##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939723_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3050683 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.393054932288933 31.0 31.0 34.0 30.0 34.0 2 31.600430788777462 31.0 31.0 34.0 30.0 34.0 3 31.65909011195198 31.0 31.0 34.0 30.0 34.0 4 35.46129243844739 37.0 35.0 37.0 33.0 37.0 5 35.207564011075554 37.0 35.0 37.0 32.0 37.0 6 35.242595838374555 37.0 35.0 37.0 32.0 37.0 7 35.16042473111759 37.0 35.0 37.0 32.0 37.0 8 35.10527347482515 37.0 35.0 37.0 32.0 37.0 9 36.68486925714668 39.0 35.0 39.0 32.0 39.0 10-11 36.545473095696934 38.5 35.0 39.0 32.0 39.0 12-13 36.48704617949488 38.0 35.0 39.0 32.0 39.0 14-15 37.475926866213236 39.0 36.0 41.0 32.0 41.0 16-17 37.426393696100185 39.0 36.0 41.0 32.0 41.0 18-19 37.44305717768775 39.0 36.0 41.0 32.0 41.0 20-21 37.38394828305661 39.0 36.0 41.0 32.0 41.0 22-23 37.237533693274585 39.0 36.0 40.0 31.0 41.0 24-25 37.065054612360576 39.0 36.0 40.0 31.0 41.0 26-27 36.764499785785674 39.0 35.5 40.0 30.5 41.0 28-29 36.54155626789149 38.0 35.0 40.0 30.0 41.0 30-31 36.258601270600714 38.0 35.0 40.0 30.0 41.0 32-33 36.17059294590752 38.0 35.0 40.0 30.0 41.0 34-35 36.17129852560886 38.0 35.0 40.0 30.0 41.0 36-37 36.09270268330076 38.0 35.0 40.0 30.0 41.0 38-39 35.942408634394326 38.0 35.0 40.0 29.5 41.0 40-41 35.7613601609869 38.0 35.0 40.0 29.0 41.0 42-43 35.515814983071 38.0 34.0 40.0 27.5 41.0 44-45 35.21678702769183 38.0 34.0 40.0 27.0 41.0 46-47 34.845125665301836 38.0 33.5 40.0 25.5 41.0 48-49 34.493546199326516 38.0 33.0 40.0 24.0 41.0 50-51 34.20875702260773 38.0 33.0 40.0 24.0 41.0 52-53 33.937896366158 38.0 33.0 40.0 23.0 41.0 54-55 33.63132649311646 37.0 33.0 40.0 22.5 41.0 56-57 32.254707224578894 36.5 30.5 39.5 14.0 41.0 58-59 28.78058585569199 34.0 23.0 38.5 6.5 40.0 60-61 28.770421574447425 34.0 23.0 38.0 6.5 40.0 62-63 30.7636168687471 35.0 29.0 38.0 4.0 40.0 64-65 30.36503973044725 35.0 29.0 38.0 2.0 40.0 66-67 24.90352012975455 28.0 17.5 36.0 2.0 39.0 68-69 17.277381819087726 13.0 7.0 29.0 2.0 35.5 70-71 16.139072955138243 12.5 7.5 25.5 2.0 33.5 72-73 18.19823495263192 17.0 7.0 30.0 2.0 34.5 74-75 18.903522096527237 17.5 7.5 30.5 2.0 35.0 76-77 19.873762859005673 22.5 7.5 31.0 2.0 34.5 78-79 22.870889731905937 27.5 10.5 34.0 2.0 36.0 80-81 25.46608775805287 31.5 20.0 34.5 2.0 36.0 82-83 26.494387978036393 32.5 25.0 35.0 2.0 36.0 84-85 26.490574733592446 33.0 25.0 35.0 2.0 36.0 86-87 26.20434719044883 33.0 24.0 35.0 2.0 36.0 88-89 25.744778956056727 33.0 21.5 35.0 2.0 35.0 90-91 24.044160602724048 31.5 10.0 34.5 2.0 35.0 92-93 21.873220521437332 30.5 2.0 34.0 2.0 35.0 94-95 17.512045827114783 20.0 2.0 33.0 2.0 35.0 96-97 16.207898198534558 12.0 2.0 32.5 2.0 34.5 98-99 15.13931912951952 2.0 2.0 31.5 2.0 34.0 100-101 14.529052182740717 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28983234966813853 1101 2 0.338745644832521 1101 3 0.34922595057102157 1101 4 0.295571536121912 1101 5 0.38502378479370236 1101 6 0.30367292236422827 1101 7 0.3161869104631734 1101 8 0.31579832294551835 1101 9 0.506344574212541 1101 10-11 0.4515356157439001 1101 12-13 0.4207112230706471 1101 14-15 0.30707116580230576 1101 16-17 0.4607676992844034 1101 18-19 0.4631149248476234 1101 20-21 0.6835216810966287 1101 22-23 0.42767682335018975 1101 24-25 0.4125612494200297 1101 26-27 0.48344326194713005 1101 28-29 0.34123075345808473 1101 30-31 0.3698785581751949 1101 32-33 0.4164356060557921 1101 34-35 0.6391144579191206 1101 36-37 0.6537577825210761 1101 38-39 0.5167264490590497 1101 40-41 0.5544382847893203 1101 42-43 0.5220047626806092 1101 44-45 0.3358301530739851 1101 46-47 0.3273597055431381 1101 48-49 0.3373280043592146 1101 50-51 0.27588080558346917 1101 52-53 0.3904957421888824 1101 54-55 0.2551050218695039 1101 56-57 0.7995148639254737 1101 58-59 -8.343396190620737 1101 60-61 -9.275363062396863 1101 62-63 -2.682008795426082 1101 64-65 -0.3143106974000709 1101 66-67 -1.381710755200288 1101 68-69 -3.838411369071732 1101 70-71 -4.343239439511379 1101 72-73 -6.739390975405428 1101 74-75 -7.589188283423834 1101 76-77 -8.761983426498427 1101 78-79 -11.509318180232693 1101 80-81 -7.717915490279598 1101 82-83 -3.940361507844397 1101 84-85 -3.0707146342278655 1101 86-87 -3.0449211145815376 1101 88-89 -3.199309894251126 1101 90-91 -8.658328244201215 1101 92-93 -13.961202949072876 1101 94-95 -12.265641628986103 1101 96-97 -12.068004958887013 1101 98-99 -11.630916399768168 1101 100-101 -11.477391308180021 1102 1 0.5912232911178954 1102 2 0.6029951018499808 1102 3 0.601848360372049 1102 4 0.46377168469287966 1102 5 0.5229138189914551 1102 6 0.526912291846898 1102 7 0.5771487391761312 1102 8 0.67573399882545 1102 9 0.7522013728132961 1102 10-11 0.6873187235201499 1102 12-13 0.6836096344436058 1102 14-15 0.8387547436128031 1102 16-17 0.8955609474542428 1102 18-19 0.9382225223870435 1102 20-21 0.9570628805948473 1102 22-23 0.9375172118119934 1102 24-25 0.9479797888245329 1102 26-27 1.1550079672483307 1102 28-29 1.0666717861679231 1102 30-31 1.0045597016493772 1102 32-33 0.89884377412411 1102 34-35 0.973312462562518 1102 36-37 0.9187556791815013 1102 38-39 0.7069815367967891 1102 40-41 1.0292755316137772 1102 42-43 1.0641213452713814 1102 44-45 0.8714900967101471 1102 46-47 0.847824082647854 1102 48-49 0.8584784445817419 1102 50-51 0.8646101147473289 1102 52-53 0.7813328115588121 1102 54-55 0.8542901469010005 1102 56-57 1.5617763740068682 1102 58-59 -8.239603461914413 1102 60-61 -9.170408934707002 1102 62-63 -2.203401898423021 1102 64-65 0.6404531071620845 1102 66-67 -0.9570153461440007 1102 68-69 -3.7393410074074502 1102 70-71 -3.8725667893129536 1102 72-73 -6.54615793953916 1102 74-75 -7.29469218978322 1102 76-77 -8.321495429692607 1102 78-79 -11.466483913637472 1102 80-81 -7.352425629257329 1102 82-83 -3.0822365603492266 1102 84-85 -2.1727086693644893 1102 86-87 -1.97002728052858 1102 88-89 -2.122154301554577 1102 90-91 -7.769534576307365 1102 92-93 -13.842031850500346 1102 94-95 -12.211768526593268 1102 96-97 -12.255123207473748 1102 98-99 -11.705441795430412 1102 100-101 -11.482458043982636 1103 1 0.2909507965933926 1103 2 0.2359487607095403 1103 3 0.2644203423429339 1103 4 0.1654989520932446 1103 5 0.15125085033294283 1103 6 0.18322845747152172 1103 7 0.28113356788430366 1103 8 0.2526134659062578 1103 9 0.310433697234032 1103 10-11 0.2961588546829361 1103 12-13 0.24564689320220623 1103 14-15 0.44411925463831636 1103 16-17 0.4861919642150099 1103 18-19 0.38387074192184656 1103 20-21 0.2512856252597331 1103 22-23 0.3669534259005971 1103 24-25 0.34320192999662424 1103 26-27 0.20231790277887995 1103 28-29 0.4304722196861306 1103 30-31 0.35795726682359685 1103 32-33 0.28750200850168994 1103 34-35 0.35254625511325344 1103 36-37 0.1535106232776755 1103 38-39 0.20210710989577763 1103 40-41 0.26722349697107717 1103 42-43 0.21399806295349322 1103 44-45 0.22582744733069404 1103 46-47 0.31155863025665553 1103 48-49 0.178612334161933 1103 50-51 0.1720114831256936 1103 52-53 0.35123548911972335 1103 54-55 0.04571143700062663 1103 56-57 0.6241829721946388 1103 58-59 -6.6136099826816235 1103 60-61 -8.623513900465245 1103 62-63 -2.751580219928723 1103 64-65 -0.5560655745845189 1103 66-67 -1.908395189946411 1103 68-69 -4.200052035407577 1103 70-71 -5.0101921536348115 1103 72-73 -7.711344768539849 1103 74-75 -8.293061248228359 1103 76-77 -9.09218310925824 1103 78-79 -12.098620169666054 1103 80-81 -8.598861826736037 1103 82-83 -4.822832015790187 1103 84-85 -3.9203474692443443 1103 86-87 -3.7196643428290592 1103 88-89 -3.8288381802229985 1103 90-91 -9.068393499718685 1103 92-93 -14.599611440725056 1103 94-95 -12.207280279707508 1103 96-97 -12.233714208695037 1103 98-99 -11.8513391748968 1103 100-101 -11.512276089524194 1104 1 0.2362257645339021 1104 2 0.1974415624238901 1104 3 0.17106563863796254 1104 4 0.19425500837629528 1104 5 0.09087591068426804 1104 6 0.19736591645524726 1104 7 0.1140219638630029 1104 8 0.1851646814125445 1104 9 0.18975041590023523 1104 10-11 0.3104256730788464 1104 12-13 0.2271543012173325 1104 14-15 0.3321222364957279 1104 16-17 0.4128169938742161 1104 18-19 0.3792519223013997 1104 20-21 0.3675860818246761 1104 22-23 0.5064497957705427 1104 24-25 0.43566656224798095 1104 26-27 0.35692935432330586 1104 28-29 0.38973094008446907 1104 30-31 0.5035527608270272 1104 32-33 0.39719692096816317 1104 34-35 0.25876840349076247 1104 36-37 0.21929618019601804 1104 38-39 0.26166821652767425 1104 40-41 0.39173744004664 1104 42-43 0.3744132761712464 1104 44-45 0.11540084261488914 1104 46-47 0.14659454003599137 1104 48-49 0.23463223918162868 1104 50-51 0.10185874261457428 1104 52-53 0.13339320608250915 1104 54-55 0.022150612106649703 1104 56-57 0.6902314287548705 1104 58-59 -6.774783891311188 1104 60-61 -8.738773825332512 1104 62-63 -2.483576980336206 1104 64-65 -0.22396199183049958 1104 66-67 -1.8073958860279333 1104 68-69 -4.279586825514798 1104 70-71 -4.8435702954945725 1104 72-73 -7.87977333007499 1104 74-75 -8.669651827951334 1104 76-77 -9.118159217017485 1104 78-79 -12.046342588173559 1104 80-81 -8.400376728743577 1104 82-83 -4.709711685763342 1104 84-85 -3.855130700250939 1104 86-87 -3.662044266799171 1104 88-89 -3.985763689662992 1104 90-91 -9.613215306390602 1104 92-93 -15.070263331497312 1104 94-95 -12.535519016388406 1104 96-97 -12.196920456980902 1104 98-99 -11.603530218385565 1104 100-101 -11.210910024619805 1105 1 0.1495235159826045 1105 2 0.17272052073525046 1105 3 0.12234994859153048 1105 4 0.20229891085638485 1105 5 0.11389995494722172 1105 6 0.07898469909528671 1105 7 0.16963606332095793 1105 8 0.08945951292840704 1105 9 0.13889713893541966 1105 10-11 0.18306342166555822 1105 12-13 0.27154485622244096 1105 14-15 0.3181106191539911 1105 16-17 0.27130621458341153 1105 18-19 0.22445433450163677 1105 20-21 0.17319862154234755 1105 22-23 0.29980252930203477 1105 24-25 0.2730637865044727 1105 26-27 0.2468200586178355 1105 28-29 0.2914771559812408 1105 30-31 0.2885021088803441 1105 32-33 0.26333872265139746 1105 34-35 0.32495075842130206 1105 36-37 0.26388578777031313 1105 38-39 0.33425397377168053 1105 40-41 0.31962812156401554 1105 42-43 0.3446021830012711 1105 44-45 0.3695048659030462 1105 46-47 0.2292121011270538 1105 48-49 0.3787951711368507 1105 50-51 0.2943606502016962 1105 52-53 0.37596653987138495 1105 54-55 0.3054178669191927 1105 56-57 0.9110872359650273 1105 58-59 -6.385086050140281 1105 60-61 -8.308648011234741 1105 62-63 -2.3769759118893568 1105 64-65 -0.13857235137787072 1105 66-67 -1.6199554594414423 1105 68-69 -4.366187171034408 1105 70-71 -4.843046866462506 1105 72-73 -6.186605834270196 1105 74-75 -6.716025481313665 1105 76-77 -8.535625386745945 1105 78-79 -11.908874655691001 1105 80-81 -8.045507541437587 1105 82-83 -4.09813356035329 1105 84-85 -3.1637621544190857 1105 86-87 -2.8598777132755693 1105 88-89 -2.8194302627452394 1105 90-91 -8.144142282321582 1105 92-93 -11.277504388615 1105 94-95 -11.757927334607114 1105 96-97 -11.976556883997187 1105 98-99 -11.31208531955329 1105 100-101 -11.01427683542628 1106 1 0.09113744076049102 1106 2 0.11841407082740574 1106 3 0.12827389302601944 1106 4 0.09217415349971247 1106 5 0.08478336817858434 1106 6 0.039920923159066035 1106 7 0.1318727177462975 1106 8 0.18851008232631727 1106 9 0.12899468653334623 1106 10-11 0.2225759441296944 1106 12-13 0.23352902369727957 1106 14-15 0.25634615209603595 1106 16-17 0.1582639153588019 1106 18-19 0.18561929826025647 1106 20-21 0.12048967250395748 1106 22-23 0.1848849629207976 1106 24-25 0.1065835740967529 1106 26-27 0.14667803470818086 1106 28-29 0.184109071986434 1106 30-31 0.2656851328099208 1106 32-33 0.28520243791719935 1106 34-35 0.2853201125112719 1106 36-37 0.16701812739589883 1106 38-39 0.19575262477435018 1106 40-41 0.24408519035598175 1106 42-43 0.2798863150850437 1106 44-45 0.297494607088602 1106 46-47 0.20116934993853874 1106 48-49 0.23132287499171156 1106 50-51 0.21305311098424795 1106 52-53 0.2429993738995222 1106 54-55 -0.04273482640262216 1106 56-57 0.871856616406788 1106 58-59 -6.376285623608929 1106 60-61 -8.882003896800544 1106 62-63 -2.4854794211209104 1106 64-65 -0.2081741788440965 1106 66-67 -1.536735883123125 1106 68-69 -4.125200480222144 1106 70-71 -4.538895999381259 1106 72-73 -6.06466245079185 1106 74-75 -6.662459792574307 1106 76-77 -8.380921361815924 1106 78-79 -11.659556355136543 1106 80-81 -7.8413977341274155 1106 82-83 -3.992850485405416 1106 84-85 -3.0523018820457217 1106 86-87 -2.7984008178232465 1106 88-89 -2.9997163635531052 1106 90-91 -7.988579985609579 1106 92-93 -11.244464755211345 1106 94-95 -11.376580114491503 1106 96-97 -11.794481312569273 1106 98-99 -11.539040140016379 1106 100-101 -11.229237606492012 1107 1 0.04904504708308011 1107 2 0.09377370802720719 1107 3 -0.006385415317129883 1107 4 0.01895585254541743 1107 5 0.03361498531693741 1107 6 -0.028186777267464436 1107 7 0.04944200833871548 1107 8 0.03140417502711301 1107 9 -0.09470126950252933 1107 10-11 0.13418401694533344 1107 12-13 0.15006654847969259 1107 14-15 0.0800309410229687 1107 16-17 0.10341697930008564 1107 18-19 0.041506388139048056 1107 20-21 0.106162961438649 1107 22-23 0.10907626797243353 1107 24-25 0.15555215820978674 1107 26-27 0.1522777160652211 1107 28-29 0.1758038263156152 1107 30-31 0.121615379862682 1107 32-33 0.09793463593793916 1107 34-35 0.014869013037888124 1107 36-37 0.0479473830000714 1107 38-39 0.040353479742812226 1107 40-41 0.01253944643939775 1107 42-43 0.07059121322650697 1107 44-45 0.06303080936480399 1107 46-47 0.048917128662630205 1107 48-49 0.0597747820266008 1107 50-51 0.0727450813460706 1107 52-53 0.15127120952779194 1107 54-55 0.1695076923697343 1107 56-57 0.8735616037025551 1107 58-59 -6.657539912067826 1107 60-61 -8.851964728749149 1107 62-63 -2.644454475260087 1107 64-65 -0.381734024963162 1107 66-67 -1.822935192588055 1107 68-69 -4.203169315975474 1107 70-71 -4.789541150954225 1107 72-73 -6.240064869885842 1107 74-75 -6.886579916181322 1107 76-77 -9.181784576294096 1107 78-79 -12.3630416696783 1107 80-81 -8.261497627517532 1107 82-83 -4.356067752818934 1107 84-85 -3.4231589835206613 1107 86-87 -3.1730397758599693 1107 88-89 -3.304998395938533 1107 90-91 -8.298721079647489 1107 92-93 -11.693934043683937 1107 94-95 -11.806994014610893 1107 96-97 -12.296102977612602 1107 98-99 -11.710481531813432 1107 100-101 -11.218503032848883 1108 1 -0.0389665429205408 1108 2 -0.014615327871304373 1108 3 -0.018421867835556327 1108 4 -0.04140036882528619 1108 5 -0.20310942136179477 1108 6 -6.06933148894484E-4 1108 7 -0.06859892458901129 1108 8 -0.10450490665983381 1108 9 -0.052786231863066746 1108 10-11 0.03031815197753218 1108 12-13 -0.05436685700316701 1108 14-15 0.07416269596513558 1108 16-17 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1.0068117562864387 2311 58-59 4.282440541743675 2311 60-61 3.9774175189852734 2311 62-63 1.4579293099018038 2311 64-65 1.3886484777215422 2311 66-67 -0.2671918579928487 2311 68-69 -3.093507859050483 2311 70-71 -0.6991825208870868 2311 72-73 -0.6814185786399101 2311 74-75 1.5456902229597276 2311 76-77 4.447347742903663 2311 78-79 5.1234478966744454 2311 80-81 2.986536164983839 2311 82-83 1.5752656371700162 2311 84-85 1.2393950002706795 2311 86-87 1.16311796883436 2311 88-89 1.3383280872154337 2311 90-91 2.783569919062309 2311 92-93 4.654418822961613 2311 94-95 8.467755477419981 2311 96-97 9.249023222778321 2311 98-99 9.747007439655315 2311 100-101 9.060881918903705 2312 1 -0.26174290446117254 2312 2 -0.25925198118417825 2312 3 -0.3341224968459713 2312 4 -0.1580411103195516 2312 5 -0.16995291634483323 2312 6 -0.14902535660163352 2312 7 -0.20873576387882053 2312 8 -0.25577810364931963 2312 9 -0.3315086310280648 2312 10-11 -0.281843258709948 2312 12-13 -0.4311232445106725 2312 14-15 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5.813880762265654 2312 80-81 3.3113556823208192 2312 82-83 1.9093591496493723 2312 84-85 1.5377355738588285 2312 86-87 1.4058162676660864 2312 88-89 1.5672143472518698 2312 90-91 2.932844828312149 2312 92-93 4.8749814382516305 2312 94-95 8.92322933858848 2312 96-97 9.495818669094861 2312 98-99 9.927326473872215 2312 100-101 9.086526739592243 2313 1 -0.2611089993478153 2313 2 -0.3001316982261244 2313 3 -0.3397026525359337 2313 4 -0.3421880387806766 2313 5 -0.4683134300013734 2313 6 -0.4205387352227703 2313 7 -0.5453132349627197 2313 8 -0.3888290567274524 2313 9 -0.3868138079914516 2313 10-11 -0.4204265559906659 2313 12-13 -0.4076768134322819 2313 14-15 -0.4940233445249973 2313 16-17 -0.538701521996046 2313 18-19 -0.5209550095353919 2313 20-21 -0.47271226790736165 2313 22-23 -0.5248021724066732 2313 24-25 -0.5161132812462697 2313 26-27 -0.7120515522274573 2313 28-29 -0.6799606868358197 2313 30-31 -0.6488273628245125 2313 32-33 -0.5436382826738111 2313 34-35 -0.7016668813605094 2313 36-37 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3.732885849764891 2314 60-61 3.491411827538375 2314 62-63 1.2318389667320382 2314 64-65 0.9059134332942342 2314 66-67 1.581100887932287 2314 68-69 -0.23177577464355537 2314 70-71 2.352799405799084 2314 72-73 7.927464722399293 2314 74-75 9.367747594616432 2314 76-77 8.338406033435643 2314 78-79 5.851146046594213 2314 80-81 2.9891132906591587 2314 82-83 1.341810027125927 2314 84-85 1.0250332766010466 2314 86-87 1.01963064364341 2314 88-89 1.0442533928247109 2314 90-91 2.168374637815372 2314 92-93 4.184294104316546 2314 94-95 7.932010823951156 2314 96-97 8.686485435044814 2314 98-99 8.983289963655418 2314 100-101 8.21746353185832 2315 1 -0.7471931974310237 2315 2 -0.5705671655651408 2315 3 -0.5530556500445556 2315 4 -0.36573716679857426 2315 5 -0.7979022779076388 2315 6 -0.7076456699307272 2315 7 -0.7719412341547027 2315 8 -0.737092761173777 2315 9 -1.208806760311127 2315 10-11 -1.0112251995740635 2315 12-13 -0.8348869757084785 2315 14-15 -1.0166993681968322 2315 16-17 -1.0071626849381161 2315 18-19 -0.9475414857700528 2315 20-21 -1.0166380390747136 2315 22-23 -0.9133031188308394 2315 24-25 -0.8857796386728936 2315 26-27 -0.9388148751114684 2315 28-29 -0.9200280961773686 2315 30-31 -1.0461071415183767 2315 32-33 -0.9942480342094129 2315 34-35 -1.03996152228045 2315 36-37 -1.1417705945648393 2315 38-39 -1.1022224052636886 2315 40-41 -0.9598224641479121 2315 42-43 -1.047131854286306 2315 44-45 -1.0360112755477573 2315 46-47 -1.1285396450094467 2315 48-49 -0.9932638950238513 2315 50-51 -0.9756552987616232 2315 52-53 -0.9281581920210442 2315 54-55 -1.1387580116487044 2315 56-57 0.16017154548490709 2315 58-59 3.5865935406863123 2315 60-61 3.359258824820916 2315 62-63 0.78859632591519 2315 64-65 0.6686679280039733 2315 66-67 1.5707913920132128 2315 68-69 5.814335844969552 2315 70-71 4.854302305841292 2315 72-73 8.657383056451906 2315 74-75 9.484804964707347 2315 76-77 8.134104580806163 2315 78-79 5.691199589483059 2315 80-81 2.870103193864619 2315 82-83 1.2492816076607873 2315 84-85 0.7471188883131354 2315 86-87 0.6684509453616165 2315 88-89 0.7927651945500358 2315 90-91 2.0965275741377525 2315 92-93 4.066877726547514 2315 94-95 7.8829790064807135 2315 96-97 8.632998744886935 2315 98-99 8.94141245309149 2315 100-101 8.202304586781276 2316 1 -0.49473597688514914 2316 2 -0.4397097181010956 2316 3 -0.5901054204366254 2316 4 -0.45203355909313103 2316 5 -0.6173746931678892 2316 6 -0.5563836821471426 2316 7 -0.6633476866510506 2316 8 -0.6227465446918856 2316 9 -0.6900478657547211 2316 10-11 -0.6690123072966188 2316 12-13 -0.603841489374922 2316 14-15 -0.7914231784658554 2316 16-17 -0.8991780019144358 2316 18-19 -1.0022038227337475 2316 20-21 -0.7343493932969096 2316 22-23 -0.7822793951232754 2316 24-25 -2.236706384206663 2316 26-27 -1.5297777612758168 2316 28-29 -1.2242603156228924 2316 30-31 -0.757779244766148 2316 32-33 -0.7224030036683757 2316 34-35 -0.8027753075591306 2316 36-37 -0.7547247365549694 2316 38-39 -1.1776602252843276 2316 40-41 -0.8389667772558553 2316 42-43 -1.1512237170203719 2316 44-45 -1.0843130571488047 2316 46-47 -0.9347133841220199 2316 48-49 -1.1754711143471113 2316 50-51 -1.1436882018959267 2316 52-53 -0.9941855102297552 2316 54-55 -0.9173418760064109 2316 56-57 0.13061936653254236 2316 58-59 3.5590413226101276 2316 60-61 3.420559740807402 2316 62-63 0.9762209719668604 2316 64-65 0.6042684053146026 2316 66-67 5.656211166170948 2316 68-69 8.658849217980961 2316 70-71 5.404212076526788 2316 72-73 8.878733659692337 2316 74-75 9.734338657653808 2316 76-77 8.217264709719156 2316 78-79 5.783390719134516 2316 80-81 2.7982926411917255 2316 82-83 1.4081084309531562 2316 84-85 1.0436395579173485 2316 86-87 1.0130951386528615 2316 88-89 1.0413171240880352 2316 90-91 2.349215136185684 2316 92-93 4.098814013403377 2316 94-95 8.189750361974433 2316 96-97 8.967032802173991 2316 98-99 9.288814142262504 2316 100-101 8.207578967035483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 34.0 4 90.0 5 184.0 6 461.0 7 1033.0 8 2144.0 9 3652.0 10 6316.0 11 9790.0 12 13459.0 13 18274.0 14 23255.0 15 31798.0 16 42289.0 17 53556.0 18 56053.0 19 54929.0 20 56381.0 21 55940.0 22 54252.0 23 52790.0 24 54054.0 25 57653.0 26 75056.0 27 108238.0 28 150450.0 29 196214.0 30 231015.0 31 244027.0 32 249130.0 33 256327.0 34 260017.0 35 250076.0 36 208911.0 37 129288.0 38 41676.0 39 1867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15237362086851 20.364951011570735 13.976029701243368 22.50664566631739 2 15.717718853742593 22.169506238118824 38.70806902038637 23.404705887752215 3 19.61690804960553 26.373499572371774 30.157542237506913 23.852050140515782 4 12.468879919677004 17.237156400714202 37.76269117440258 32.53127250520621 5 12.468642166445806 39.35873886403296 34.08101211015915 14.091606859362086 6 30.453981495898446 37.554737885877714 17.53916756127742 14.452113056946414 7 27.5950008571851 34.08692414124968 21.04817183561845 17.269903165946772 8 26.580919579490057 34.46857861386178 20.008083451640406 18.942418355007757 9 26.91030172587581 16.47116399835709 20.886306443507895 35.7322278322592 10-11 24.811493032871656 27.208349736763864 27.430250865134138 20.549906365230346 12-13 26.040775131339437 24.872708832743356 28.01484126669339 21.07167476922381 14-15 22.385408222502743 26.07426154915778 27.147039820997108 24.39329040734237 16-17 21.116402458072503 29.493510141827255 27.652463399179787 21.737624000920448 18-19 21.013469925078212 27.824531050452233 30.576175946880795 20.58582307758876 20-21 21.947626154536543 26.638329842858138 30.40456841959653 21.00947558300879 22-23 21.292805578291812 26.933444084488624 30.459048023016482 21.314702314203082 24-25 21.328551671871512 27.1281218009213 30.61671763339554 20.92660889381165 26-27 21.113254898258617 27.163684376839758 30.87617301870143 20.8468877062002 28-29 20.647409909633048 27.857655048527057 30.71771534779434 20.777219694045552 30-31 20.947735237327198 27.239241680681086 30.53998944411116 21.273033637880562 32-33 20.64104522476017 27.4206945743793 30.483362037372725 21.454898163487808 34-35 21.549609976754148 27.22444697468721 30.451446191084102 20.774496857474542 36-37 21.416355423178025 26.888748773305554 30.53209275301874 21.16280305049768 38-39 20.856023945679585 27.47184090772723 30.5886449503476 21.083490196245585 40-41 21.008148629533764 27.346059036578335 30.56488574147217 21.08090659241573 42-43 20.83719284692915 27.41336136556121 30.042136871549594 21.70730891596004 44-45 20.973322681270464 27.294227933925512 30.11055969070764 21.621889694096385 46-47 21.39899040408429 27.74400070825826 29.506727633712437 21.350281253945013 48-49 21.194137077824944 27.75065890819617 29.435949008145606 21.619255005833278 50-51 20.705663059168383 27.848444087055384 29.73616326450315 21.70972958927308 52-53 21.34846380529337 28.086782837006112 29.001437375164095 21.563315982536416 54-55 20.649192259460524 27.69649282418222 29.470161548107892 22.184153368249362 56-57 21.173759394917266 27.716692463877067 29.18669028082575 21.922857860379917 58-59 20.17017052551526 27.221075889707492 30.610105187588964 21.998648397188283 60-61 21.185059664450854 27.723696278034417 29.542619942486592 21.548624115028137 62-63 20.7024658206006 27.705964726414017 29.586077541050027 22.005491911935355 64-65 19.810458945190227 27.860381740929814 29.910381347030523 22.418777966849433 66-67 20.68487859875283 27.461254938796646 29.405712401577222 22.448154060873296 68-69 21.778153778810378 26.676229439197265 29.39754533057225 22.14807145142011 70-71 20.900877302580124 27.18351739012615 29.675745402376428 22.239859904917296 72-73 20.770504836248236 27.0640787660252 29.86042154785648 22.304994849870084 74-75 20.882359157525528 27.62239793050985 29.062463647206783 22.43277926475784 76-77 19.30576035900399 27.810928365401427 29.84754227852934 23.035768997065244 78-79 20.055853702731184 28.098520283379614 28.905747658375724 22.93987835551348 80-81 20.521258324590736 28.18574115798288 28.341624666405853 22.951375851020536 82-83 20.748384948122972 28.186637977943253 28.031639544809085 23.033337529124694 84-85 20.424216548264198 28.159813418800546 28.036857326188485 23.379112706746767 86-87 20.526596753380534 28.18080839156117 27.93811751404688 23.354477341011414 88-89 20.483652587135037 28.192438312554096 27.60779845421425 23.71611064609662 90-91 20.371051741985546 28.057775884051384 27.83150879657447 23.7396635773886 92-93 20.45583498330329 28.021382297217873 28.101434570892884 23.421348148585952 94-95 19.417533904955906 27.73450623521866 28.848542813928752 23.99941704589668 96-97 19.060007633714466 27.630594599976767 29.203986126553712 24.105411639755054 98-99 19.18966664934379 27.920976560159655 28.36017772611073 24.52917906438583 100-101 18.809090318233537 27.922016699424347 28.675917797828205 24.592975184513914 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1943.0 1 1380.0 2 895.5 3 1147.0 4 1357.5 5 1504.5 6 2038.0 7 2971.5 8 3941.0 9 4576.0 10 4712.5 11 4870.0 12 5305.5 13 5700.5 14 5982.0 15 6105.0 16 6232.0 17 6516.0 18 6930.5 19 7437.5 20 8097.0 21 9249.5 22 10822.0 23 12416.5 24 14429.5 25 17449.0 26 21292.0 27 25777.0 28 30369.5 29 35223.5 30 40883.5 31 47404.0 32 55345.5 33 63341.5 34 70318.0 35 77031.5 36 83997.5 37 89512.5 38 92983.0 39 95938.0 40 98637.5 41 101568.5 42 104771.0 43 108291.5 44 111707.0 45 114573.0 46 117575.5 47 121240.0 48 123473.0 49 119905.0 50 111955.0 51 103850.0 52 96732.0 53 90747.5 54 84267.5 55 76445.0 56 68362.5 57 60624.0 58 52666.0 59 44571.5 60 36971.5 61 29751.0 62 23146.5 63 17397.0 64 12806.0 65 9573.0 66 7054.5 67 5111.5 68 3678.5 69 2580.5 70 1955.5 71 1617.5 72 1332.0 73 1089.0 74 835.0 75 565.0 76 341.5 77 190.5 78 114.0 79 66.0 80 35.0 81 21.5 82 13.5 83 7.5 84 4.0 85 3.0 86 3.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01884823824697617 2 0.005375845343485377 3 0.004458018089719581 4 0.0 5 0.06565742818903177 6 2.6223635821879886E-4 7 0.0 8 1.9667726866409916E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9667726866409916E-4 16-17 0.0 18-19 0.001147284067207245 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.2945681344144903E-4 28-29 0.0021470601829164156 30-31 0.008293224828669515 32-33 2.9501590299614876E-4 34-35 0.0010325556604865206 36-37 0.026879226717426884 38-39 0.02556804492633289 40-41 0.027698715336860632 42-43 0.02819040850852088 44-45 0.02892794826601125 46-47 0.031075008448927667 48-49 0.030845551635486215 50-51 0.02574833242260831 52-53 0.022257310903820555 54-55 0.016652008746893727 56-57 0.14711459696074616 58-59 0.7351304609492366 60-61 0.29932641313437025 62-63 4.916931716602479E-4 64-65 0.13829689941563905 66-67 1.3802155123950932 68-69 2.649668942987521 70-71 2.1399142421549535 72-73 1.8070543547133544 74-75 1.516758706165144 76-77 1.089051205910283 78-79 0.41195692898934433 80-81 2.4584658583012397E-4 82-83 2.2945681344144903E-4 84-85 1.638977238867493E-5 86-87 0.003097666981459562 88-89 4.916931716602479E-5 90-91 0.22008186363512697 92-93 0.1695685851332308 94-95 1.2600129216965512 96-97 1.2368705630837422 98-99 0.9712579117528763 100-101 0.3918958475856062 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3050683.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.51312286499038 #Duplication Level Percentage of deduplicated Percentage of total 1 70.92130234042948 30.150860401438084 2 14.463354529512662 12.29764736306174 3 5.672369127398892 7.2345037694606225 4 2.8561022477706968 4.856873030978034 5 1.6505330221568832 3.508465658183973 6 0.9931839422463964 2.5334010578553934 7 0.6418361226330844 1.9100520558482548 8 0.44802606746621054 1.5237589802327582 9 0.3416843236107713 1.3073460867635271 >10 1.7178962688418982 13.416066963099091 >50 0.16458645529697868 4.906496773757453 >100 0.1163649067575575 9.180542343021463 >500 0.00881644624340624 2.437150145908535 >1k 0.003557513396391045 2.7516985559489213 >5k 7.733724774763142E-5 0.1796808885840017 >10k+ 3.093489909905257E-4 1.8054559258578746 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18655 0.6115024078214616 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14079 0.46150321092030866 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11397 0.37358847182745636 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10782 0.35342905178938616 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5465 0.17914021220821696 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4410 0.14455779246811287 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4282 0.1403620107366121 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4282 0.1403620107366121 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4027 0.1320032268183879 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3214 0.10535345691440245 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.277954477734986E-5 0.0 0.0 0.0 0.0 3 3.277954477734986E-5 0.0 0.0 0.0 0.0 4 3.277954477734986E-5 0.0 0.0 0.0 0.0 5 3.277954477734986E-5 0.0 0.0 0.0 0.0 6 6.555908955469972E-5 0.0 0.0 0.0 0.0 7 1.3111817910939943E-4 0.0 0.0 0.0 0.0 8 1.3111817910939943E-4 0.0 0.0 0.0 0.0 9 1.3111817910939943E-4 0.0 0.0 0.0 0.0 10-11 1.3111817910939943E-4 0.0 0.0 1.638977238867493E-5 0.0 12-13 1.4750795149807435E-4 0.0 0.0 6.555908955469972E-5 3.277954477734986E-5 14-15 1.8028749627542424E-4 0.0 0.0 9.833863433204958E-5 3.277954477734986E-5 16-17 2.1306704105277408E-4 0.0 0.0 1.3111817910939943E-4 3.277954477734986E-5 18-19 2.786261306074738E-4 0.0 0.0 1.638977238867493E-4 3.277954477734986E-5 20-21 3.277954477734986E-4 0.0 0.0 2.786261306074738E-4 3.277954477734986E-5 22-23 4.097443097168732E-4 0.0 0.0 7.539295298790468E-4 3.277954477734986E-5 24-25 4.916931716602478E-4 0.0 0.0 0.0017700954179768924 3.277954477734986E-5 26-27 5.080829440489228E-4 0.0 0.0 0.0034254624292330603 3.277954477734986E-5 28-29 7.047602127130219E-4 0.0 0.0 0.009899422522759658 3.277954477734986E-5 30-31 7.539295298790468E-4 0.0 0.0 0.031238906172814412 3.277954477734986E-5 32-33 7.539295298790468E-4 0.0 0.0 0.08304697669341587 3.277954477734986E-5 34-35 8.850477089884462E-4 0.0 0.0 0.16430746819646616 3.277954477734986E-5 36-37 9.833863433204957E-4 0.0 0.0 0.2995230904030343 3.277954477734986E-5 38-39 0.0010161658880978456 0.0 0.0 0.5120328791945935 3.277954477734986E-5 40-41 0.0010325556604865206 3.277954477734986E-5 0.0 0.7458821516362073 3.277954477734986E-5 42-43 0.0011145045224298952 3.277954477734986E-5 0.0 0.9738147162455096 3.277954477734986E-5 44-45 0.001180063611984595 3.277954477734986E-5 0.0 1.24419679134148 3.277954477734986E-5 46-47 0.0012784022463166445 3.277954477734986E-5 0.0 1.559060708700314 3.277954477734986E-5 48-49 0.0012784022463166445 6.555908955469972E-5 0.0 1.8606489104243216 3.277954477734986E-5 50-51 0.0012784022463166445 8.194886194337465E-5 0.0 2.1702025415292248 3.277954477734986E-5 52-53 0.0012784022463166445 9.833863433204958E-5 0.0 2.462448572991687 3.277954477734986E-5 54-55 0.0012784022463166445 9.833863433204958E-5 0.0 2.7712318847943234 3.277954477734986E-5 56-57 0.0012784022463166445 1.1472840672072451E-4 0.0 3.0492679835958043 3.277954477734986E-5 58-59 0.0012784022463166445 1.4750795149807435E-4 0.0 3.2357016445169817 3.277954477734986E-5 60-61 0.0012784022463166445 1.638977238867493E-4 0.0 3.3786040699738384 3.277954477734986E-5 62-63 0.0013275715634826694 1.638977238867493E-4 0.0 3.4975610379708413 3.277954477734986E-5 64-65 0.0013439613358713443 1.9667726866409916E-4 0.0 3.6155673991693007 6.555908955469972E-5 66-67 0.0013767408806486942 1.9667726866409916E-4 0.0 3.7150533175685574 6.555908955469972E-5 68-69 0.0013767408806486942 1.9667726866409916E-4 0.0 3.830076740192278 6.555908955469972E-5 70-71 0.001409520425426044 1.9667726866409916E-4 0.0 4.0422915130808414 6.555908955469972E-5 72-73 0.0014259101978147189 1.9667726866409916E-4 0.0 4.305249021284742 6.555908955469972E-5 74-75 0.0014422999702033937 1.9667726866409916E-4 0.0 4.608295912751341 6.555908955469972E-5 76-77 0.0014422999702033937 1.9667726866409916E-4 0.0 5.029463893823121 6.555908955469972E-5 78-79 0.0014422999702033937 1.9667726866409916E-4 0.0 5.651209909387505 6.555908955469972E-5 80-81 0.0015078590597580935 1.9667726866409916E-4 0.0 6.357461591387896 6.555908955469972E-5 82-83 0.0015078590597580935 1.9667726866409916E-4 0.0 6.9966791043186065 6.555908955469972E-5 84-85 0.0015406386045354434 2.1306704105277408E-4 0.0 7.501385755255463 6.555908955469972E-5 86-87 0.0015734181493127933 2.6223635821879886E-4 0.0 7.94769564717147 6.555908955469972E-5 88-89 0.0015734181493127933 2.786261306074738E-4 0.0 8.394021928859864 6.555908955469972E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 17885 0.0 28.190416 1 TACATGG 18230 0.0 27.240288 2 ACATGGG 17980 0.0 26.541485 3 GTATCAA 26010 0.0 24.51597 1 CATGGGG 5355 0.0 22.983084 4 CATGGGA 12100 0.0 22.953938 4 ATGGGCG 545 0.0 21.630709 5 GAGTACT 13955 0.0 21.461155 12-13 GGTATCA 18810 0.0 21.062176 1 AGTACTT 14575 0.0 20.984735 12-13 ATCAACG 30260 0.0 20.959831 3 TCAACGC 30390 0.0 20.869827 4 CAACGCA 30535 0.0 20.755281 5 AACGCAG 30690 0.0 20.729727 6 ATGGGAG 4510 0.0 20.283947 5 AGAGTAC 23285 0.0 20.238932 10-11 TATCAAC 31565 0.0 20.154692 2 GAGTACA 14065 0.0 19.918629 1 GTACTTT 15190 0.0 19.747313 14-15 ATGGGAT 5185 0.0 19.462215 5 >>END_MODULE