FastQCFastQC Report
Fri 27 May 2016
SRR939724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112315
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.4759443143354176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38600.3470239994965455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28140.25298588978841424No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA14470.13008904851593298No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA11720.1053658361165677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.054.6707341
GTATCAA40300.044.372641
TATCAAC51200.034.3746532
ATCAACG51650.033.8959963
TCAACGC52950.033.06384
CAACGCA53650.032.633875
AACGCAG55200.031.8027826
CGCAGAG64150.027.0722948
ACGCAGA64500.027.0713257
TGGTATC9350.026.1714882
GTCTACG909.4488E-426.1435281
TACATGG46450.025.428732
GTACATG48100.024.9477731
TACCGTA950.0012941424.7708997
ACATGGG47850.024.490253
GCAGAGT70600.024.4656499
CATGGGA23300.022.0164054
GTGGTAT13350.021.5023171
GGACCGT1100.003035161221.393056
GTACACG1100.003037540521.390161
Produced by FastQC (version 0.11.5)