FastQCFastQC Report
Fri 27 May 2016
SRR939727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513215
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.26206765028613394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73820.21012092912047797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55040.15666561824425776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.040.7327041
GTATCAA103900.035.268061
ATCAACG129400.027.9099623
TCAACGC129650.027.855354
CAACGCA133200.027.1483575
TATCAAC134500.027.1336462
AACGCAG137600.026.3665076
GTACATG104350.023.5010341
CGCAGAG158600.022.7865568
ACGCAGA158950.022.7657017
TACATGG104050.022.6577842
ACATGGG106100.021.5966113
GCAGAGT176600.019.7703369
AGAGTAC149800.019.59578910-11
GTAAGGT15950.019.2134364
GAGTACT103500.019.16626712-13
TAAGGTG18450.019.1653755
AGTACTT111100.018.49133312-13
GGTAAGG16200.018.3353963
GTACTTT110750.017.69911614-15
Produced by FastQC (version 0.11.5)