##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939723_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3050683 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.021101176359522 31.0 30.0 33.0 16.0 34.0 2 29.81774376426525 31.0 30.0 33.0 25.0 34.0 3 30.091032401596625 31.0 30.0 34.0 25.0 34.0 4 33.86812133545177 35.0 35.0 37.0 28.0 37.0 5 33.86308246382859 35.0 35.0 37.0 28.0 37.0 6 33.91424739968066 35.0 35.0 37.0 29.0 37.0 7 33.83866563651484 35.0 35.0 37.0 28.0 37.0 8 33.70765136856238 35.0 33.0 37.0 28.0 37.0 9 34.96400117613007 37.0 34.0 39.0 28.0 39.0 10-11 34.813435057001996 37.0 34.0 39.0 27.0 39.0 12-13 34.70636378804353 37.0 34.0 39.0 27.0 39.0 14-15 35.344398614998674 38.0 34.0 40.0 27.0 41.0 16-17 35.2093311563345 38.0 33.5 40.0 27.0 41.0 18-19 35.11752056834486 38.0 33.5 40.0 27.0 41.0 20-21 35.32950145918144 38.0 34.0 40.0 27.0 41.0 22-23 35.238799967089335 38.0 34.0 40.0 26.5 41.0 24-25 35.01533099309236 38.0 34.0 40.0 25.5 41.0 26-27 34.896010991636956 38.0 33.5 40.0 25.0 41.0 28-29 34.93703819767573 38.0 34.0 40.0 25.0 41.0 30-31 34.70416739464572 38.0 33.5 40.0 24.5 41.0 32-33 34.331827003985666 38.0 33.0 40.0 24.0 41.0 34-35 34.12632925151515 38.0 33.0 40.0 23.5 41.0 36-37 33.88700038647083 38.0 33.0 40.0 22.5 41.0 38-39 33.64793261050066 38.0 32.5 40.0 20.5 41.0 40-41 33.4124677981947 37.0 32.0 40.0 19.0 41.0 42-43 32.99535792476635 37.0 31.0 40.0 16.5 41.0 44-45 32.609827373083334 37.0 31.0 40.0 15.0 41.0 46-47 32.17533155690054 37.0 30.5 40.0 11.0 41.0 48-49 31.78077696043804 36.5 30.0 39.0 8.5 41.0 50-51 31.138661571851287 35.5 29.5 38.5 8.0 40.0 52-53 31.310533247800574 36.0 30.0 39.0 7.5 40.0 54-55 31.421965179600768 36.0 30.0 39.0 4.0 40.0 56-57 31.115103404712976 36.0 29.5 39.0 2.0 40.0 58-59 30.729949653897176 35.5 28.5 39.0 2.0 40.0 60-61 30.308626133885426 35.0 28.0 39.0 2.0 40.0 62-63 29.888759009048137 35.0 28.0 38.0 2.0 40.0 64-65 29.36237180329782 34.0 26.5 38.0 2.0 40.0 66-67 28.753459799002385 34.0 26.0 38.0 2.0 40.0 68-69 28.178829789919178 34.0 25.5 37.0 2.0 39.0 70-71 27.589151019624133 33.0 24.5 36.0 2.0 39.0 72-73 26.92833358956011 32.5 22.5 36.0 2.0 39.0 74-75 26.55916461985726 32.0 22.0 35.5 2.0 38.0 76-77 25.989490550148936 32.0 20.5 35.0 2.0 37.5 78-79 25.304401506154523 31.0 18.5 35.0 2.0 37.0 80-81 24.539264158222927 31.0 13.0 34.5 2.0 36.0 82-83 23.764857738414644 30.5 7.5 34.0 2.0 36.0 84-85 22.95525559358347 30.0 2.0 34.0 2.0 35.0 86-87 21.989859647823124 29.0 2.0 34.0 2.0 35.0 88-89 20.93594958899368 28.5 2.0 34.0 2.0 35.0 90-91 19.491462731460462 26.0 2.0 33.0 2.0 34.5 92-93 17.906677783302953 22.0 2.0 32.0 2.0 34.0 94-95 17.397411825483015 20.0 2.0 32.0 2.0 34.0 96-97 17.12964326349214 20.0 2.0 32.0 2.0 34.0 98-99 16.271709646659453 10.0 2.0 32.0 2.0 34.0 100-101 14.634388922087282 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5164583554145707 1101 2 0.8040178921278951 1101 3 0.713743573431362 1101 4 0.6061270651835358 1101 5 0.7272299099145414 1101 6 0.9037991175941684 1101 7 0.7042403333745639 1101 8 0.9162617048237891 1101 9 0.8807448902876232 1101 10-11 0.8222772909648199 1101 12-13 0.8113944938292192 1101 14-15 0.9010166848491963 1101 16-17 0.633719607216392 1101 18-19 0.7713694303635279 1101 20-21 0.8035474962455922 1101 22-23 0.9709306522581826 1101 24-25 1.1061287195657812 1101 26-27 0.895182094128387 1101 28-29 0.8799470301610555 1101 30-31 0.9304197029888144 1101 32-33 1.0764619011291714 1101 34-35 1.0439914234899348 1101 36-37 0.808390126127442 1101 38-39 0.8743692768239058 1101 40-41 0.8343111140374901 1101 42-43 0.8343933515748319 1101 44-45 0.677233568128635 1101 46-47 0.6325808339996186 1101 48-49 0.6829388669603595 1101 50-51 0.7607781393331869 1101 52-53 0.6185306581733023 1101 54-55 0.6669489221167346 1101 56-57 0.7696451103066266 1101 58-59 0.5339765713256206 1101 60-61 0.5065636073040061 1101 62-63 0.12575118769730764 1101 64-65 0.4757325287603429 1101 66-67 0.48642181531346296 1101 68-69 0.39399021094892106 1101 70-71 0.3075486326224741 1101 72-73 0.33385159502424955 1101 74-75 0.24935094145597247 1101 76-77 0.08403962033480639 1101 78-79 -0.15929186570892284 1101 80-81 -0.2689367824333324 1101 82-83 -0.42027029146300166 1101 84-85 -0.23509113825577543 1101 86-87 -0.5400095203650785 1101 88-89 -4.4481283338111375 1101 90-91 -7.733882795652974 1101 92-93 -10.21872931950927 1101 94-95 -8.174110680860691 1101 96-97 -4.63449061311672 1101 98-99 -3.138680809529294 1101 100-101 -2.248027535174314 1102 1 1.9231046882255036 1102 2 1.2753457711466964 1102 3 1.0175414778947527 1102 4 1.036098600092835 1102 5 0.966031947117699 1102 6 1.088336881585306 1102 7 0.9750106150269957 1102 8 1.1937238156076475 1102 9 1.4506644914302527 1102 10-11 1.4110627625989949 1102 12-13 1.3532290841845551 1102 14-15 1.7240845306865182 1102 16-17 1.6670560449551814 1102 18-19 1.6119102545013746 1102 20-21 1.5518477555997876 1102 22-23 1.5870685054800049 1102 24-25 1.6301894612439085 1102 26-27 1.655623751390067 1102 28-29 1.4369815132885648 1102 30-31 1.671009342853658 1102 32-33 1.7941374104220884 1102 34-35 1.871744037404575 1102 36-37 1.8552444142500448 1102 38-39 1.819921413687915 1102 40-41 1.748759782640839 1102 42-43 1.8895684951037666 1102 44-45 1.886291205925687 1102 46-47 1.9419011454008412 1102 48-49 1.8361087113950312 1102 50-51 1.7648425244925754 1102 52-53 1.7346675674880565 1102 54-55 1.7906204122533786 1102 56-57 1.7587317354896328 1102 58-59 1.7848903520226536 1102 60-61 1.7892926408470053 1102 62-63 1.815664266442127 1102 64-65 1.697281596667846 1102 66-67 1.8001745569701875 1102 68-69 1.72013385661376 1102 70-71 1.6699786615783943 1102 72-73 1.8069125660717873 1102 74-75 1.6022750396864893 1102 76-77 1.5254288124101407 1102 78-79 1.4437937979474178 1102 80-81 1.3574386868064678 1102 82-83 1.1820094328783775 1102 84-85 1.2526841640383282 1102 86-87 1.1013795773195447 1102 88-89 0.6306293838089942 1102 90-91 -5.313023896859004 1102 92-93 -10.233206966059495 1102 94-95 -7.8214268069780015 1102 96-97 -4.099475122028618 1102 98-99 -2.5769343423374593 1102 100-101 -1.7364988116103994 1103 1 2.0201628685286614 1103 2 1.352359501530298 1103 3 1.078389860483341 1103 4 1.0156487072958527 1103 5 1.0792928474754078 1103 6 0.8822799687092697 1103 7 0.8871132521705007 1103 8 0.9368037188827003 1103 9 1.1706346120780609 1103 10-11 1.022286355752584 1103 12-13 0.9893295680399703 1103 14-15 1.0053430807443533 1103 16-17 1.003672081437891 1103 18-19 0.805631726433198 1103 20-21 0.7866249615666163 1103 22-23 0.9093165230200455 1103 24-25 0.8018348857028101 1103 26-27 0.8943784500289311 1103 28-29 0.6581602920277163 1103 30-31 0.9752253605118213 1103 32-33 0.8635863149304299 1103 34-35 1.0502065541817203 1103 36-37 0.9996583803095689 1103 38-39 1.0592296024113637 1103 40-41 0.7280301746297013 1103 42-43 0.8910482750965087 1103 44-45 0.8153131531311502 1103 46-47 1.0378540757320351 1103 48-49 0.9190676877273241 1103 50-51 0.9190011342076083 1103 52-53 0.8910630914909667 1103 54-55 0.91669861347577 1103 56-57 0.9116113257604752 1103 58-59 1.0083606039347544 1103 60-61 1.0851110887170883 1103 62-63 0.7925261662540173 1103 64-65 0.8734841875343449 1103 66-67 1.097277733935222 1103 68-69 0.99664359208737 1103 70-71 0.6764663243495797 1103 72-73 0.79026968384931 1103 74-75 0.7555927735618653 1103 76-77 0.6930699838136221 1103 78-79 0.49786101864629373 1103 80-81 0.4047733064837473 1103 82-83 -0.09851332198313045 1103 84-85 -0.2583002812581441 1103 86-87 -0.15296690874221497 1103 88-89 -0.8761758278218075 1103 90-91 -6.529896041745257 1103 92-93 -9.837466400132753 1103 94-95 -8.58439694192877 1103 96-97 -5.363888760504144 1103 98-99 -3.941020373740308 1103 100-101 -2.9242045608942124 1104 1 2.144189426655405 1104 2 1.2466429566287118 1104 3 1.0901212344003106 1104 4 0.8465631671794824 1104 5 0.8573533140979279 1104 6 0.9110273682407808 1104 7 0.9155069053550662 1104 8 1.0491324480046842 1104 9 1.116908844588174 1104 10-11 1.1459514453165127 1104 12-13 1.0283297584885034 1104 14-15 1.0350697752936284 1104 16-17 1.0358967898529272 1104 18-19 1.0748482884515411 1104 20-21 1.1484547961951108 1104 22-23 1.2516937515925264 1104 24-25 1.2063400461104479 1104 26-27 1.1168175665051265 1104 28-29 1.1777893285824206 1104 30-31 1.237089389308899 1104 32-33 1.3054390624063004 1104 34-35 1.3127682384550354 1104 36-37 1.3320278138707735 1104 38-39 1.252110880179366 1104 40-41 1.305193506676332 1104 42-43 1.3422541999346578 1104 44-45 1.1984630912157002 1104 46-47 1.0279567526750455 1104 48-49 0.8681797116010621 1104 50-51 1.0094135261113415 1104 52-53 0.9743731106184548 1104 54-55 0.9163243073439169 1104 56-57 1.0248306673830427 1104 58-59 0.9370595158204331 1104 60-61 1.0000909184859985 1104 62-63 1.0289624629901475 1104 64-65 0.9614436692152815 1104 66-67 0.8839165002851672 1104 68-69 0.8053171849422469 1104 70-71 0.6793200028197255 1104 72-73 0.6765457421470238 1104 74-75 0.49045821419641555 1104 76-77 0.785450950528805 1104 78-79 0.5498107256742806 1104 80-81 0.33140153323869725 1104 82-83 0.04078020445871999 1104 84-85 -0.19725857499667399 1104 86-87 -0.017957687334384076 1104 88-89 -0.28181349791221777 1104 90-91 -3.31745089898952 1104 92-93 -7.955554180773817 1104 94-95 -5.449027383238157 1104 96-97 -2.947565166803269 1104 98-99 -2.1501639003198783 1104 100-101 -1.551535440431973 1105 1 1.9708669273275774 1105 2 1.1509941368569478 1105 3 0.9644969793000833 1105 4 0.8284918677375757 1105 5 0.8198459672260512 1105 6 0.9171296664066801 1105 7 0.8509676254005001 1105 8 0.8181285744020741 1105 9 0.9097884073855482 1105 10-11 0.6980913842242131 1105 12-13 0.660241054325688 1105 14-15 0.8135273852664611 1105 16-17 0.778496218344273 1105 18-19 0.6874961481165087 1105 20-21 0.8271621626751653 1105 22-23 0.7628866954265234 1105 24-25 0.7824206614106544 1105 26-27 0.6245636823804617 1105 28-29 0.7964415941423155 1105 30-31 0.8862412124472314 1105 32-33 0.8643648123318997 1105 34-35 0.7773053892403325 1105 36-37 0.8910542784545186 1105 38-39 0.9270903188412163 1105 40-41 0.7390476942323687 1105 42-43 0.7766229532367248 1105 44-45 0.6911190912394716 1105 46-47 0.6624570193163635 1105 48-49 0.5398460638438998 1105 50-51 0.42523062948053436 1105 52-53 0.25402874967375766 1105 54-55 0.32966791020532327 1105 56-57 0.2561836752307656 1105 58-59 0.20651237378454468 1105 60-61 0.22136602349613455 1105 62-63 0.1559525554208534 1105 64-65 0.2782337039108356 1105 66-67 0.17165372316512162 1105 68-69 0.28217915584987097 1105 70-71 0.2763974951732884 1105 72-73 0.23837127207497133 1105 74-75 0.29545275015387773 1105 76-77 0.09711983796854184 1105 78-79 -0.11440952549837391 1105 80-81 0.019551567379679113 1105 82-83 -0.2760716751768655 1105 84-85 -0.24552109859703108 1105 86-87 0.12937067562658555 1105 88-89 -0.33746775449295896 1105 90-91 -3.2426012406221574 1105 92-93 -7.782115765509554 1105 94-95 -5.462267300507552 1105 96-97 -2.7558556072788747 1105 98-99 -1.9231664795856318 1105 100-101 -1.4287130235475622 1106 1 1.6657888448188878 1106 2 0.9690245854349477 1106 3 0.7431995211756899 1106 4 0.7009740075031985 1106 5 0.7006264705759406 1106 6 0.7519927826288111 1106 7 0.8335254959304592 1106 8 -0.21435644567584689 1106 9 -0.6131941866420973 1106 10-11 -0.6110727583389917 1106 12-13 -0.3143673074660498 1106 14-15 0.5810137152736488 1106 16-17 0.7002692344142787 1106 18-19 0.9873980949318479 1106 20-21 0.6826693044707923 1106 22-23 0.6764299851415103 1106 24-25 0.6422813646052035 1106 26-27 0.7489796914013596 1106 28-29 0.5875382117670753 1106 30-31 0.6571420914021004 1106 32-33 0.7821786246550388 1106 34-35 0.9110776637831179 1106 36-37 0.7546004025193795 1106 38-39 0.6885089952151517 1106 40-41 0.6673714290781732 1106 42-43 0.7760280461804143 1106 44-45 0.8393819411859198 1106 46-47 0.7850720671096312 1106 48-49 0.567667582534515 1106 50-51 0.5439416284433101 1106 52-53 0.5955766417601005 1106 54-55 0.7356339868675938 1106 56-57 0.7739038774041731 1106 58-59 0.6274660329302257 1106 60-61 0.34204130352224027 1106 62-63 0.6173173025432206 1106 64-65 0.6118670187059898 1106 66-67 0.46857163579667116 1106 68-69 0.323238620510125 1106 70-71 0.3018323380660384 1106 72-73 0.40834677409961984 1106 74-75 0.11651273572167753 1106 76-77 0.08590010231380063 1106 78-79 -0.05962261498569532 1106 80-81 -0.25328770046944626 1106 82-83 -0.40255246977536174 1106 84-85 -0.2707109617362349 1106 86-87 -0.06342793079252473 1106 88-89 -0.44136736028851686 1106 90-91 -3.6986732606862915 1106 92-93 -8.501490615174104 1106 94-95 -6.042786737253291 1106 96-97 -3.367249846960693 1106 98-99 -2.269907819880741 1106 100-101 -1.6052564036553285 1107 1 1.8018957188184999 1107 2 1.0973144831772927 1107 3 0.8819413663208522 1107 4 0.8579302086008198 1107 5 0.5892761946793783 1107 6 0.7046361265703922 1107 7 0.6369558776275213 1107 8 0.5868941690983505 1107 9 0.5795181641499667 1107 10-11 0.6632859671338167 1107 12-13 0.5953704520994947 1107 14-15 0.7443711243258591 1107 16-17 0.7915271326588922 1107 18-19 0.7467279304548384 1107 20-21 0.5661159773671045 1107 22-23 0.45787916551527275 1107 24-25 0.625452298152176 1107 26-27 0.6082473927650724 1107 28-29 0.599997601677785 1107 30-31 0.7550112528475665 1107 32-33 0.9103917421842453 1107 34-35 0.6627462641548902 1107 36-37 0.47228118163264554 1107 38-39 0.5844910268195918 1107 40-41 0.47108732768407435 1107 42-43 0.594748644404774 1107 44-45 0.41827178450256497 1107 46-47 0.3681536333570108 1107 48-49 0.5157592392435966 1107 50-51 0.5396547163051331 1107 52-53 0.39288976029343203 1107 54-55 0.3866496105759829 1107 56-57 0.4347159260351603 1107 58-59 0.5497696300071375 1107 60-61 0.2108836090342585 1107 62-63 0.25999314469892454 1107 64-65 0.26586408807523654 1107 66-67 0.46127355989663954 1107 68-69 0.47695712071769236 1107 70-71 0.5886526341293212 1107 72-73 0.5622986070421021 1107 74-75 0.4881529040863839 1107 76-77 0.42155398319857795 1107 78-79 0.2842762132387122 1107 80-81 0.10752260361772414 1107 82-83 0.06504830881860002 1107 84-85 0.13159575117707334 1107 86-87 0.29662917664632005 1107 88-89 0.20483311964359885 1107 90-91 -2.9496310101679732 1107 92-93 -4.329434728104511 1107 94-95 -2.50850929129383 1107 96-97 -1.0894145600932852 1107 98-99 -0.562533744755207 1107 100-101 -0.2853123933690114 1108 1 1.6651445096739366 1108 2 1.0382460615590716 1108 3 -0.42363431346530334 1108 4 -0.1817620008513714 1108 5 0.4132998049239589 1108 6 0.49922381465724897 1108 7 0.4758625738853013 1108 8 0.5064954113115832 1108 9 0.7527538294660872 1108 10-11 0.5903304026322331 1108 12-13 0.5803515093281462 1108 14-15 0.4451022268643712 1108 16-17 0.616497226213184 1108 18-19 0.6238531859203889 1108 20-21 0.3526027876125326 1108 22-23 0.448823747121601 1108 24-25 0.4105196261210651 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0.573272579890407 2311 78-79 0.5171443266838658 2311 80-81 0.7714846082995415 2311 82-83 1.0093088371064916 2311 84-85 1.1477870256967222 2311 86-87 1.4415355789887734 2311 88-89 1.9130927362910803 2311 90-91 2.428081699490008 2311 92-93 3.3543342096798945 2311 94-95 2.8031481173296697 2311 96-97 2.0888478378300306 2311 98-99 1.9100359223415246 2311 100-101 1.9094985957608372 2312 1 1.2932082083375889 2312 2 0.5888019872698393 2312 3 -0.117009211693059 2312 4 -0.5574139778542531 2312 5 -0.06354413522641522 2312 6 -0.2967726409277418 2312 7 0.0838592509502405 2312 8 0.06197089601971584 2312 9 0.13305532180681467 2312 10-11 0.11679297969493518 2312 12-13 0.17201518981720199 2312 14-15 0.3212584202641935 2312 16-17 0.01867142718379 2312 18-19 -0.2923076394272144 2312 20-21 0.2748158653045323 2312 22-23 0.3834936463408596 2312 24-25 0.2889867806813342 2312 26-27 0.3617939333325211 2312 28-29 0.23887698935303092 2312 30-31 0.24953356288021666 2312 32-33 0.5228279583558546 2312 34-35 0.39794488905939573 2312 36-37 0.32284335772259 2312 38-39 0.45630516267349464 2312 40-41 0.38044075863517435 2312 42-43 0.6428320684617574 2312 44-45 0.46645669039396154 2312 46-47 0.5800220836196033 2312 48-49 0.545244203961186 2312 50-51 0.39753988811221674 2312 52-53 0.3116680254797721 2312 54-55 0.30259669894569896 2312 56-57 0.5102473581201892 2312 58-59 0.46324655070889875 2312 60-61 0.4324211153893529 2312 62-63 0.5836762764882266 2312 64-65 0.49062971494683083 2312 66-67 0.6066501140659781 2312 68-69 0.6011053387632259 2312 70-71 0.6632321333347697 2312 72-73 0.5007962899290064 2312 74-75 0.5195034709206539 2312 76-77 0.603640675968812 2312 78-79 0.8333794465497739 2312 80-81 0.946339172373829 2312 82-83 0.8880040986733455 2312 84-85 1.0920035284786813 2312 86-87 1.2894016736717369 2312 88-89 1.7695815245488014 2312 90-91 2.568639380028017 2312 92-93 3.749014389901891 2312 94-95 3.3054911425826035 2312 96-97 2.618148731298664 2312 98-99 2.4577739348576593 2312 100-101 2.349184642695967 2313 1 -7.422737119674284 2313 2 -2.7510760880653073 2313 3 -1.603241294024798 2313 4 -0.7778449903547155 2313 5 -0.5933158291068352 2313 6 -0.0905826470304163 2313 7 -0.18459884827990436 2313 8 -0.09488838707299863 2313 9 -0.056187200977952045 2313 10-11 -0.18084266167378615 2313 12-13 -0.24317940830844265 2313 14-15 -0.48753860689989637 2313 16-17 -0.6260577055194005 2313 18-19 -0.6933299815382838 2313 20-21 -0.721813335301718 2313 22-23 -1.0861478498757648 2313 24-25 -0.46132336724418366 2313 26-27 -0.5365244632385142 2313 28-29 -0.6535772477117234 2313 30-31 -0.22833186730139943 2313 32-33 -0.37138151540978015 2313 34-35 -0.12702452591422997 2313 36-37 -0.12765187617048923 2313 38-39 -0.194033493075203 2313 40-41 -0.3950424059910631 2313 42-43 -0.25943355139000346 2313 44-45 -0.3046396359647474 2313 46-47 -1.3180176812123463 2313 48-49 -0.8118318603547543 2313 50-51 -0.7234337400025161 2313 52-53 -1.0336709454307886 2313 54-55 -0.6796562857804105 2313 56-57 -0.2942419793809208 2313 58-59 -0.32509826215657966 2313 60-61 -0.25527854681443074 2313 62-63 -0.20106655859330047 2313 64-65 -0.384512286961332 2313 66-67 -0.14454051731783935 2313 68-69 -0.1888985506206886 2313 70-71 -0.21424152916125294 2313 72-73 -0.21289831679196425 2313 74-75 -0.10458112711904022 2313 76-77 0.17785556478830244 2313 78-79 -0.06860592993972148 2313 80-81 0.06246664961771131 2313 82-83 0.40069831717219984 2313 84-85 0.584255739625668 2313 86-87 1.103437908690804 2313 88-89 1.7282598646199112 2313 90-91 2.4325902706467915 2313 92-93 3.2888085892045247 2313 94-95 2.874479358393984 2313 96-97 1.9593361609244475 2313 98-99 1.7061837999970315 2313 100-101 1.4609637815591512 2314 1 0.6221781927004706 2314 2 -0.0644003526676471 2314 3 -1.2604895690402351 2314 4 -0.21572126446027085 2314 5 -0.1574403926703951 2314 6 -3.781084580529601 2314 7 -2.717509405438186 2314 8 -2.5972364783067157 2314 9 -3.203658667873775 2314 10-11 -2.458884537908176 2314 12-13 -1.236876309293784 2314 14-15 -1.2831233324368938 2314 16-17 -2.1371747842205835 2314 18-19 -3.0799759291334183 2314 20-21 -3.0089522258956336 2314 22-23 -3.9469536460405408 2314 24-25 -3.0405658724525395 2314 26-27 -1.850113658751205 2314 28-29 -1.83770125162566 2314 30-31 -1.9015294795833597 2314 32-33 -2.2069294665505623 2314 34-35 -1.6918836747413124 2314 36-37 -1.9525481604580577 2314 38-39 -3.222223586193678 2314 40-41 -2.543563351329542 2314 42-43 -1.8150855161212434 2314 44-45 -2.171216502937831 2314 46-47 -1.7194530922298625 2314 48-49 -2.250666353907949 2314 50-51 -3.1626628768061593 2314 52-53 -2.0109785974659253 2314 54-55 -2.375083722693457 2314 56-57 -1.4005695405000793 2314 58-59 -0.8825573987074797 2314 60-61 -1.2997563395563176 2314 62-63 -1.1068098725856252 2314 64-65 -1.7651667791424863 2314 66-67 -1.024783215695578 2314 68-69 -0.995301438773808 2314 70-71 -0.9779800247508526 2314 72-73 -2.1531489946901416 2314 74-75 -2.125902694571259 2314 76-77 -1.25873820901937 2314 78-79 -2.0864952204123206 2314 80-81 -0.9866487429133137 2314 82-83 -0.670753504216524 2314 84-85 -1.7864804975069006 2314 86-87 -0.5993053673897712 2314 88-89 0.5401757077591505 2314 90-91 1.5227844419862322 2314 92-93 2.434407215716032 2314 94-95 2.1643230717233948 2314 96-97 1.604112012223741 2314 98-99 1.487560826921591 2314 100-101 1.3927102223680805 2315 1 -3.0840250046968407 2315 2 -2.1162994890573685 2315 3 -3.0361519785176583 2315 4 -4.193814682336669 2315 5 -4.018013571903907 2315 6 -3.406100823611471 2315 7 -5.962852976260393 2315 8 -3.2066418155225804 2315 9 -2.6454335031902687 2315 10-11 -3.8833198240472555 2315 12-13 -3.1060571362919234 2315 14-15 -2.3806154954462926 2315 16-17 -2.6794672640293697 2315 18-19 -2.1331192618818378 2315 20-21 -1.4842193475266754 2315 22-23 -0.8859446305372884 2315 24-25 -2.459624105679488 2315 26-27 -4.1036577332275215 2315 28-29 -3.371641230474083 2315 30-31 -3.503565200135956 2315 32-33 -4.302871067335921 2315 34-35 -4.358637524470282 2315 36-37 -3.327650005767005 2315 38-39 -2.098447271215427 2315 40-41 -1.7104435888342238 2315 42-43 -3.249299611796552 2315 44-45 -6.636450616729515 2315 46-47 -3.397351593123826 2315 48-49 -2.3379298807197024 2315 50-51 -3.514630779527881 2315 52-53 -3.354839423894962 2315 54-55 -1.9182229768870158 2315 56-57 -1.5034351995423734 2315 58-59 -2.890460073006416 2315 60-61 -3.088189605387985 2315 62-63 -4.8682981182853595 2315 64-65 -4.036774820535076 2315 66-67 -3.6796551917554083 2315 68-69 -2.13019229000615 2315 70-71 -3.6294101273312513 2315 72-73 -2.4978053764428765 2315 74-75 -1.502075160531927 2315 76-77 -3.1724423904866086 2315 78-79 -1.9117834461935281 2315 80-81 -1.53403419954547 2315 82-83 -1.123316354272724 2315 84-85 -2.6577798017813237 2315 86-87 -1.9619811365056243 2315 88-89 -1.3224647296895746 2315 90-91 -0.6419687817319861 2315 92-93 -0.17552169412419616 2315 94-95 -0.1385885896237582 2315 96-97 -0.4897471543283167 2315 98-99 -0.7242499091627081 2315 100-101 -1.150409113301727 2316 1 -7.713503337435167 2316 2 -6.289974047324964 2316 3 -3.586261280695389 2316 4 -4.215224215833999 2316 5 -2.35101793910054 2316 6 -2.999548072473214 2316 7 -3.533977150380963 2316 8 -2.502995964359382 2316 9 -7.088515677887912 2316 10-11 -7.0090231970097605 2316 12-13 -6.103250002062982 2316 14-15 -5.176259057865906 2316 16-17 -4.762892212312565 2316 18-19 -4.096085874590237 2316 20-21 -2.994091510438267 2316 22-23 -3.6508080150413207 2316 24-25 -3.210380907111414 2316 26-27 -4.177843590943958 2316 28-29 -4.329315857208368 2316 30-31 -6.709355281268984 2316 32-33 -4.860135382986918 2316 34-35 -5.766141229007175 2316 36-37 -4.453714785035295 2316 38-39 -6.610734352040353 2316 40-41 -3.93367335815039 2316 42-43 -4.723453681407612 2316 44-45 -3.3321191205856557 2316 46-47 -4.320483063837202 2316 48-49 -5.418371882098821 2316 50-51 -4.819137843822997 2316 52-53 -6.1112542635606495 2316 54-55 -5.052995740838327 2316 56-57 -4.446697588345138 2316 58-59 -3.0653721666688476 2316 60-61 -3.2260812715248512 2316 62-63 -4.15930868660778 2316 64-65 -3.1929981979968183 2316 66-67 -4.15666725656402 2316 68-69 -3.2660270212010403 2316 70-71 -2.7323209396885098 2316 72-73 -2.311767690421327 2316 74-75 -2.443962836038949 2316 76-77 -2.947218528867566 2316 78-79 -5.054424255274167 2316 80-81 -5.962965532347372 2316 82-83 -3.5982514331805824 2316 84-85 -2.2602700330520165 2316 86-87 -2.309794603903253 2316 88-89 -1.287246932262942 2316 90-91 -1.1267950964146678 2316 92-93 -1.1504325651671863 2316 94-95 -1.8198763991298712 2316 96-97 -3.509056472155576 2316 98-99 -4.745628143602431 2316 100-101 -4.1564522766583565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21969.0 3 7079.0 4 6875.0 5 7530.0 6 8613.0 7 10424.0 8 12581.0 9 15479.0 10 18492.0 11 22475.0 12 27277.0 13 31237.0 14 34838.0 15 39704.0 16 44906.0 17 48281.0 18 43395.0 19 41046.0 20 43870.0 21 48253.0 22 53953.0 23 60681.0 24 68470.0 25 77787.0 26 88090.0 27 100679.0 28 114176.0 29 130301.0 30 147680.0 31 166484.0 32 187165.0 33 207824.0 34 234418.0 35 271939.0 36 291729.0 37 238899.0 38 74658.0 39 1426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.747024875282236 19.785445069861314 12.660342622245402 21.807187432611048 2 15.590758426044435 21.18768665288307 39.59479505103213 23.626759870040367 3 19.854212239017098 26.384007325490376 29.410957712687836 24.35082272280469 4 12.250711689631439 16.525313437205718 38.13153120684771 33.09244366631513 5 12.736882248575473 39.04760717012069 33.83312908724644 14.382381494057391 6 30.43917246667376 36.92759805524154 18.12786143530317 14.505368042781535 7 28.011934362973662 33.968116588232625 21.036760845518163 16.98318820327555 8 26.752990428265832 33.736784532897005 20.319383910714592 19.190841128122564 9 27.550912864283035 16.51180024053244 20.908067423942203 35.02921947124232 10-11 24.852577067602216 26.956346593558877 27.435546019281244 20.755530319557664 12-13 26.370165939013717 24.8151021786256 27.832475080916137 20.98225680144455 14-15 22.86275335682039 25.812336500633847 27.099944535171417 24.224965607374344 16-17 21.392985176220826 29.30135884779188 27.57188785250444 21.733768123482854 18-19 21.283893650793388 27.674026026685965 30.49508028952394 20.547000032996706 20-21 22.264962870578028 26.557752163304116 30.287538163280146 20.889746802837706 22-23 21.614473379313154 26.808084421039048 30.32027396733853 21.257168232309272 24-25 21.531103072091476 26.97376904359813 30.63160510837482 20.863522775935568 26-27 21.254311667607475 27.012708453398503 30.856409393701828 20.876570485292195 28-29 20.754921862461043 27.748259936828916 30.685910732857817 20.81090746785222 30-31 21.02672490075713 27.152063546973864 30.569480848964076 21.25173070330493 32-33 20.801924774523645 27.359341947094656 30.43341922126595 21.40531405711575 34-35 21.708347136853007 27.146487686548713 30.373612345922744 20.771552830675528 36-37 21.533625989150654 26.790162493788934 30.447089936226217 21.229121580834192 38-39 20.94025495472014 27.407574931155132 30.53677072354985 21.115399390574886 40-41 21.125796596807042 27.315994349911303 30.51264700085408 21.045562052427567 42-43 20.905245576131435 27.358702676780243 30.024134298048317 21.71191744904001 44-45 21.08427215486618 27.25465379093072 30.079994639763008 21.58107941444009 46-47 21.557107815842976 27.699699391918237 29.473980204082373 21.269212588156417 48-49 21.28380754207807 27.742476751820718 29.340174970112066 21.633540735989147 50-51 20.84715473551308 27.895281148187994 29.645387786864614 21.612176329434313 52-53 21.38320993553431 28.203118928048614 28.908654518423237 21.505016617993835 54-55 20.694293892838147 27.769866116284497 29.402576720523477 22.13326327035388 56-57 21.08855829729523 27.93930814066376 29.080639825164127 21.89149373687688 58-59 21.529575978017874 27.445182259817713 29.007533645634958 22.01770811652945 60-61 20.97169232507482 27.84948906528771 29.627447270854518 21.551371338782953 62-63 20.881735798814333 27.661567706196884 29.592126951931263 21.86456954305752 64-65 20.545798736346494 27.909184839048063 29.320285389425067 22.22473103518038 66-67 20.767953223404263 27.797284995941173 28.826353022786737 22.608408757867824 68-69 20.885211683375307 27.482520181338494 29.048679088183682 22.583589047102517 70-71 21.046577284512026 27.65012000797535 28.857721816027627 22.445580891485 72-73 21.102594820085642 27.372292873083424 29.023265717041983 22.501846589788947 74-75 20.648706479096678 28.08450277068634 28.73149733708859 22.5352934131284 76-77 20.934597989966264 27.551567007368543 28.741191889143835 22.772643113521358 78-79 21.054293696899354 27.645674952892534 28.653909476109764 22.646121874098352 80-81 21.166872629510095 27.744591832039493 28.446832006625865 22.641703531824543 82-83 21.233232839100662 27.697675267014148 28.33260315339136 22.73648874049383 84-85 20.82731074392711 27.716999687520843 28.424365751101643 23.0313238174504 86-87 20.83932118134936 27.561970633024856 28.58218985767665 23.016518327949132 88-89 21.05897099417899 27.433972041596355 28.18213199627563 23.32492496794903 90-91 20.995441939000564 27.299922915750024 28.41133196086168 23.29330318438773 92-93 21.669857391574972 27.071732133396587 28.40324850631546 22.855161968712984 94-95 21.189031785208563 26.716535941940755 28.687857244225096 23.406575028625582 96-97 21.25883490664961 26.72710879056365 28.39774517995894 23.616311122827803 98-99 21.389748540419113 26.92869196036937 27.72368512772895 23.95787437148257 100-101 21.51482294161987 26.708032250553494 27.99055280837388 23.786591999452757 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5487.0 1 3489.0 2 1734.0 3 2024.5 4 1871.0 5 1820.5 6 2578.5 7 4427.0 8 6656.5 9 7363.0 10 6311.0 11 5328.5 12 5007.0 13 4963.5 14 5200.0 15 5375.5 16 5442.0 17 5788.0 18 6174.5 19 6702.0 20 7550.5 21 8638.0 22 10144.5 23 11946.5 24 13956.0 25 16599.0 26 20681.0 27 25743.0 28 29855.5 29 34347.0 30 40033.0 31 46326.0 32 54544.5 33 61444.5 34 67490.0 35 73398.0 36 80184.0 37 87416.5 38 90584.5 39 91377.0 40 92653.0 41 95854.5 42 98881.0 43 101248.0 44 103547.5 45 106173.5 46 109127.0 47 114528.0 48 124470.0 49 123803.0 50 112108.5 51 102408.5 52 95718.5 53 91817.5 54 86954.5 55 79615.0 56 72141.0 57 65145.5 58 58752.5 59 51639.5 60 43804.5 61 36704.5 62 29848.5 63 22621.5 64 16445.0 65 12535.5 66 9502.0 67 7235.5 68 5294.5 69 3684.0 70 2852.5 71 2253.0 72 1825.5 73 1579.0 74 1278.0 75 1037.5 76 754.0 77 498.5 78 340.5 79 228.5 80 155.5 81 119.0 82 79.5 83 49.5 84 35.0 85 23.5 86 14.5 87 8.0 88 7.0 89 7.5 90 4.0 91 1.0 92 0.5 93 2.0 94 4.0 95 2.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13124929728850884 2 0.11744910893724454 3 0.12416891561660126 4 0.13308495179604043 5 0.12144821340008122 6 0.13554341765434166 7 0.1646844329614057 8 0.1518020718639072 9 0.1619309512001083 10-11 0.15634203881557013 12-13 0.1466392935614746 14-15 0.15106453210641682 16-17 0.14627871856892374 18-19 0.1614228682560594 20-21 0.16561864998756018 22-23 0.19951269928733992 24-25 0.22222892381804338 26-27 0.23591438376258694 28-29 0.23088272363926376 30-31 0.22163889201205106 32-33 0.22270422721731492 34-35 0.21213282402661962 36-37 0.22029493067617975 38-39 0.2077731445712321 40-41 0.21250978879155913 42-43 0.2052163400785988 44-45 0.19803761977235917 46-47 0.21372263194832106 48-49 0.25073073800194906 50-51 0.2523041561512619 52-53 0.25392674361774065 54-55 0.2526155618266467 56-57 0.22663777259059695 58-59 0.22740809189286468 60-61 0.1988734981641816 62-63 0.19058027333551208 64-65 0.19212091194004752 66-67 0.19941436065300785 68-69 0.1999716129142228 70-71 0.2065766911868588 72-73 0.23716000646412622 74-75 0.2394054052813747 76-77 0.25400869247968405 78-79 0.2504685016437303 80-81 0.2643014695397719 82-83 0.2640064536367758 84-85 0.23863508597910696 86-87 0.24147051660234775 88-89 0.24530572334129766 90-91 0.4293300877213398 92-93 0.5707574336632157 94-95 0.3321715170012748 96-97 0.278265555614923 98-99 0.3080949413623113 100-101 0.32750043187050243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3050683.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.862003554829016 #Duplication Level Percentage of deduplicated Percentage of total 1 75.37867838563241 36.831532512370835 2 12.715912398978293 12.426499136835433 3 4.756789492107033 6.972787952187214 4 2.368729053341766 4.629633896992487 5 1.2889622616783236 3.149063930608334 6 0.7966159269189917 2.335455015176951 7 0.5162780390455696 1.765846556538734 8 0.3678303680587483 1.4378343001328497 9 0.25797398688882195 1.1344613277973528 >10 1.3340087023199638 11.96251682573737 >50 0.12691130452347268 4.303911786491907 >100 0.08277921126856908 7.427584935215374 >500 0.005895316140281957 1.875725244637921 >1k 0.0023581264559912416 2.166798328547433 >5k 1.3871332094066128E-4 0.606275174351928 >10k+ 1.3871332094066128E-4 0.9740730763777232 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17305 0.5672500223720394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11438 0.3749324331633277 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9611 0.3150442048551095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8279 0.2713818512116795 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4898 0.1605542103194596 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4093 0.13416667677369296 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3727 0.12216936338518292 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3466 0.1136139021982946 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3202 0.10496010237707426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.277954477734986E-5 0.0 0.0 0.0 3.277954477734986E-5 6 6.555908955469972E-5 0.0 0.0 0.0 3.277954477734986E-5 7 9.833863433204958E-5 0.0 0.0 0.0 3.277954477734986E-5 8 9.833863433204958E-5 0.0 0.0 0.0 3.277954477734986E-5 9 9.833863433204958E-5 0.0 0.0 3.277954477734986E-5 3.277954477734986E-5 10-11 9.833863433204958E-5 0.0 0.0 4.9169317166024783E-5 3.277954477734986E-5 12-13 9.833863433204958E-5 0.0 0.0 9.833863433204958E-5 3.277954477734986E-5 14-15 1.3111817910939943E-4 0.0 0.0 9.833863433204958E-5 3.277954477734986E-5 16-17 1.3111817910939943E-4 0.0 0.0 1.3111817910939943E-4 3.277954477734986E-5 18-19 1.8028749627542424E-4 0.0 0.0 1.8028749627542424E-4 3.277954477734986E-5 20-21 2.2945681344144903E-4 0.0 0.0 3.1140567538482365E-4 3.277954477734986E-5 22-23 2.2945681344144903E-4 0.0 0.0 7.375397574903718E-4 3.277954477734986E-5 24-25 3.933545373281983E-4 0.0 0.0 0.0017373158731995425 3.277954477734986E-5 26-27 3.933545373281983E-4 0.0 0.0 0.003359903339678361 3.277954477734986E-5 28-29 4.425238544942231E-4 0.0 0.0 0.00970274525409556 3.277954477734986E-5 30-31 4.916931716602478E-4 0.0 0.0 0.030353858463825968 3.277954477734986E-5 32-33 4.916931716602478E-4 0.0 0.0 0.08024432561495246 3.277954477734986E-5 34-35 5.244727164375977E-4 0.0 0.0 0.1588660637634261 3.277954477734986E-5 36-37 5.572522612149476E-4 0.0 0.0 0.28901724630189374 3.277954477734986E-5 38-39 5.736420336036226E-4 0.0 0.0 0.49379106252599825 3.277954477734986E-5 40-41 5.900318059922975E-4 0.0 0.0 0.7183309442508448 3.277954477734986E-5 42-43 7.047602127130219E-4 0.0 0.0 0.9374622010874286 3.277954477734986E-5 44-45 8.194886194337464E-4 0.0 0.0 1.1993871536308427 3.277954477734986E-5 46-47 9.178272537657961E-4 0.0 0.0 1.5148738823404464 6.555908955469972E-5 48-49 9.506067985431459E-4 0.0 0.0 1.8469142811626118 6.555908955469972E-5 50-51 9.506067985431459E-4 0.0 0.0 2.207554832803015 6.555908955469972E-5 52-53 9.506067985431459E-4 0.0 0.0 2.554477144954097 6.555908955469972E-5 54-55 9.506067985431459E-4 1.638977238867493E-5 0.0 2.9154618818146627 6.555908955469972E-5 56-57 0.0010489454328751955 3.277954477734986E-5 0.0 3.3428579763941384 6.555908955469972E-5 58-59 0.0010489454328751955 3.277954477734986E-5 0.0 3.7941831386610803 6.555908955469972E-5 60-61 0.0010653352052638703 3.277954477734986E-5 0.0 4.285253498970558 6.555908955469972E-5 62-63 0.0011145045224298952 3.277954477734986E-5 0.0 4.7587212437346 6.555908955469972E-5 64-65 0.0011145045224298952 3.277954477734986E-5 0.0 5.212816277535227 6.555908955469972E-5 66-67 0.0011472840672072451 3.277954477734986E-5 0.0 5.723144620401399 6.555908955469972E-5 68-69 0.0011472840672072451 3.277954477734986E-5 0.0 6.2862808099038805 6.555908955469972E-5 70-71 0.0011636738395959202 3.277954477734986E-5 0.0 6.895800055266313 6.555908955469972E-5 72-73 0.0011964533843732699 3.277954477734986E-5 0.0 7.479702086385245 6.555908955469972E-5 74-75 0.0012128431567619447 3.277954477734986E-5 0.0 8.045427859925137 6.555908955469972E-5 76-77 0.0012128431567619447 3.277954477734986E-5 0.0 8.627576185398484 6.555908955469972E-5 78-79 0.0012620124739279696 3.277954477734986E-5 0.0 9.283625994572363 6.555908955469972E-5 80-81 0.0014750795149807436 3.277954477734986E-5 0.0 9.960277747638807 6.555908955469972E-5 82-83 0.0015078590597580935 3.277954477734986E-5 0.0 10.576877374673147 6.555908955469972E-5 84-85 0.0015242488321467683 3.277954477734986E-5 0.0 11.14791343446697 6.555908955469972E-5 86-87 0.0015570283769241185 3.277954477734986E-5 0.0 11.722374956690027 6.555908955469972E-5 88-89 0.0015734181493127933 3.277954477734986E-5 0.0 12.325403852186543 9.833863433204958E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17150 0.0 30.429464 1 TACATGG 17600 0.0 30.29788 2 ACATGGG 17900 0.0 28.546299 3 GTATCAA 26005 0.0 24.75647 1 CATGGGA 12185 0.0 24.647072 4 CATGGGG 5460 0.0 24.330584 4 GAGTACT 13460 0.0 22.257128 12-13 TCAACGC 30470 0.0 21.79931 4 CAACGCA 30455 0.0 21.778889 5 ATCAACG 30540 0.0 21.609526 3 TATCAAC 30550 0.0 21.601034 2 AACGCAG 30925 0.0 21.50926 6 AGTACTT 14015 0.0 21.13879 12-13 GAGTACA 13440 0.0 21.003101 1 ATGGGAT 4955 0.0 20.586473 5 AGTACAT 12795 0.0 20.207603 2 GTACTTT 14935 0.0 20.204077 14-15 AGAGTAC 22380 0.0 20.07352 10-11 ATGGGGG 2880 0.0 19.768599 5 GGTATCA 18960 0.0 19.667538 1 >>END_MODULE