FastQCFastQC Report
Fri 27 May 2016
SRR939723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050683
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186550.6115024078214616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140790.46150321092030866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113970.37358847182745636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107820.35342905178938616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54650.17914021220821696No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44100.14455779246811287No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42820.1403620107366121No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42820.1403620107366121No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40270.1320032268183879No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.10535345691440245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG178850.028.1904161
TACATGG182300.027.2402882
ACATGGG179800.026.5414853
GTATCAA260100.024.515971
CATGGGG53550.022.9830844
CATGGGA121000.022.9539384
ATGGGCG5450.021.6307095
GAGTACT139550.021.46115512-13
GGTATCA188100.021.0621761
AGTACTT145750.020.98473512-13
ATCAACG302600.020.9598313
TCAACGC303900.020.8698274
CAACGCA305350.020.7552815
AACGCAG306900.020.7297276
ATGGGAG45100.020.2839475
AGAGTAC232850.020.23893210-11
TATCAAC315650.020.1546922
GAGTACA140650.019.9186291
GTACTTT151900.019.74731314-15
ATGGGAT51850.019.4622155
Produced by FastQC (version 0.11.5)