FastQCFastQC Report
Fri 27 May 2016
SRR939722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157834
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195010.617543544087498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145370.4603471873442366No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116430.368702091370224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113070.3580618867236213No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56310.1783184296577971No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47170.14937453963697903No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45730.14481445193129214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44110.13968435326239442No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.13183086888037815No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.10114527869419355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG186550.027.4224571
TACATGG189850.026.417532
ACATGGG189850.025.1619053
GTATCAA283500.023.6522221
CATTCCG5150.023.0280659
CATGGGA129150.022.332324
CATGGGG56800.022.0485614
GAGTACT151800.021.50014112-13
TAAGGTG12900.021.3286295
AGTACTT157450.021.01484512-13
TCAACGC328950.020.3191594
ATCAACG329200.020.2893223
GAGTACA151600.020.2842371
CAACGCA332150.020.1234025
GTAAGGT12750.020.0913094
AGAGTAC252050.020.00650410-11
AGTACAT144050.019.9941642
GTACTTT161800.019.9514414-15
AACGCAG337700.019.8348226
TATCAAC337200.019.7704492
Produced by FastQC (version 0.11.5)