##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939722_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3157834 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.198434433222264 31.0 30.0 33.0 26.0 34.0 2 30.23376149601277 31.0 30.0 33.0 26.0 34.0 3 30.193136181319222 31.0 30.0 34.0 26.0 34.0 4 33.84816269632919 35.0 35.0 37.0 28.0 37.0 5 33.75200849696343 35.0 35.0 37.0 28.0 37.0 6 33.75258832478211 35.0 35.0 37.0 28.0 37.0 7 33.748381643873614 35.0 33.0 37.0 28.0 37.0 8 33.5966950764353 35.0 33.0 37.0 28.0 37.0 9 34.886049425017276 37.0 34.0 39.0 28.0 39.0 10-11 34.6979182566278 37.0 34.0 39.0 27.0 39.0 12-13 34.61887990312347 37.0 34.0 39.0 27.0 39.0 14-15 35.1920770692823 38.0 33.5 40.0 27.0 41.0 16-17 35.03517695990353 38.0 33.0 40.0 26.5 41.0 18-19 35.12590908831814 38.0 33.5 40.0 26.5 41.0 20-21 35.26585137154138 38.0 34.0 40.0 27.0 41.0 22-23 35.19226137282707 38.0 34.0 40.0 26.5 41.0 24-25 35.00532738579672 38.0 34.0 40.0 25.0 41.0 26-27 34.87606457464199 38.0 34.0 40.0 25.0 41.0 28-29 34.764372826437366 38.0 33.5 40.0 24.5 41.0 30-31 34.559698356531726 38.0 33.0 40.0 24.0 41.0 32-33 34.244254606163594 38.0 33.0 40.0 24.0 41.0 34-35 33.95438740605111 38.0 33.0 40.0 23.0 41.0 36-37 33.711637945503156 38.0 32.5 40.0 21.0 41.0 38-39 33.43036714406141 37.0 32.0 40.0 19.0 41.0 40-41 33.14521947638793 37.0 31.5 40.0 17.5 41.0 42-43 32.8073298026432 37.0 31.0 40.0 15.5 41.0 44-45 32.36385953156499 37.0 30.5 40.0 14.0 41.0 46-47 31.927520889318437 36.5 30.0 39.0 9.0 41.0 48-49 31.563391869236952 36.0 30.0 39.0 8.5 40.0 50-51 30.80766452574771 35.5 28.5 38.5 7.5 40.0 52-53 30.93819909469592 35.5 29.0 39.0 6.5 40.0 54-55 31.03117880800574 36.0 29.0 39.0 2.0 40.0 56-57 30.816181597892733 35.5 29.0 39.0 2.0 40.0 58-59 30.302601086694235 35.0 28.0 39.0 2.0 40.0 60-61 29.80910301174793 35.0 27.0 38.0 2.0 40.0 62-63 29.400490494433843 34.0 26.5 38.0 2.0 40.0 64-65 28.897537045962515 34.0 26.0 38.0 2.0 40.0 66-67 28.349674808745487 34.0 26.0 37.5 2.0 39.0 68-69 27.807920080662882 33.0 25.0 36.5 2.0 39.0 70-71 27.25059597812931 33.0 23.5 36.0 2.0 39.0 72-73 26.755996356996597 32.0 22.0 36.0 2.0 38.5 74-75 26.15913186063612 32.0 21.0 35.0 2.0 38.0 76-77 25.510548211210597 31.5 19.0 35.0 2.0 37.0 78-79 24.841670746467358 31.0 16.5 34.5 2.0 36.5 80-81 24.138084680828698 30.5 9.5 34.0 2.0 36.0 82-83 23.368817676926653 30.0 4.5 34.0 2.0 35.5 84-85 22.609610574843387 29.0 2.0 34.0 2.0 35.0 86-87 21.82871265557341 29.0 2.0 34.0 2.0 35.0 88-89 20.97069241131738 28.5 2.0 33.5 2.0 35.0 90-91 20.02667144631415 26.5 2.0 33.0 2.0 34.5 92-93 19.053896911617265 25.5 2.0 32.5 2.0 34.0 94-95 17.67649819464861 22.5 2.0 32.0 2.0 34.0 96-97 15.078910259373988 4.5 2.0 31.0 2.0 34.0 98-99 13.711311139217578 2.0 2.0 30.5 2.0 34.0 100-101 12.421072956969873 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.749980852928573 1101 2 0.8197874410867101 1101 3 0.9355913579427657 1101 4 0.9139892720235423 1101 5 0.8548725291580439 1101 6 0.9973542518919274 1101 7 0.8800423314105785 1101 8 0.7375262618168605 1101 9 0.9059731089799783 1101 10-11 0.997305266903517 1101 12-13 0.7700058594335459 1101 14-15 0.9687070256346999 1101 16-17 0.996436497785929 1101 18-19 0.8763207067906436 1101 20-21 0.9975441070380455 1101 22-23 0.921661937629537 1101 24-25 1.03320698538473 1101 26-27 1.201157248744373 1101 28-29 1.1847214644218624 1101 30-31 1.0726700881598958 1101 32-33 1.0988900323852206 1101 34-35 0.91227204608942 1101 36-37 0.9448623866734565 1101 38-39 0.8971650608166897 1101 40-41 0.8328331768932742 1101 42-43 0.7207360318291265 1101 44-45 0.801591807696397 1101 46-47 0.8660008734680353 1101 48-49 0.7256571807163645 1101 50-51 0.7582892614908658 1101 52-53 0.7777926967850952 1101 54-55 0.7118638458122284 1101 56-57 0.4469408433426203 1101 58-59 0.18077702612194457 1101 60-61 0.5536798017609392 1101 62-63 0.34402723763799514 1101 64-65 0.3922049792990201 1101 66-67 0.1277226513614238 1101 68-69 0.10555315468814896 1101 70-71 0.14979879026919463 1101 72-73 -0.28216756343869775 1101 74-75 -0.3263302630833351 1101 76-77 -0.6327750565664694 1101 78-79 -0.3665573853385595 1101 80-81 -0.5561412295067214 1101 82-83 -0.5533924196489757 1101 84-85 -0.44808280483502116 1101 86-87 -0.5207576573317922 1101 88-89 -0.6988574997723447 1101 90-91 -0.935236624276623 1101 92-93 -1.0314749371520122 1101 94-95 -0.4946769949674312 1101 96-97 0.9217835281618747 1101 98-99 0.9790128638766387 1101 100-101 0.46078838223494323 1102 1 0.9755788899298672 1102 2 0.9350413373106043 1102 3 0.9897610875171097 1102 4 1.0230385728592495 1102 5 1.0522855186243802 1102 6 0.9960384678217835 1102 7 0.9287117632497655 1102 8 0.9566422963952093 1102 9 1.0866069768676994 1102 10-11 1.1244789389020369 1102 12-13 0.9312923833242266 1102 14-15 1.1175457558455761 1102 16-17 1.0448164078493747 1102 18-19 1.165114571393012 1102 20-21 1.1096173704207288 1102 22-23 0.9199483177860017 1102 24-25 1.0909667849781712 1102 26-27 1.2570432675119605 1102 28-29 1.4002819251926795 1102 30-31 1.2079991368447338 1102 32-33 1.0884830592224546 1102 34-35 1.1647670667255525 1102 36-37 1.1527605817823812 1102 38-39 1.1607364453878546 1102 40-41 1.1976025861456563 1102 42-43 1.1251088373156577 1102 44-45 1.1208526031626107 1102 46-47 0.9718964402941772 1102 48-49 0.8473695685523488 1102 50-51 0.9423499583964698 1102 52-53 0.8963921095281613 1102 54-55 0.8627856954662398 1102 56-57 0.7273476111707957 1102 58-59 0.5313097667227531 1102 60-61 0.7527190412016047 1102 62-63 0.7134883369994043 1102 64-65 0.4947595469670354 1102 66-67 0.4684860853771511 1102 68-69 0.41891170995395655 1102 70-71 0.5875102335660465 1102 72-73 0.5812364734393931 1102 74-75 0.318950839974093 1102 76-77 0.2998371074548061 1102 78-79 0.35376632464686253 1102 80-81 0.26598593420654737 1102 82-83 0.12045945197583663 1102 84-85 0.11217191272724492 1102 86-87 -0.0909061142423262 1102 88-89 0.018602305981129774 1102 90-91 -0.15051423664597863 1102 92-93 -0.4346881155261748 1102 94-95 -0.10825505291285253 1102 96-97 1.0708480237574491 1102 98-99 1.1685465490165736 1102 100-101 0.7636755919431604 1103 1 0.6235040511084691 1103 2 0.6059343493502993 1103 3 0.7207006453401839 1103 4 0.6600317681639467 1103 5 0.8254153096894541 1103 6 0.7286709822080084 1103 7 0.7190871839335387 1103 8 0.6722536527326 1103 9 0.8663899449270005 1103 10-11 0.717476307774561 1103 12-13 0.6463022761069794 1103 14-15 0.630422106482996 1103 16-17 0.7284274379013098 1103 18-19 0.7301924870782841 1103 20-21 0.7393067218101237 1103 22-23 0.8577960482982618 1103 24-25 0.8799787412960072 1103 26-27 1.0016110555210105 1103 28-29 0.9934589715316307 1103 30-31 1.0450634415360298 1103 32-33 1.1107940301100925 1103 34-35 1.2189680321136294 1103 36-37 1.0148997637759223 1103 38-39 0.9089179250403063 1103 40-41 1.0933511747806577 1103 42-43 0.816506325158123 1103 44-45 0.8959072379393476 1103 46-47 0.7935453603454228 1103 48-49 0.5560214043289413 1103 50-51 0.8149809816501232 1103 52-53 0.8549231916254278 1103 54-55 0.7181155724718522 1103 56-57 0.595479683027353 1103 58-59 0.35350413264668745 1103 60-61 0.7007349329870678 1103 62-63 0.5838043969782056 1103 64-65 0.4508307613742595 1103 66-67 0.4826133639640062 1103 68-69 0.3165966665106872 1103 70-71 0.37324540601632705 1103 72-73 0.13574338155195775 1103 74-75 0.006298119138161695 1103 76-77 0.020325008437186654 1103 78-79 0.03551724698450798 1103 80-81 -0.03202141964241889 1103 82-83 -0.03527711941334033 1103 84-85 -0.21982815301085523 1103 86-87 -0.2867323059057725 1103 88-89 -0.11183832369369284 1103 90-91 -0.4657899679022002 1103 92-93 -0.37262793950839423 1103 94-95 -0.014487265545152894 1103 96-97 1.5079087008278371 1103 98-99 1.5429082079897452 1103 100-101 0.9381636564691167 1104 1 0.5099007839830989 1104 2 0.5525808351481771 1104 3 0.5247713276789092 1104 4 0.8059722561199862 1104 5 0.675034971912261 1104 6 0.8506585239058779 1104 7 0.6728676929233757 1104 8 0.5958085374183852 1104 9 0.9603573597491035 1104 10-11 0.9373930020246206 1104 12-13 0.8133401447756157 1104 14-15 0.9688919658585675 1104 16-17 0.9347022384988364 1104 18-19 0.9842028380876755 1104 20-21 0.7066815871210252 1104 22-23 0.6630914038031648 1104 24-25 0.7735860852855225 1104 26-27 1.070298586968029 1104 28-29 1.0459851307578276 1104 30-31 1.1124063316336361 1104 32-33 1.1137301450228847 1104 34-35 1.174634530932444 1104 36-37 1.0164774945510757 1104 38-39 0.8170626447187317 1104 40-41 0.9328794244756793 1104 42-43 0.8157018096202577 1104 44-45 0.8864005541514146 1104 46-47 0.8055165515487808 1104 48-49 0.7461161992454599 1104 50-51 1.0260182696981808 1104 52-53 1.0660483161419378 1104 54-55 1.0819497771273063 1104 56-57 1.0083029173187121 1104 58-59 0.8643669508631469 1104 60-61 0.9869138255511309 1104 62-63 0.8257449119307516 1104 64-65 0.6479184346898954 1104 66-67 0.6958617248063916 1104 68-69 0.4756853836913457 1104 70-71 0.6998360188214399 1104 72-73 0.8659044685487949 1104 74-75 0.6486901800516769 1104 76-77 0.5803887691056993 1104 78-79 0.6282583258119665 1104 80-81 0.5903898009675785 1104 82-83 0.7336596602395851 1104 84-85 0.39275521338342045 1104 86-87 0.2666292289983687 1104 88-89 0.2004589579534546 1104 90-91 -0.23718538616420304 1104 92-93 -0.0019227319991408365 1104 94-95 0.28027079486912143 1104 96-97 1.8888090520172085 1104 98-99 2.1139021803343656 1104 100-101 1.865076329804312 1105 1 0.829948993111767 1105 2 0.8214620727075754 1105 3 0.7114157307026225 1105 4 0.6751342341900042 1105 5 0.7552548469597227 1105 6 0.8387799786939993 1105 7 0.7208029845368245 1105 8 0.6271996986946036 1105 9 0.8769109823372077 1105 10-11 0.8806176922320716 1105 12-13 0.5958929562077344 1105 14-15 0.7874326329624566 1105 16-17 0.9583291700280654 1105 18-19 1.0459095160299867 1105 20-21 0.8167665283841217 1105 22-23 0.8279024792464256 1105 24-25 0.8340622223421192 1105 26-27 0.9839698731967914 1105 28-29 0.9306483859670607 1105 30-31 1.0933530829826807 1105 32-33 0.9338193434166868 1105 34-35 0.9843289955119658 1105 36-37 0.9550127251840124 1105 38-39 0.910273384193836 1105 40-41 0.8443614524806904 1105 42-43 0.8486571787825241 1105 44-45 0.8418875066211271 1105 46-47 1.1331036213772343 1105 48-49 0.8415731504495447 1105 50-51 0.8811994566322383 1105 52-53 1.0125039671952898 1105 54-55 1.0265721293645953 1105 56-57 0.9015067533386407 1105 58-59 0.8355340950187973 1105 60-61 0.9424980016812832 1105 62-63 0.7720588983309433 1105 64-65 0.7623453616168199 1105 66-67 0.7193674144040365 1105 68-69 0.4466772088498381 1105 70-71 0.5522569742596382 1105 72-73 0.6099779960993352 1105 74-75 0.6153602984459354 1105 76-77 0.6224897781607339 1105 78-79 0.2535630845937398 1105 80-81 0.21322456101498233 1105 82-83 0.23307114068554924 1105 84-85 0.1993146463796336 1105 86-87 0.1015093677578136 1105 88-89 0.24466997931964585 1105 90-91 0.3316849209244914 1105 92-93 0.5156487187619696 1105 94-95 0.8204852909744247 1105 96-97 2.1738006528134086 1105 98-99 2.2423511753063483 1105 100-101 1.7951213173205662 1106 1 0.9952836259902575 1106 2 1.098703788107187 1106 3 1.0288458801503175 1106 4 0.8967554167761023 1106 5 1.0528831770441869 1106 6 1.0078508039051712 1106 7 0.9786977213789285 1106 8 1.0629371905724412 1106 9 1.2958356087179723 1106 10-11 1.200979940445201 1106 12-13 1.096193476025796 1106 14-15 1.4705223015784412 1106 16-17 1.4554295598916198 1106 18-19 1.3540073068032186 1106 20-21 1.2831814081761976 1106 22-23 1.1089778528656566 1106 24-25 1.3324459130503712 1106 26-27 1.5947642201753425 1106 28-29 1.4636487108533487 1106 30-31 1.5291393078039874 1106 32-33 1.5207345480950494 1106 34-35 1.6919800676367132 1106 36-37 1.7120059025719243 1106 38-39 1.539626860477135 1106 40-41 1.511084974248071 1106 42-43 1.6454408045135196 1106 44-45 1.6984745761467934 1106 46-47 1.6422166818060866 1106 48-49 1.4095718509043813 1106 50-51 1.6995311655341254 1106 52-53 1.5526924012433732 1106 54-55 1.4939941566550488 1106 56-57 1.5993137708824996 1106 58-59 1.5297186304314003 1106 60-61 1.715849203760559 1106 62-63 1.6676323226324392 1106 64-65 1.6133443869579516 1106 66-67 1.5787891168081956 1106 68-69 1.5981148306821957 1106 70-71 1.850088358275233 1106 72-73 1.8876014528894594 1106 74-75 1.7330666012399547 1106 76-77 1.7268594987585253 1106 78-79 1.4456764699088787 1106 80-81 1.4524627026654038 1106 82-83 1.5072426806465629 1106 84-85 1.4718942435206337 1106 86-87 1.5745031949183073 1106 88-89 1.6548145537186087 1106 90-91 1.6036634784293646 1106 92-93 1.739617204791859 1106 94-95 1.8487585203441448 1106 96-97 3.3710553636646097 1106 98-99 3.6407186217001115 1106 100-101 3.1190329482497408 1107 1 0.37983230789023636 1107 2 0.514957828043535 1107 3 0.5068543906206777 1107 4 -0.018725923354168117 1107 5 0.43566033766889944 1107 6 0.44912249764099954 1107 7 0.41556668739886504 1107 8 0.2900647225863793 1107 9 0.5650627458143518 1107 10-11 0.4996413525518264 1107 12-13 0.4772882149619946 1107 14-15 0.5678055091957859 1107 16-17 0.5889352732390165 1107 18-19 0.4267255569960682 1107 20-21 0.3985803236379013 1107 22-23 0.36424983010932266 1107 24-25 0.5743894478736067 1107 26-27 0.7198161949342889 1107 28-29 0.6501504556950906 1107 30-31 0.8098865180808303 1107 32-33 0.8748633222679629 1107 34-35 0.8608797612348198 1107 36-37 0.45385605901469006 1107 38-39 0.5852982515361305 1107 40-41 0.6451525687182951 1107 42-43 0.705894723113353 1107 44-45 0.506484397438193 1107 46-47 0.46868677968273076 1107 48-49 0.5406436857095223 1107 50-51 0.8744665758845365 1107 52-53 0.9132767676788625 1107 54-55 0.7720882127964437 1107 56-57 0.7673131056055844 1107 58-59 0.6231824861017508 1107 60-61 0.673852038033516 1107 62-63 0.594510808716695 1107 64-65 0.6482939263885967 1107 66-67 0.5513558070938025 1107 68-69 0.5126887645125144 1107 70-71 0.5135986343268932 1107 72-73 0.2249566006205974 1107 74-75 0.37977742707130346 1107 76-77 0.44948815604486825 1107 78-79 0.21179307499111744 1107 80-81 0.11501649213627019 1107 82-83 0.2640763169184943 1107 84-85 0.026773548314313445 1107 86-87 0.10506228928796091 1107 88-89 0.2326715344849788 1107 90-91 0.24891578933034353 1107 92-93 0.13364401883427135 1107 94-95 0.3479786724356302 1107 96-97 1.702707886149419 1107 98-99 1.7344534508594531 1107 100-101 1.263423107386636 1108 1 0.4666238222108383 1108 2 0.5007116390783537 1108 3 0.5805081702496082 1108 4 0.6147245821136877 1108 5 0.571339940317543 1108 6 0.5434835803672939 1108 7 0.5627170749357688 1108 8 0.5557682121116017 1108 9 0.7060891274244412 1108 10-11 0.6039839303710366 1108 12-13 0.40022797304275315 1108 14-15 0.4136927084649571 1108 16-17 0.6297619319911334 1108 18-19 0.7652327993684267 1108 20-21 0.5186018002951513 1108 22-23 0.4369908375772056 1108 24-25 0.5507990349122664 1108 26-27 0.5866622005103252 1108 28-29 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0.783621886588648 2311 78-79 0.7520679084020934 2311 80-81 0.5904817725713087 2311 82-83 0.7774743337642391 2311 84-85 1.1005046182097793 2311 86-87 1.1450616120550947 2311 88-89 1.3687253787889553 2311 90-91 1.6635255865951812 2311 92-93 1.727055000118579 2311 94-95 2.101267636133148 2311 96-97 3.691149825184656 2311 98-99 3.9276044624084196 2311 100-101 3.5441439200959266 2312 1 -0.10749055601544555 2312 2 -0.5188456064973224 2312 3 -0.7609614605257988 2312 4 -0.39024917061051667 2312 5 -2.0213553865491356 2312 6 -0.4763895799868578 2312 7 -0.15274304699760677 2312 8 -0.0030238862180453907 2312 9 0.03711171996745577 2312 10-11 0.02282619497596272 2312 12-13 0.02271509178061848 2312 14-15 0.2474541471781322 2312 16-17 -0.5864378950774309 2312 18-19 -0.1669896633933874 2312 20-21 0.29222375175426407 2312 22-23 0.18134642008788404 2312 24-25 0.10976754424070378 2312 26-27 0.3755149900993402 2312 28-29 0.4846763766652131 2312 30-31 0.17803262855504443 2312 32-33 -0.4599303325740465 2312 34-35 -0.028329769031422813 2312 36-37 0.4184860490377602 2312 38-39 0.44772681943271664 2312 40-41 0.6068118327750014 2312 42-43 0.48136397075746373 2312 44-45 0.6342715298164947 2312 46-47 0.11354697715052353 2312 48-49 0.3495063786187238 2312 50-51 0.34747555326476487 2312 52-53 0.17450341915674983 2312 54-55 0.1408064121376711 2312 56-57 0.23342573991169147 2312 58-59 0.6105516840935756 2312 60-61 0.6118840318826564 2312 62-63 0.7187176221417388 2312 64-65 0.5793389073521169 2312 66-67 0.7079767295780393 2312 68-69 0.7438182436058476 2312 70-71 0.6963445456342505 2312 72-73 0.8070990348545486 2312 74-75 0.9423926697494522 2312 76-77 0.8608374789909377 2312 78-79 0.9466882558557828 2312 80-81 0.8291453583369979 2312 82-83 1.0109644509123932 2312 84-85 1.1562103042850893 2312 86-87 1.3012985765827167 2312 88-89 1.167137430273904 2312 90-91 1.3511123317386264 2312 92-93 1.2577128059445322 2312 94-95 1.7823978158768519 2312 96-97 3.1015575088496092 2312 98-99 3.473424497678911 2312 100-101 3.100697239173231 2313 1 -0.01806056579989601 2313 2 -0.42730111199816534 2313 3 -0.7883348086717028 2313 4 -0.13214132615998864 2313 5 -0.2228173393042141 2313 6 0.19963189047633279 2313 7 -0.04219968236956362 2313 8 0.34770790917044536 2313 9 0.4862444055662607 2313 10-11 -0.538130060374904 2313 12-13 0.09668179115919173 2313 14-15 0.2702966357192551 2313 16-17 -0.34927188774491214 2313 18-19 0.0019744806710022544 2313 20-21 -1.895267512128882 2313 22-23 -0.046418433494800126 2313 24-25 0.2946084886731697 2313 26-27 0.5034746226873779 2313 28-29 0.1841221064512979 2313 30-31 0.37777265627765644 2313 32-33 0.7178946910134698 2313 34-35 0.43586134298888624 2313 36-37 -0.183371249405468 2313 38-39 0.37809933109542726 2313 40-41 0.3938138377138145 2313 42-43 0.35312387241791043 2313 44-45 0.4694866166534908 2313 46-47 0.387050043527541 2313 48-49 0.2434954206161457 2313 50-51 0.5446426125713515 2313 52-53 0.44895480921854514 2313 54-55 0.4000970904097869 2313 56-57 0.5437281515167491 2313 58-59 0.6280094486086334 2313 60-61 0.3532184409717125 2313 62-63 0.3990793046596046 2313 64-65 0.3479260879742476 2313 66-67 0.509094206325404 2313 68-69 0.39115349136212174 2313 70-71 0.6377691551298277 2313 72-73 0.5803846993235915 2313 74-75 0.7134781067882585 2313 76-77 0.7546889271479067 2313 78-79 0.6412904458546755 2313 80-81 0.8412323220122246 2313 82-83 0.9473050044036349 2313 84-85 0.8393852894336895 2313 86-87 0.8524532728279368 2313 88-89 0.9440553173275603 2313 90-91 1.2608969100171485 2313 92-93 1.202036259215042 2313 94-95 1.6180281412680841 2313 96-97 3.223315590485708 2313 98-99 3.4322600060626716 2313 100-101 3.0337963293066608 2314 1 -0.20922980350622922 2314 2 -0.18422197328405332 2314 3 -1.2202536609453105 2314 4 -0.09086802905832059 2314 5 -0.41803920122703175 2314 6 -0.5422193324912925 2314 7 -0.265143681524286 2314 8 -1.5193936962535872 2314 9 -1.431904899761939 2314 10-11 -0.5712043551371906 2314 12-13 -0.4826401523146444 2314 14-15 -0.5534929901877703 2314 16-17 -0.5493171463271196 2314 18-19 -1.682646679139225 2314 20-21 -1.0925573545627927 2314 22-23 -1.7531814198989935 2314 24-25 -1.54781277292701 2314 26-27 -1.63259791828159 2314 28-29 -2.622217480614829 2314 30-31 -1.4164468201290958 2314 32-33 -1.1904313813526386 2314 34-35 -1.6470402697271211 2314 36-37 -3.1291353586243034 2314 38-39 -3.2478461138040586 2314 40-41 -4.177530838850085 2314 42-43 -2.952248877488337 2314 44-45 -3.0325734580222417 2314 46-47 -3.053765942852845 2314 48-49 -1.4392789360534728 2314 50-51 -1.0149828256909572 2314 52-53 -2.5141069887432757 2314 54-55 -1.7206138543914875 2314 56-57 -1.6196472383804483 2314 58-59 -3.5367672273540585 2314 60-61 -2.132060986593725 2314 62-63 -2.2736103684220126 2314 64-65 -1.5768055228666817 2314 66-67 -2.742894622822238 2314 68-69 -2.4931172632228886 2314 70-71 -1.9308348008385785 2314 72-73 -1.2870717385934611 2314 74-75 -1.59510590043228 2314 76-77 -0.6621733081833234 2314 78-79 -0.6300048052867417 2314 80-81 -0.4213275391129443 2314 82-83 -0.19701378789723023 2314 84-85 -0.20241193202791763 2314 86-87 0.0733356161398575 2314 88-89 0.20087470489132642 2314 90-91 0.6267165609847467 2314 92-93 0.802448739199324 2314 94-95 1.3154184343160082 2314 96-97 2.6851730971972962 2314 98-99 2.8993028106735768 2314 100-101 2.736435818005594 2315 1 -2.4221940304149037 2315 2 -1.919354372668618 2315 3 -1.5814039757963272 2315 4 -1.553825700587538 2315 5 -1.851733273235947 2315 6 -3.1521121713176505 2315 7 -2.2072776117827644 2315 8 -1.283131072327997 2315 9 -1.242393699703328 2315 10-11 -3.6674867801648574 2315 12-13 -1.9357894337293757 2315 14-15 -1.1673134653371022 2315 16-17 -2.0319719014836934 2315 18-19 -1.2778180637510488 2315 20-21 -0.9261026772995464 2315 22-23 -1.1491881333787788 2315 24-25 -3.051964448983295 2315 26-27 -4.055300450586113 2315 28-29 -5.970839501291266 2315 30-31 -8.539951133183173 2315 32-33 -6.906470081228086 2315 34-35 -6.1788843750913465 2315 36-37 -4.126698781920577 2315 38-39 -6.319798826381522 2315 40-41 -3.999381268227438 2315 42-43 -4.000294603188124 2315 44-45 -3.180046455643023 2315 46-47 -2.3213687755479917 2315 48-49 -2.8298337910831854 2315 50-51 -2.830753140619098 2315 52-53 -2.1603763030120255 2315 54-55 -3.6638046467826193 2315 56-57 -3.5325661560487376 2315 58-59 -2.2770328744664106 2315 60-61 -2.525831255248601 2315 62-63 -3.0148995223538897 2315 64-65 -2.065633324616581 2315 66-67 -2.6605650337674085 2315 68-69 -1.8693809090164564 2315 70-71 -2.356923668647422 2315 72-73 -1.6534071657921174 2315 74-75 -2.2118060188147233 2315 76-77 -1.5703881626731793 2315 78-79 -1.422767569960186 2315 80-81 -1.2442148752766542 2315 82-83 -0.9103076154290513 2315 84-85 -0.6633222043075335 2315 86-87 -1.323516837692921 2315 88-89 -1.9318936125741324 2315 90-91 -1.6052589411766007 2315 92-93 -0.719021092558787 2315 94-95 -0.24454207916300774 2315 96-97 1.1563361757577368 2315 98-99 1.0650130667575475 2315 100-101 0.6666039634887451 2316 1 -3.571501434821279 2316 2 -2.424366526853131 2316 3 -2.494497698063565 2316 4 -3.087637484358808 2316 5 -2.622738790849869 2316 6 -5.942709076823689 2316 7 -3.4295757172733694 2316 8 -4.6567622796436545 2316 9 -6.4469756343408235 2316 10-11 -5.759313573389381 2316 12-13 -3.628110111276925 2316 14-15 -4.921658103991266 2316 16-17 -4.586286636985491 2316 18-19 -2.675465360891927 2316 20-21 -3.492607288172721 2316 22-23 -4.326836973908165 2316 24-25 -3.0482931135663875 2316 26-27 -4.14809764933597 2316 28-29 -3.9413115852516043 2316 30-31 -3.582627754705726 2316 32-33 -3.4235303119646687 2316 34-35 -5.781364407502505 2316 36-37 -5.698735475982389 2316 38-39 -5.382862645496388 2316 40-41 -4.992284574937322 2316 42-43 -4.133329869615565 2316 44-45 -3.712585908511798 2316 46-47 -3.669941141176732 2316 48-49 -4.416803733591966 2316 50-51 -5.682603277594829 2316 52-53 -3.29329774453689 2316 54-55 -3.5429679372869245 2316 56-57 -2.8225881493349974 2316 58-59 -3.4453490376025755 2316 60-61 -3.647605573263803 2316 62-63 -2.752375889217337 2316 64-65 -3.1560623866222137 2316 66-67 -3.751881544165986 2316 68-69 -3.1868028714349457 2316 70-71 -2.614849933261379 2316 72-73 -2.5869478919564095 2316 74-75 -3.7795897129151186 2316 76-77 -3.8204190304433716 2316 78-79 -3.80689381914026 2316 80-81 -3.8675364402726053 2316 82-83 -3.0000122494530537 2316 84-85 -2.8410038418427384 2316 86-87 -2.4756804754596367 2316 88-89 -2.599964762784417 2316 90-91 -2.288255622891306 2316 92-93 -2.076350258743144 2316 94-95 -1.9980947665882525 2316 96-97 -0.24838369922779435 2316 98-99 0.3475592719510505 2316 100-101 0.10526295179757916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23673.0 3 7435.0 4 7158.0 5 7840.0 6 9284.0 7 11311.0 8 13860.0 9 16763.0 10 20468.0 11 25082.0 12 29937.0 13 34119.0 14 38057.0 15 42422.0 16 47389.0 17 51445.0 18 46291.0 19 44033.0 20 47791.0 21 52667.0 22 58215.0 23 65485.0 24 73835.0 25 83353.0 26 94360.0 27 106060.0 28 119726.0 29 134990.0 30 152668.0 31 173597.0 32 198347.0 33 227134.0 34 258700.0 35 284287.0 36 282699.0 37 212825.0 38 53935.0 39 593.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.815601892452825 19.137654319813368 12.914259407571713 22.132484380162094 2 15.648119732473706 21.177294504652796 39.540465000672114 23.634120762201384 3 19.867176736745815 26.35753840983063 29.4604788169249 24.314806036498656 4 12.309679923586561 16.47157392150371 38.08684798368223 33.1318981712275 5 12.825305216426194 38.98132234025686 33.76026637069922 14.433106072617727 6 30.44306197882709 36.934812003561795 18.10405953579936 14.518066481811747 7 28.006007765950834 33.972039025108046 21.057403594765024 16.964549614176093 8 26.814790102087642 33.731924721979176 20.29394238554187 19.159342790391314 9 27.558255738832116 16.567074855131736 20.85091294520172 35.02375646083443 10-11 24.90449767545336 26.930077757627142 27.42974547566112 20.735679091258376 12-13 26.356338756690402 24.819461926612632 27.850433827337383 20.973765489359582 14-15 22.937628593747846 25.83474455813694 27.005348992551177 24.22227785556404 16-17 21.47937704298368 29.290820669001445 27.493375563980827 21.73642672403405 18-19 21.29669936175048 27.683999644139455 30.45124353753924 20.56805745657082 20-21 22.25990026959095 26.486014027612647 30.345360362754093 20.908725340042306 22-23 21.597648344493162 26.782002879914913 30.299395649411924 21.32095312618 24-25 21.50663678511961 26.967593712941966 30.58305355748408 20.942715944454346 26-27 21.353712337834114 26.977986238205272 30.82445563058177 20.843845793378843 28-29 20.82802109091163 27.713337910034497 30.680306632630227 20.778334366423646 30-31 21.020175858571736 27.186969028686793 30.491129074618417 21.301726038123057 32-33 20.800089377020583 27.350986987645275 30.433954634814253 21.414969000519886 34-35 21.764980248441294 27.149746957942668 30.337902368600574 20.74737042501547 36-37 21.538656530064394 26.82926558438355 30.442563704202037 21.189514181350024 38-39 20.971592271138103 27.43415663746817 30.479937187251068 21.114313904142655 40-41 21.199714715472147 27.277509318860982 30.482137831894697 21.040638133772173 42-43 20.96061556150761 27.39868344155535 29.91518642678246 21.725514570154584 44-45 21.141090947049864 27.232619423716407 30.068522695237647 21.55776693399608 46-47 21.57879754638608 27.659232136515833 29.470912920443514 21.291057396654573 48-49 21.329262914193475 27.74417237958436 29.29165533922725 21.63490936699491 50-51 20.851740623869055 27.857410429140135 29.655843454962188 21.635005492028622 52-53 21.414938242870836 28.17407257935201 28.92067037261304 21.490318805164108 54-55 20.6893059172243 27.730433414577497 29.41473244115566 22.165528227042543 56-57 21.107358143126735 27.924816781612442 29.05220956320711 21.915615512053712 58-59 21.550871445571808 27.40158320004583 29.031549584458787 22.01599576992358 60-61 21.055307898356347 27.82407875303256 29.542636553060262 21.577976795550832 62-63 20.88406568938813 27.67781886127585 29.499543343354873 21.938572105981148 64-65 20.543108047206676 27.939245605009823 29.2366249102347 22.28102143754881 66-67 20.75174380636052 27.832978462347697 28.77670316262143 22.638574568670354 68-69 20.956546223919602 27.494957426641758 28.961559053431845 22.58693729600679 70-71 21.060455857621474 27.61043665428166 28.83426288285518 22.494844605241678 72-73 21.143088474596606 27.322586306983748 28.97970959478541 22.554615623634234 74-75 20.66138643970039 28.06901487204499 28.648894956728814 22.620703731525804 76-77 20.883681658205983 27.53962160040168 28.697437952126915 22.87925878926542 78-79 21.077218545148643 27.63109693189213 28.590765519102263 22.70091900385696 80-81 21.158061688665224 27.734629341973395 28.414951337770823 22.69235763159056 82-83 21.223593343360662 27.6436338632495 28.35971591634186 22.773056877047978 84-85 20.86902990084472 27.659939825672836 28.3486689310598 23.122361342422646 86-87 21.036818657740316 27.557928552589946 28.285976893006616 23.119275896663126 88-89 21.116694284543584 27.44565795240923 27.939351967492048 23.498295795555137 90-91 21.137723809680995 27.382431281239406 28.081521125043956 23.39832378403564 92-93 21.767622550668918 27.14274015284156 28.115092691461413 22.974544605028104 94-95 21.39288337606981 26.865321996474 28.25056486432781 23.491229763128374 96-97 21.30777515763643 26.691893816265942 28.339893710781734 23.660437315315892 98-99 21.444086950445616 26.8192386118928 27.61808947866576 24.118584958995825 100-101 21.410941685574965 26.693781529048255 27.911917356847283 23.9833594285295 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5768.0 1 3618.5 2 1715.0 3 2033.0 4 1822.0 5 1725.5 6 2730.0 7 4737.5 8 7064.5 9 7767.0 10 6348.0 11 5134.5 12 4904.5 13 4909.0 14 5152.5 15 5415.0 16 5630.5 17 6023.0 18 6345.5 19 6776.5 20 7583.5 21 8717.0 22 10274.5 23 12266.0 24 14299.5 25 17253.0 26 21837.5 27 26934.0 28 31049.0 29 35840.0 30 41765.5 31 48026.5 32 56553.5 33 64052.5 34 70357.5 35 76529.0 36 83402.0 37 90724.0 38 93890.5 39 94282.5 40 95442.5 41 98717.0 42 101995.5 43 105032.5 44 107441.5 45 109798.0 46 112342.0 47 118015.0 48 129526.0 49 128768.5 50 116188.5 51 105851.5 52 98977.0 53 95168.0 54 89792.5 55 82275.0 56 74516.0 57 67280.5 58 60627.5 59 53408.0 60 45413.5 61 37782.0 62 30701.5 63 23409.5 64 17125.5 65 13108.5 66 9964.0 67 7574.0 68 5575.5 69 3906.5 70 2957.0 71 2367.5 72 1948.0 73 1634.0 74 1326.0 75 1078.0 76 797.5 77 524.5 78 338.0 79 234.5 80 159.5 81 114.0 82 73.0 83 46.0 84 37.5 85 29.5 86 16.0 87 10.0 88 9.5 89 8.5 90 7.5 91 5.0 92 5.0 93 4.0 94 1.5 95 0.5 96 1.0 97 1.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12134900061244512 2 0.1140655271936397 3 0.12727078117469126 4 0.14129938432482517 5 0.1372459730308813 6 0.138385994957303 7 0.1450994574128976 8 0.14449777917395276 9 0.15190792169569395 10-11 0.146888658491865 12-13 0.14633448116652112 14-15 0.13457008823136365 16-17 0.13800598764849575 18-19 0.15559715931869755 20-21 0.18482605482112105 22-23 0.20115053546196537 24-25 0.22004006543725857 26-27 0.22197176925702872 28-29 0.2246159867808124 30-31 0.2236818021466613 32-33 0.22597767963737167 34-35 0.19583043313866405 36-37 0.1844460475123138 38-39 0.19774630332056722 40-41 0.18661525590008846 42-43 0.18937030888894096 44-45 0.19331288471781607 46-47 0.21302576386219163 48-49 0.2311236119441364 50-51 0.2474480925849807 52-53 0.26857016549951646 54-55 0.2620308730604585 56-57 0.2308702737382649 58-59 0.2236818021466613 60-61 0.20306640564386855 62-63 0.19388289568102693 64-65 0.1857127385416713 66-67 0.18207100183226857 68-69 0.18751777325850566 70-71 0.19122284451937624 72-73 0.22588267781016988 74-75 0.23837541808720786 76-77 0.2555074142592676 78-79 0.24333134673956897 80-81 0.2487464508900721 82-83 0.24624473610709113 84-85 0.23183612564814998 86-87 0.22208260472209748 88-89 0.22474265588374817 90-91 0.22692769790938977 92-93 0.2337678294679201 94-95 0.23813791351920335 96-97 8.736558033132837 98-99 0.29684904272992185 100-101 0.3085976020272123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3157834.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.214912171144405 #Duplication Level Percentage of deduplicated Percentage of total 1 75.19272166346614 36.25410470913327 2 12.609051750156135 12.158886453903854 3 4.906668164243972 7.097237237759203 4 2.4168064394585267 4.661044408525964 5 1.3037764756929655 3.1430734133170253 6 0.8010968222183741 2.3174887754305074 7 0.518439477895442 1.7497559724947886 8 0.3857170381292705 1.4877850493053386 9 0.27814446937593174 1.20696400456651 >10 1.3638819158241997 12.050528100263982 >50 0.12858645714054218 4.319124476702022 >100 0.08601915788617606 7.635971085165647 >500 0.006464119831234147 2.066903920816468 >1k 0.002289375773282999 2.101122356690372 >5k 1.3466916313429407E-4 0.4460733506532195 >10k+ 2.020037447014411E-4 1.3039366852719 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18009 0.5702959686924646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11911 0.37718892126691905 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10244 0.3243995726184467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8732 0.2765186517087345 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5008 0.15858971687555456 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4193 0.13278088715239625 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4131 0.13081751605689215 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3564 0.1128621707157501 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.10820708118286142 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3203 0.10143028417579898 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.166727573393662E-5 0.0 0.0 0.0 0.0 3 3.166727573393662E-5 0.0 0.0 3.166727573393662E-5 0.0 4 3.166727573393662E-5 0.0 0.0 3.166727573393662E-5 0.0 5 3.166727573393662E-5 0.0 0.0 3.166727573393662E-5 0.0 6 6.333455146787324E-5 0.0 0.0 3.166727573393662E-5 0.0 7 6.333455146787324E-5 0.0 0.0 3.166727573393662E-5 0.0 8 6.333455146787324E-5 0.0 0.0 3.166727573393662E-5 0.0 9 6.333455146787324E-5 0.0 0.0 3.166727573393662E-5 0.0 10-11 6.333455146787324E-5 0.0 0.0 6.333455146787324E-5 0.0 12-13 6.333455146787324E-5 0.0 0.0 6.333455146787324E-5 0.0 14-15 9.500182720180985E-5 0.0 0.0 6.333455146787324E-5 0.0 16-17 9.500182720180985E-5 0.0 0.0 6.333455146787324E-5 0.0 18-19 2.5333820587149295E-4 0.0 0.0 9.500182720180985E-5 0.0 20-21 3.800073088072394E-4 0.0 0.0 3.166727573393662E-4 0.0 22-23 3.800073088072394E-4 0.0 0.0 7.441809797475104E-4 0.0 24-25 4.750091360090492E-4 0.0 0.0 0.0014883619594950209 0.0 26-27 4.750091360090492E-4 0.0 0.0 0.0028500548160542956 0.0 28-29 5.541773253438908E-4 0.0 0.0 0.00888267084336922 0.0 30-31 6.333455146787324E-4 0.0 0.0 0.028500548160542954 0.0 32-33 6.333455146787324E-4 0.0 0.0 0.07805983468415376 0.0 34-35 7.600146176144788E-4 0.0 0.0 0.15635717393631204 0.0 36-37 8.23349169082352E-4 0.0 0.0 0.28498964796756254 0.0 38-39 8.866837205502253E-4 0.0 0.0 0.4860768488780601 0.0 40-41 9.025173584171935E-4 0.0 0.0 0.7116270202930237 0.0 42-43 9.81685547752035E-4 0.0 0.0 0.9330446122247085 0.0 44-45 0.0010766873749538449 0.0 0.0 1.19908139566551 0.0 46-47 0.0013775264944262428 0.0 0.0 1.5128090963616199 0.0 48-49 0.0014883619594950209 0.0 0.0 1.8471521935605228 0.0 50-51 0.0014883619594950209 0.0 0.0 2.216645966824095 0.0 52-53 0.0014883619594950209 0.0 0.0 2.5627851242338897 0.0 54-55 0.0014883619594950209 0.0 0.0 2.9201819981670982 0.0 56-57 0.001583363786696831 0.0 0.0 3.3337091183387093 0.0 58-59 0.001583363786696831 0.0 0.0 3.7764334667370103 0.0 60-61 0.0016150310624307674 0.0 0.0 4.264426185796974 0.0 62-63 0.0016150310624307674 0.0 0.0 4.723823354869192 0.0 64-65 0.0016150310624307674 0.0 0.0 5.178280428926916 0.0 66-67 0.001646698338164704 0.0 0.0 5.68726855179848 0.0 68-69 0.0016783656138986407 0.0 0.0 6.237519134951362 0.0 70-71 0.0017100328896325772 0.0 0.0 6.8395457139292315 1.583363786696831E-5 72-73 0.001741700165366514 0.0 0.0 7.4169193187482305 3.166727573393662E-5 74-75 0.0017892010789674188 0.0 0.0 7.9810718359483115 3.166727573393662E-5 76-77 0.0018208683547013553 0.0 0.0 8.561643835616438 3.166727573393662E-5 78-79 0.0018367019925683238 0.0 0.0 9.214939734007551 3.166727573393662E-5 80-81 0.0020267056469719436 0.0 0.0 9.918491598988421 3.166727573393662E-5 82-83 0.00205837292270588 0.0 0.0 10.599353860905925 3.166727573393662E-5 84-85 0.00205837292270588 0.0 0.0 11.241328708222156 3.166727573393662E-5 86-87 0.00205837292270588 0.0 0.0 11.81157717600102 3.166727573393662E-5 88-89 0.00205837292270588 0.0 0.0 12.375983031406971 3.166727573393662E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18515 0.0 29.575764 1 TACATGG 18745 0.0 28.6621 2 ACATGGG 19445 0.0 27.05048 3 CATTCCG 420 0.0 25.765196 9 CATGGGA 13335 0.0 24.697502 4 GTATCAA 27865 0.0 23.973753 1 GTCGGGA 735 0.0 23.683273 2 GAGTACT 13475 0.0 22.765139 12-13 CATGGGG 5565 0.0 21.981443 4 AGTACAT 13550 0.0 21.665697 2 ATGGGAT 5145 0.0 21.581152 5 GAGTACA 14435 0.0 21.574856 1 AGTACTT 14190 0.0 21.385962 12-13 GTACTTT 14700 0.0 20.966722 14-15 ATGGGAG 5095 0.0 20.777168 5 ATCAACG 32375 0.0 20.519466 3 TCAACGC 32445 0.0 20.519024 4 CAACGCA 32440 0.0 20.478674 5 TATCAAC 32520 0.0 20.398394 2 AACGCAG 33205 0.0 20.148886 6 >>END_MODULE