FastQCFastQC Report
Fri 27 May 2016
SRR939726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3707312
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98480.26563720560880766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77670.2095048919540627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57600.1553686336623408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65000.041.100491
GTATCAA106800.035.9886631
ATCAACG133650.028.3222473
TCAACGC134150.028.2166844
TATCAAC137600.027.7898712
CAACGCA137750.027.4103935
AACGCAG142300.026.5339536
GTACATG109650.023.0659081
ACGCAGA164950.022.8617047
CGCAGAG166700.022.6501588
TACATGG110550.022.01552
ACATGGG112550.020.69333
GCAGAGT183850.020.1244839
AGAGTAC156850.019.61214410-11
TAAGGTG19900.019.3074865
GAGTACT109150.018.94821212-13
GAGTACA87750.018.548081
AGTACTT117000.018.06206312-13
GTAAGGT17100.017.753244
GTACTTT115700.017.65002314-15
Produced by FastQC (version 0.11.5)