##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939726_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3707312 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.510671073813047 31.0 31.0 33.0 27.0 34.0 2 30.624306775367167 31.0 31.0 34.0 27.0 34.0 3 30.60195985662928 31.0 31.0 34.0 27.0 34.0 4 34.27338864384762 35.0 35.0 37.0 30.0 37.0 5 34.14875197987113 35.0 35.0 37.0 30.0 37.0 6 34.14334941326762 36.0 35.0 37.0 30.0 37.0 7 34.13580567268145 36.0 35.0 37.0 30.0 37.0 8 33.99080330978347 36.0 35.0 37.0 29.0 37.0 9 35.36856299119146 38.0 35.0 39.0 29.0 39.0 10-11 35.19421146642095 37.0 35.0 39.0 28.5 39.0 12-13 35.113749395788645 37.0 35.0 39.0 29.0 39.0 14-15 35.81601170875287 38.0 34.5 40.0 27.0 41.0 16-17 35.62548889869534 38.0 34.0 40.0 27.0 41.0 18-19 35.64501126422594 38.0 34.0 40.0 27.0 41.0 20-21 35.75484340136465 38.0 34.0 40.0 27.0 41.0 22-23 35.67817869119189 38.0 34.0 40.0 27.0 41.0 24-25 35.54415314923589 38.0 34.0 40.0 27.0 41.0 26-27 35.4678338375621 38.0 34.0 40.0 27.0 41.0 28-29 35.359271353476586 38.0 34.0 40.0 26.5 41.0 30-31 35.19492707924232 38.0 34.0 40.0 25.5 41.0 32-33 34.940580533820736 38.0 34.0 40.0 25.0 41.0 34-35 34.68959558839396 38.0 33.5 40.0 24.5 41.0 36-37 34.49177139663454 38.0 33.0 40.0 24.0 41.0 38-39 34.234698752087766 38.0 33.0 40.0 24.0 41.0 40-41 33.96523680769248 38.0 33.0 40.0 23.0 41.0 42-43 33.679948841640524 38.0 33.0 40.0 21.5 41.0 44-45 33.321507874168674 37.5 32.0 40.0 19.0 41.0 46-47 32.97194355371223 37.0 31.0 40.0 17.0 41.0 48-49 32.664732560949815 37.0 31.0 40.0 15.0 41.0 50-51 31.93891895259962 36.0 30.5 38.5 13.5 40.0 52-53 32.0626971779014 36.5 31.0 39.0 13.0 40.0 54-55 32.170457193783534 37.0 31.0 39.0 10.5 40.0 56-57 31.96479120181954 36.0 31.0 39.0 8.5 40.0 58-59 31.453701630723288 36.0 30.0 39.0 8.0 40.0 60-61 30.987415410410563 35.0 29.0 39.0 7.0 40.0 62-63 30.571009804408153 35.0 28.0 38.0 2.0 40.0 64-65 30.067773497347943 34.0 28.0 38.0 2.0 40.0 66-67 29.543878826492076 34.0 27.5 38.0 2.0 39.5 68-69 29.012208306179787 34.0 26.5 37.0 2.0 39.0 70-71 28.4736617797477 33.5 26.0 36.5 2.0 39.0 72-73 28.00240052631125 33.0 26.0 36.0 2.0 39.0 74-75 27.416943596870183 32.5 25.0 35.5 2.0 38.0 76-77 26.764900688153574 32.0 23.5 35.0 2.0 37.5 78-79 26.121893841144203 31.5 22.0 35.0 2.0 37.0 80-81 25.44898662427117 31.0 20.0 34.5 2.0 36.0 82-83 24.693663630144968 31.0 18.0 34.0 2.0 36.0 84-85 23.950268685236097 30.0 11.5 34.0 2.0 35.0 86-87 23.17409190270471 29.5 6.0 34.0 2.0 35.0 88-89 22.31364800157095 29.0 2.0 34.0 2.0 35.0 90-91 21.356340793545296 29.0 2.0 33.5 2.0 35.0 92-93 20.373164033671834 27.5 2.0 33.0 2.0 34.0 94-95 18.970789617922634 25.5 2.0 32.5 2.0 34.0 96-97 16.23674848515582 11.0 2.0 31.5 2.0 34.0 98-99 14.779016306153892 2.0 2.0 31.0 2.0 34.0 100-101 13.443443524580612 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8046783999208245 1101 2 0.6907285790355324 1101 3 0.8240092467726008 1101 4 0.8696039688020534 1101 5 0.8785672681489771 1101 6 0.9993586644483301 1101 7 0.8532512297122992 1101 8 0.7026274348793606 1101 9 0.8452873419224431 1101 10-11 0.9046931342452211 1101 12-13 0.7339401815539617 1101 14-15 0.8827814033710268 1101 16-17 0.888273516530063 1101 18-19 0.8676823886204872 1101 20-21 0.8901459824132516 1101 22-23 0.8708894554135966 1101 24-25 0.9384416118199326 1101 26-27 0.9435261811196369 1101 28-29 1.154135227335864 1101 30-31 1.0960684247274841 1101 32-33 1.0504368535341229 1101 34-35 0.9538255667881828 1101 36-37 0.9364551666974705 1101 38-39 0.9515861137091051 1101 40-41 1.0131934339210815 1101 42-43 1.0022939636635542 1101 44-45 1.0193373583674727 1101 46-47 0.9293142381641673 1101 48-49 0.7866106114351226 1101 50-51 0.8502107855954826 1101 52-53 1.066443348076099 1101 54-55 0.936425877027915 1101 56-57 0.5777238582008124 1101 58-59 0.466603168318354 1101 60-61 0.5336275751129023 1101 62-63 0.519151680819359 1101 64-65 0.5643699501888655 1101 66-67 0.40768124222131874 1101 68-69 0.4986844303351816 1101 70-71 0.558753259777756 1101 72-73 0.24292056357278113 1101 74-75 0.15769163853432033 1101 76-77 -0.023640383867913783 1101 78-79 0.01335349692088883 1101 80-81 -0.2527235274460935 1101 82-83 -0.30662498304022634 1101 84-85 -0.2511710735185382 1101 86-87 -0.4939052927314158 1101 88-89 -0.39355036692789724 1101 90-91 -0.7079643169241407 1101 92-93 -0.7732141964870323 1101 94-95 -0.24721727766644364 1101 96-97 1.4060569125453029 1101 98-99 1.4237085364945656 1101 100-101 0.4896542750288475 1102 1 0.693554023484527 1102 2 0.6938794674386486 1102 3 0.7120135626741764 1102 4 0.8292878430243462 1102 5 0.9052922275122413 1102 6 0.8604723912573675 1102 7 0.6960635720697894 1102 8 0.6844188142863459 1102 9 0.8675957887583863 1102 10-11 0.7286371396260805 1102 12-13 0.7195792993164147 1102 14-15 0.8172099667923547 1102 16-17 0.9131340904889171 1102 18-19 0.8007297234111377 1102 20-21 0.7067942924855615 1102 22-23 0.6482852011677593 1102 24-25 0.7299786613126713 1102 26-27 1.1261535562667646 1102 28-29 1.0145251998710378 1102 30-31 0.9414487285221185 1102 32-33 0.9960096913795411 1102 34-35 0.9487462550904056 1102 36-37 0.7845190364357109 1102 38-39 0.8921068244316004 1102 40-41 0.9642041676243451 1102 42-43 0.8212958413609925 1102 44-45 0.7297435855968857 1102 46-47 0.8326453294707079 1102 48-49 0.6273894354920628 1102 50-51 0.7698638319493831 1102 52-53 0.775051329691486 1102 54-55 0.7276294250353175 1102 56-57 0.6577059219864694 1102 58-59 0.4506358738841847 1102 60-61 0.6556201764244918 1102 62-63 0.33638053570417625 1102 64-65 0.3116837218384383 1102 66-67 0.2620204048243089 1102 68-69 0.009947672305923305 1102 70-71 0.3279487925393738 1102 72-73 0.1589411061432635 1102 74-75 0.010092568975437644 1102 76-77 -0.023576345977662783 1102 78-79 -0.1391759296807571 1102 80-81 -0.24472747901831582 1102 82-83 -0.33356335532877424 1102 84-85 -0.5295257061063978 1102 86-87 -0.6590671390404808 1102 88-89 -0.638135031464639 1102 90-91 -0.7416984125465831 1102 92-93 -0.7884909466691958 1102 94-95 -0.5073125638051152 1102 96-97 0.6012877011782258 1102 98-99 0.4528854324084719 1102 100-101 0.07887643185862103 1103 1 0.7180420943024295 1103 2 0.7080354072489392 1103 3 0.7607670216551341 1103 4 0.7772889994597705 1103 5 0.7991328321627691 1103 6 0.7753826986764523 1103 7 0.7095339108778305 1103 8 0.7529929569409148 1103 9 0.9200960985178739 1103 10-11 0.8951910997290611 1103 12-13 0.7251341473965951 1103 14-15 0.907327881904493 1103 16-17 0.8340592044115809 1103 18-19 0.7784548153064534 1103 20-21 0.7499488487034611 1103 22-23 0.9780396995638938 1103 24-25 0.8545613464455855 1103 26-27 1.0843510313827593 1103 28-29 1.1617879929379242 1103 30-31 1.1473181889899209 1103 32-33 1.071775072659051 1103 34-35 1.0041032356925825 1103 36-37 0.8329581583708148 1103 38-39 0.8903334857749883 1103 40-41 0.927482145789746 1103 42-43 0.8584747240240986 1103 44-45 0.7467166502884268 1103 46-47 0.6859492930154616 1103 48-49 0.585598130785101 1103 50-51 0.7342093445669171 1103 52-53 0.8514889578974945 1103 54-55 0.8151290808581066 1103 56-57 0.7357502382440231 1103 58-59 0.4502828513199262 1103 60-61 0.7758803055597774 1103 62-63 0.6129646457307452 1103 64-65 0.5543919834681859 1103 66-67 0.32556611844134054 1103 68-69 0.28431875729015843 1103 70-71 0.37079510364803525 1103 72-73 0.15539012661492535 1103 74-75 0.08834924257153887 1103 76-77 -0.09932283192835101 1103 78-79 -0.12391074114048095 1103 80-81 -0.34121725007163306 1103 82-83 -0.27317915030929285 1103 84-85 -0.4917608617433302 1103 86-87 -0.4750226093930543 1103 88-89 -0.21509553368802514 1103 90-91 -0.5560946568848699 1103 92-93 -0.6786933572863738 1103 94-95 -0.41122317410014375 1103 96-97 1.1014369019987456 1103 98-99 1.2939314557849855 1103 100-101 1.0217266665874796 1104 1 0.4959596448678276 1104 2 0.4844595170390882 1104 3 0.572025962784398 1104 4 0.7356862998995197 1104 5 0.7841569285318073 1104 6 0.8488521101882043 1104 7 0.8161972177021966 1104 8 0.8090509443497211 1104 9 0.962168673850698 1104 10-11 0.8009876494083912 1104 12-13 0.7744532315291437 1104 14-15 0.8869164975170776 1104 16-17 0.9324197063610669 1104 18-19 0.9631911452904447 1104 20-21 0.8695657171204445 1104 22-23 0.7624330380374218 1104 24-25 0.9578479090393159 1104 26-27 1.1577752151259588 1104 28-29 1.037029333241108 1104 30-31 1.0631227434022534 1104 32-33 1.0881053100072577 1104 34-35 1.057482821637663 1104 36-37 1.0007170248622757 1104 38-39 0.8479844766773965 1104 40-41 0.8828615112451743 1104 42-43 0.8498264282995436 1104 44-45 0.9090374682091706 1104 46-47 0.7717828329151857 1104 48-49 0.32084079950419664 1104 50-51 0.6025050554269278 1104 52-53 0.6478537546019254 1104 54-55 0.5084195876145401 1104 56-57 0.48169600189829254 1104 58-59 0.36221688793764173 1104 60-61 0.6340357178959692 1104 62-63 0.4005180391486505 1104 64-65 0.5019634132134705 1104 66-67 0.4262157754498297 1104 68-69 0.14315861195871804 1104 70-71 0.33411233621531977 1104 72-73 0.49775276224687204 1104 74-75 0.49486385583132275 1104 76-77 0.23074784801517367 1104 78-79 0.2942485674258819 1104 80-81 0.28657108808005916 1104 82-83 0.40459936167315647 1104 84-85 0.15869418918155986 1104 86-87 0.2237924641559914 1104 88-89 0.21754559680761076 1104 90-91 0.020426139671659627 1104 92-93 0.2158070693131684 1104 94-95 0.6428637933180568 1104 96-97 2.080093846121734 1104 98-99 2.080501855665567 1104 100-101 1.7281289807701672 1105 1 0.5847870867377445 1105 2 0.6554710109805555 1105 3 0.6313677534551374 1105 4 0.6491938823788814 1105 5 0.7023440420420997 1105 6 0.8185536876419235 1105 7 0.642430767548781 1105 8 0.6539033924847502 1105 9 0.9257775140937099 1105 10-11 0.8431860604404093 1105 12-13 0.5044136766569167 1105 14-15 0.652890841034889 1105 16-17 0.669034716893222 1105 18-19 0.7588690643248697 1105 20-21 0.6332321286672098 1105 22-23 0.5215440355110559 1105 24-25 0.6541045890038291 1105 26-27 0.8277597386048896 1105 28-29 0.7191212712266619 1105 30-31 0.8225503830465257 1105 32-33 0.7745328751474929 1105 34-35 0.8556581499634177 1105 36-37 0.7631666499714882 1105 38-39 0.799861301179746 1105 40-41 0.6439922455163583 1105 42-43 0.5425655016047486 1105 44-45 0.6180187609220056 1105 46-47 0.44109089357501574 1105 48-49 0.5364964689229907 1105 50-51 0.46294036221424406 1105 52-53 0.5214529093197271 1105 54-55 0.5696759627897947 1105 56-57 0.32406086172909454 1105 58-59 0.3072732062189054 1105 60-61 0.4900713917850119 1105 62-63 0.5105440816972298 1105 64-65 0.3590180991789431 1105 66-67 0.29717189934327237 1105 68-69 0.24557220420488335 1105 70-71 0.24512741989689957 1105 72-73 0.3733581075731003 1105 74-75 0.30338119156684584 1105 76-77 0.17579231338095624 1105 78-79 0.06039460889076764 1105 80-81 -0.032580852750186295 1105 82-83 0.07039269423887262 1105 84-85 0.11578477254819575 1105 86-87 0.06595277653111253 1105 88-89 0.07490507202824759 1105 90-91 -0.10883261285978563 1105 92-93 0.1768350218744139 1105 94-95 0.29341563119727 1105 96-97 1.7751565988428268 1105 98-99 2.0034308126767364 1105 100-101 1.454871359345514 1106 1 0.8122102979286012 1106 2 0.8729067330549185 1106 3 0.9517174402023905 1106 4 0.9257561635070815 1106 5 0.8259872772384682 1106 6 0.9615931868769891 1106 7 0.904594134143764 1106 8 0.9075208368587013 1106 9 1.1504966715385834 1106 10-11 1.090816992123564 1106 12-13 1.0436001396337744 1106 14-15 1.2546445963599098 1106 16-17 1.2656357666538511 1106 18-19 1.3097360369657238 1106 20-21 1.2464548690862927 1106 22-23 0.8868808543133682 1106 24-25 1.1480421320915255 1106 26-27 1.4033903983720322 1106 28-29 1.3422732722813606 1106 30-31 1.2011961863386347 1106 32-33 1.2360074564929562 1106 34-35 1.2960398074426251 1106 36-37 1.4122785626397558 1106 38-39 1.3403488067759923 1106 40-41 1.4251559499463937 1106 42-43 1.4004946345222393 1106 44-45 1.5154802600856527 1106 46-47 1.3765125213357194 1106 48-49 1.1982560127632667 1106 50-51 1.4330264758202098 1106 52-53 1.5663017487327195 1106 54-55 1.3853551485798477 1106 56-57 1.4540096989447875 1106 58-59 1.2816116812369671 1106 60-61 1.5839970900461253 1106 62-63 1.6092020800107996 1106 64-65 1.5128807829625863 1106 66-67 1.5021843896270823 1106 68-69 1.3812095451408766 1106 70-71 1.5808491165979248 1106 72-73 1.459918589660976 1106 74-75 1.4134306514427557 1106 76-77 1.42251455803396 1106 78-79 1.2667713828681322 1106 80-81 1.1151990490853834 1106 82-83 1.274836186519476 1106 84-85 1.357815058224812 1106 86-87 1.2802009356047321 1106 88-89 1.1115942678984183 1106 90-91 1.338369192772447 1106 92-93 1.5610925263445488 1106 94-95 1.968038313387865 1106 96-97 3.4981393047093476 1106 98-99 3.6334078371464322 1106 100-101 2.9003648663205723 1107 1 0.632897837565185 1107 2 0.702720889174806 1107 3 0.703083282428107 1107 4 0.32905544384458096 1107 5 0.7076709014698679 1107 6 0.6881415641896993 1107 7 0.5443171605865373 1107 8 0.4775972148331675 1107 9 0.6773804777536014 1107 10-11 0.707974118436745 1107 12-13 0.6090068454544948 1107 14-15 0.7342742183302988 1107 16-17 0.7732451636263065 1107 18-19 0.6737184452156484 1107 20-21 0.6839935406058544 1107 22-23 0.590522043273026 1107 24-25 0.6532647173618855 1107 26-27 0.8154358529174885 1107 28-29 0.8441783099188811 1107 30-31 0.9102115446533574 1107 32-33 0.909974147975575 1107 34-35 0.9278200760063271 1107 36-37 0.6290491116236794 1107 38-39 0.7313617800023451 1107 40-41 0.7686658901123806 1107 42-43 0.7036249596111617 1107 44-45 0.7652647749338612 1107 46-47 0.6167396561966143 1107 48-49 0.7281584033662938 1107 50-51 0.7577141395365352 1107 52-53 0.7712235784953094 1107 54-55 0.738290737407155 1107 56-57 0.6514345617677435 1107 58-59 0.6860511165362695 1107 60-61 0.7187678182563388 1107 62-63 0.643202135157356 1107 64-65 0.6967281510933923 1107 66-67 0.7179465839083825 1107 68-69 0.550185197938184 1107 70-71 0.7295362839952872 1107 72-73 0.7393050842704021 1107 74-75 0.7803044623452244 1107 76-77 0.7487732780824885 1107 78-79 0.5940588618435072 1107 80-81 0.4721949406672614 1107 82-83 0.6507103972344517 1107 84-85 0.7487210471403571 1107 86-87 0.797636552005315 1107 88-89 0.9127740594444518 1107 90-91 0.9199352169817807 1107 92-93 0.8706768592091336 1107 94-95 1.0968261921757474 1107 96-97 2.748001645129012 1107 98-99 2.799830066721526 1107 100-101 2.4188405466911576 1108 1 0.4935290269562671 1108 2 0.5263312102521454 1108 3 0.48849807871361506 1108 4 0.6205223937811866 1108 5 0.649716613014256 1108 6 0.6289934300019837 1108 7 0.4995054551415805 1108 8 0.582955128687118 1108 9 0.6580616962712256 1108 10-11 0.502465774537896 1108 12-13 0.43829718572107623 1108 14-15 0.5111045647648638 1108 16-17 0.6152223421206386 1108 18-19 0.4564549699046765 1108 20-21 0.4711363977487153 1108 22-23 0.4089653150408381 1108 24-25 0.5049838689227286 1108 26-27 0.6189906622242702 1108 28-29 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1.4711702310069583 2311 92-93 1.6902253916400696 2311 94-95 2.0240849441846898 2311 96-97 3.81823063980843 2311 98-99 4.057368623476073 2311 100-101 3.5669869608713896 2312 1 0.12619373086107544 2312 2 -0.3770170305631133 2312 3 -0.49814053879571674 2312 4 -0.2565863017971637 2312 5 -1.8929398391408725 2312 6 -0.48171686566873007 2312 7 -0.06336506709569534 2312 8 0.20363442087037242 2312 9 0.12117128154439882 2312 10-11 0.0816900421526725 2312 12-13 0.2477654742131179 2312 14-15 0.407476511532586 2312 16-17 -0.41229011190896614 2312 18-19 -0.0733214518242491 2312 20-21 0.2260913527036692 2312 22-23 0.30238819513918 2312 24-25 0.24290201699918867 2312 26-27 0.553567361048998 2312 28-29 0.599397685138257 2312 30-31 0.3395832540250652 2312 32-33 -0.2005675625661354 2312 34-35 0.1826170808033396 2312 36-37 0.48126275383732064 2312 38-39 0.5757422643828249 2312 40-41 0.7382458596923485 2312 42-43 0.6079609839471871 2312 44-45 0.5785431182122025 2312 46-47 0.3178827473397021 2312 48-49 0.7105767957846894 2312 50-51 0.5845176963400966 2312 52-53 0.38700689427862756 2312 54-55 0.4908542099706281 2312 56-57 0.475235895569071 2312 58-59 0.7017665722517279 2312 60-61 0.8147453542338781 2312 62-63 0.9348740668292841 2312 64-65 0.8261794205446655 2312 66-67 0.840549836511638 2312 68-69 0.688983606736592 2312 70-71 0.9135189679779678 2312 72-73 0.9080999830652985 2312 74-75 1.0596129916537542 2312 76-77 0.9761864054956142 2312 78-79 0.9632749610596072 2312 80-81 0.8454931739654974 2312 82-83 1.0965709330949913 2312 84-85 1.1532606516799575 2312 86-87 1.3225868269555932 2312 88-89 1.3084801551505443 2312 90-91 1.3424308294773972 2312 92-93 1.3050491535814288 2312 94-95 1.831937927287484 2312 96-97 3.526009133136501 2312 98-99 3.9648166117080734 2312 100-101 3.5009781388287493 2313 1 -0.011489687021136064 2313 2 -0.40261068220960183 2313 3 -0.7935097243558502 2313 4 -0.1651645710346017 2313 5 -0.3141290040914839 2313 6 0.11464505808005754 2313 7 0.09064821901280595 2313 8 0.2931446702550673 2313 9 0.29336316626571346 2313 10-11 -0.6648402892324867 2313 12-13 0.011950783546218702 2313 14-15 0.17871161195942165 2313 16-17 -0.32537056278695786 2313 18-19 -0.03530401283898499 2313 20-21 -2.098730199164706 2313 22-23 -0.41249129703563625 2313 24-25 0.007190171975274495 2313 26-27 0.11063928667964262 2313 28-29 0.05585024771166758 2313 30-31 0.364817535053227 2313 32-33 0.5208987974824879 2313 34-35 0.3296329244187035 2313 36-37 -0.42050770613349897 2313 38-39 0.374246642981376 2313 40-41 0.44696945941777955 2313 42-43 0.30851396022602984 2313 44-45 0.5222855752164932 2313 46-47 0.4558943623327494 2313 48-49 0.33697139785284946 2313 50-51 0.6332415182525146 2313 52-53 0.5382532791112027 2313 54-55 0.42185155012307973 2313 56-57 0.4467102173180493 2313 58-59 0.38515075610674643 2313 60-61 0.22905843791656366 2313 62-63 0.3744085379619797 2313 64-65 0.261035980329293 2313 66-67 0.38782089217923854 2313 68-69 0.3503663638818395 2313 70-71 0.5631832804725221 2313 72-73 0.49739902015403104 2313 74-75 0.7213599671334023 2313 76-77 0.7783910053722032 2313 78-79 0.6830471453361078 2313 80-81 0.9435067886414181 2313 82-83 1.1919006299679786 2313 84-85 1.01922527479865 2313 86-87 1.0436799801962735 2313 88-89 1.2877817877872566 2313 90-91 1.478302413478719 2313 92-93 1.5810502995276465 2313 94-95 1.9858471894886591 2313 96-97 3.723723064698195 2313 98-99 4.063484163082919 2313 100-101 3.654345029129985 2314 1 -0.18952213490725356 2314 2 -0.15202570735563015 2314 3 -1.0252507549930243 2314 4 -0.113507208613143 2314 5 -0.3558415881391639 2314 6 -0.49200234227109974 2314 7 -0.24403445631702425 2314 8 -1.610372150533891 2314 9 -1.2953735048849566 2314 10-11 -0.6213294478815072 2314 12-13 -0.37066963755882654 2314 14-15 -0.4850021611139752 2314 16-17 -0.4163248204295087 2314 18-19 -1.6125804604956429 2314 20-21 -1.0289685340456245 2314 22-23 -1.6210152075139632 2314 24-25 -1.5902531551119168 2314 26-27 -1.7964390874621756 2314 28-29 -2.6138642351540966 2314 30-31 -1.3016056151018986 2314 32-33 -1.1822738453742119 2314 34-35 -1.4604813916760406 2314 36-37 -2.7892674825900805 2314 38-39 -3.25281143922334 2314 40-41 -4.022321899642456 2314 42-43 -2.8585068592520315 2314 44-45 -2.948655790335078 2314 46-47 -3.0324640205044417 2314 48-49 -1.4102573309518505 2314 50-51 -1.012661434522144 2314 52-53 -2.2816215040192347 2314 54-55 -1.7854684100560831 2314 56-57 -1.8884941663771464 2314 58-59 -3.70672412501127 2314 60-61 -2.142299964957296 2314 62-63 -1.893681594963521 2314 64-65 -1.4101103357083637 2314 66-67 -2.395682693424984 2314 68-69 -2.2987565581022054 2314 70-71 -1.763072497569425 2314 72-73 -1.2320199862519416 2314 74-75 -1.6149347685183493 2314 76-77 -0.5937203510585682 2314 78-79 -0.32616204002482263 2314 80-81 -0.3833066196821484 2314 82-83 -0.22423257165395327 2314 84-85 -0.19466211743903017 2314 86-87 0.06517583329066312 2314 88-89 0.4163822822814254 2314 90-91 0.8672967274545194 2314 92-93 1.0359715540211454 2314 94-95 1.5412719615165855 2314 96-97 3.3493526760498256 2314 98-99 3.624657951323808 2314 100-101 3.2946389822534954 2315 1 -2.3165599211709242 2315 2 -1.7729924804723645 2315 3 -1.4396983201256077 2315 4 -1.541878733210055 2315 5 -1.960090300973917 2315 6 -2.8705314068978147 2315 7 -2.074498012303515 2315 8 -1.3040347939610797 2315 9 -1.0666771358211093 2315 10-11 -3.4645424588546554 2315 12-13 -1.917011856407946 2315 14-15 -1.107014124363367 2315 16-17 -1.9118726919310447 2315 18-19 -1.3042987627527296 2315 20-21 -0.9399325116381547 2315 22-23 -1.2033303712684855 2315 24-25 -3.0037774428652995 2315 26-27 -4.261388494737027 2315 28-29 -5.792923253737214 2315 30-31 -8.398290826551133 2315 32-33 -6.796621887567834 2315 34-35 -6.023101276108726 2315 36-37 -3.6818794985615746 2315 38-39 -6.2963576356877695 2315 40-41 -4.049752696184662 2315 42-43 -3.900930148872323 2315 44-45 -3.081690217984235 2315 46-47 -2.2546254247343924 2315 48-49 -3.054083706124864 2315 50-51 -2.7360043178064437 2315 52-53 -2.0016442753003787 2315 54-55 -3.661661133836091 2315 56-57 -3.5607992326527054 2315 58-59 -2.2880008597023576 2315 60-61 -2.505766150216857 2315 62-63 -3.05002206874509 2315 64-65 -2.4192441132131073 2315 66-67 -2.7610235452570784 2315 68-69 -2.000655963071182 2315 70-71 -2.6265425259630675 2315 72-73 -1.708729810839916 2315 74-75 -2.1215569155606424 2315 76-77 -1.5324762374589405 2315 78-79 -1.408637405535341 2315 80-81 -1.145906263608655 2315 82-83 -0.9756021507709853 2315 84-85 -1.1641567424601682 2315 86-87 -1.6343750609070042 2315 88-89 -1.869562052625792 2315 90-91 -1.7601194651781604 2315 92-93 -0.7416474849220442 2315 94-95 0.017459546883976884 2315 96-97 1.6301333332997672 2315 98-99 1.5373075702272452 2315 100-101 1.2749901052172756 2316 1 -3.276171856693729 2316 2 -2.073885374150759 2316 3 -2.246459290436004 2316 4 -2.78141386359723 2316 5 -2.4465512390359656 2316 6 -6.033041586990731 2316 7 -3.5140730579615926 2316 8 -4.6480210384082845 2316 9 -6.4888715332356846 2316 10-11 -5.402211299194903 2316 12-13 -3.4842464490321277 2316 14-15 -4.917631121089343 2316 16-17 -4.417273256151571 2316 18-19 -2.504790753181929 2316 20-21 -3.1695115739365534 2316 22-23 -3.890697668134546 2316 24-25 -2.8057344729386884 2316 26-27 -3.756101161660638 2316 28-29 -3.530127063444482 2316 30-31 -3.260748815433061 2316 32-33 -3.261174741251182 2316 34-35 -5.392711108028799 2316 36-37 -5.417669802459784 2316 38-39 -5.392721438711966 2316 40-41 -4.801224959576995 2316 42-43 -3.927619608507893 2316 44-45 -3.414792681902796 2316 46-47 -3.635257861668041 2316 48-49 -4.093996281045399 2316 50-51 -5.150495045778559 2316 52-53 -2.942636778408879 2316 54-55 -3.2091053894716843 2316 56-57 -2.4806557356288437 2316 58-59 -2.9959733441650194 2316 60-61 -3.19384913734455 2316 62-63 -2.4048142426929466 2316 64-65 -2.6140961512038174 2316 66-67 -3.231993236684854 2316 68-69 -2.756271430105702 2316 70-71 -2.214804836359992 2316 72-73 -2.258368815475375 2316 74-75 -3.1968968416597825 2316 76-77 -3.5725055519902433 2316 78-79 -3.630328400771365 2316 80-81 -3.802264735240822 2316 82-83 -2.8081108713417855 2316 84-85 -2.488789250892676 2316 86-87 -2.3188344667662726 2316 88-89 -2.1607922598882716 2316 90-91 -1.5431700333355565 2316 92-93 -1.3269151159270791 2316 94-95 -1.5105668231943987 2316 96-97 0.37994937885458313 2316 98-99 0.7598847302719705 2316 100-101 0.43360850946620033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25231.0 3 7294.0 4 7051.0 5 7624.0 6 8718.0 7 10193.0 8 12925.0 9 15507.0 10 18409.0 11 22173.0 12 26963.0 13 30525.0 14 34241.0 15 38279.0 16 42369.0 17 45844.0 18 43711.0 19 43411.0 20 47000.0 21 52509.0 22 59732.0 23 67236.0 24 77147.0 25 88675.0 26 101989.0 27 116816.0 28 134705.0 29 155722.0 30 179828.0 31 208973.0 32 244758.0 33 285357.0 34 331469.0 35 373861.0 36 378460.0 37 288342.0 38 73455.0 39 810.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.592896292919626 19.00853585556235 13.547133002499617 23.85143484901841 2 15.575040337008108 20.158188581419996 40.13566736651657 24.131103715055325 3 19.411481768854937 24.233240258542484 29.626572848285747 26.728705124316825 4 12.876494023904383 16.320156661489634 37.46613545816733 33.33721385643865 5 13.730558472594955 37.21048383960173 34.6532936615975 14.405664026205816 6 32.1502713126997 36.25701915259685 17.589841101780753 14.002868432922696 7 28.243566108196077 32.34642734142339 21.260644368360985 18.149362182019544 8 26.10519525913088 35.119145697070806 20.10172048018074 18.673938563617572 9 26.194561699143502 16.723809760501464 20.31647693106468 36.765151609290356 10-11 24.798937444818968 26.481883739402235 27.898975140460497 20.820203675318297 12-13 25.685161156830016 23.75901549163358 28.11164035520507 22.44418299633134 14-15 22.50305808621737 25.337402318824765 26.233085994545817 25.926453600412046 16-17 22.219249643369317 28.452774507715954 26.910960429423454 22.417015419491275 18-19 22.13042999634629 27.21932454326944 28.943200014587823 21.707045445796442 20-21 22.711880757893642 26.514117277004743 29.110648299776358 21.663353665325253 22-23 22.287733538344863 26.474393131050668 28.983288848552057 22.254584482052415 24-25 22.07165264197508 26.60843773851966 29.17433657827081 22.145573041234446 26-27 21.98104451048683 26.819804984375423 28.980280413863817 22.218870091273928 28-29 21.864751187228418 27.271589390429696 28.907384896662165 21.956274525679724 30-31 22.063024506977296 26.975949894419383 28.784715829847997 22.176309768755328 32-33 21.76091086929017 26.94163405400203 28.823819158404014 22.47363591830379 34-35 22.3731463003842 27.003300931200965 28.716398822972362 21.907153945442477 36-37 22.378113701095504 26.735808981294824 28.789189802679584 22.096887514930092 38-39 21.958456291622152 27.03499064755765 28.72159234486175 22.284960715958448 40-41 22.376163216156883 26.883534620577237 28.734006284528967 22.00629587873691 42-43 22.0621393389029 26.882092373830517 28.66935402689891 22.386414260367673 44-45 22.079118376775934 27.056747684726943 28.672712809391648 22.191421129105464 46-47 22.26779157878291 27.16590047976896 28.265322589146773 22.300985352301357 48-49 22.039671560207967 27.254427829840917 28.278429802361348 22.42747080758977 50-51 22.06624818713928 27.425353131816184 28.292795241614943 22.2156034394296 52-53 22.282469791847255 27.340876592897388 28.06738141557282 22.309272199682535 54-55 21.938837393672095 27.387101794750546 28.25884627486731 22.415214536710046 56-57 21.983367736057435 27.47823301120488 28.142131238619726 22.396268014117958 58-59 22.026148102110714 27.221111286229586 28.13856839654585 22.614172215113854 60-61 21.788878188850834 27.379879309697092 28.30924653563369 22.521995965818377 62-63 21.82044948435777 27.234368581497414 28.415521695889996 22.529660238254817 64-65 21.671924635465288 27.304011121519405 28.288345036249112 22.735719206766195 66-67 21.65351437060832 27.348322555332054 28.119045122769776 22.87911795128985 68-69 21.996081087070017 27.103301269336495 28.156140927373375 22.744476716220117 70-71 21.813837793167487 27.027112482081233 27.9999313584103 23.159118366340977 72-73 21.912020100545984 27.048497379294535 27.9816269460644 23.057855574095083 74-75 21.8099096072617 27.36240167685863 27.826593025841657 23.001095690038007 76-77 21.848977875489968 27.07858575123684 27.898719150012102 23.17371722326109 78-79 21.96729334591559 27.228735672587355 27.743887105801807 23.06008387569525 80-81 22.038607942185163 27.13077427070988 27.660511969359824 23.17010581774514 82-83 21.98173280279677 27.112877282977156 27.767697234530157 23.137692679695917 84-85 21.93748268863992 27.090357290250527 27.732498071333683 23.239661949775865 86-87 21.941284547019144 27.08799343205703 27.635790585452803 23.334931435471027 88-89 22.063904069418125 27.07391801473416 27.337807134535602 23.524370781312115 90-91 22.01162773879802 27.05324777686507 27.445840422525063 23.489284061811848 92-93 22.208504331949932 26.799447973951118 27.48973737937948 23.502310314719466 94-95 21.979363899585973 26.737017336714462 27.554874631174727 23.72874413252484 96-97 22.16513305867549 26.436654008471212 27.530497472395943 23.86771546045736 98-99 22.17606489313769 26.49854974366383 27.231584219901684 24.093801143296798 100-101 22.222840006964283 26.494388598056574 27.369821143200294 23.91295025177885 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4465.0 1 2587.0 2 668.0 3 628.5 4 572.5 5 566.0 6 793.5 7 1335.5 8 1998.0 9 2370.0 10 2219.0 11 1985.5 12 2056.5 13 2304.0 14 2615.0 15 2877.5 16 3195.0 17 3590.5 18 4085.0 19 4773.0 20 5613.5 21 6739.0 22 8457.0 23 10766.5 24 13630.0 25 17352.0 26 22271.0 27 28520.0 28 34256.0 29 39507.0 30 45478.0 31 52698.0 32 62028.0 33 69146.0 34 74267.0 35 80628.0 36 88554.5 37 96430.0 38 102967.0 39 108042.5 40 112701.5 41 117936.5 42 122523.5 43 128088.5 44 133299.5 45 137007.5 46 139398.5 47 142419.5 48 144705.0 49 142180.0 50 138067.0 51 134098.5 52 129857.0 53 125687.5 54 120974.0 55 112992.0 56 102817.5 57 93015.0 58 83745.5 59 73081.0 60 59958.0 61 48475.5 62 39155.0 63 29668.5 64 21532.5 65 16222.5 66 12185.0 67 9010.0 68 6694.5 69 4996.5 70 3703.5 71 2698.5 72 1981.5 73 1476.0 74 1137.5 75 850.0 76 631.5 77 456.0 78 318.5 79 230.0 80 156.5 81 110.0 82 74.0 83 41.5 84 28.5 85 25.0 86 22.5 87 19.0 88 11.0 89 11.0 90 9.0 91 5.5 92 5.0 93 3.5 94 2.5 95 2.5 96 2.0 97 1.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11717384455368202 2 0.11159028428144166 3 0.12286529971041013 4 0.13654097631923073 5 0.1329264976888916 6 0.13330412978459866 7 0.14126137751556922 8 0.14042519216078928 9 0.1481936238439063 10-11 0.1427584190378366 12-13 0.1424617081054953 14-15 0.13036399418230782 16-17 0.13358735385637896 18-19 0.1509044828166607 20-21 0.18180288036183628 22-23 0.19845915315463064 24-25 0.21747562654559424 26-27 0.220442735869007 28-29 0.22268155472212753 30-31 0.22234438320810332 32-33 0.22496083415693094 34-35 0.19270026369509768 36-37 0.18158709059286082 38-39 0.1944535555680234 40-41 0.1833134087446646 42-43 0.1861861100441506 44-45 0.1900703258857091 46-47 0.21101542033689097 48-49 0.22903386604634304 50-51 0.24498882208996708 52-53 0.26658128584807533 54-55 0.25925792056347025 56-57 0.22865623395063595 58-59 0.22196675111239625 60-61 0.2003877742148489 62-63 0.19075815577431843 64-65 0.1817624197801534 66-67 0.17705550544437587 68-69 0.1833134087446646 70-71 0.18696834795668668 72-73 0.22409767508102907 74-75 0.2354670985339243 76-77 0.2529325829603767 78-79 0.2422779631172127 80-81 0.2471062591980389 82-83 0.24393684696621165 84-85 0.22961380105046458 86-87 0.21936378702412962 88-89 0.22173747448285983 90-91 0.22421905682607776 92-93 0.23125919803890257 94-95 0.23588519121131427 96-97 8.639952612566733 98-99 0.29391915220515563 100-101 0.3050862727496364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3707312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.422304222769455 #Duplication Level Percentage of deduplicated Percentage of total 1 74.11995144828987 41.07898498144023 2 13.677446638873272 15.160712172206603 3 5.220493844236467 8.67995394085106 4 2.45105220143989 5.433718430963616 5 1.321078233598791 3.660859988229555 6 0.825740693161891 2.7458671143323308 7 0.5274499905057605 2.046274568527498 8 0.35715977571616814 1.5835694196702061 9 0.253524840788058 1.2645837768766448 >10 1.1524750790671132 10.986706991577428 >50 0.061965584816684835 2.3479255427127033 >100 0.028512977656092207 2.84544721235907 >500 0.0019679324161489343 0.7633623737437278 >1k 0.001082362814376465 0.972771673208486 >5k 9.839661948876924E-5 0.4292618133008511 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9214 0.24853586641750142 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6529 0.17611142520510817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4568 0.12321595808499528 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.3947442243868334E-5 0.0 0.0 0.0 0.0 3 5.3947442243868334E-5 0.0 0.0 0.0 0.0 4 5.3947442243868334E-5 0.0 0.0 0.0 0.0 5 5.3947442243868334E-5 0.0 0.0 0.0 0.0 6 5.3947442243868334E-5 0.0 0.0 0.0 0.0 7 8.09211633658025E-5 0.0 0.0 0.0 0.0 8 8.09211633658025E-5 0.0 0.0 2.6973721121934167E-5 5.3947442243868334E-5 9 8.09211633658025E-5 0.0 0.0 2.6973721121934167E-5 8.09211633658025E-5 10-11 8.09211633658025E-5 0.0 0.0 2.6973721121934167E-5 8.09211633658025E-5 12-13 8.09211633658025E-5 0.0 0.0 4.046058168290125E-5 1.3486860560967082E-4 14-15 8.09211633658025E-5 2.6973721121934167E-5 0.0 8.09211633658025E-5 1.7532918729257208E-4 16-17 8.09211633658025E-5 2.6973721121934167E-5 0.0 1.3486860560967082E-4 2.4276349009740752E-4 18-19 8.09211633658025E-5 2.6973721121934167E-5 0.0 1.61842326731605E-4 2.4276349009740752E-4 20-21 8.09211633658025E-5 2.6973721121934167E-5 0.0 2.1578976897547333E-4 2.697372112193417E-4 22-23 8.09211633658025E-5 2.6973721121934167E-5 0.0 3.911189562680454E-4 2.9671093234127585E-4 24-25 8.09211633658025E-5 2.6973721121934167E-5 0.0 6.60856167487387E-4 3.2368465346321E-4 26-27 8.09211633658025E-5 2.6973721121934167E-5 0.0 0.001429607219462511 3.2368465346321E-4 28-29 1.0789488448773667E-4 2.6973721121934167E-5 0.0 0.004909217244192019 3.2368465346321E-4 30-31 1.0789488448773667E-4 2.6973721121934167E-5 0.0 0.017721734777110747 3.2368465346321E-4 32-33 1.2138174504870376E-4 2.6973721121934167E-5 0.0 0.04848526371667666 3.2368465346321E-4 34-35 1.61842326731605E-4 2.6973721121934167E-5 0.0 0.10080079583266799 3.5065837458514416E-4 36-37 1.61842326731605E-4 2.6973721121934167E-5 0.0 0.18998940472234332 3.5065837458514416E-4 38-39 1.61842326731605E-4 2.6973721121934167E-5 0.0 0.32450735195742897 3.911189562680454E-4 40-41 1.61842326731605E-4 4.046058168290125E-5 0.0 0.4711499868368241 4.046058168290125E-4 42-43 1.7532918729257208E-4 5.3947442243868334E-5 0.0 0.6282853992326516 4.046058168290125E-4 44-45 2.4276349009740752E-4 5.3947442243868334E-5 0.0 0.8169665784805811 4.180926773899796E-4 46-47 2.697372112193417E-4 5.3947442243868334E-5 0.0 1.0435188621837062 4.3157953795094667E-4 48-49 2.697372112193417E-4 5.3947442243868334E-5 0.0 1.281548464224214 4.3157953795094667E-4 50-51 2.697372112193417E-4 5.3947442243868334E-5 0.0 1.5366119711532238 4.3157953795094667E-4 52-53 2.697372112193417E-4 5.3947442243868334E-5 0.0 1.7842037573314573 4.720401196338479E-4 54-55 2.697372112193417E-4 5.3947442243868334E-5 0.0 2.0529159671481656 4.8552698019481503E-4 56-57 2.697372112193417E-4 5.3947442243868334E-5 0.0 2.3662831722822357 4.990138407557821E-4 58-59 2.697372112193417E-4 5.3947442243868334E-5 0.0 2.7030905410712665 5.125007013167492E-4 60-61 2.697372112193417E-4 5.3947442243868334E-5 0.0 3.0562709585813117 5.125007013167492E-4 62-63 2.697372112193417E-4 5.3947442243868334E-5 0.0 3.40238426115741 5.394744224386834E-4 64-65 2.697372112193417E-4 5.3947442243868334E-5 0.0 3.754741980173236 5.394744224386834E-4 66-67 2.9671093234127585E-4 5.3947442243868334E-5 0.0 4.154411606036934 5.394744224386834E-4 68-69 3.2368465346321E-4 5.3947442243868334E-5 0.0 4.596106289408606 5.394744224386834E-4 70-71 3.5065837458514416E-4 5.3947442243868334E-5 0.0 5.059703634331289 5.934218646825517E-4 72-73 3.911189562680454E-4 5.3947442243868334E-5 0.0 5.519619066320828 6.069087252435187E-4 74-75 4.046058168290125E-4 5.3947442243868334E-5 0.0 5.969931853590957 6.203955858044858E-4 76-77 4.046058168290125E-4 5.3947442243868334E-5 0.0 6.454622648430993 6.203955858044858E-4 78-79 4.046058168290125E-4 5.3947442243868334E-5 0.0 6.98523620348112 6.203955858044858E-4 80-81 4.046058168290125E-4 5.3947442243868334E-5 0.0 7.534475112965945 6.203955858044858E-4 82-83 4.046058168290125E-4 5.3947442243868334E-5 0.0 8.09239956065203 6.33882446365453E-4 84-85 4.450663985119137E-4 5.3947442243868334E-5 0.0 8.639089453490831 6.4736930692642E-4 86-87 4.585532590728808E-4 5.3947442243868334E-5 0.0 9.130820389543691 6.60856167487387E-4 88-89 4.585532590728808E-4 5.3947442243868334E-5 0.0 9.625032907939769 6.743430280483542E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6020 0.0 38.216038 1 GTATCAA 10605 0.0 38.107967 1 ATCAACG 13025 0.0 30.450941 3 TCAACGC 13145 0.0 30.244951 4 TATCAAC 13555 0.0 29.467773 2 CAACGCA 13375 0.0 29.409447 5 AACGCAG 13940 0.0 28.454494 6 ACGCAGA 15885 0.0 24.674244 7 GTACATG 11395 0.0 24.648657 1 TACATGG 11155 0.0 24.42014 2 CGCAGAG 16150 0.0 24.1237 8 ACATGGG 11385 0.0 23.266241 3 GCAGAGT 17570 0.0 20.67434 9 AGAGTAC 14605 0.0 20.50554 10-11 CATGGGG 6400 0.0 19.555027 4 GAGTACT 9765 0.0 19.080574 12-13 AGTACTT 10775 0.0 18.842218 12-13 GTACTTT 10455 0.0 17.780272 14-15 CGATACG 80 0.009100945 17.693254 92-93 GAGTACA 8755 0.0 17.625889 1 >>END_MODULE