FastQCFastQC Report
Fri 27 May 2016
SRR939724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112315
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63760.5732189173031021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53910.48466486561810285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43890.39458246989387No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.11966034801292799No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.11885122469803967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30550.049.082591
GTATCAA51950.038.7286071
GCGTATC651.3361334E-436.501291
TATCAAC70350.028.5240172
ATCAACG71850.027.9907743
TCAACGC72600.027.7016164
CAACGCA74100.027.1408545
CGTATCA909.014873E-426.3549332
AACGCAG76950.026.1356356
ACGCAGA89950.022.2001937
CGCAGAG90050.022.017528
TGGTATC15350.021.6333662
GCAGAGT95850.020.239849
AGAGTAC84150.020.23560510-11
GAGTACT60550.020.05403112-13
GTGGTAT17300.020.0229641
GTAAGGT5700.019.9715714
AGTACTT63650.019.59896712-13
TAAGGTG5850.019.459485
AGGTAAG5650.019.3114032
Produced by FastQC (version 0.11.5)