FastQCFastQC Report
Fri 27 May 2016
SRR939725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1039660
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.5592212838812689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50300.4838120154665949No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39300.3780081949868226No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.12465613758344074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.11744224073254718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.048.229311
GTATCAA47900.036.1042251
GTAAGGT5400.030.5334844
GGTAAGG5850.027.3794773
TATCAAC63950.027.0350742
AGGTAAG5450.026.7958342
ATCAACG64500.026.7315083
TAAGGTG6600.026.4094835
TCAACGC65850.026.111944
CAACGCA67250.025.5683485
AACGCAG69650.024.8130346
TGGTATC14450.022.1688482
CGCAGAG80300.021.5221428
ACGCAGA80800.021.388967
AGAGTAC74850.020.54122510-11
AAGGTAA7050.020.0520881
TACATGG43200.019.8467662
GGCGTGC3800.019.8276568
GTACATG44500.019.6961061
GCAGAGT85300.019.653329
Produced by FastQC (version 0.11.5)