##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939725_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1039660 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.917383567704828 31.0 31.0 33.0 25.0 34.0 2 30.930429178769984 31.0 31.0 34.0 27.0 34.0 3 31.324133851451435 31.0 31.0 34.0 28.0 34.0 4 35.10585191312544 37.0 35.0 37.0 33.0 37.0 5 35.113054267741376 37.0 35.0 37.0 33.0 37.0 6 35.19821672469846 37.0 35.0 37.0 33.0 37.0 7 35.1467537464171 37.0 35.0 37.0 32.0 37.0 8 35.05969259180886 37.0 35.0 37.0 32.0 37.0 9 36.530913952638365 39.0 37.0 39.0 32.0 39.0 10-11 36.39987688282707 39.0 37.0 39.0 32.0 39.0 12-13 36.30365552199758 39.0 37.0 39.0 32.0 39.0 14-15 37.20454571686898 39.0 37.0 41.0 32.0 41.0 16-17 37.05057855452744 39.0 36.0 40.5 32.0 41.0 18-19 36.924665756112574 39.0 36.0 40.0 31.0 41.0 20-21 37.12035280764866 39.0 37.0 41.0 32.0 41.0 22-23 37.060463997845446 39.0 37.0 41.0 31.5 41.0 24-25 36.89180501317738 39.0 36.0 41.0 31.0 41.0 26-27 36.82054469730488 39.0 36.0 41.0 30.5 41.0 28-29 36.88950907027298 39.0 36.0 41.0 31.0 41.0 30-31 36.701768847507836 39.0 36.0 41.0 30.5 41.0 32-33 36.40217330665794 39.0 35.5 40.5 30.0 41.0 34-35 36.23611757690014 39.0 35.0 40.0 30.0 41.0 36-37 36.03787969143759 39.0 35.0 40.0 29.5 41.0 38-39 35.82044225996961 39.0 35.0 40.0 28.5 41.0 40-41 35.60634630552296 38.0 35.0 40.0 27.5 41.0 42-43 35.24346613315892 38.0 35.0 40.0 26.0 41.0 44-45 34.896189619683355 38.0 34.0 40.0 25.0 41.0 46-47 34.50280764865437 38.0 34.0 40.0 24.0 41.0 48-49 34.11125512186676 38.0 33.0 40.0 23.0 41.0 50-51 33.49158955812477 37.5 33.0 39.5 22.0 40.5 52-53 33.6459279956909 38.0 33.0 39.5 22.0 40.0 54-55 33.76251082084528 38.0 33.0 40.0 21.5 41.0 56-57 33.48653021180001 37.5 33.0 40.0 19.5 41.0 58-59 33.11454081141912 37.0 32.5 40.0 18.5 41.0 60-61 32.73678077448396 36.5 32.0 39.0 17.5 40.5 62-63 32.3214214262355 36.0 31.0 39.0 13.0 40.0 64-65 31.815234307369717 35.5 31.0 39.0 9.0 40.0 66-67 31.255635977146376 35.0 30.5 38.0 7.5 40.0 68-69 30.70143652732624 35.0 29.5 38.0 4.5 40.0 70-71 30.162834003424198 34.0 29.0 37.0 2.0 39.0 72-73 29.5423782775138 34.0 28.5 36.5 2.0 39.0 74-75 29.22171238674182 34.0 28.0 36.0 2.0 39.0 76-77 28.700801223476905 34.0 27.5 36.0 2.0 38.0 78-79 28.077115595483136 33.0 26.0 35.0 2.0 37.0 80-81 27.389256583883192 33.0 26.0 35.0 2.0 37.0 82-83 26.687722428486236 32.0 24.5 35.0 2.0 36.0 84-85 25.945345112825347 32.0 23.0 34.0 2.0 36.0 86-87 25.033591751149416 31.0 19.0 34.0 2.0 35.0 88-89 24.058859146259355 31.0 12.0 34.0 2.0 35.0 90-91 22.536267145028184 29.5 4.5 34.0 2.0 35.0 92-93 20.839676432679916 28.0 2.0 33.5 2.0 35.0 94-95 20.418635419271684 26.5 2.0 33.0 2.0 34.5 96-97 20.28598532212454 27.0 2.0 33.0 2.0 34.0 98-99 19.410097531885427 26.5 2.0 33.0 2.0 34.0 100-101 17.54445587980686 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6127865706169935 1101 2 0.7669610907207236 1101 3 0.5004179158833324 1101 4 0.5729556781471103 1101 5 0.5160335773351648 1101 6 0.6270366221044483 1101 7 0.5386123466050989 1101 8 0.585642137459395 1101 9 0.5190497442076207 1101 10-11 0.5986458404317929 1101 12-13 0.6097007650240442 1101 14-15 0.7952853754546325 1101 16-17 0.7846164077690787 1101 18-19 0.6588976158094013 1101 20-21 0.41947223680327994 1101 22-23 0.536669462377894 1101 24-25 0.6394924303511047 1101 26-27 0.8359876439091067 1101 28-29 0.8460600485244143 1101 30-31 0.9453830483982415 1101 32-33 0.9209811847579701 1101 34-35 0.7174046111222694 1101 36-37 0.306052534365449 1101 38-39 0.3736079805041257 1101 40-41 0.4784277289293897 1101 42-43 0.5622300526603041 1101 44-45 0.6830396162972789 1101 46-47 0.622910621941422 1101 48-49 0.6133367796136255 1101 50-51 0.7088985590535444 1101 52-53 0.6477050137682596 1101 54-55 0.6945009606147465 1101 56-57 0.7092888641687267 1101 58-59 0.6414883708557042 1101 60-61 0.5231350182298442 1101 62-63 0.26219322842295867 1101 64-65 0.29798200099975247 1101 66-67 0.49140113455452905 1101 68-69 0.4101393084619005 1101 70-71 0.04726169896283139 1101 72-73 0.04335075794491772 1101 74-75 0.15707487954867005 1101 76-77 0.4864195469695325 1101 78-79 -0.18470473357565353 1101 80-81 -0.09773269472363921 1101 82-83 -0.3770870295670008 1101 84-85 -0.08953894160577391 1101 86-87 -0.6183551589127703 1101 88-89 -4.907601632567676 1101 90-91 -8.837027484608257 1101 92-93 -11.784251620791355 1101 94-95 -9.726329186741204 1101 96-97 -5.2981499752802605 1101 98-99 -3.310653045492625 1101 100-101 -2.2523061346334288 1102 1 1.597145974565258 1102 2 0.8256266227261477 1102 3 0.7862621304406794 1102 4 0.7357441081482676 1102 5 0.7448433354218409 1102 6 0.7348808543908731 1102 7 0.6699653414902613 1102 8 0.7458474402277915 1102 9 0.9125733105897353 1102 10-11 0.8313923411542561 1102 12-13 0.7545207705200525 1102 14-15 0.8900517504779017 1102 16-17 0.949559807675115 1102 18-19 0.8129172419030652 1102 20-21 0.8467803416530657 1102 22-23 0.9020773642296902 1102 24-25 0.94953684170126 1102 26-27 0.9075156248326124 1102 28-29 0.7342870516890585 1102 30-31 0.7701266415512649 1102 32-33 1.0064380188758193 1102 34-35 0.9989309077829986 1102 36-37 0.8922637409181249 1102 38-39 0.6939086657331401 1102 40-41 0.7580960178070484 1102 42-43 0.8709749223072336 1102 44-45 0.9969206009704834 1102 46-47 0.9915443302448352 1102 48-49 0.952045570578882 1102 50-51 0.930845389536934 1102 52-53 0.8145817700554332 1102 54-55 0.7735487316259224 1102 56-57 0.9229739424869408 1102 58-59 0.6783231978641808 1102 60-61 0.7280947288912287 1102 62-63 0.7038929155046674 1102 64-65 0.6209331839727383 1102 66-67 0.6479710890794372 1102 68-69 0.5751988338543796 1102 70-71 0.5115650790122963 1102 72-73 0.3228763277132849 1102 74-75 0.5464906176546727 1102 76-77 0.3528885343730188 1102 78-79 0.3147500971559367 1102 80-81 0.026310599694358672 1102 82-83 0.0816644884856359 1102 84-85 -0.29651001660865006 1102 86-87 0.029861738675190708 1102 88-89 -0.8971095618995548 1102 90-91 -6.782983037799934 1102 92-93 -12.377228244996873 1102 94-95 -9.738255540134993 1102 96-97 -5.760310795782171 1102 98-99 -3.9790695725427714 1102 100-101 -3.059875566150417 1103 1 1.8923564630901097 1103 2 1.0508320584626567 1103 3 0.8254179158833317 1103 4 0.667579334061088 1103 5 0.7017862655072094 1103 6 0.4872516758678884 1103 7 0.4843112713362814 1103 8 0.5267711697174562 1103 9 0.6244260882936388 1103 10-11 0.6128931522597512 1103 12-13 0.5162867865294132 1103 14-15 0.5868176335191393 1103 16-17 0.5787024292744576 1103 18-19 0.5036556803255294 1103 20-21 0.4252518066957478 1103 22-23 0.399438279582192 1103 24-25 0.47336339809304206 1103 26-27 0.6796704396080315 1103 28-29 0.4508987582018378 1103 30-31 0.6017002526993167 1103 32-33 0.6458467761558282 1103 34-35 0.6789637509072222 1103 36-37 0.7427460827525465 1103 38-39 0.6580165826546676 1103 40-41 0.475336331079923 1103 42-43 0.49112790212267043 1103 44-45 0.47067402489942367 1103 46-47 0.48527621333926874 1103 48-49 0.46427763982867276 1103 50-51 0.45177490313956525 1103 52-53 0.47969426108009117 1103 54-55 0.46291493910936765 1103 56-57 0.24961144481388686 1103 58-59 0.31890772569440884 1103 60-61 0.21466727629435667 1103 62-63 0.13988140046597408 1103 64-65 0.07123468917179565 1103 66-67 0.12567532810291837 1103 68-69 0.10637586760168816 1103 70-71 -0.06080281716620206 1103 72-73 0.15504430633201238 1103 74-75 -0.050989636580361264 1103 76-77 0.05201094481899915 1103 78-79 -0.238333765833719 1103 80-81 -0.43966817859460505 1103 82-83 -0.5256085349433484 1103 84-85 -0.46440453300361995 1103 86-87 -0.4465809653643831 1103 88-89 -1.1266876540730522 1103 90-91 -7.643210280307182 1103 92-93 -11.75763871756555 1103 94-95 -10.147431337278837 1103 96-97 -6.391967179581336 1103 98-99 -4.666835841191549 1103 100-101 -3.616284629257084 1104 1 1.9178495197713659 1104 2 1.1200977417176397 1104 3 0.9713694477361408 1104 4 0.7383795718089701 1104 5 0.7306496685438972 1104 6 0.7761099457117311 1104 7 0.8033175932685239 1104 8 0.7314680411715031 1104 9 0.837902070354481 1104 10-11 0.7773052665948725 1104 12-13 0.5334863596639039 1104 14-15 0.8068485677602482 1104 16-17 0.8519855113515149 1104 18-19 0.9555502337377533 1104 20-21 0.6633853507601586 1104 22-23 0.7883661176974996 1104 24-25 0.7054350466584509 1104 26-27 0.6382381437199882 1104 28-29 0.7833945705971459 1104 30-31 0.8263064827744202 1104 32-33 0.9676338026863291 1104 34-35 0.8934785290992551 1104 36-37 0.797674623304772 1104 38-39 0.727320089435878 1104 40-41 0.4885482238772454 1104 42-43 0.5164149556699797 1104 44-45 0.5411500609938784 1104 46-47 0.5599938877327375 1104 48-49 0.46756490951041485 1104 50-51 0.6565697105097499 1104 52-53 0.49579492569348105 1104 54-55 0.48102227687346044 1104 56-57 0.554012482259175 1104 58-59 0.5130315707260209 1104 60-61 0.5322342779694011 1104 62-63 0.4844658820134953 1104 64-65 0.5505161772573857 1104 66-67 0.7289869129086632 1104 68-69 0.7029663468797978 1104 70-71 0.5867089351437365 1104 72-73 0.5322603597172169 1104 74-75 0.44622493088059656 1104 76-77 0.5126123388861039 1104 78-79 0.25026581810749704 1104 80-81 0.19578327220779101 1104 82-83 -0.00821849333746627 1104 84-85 0.40586279827643423 1104 86-87 0.9656049642838624 1104 88-89 0.718758663536498 1104 90-91 -2.5049585377232937 1104 92-93 -8.979329159020136 1104 94-95 -6.306647444697651 1104 96-97 -3.2933517908095205 1104 98-99 -2.4062898315735666 1104 100-101 -1.9937580177046996 1105 1 1.805706157067224 1105 2 1.2552671727407692 1105 3 0.9948208016643569 1105 4 0.7002336021193969 1105 5 0.7866898756902287 1105 6 0.7946405762552189 1105 7 0.6225996128992151 1105 8 0.6365986437720395 1105 9 0.8094708829478492 1105 10-11 0.5738663641695112 1105 12-13 0.5568440586384611 1105 14-15 0.8871070247257933 1105 16-17 0.6377766730874725 1105 18-19 0.7368432708757808 1105 20-21 0.615641283647939 1105 22-23 0.7053920509076974 1105 24-25 0.7640807039089594 1105 26-27 0.593071506992203 1105 28-29 0.4722094823081662 1105 30-31 0.6007795968937515 1105 32-33 0.720436158492241 1105 34-35 0.7048966032770494 1105 36-37 0.6032672825988001 1105 38-39 0.6205561290903105 1105 40-41 0.3088949928581073 1105 42-43 0.44319059985485154 1105 44-45 0.5671274118483609 1105 46-47 0.5533374770511656 1105 48-49 0.3379142117810119 1105 50-51 0.39612641551353533 1105 52-53 0.44529034043906535 1105 54-55 0.43010507245846696 1105 56-57 0.455759991574773 1105 58-59 0.41057266759441546 1105 60-61 0.5542754339478861 1105 62-63 0.5997635742633136 1105 64-65 0.7141839662481715 1105 66-67 0.7046266453318566 1105 68-69 0.5111406587078058 1105 70-71 0.39776582361910684 1105 72-73 0.41878318158703465 1105 74-75 0.30477601198118265 1105 76-77 0.2842719660779771 1105 78-79 0.40673253912474294 1105 80-81 0.18402420187906543 1105 82-83 0.14025032493660916 1105 84-85 0.5640352677119083 1105 86-87 0.7179240758821805 1105 88-89 -0.10342474049864592 1105 90-91 -3.354029741588633 1105 92-93 -9.377075605889374 1105 94-95 -6.649732791996321 1105 96-97 -3.6993582975258086 1105 98-99 -2.6222178523680384 1105 100-101 -1.959354024011823 1106 1 1.6755450486980514 1106 2 0.8217067482889604 1106 3 0.5288298265533058 1106 4 0.638547075996577 1106 5 0.5670157940431864 1106 6 0.5077231398877373 1106 7 0.505061891683674 1106 8 -0.16863825956293965 1106 9 -0.47665951964680175 1106 10-11 -0.6195355077898768 1106 12-13 -0.4173825683092929 1106 14-15 0.5115539858764677 1106 16-17 0.4657216600436911 1106 18-19 0.661983844097243 1106 20-21 0.5371934940406717 1106 22-23 0.55526954509088 1106 24-25 0.6022870953635078 1106 26-27 0.44415555127056194 1106 28-29 0.3994564505095326 1106 30-31 0.6190699797464632 1106 32-33 0.6622084386868394 1106 34-35 0.8186194581032495 1106 36-37 0.9496009214622205 1106 38-39 0.8071946223568958 1106 40-41 0.6662017156953084 1106 42-43 0.9294963884750302 1106 44-45 0.6039866799862779 1106 46-47 0.7024288187983245 1106 48-49 0.6291814859825209 1106 50-51 0.7286979800626412 1106 52-53 0.6751967424696588 1106 54-55 0.7061221351391396 1106 56-57 0.6520773257071895 1106 58-59 0.7770342765628939 1106 60-61 0.5536095839866633 1106 62-63 0.6811810282575337 1106 64-65 0.8641782375588889 1106 66-67 0.6741244596165643 1106 68-69 0.6004980760384129 1106 70-71 0.41793477588590733 1106 72-73 0.7445759440491351 1106 74-75 0.3882628448092156 1106 76-77 0.36669766938475234 1106 78-79 0.38506883962534033 1106 80-81 0.057338645226732154 1106 82-83 0.1123877429722846 1106 84-85 0.31090564069364746 1106 86-87 0.5928421115586957 1106 88-89 0.12556110523215835 1106 90-91 -4.033145143830755 1106 92-93 -10.1909513726524 1106 94-95 -6.882418930330946 1106 96-97 -3.314284177099946 1106 98-99 -2.412772565757308 1106 100-101 -1.6549477806218498 1107 1 1.8157777922624838 1107 2 0.9644899892336021 1107 3 0.6852919725021245 1107 4 0.6463456939630134 1107 5 0.46295796884765394 1107 6 0.48198466316209476 1107 7 0.4426321324005116 1107 8 0.44435627305153247 1107 9 0.5942124144408254 1107 10-11 0.41675663664022267 1107 12-13 0.5621522293215051 1107 14-15 0.4965400326529874 1107 16-17 0.45170341644129053 1107 18-19 0.530600956254581 1107 20-21 0.42992452904712053 1107 22-23 0.6173708815628984 1107 24-25 0.4705786323063279 1107 26-27 0.41159613142090734 1107 28-29 0.24778918268357586 1107 30-31 0.5492478596158534 1107 32-33 0.643293278082389 1107 34-35 0.639834527584604 1107 36-37 0.2911112867139565 1107 38-39 0.483467972649251 1107 40-41 0.5306053340414252 1107 42-43 0.41082944337669147 1107 44-45 -0.001702818077362167 1107 46-47 -0.05722927976648862 1107 48-49 -0.09972778958889705 1107 50-51 -0.025902786890050322 1107 52-53 0.10757296693621754 1107 54-55 -0.09657738646462377 1107 56-57 -0.05260649614717039 1107 58-59 0.20925578161263303 1107 60-61 0.03035088932495711 1107 62-63 -0.012539546577301053 1107 64-65 -0.06258015335917122 1107 66-67 0.1843444839160533 1107 68-69 -0.1373858749069896 1107 70-71 0.06522813529057103 1107 72-73 -0.12956265797075872 1107 74-75 -0.3949519968644104 1107 76-77 -0.17233386300191356 1107 78-79 -0.2943757841122263 1107 80-81 -0.4681781935613287 1107 82-83 -0.5141195848430371 1107 84-85 -0.6401413256667396 1107 86-87 -0.23194621709192376 1107 88-89 0.16483942932661932 1107 90-91 -3.944371793538398 1107 92-93 -5.559422782782249 1107 94-95 -3.3434400784819047 1107 96-97 -1.3954326128201977 1107 98-99 -0.6658622080991705 1107 100-101 -0.4186394998108547 1108 1 1.488572810410023 1108 2 0.8439378067042291 1108 3 -0.3707074782471551 1108 4 -0.13852059317635934 1108 5 0.3247731682479298 1108 6 0.5462135967992481 1108 7 0.4890772184621639 1108 8 0.5601934350582312 1108 9 0.5148456856741888 1108 10-11 0.6842853424125579 1108 12-13 0.5376456271794368 1108 14-15 0.5618540147949105 1108 16-17 0.5188340082218303 1108 18-19 0.5295409580359376 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60-61 -0.21459545185165751 2311 62-63 -0.09633920007320995 2311 64-65 0.2901328485098986 2311 66-67 0.3203032448083185 2311 68-69 0.2818755422321608 2311 70-71 0.5615748593854981 2311 72-73 0.3193260918360288 2311 74-75 0.17832925654349552 2311 76-77 0.4152101019569834 2311 78-79 0.7113319557119446 2311 80-81 0.6391456170733285 2311 82-83 0.8004779668694262 2311 84-85 0.8290474052691295 2311 86-87 1.2227033766144828 2311 88-89 1.7674905554649243 2311 90-91 2.430265131052863 2311 92-93 3.383843573035918 2311 94-95 2.932556500098375 2311 96-97 2.4218741950274456 2311 98-99 2.3098545309692113 2311 100-101 2.2127584744583224 2312 1 1.1556857983593467 2312 2 0.5866378788022821 2312 3 0.07416326024290498 2312 4 -0.7131163290914913 2312 5 -0.20885542378008637 2312 6 -0.07516862588074247 2312 7 0.024147771557224473 2312 8 0.2869804368091806 2312 9 0.2361867094665513 2312 10-11 0.10666406318708965 2312 12-13 0.225486805467618 2312 14-15 0.3108304799303596 2312 16-17 0.09083550168151078 2312 18-19 -0.08122611375312516 2312 20-21 0.25153565872529526 2312 22-23 0.3821832136141339 2312 24-25 0.5837478435194612 2312 26-27 0.431935921585179 2312 28-29 0.4597050204327573 2312 30-31 0.2844451867312543 2312 32-33 0.5680068354955168 2312 34-35 0.5028274590064612 2312 36-37 0.44500209563052806 2312 38-39 0.4099766861834766 2312 40-41 0.558335352606349 2312 42-43 0.6180745910943273 2312 44-45 0.2247867703069133 2312 46-47 0.2872079095705615 2312 48-49 0.6832355654646847 2312 50-51 0.5932377211434741 2312 52-53 0.669813256093029 2312 54-55 0.6059038602633322 2312 56-57 0.6039072594720523 2312 58-59 0.7214833206694777 2312 60-61 0.7078424230653937 2312 62-63 0.7292965257737194 2312 64-65 0.5593470242516503 2312 66-67 0.7469247757387976 2312 68-69 0.8317593661181988 2312 70-71 0.8391403682387626 2312 72-73 0.7424543498898721 2312 74-75 1.0621724112701543 2312 76-77 1.1474941845134587 2312 78-79 1.0915088893016893 2312 80-81 1.2105685488847193 2312 82-83 1.2014928159814673 2312 84-85 1.6211400980171504 2312 86-87 1.7025873320915856 2312 88-89 2.25865891495668 2312 90-91 3.204739659721856 2312 92-93 4.418140904933029 2312 94-95 3.9473172581381206 2312 96-97 3.4140567456759072 2312 98-99 2.846438257665845 2312 100-101 2.900257886998201 2313 1 -6.8992718011188 2313 2 -2.295890931808721 2313 3 -1.183990686267208 2313 4 -0.47117514622564727 2313 5 -0.33014537811798306 2313 6 0.15163339629799566 2313 7 0.20613026775205157 2313 8 0.2936938527691737 2313 9 0.3659839479966607 2313 10-11 0.31265313689876706 2313 12-13 0.16376822534150648 2313 14-15 0.020291775864258454 2313 16-17 0.046559572131599225 2313 18-19 -0.044968231604833875 2313 20-21 0.14113122297839453 2313 22-23 -0.44068588836455547 2313 24-25 0.3047062040326196 2313 26-27 0.15085579547080386 2313 28-29 0.09375590105897658 2313 30-31 0.5171316403081221 2313 32-33 0.3153296230580267 2313 34-35 0.30910327983707475 2313 36-37 0.4220215563828589 2313 38-39 0.36828283969511233 2313 40-41 0.32533633107992443 2313 42-43 0.36927050324914745 2313 44-45 0.39849980574940247 2313 46-47 -0.5684808271113226 2313 48-49 -0.09812315382212233 2313 50-51 -0.19227271590805373 2313 52-53 -0.37181943579651744 2313 54-55 -0.07047279770578996 2313 56-57 0.1658364832163457 2313 58-59 0.12653061355923256 2313 60-61 -0.22174145386437516 2313 62-63 0.04006381214031762 2313 64-65 0.16536871374936624 2313 66-67 0.3179660347081388 2313 68-69 0.2522380027783413 2313 70-71 0.3584720164231463 2313 72-73 0.22813058385377616 2313 74-75 0.19114746019383233 2313 76-77 0.6077233104103925 2313 78-79 0.15989332069981899 2313 80-81 0.2893288772169633 2313 82-83 0.5646084389429831 2313 84-85 0.9316016113896204 2313 86-87 1.266018240984824 2313 88-89 1.726301337222388 2313 90-91 2.5828041846185776 2313 92-93 3.9647339142828883 2313 94-95 3.732202559290542 2313 96-97 2.4090216837059124 2313 98-99 2.256717666232923 2313 100-101 1.6049423292682583 2314 1 0.7520674845954858 2314 2 0.06561700469921661 2314 3 -1.058990686267208 2314 4 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-1.5894675957744404 2314 66-67 -0.8620934891013832 2314 68-69 -0.9001429496026105 2314 70-71 -0.6589883010371693 2314 72-73 -1.6191660431303525 2314 74-75 -1.6520817064728348 2314 76-77 -0.9792558562562732 2314 78-79 -1.676589814220815 2314 80-81 -0.6748328291322387 2314 82-83 -0.3830850134379702 2314 84-85 -1.581121602896097 2314 86-87 -0.4767892986977138 2314 88-89 0.7704729642065189 2314 90-91 2.1785135100153994 2314 92-93 3.356003755552731 2314 94-95 2.9320041465921314 2314 96-97 1.9781437075154393 2314 98-99 1.9972434598837197 2314 100-101 2.1829929245063546 2315 1 -2.181426931212073 2315 2 -1.702166500455423 2315 3 -2.3314706404481917 2315 4 -3.6880279526403115 2315 5 -3.6006718876908863 2315 6 -2.562498906107507 2315 7 -5.3409058147221735 2315 8 -2.766750428035934 2315 9 -2.258617181114772 2315 10-11 -3.509608972412309 2315 12-13 -2.5712843437961155 2315 14-15 -1.8535525213047848 2315 16-17 -2.231893217919122 2315 18-19 -1.5923827300502609 2315 20-21 -1.3282682206478498 2315 22-23 -0.6009501658968688 2315 24-25 -2.1812924050330054 2315 26-27 -3.3737821524918843 2315 28-29 -2.8340382406526885 2315 30-31 -2.7404980438679516 2315 32-33 -3.451719550563311 2315 34-35 -3.029763108642719 2315 36-37 -2.3087610487554144 2315 38-39 -1.5411427862253646 2315 40-41 -1.121757025162914 2315 42-43 -2.730590403312796 2315 44-45 -6.0299744619645566 2315 46-47 -2.8367982056091137 2315 48-49 -1.7729359913282536 2315 50-51 -2.7482680521868943 2315 52-53 -2.5990377715844346 2315 54-55 -1.3333747368319564 2315 56-57 -0.8557741369767484 2315 58-59 -2.3403835576075096 2315 60-61 -2.4055646824685297 2315 62-63 -4.082210570121177 2315 64-65 -3.5085952446862407 2315 66-67 -2.7917899381277316 2315 68-69 -1.544895240553359 2315 70-71 -3.1294851509798995 2315 72-73 -2.0756863037259983 2315 74-75 -1.0896013943307956 2315 76-77 -3.0694316151337055 2315 78-79 -1.4418085284247084 2315 80-81 -1.0462084734621406 2315 82-83 -0.5937075077105938 2315 84-85 -2.270298650433322 2315 86-87 -1.8263919619279534 2315 88-89 -0.8864768496537323 2315 90-91 0.19023852433384292 2315 92-93 0.92774630996281 2315 94-95 0.9972676059277532 2315 96-97 0.5993188936551839 2315 98-99 0.43424589401888625 2315 100-101 -0.14936854456581017 2316 1 -7.2039214525426445 2316 2 -5.6585972219343645 2316 3 -2.890152803719648 2316 4 -3.0875075145741455 2316 5 -1.4593179528550735 2316 6 -1.8983562645787657 2316 7 -2.2708996467778633 2316 8 -1.7376829245753456 2316 9 -6.10212478019271 2316 10-11 -5.999451760891613 2316 12-13 -4.9308285980859665 2316 14-15 -3.628411895016832 2316 16-17 -4.099089134000977 2316 18-19 -3.335891466076454 2316 20-21 -2.0059206498799327 2316 22-23 -2.8487316956039095 2316 24-25 -2.4086465274652724 2316 26-27 -3.39126628495773 2316 28-29 -3.465974690929677 2316 30-31 -5.817437464422277 2316 32-33 -3.9762065737201056 2316 34-35 -4.465639226760274 2316 36-37 -3.070374264641991 2316 38-39 -5.567417660521514 2316 40-41 -2.8314564728903022 2316 42-43 -3.953940336090728 2316 44-45 -2.6423532703859323 2316 46-47 -3.502440908248822 2316 48-49 -4.414661566126661 2316 50-51 -3.7889509033120454 2316 52-53 -5.255355366711477 2316 54-55 -3.9762599988558947 2316 56-57 -3.034011383970231 2316 58-59 -2.1064024480028607 2316 60-61 -2.124259522713082 2316 62-63 -2.951247631792093 2316 64-65 -2.1823323083468154 2316 66-67 -3.2145542004330636 2316 68-69 -2.5328300877332985 2316 70-71 -1.884003809722035 2316 72-73 -1.2314631378367231 2316 74-75 -0.9931732594091471 2316 76-77 -1.6287582859502372 2316 78-79 -3.836069497843642 2316 80-81 -4.9825962182968375 2316 82-83 -2.547847989037642 2316 84-85 -1.1819293468994019 2316 86-87 -1.5832434963817512 2316 88-89 -0.6990821950159827 2316 90-91 -0.41647330760246604 2316 92-93 -0.07196874237944684 2316 94-95 -1.198951188395462 2316 96-97 -3.4424014227575164 2316 98-99 -5.025210531017853 2316 100-101 -4.002388847619368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5760.0 3 1503.0 4 1305.0 5 1458.0 6 1623.0 7 1844.0 8 2040.0 9 2537.0 10 2974.0 11 3832.0 12 4863.0 13 6035.0 14 6931.0 15 8519.0 16 10391.0 17 12133.0 18 10087.0 19 8560.0 20 8849.0 21 9464.0 22 10619.0 23 12302.0 24 14036.0 25 16371.0 26 19394.0 27 23602.0 28 28274.0 29 34601.0 30 42876.0 31 52436.0 32 64008.0 33 78019.0 34 98510.0 35 125536.0 36 145588.0 37 123013.0 38 38937.0 39 830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.496654888716186 19.666384496634663 13.263050845498906 23.573909769150244 2 15.485532713879929 20.026190361561795 40.35790284531318 24.1303740792451 3 19.34825648353934 24.30166898416526 29.506122433257964 26.843952099037438 4 12.498410986344114 15.942814769159652 36.91163157996109 34.64714266453514 5 13.386487943918034 37.38637239041676 34.37938140358987 14.847758262075342 6 32.15793528505393 35.87365177195686 17.409476117103235 14.55893682588598 7 28.1757514342849 32.78289800683525 20.759564146923175 18.281786411956677 8 26.446820683424484 34.29788045561632 19.900947948551494 19.354350912407707 9 26.349088310004458 16.271515912792943 20.08174337051247 37.297652406690126 10-11 24.659523962029077 26.450240821871763 27.478158436278406 21.41207677982076 12-13 25.628421405429563 23.875390536214407 27.91914985418697 22.57703820416906 14-15 22.480926268463943 25.148720447935922 26.413271647595742 25.957081636004386 16-17 21.690291277096552 28.41983812374557 27.123133049029015 22.766737550128862 18-19 21.58435258693596 26.61041748139529 29.829807605246884 21.975422326421867 20-21 22.181181547590377 25.99419147890919 29.983479910993804 21.84114706250662 22-23 21.735519113045974 26.038471804270387 29.980275563957953 22.24573351872569 24-25 21.41125458934102 26.230671037674156 30.26124367467252 22.096830698312296 26-27 21.101916691165485 26.5350156390818 29.99026473182228 22.372802937930437 28-29 21.145234542769792 27.097333225382357 29.909215157859027 21.848217073988828 30-31 21.281252590126773 26.549594542759497 29.77233869895161 22.396814168162113 32-33 21.022085496971872 26.594924460584192 29.850983612245134 22.532006430198802 34-35 21.713917867280333 26.862667720922794 29.622958884492945 21.80045552730393 36-37 21.699779781323336 26.264040747683364 29.760024286698695 22.276155184294602 38-39 21.15422975703723 26.78309696828947 29.715070668602944 22.34760260607036 40-41 21.55882316093599 26.680486208887107 29.750612194155895 22.010078436021015 42-43 21.25435070015919 26.66738295026615 29.608514074490927 22.46975227508374 44-45 21.27762901409021 26.967093301008948 29.675529213837176 22.07974847106367 46-47 21.68213166959873 27.140200767973884 28.931914957180325 22.245752605247066 48-49 21.278676592862876 27.322602180132023 28.96548566140231 22.43323556560279 50-51 21.39361132935829 27.52367914933409 28.911024240700662 22.171685280606958 52-53 21.749055844365554 27.307174136763386 28.71245369600478 22.23131632286628 54-55 21.280102108937086 27.488146176192867 28.854643705652094 22.377108009217952 56-57 21.292117694624096 27.85756519836717 28.597650213211757 22.25266689379698 58-59 21.301642268845562 27.33412140134749 28.73659653889395 22.627639790912994 60-61 20.956152112130496 27.598061911119053 28.972280048736472 22.473505928013978 62-63 21.07599382206348 27.426540654233072 29.204576220725375 22.292889302978068 64-65 20.910698198740707 27.570973662785374 28.943717271181296 22.57461086729262 66-67 20.897101438802128 27.73980637717187 28.592922336481692 22.770169847544306 68-69 21.38722141391172 27.31574535328522 28.88151238642167 22.415520846381394 70-71 21.221741170756996 27.29021664660769 28.566410958851307 22.921631223784004 72-73 21.213264724351998 27.451833709487524 28.57022372137698 22.764677844783503 74-75 20.968003123057123 27.93907146884895 28.392589486671582 22.70033592142234 76-77 21.1432782743195 27.57630924820804 28.38144429775537 22.898968179717095 78-79 21.328652959398255 27.765147151746973 28.271036258117192 22.635163630737583 80-81 21.353575913290157 27.790822388955437 28.013815343901317 22.841786353853088 82-83 21.22732029653596 27.71330936274848 28.262842813669113 22.796527527046447 84-85 21.20134650277951 27.76311382382211 28.15352252163248 22.8820171517659 86-87 21.141842030181287 27.633392580369055 28.198906178913198 23.025859210536463 88-89 21.308723538381756 27.742166821212866 27.84164187822788 23.107467762177503 90-91 20.969641938607698 27.689710715760548 28.13135277028479 23.209294575346963 92-93 21.428422871752556 27.327931991655667 28.151530995482005 23.092114141109768 94-95 20.973889749859598 27.193336279718768 28.418997238246142 23.413776732175496 96-97 21.488810156167514 26.976295494220317 28.06146037574117 23.473433973871 98-99 21.34000876705247 27.205880580014302 27.67644657571372 23.777664077219505 100-101 21.40160906012753 27.11083028660178 27.919894272787783 23.567666380482912 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1606.0 1 1010.0 2 460.0 3 532.0 4 465.5 5 419.5 6 625.5 7 1184.0 8 1826.5 9 2018.5 10 1774.0 11 1491.5 12 1379.0 13 1434.0 14 1495.0 15 1499.5 16 1550.5 17 1688.5 18 1846.5 19 2065.5 20 2321.5 21 2612.5 22 3129.5 23 3851.5 24 4600.5 25 5616.0 26 7331.0 27 9365.0 28 10754.5 29 11908.5 30 13475.0 31 15715.5 32 18762.0 33 20503.0 34 21536.5 35 23243.5 36 25484.5 37 27658.0 38 28606.0 39 29181.5 40 30009.5 41 31240.0 42 32188.5 43 33019.0 44 34232.0 45 35573.5 46 36128.0 47 36546.5 48 37286.5 49 36670.0 50 35568.5 51 34671.5 52 33910.5 53 33782.5 54 33377.0 55 31399.5 56 29165.5 57 26850.5 58 24916.0 59 22547.0 60 18523.0 61 15147.5 62 12198.0 63 8930.0 64 6391.5 65 4840.0 66 3524.0 67 2576.0 68 1985.0 69 1476.5 70 1111.0 71 770.5 72 504.0 73 378.5 74 281.0 75 205.5 76 156.0 77 95.0 78 68.0 79 55.5 80 33.0 81 20.0 82 16.0 83 15.0 84 11.0 85 6.0 86 9.0 87 7.5 88 5.0 89 4.0 90 2.0 91 2.0 92 2.0 93 1.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12302098763057154 2 0.10676567339322472 3 0.11388338495277302 4 0.12311717292191678 5 0.11349864378739202 6 0.12119346709501184 7 0.1454321605140142 8 0.13639074312756094 9 0.14745205163226438 10-11 0.13893965334820998 12-13 0.12927303156801262 14-15 0.13336090645018564 16-17 0.12850354923725063 18-19 0.14254660177365677 20-21 0.14716349575822865 22-23 0.17900082719350557 24-25 0.1986707192736087 26-27 0.21112671450281822 28-29 0.20699074697497258 30-31 0.1987188119192813 32-33 0.19775695900582885 34-35 0.18866744897370294 36-37 0.19766077371448357 38-39 0.18554142700498238 40-41 0.19227439739914973 42-43 0.18236731239058923 44-45 0.1776542331146721 46-47 0.19184156358809612 48-49 0.224833118519516 50-51 0.2265163611180578 52-53 0.22675682434642094 54-55 0.22473693322817073 56-57 0.1997287574784064 58-59 0.20213338976203757 60-61 0.17635573168151125 62-63 0.17053652155512378 64-65 0.17072889213781428 66-67 0.1748167670199873 68-69 0.1760190831618029 70-71 0.181501644768482 72-73 0.2103091395263836 74-75 0.2130504203297232 76-77 0.2230055979839563 78-79 0.2191581863301464 80-81 0.23156608891368333 82-83 0.23243175653579057 84-85 0.20751976607737144 86-87 0.21122289979416345 88-89 0.2129542350383779 90-91 0.4263413038878095 92-93 0.5679260527480138 94-95 0.32337494950272205 96-97 0.24397399149722024 98-99 0.27157917011330623 100-101 0.29144143277609985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1039660.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.69927783317388 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93302551340011 51.99840808210372 2 10.053510285189702 12.606956691395608 3 2.972099841985907 5.590455412218198 4 1.2658492863588635 3.174713444013571 5 0.6836058056362999 2.143079516798051 6 0.41817587811789053 1.5731595339147044 7 0.2937199616530551 1.2891220636583856 8 0.20792962640101992 1.0429629932372475 9 0.16413785840669565 0.9262192668465209 >10 0.9078772998310419 10.55063023670811 >50 0.06427230403881622 2.7659579126236147 >100 0.03267002193106553 3.8760569844177812 >500 0.0021884219346863724 1.0101778419893965 >1k 7.815792623879902E-4 0.9578410837064493 >5k 1.5631585247759803E-4 0.49425893636873264 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5043 0.4850624242540831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3649 0.3509801281188081 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2729 0.26248966008118035 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1276 0.12273243175653578 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1097 0.10551526460573649 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.618529134524749E-5 2 0.0 0.0 0.0 0.0 9.618529134524749E-5 3 0.0 0.0 0.0 0.0 9.618529134524749E-5 4 0.0 0.0 0.0 0.0 9.618529134524749E-5 5 0.0 0.0 0.0 0.0 9.618529134524749E-5 6 0.0 0.0 0.0 0.0 1.9237058269049498E-4 7 0.0 0.0 0.0 0.0 1.9237058269049498E-4 8 0.0 0.0 0.0 0.0 1.9237058269049498E-4 9 0.0 0.0 0.0 0.0 1.9237058269049498E-4 10-11 0.0 0.0 0.0 0.0 1.9237058269049498E-4 12-13 0.0 0.0 0.0 0.0 5.771117480714849E-4 14-15 0.0 0.0 0.0 0.0 5.771117480714849E-4 16-17 0.0 0.0 0.0 0.0 5.771117480714849E-4 18-19 0.0 0.0 0.0 9.618529134524749E-5 5.771117480714849E-4 20-21 0.0 0.0 0.0 1.9237058269049498E-4 6.732970394167324E-4 22-23 0.0 0.0 0.0 5.290191023988611E-4 7.694823307619799E-4 24-25 0.0 0.0 0.0 8.175749764346037E-4 7.694823307619799E-4 26-27 0.0 0.0 0.0 0.0013946867245060886 7.694823307619799E-4 28-29 0.0 0.0 0.0 0.003174114614393167 7.694823307619799E-4 30-31 0.0 0.0 0.0 0.012407902583536926 7.694823307619799E-4 32-33 0.0 0.0 0.0 0.03275109170305677 7.694823307619799E-4 34-35 0.0 0.0 0.0 0.06324182905950022 7.694823307619799E-4 36-37 0.0 0.0 0.0 0.12547371255987533 7.694823307619799E-4 38-39 0.0 0.0 0.0 0.22257276417290267 8.656676221072274E-4 40-41 0.0 0.0 0.0 0.32116268780178137 8.656676221072274E-4 42-43 0.0 0.0 0.0 0.42624511859646425 8.656676221072274E-4 44-45 0.0 0.0 0.0 0.5536906296289171 8.656676221072274E-4 46-47 0.0 0.0 0.0 0.717830829309582 8.656676221072274E-4 48-49 0.0 0.0 0.0 0.8865879229748187 9.13760267779851E-4 50-51 0.0 0.0 0.0 1.0716003308774023 0.0010580382047977223 52-53 0.0 0.0 0.0 1.2535348094569378 0.0011542234961429698 54-55 0.0 0.0 0.0 1.4452801877536887 0.0012504087874882174 56-57 0.0 0.0 0.0 1.673383606178943 0.001298501433160841 58-59 0.0 9.618529134524749E-5 0.0 1.9335648192678376 0.0013465940788334647 60-61 0.0 9.618529134524749E-5 0.0 2.206394398168632 0.0013465940788334647 62-63 0.0 9.618529134524749E-5 0.0 2.4749437316045633 0.0015389646615239598 64-65 0.0 9.618529134524749E-5 0.0 2.749408460458227 0.0015389646615239598 66-67 0.0 9.618529134524749E-5 0.0 3.0639824558028588 0.0015389646615239598 68-69 0.0 9.618529134524749E-5 0.0 3.4185214397014407 0.0015389646615239598 70-71 0.0 9.618529134524749E-5 0.0 3.7979724140584423 0.0015389646615239598 72-73 0.0 9.618529134524749E-5 0.0 4.173095050304907 0.0015389646615239598 74-75 0.0 9.618529134524749E-5 0.0 4.548506242425408 0.0015389646615239598 76-77 0.0 9.618529134524749E-5 0.0 4.9461362368466615 0.0015389646615239598 78-79 0.0 9.618529134524749E-5 0.0 5.379979993459401 0.0015389646615239598 80-81 0.0 9.618529134524749E-5 0.0 5.835224977396457 0.0015389646615239598 82-83 0.0 9.618529134524749E-5 0.0 6.274791758844238 0.0015389646615239598 84-85 0.0 9.618529134524749E-5 0.0 6.69074505126676 0.0015389646615239598 86-87 9.618529134524749E-5 9.618529134524749E-5 0.0 7.127811015139565 0.0015870573071965835 88-89 9.618529134524749E-5 9.618529134524749E-5 0.0 7.581468941769424 0.0016351499528692072 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2195 0.0 49.489292 1 GTATCAA 4070 0.0 41.49214 1 TATCAAC 5205 0.0 33.35574 2 ATCAACG 5280 0.0 32.883526 3 TCAACGC 5395 0.0 32.094646 4 CGTACGC 30 0.0038522405 31.83195 92-93 CAACGCA 5410 0.0 31.7426 5 AACGCAG 5590 0.0 30.550753 6 ACGCAGA 6525 0.0 26.027576 7 CGCAGAG 6615 0.0 25.60051 8 TACATGG 4255 0.0 24.972311 2 GCAGAGT 6975 0.0 23.599106 9 GTACATG 4405 0.0 23.583511 1 ACATGGG 4375 0.0 23.312647 3 GTATTAG 295 5.456968E-12 22.512114 1 GGACCGA 275 5.2750693E-11 22.425486 6 GAGTACT 4180 0.0 22.413057 12-13 TGGTATC 1155 0.0 22.177984 2 GTGGTAT 1265 0.0 20.999443 1 AGAGTAC 6250 0.0 20.871946 10-11 >>END_MODULE