FastQCFastQC Report
Fri 27 May 2016
SRR939723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050683
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173050.5672500223720394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114380.3749324331633277No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96110.3150442048551095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82790.2713818512116795No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48980.1605542103194596No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40930.13416667677369296No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37270.12216936338518292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34660.1136139021982946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32020.10496010237707426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG171500.030.4294641
TACATGG176000.030.297882
ACATGGG179000.028.5462993
GTATCAA260050.024.756471
CATGGGA121850.024.6470724
CATGGGG54600.024.3305844
GAGTACT134600.022.25712812-13
TCAACGC304700.021.799314
CAACGCA304550.021.7788895
ATCAACG305400.021.6095263
TATCAAC305500.021.6010342
AACGCAG309250.021.509266
AGTACTT140150.021.1387912-13
GAGTACA134400.021.0031011
ATGGGAT49550.020.5864735
AGTACAT127950.020.2076032
GTACTTT149350.020.20407714-15
AGAGTAC223800.020.0735210-11
ATGGGGG28800.019.7685995
GGTATCA189600.019.6675381
Produced by FastQC (version 0.11.5)