FastQCFastQC Report
Fri 27 May 2016
SRR939725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039660
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50430.4850624242540831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36490.3509801281188081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27290.26248966008118035No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA12760.12273243175653578No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA10970.10551526460573649No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.049.4892921
GTATCAA40700.041.492141
TATCAAC52050.033.355742
ATCAACG52800.032.8835263
TCAACGC53950.032.0946464
CGTACGC300.003852240531.8319592-93
CAACGCA54100.031.74265
AACGCAG55900.030.5507536
ACGCAGA65250.026.0275767
CGCAGAG66150.025.600518
TACATGG42550.024.9723112
GCAGAGT69750.023.5991069
GTACATG44050.023.5835111
ACATGGG43750.023.3126473
GTATTAG2955.456968E-1222.5121141
GGACCGA2755.2750693E-1122.4254866
GAGTACT41800.022.41305712-13
TGGTATC11550.022.1779842
GTGGTAT12650.020.9994431
AGAGTAC62500.020.87194610-11
Produced by FastQC (version 0.11.5)