##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939727_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3513215 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.273385204150614 31.0 31.0 34.0 30.0 34.0 2 31.500750452221112 31.0 31.0 34.0 30.0 34.0 3 31.559986223444906 31.0 31.0 34.0 30.0 34.0 4 35.38976920000626 37.0 35.0 37.0 33.0 37.0 5 35.10792906212686 37.0 35.0 37.0 32.0 37.0 6 35.13121200951265 37.0 35.0 37.0 32.0 37.0 7 35.043995884111844 37.0 35.0 37.0 32.0 37.0 8 34.98210897995141 37.0 35.0 37.0 32.0 37.0 9 36.5726000259022 38.0 35.0 39.0 32.0 39.0 10-11 36.41816000444038 38.0 35.0 39.0 32.0 39.0 12-13 36.35619895167247 38.0 35.0 39.0 32.0 39.0 14-15 37.37020393001852 39.0 36.0 41.0 32.0 41.0 16-17 37.2874610577491 39.0 36.0 40.0 32.0 41.0 18-19 37.25076532463854 39.0 36.0 40.0 31.5 41.0 20-21 37.15784075270087 39.0 36.0 40.0 31.0 41.0 22-23 37.006892689459654 39.0 36.0 40.0 31.0 41.0 24-25 36.837304577146575 39.0 36.0 40.0 30.5 41.0 26-27 36.51593711173384 38.0 35.0 40.0 30.0 41.0 28-29 36.27797145919051 38.0 35.0 40.0 30.0 41.0 30-31 36.011102366351054 38.0 34.5 40.0 29.0 41.0 32-33 35.95988047984538 38.0 34.0 40.0 29.0 41.0 34-35 36.0424666580326 38.0 35.0 40.0 30.0 41.0 36-37 36.00293378002769 38.0 35.0 40.0 29.0 41.0 38-39 35.85701842898884 38.0 34.5 40.0 29.0 41.0 40-41 35.6732481501986 38.0 34.0 40.0 28.0 41.0 42-43 35.46416060502986 38.0 34.0 40.0 27.0 41.0 44-45 35.21657797772126 38.0 34.0 40.0 27.0 41.0 46-47 34.92426908116924 38.0 33.5 40.0 26.0 41.0 48-49 34.60937033457958 38.0 33.0 40.0 24.5 41.0 50-51 34.33043380493366 38.0 33.0 40.0 24.0 41.0 52-53 34.025854523563176 37.0 33.0 40.0 24.0 41.0 54-55 33.687694177555315 37.0 32.5 40.0 23.0 41.0 56-57 32.336686197685026 36.5 30.5 39.5 15.0 40.5 58-59 28.805835111144635 34.0 23.0 38.0 7.0 40.0 60-61 28.797538294695883 33.5 23.5 38.0 7.5 40.0 62-63 30.646617841492763 34.5 28.5 38.0 7.5 40.0 64-65 30.130796578063112 34.5 28.0 38.0 2.0 40.0 66-67 24.584350089590302 28.0 16.5 36.0 2.0 39.0 68-69 16.959841199584993 13.0 7.0 28.5 2.0 35.0 70-71 15.77815775578779 12.0 7.5 25.0 2.0 33.0 72-73 17.75679128661354 16.0 7.0 29.0 2.0 34.0 74-75 18.460823063774917 17.5 7.5 30.0 2.0 34.5 76-77 19.61446239413187 22.0 7.5 30.5 2.0 34.5 78-79 22.618423723000156 27.5 9.5 33.5 2.0 35.5 80-81 25.20015285144803 30.5 19.5 34.0 2.0 36.0 82-83 26.151457425748212 32.0 24.0 35.0 2.0 36.0 84-85 26.132600196685942 33.0 24.0 35.0 2.0 36.0 86-87 25.818931093030173 32.0 22.5 35.0 2.0 35.5 88-89 25.330084979143038 32.0 19.5 34.5 2.0 35.0 90-91 23.62296941690161 31.5 7.0 34.0 2.0 35.0 92-93 21.42700219030148 29.5 2.0 34.0 2.0 35.0 94-95 17.04099820250113 18.0 2.0 32.0 2.0 34.5 96-97 15.685963853621256 9.5 2.0 31.5 2.0 34.0 98-99 14.562786934474548 2.0 2.0 31.0 2.0 34.0 100-101 13.902213214961225 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3234156991536281 1101 2 0.3766024100540264 1101 3 0.36960118197357517 1101 4 0.29091069815768833 1101 5 0.3822835463685905 1101 6 0.4121867550834608 1101 7 0.3829868683887625 1101 8 0.38103819425064245 1101 9 0.4932574621314174 1101 10-11 0.42294036421255043 1101 12-13 0.5173328204156391 1101 14-15 0.4585091834773962 1101 16-17 0.4415155885022628 1101 18-19 0.4988305647823381 1101 20-21 0.5135712561294739 1101 22-23 0.5194512425622833 1101 24-25 0.4394117258528709 1101 26-27 0.5873226307794752 1101 28-29 0.5203614625338346 1101 30-31 0.5488461377533866 1101 32-33 0.5194480171030591 1101 34-35 0.599272021028753 1101 36-37 0.5601095839252537 1101 38-39 0.4428781230500647 1101 40-41 0.46621432090319814 1101 42-43 0.6962066339548869 1101 44-45 0.46890367906512154 1101 46-47 0.4723513383023743 1101 48-49 0.35581399501191413 1101 50-51 0.3088259900391037 1101 52-53 0.4025276770837465 1101 54-55 0.2371555669915466 1101 56-57 0.8404084044931039 1101 58-59 -8.391443566950429 1101 60-61 -9.832608325187103 1101 62-63 -2.881223152862301 1101 64-65 -0.3469299799007004 1101 66-67 -1.4020238987505564 1101 68-69 -3.7061024619569487 1101 70-71 -4.6407863771963545 1101 72-73 -6.974316805726099 1101 74-75 -7.760514180538234 1101 76-77 -8.761573451279578 1101 78-79 -11.845025229077013 1101 80-81 -8.334643817992177 1101 82-83 -4.903504393849712 1101 84-85 -3.92355131564128 1101 86-87 -3.842812555407246 1101 88-89 -4.057562677248352 1101 90-91 -9.562775106507967 1101 92-93 -14.285992723310699 1101 94-95 -12.131890461488503 1101 96-97 -11.764481587642862 1101 98-99 -11.140932584806354 1101 100-101 -10.842331792343582 1102 1 0.5455286679266216 1102 2 0.5744365121481181 1102 3 0.5208239021694858 1102 4 0.3966311229714279 1102 5 0.563567870370818 1102 6 0.5977266649395219 1102 7 0.593490762454195 1102 8 0.6003761758766828 1102 9 0.7380101930639427 1102 10-11 0.7464568588088198 1102 12-13 0.7485367106660377 1102 14-15 0.8277037377808583 1102 16-17 0.8247413134960411 1102 18-19 0.8653615174703972 1102 20-21 0.8813290392644504 1102 22-23 0.8871382885044454 1102 24-25 0.7797901278236097 1102 26-27 0.8913716367799935 1102 28-29 0.8378081836889564 1102 30-31 0.8638918818950287 1102 32-33 0.6750584870251046 1102 34-35 0.6936908451608801 1102 36-37 0.8003624983430129 1102 38-39 0.6920035025102607 1102 40-41 0.8070476890563398 1102 42-43 0.8214584719864746 1102 44-45 0.725792891223108 1102 46-47 0.8257166574122792 1102 48-49 0.8091504969475878 1102 50-51 0.7661410332853009 1102 52-53 0.7757583389204541 1102 54-55 0.7081571783916161 1102 56-57 1.2129702989691111 1102 58-59 -8.7897989896659 1102 60-61 -9.860895481402075 1102 62-63 -2.7763154677375006 1102 64-65 0.05418339853487808 1102 66-67 -1.1638175941250921 1102 68-69 -3.7566013238034657 1102 70-71 -4.157185938103462 1102 72-73 -6.549132684647642 1102 74-75 -7.385285164618619 1102 76-77 -8.448216997595067 1102 78-79 -11.5620342198537 1102 80-81 -7.78486561258681 1102 82-83 -3.8613512717847946 1102 84-85 -2.9012659207029294 1102 86-87 -2.650403757562561 1102 88-89 -2.76603449500573 1102 90-91 -8.301018426101102 1102 92-93 -13.874496759985947 1102 94-95 -11.890386077826317 1102 96-97 -11.684194987333662 1102 98-99 -11.154488805773447 1102 100-101 -10.791926716887549 1103 1 0.2173713050616506 1103 2 0.25791018628249773 1103 3 0.1891053392798483 1103 4 0.16778439093017283 1103 5 0.22461496243489876 1103 6 0.13193654205379346 1103 7 0.26381290706979144 1103 8 0.26651941536252366 1103 9 0.2877645243877254 1103 10-11 0.3325855245657934 1103 12-13 0.2616673609170945 1103 14-15 0.435863484094547 1103 16-17 0.37953646218213066 1103 18-19 0.41667938472708244 1103 20-21 0.3643988441171828 1103 22-23 0.4121314947119501 1103 24-25 0.3130266295376387 1103 26-27 0.18487246681119274 1103 28-29 0.36470011838118666 1103 30-31 0.33745776530005855 1103 32-33 0.33999795642187536 1103 34-35 0.27706585672478923 1103 36-37 -0.01955140840568248 1103 38-39 0.09713742534562186 1103 40-41 0.24216440095071334 1103 42-43 0.2987711399842752 1103 44-45 0.21316478888515178 1103 46-47 0.051698031178148085 1103 48-49 0.09146999728422145 1103 50-51 0.1501003270649477 1103 52-53 0.10116614314102179 1103 54-55 0.07770528036980551 1103 56-57 0.5421208725386535 1103 58-59 -6.774029521194237 1103 60-61 -8.900434470382958 1103 62-63 -2.976582979339952 1103 64-65 -0.8374816299123218 1103 66-67 -2.1716137520828553 1103 68-69 -4.474787342660335 1103 70-71 -4.902208882812589 1103 72-73 -7.752362080986254 1103 74-75 -8.464309739837947 1103 76-77 -9.256414744176006 1103 78-79 -12.381116409003162 1103 80-81 -9.14812383516366 1103 82-83 -5.626968482211836 1103 84-85 -4.737489265908536 1103 86-87 -4.495673447033244 1103 88-89 -4.545284421767686 1103 90-91 -9.85176114983691 1103 92-93 -14.30896966769059 1103 94-95 -12.165745233353093 1103 96-97 -11.860294505632895 1103 98-99 -11.473822509794088 1103 100-101 -11.029307287476177 1104 1 0.1205674997467554 1104 2 0.10795193681486026 1104 3 0.10762981347838618 1104 4 0.21052854950197286 1104 5 0.009742839392984592 1104 6 0.09074025256398244 1104 7 0.09654094884219688 1104 8 0.09459564563585587 1104 9 0.12747894345368138 1104 10-11 0.20488222317435145 1104 12-13 0.10628883491143881 1104 14-15 0.164240719297716 1104 16-17 0.26827904189100593 1104 18-19 0.15083514885297689 1104 20-21 0.10334680362827697 1104 22-23 0.1652532130107076 1104 24-25 0.23566006715614662 1104 26-27 0.23937787797189003 1104 28-29 0.09155911097182923 1104 30-31 0.1349166346445898 1104 32-33 0.05236595910557895 1104 34-35 0.09143232430157866 1104 36-37 0.16695587697106617 1104 38-39 0.182332212545532 1104 40-41 0.1785859974619015 1104 42-43 0.22709924082730737 1104 44-45 0.08060411387566546 1104 46-47 0.027044706450162437 1104 48-49 0.0227858136256458 1104 50-51 0.06747347108058221 1104 52-53 0.1832337425401036 1104 54-55 0.0461052322381974 1104 56-57 0.5089182603044691 1104 58-59 -6.675479121555952 1104 60-61 -8.696515481001086 1104 62-63 -2.8491435814227373 1104 64-65 -0.6582433127687146 1104 66-67 -2.0468443827529086 1104 68-69 -4.322096621123624 1104 70-71 -5.111210862772333 1104 72-73 -7.886444644942966 1104 74-75 -8.678537829012708 1104 76-77 -9.274346506668348 1104 78-79 -12.424404327237752 1104 80-81 -8.9856600250356 1104 82-83 -5.548886782436451 1104 84-85 -4.685260990770704 1104 86-87 -4.383573267633576 1104 88-89 -4.621512871929578 1104 90-91 -9.895584623481204 1104 92-93 -14.941926425807331 1104 94-95 -12.179820996895579 1104 96-97 -11.844397853697421 1104 98-99 -11.167855374208777 1104 100-101 -10.748988014409464 1105 1 0.0986220822798316 1105 2 0.13263561901366572 1105 3 0.09398609009652503 1105 4 0.12420845790963853 1105 5 0.0468617268158269 1105 6 0.0325685997927323 1105 7 0.1145882120980275 1105 8 0.1943563514010549 1105 9 0.11732296880347093 1105 10-11 0.18067307460668758 1105 12-13 0.06164263940035397 1105 14-15 0.11611353311906925 1105 16-17 0.18614193487426434 1105 18-19 0.27500282347511273 1105 20-21 0.19650477058516458 1105 22-23 0.1725317949573082 1105 24-25 0.20006192779811016 1105 26-27 0.2140623902372667 1105 28-29 0.22046180116615943 1105 30-31 0.19896376303297103 1105 32-33 0.10114834694412167 1105 34-35 0.07758361855165674 1105 36-37 -0.01987021732479377 1105 38-39 -0.04195981780170399 1105 40-41 0.0029642959664712976 1105 42-43 -0.09675765834076344 1105 44-45 -0.12255251175241 1105 46-47 -0.16395662268474354 1105 48-49 -0.04864420595951913 1105 50-51 -0.012297468744286277 1105 52-53 0.019857836830965425 1105 54-55 -0.053457910338003956 1105 56-57 0.3893064758273681 1105 58-59 -6.506765263425642 1105 60-61 -8.666861499759417 1105 62-63 -2.8714361209754102 1105 64-65 -0.5931838704529788 1105 66-67 -1.6911540634005888 1105 68-69 -3.9990730940987866 1105 70-71 -4.582183691975635 1105 72-73 -6.228988215724797 1105 74-75 -6.800383888245964 1105 76-77 -8.604273836911288 1105 78-79 -11.76136166020008 1105 80-81 -8.148867199513976 1105 82-83 -4.47380134801854 1105 84-85 -3.4976454150196368 1105 86-87 -3.290573490796607 1105 88-89 -3.3580767753756504 1105 90-91 -8.736475480305298 1105 92-93 -11.822492591441899 1105 94-95 -11.601404571450175 1105 96-97 -11.684725477299107 1105 98-99 -11.0291920561139 1105 100-101 -10.499283349156212 1106 1 0.009008694981080367 1106 2 0.06780996071642065 1106 3 0.06577456428381367 1106 4 0.060255852857785897 1106 5 0.041151099850040396 1106 6 -0.0012086994388411654 1106 7 -4.849554574164472E-4 1106 8 0.0456759027293856 1106 9 0.03980398764135629 1106 10-11 0.055843918434433704 1106 12-13 0.013574853602413839 1106 14-15 0.1491603521131637 1106 16-17 -0.016695260119369948 1106 18-19 0.0528869739806197 1106 20-21 0.0021581009742490664 1106 22-23 0.06816056787318558 1106 24-25 0.12458324265939069 1106 26-27 0.2643394678633513 1106 28-29 0.13414190539779725 1106 30-31 0.10419415566629908 1106 32-33 0.08955385068687605 1106 34-35 0.14247006500947634 1106 36-37 0.1519297067411216 1106 38-39 0.04011623078227444 1106 40-41 0.14446969706924762 1106 42-43 0.1327884231741976 1106 44-45 -0.06065026855132771 1106 46-47 -0.05475875273963737 1106 48-49 -0.010179769943114536 1106 50-51 -0.024816526972905706 1106 52-53 -0.19328519259931198 1106 54-55 -0.39078258013955036 1106 56-57 0.3739935213906449 1106 58-59 -6.75157695853299 1106 60-61 -9.208672902014097 1106 62-63 -3.0274974948413167 1106 64-65 -0.68640145306275 1106 66-67 -1.9882733022478867 1106 68-69 -4.441380096800518 1106 70-71 -4.974297918355463 1106 72-73 -6.537865992359684 1106 74-75 -7.053201376236961 1106 76-77 -8.95396657753096 1106 78-79 -12.185726325622987 1106 80-81 -8.756150753556303 1106 82-83 -5.099701020658962 1106 84-85 -4.1582501730733235 1106 86-87 -3.9031692852310194 1106 88-89 -4.086170074900384 1106 90-91 -8.958055560410411 1106 92-93 -12.107510739814082 1106 94-95 -11.65505291485091 1106 96-97 -11.753235071133536 1106 98-99 -11.198106941050483 1106 100-101 -10.88318118667048 1107 1 0.05328963358027394 1107 2 -0.05549428948743795 1107 3 0.021301749631835065 1107 4 -0.02803801073861223 1107 5 -0.03575018204227831 1107 6 -0.09925382661212012 1107 7 -0.043220513955802176 1107 8 0.09131985274857612 1107 9 -0.008508076679397902 1107 10-11 0.04330982350559509 1107 12-13 0.025353073171793028 1107 14-15 0.054745037418364006 1107 16-17 0.04273587217091546 1107 18-19 0.07367951793806782 1107 20-21 0.08590154776273806 1107 22-23 0.18480741834799375 1107 24-25 0.1326374010503244 1107 26-27 0.2900398586713422 1107 28-29 0.10176733945647953 1107 30-31 0.04109003485698537 1107 32-33 0.055653862268592036 1107 34-35 0.06082554737659507 1107 36-37 -0.06713035832073189 1107 38-39 0.13876810114949478 1107 40-41 -0.02992689517434144 1107 42-43 0.15340527718323926 1107 44-45 0.07542686090749129 1107 46-47 -0.04873398611419333 1107 48-49 -0.10574634095461732 1107 50-51 -0.02069087754475163 1107 52-53 0.0019061436547502808 1107 54-55 -0.10138383420120078 1107 56-57 0.47655575028321806 1107 58-59 -6.560290912825 1107 60-61 -8.902685952728621 1107 62-63 -3.014978210983717 1107 64-65 -0.586334477162918 1107 66-67 -1.9826701600693042 1107 68-69 -4.3319518167218245 1107 70-71 -4.693136878088678 1107 72-73 -6.347541448395319 1107 74-75 -7.1103490499244675 1107 76-77 -9.31563950099127 1107 78-79 -12.325431263940573 1107 80-81 -8.73314444553285 1107 82-83 -4.932017850923142 1107 84-85 -3.9852433464659427 1107 86-87 -3.652305607273526 1107 88-89 -3.717942447043125 1107 90-91 -8.887813092566935 1107 92-93 -12.15218081279141 1107 94-95 -11.710563041930685 1107 96-97 -12.060227444649739 1107 98-99 -11.193689234583758 1107 100-101 -10.612240689147349 1108 1 -0.05256154602377805 1108 2 -0.07781680042036854 1108 3 -0.09295355146958073 1108 4 0.017329850173965156 1108 5 -0.0925193807593061 1108 6 -0.0023446340844159863 1108 7 -0.054615991512292794 1108 8 0.0724761502950031 1108 9 -0.054884726686680096 1108 10-11 -0.026547863377899716 1108 12-13 -0.08175460370044618 1108 14-15 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58-59 4.725136821722106 2311 60-61 4.413243171419683 2311 62-63 1.893463640706667 2311 64-65 1.6444126332319406 2311 66-67 -0.22332477254387584 2311 68-69 -3.1998383333363254 2311 70-71 -0.7158217745504825 2311 72-73 -1.134982354231525 2311 74-75 1.0459490985458935 2311 76-77 4.26214424313298 2311 78-79 5.289204589442836 2311 80-81 3.388261654346831 2311 82-83 1.9501731261797737 2311 84-85 1.527257561338292 2311 86-87 1.510485902876109 2311 88-89 1.586821897838636 2311 90-91 3.025715540139778 2311 92-93 5.002587409491486 2311 94-95 8.893590107232246 2311 96-97 9.655864682401292 2311 98-99 10.152225631879054 2311 100-101 9.467876084707193 2312 1 -0.30699335977083564 2312 2 -0.35330942962018597 2312 3 -0.3865866388334602 2312 4 -0.2604052779256065 2312 5 -0.2754964358177787 2312 6 -0.2172149298085415 2312 7 -0.3929338726457843 2312 8 -0.3593493807812891 2312 9 -0.3853498758997489 2312 10-11 -0.39185291663577004 2312 12-13 -0.41284423662616376 2312 14-15 -0.47275054252309445 2312 16-17 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82-83 1.670618688126492 2312 84-85 1.2668844072328689 2312 86-87 1.1130907226591553 2312 88-89 1.3124957644720077 2312 90-91 2.7575150935797765 2312 92-93 4.635652483490272 2312 94-95 8.57348716448293 2312 96-97 9.072374275464925 2312 98-99 9.475954411243633 2312 100-101 8.580317135760573 2313 1 -0.3023589816767007 2313 2 -0.2174844235879192 2313 3 -0.2512661798819913 2313 4 -0.2616213162658596 2313 5 -0.333124698441857 2313 6 -0.17931738681303955 2313 7 -0.30537099146894775 2313 8 -0.3061191978164146 2313 9 -0.265506498496201 2313 10-11 -0.3917013861041809 2313 12-13 -0.32112220346117937 2313 14-15 -0.415245421336941 2313 16-17 -0.4354932800290712 2313 18-19 -0.5176141774455161 2313 20-21 -0.42766263920236725 2313 22-23 -0.45461819511435664 2313 24-25 -0.46001869028589937 2313 26-27 -0.42028661783177057 2313 28-29 -0.5739981515388664 2313 30-31 -0.6163060891250822 2313 32-33 -0.40206587043226705 2313 34-35 -0.47080604153823913 2313 36-37 -0.5641202669664764 2313 38-39 -0.9496937023080818 2313 40-41 -1.0602347581339302 2313 42-43 -0.7654304384552049 2313 44-45 -0.7272977145612316 2313 46-47 -0.554058758179444 2313 48-49 -0.6598747780121599 2313 50-51 -0.3763396370927623 2313 52-53 -0.535985187159504 2313 54-55 -0.5286183341876765 2313 56-57 0.6204279444989496 2313 58-59 4.206066081232013 2313 60-61 3.884900689528809 2313 62-63 1.3328247956807253 2313 64-65 1.2956405785244023 2313 66-67 1.9500350623643428 2313 68-69 0.25732566395670275 2313 70-71 2.6036448185935317 2313 72-73 8.258320266683995 2313 74-75 9.885219519880831 2313 76-77 8.794689680968741 2313 78-79 6.430400168057766 2313 80-81 3.623143775809595 2313 82-83 2.1615819037820962 2313 84-85 1.5722489474909658 2313 86-87 1.4215856513952083 2313 88-89 1.5596787709535747 2313 90-91 2.99799967177875 2313 92-93 4.908111701209329 2313 94-95 8.677226437449225 2313 96-97 9.416450290341738 2313 98-99 9.802779675207288 2313 100-101 8.949441816466475 2314 1 -0.36469667298975494 2314 2 -0.3578530630362273 2314 3 -0.3297348303168057 2314 4 -0.24818742543789796 2314 5 -0.48908971065377926 2314 6 -0.3373120287797917 2314 7 -0.3755943555363217 2314 8 -0.3989069470198885 2314 9 -0.39247782372206075 2314 10-11 -0.29174408134702645 2314 12-13 -0.5473251495343519 2314 14-15 -0.6469789056642767 2314 16-17 -0.5676024689715717 2314 18-19 -0.5836061826514296 2314 20-21 -0.5429099453962252 2314 22-23 -0.6276509940528925 2314 24-25 -0.5135561246838023 2314 26-27 -0.6430285786691101 2314 28-29 -0.8251701748302551 2314 30-31 -0.7326213510686799 2314 32-33 -0.7771423280454925 2314 34-35 -0.898820171871975 2314 36-37 -0.7609002073013116 2314 38-39 -0.6276121486474722 2314 40-41 -0.7245167225207538 2314 42-43 -0.7631295603800368 2314 44-45 -0.7688848417595864 2314 46-47 -0.612408010674649 2314 48-49 -0.7527577210429115 2314 50-51 -0.5249726287176628 2314 52-53 -0.6451329218269919 2314 54-55 -0.6646482174772004 2314 56-57 0.7814581742859019 2314 58-59 4.083001286214813 2314 60-61 3.8193660271102594 2314 62-63 1.4109965400839464 2314 64-65 1.3744668142947702 2314 66-67 1.8307219385633537 2314 68-69 -0.07486100518896777 2314 70-71 2.37688277780825 2314 72-73 8.310504062436443 2314 74-75 9.986261108022166 2314 76-77 9.016251860352991 2314 78-79 6.489664072323066 2314 80-81 3.65995217963966 2314 82-83 2.1669440273887126 2314 84-85 1.7352176021513586 2314 86-87 1.6641872554247215 2314 88-89 1.8893950318238772 2314 90-91 3.2620558888720588 2314 92-93 5.271854752245861 2314 94-95 8.995203298182613 2314 96-97 9.59866107908818 2314 98-99 9.737661756220076 2314 100-101 8.931049158831279 2315 1 -0.7787581629350235 2315 2 -0.5441838041687248 2315 3 -0.5658195813246216 2315 4 -0.4062705891410161 2315 5 -0.8430983664224456 2315 6 -0.612532413761798 2315 7 -0.7290308382677253 2315 8 -0.8456967056597051 2315 9 -1.1720847839653246 2315 10-11 -0.8643710023860578 2315 12-13 -0.6255996478430532 2315 14-15 -0.8213140848037241 2315 16-17 -0.9511742210457044 2315 18-19 -0.9283934300309014 2315 20-21 -1.0339349155008648 2315 22-23 -0.9810798545857509 2315 24-25 -1.0368655337507207 2315 26-27 -1.0163339189338458 2315 28-29 -0.9480939514655731 2315 30-31 -1.0538303072535982 2315 32-33 -0.9503139241331482 2315 34-35 -0.9298077844926809 2315 36-37 -1.0149042389361895 2315 38-39 -0.9933561590694069 2315 40-41 -1.0124428043266889 2315 42-43 -0.904908696215827 2315 44-45 -0.8116143423466013 2315 46-47 -0.9149681692396072 2315 48-49 -0.7474436694037507 2315 50-51 -0.700698024278914 2315 52-53 -0.6750680177175141 2315 54-55 -0.745208722017388 2315 56-57 0.6355125269496398 2315 58-59 4.021668866383823 2315 60-61 3.684478447642487 2315 62-63 1.3596757328539226 2315 64-65 1.227081188469075 2315 66-67 1.9332702718317165 2315 68-69 5.818119879135605 2315 70-71 4.994542627833871 2315 72-73 8.923383451017074 2315 74-75 9.99798237975623 2315 76-77 8.778202905076931 2315 78-79 6.233956152648261 2315 80-81 3.4059873478829985 2315 82-83 1.7956255402107395 2315 84-85 1.3077648530675283 2315 86-87 1.154374110355338 2315 88-89 1.3539964892027534 2315 90-91 2.6706814082976678 2315 92-93 4.564843062769587 2315 94-95 8.473214925011678 2315 96-97 9.250116233011946 2315 98-99 9.359860843254912 2315 100-101 8.433736491964112 2316 1 -0.4936002123395937 2316 2 -0.48550192133703973 2316 3 -0.626925392119503 2316 4 -0.376702011446703 2316 5 -0.6786738858514809 2316 6 -0.6632985067053667 2316 7 -0.7037550870699576 2316 8 -0.6418280294505934 2316 9 -0.880173832090243 2316 10-11 -0.697072867683346 2316 12-13 -0.6305564004654585 2316 14-15 -0.8228106331924891 2316 16-17 -1.0321632873955906 2316 18-19 -0.9251096165717243 2316 20-21 -0.7890747184697133 2316 22-23 -0.879114425458944 2316 24-25 -2.3612299274058657 2316 26-27 -1.6577849853754358 2316 28-29 -1.2824615690566148 2316 30-31 -0.8358744294763127 2316 32-33 -0.6431132980630707 2316 34-35 -0.7560807216404584 2316 36-37 -0.6459075110709875 2316 38-39 -1.0575149051419146 2316 40-41 -0.7002028547535204 2316 42-43 -0.8105990407270056 2316 44-45 -0.8211851432833654 2316 46-47 -0.8867822046175959 2316 48-49 -1.0808435203387035 2316 50-51 -1.0794784640887656 2316 52-53 -0.9091190849559112 2316 54-55 -0.8593842517736405 2316 56-57 0.2551604672123773 2316 58-59 3.741938213065364 2316 60-61 3.7146994290360738 2316 62-63 1.1953951459085452 2316 64-65 0.8872213964805162 2316 66-67 5.975543906730778 2316 68-69 8.796067790424324 2316 70-71 5.186188431096598 2316 72-73 9.100891744625372 2316 74-75 10.190089314436882 2316 76-77 8.75123640377857 2316 78-79 6.45758188454694 2316 80-81 3.3348196854833816 2316 82-83 2.107496615896835 2316 84-85 1.6377479334582112 2316 86-87 1.6528271367031024 2316 88-89 1.7512143348558276 2316 90-91 3.0386899416115014 2316 92-93 4.699118040050873 2316 94-95 8.534946891808655 2316 96-97 9.300624067363666 2316 98-99 9.632397362121266 2316 100-101 8.760636483472666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 18.0 4 65.0 5 163.0 6 397.0 7 997.0 8 2372.0 9 4125.0 10 6627.0 11 9947.0 12 14067.0 13 19027.0 14 25115.0 15 33873.0 16 45306.0 17 57245.0 18 64023.0 19 68055.0 20 71410.0 21 71974.0 22 69800.0 23 67216.0 24 67811.0 25 71828.0 26 92578.0 27 132686.0 28 185348.0 29 241342.0 30 276656.0 31 289101.0 32 293439.0 33 296897.0 34 291977.0 35 272270.0 36 214953.0 37 121128.0 38 32371.0 39 998.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.094316373976646 20.253547639898464 14.812884130738544 23.839251855386344 2 15.860957832757148 21.382764475731097 38.7204511199006 24.035826571611157 3 19.377104616707133 24.633586765003088 30.345249650594486 25.644058967695287 4 13.47887903245318 17.364949198953095 36.97217505902713 32.18399670956659 5 13.354757453440563 37.70417283300807 34.85557944241463 14.085490271136738 6 32.059115117699946 37.06054827874085 17.059739901907456 13.820596701651736 7 27.822151505102877 32.65379431660175 21.22135992246418 18.3026942558312 8 25.83958734082239 35.847802041094 19.819344907540504 18.49326571054311 9 25.70545782139721 16.61113823093662 20.235368458804828 37.44803548886134 10-11 24.681879702779362 26.796267805983977 27.846075460795884 20.675777030440777 12-13 25.42588483767717 23.891677565990125 28.24677681269151 22.435660783641197 14-15 22.069985608594767 25.597601963789216 26.268854562553656 26.063557865062357 16-17 21.894077077548626 28.726593732521348 27.004552809890658 22.374776380039364 18-19 21.847163319059945 27.41381280756488 29.0697619807004 21.669261892674776 20-21 22.411651436077783 26.65125817805059 29.22898257009605 21.70810781577558 22-23 22.028967768838513 26.611693278094283 29.120492198741037 22.238846754326165 24-25 21.979227004325097 26.772301154355766 29.231487398294725 22.01698444302441 26-27 21.831776993784747 27.02171503195865 29.01352508950856 22.132982884748042 28-29 21.76869266983733 27.348673134654 28.948762057738236 21.933872137770436 30-31 21.99970850643136 27.056182538738188 28.877376853930407 22.066732100900044 32-33 21.741777591053637 26.934914681868744 28.9178909052819 22.40541682179572 34-35 22.21356910792654 27.112754633543457 28.76975486068771 21.9039213978423 36-37 22.264106785897216 26.850689534119958 28.861025214538966 22.024178465443857 38-39 21.865227279456818 27.10890042160072 28.87246008914885 22.15341220979362 40-41 22.2431283561012 26.965828270116678 28.830795556137645 21.96024781764448 42-43 21.97666706384847 26.971605487144906 28.736274272399235 22.315453176607384 44-45 21.967278787159223 27.071775462620902 28.819645824845104 22.14129992537477 46-47 22.1909024645802 27.212199899776774 28.364952052298303 22.23194558334472 48-49 21.960015443505355 27.308396893532066 28.353680759055123 22.37790690390746 50-51 21.995200606095374 27.40219886095182 28.362547417040908 22.2400531159119 52-53 22.29178111071427 27.31567966134194 28.15062357444651 22.24191565349728 54-55 21.920781635995226 27.34763742008309 28.31584288991234 22.415738054009353 56-57 22.142625633652095 27.158764288974147 28.433266239780252 22.265343837593505 58-59 21.14807598293867 26.713141762666393 29.855330345607616 22.28345190878732 60-61 22.09122289094709 27.03947443554926 28.648630129164143 22.220672544339504 62-63 21.77543758876341 27.266511755730495 28.39666327137797 22.561387384128125 64-65 21.161905033387256 27.228323225700706 28.844138166943413 22.76563357396863 66-67 22.153313741980206 26.25602197372634 29.5622288181125 22.028435466180955 68-69 23.47047926355583 24.937750808694627 30.408205206216216 21.183564721533326 70-71 22.453078868064473 25.435479288008988 30.275258322471238 21.836183521455304 72-73 22.198438583667407 25.692854753422456 30.124306325982236 21.984400336927898 74-75 22.522918306528148 25.953678385487255 29.390856963601763 22.132546344382835 76-77 20.56311710470436 26.586938323097094 29.882255317881285 22.967689254317257 78-79 21.271107833426946 26.99976131980145 28.612724941794905 23.1164059049767 80-81 21.58703976352094 27.19401003214299 27.80366659242302 23.415283611913047 82-83 21.636927948209173 27.1993923504124 27.695519964795718 23.46815973658271 84-85 21.590241643372472 27.126880524943754 27.66046593511441 23.622411896569368 86-87 21.551257048021117 27.100963912110892 27.602738468514488 23.745040571353506 88-89 21.53097967687427 27.124475320251584 27.422564150295976 23.921980852578173 90-91 21.473701237061082 26.90785794173473 27.787852019383436 23.830588801820753 92-93 21.248614600450342 26.84488743013837 27.85600455759741 24.050493411813882 94-95 20.62673982053148 26.155323016375704 29.424956467990764 23.792980695102052 96-97 20.342326039447553 25.99128804417471 29.573311934232073 24.093073982145665 98-99 20.498584932852058 26.203719929553564 28.849443382004015 24.448251755590366 100-101 20.084582849452616 26.49117068625253 28.714871403117613 24.70937506117724 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 312.0 1 243.0 2 174.0 3 200.5 4 233.0 5 282.5 6 427.5 7 674.5 8 953.5 9 1210.0 10 1452.0 11 1629.5 12 1839.0 13 2136.5 14 2387.5 15 2734.0 16 3162.0 17 3535.5 18 4007.5 19 4674.5 20 5524.0 21 6682.5 22 8284.0 23 10363.5 24 13007.5 25 16533.0 26 20967.0 27 26116.5 28 31551.0 29 37361.5 30 43566.0 31 50583.5 32 58779.5 33 66286.0 34 73152.0 35 80460.0 36 87876.5 37 94671.0 38 101095.0 39 107242.5 40 113015.0 41 118321.5 42 123387.0 43 128566.5 44 132931.5 45 136019.5 46 138791.0 47 140868.0 48 140734.5 49 138884.5 50 135335.5 51 130492.5 52 125360.0 53 120056.0 54 113317.5 55 103975.5 56 93372.0 57 82958.0 58 71586.0 59 59425.5 60 48119.5 61 38086.5 62 29350.0 63 21693.5 64 15773.0 65 11562.0 66 8319.0 67 6036.0 68 4349.0 69 3009.0 70 2143.0 71 1547.5 72 1108.0 73 822.0 74 604.0 75 388.0 76 256.0 77 177.5 78 103.0 79 63.5 80 41.5 81 23.5 82 11.0 83 8.5 84 6.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02103486407749028 2 0.005123512224557848 3 0.0029887154643254113 4 0.0 5 0.06316152014607702 6 5.6927913606198315E-5 7 0.0 8 2.8463956803099158E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9924769762169408E-4 16-17 0.0 18-19 9.535425529038217E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.962384881084704E-5 28-29 0.0018928531274060938 30-31 0.00777066020724607 32-33 3.131035248340907E-4 34-35 8.254547472898756E-4 36-37 0.026072984431638826 38-39 0.02524752968434895 40-41 0.027183078746959695 42-43 0.027595806120604634 44-45 0.028407028889492956 46-47 0.03002947442726961 48-49 0.030143330254482008 50-51 0.025517937273978393 52-53 0.021732231019166205 54-55 0.01658025483780526 56-57 0.14738636832644741 58-59 0.7576109062496887 60-61 0.3044220180091455 62-63 3.842634168418386E-4 64-65 0.14630473796792964 66-67 1.4073149522588286 68-69 2.701158340722102 70-71 2.179499404391704 72-73 1.8501429602230435 74-75 1.5502182473887878 76-77 1.1065505526988813 78-79 0.4215512002538985 80-81 2.1347967602324368E-4 82-83 1.7078374081859493E-4 84-85 1.4231978401549579E-5 86-87 0.0023767403930587796 88-89 1.4231978401549579E-5 90-91 0.22345629288272992 92-93 0.17374399232611726 94-95 1.2912531683941917 96-97 1.2675996202908162 98-99 0.9994122192920161 100-101 0.4030638603102856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3513215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.97496084296267 #Duplication Level Percentage of deduplicated Percentage of total 1 71.15766424881284 36.98416813008731 2 15.112188362653203 15.709107968007526 3 5.815443742928555 9.067723824694918 4 2.7830399571097035 5.7859357118070935 5 1.5221216825870214 3.9556107425342444 6 0.9029212439271893 2.815757777843688 7 0.5965185222562853 2.170281878546067 8 0.38741622226661604 1.6108754385788713 9 0.27825727462715194 1.301616985771416 >10 1.3269183766568111 11.968828699748524 >50 0.07707844956720947 2.7279139875524248 >100 0.036376543675295636 3.3666352684229297 >500 0.0027401168528363583 0.9856143143833482 >1k 0.0011508490693660964 0.9206434609993872 >5k 1.644070099094423E-4 0.6292858110221973 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9207 0.26206765028613394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7382 0.21012092912047797 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5504 0.15666561824425776 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.8463956803099154E-5 0.0 0.0 0.0 0.0 8 2.8463956803099154E-5 0.0 0.0 0.0 0.0 9 2.8463956803099154E-5 0.0 0.0 0.0 0.0 10-11 2.8463956803099154E-5 0.0 0.0 0.0 2.8463956803099154E-5 12-13 2.8463956803099154E-5 0.0 0.0 0.0 2.8463956803099154E-5 14-15 5.692791360619831E-5 0.0 0.0 0.0 2.8463956803099154E-5 16-17 5.692791360619831E-5 0.0 0.0 2.8463956803099154E-5 8.539187040929746E-5 18-19 9.962384881084704E-5 0.0 0.0 8.539187040929746E-5 8.539187040929746E-5 20-21 1.4231978401549577E-4 0.0 0.0 1.4231978401549577E-4 8.539187040929746E-5 22-23 1.7078374081859493E-4 0.0 0.0 2.2771165442479323E-4 1.1385582721239662E-4 24-25 1.7078374081859493E-4 0.0 0.0 7.115989200774789E-4 1.4231978401549577E-4 26-27 1.7078374081859493E-4 0.0 0.0 0.0016936054297843998 1.7078374081859493E-4 28-29 2.419436328263428E-4 0.0 0.0 0.0052231360733686946 1.7078374081859493E-4 30-31 2.561756112278924E-4 0.0 0.0 0.017391477606693583 1.7078374081859493E-4 32-33 2.561756112278924E-4 0.0 0.0 0.04816101491084377 1.9924769762169408E-4 34-35 2.7040758962944197E-4 0.0 0.0 0.10054892740694776 1.9924769762169408E-4 36-37 2.8463956803099154E-4 0.0 0.0 0.19137741356563717 1.9924769762169408E-4 38-39 2.8463956803099154E-4 0.0 0.0 0.3337398935163376 1.9924769762169408E-4 40-41 2.8463956803099154E-4 0.0 0.0 0.4871748526634436 1.9924769762169408E-4 42-43 3.4156748163718985E-4 0.0 0.0 0.644039718605323 2.1347967602324366E-4 44-45 3.70031438440289E-4 0.0 0.0 0.8367407061623042 2.2771165442479323E-4 46-47 4.1272737364493774E-4 0.0 0.0 1.0614209491875677 2.419436328263428E-4 48-49 4.269593520464873E-4 0.0 0.0 1.2780174284807506 2.988715464325411E-4 50-51 4.269593520464873E-4 0.0 0.0 1.495069331082783 3.131035248340907E-4 52-53 4.269593520464873E-4 0.0 0.0 1.7059018591233386 3.131035248340907E-4 54-55 4.269593520464873E-4 0.0 0.0 1.936388749336434 3.4156748163718985E-4 56-57 4.269593520464873E-4 1.4231978401549577E-5 0.0 2.1357503027853406 3.842634168418386E-4 58-59 4.269593520464873E-4 5.692791360619831E-5 0.0 2.271637232563336 3.9849539524338816E-4 60-61 4.269593520464873E-4 5.692791360619831E-5 0.0 2.371204153460577 4.269593520464873E-4 62-63 5.692791360619831E-4 5.692791360619831E-5 0.0 2.4579053658828167 4.269593520464873E-4 64-65 5.692791360619831E-4 5.692791360619831E-5 0.0 2.5457024406419757 4.411913304480369E-4 66-67 5.692791360619831E-4 5.692791360619831E-5 0.0 2.624376817245742 4.5542330884958647E-4 68-69 5.692791360619831E-4 5.692791360619831E-5 2.8463956803099154E-5 2.714892199879597 4.5542330884958647E-4 70-71 5.692791360619831E-4 5.692791360619831E-5 2.8463956803099154E-5 2.873023711899215 4.6965528725113605E-4 72-73 5.835111144635327E-4 7.115989200774789E-5 2.8463956803099154E-5 3.0819491548339624 5.123512224557848E-4 74-75 6.119750712666318E-4 8.539187040929746E-5 2.8463956803099154E-5 3.3249317220836185 5.123512224557848E-4 76-77 6.262070496681814E-4 8.539187040929746E-5 2.8463956803099154E-5 3.6772016514787738 5.123512224557848E-4 78-79 6.262070496681814E-4 8.539187040929746E-5 2.8463956803099154E-5 4.178138827256516 5.123512224557848E-4 80-81 6.831349632743797E-4 8.539187040929746E-5 2.8463956803099154E-5 4.724746421724831 5.265832008573344E-4 82-83 6.831349632743797E-4 8.539187040929746E-5 2.8463956803099154E-5 5.246163414422402 5.408151792588839E-4 84-85 6.973669416759293E-4 8.539187040929746E-5 2.8463956803099154E-5 5.666234488922539 5.408151792588839E-4 86-87 7.115989200774789E-4 8.539187040929746E-5 2.8463956803099154E-5 6.0394396585463745 5.408151792588839E-4 88-89 7.115989200774789E-4 8.539187040929746E-5 2.8463956803099154E-5 6.418608027120458 5.408151792588839E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6310 0.0 40.732704 1 GTATCAA 10390 0.0 35.26806 1 ATCAACG 12940 0.0 27.909962 3 TCAACGC 12965 0.0 27.85535 4 CAACGCA 13320 0.0 27.148357 5 TATCAAC 13450 0.0 27.133646 2 AACGCAG 13760 0.0 26.366507 6 GTACATG 10435 0.0 23.501034 1 CGCAGAG 15860 0.0 22.786556 8 ACGCAGA 15895 0.0 22.765701 7 TACATGG 10405 0.0 22.657784 2 ACATGGG 10610 0.0 21.596611 3 GCAGAGT 17660 0.0 19.770336 9 AGAGTAC 14980 0.0 19.595789 10-11 GTAAGGT 1595 0.0 19.213436 4 GAGTACT 10350 0.0 19.166267 12-13 TAAGGTG 1845 0.0 19.165375 5 AGTACTT 11110 0.0 18.491333 12-13 GGTAAGG 1620 0.0 18.335396 3 GTACTTT 11075 0.0 17.699116 14-15 >>END_MODULE