 1103 40-41 0.7641486718194415 1103 42-43 0.8120118781803498 1103 44-45 0.7973058427931434 1103 46-47 0.8136394188135085 1103 48-49 0.8447283602618114 1103 50-51 0.8225947710500847 1103 52-53 0.6342144339410041 1103 54-55 0.6324696032458377 1103 56-57 0.5621093710410321 1103 58-59 0.5525599384909619 1103 60-61 0.6608892847766477 1103 62-63 0.556203650528996 1103 64-65 0.5879594736482794 1103 66-67 0.5487179585094246 1103 68-69 0.5475872737927006 1103 70-71 0.5849968282590119 1103 72-73 0.6979830998260681 1103 74-75 0.5868113265345407 1103 76-77 0.3911511384596409 1103 78-79 0.17806911463783237 1103 80-81 0.07025793180227069 1103 82-83 -0.3283562585607527 1103 84-85 -0.3758217760273901 1103 86-87 -0.2647253772776139 1103 88-89 -1.004865119186757 1103 90-91 -6.784669339945616 1103 92-93 -10.527097606667159 1103 94-95 -9.044387216038851 1103 96-97 -5.582872147793992 1103 98-99 -4.1104843101466635 1103 100-101 -3.260922499316962 1104 1 1.9440341385161481 1104 2 1.1663145784295992 1104 3 0.9037409473696627 1104 4 0.6487859392137096 1104 5 0.6218150723430753 1104 6 0.7553109212680127 1104 7 0.732014473971148 1104 8 0.8318520351684242 1104 9 0.9872103148048339 1104 10-11 0.9476393378719266 1104 12-13 0.7860521106632063 1104 14-15 0.7977272831185829 1104 16-17 0.7849978248157186 1104 18-19 0.8766550791631147 1104 20-21 0.8905641902080035 1104 22-23 0.9291069319325018 1104 24-25 0.8015946690439719 1104 26-27 0.8239315449173219 1104 28-29 0.8440833529442813 1104 30-31 0.8874881580549356 1104 32-33 0.956698263392127 1104 34-35 0.9255679041276892 1104 36-37 0.867125811099406 1104 38-39 0.773516761557552 1104 40-41 0.8249070912062706 1104 42-43 0.8070865915784466 1104 44-45 0.7071602446483922 1104 46-47 0.8086895194523436 1104 48-49 0.7642364332468432 1104 50-51 0.6233171099550177 1104 52-53 0.4517431852901481 1104 54-55 0.6260028286476569 1104 56-57 0.7230526595973785 1104 58-59 0.6565461115897655 1104 60-61 0.7133846539315734 1104 62-63 0.7298594292402356 1104 64-65 0.7670865775834024 1104 66-67 0.6684622228249353 1104 68-69 0.42885714631684024 1104 70-71 0.4043318826623228 1104 72-73 0.5320265568430749 1104 74-75 0.4196966164910769 1104 76-77 0.5051077580156651 1104 78-79 0.13198263451764802 1104 80-81 -0.034694064201346464 1104 82-83 -0.39937546380564015 1104 84-85 -0.4014878702195084 1104 86-87 -0.1730250731732994 1104 88-89 -0.5385554286876477 1104 90-91 -3.4809241895707714 1104 92-93 -8.498761939679614 1104 94-95 -5.988415066743324 1104 96-97 -3.201264077726023 1104 98-99 -2.260052857592772 1104 100-101 -1.7371816698487414 1105 1 1.8822818870891318 1105 2 1.097654336723032 1105 3 0.8719471940833721 1105 4 0.7383646664733803 1105 5 0.7264705998591907 1105 6 0.6843261993410437 1105 7 0.6627395422606526 1105 8 0.7168289762129874 1105 9 0.8155158198503898 1105 10-11 0.7867477920191206 1105 12-13 0.6878532522765255 1105 14-15 0.8154450172924825 1105 16-17 0.7464855274162332 1105 18-19 0.846527078490837 1105 20-21 0.7239720336465751 1105 22-23 0.6753212407228446 1105 24-25 0.7400351326295436 1105 26-27 0.5369256346964448 1105 28-29 0.44885251877733623 1105 30-31 0.7180670905540723 1105 32-33 0.7719938339365484 1105 34-35 0.7345972801100942 1105 36-37 0.7463664412271811 1105 38-39 0.8591712572935748 1105 40-41 0.5995177936585492 1105 42-43 0.7021555260359449 1105 44-45 0.6738693965216314 1105 46-47 0.8591291021389509 1105 48-49 0.5243845847032915 1105 50-51 0.7299632179051017 1105 52-53 0.6021155472761563 1105 54-55 0.6309196508355015 1105 56-57 0.5734924038898654 1105 58-59 0.5048686352036889 1105 60-61 0.6241503308405747 1105 62-63 0.46825080136671815 1105 64-65 0.6585755745001087 1105 66-67 0.829170786746598 1105 68-69 0.7091873747114938 1105 70-71 0.49699360938002357 1105 72-73 0.43211783478271215 1105 74-75 0.4466937603275092 1105 76-77 0.3760405971092453 1105 78-79 0.11606092933466883 1105 80-81 0.03709993590119964 1105 82-83 -0.0443410806854061 1105 84-85 -0.17998618577203374 1105 86-87 0.17766325034639152 1105 88-89 0.06886388994375992 1105 90-91 -2.950521422172905 1105 92-93 -8.362891134472438 1105 94-95 -5.80358245444156 1105 96-97 -3.007165865718795 1105 98-99 -1.8833304143704268 1105 100-101 -1.3189824162622301 1106 1 1.577802405177266 1106 2 0.8845757128600376 1106 3 0.6639799659008148 1106 4 0.689917996642265 1106 5 0.7393539152155881 1106 6 0.7008821261788398 1106 7 0.6774598711112318 1106 8 -0.3142738012318844 1106 9 -0.6320685860440278 1106 10-11 -0.7539646263493722 1106 12-13 -0.3648737533631703 1106 14-15 0.3492558954950269 1106 16-17 0.5412045382725452 1106 18-19 0.7272518212067212 1106 20-21 0.5539951252836701 1106 22-23 0.5310084960957155 1106 24-25 0.6770225814864901 1106 26-27 0.6911387496516497 1106 28-29 0.40824107323958714 1106 30-31 0.6111534556674201 1106 32-33 0.7036305091560209 1106 34-35 0.7077575378330181 1106 36-37 0.6436538737586162 1106 38-39 0.6028278987107498 1106 40-41 0.5513875682752385 1106 42-43 0.6991716373363133 1106 44-45 0.6979752443584104 1106 46-47 0.8219387010377446 1106 48-49 0.5514604807270658 1106 50-51 0.30549805148709197 1106 52-53 0.5939568796393999 1106 54-55 0.633998299863066 1106 56-57 0.5899571878064265 1106 58-59 0.4915814285242597 1106 60-61 0.2574426459273944 1106 62-63 0.32570421624583545 1106 64-65 0.3286201912799065 1106 66-67 0.14583951376230786 1106 68-69 0.019731124414629875 1106 70-71 0.0837446843002887 1106 72-73 0.1880375635841567 1106 74-75 0.07412886483753667 1106 76-77 0.02969568775988307 1106 78-79 -0.17951910295776585 1106 80-81 -0.22749897390678342 1106 82-83 -0.5598378151108179 1106 84-85 -0.5117764592259633 1106 86-87 -0.31122203162102124 1106 88-89 -0.4942273004755826 1106 90-91 -3.7387967307298346 1106 92-93 -9.201923927994322 1106 94-95 -6.534086755538137 1106 96-97 -3.5187799431558346 1106 98-99 -2.3406074256249667 1106 100-101 -1.832582292704199 1107 1 1.7803286789176056 1107 2 1.0481960132521344 1107 3 0.6837629998147889 1107 4 0.6740321209355216 1107 5 0.6291908174277836 1107 6 0.6354074482390999 1107 7 0.4956455250997749 1107 8 0.5855735779470947 1107 9 0.5522106050844755 1107 10-11 0.5375701353526665 1107 12-13 0.47896326168032033 1107 14-15 0.7376144799369087 1107 16-17 0.6343222145513963 1107 18-19 0.7141473625321169 1107 20-21 0.5449870867613669 1107 22-23 0.5750083779214421 1107 24-25 0.48174599317786004 1107 26-27 0.5451627959168803 1107 28-29 0.4225732793600443 1107 30-31 0.6135922537281715 1107 32-33 0.8895803262608979 1107 34-35 0.6861538814706165 1107 36-37 0.4730028324970874 1107 38-39 0.4038963937471962 1107 40-41 0.5070999509436618 1107 42-43 0.6914595871901881 1107 44-45 0.4820046037516832 1107 46-47 0.3027090983186014 1107 48-49 0.40702876777992714 1107 50-51 0.40579564008754687 1107 52-53 0.5074322515387095 1107 54-55 0.38234609933074637 1107 56-57 0.34169471896664305 1107 58-59 0.29568195020397425 1107 60-61 0.03736737425620262 1107 62-63 0.06968954629229174 1107 64-65 0.21723932485816633 1107 66-67 0.2152813194526786 1107 68-69 0.2886491446246282 1107 70-71 0.2663886860207292 1107 72-73 0.10438706903580908 1107 74-75 -0.18926588297588864 1107 76-77 -0.10492484945220681 1107 78-79 -0.07253948189175574 1107 80-81 0.06613664199727154 1107 82-83 -0.2734626884702678 1107 84-85 -0.0772721555293856 1107 86-87 0.0829323567854523 1107 88-89 -0.019505634683437023 1107 90-91 -3.3483312026534655 1107 92-93 -4.7790423079758515 1107 94-95 -3.118224561830697 1107 96-97 -1.7024417836919064 1107 98-99 -1.0450061409197176 1107 100-101 -0.5975824113499151 1108 1 1.6730250593955596 1108 2 0.9716912274205285 1108 3 -0.24565782661991875 1108 4 -0.10220460363064632 1108 5 0.4006121461909302 1108 6 0.592916292893463 1108 7 0.5006676073405458 1108 8 0.5196971782400155 1108 9 0.663058171995921 1108 10-11 0.5350371183024265 1108 12-13 0.5066451572030672 1108 14-15 0.5562158385978293 1108 16-17 0.6135373049603814 1108 18-19 0.5386555506133206 1108 20-21 0.514558275137766 1108 22-23 0.38208383094302434 1108 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2311 78-79 1.0666787688724853 2311 80-81 1.386323232242571 2311 82-83 1.3301721343426394 2311 84-85 1.5561461403155228 2311 86-87 1.8685343028570642 2311 88-89 2.273511596689957 2311 90-91 2.9814372386609627 2311 92-93 3.9302644756949228 2311 94-95 3.605096402194768 2311 96-97 2.6591069379350962 2311 98-99 2.474164258876904 2311 100-101 2.4982517754823164 2312 1 1.2399426586691646 2312 2 0.5010191217126092 2312 3 -0.12460198125065247 2312 4 -0.3936624303233529 2312 5 -0.10947161253972837 2312 6 -0.24892622311391222 2312 7 0.08310961246215243 2312 8 0.13324462766872358 2312 9 0.17426792844311478 2312 10-11 0.0073677900878834635 2312 12-13 0.11804434522867524 2312 14-15 0.17077567393210558 2312 16-17 -0.09515109416464185 2312 18-19 -0.4206244929348628 2312 20-21 0.23812094882641333 2312 22-23 0.3955352891357151 2312 24-25 0.34438761297833054 2312 26-27 0.28309455247723747 2312 28-29 0.16575095082294666 2312 30-31 0.1734821991558846 2312 32-33 0.3488045606492136 2312 34-35 0.3391921394667605 2312 36-37 0.27538772800026123 2312 38-39 0.1566082246786067 2312 40-41 0.15679378991674753 2312 42-43 0.11660053548212801 2312 44-45 0.1558563097150909 2312 46-47 0.23745004095724198 2312 48-49 0.23976826522401495 2312 50-51 0.05423713815479658 2312 52-53 0.00670749101218604 2312 54-55 0.17427612053275254 2312 56-57 0.22988337216314392 2312 58-59 0.17862721228844336 2312 60-61 0.2228900249515533 2312 62-63 0.17549492065370842 2312 64-65 0.1853652928670293 2312 66-67 0.2926360754890247 2312 68-69 0.35871274198226644 2312 70-71 0.20701298093675646 2312 72-73 0.21298245775326663 2312 74-75 0.3152539734507158 2312 76-77 0.5095836362165187 2312 78-79 0.5813715389173346 2312 80-81 0.6372531647562063 2312 82-83 0.6901528332451861 2312 84-85 0.8639180453275621 2312 86-87 1.4282195998392417 2312 88-89 1.6697468539001967 2312 90-91 2.3914893947796685 2312 92-93 3.5250770084749554 2312 94-95 3.0948037639681587 2312 96-97 2.5149652104815274 2312 98-99 2.1925732584311213 2312 100-101 2.2260858258595704 2313 1 -7.4624642905017105 2313 2 -2.7484221656146417 2313 3 -1.5207920517241647 2313 4 -0.6613232588655364 2313 5 -0.4575211686481282 2313 6 -0.13792706347101813 2313 7 0.07114512379033755 2313 8 0.0791800782582186 2313 9 0.10827549997747354 2313 10-11 0.03256698800446145 2313 12-13 -0.16552725569322035 2313 14-15 -0.23366031332109571 2313 16-17 -0.332581931110397 2313 18-19 -0.4977908087593761 2313 20-21 -0.2540903885062491 2313 22-23 -0.8608983018516554 2313 24-25 -0.09372622628881544 2313 26-27 -0.1015464486293709 2313 28-29 -0.28845953783223166 2313 30-31 0.18239793879494925 2313 32-33 -0.07706665670374235 2313 34-35 -0.004060192316210021 2313 36-37 0.18104943850139676 2313 38-39 0.020033686667012773 2313 40-41 0.10384763101436079 2313 42-43 0.14008487239043887 2313 44-45 0.07650083865748769 2313 46-47 -0.9482360981426581 2313 48-49 -0.43138392261659675 2313 50-51 -0.28481314182557327 2313 52-53 -0.5602781563540056 2313 54-55 -0.35578363634611776 2313 56-57 -0.21409779052499545 2313 58-59 -0.17074361816743178 2313 60-61 0.023658860183317643 2313 62-63 0.07214444181656177 2313 64-65 0.057621660028207344 2313 66-67 0.1694142295323111 2313 68-69 0.08515813125065819 2313 70-71 0.22649751753389324 2313 72-73 0.1458229812707863 2313 74-75 0.15700594329770468 2313 76-77 0.5363043298023484 2313 78-79 0.48584802971916474 2313 80-81 0.48033895606474886 2313 82-83 0.7403500758754191 2313 84-85 0.8821500670936437 2313 86-87 1.2976568601086527 2313 88-89 1.827235101381202 2313 90-91 2.4932083826901703 2313 92-93 3.384558583021292 2313 94-95 3.1264889212647056 2313 96-97 2.077772668858888 2313 98-99 1.677354640776965 2313 100-101 1.3323546236496782 2314 1 0.7265007301613657 2314 2 0.08038782703285463 2314 3 -1.2438306143514595 2314 4 -0.2489339309860199 2314 5 -0.152112665094414 2314 6 -3.252161143604887 2314 7 -2.702552375512443 2314 8 -2.2427060846631726 2314 9 -2.9105158832233187 2314 10-11 -2.229884827726522 2314 12-13 -0.9586385489737097 2314 14-15 -1.0658456302817214 2314 16-17 -1.9449899191435591 2314 18-19 -2.9358417354286885 2314 20-21 -2.562132644288951 2314 22-23 -3.61583619430224 2314 24-25 -2.7399020948564257 2314 26-27 -1.68389952104733 2314 28-29 -1.3960696690786918 2314 30-31 -1.5302236606709414 2314 32-33 -1.9481640055759328 2314 34-35 -1.3292866579580362 2314 36-37 -1.6314307562132413 2314 38-39 -2.9607064318182026 2314 40-41 -2.193478930125522 2314 42-43 -1.2412579487746385 2314 44-45 -1.7962195379260457 2314 46-47 -1.2718540382577608 2314 48-49 -1.9823804998981984 2314 50-51 -2.905310948750447 2314 52-53 -1.8370393013166932 2314 54-55 -2.304620064211271 2314 56-57 -1.3950341448650931 2314 58-59 -0.7395962722531415 2314 60-61 -1.0313994443456558 2314 62-63 -0.7456455469434005 2314 64-65 -1.24408121421013 2314 66-67 -0.6176441426059149 2314 68-69 -0.5483408334826692 2314 70-71 -0.5638601185086003 2314 72-73 -1.5333599947194791 2314 74-75 -1.5653678513560934 2314 76-77 -0.7301908910686237 2314 78-79 -1.607155562022367 2314 80-81 -0.5130682291085442 2314 82-83 -0.14140874501921985 2314 84-85 -1.374365284432212 2314 86-87 0.03683408931247101 2314 88-89 0.9433933746409018 2314 90-91 2.1392840881504647 2314 92-93 3.319348174576895 2314 94-95 3.005178282103735 2314 96-97 2.029208604866735 2314 98-99 1.8702876146544405 2314 100-101 1.780512795495497 2315 1 -2.6258950683956854 2315 2 -1.8554199042429786 2315 3 -2.779823398089434 2315 4 -3.9200922503652826 2315 5 -3.7594389190749276 2315 6 -3.026894179730551 2315 7 -5.91566033567927 2315 8 -2.993388035507543 2315 9 -2.6144736844092265 2315 10-11 -3.793080112881313 2315 12-13 -2.9504105671278964 2315 14-15 -2.1151446312002307 2315 16-17 -2.5278089174081018 2315 18-19 -1.973439536402637 2315 20-21 -1.4240394584836338 2315 22-23 -0.8641327962607974 2315 24-25 -2.2538698099819 2315 26-27 -3.7272517552552813 2315 28-29 -3.12374648176273 2315 30-31 -3.025465485082634 2315 32-33 -3.7207797038350208 2315 34-35 -3.615026003594469 2315 36-37 -2.8471273238936874 2315 38-39 -1.7846023218779337 2315 40-41 -1.3542322588666664 2315 42-43 -2.67704259567774 2315 44-45 -6.160804357134396 2315 46-47 -2.9966945219755488 2315 48-49 -2.0166112128140448 2315 50-51 -3.1630967160441443 2315 52-53 -3.1705708118121336 2315 54-55 -1.7065852702541804 2315 56-57 -1.250642736404032 2315 58-59 -2.7302959559609405 2315 60-61 -2.8718127475357385 2315 62-63 -4.723434460390287 2315 64-65 -4.113438074148895 2315 66-67 -3.3985369677433184 2315 68-69 -1.8048316255401637 2315 70-71 -3.3592531237839616 2315 72-73 -2.540705035604585 2315 74-75 -1.4800604230445025 2315 76-77 -3.0851184573158754 2315 78-79 -1.5880887412566231 2315 80-81 -1.2802316961577453 2315 82-83 -1.0614630615319598 2315 84-85 -2.4350630291978845 2315 86-87 -1.8202432998856253 2315 88-89 -1.1497952617789942 2315 90-91 -0.2912247995871091 2315 92-93 0.3760904701493466 2315 94-95 0.2728358853955939 2315 96-97 -0.07764416539955121 2315 98-99 -0.5430230887343832 2315 100-101 -0.9120207819224113 2316 1 -7.509547932288957 2316 2 -6.037611576020527 2316 3 -3.185224002395792 2316 4 -3.8925748208397657 2316 5 -1.9835017610615253 2316 6 -2.668974800313606 2316 7 -2.9932351703246383 2316 8 -2.449648318296795 2316 9 -6.717163971816046 2316 10-11 -6.725297231645037 2316 12-13 -5.868026232565995 2316 14-15 -4.607137872730682 2316 16-17 -4.618224500478426 2316 18-19 -3.9680485640637215 2316 20-21 -2.5842990700817765 2316 22-23 -3.175715694104994 2316 24-25 -2.92401835866783 2316 26-27 -3.8910921073868856 2316 28-29 -3.8877678492630636 2316 30-31 -6.6259254603519935 2316 32-33 -4.650183202262461 2316 34-35 -5.251859350168829 2316 36-37 -3.905622660591881 2316 38-39 -6.027729311595948 2316 40-41 -3.6196229984135044 2316 42-43 -4.449145754127198 2316 44-45 -2.989732725372086 2316 46-47 -3.963586098597382 2316 48-49 -4.9658787887915565 2316 50-51 -4.2864092566704635 2316 52-53 -5.996238177089381 2316 54-55 -4.735776615419756 2316 56-57 -3.809151275068956 2316 58-59 -2.7590594870980105 2316 60-61 -3.0507006076086363 2316 62-63 -3.8970205590474123 2316 64-65 -2.8528600373625927 2316 66-67 -3.8369009300300654 2316 68-69 -3.037390792419508 2316 70-71 -2.286664609479395 2316 72-73 -1.8070599093137112 2316 74-75 -1.8804985721037717 2316 76-77 -2.721444426529672 2316 78-79 -4.618753550898713 2316 80-81 -5.488916043616946 2316 82-83 -3.1428434997950703 2316 84-85 -1.7671507686876176 2316 86-87 -1.7768978840185952 2316 88-89 -0.7174681917242829 2316 90-91 -0.411561663782539 2316 92-93 -0.3470595222380375 2316 94-95 -1.0014191155133076 2316 96-97 -2.736086748913511 2316 98-99 -4.551126674646337 2316 100-101 -4.0383312626513295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22789.0 3 7285.0 4 6692.0 5 7251.0 6 8273.0 7 9708.0 8 11410.0 9 13810.0 10 16535.0 11 19781.0 12 23774.0 13 27172.0 14 30458.0 15 34460.0 16 39205.0 17 42357.0 18 40109.0 19 39092.0 20 42804.0 21 47549.0 22 52896.0 23 60193.0 24 69032.0 25 79830.0 26 92134.0 27 106174.0 28 124541.0 29 144501.0 30 168722.0 31 195731.0 32 224162.0 33 252843.0 34 294403.0 35 348599.0 36 384663.0 37 319582.0 38 102636.0 39 2059.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52317192981656 19.745098938971555 13.31209205327402 23.419637077937868 2 15.569178474711173 20.20572685052858 40.093111689857196 24.131982984903047 3 19.441420388155834 24.23052808572487 29.623329248482435 26.704722277636865 4 12.759773740257327 16.313879244151547 37.462883358120514 33.46346365747061 5 13.680149859485358 37.206812302590535 34.66569313859352 14.447344699330586 6 32.11806282968741 36.274956625984736 17.604420429952143 14.002560114375717 7 28.22017760617981 32.451660378574246 21.20659526951839 18.12156674572756 8 26.057761191519496 35.10442415412031 20.153615155919983 18.684199498440215 9 26.168836870891667 16.740907494332152 20.346320346320347 36.74393528845584 10-11 24.73943303102115 26.497927765800117 27.951820109402565 20.81081909377617 12-13 25.655210139583755 23.791494646776165 28.08671598339031 22.46657923024977 14-15 22.4441406535628 25.343956226746204 26.256839749074622 25.955063370616372 16-17 22.18839855498604 28.503521744050776 26.887872310160905 22.420207390802275 18-19 22.132244361736657 27.26967798845313 28.90986376593554 21.688213883874678 20-21 22.719522786097112 26.51085232816268 29.06974423483431 21.699880650905897 22-23 22.27535190871144 26.481543197543893 28.99203853287911 22.251066360865558 24-25 22.087701819234724 26.58741405365776 29.203691672199707 22.121192454907813 26-27 21.939015328265043 26.863700680928122 28.94408851594817 22.25319547485867 28-29 21.828989261545836 27.296157664700043 28.905171233267524 21.969681840486597 30-31 22.13263678344803 26.90155779485079 28.800148796109394 22.16565662559179 32-33 21.79544745903994 26.91289820041895 28.8296119131849 22.46204242735621 34-35 22.32331371816338 27.035104783800513 28.700934739388266 21.940646758647837 36-37 22.352232715804092 26.77079550618466 28.762121209095685 22.11485056891556 38-39 21.95311665472732 27.075403578723893 28.730777661577317 22.240702104971472 40-41 22.331395913519376 26.939800406728377 28.704814435705384 22.023989244046867 42-43 22.059087363216435 26.900594553044144 28.66333237782712 22.376985705912304 44-45 22.059741008310443 27.058127461763654 28.69226580010019 22.189865729825716 46-47 22.238989846435757 27.18003835968422 28.276646688139873 22.304325105740148 48-49 22.0396532017714 27.273514581496684 28.259030603444156 22.42780161328776 50-51 22.063146690712763 27.435295259075787 28.260476888921865 22.241081161289586 52-53 22.265630406255802 27.33274520404248 28.08718296200003 22.314441427701688 54-55 21.905962172448913 27.39145930931518 28.276395490553668 22.42618302768224 56-57 21.994389310848355 27.45509016628802 28.17754449117879 22.372976031684832 58-59 22.01898541068371 27.218284580319835 28.11209577121081 22.650634237785646 60-61 21.762607266147818 27.404903611626384 28.304729977936425 22.52775914428937 62-63 21.825658075322803 27.27024097713543 28.39563843605059 22.508462511491178 64-65 21.6557983729144 27.324717125152237 28.26933253214395 22.750151969789417 66-67 21.674091545620225 27.307121894456436 28.1107200611483 22.908066498775035 68-69 21.969016143628465 27.08484909502075 28.238101906529923 22.708032854820857 70-71 21.902295777827565 27.06433394718937 27.93135270756717 23.102017567415896 72-73 21.866735825741955 27.089301655966125 28.004132996553743 23.039829521738177 74-75 21.81026480368298 27.368030769674107 27.85710607169234 22.964598354950578 76-77 21.861443696234925 27.12721787621498 27.87985044941082 23.13148797813927 78-79 21.97150665465355 27.24278267742711 27.741469723591294 23.044240944328045 80-81 22.02642888932997 27.162421074275976 27.684580493786186 23.126569542607868 82-83 21.99189675193713 27.15658298295076 27.785430877102257 23.066089388009853 84-85 21.943108889198616 27.11291671618084 27.770583857375208 23.173390537245336 86-87 21.772788209012354 27.061410585576233 27.917427592071796 23.248373613339616 88-89 21.968248035118908 27.074898742105162 27.54036405762034 23.416489165155586 90-91 21.77463067002558 27.00578566454238 27.85655394178772 23.36302972364432 92-93 22.084986079002555 26.662324556066906 27.889090089251845 23.36359927567869 94-95 21.72764484220712 26.67551941216018 27.991221701726353 23.605614043906343 96-97 22.08915954301499 26.497058608200273 27.59998955338882 23.813792295395913 98-99 22.154411598857155 26.548023216672284 27.297648553897336 23.999916630573225 100-101 22.300551298802638 26.51582897643412 27.36991305810419 23.81370666665905 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4344.0 1 2527.5 2 688.5 3 675.0 4 619.0 5 572.0 6 749.5 7 1261.0 8 1914.0 9 2247.0 10 2159.0 11 1991.0 12 2058.0 13 2297.0 14 2561.5 15 2830.0 16 3087.5 17 3439.0 18 3866.0 19 4508.5 20 5362.0 21 6443.5 22 8014.5 23 10148.0 24 12917.5 25 16315.5 26 20965.0 27 26691.5 28 31878.0 29 36978.5 30 42888.0 31 50122.5 32 58752.5 33 64849.0 34 69942.0 35 76202.0 36 83222.5 37 90707.0 38 97063.5 39 102131.0 40 106935.5 41 112059.5 42 116344.5 43 121314.0 44 125953.0 45 129972.0 46 132402.5 47 135104.5 48 137662.5 49 135124.5 50 130978.5 51 127295.0 52 122940.0 53 119236.0 54 114966.0 55 107028.5 56 98016.5 57 88792.5 58 79272.0 59 69062.5 60 57134.5 61 46241.0 62 37117.0 63 28054.5 64 20335.5 65 15378.5 66 11577.0 67 8642.0 68 6416.0 69 4708.0 70 3518.5 71 2537.0 72 1822.0 73 1391.5 74 1094.5 75 866.0 76 636.0 77 422.5 78 283.0 79 204.0 80 160.0 81 117.5 82 67.0 83 47.0 84 43.0 85 35.5 86 20.0 87 11.0 88 10.5 89 9.5 90 7.0 91 5.0 92 6.0 93 3.0 94 3.5 95 5.0 96 2.0 97 0.5 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1320158316527739 2 0.11658836706549415 3 0.12282197360537286 4 0.13323978179530716 5 0.12319200504381314 6 0.13600078560520776 7 0.1648632378035503 8 0.15250988055100526 9 0.16255765730249927 10-11 0.15733452122913058 12-13 0.14636166588153587 14-15 0.15141401821408595 16-17 0.14664630544956686 18-19 0.16183182640402025 20-21 0.16671339499575175 22-23 0.19966042499533904 24-25 0.2208091449000417 26-27 0.2339594929430735 28-29 0.2292771720489637 30-31 0.22051027335360918 32-33 0.2216772955825362 34-35 0.2113448792630112 36-37 0.21877397198862011 38-39 0.20780111664102538 40-41 0.21164375080944378 42-43 0.20550976811837593 44-45 0.198208763198381 46-47 0.21208494213989182 48-49 0.24924463774633776 50-51 0.25147905835538104 52-53 0.25285956026033135 54-55 0.2515644502257903 56-57 0.22604651295181194 58-59 0.22613190482222126 60-61 0.1987638103560414 62-63 0.19129202169522785 64-65 0.19245904392415494 66-67 0.1988207382696476 68-69 0.19930462553530026 70-71 0.20595095944882397 72-73 0.23488457153917425 74-75 0.23726131193223302 76-77 0.2518775537506244 78-79 0.2479922236470014 80-81 0.26211034622133855 82-83 0.261526835106875 84-85 0.23700513632100512 86-87 0.240136171569346 88-89 0.24336683066649775 90-91 0.43433151685849 92-93 0.5775336835348819 94-95 0.3373832799871343 96-97 0.27571611757321995 98-99 0.30547518441086013 100-101 0.32430409183611025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3513215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.52337191414677 #Duplication Level Percentage of deduplicated Percentage of total 1 74.57716109188026 42.15352612697584 2 13.765986873915177 15.561999916791402 3 4.9992204183466855 8.477183849610183 4 2.3619700275442774 5.3402604126781075 5 1.2957569487344454 3.6620275961828526 6 0.7584678076015178 2.5722694784380864 7 0.4893638005972258 1.9362344471734145 8 0.3374451940427146 1.5258832162814226 9 0.244162075195139 1.242077740522624 >10 1.083894696870209 10.585490402429357 >50 0.05745627589275055 2.220510536706538 >100 0.025947545464076092 2.6244697632868617 >500 0.002094467756892727 0.792322502666397 >1k 9.706070007254113E-4 0.8722766149766127 >5k 1.021691579710959E-4 0.4334673952805093 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8840 0.25162137813939656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6172 0.175679541388728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4196 0.11943476274580406 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8463956803099154E-5 0.0 12-13 8.539187040929746E-5 0.0 0.0 2.8463956803099154E-5 2.8463956803099154E-5 14-15 1.1385582721239662E-4 0.0 0.0 2.8463956803099154E-5 8.539187040929746E-5 16-17 1.1385582721239662E-4 0.0 0.0 4.269593520464873E-5 1.1385582721239662E-4 18-19 1.1385582721239662E-4 0.0 0.0 1.1385582721239662E-4 1.1385582721239662E-4 20-21 1.1385582721239662E-4 0.0 0.0 1.7078374081859493E-4 1.1385582721239662E-4 22-23 1.1385582721239662E-4 0.0 0.0 2.561756112278924E-4 1.1385582721239662E-4 24-25 1.1385582721239662E-4 0.0 0.0 7.40062876880578E-4 1.1385582721239662E-4 26-27 1.1385582721239662E-4 0.0 0.0 0.0017078374081859493 1.1385582721239662E-4 28-29 1.280878056139462E-4 0.0 0.0 0.005123512224557848 1.1385582721239662E-4 30-31 1.4231978401549577E-4 0.0 0.0 0.016679878686616106 1.1385582721239662E-4 32-33 1.4231978401549577E-4 0.0 0.0 0.04631085771864232 1.1385582721239662E-4 34-35 1.4231978401549577E-4 0.0 0.0 0.09694823687135573 1.1385582721239662E-4 36-37 1.4231978401549577E-4 0.0 0.0 0.18502995119854607 1.1385582721239662E-4 38-39 2.2771165442479323E-4 0.0 0.0 0.3234217091752142 1.1385582721239662E-4 40-41 2.2771165442479323E-4 0.0 0.0 0.4725586108450522 1.1385582721239662E-4 42-43 2.561756112278924E-4 0.0 0.0 0.6254812187697023 1.1385582721239662E-4 44-45 2.8463956803099154E-4 0.0 0.0 0.8142257163310529 1.5655176241704535E-4 46-47 2.8463956803099154E-4 0.0 0.0 1.0431328569415763 1.9924769762169408E-4 48-49 2.8463956803099154E-4 0.0 0.0 1.2847206903078803 1.9924769762169408E-4 50-51 2.8463956803099154E-4 0.0 0.0 1.5422625714623215 1.9924769762169408E-4 52-53 2.8463956803099154E-4 0.0 0.0 1.7930442628760266 1.9924769762169408E-4 54-55 3.131035248340907E-4 0.0 0.0 2.063964203727924 1.9924769762169408E-4 56-57 3.4156748163718985E-4 0.0 0.0 2.3764842174475516 1.9924769762169408E-4 58-59 3.4156748163718985E-4 0.0 0.0 2.7155895668212735 1.9924769762169408E-4 60-61 3.4156748163718985E-4 0.0 0.0 3.0721860176504996 2.2771165442479323E-4 62-63 3.4156748163718985E-4 1.4231978401549577E-5 0.0 3.4200724976979773 2.561756112278924E-4 64-65 3.4156748163718985E-4 2.8463956803099154E-5 0.0 3.777779042842525 2.561756112278924E-4 66-67 3.70031438440289E-4 2.8463956803099154E-5 0.0 4.181027918872031 2.561756112278924E-4 68-69 3.70031438440289E-4 2.8463956803099154E-5 0.0 4.6254641403956205 2.561756112278924E-4 70-71 3.70031438440289E-4 2.8463956803099154E-5 0.0 5.087448391288321 2.561756112278924E-4 72-73 3.70031438440289E-4 2.8463956803099154E-5 0.0 5.552150950055719 3.131035248340907E-4 74-75 3.842634168418386E-4 2.8463956803099154E-5 0.0 6.007830434516532 3.131035248340907E-4 76-77 3.9849539524338816E-4 2.8463956803099154E-5 0.0 6.490223342437055 3.131035248340907E-4 78-79 3.9849539524338816E-4 2.8463956803099154E-5 0.0 7.019852186672321 3.131035248340907E-4 80-81 4.5542330884958647E-4 2.8463956803099154E-5 0.0 7.556312380540332 3.273355032356403E-4 82-83 4.5542330884958647E-4 2.8463956803099154E-5 0.0 8.072306989466913 4.5542330884958647E-4 84-85 4.5542330884958647E-4 2.8463956803099154E-5 0.0 8.564961153814952 4.5542330884958647E-4 86-87 4.5542330884958647E-4 2.8463956803099154E-5 0.0 9.06209839135948 4.5542330884958647E-4 88-89 4.5542330884958647E-4 2.8463956803099154E-5 0.0 9.599597519650803 4.5542330884958647E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10625 0.0 37.81546 1 GGTATCA 6275 0.0 37.647015 1 TCAACGC 13165 0.0 31.314453 4 ATCAACG 13180 0.0 31.24282 3 TATCAAC 13255 0.0 31.17208 2 CAACGCA 13450 0.0 30.509825 5 AACGCAG 13805 0.0 29.862713 6 ACGCAGA 15515 0.0 26.265598 7 CGCAGAG 15690 0.0 25.942408 8 TACATGG 10840 0.0 25.382011 2 GTACATG 10675 0.0 25.284788 1 ACATGGG 11175 0.0 23.688229 3 GTATAGG 970 0.0 23.473854 1 GCAGAGT 16830 0.0 23.029139 9 AGAGTAC 14130 0.0 21.099335 10-11 CATGGGG 6500 0.0 20.508736 4 GAGTACT 9750 0.0 20.48178 12-13 AGTACTT 10320 0.0 19.67226 12-13 GTACTTT 10360 0.0 19.209318 14-15 CAGAGTA 16285 0.0 18.656794 10-11 >>END_MODULE