FastQCFastQC Report
Fri 27 May 2016
SRR939726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3707312
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.24853586641750142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65290.17611142520510817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45680.12321595808499528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60200.038.2160381
GTATCAA106050.038.1079671
ATCAACG130250.030.4509413
TCAACGC131450.030.2449514
TATCAAC135550.029.4677732
CAACGCA133750.029.4094475
AACGCAG139400.028.4544946
ACGCAGA158850.024.6742447
GTACATG113950.024.6486571
TACATGG111550.024.420142
CGCAGAG161500.024.12378
ACATGGG113850.023.2662413
GCAGAGT175700.020.674349
AGAGTAC146050.020.5055410-11
CATGGGG64000.019.5550274
GAGTACT97650.019.08057412-13
AGTACTT107750.018.84221812-13
GTACTTT104550.017.78027214-15
CGATACG800.00910094517.69325492-93
GAGTACA87550.017.6258891
Produced by FastQC (version 0.11.5)