FastQCFastQC Report
Fri 27 May 2016
SRR939722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157834
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180090.5702959686924646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119110.37718892126691905No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102440.3243995726184467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87320.2765186517087345No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50080.15858971687555456No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT41930.13278088715239625No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41310.13081751605689215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35640.1128621707157501No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.10820708118286142No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32030.10143028417579898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG185150.029.5757641
TACATGG187450.028.66212
ACATGGG194450.027.050483
CATTCCG4200.025.7651969
CATGGGA133350.024.6975024
GTATCAA278650.023.9737531
GTCGGGA7350.023.6832732
GAGTACT134750.022.76513912-13
CATGGGG55650.021.9814434
AGTACAT135500.021.6656972
ATGGGAT51450.021.5811525
GAGTACA144350.021.5748561
AGTACTT141900.021.38596212-13
GTACTTT147000.020.96672214-15
ATGGGAG50950.020.7771685
ATCAACG323750.020.5194663
TCAACGC324450.020.5190244
CAACGCA324400.020.4786745
TATCAAC325200.020.3983942
AACGCAG332050.020.1488866
Produced by FastQC (version 0.11.5)