##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939721_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 134614 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.019106482238104 31.0 31.0 33.0 30.0 34.0 2 31.11687491642771 31.0 31.0 34.0 28.0 34.0 3 31.094180397284088 31.0 31.0 34.0 28.0 34.0 4 34.90293728735496 35.0 35.0 37.0 32.0 37.0 5 34.69994948519471 35.0 35.0 37.0 32.0 37.0 6 34.86995409095636 35.0 35.0 37.0 32.0 37.0 7 34.73493841650943 35.0 35.0 37.0 31.0 37.0 8 34.77160622223543 35.0 35.0 37.0 31.0 37.0 9 36.09046607336533 38.0 35.0 39.0 31.0 39.0 10-11 36.00604691933974 37.0 35.0 39.0 30.0 39.0 12-13 35.96159389068002 37.0 35.0 39.0 30.0 39.0 14-15 36.43877679884707 38.0 35.0 40.0 30.0 41.0 16-17 36.424153505578914 38.0 35.0 40.0 30.5 41.0 18-19 36.67157576477929 38.0 35.0 40.0 31.0 41.0 20-21 36.70023548813645 38.0 35.0 40.0 30.5 41.0 22-23 36.46739195031721 38.0 35.0 40.0 30.0 41.0 24-25 36.102994487943306 38.0 34.5 40.0 30.0 41.0 26-27 35.511354688219654 37.5 34.0 40.0 28.0 41.0 28-29 35.12849332164559 37.0 34.0 40.0 27.0 41.0 30-31 34.439586521461365 36.5 33.0 39.0 26.0 40.5 32-33 33.66438111934866 35.0 33.0 39.0 24.5 40.0 34-35 33.01411814521521 35.0 33.0 39.0 22.0 40.0 36-37 32.45395352637913 35.0 32.5 39.0 19.0 40.5 38-39 31.799508223513158 35.0 31.0 39.0 13.5 40.0 40-41 31.440927392396034 35.0 31.0 39.0 5.5 40.0 42-43 30.740877620455528 35.0 30.5 39.0 2.0 40.0 44-45 29.951602359338555 35.0 29.5 39.0 2.0 40.0 46-47 28.964175345803557 35.0 25.0 38.0 2.0 40.0 48-49 28.098507584649443 35.0 23.0 38.0 2.0 40.0 50-51 27.22069026995706 34.0 19.5 38.0 2.0 40.0 52-53 26.75870266094166 34.0 11.5 38.0 2.0 40.0 54-55 26.046365905477884 33.5 2.0 38.0 2.0 40.0 56-57 24.260838397194945 32.0 2.0 37.5 2.0 40.0 58-59 21.001173726358328 26.5 2.0 35.0 2.0 39.0 60-61 20.332313132363645 24.5 2.0 35.0 2.0 39.0 62-63 21.271494792517863 29.0 2.0 35.0 2.0 39.0 64-65 20.528072860177993 28.0 2.0 35.0 2.0 39.0 66-67 16.317336978323205 15.5 2.0 31.5 2.0 36.5 68-69 11.209866730057795 5.0 2.0 21.0 2.0 32.5 70-71 10.337821474735168 2.0 2.0 17.0 2.0 29.0 72-73 11.517457322418174 2.0 2.0 22.5 2.0 31.5 74-75 11.93728735495565 2.0 2.0 23.5 2.0 32.5 76-77 12.593552676541815 2.0 2.0 26.0 2.0 32.5 78-79 14.34761614690894 2.0 2.0 30.0 2.0 34.0 80-81 15.715178213261623 2.0 2.0 32.0 2.0 35.0 82-83 16.18975366603771 2.0 2.0 33.0 2.0 35.0 84-85 16.122305257996942 2.0 2.0 33.0 2.0 35.0 86-87 16.011510689824238 2.0 2.0 33.0 2.0 35.0 88-89 15.654727591483798 2.0 2.0 33.0 2.0 35.0 90-91 14.570152435853625 2.0 2.0 32.5 2.0 35.0 92-93 13.251942591409511 2.0 2.0 31.0 2.0 34.5 94-95 10.743095071835025 2.0 2.0 25.0 2.0 34.0 96-97 9.9327633084226 2.0 2.0 21.5 2.0 33.5 98-99 9.273767958756148 2.0 2.0 17.0 2.0 33.0 100-101 8.889045715898792 2.0 2.0 2.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5612306749961604 1101 2 0.28904318735745704 1101 3 0.6809117429981981 1101 4 0.15746765934707696 1101 5 0.5714606179338517 1101 6 0.3483231816429537 1101 7 0.26560309283791383 1101 8 0.5361909272976675 1101 9 0.2433012537446686 1101 10-11 0.32586539825573624 1101 12-13 0.24374022160983344 1101 14-15 0.43748184511637334 1101 16-17 0.9414508937864312 1101 18-19 0.9621284893598698 1101 20-21 0.9701174462951627 1101 22-23 1.069082274250711 1101 24-25 1.171575661410067 1101 26-27 0.2621778145836302 1101 28-29 0.3301833335379101 1101 30-31 -0.22442093573229727 1101 32-33 0.4950341284252673 1101 34-35 1.0887038498902157 1101 36-37 1.3193585662458034 1101 38-39 1.444817722760476 1101 40-41 1.2971968650222294 1101 42-43 1.1539062681921486 1101 44-45 1.4616514149177995 1101 46-47 1.8610490012681957 1101 48-49 1.213229067515826 1101 50-51 1.1381725074966766 1101 52-53 0.9994417547261207 1101 54-55 1.1779741781177506 1101 56-57 1.1958988798491355 1101 58-59 -6.90903262877632 1101 60-61 -6.527663302188298 1101 62-63 -1.674729168953089 1101 64-65 0.35241097410834143 1101 66-67 -1.3205015178661519 1101 68-69 -3.304233115653025 1101 70-71 -3.5601107497821562 1101 72-73 -4.998786791509994 1101 74-75 -5.168110737071814 1101 76-77 -6.1903592035427994 1101 78-79 -7.966611547218234 1101 80-81 -6.415928425327463 1101 82-83 -4.439255736716063 1101 84-85 -4.01844844051282 1101 86-87 -3.8740381133477673 1101 88-89 -3.8726852990871894 1101 90-91 -7.142909874618287 1101 92-93 -8.941576383708904 1101 94-95 -7.2274561420694114 1101 96-97 -6.79022517975696 1101 98-99 -6.710850788240481 1101 100-101 -6.363289875442776 1102 1 0.411357459995493 1102 2 0.3807287605591121 1102 3 0.005414612342921288 1102 4 -0.14594886274020524 1102 5 -0.10737296007228991 1102 6 0.5852776725010855 1102 7 0.39886080003130076 1102 8 0.32439324946502524 1102 9 0.795883663994239 1102 10-11 0.06405437463369168 1102 12-13 -0.050834714997670005 1102 14-15 0.10817449158641779 1102 16-17 0.30592440239447427 1102 18-19 0.6326876779358699 1102 20-21 0.6785453123034344 1102 22-23 0.4527737195997119 1102 24-25 0.6908269626245342 1102 26-27 0.5815425550080207 1102 28-29 1.569072963859547 1102 30-31 0.35411903490696517 1102 32-33 0.6425384658068296 1102 34-35 0.6277736483687946 1102 36-37 1.1913057169197714 1102 38-39 0.9269677294333718 1102 40-41 1.5106961310038116 1102 42-43 0.817825926539939 1102 44-45 -0.2505132721234382 1102 46-47 -0.5940424173892183 1102 48-49 0.7111604701582905 1102 50-51 0.7793709593851048 1102 52-53 1.441921402397277 1102 54-55 1.0274670381204203 1102 56-57 1.3746990933817678 1102 58-59 -7.692397102284929 1102 60-61 -7.177036050086336 1102 62-63 -2.001300635655344 1102 64-65 0.1147558293065245 1102 66-67 -0.4320723172789389 1102 68-69 -2.552297976189525 1102 70-71 -3.8666968968527557 1102 72-73 -5.127173285571118 1102 74-75 -5.0874020756544915 1102 76-77 -5.541453558273881 1102 78-79 -8.072176741399469 1102 80-81 -5.8707195637232985 1102 82-83 -3.0366799993611995 1102 84-85 -2.550244783766523 1102 86-87 -2.4854420903930095 1102 88-89 -2.854401567604473 1102 90-91 -6.1584910837468065 1102 92-93 -9.403073781279971 1102 94-95 -7.790548361474189 1102 96-97 -7.566449655934726 1102 98-99 -6.841172421765103 1102 100-101 -6.3928174344978945 1103 1 0.3288317180209326 1103 2 0.07641824168166167 1103 3 0.12539240358055537 1103 4 0.341735369038517 1103 5 0.037236367607903276 1103 6 -0.5686694302649116 1103 7 0.10969261913083272 1103 8 0.16287497771052983 1103 9 0.006665008633852665 1103 10-11 0.13996796235829834 1103 12-13 0.38503748366979806 1103 14-15 0.4861020102619662 1103 16-17 0.5162010024348476 1103 18-19 -0.0958354393667733 1103 20-21 0.3685311794546564 1103 22-23 0.45989061844888823 1103 24-25 0.3748568435048085 1103 26-27 0.6320170149313 1103 28-29 0.2375062366235312 1103 30-31 0.522754205510644 1103 32-33 0.8307577268607318 1103 34-35 0.7479281002161571 1103 36-37 0.24743331635445287 1103 38-39 0.48246439811902064 1103 40-41 0.8932420627623472 1103 42-43 0.3783195667579875 1103 44-45 1.0949086943615214 1103 46-47 1.2682632559400773 1103 48-49 0.9938679201885741 1103 50-51 0.42016946534109323 1103 52-53 0.039261398359322186 1103 54-55 -0.18730178885313364 1103 56-57 0.12815659388651213 1103 58-59 -5.276209942987531 1103 60-61 -6.589538573809332 1103 62-63 -3.0725453358370522 1103 64-65 -1.544970599120692 1103 66-67 -2.322239892485884 1103 68-69 -3.842097087839032 1103 70-71 -3.1780486028895005 1103 72-73 -5.3820440709537145 1103 74-75 -5.918110737071814 1103 76-77 -5.828451337397644 1103 78-79 -7.89962980015174 1103 80-81 -6.036528164571269 1103 82-83 -4.181541699341011 1103 84-85 -3.823967779930463 1103 86-87 -3.6063284219092626 1103 88-89 -3.1969464898738043 1103 90-91 -6.601025911124153 1103 92-93 -8.445270429775832 1103 94-95 -7.738483956063327 1103 96-97 -7.051307317957742 1103 98-99 -6.6299620442161435 1103 100-101 -6.276751413904314 1104 1 0.28671876768013504 1104 2 0.1946428440834822 1104 3 -0.048009089441190866 1104 4 0.42093043557535736 1104 5 0.1801282858162807 1104 6 0.2820283951164555 1104 7 0.022951301376856748 1104 8 -0.08964043936185107 1104 9 0.3474634506981147 1104 10-11 0.4967514742051051 1104 12-13 0.5236694213523734 1104 14-15 0.465694674981215 1104 16-17 0.6260679287574717 1104 18-19 0.6473891630350437 1104 20-21 0.8209005400518734 1104 22-23 0.5502022055959159 1104 24-25 0.9414748246797515 1104 26-27 1.1112445384626213 1104 28-29 1.2398593687234936 1104 30-31 0.9478596907427104 1104 32-33 0.9242338709697933 1104 34-35 1.305985549096384 1104 36-37 1.3548659680909054 1104 38-39 1.899333921001201 1104 40-41 0.9333264509480017 1104 42-43 0.9750819815583789 1104 44-45 0.6601066820278625 1104 46-47 -0.017196010531847605 1104 48-49 -0.12784580912009247 1104 50-51 -0.7937626995404372 1104 52-53 -0.6705324997257165 1104 54-55 -0.29992755970675233 1104 56-57 -0.6108485992326038 1104 58-59 -5.82112230910243 1104 60-61 -6.66958563645133 1104 62-63 -2.128655365048349 1104 64-65 -0.76628673024695 1104 66-67 -0.9525547253323623 1104 68-69 -3.075097736978922 1104 70-71 -3.4844832020456193 1104 72-73 -5.668761045961828 1104 74-75 -5.782034787704725 1104 76-77 -5.941807390627115 1104 78-79 -7.381007599567515 1104 80-81 -5.3595564021564694 1104 82-83 -2.971544945040458 1104 84-85 -2.4150907919395515 1104 86-87 -2.335312517981965 1104 88-89 -2.5781133188254444 1104 90-91 -5.0322576540647574 1104 92-93 -8.278360335650547 1104 94-95 -7.201442411110389 1104 96-97 -6.952475769759106 1104 98-99 -6.461226244151229 1104 100-101 -5.920251900759232 1105 1 0.15944288767319748 1105 2 0.20383306430196413 1105 3 0.1869484274653459 1105 4 -0.22377218277040356 1105 5 0.01747872798725325 1105 6 0.1433080899317858 1105 7 0.058498379580427695 1105 8 0.536074837211757 1105 9 0.3886460412998147 1105 10-11 0.41490649414614467 1105 12-13 0.05956330031890644 1105 14-15 0.11364855047973776 1105 16-17 0.2539073600042272 1105 18-19 0.6086341777740785 1105 20-21 0.31803943375743415 1105 22-23 0.7931361400505708 1105 24-25 0.47671845221572795 1105 26-27 1.1827721958234747 1105 28-29 0.7864173711510958 1105 30-31 -0.06172068033411193 1105 32-33 -0.3359967515375786 1105 34-35 -0.1552594656426436 1105 36-37 -0.26538719646605813 1105 38-39 0.08296492498941177 1105 40-41 -0.15584469522852373 1105 42-43 0.4643311579065674 1105 44-45 1.2010291720573427 1105 46-47 0.42246065671282196 1105 48-49 0.2586202911053306 1105 50-51 0.3688435081932191 1105 52-53 -0.16459353665999643 1105 54-55 -0.19775138491916877 1105 56-57 0.21592441966803833 1105 58-59 -4.944149879763085 1105 60-61 -6.5947633718423475 1105 62-63 -1.3751006572279891 1105 64-65 0.19708243916522505 1105 66-67 -0.5212212763987729 1105 68-69 -2.4649017945478473 1105 70-71 -3.2082997444420123 1105 72-73 -4.005287836320767 1105 74-75 -3.8119463535101694 1105 76-77 -4.831524748005302 1105 78-79 -6.4421745841348805 1105 80-81 -4.3365808516102575 1105 82-83 -2.2801542739809815 1105 84-85 -1.8583021670045774 1105 86-87 -1.709770641789838 1105 88-89 -1.747482141436853 1105 90-91 -4.1614842883633525 1105 92-93 -5.8038935418236015 1105 94-95 -5.942338891082645 1105 96-97 -6.394299941772284 1105 98-99 -6.005313291142732 1105 100-101 -5.650961108319488 1106 1 0.04217709747369369 1106 2 0.21146384484807257 1106 3 4.999582183096152E-4 1106 4 0.05116575967696235 1106 5 0.2585838121097126 1106 6 0.10290172788342744 1106 7 0.03565200649961753 1106 8 0.13362011624090542 1106 9 0.4532318645966811 1106 10-11 0.20338217677922188 1106 12-13 0.6104504934787869 1106 14-15 0.4192245365052969 1106 16-17 0.5614599940025684 1106 18-19 0.14407593561171694 1106 20-21 -0.07046383001014789 1106 22-23 -0.09341346002297257 1106 24-25 -0.26332935508407473 1106 26-27 -0.02243144488628701 1106 28-29 0.2421956188296832 1106 30-31 0.26380565964934277 1106 32-33 -0.5144926603359607 1106 34-35 -0.6135200154265874 1106 36-37 0.02320340756078565 1106 38-39 0.08294194071065775 1106 40-41 0.15890201801961723 1106 42-43 -0.021073825085068165 1106 44-45 -0.0027724276484626387 1106 46-47 0.8545650972754757 1106 48-49 0.8768054524549704 1106 50-51 1.1873136745993946 1106 52-53 1.2440669395742603 1106 54-55 0.8296918439123111 1106 56-57 1.6813100958655163 1106 58-59 -3.4434143828429775 1106 60-61 -6.138088737292382 1106 62-63 -0.4582577777575487 1106 64-65 1.3418604110324672 1106 66-67 -0.42919222427043024 1106 68-69 -1.7961282356621258 1106 70-71 -2.4098718691087395 1106 72-73 -3.6669132845142034 1106 74-75 -3.455023488749667 1106 76-77 -5.04364665661981 1106 78-79 -6.936865215742861 1106 80-81 -4.609450208969825 1106 82-83 -2.172335022349099 1106 84-85 -1.8448333796551246 1106 86-87 -1.6138572465521825 1106 88-89 -1.7973810106103372 1106 90-91 -4.643165818196946 1106 92-93 -5.96574883280457 1106 94-95 -5.676295864512819 1106 96-97 -6.29730628543322 1106 98-99 -5.96929692633626 1106 100-101 -6.009263029805192 1107 1 0.0671260324022036 1107 2 -0.141809183067501 1107 3 -0.1892675741229013 1107 4 -0.29315872237731355 1107 5 0.231495007518717 1107 6 -0.10991803673091027 1107 7 0.17017199482759793 1107 8 -0.09743717390596629 1107 9 0.2993071491020771 1107 10-11 0.24615525332820454 1107 12-13 0.43116345914359755 1107 14-15 0.8376906390245082 1107 16-17 0.31163021090753773 1107 18-19 0.434125541681162 1107 20-21 0.6709280579384895 1107 22-23 0.3734915103106502 1107 24-25 0.3441990954557568 1107 26-27 0.3351942724563912 1107 28-29 0.7205051217963216 1107 30-31 0.5408701475396285 1107 32-33 0.378416589736986 1107 34-35 0.6388143878415704 1107 36-37 0.10417802092524653 1107 38-39 0.19776540146350285 1107 40-41 -0.816902864773887 1107 42-43 -0.2600583596634145 1107 44-45 -0.8414068017365821 1107 46-47 -0.2866419837477707 1107 48-49 0.3096427250938163 1107 50-51 -0.27032663276369817 1107 52-53 -0.2772101735470329 1107 54-55 -0.3745927587527973 1107 56-57 -0.3836884451324387 1107 58-59 -5.965591213890541 1107 60-61 -6.844415942817136 1107 62-63 -2.780599962369937 1107 64-65 -1.18836524795012 1107 66-67 -1.9033435714156468 1107 68-69 -2.2020469206149507 1107 70-71 -2.5204399072373276 1107 72-73 -4.2718155313773485 1107 74-75 -4.11013972257906 1107 76-77 -5.225608528033096 1107 78-79 -7.269055664142822 1107 80-81 -5.207932846845518 1107 82-83 -3.2041169501771307 1107 84-85 -2.4574683373440642 1107 86-87 -2.6779560696238676 1107 88-89 -2.881589745169233 1107 90-91 -5.204565487489816 1107 92-93 -6.132561399675497 1107 94-95 -7.062201904781276 1107 96-97 -6.870426604468333 1107 98-99 -6.75997876662417 1107 100-101 -6.200246397181907 1108 1 -0.3030558979092355 1108 2 -0.21149774304283397 1108 3 -0.2860915025842026 1108 4 -0.22100331072144286 1108 5 -0.4402595407390706 1108 6 -0.0975837783281932 1108 7 -0.46627573875241524 1108 8 0.022667667290455995 1108 9 0.2488908678809878 1108 10-11 -0.3673970130917823 1108 12-13 -0.4662309287688231 1108 14-15 -0.6952264130877381 1108 16-17 -0.09509195992193753 1108 18-19 -0.40059539572246905 1108 20-21 -0.20457302130912325 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12-13 -0.8917628436624412 2311 14-15 -0.2626022996125599 2311 16-17 -0.11991465495740528 2311 18-19 -0.6630067432402029 2311 20-21 -1.3322325957772065 2311 22-23 -0.561522365730049 2311 24-25 -0.02367016140517819 2311 26-27 -0.7253206828319279 2311 28-29 -0.7078020012379085 2311 30-31 -0.37972806399290704 2311 32-33 -0.7367545480983537 2311 34-35 -1.3978029417914826 2311 36-37 -1.8646779766079007 2311 38-39 -1.7054106700751959 2311 40-41 -2.356757937237653 2311 42-43 -2.917165976067981 2311 44-45 -1.6090602090754693 2311 46-47 -1.8610329797391358 2311 48-49 -2.1764812342031306 2311 50-51 -2.175507021292841 2311 52-53 -1.8429671886565018 2311 54-55 -1.8737447784876125 2311 56-57 -1.1273671377010466 2311 58-59 1.5604649069851888 2311 60-61 0.48036049874931663 2311 62-63 -1.4485000548768774 2311 64-65 -1.176108078662427 2311 66-67 -1.8050549497690263 2311 68-69 -2.089573901805206 2311 70-71 -0.8934741348043946 2311 72-73 -1.7631815504954496 2311 74-75 -1.212436978206565 2311 76-77 0.13663322343159656 2311 78-79 0.9172687268531696 2311 80-81 -0.0025674444403378516 2311 82-83 -0.9464080386754343 2311 84-85 -1.0865155158824535 2311 86-87 -0.9602564088159724 2311 88-89 -1.1548706579043557 2311 90-91 -0.1665605537864554 2311 92-93 0.3275064387457185 2311 94-95 2.455528591672625 2311 96-97 2.469150947181374 2311 98-99 2.5352725438907857 2311 100-101 1.5214618976068692 2312 1 -0.18893105355863327 2312 2 -0.02276946628467158 2312 3 0.10224697394657767 2312 4 -0.11559483256649372 2312 5 -0.2773555290450602 2312 6 -0.22231985109090857 2312 7 -0.14745163610502487 2312 8 -0.32019411604558456 2312 9 -0.10079241943426354 2312 10-11 0.16746845169085134 2312 12-13 -0.6992989813918555 2312 14-15 -0.112718698291566 2312 16-17 -0.5066018944138477 2312 18-19 -0.8104811626002757 2312 20-21 -0.9992511654269265 2312 22-23 -0.7948069740349624 2312 24-25 -0.2688111932929971 2312 26-27 -0.9784742749492992 2312 28-29 -0.1185810603034625 2312 30-31 0.4992173098363395 2312 32-33 0.018808225979931592 2312 34-35 0.584466561754617 2312 36-37 0.3987349409385246 2312 38-39 0.20523304168919054 2312 40-41 0.042733156909925185 2312 42-43 -0.10340386248452305 2312 44-45 -0.5330762341959279 2312 46-47 0.5719430367190945 2312 48-49 0.07886498419327381 2312 50-51 0.6569330198527403 2312 52-53 0.8052381061299236 2312 54-55 1.4744226190544794 2312 56-57 2.408960077934271 2312 58-59 4.940980309508063 2312 60-61 4.342468668312414 2312 62-63 2.361174570211098 2312 64-65 2.2821237441950295 2312 66-67 0.9401402210392682 2312 68-69 0.13634781331579404 2312 70-71 0.4685798958382339 2312 72-73 4.986398872870907 2312 74-75 4.253644988119028 2312 76-77 4.683977709382546 2312 78-79 4.298623282643328 2312 80-81 2.8373708371903277 2312 82-83 1.7707973102499075 2312 84-85 1.794108164481358 2312 86-87 1.637897453996004 2312 88-89 1.9637747863054855 2312 90-91 2.7686892973315143 2312 92-93 4.299787311465115 2312 94-95 5.220240843310329 2312 96-97 5.615163615688763 2312 98-99 5.797494405186871 2312 100-101 5.203504017686994 2313 1 -0.4338056446164309 2313 2 -0.6338381685747478 2313 3 -0.5733255451373935 2313 4 -0.35185437455123036 2313 5 -0.524302093930558 2313 6 -0.5278269394224182 2313 7 -0.7785857691475542 2313 8 -0.5501286852931315 2313 9 -0.9631152111463734 2313 10-11 -0.38842031602997906 2313 12-13 -0.7880394394071217 2313 14-15 -0.4475060483460922 2313 16-17 -0.7900261037922576 2313 18-19 -0.8346632781946113 2313 20-21 -0.7978607619372795 2313 22-23 -0.4626368540785819 2313 24-25 -0.5587512151032499 2313 26-27 -0.805437197522906 2313 28-29 -1.3301677560504714 2313 30-31 -0.8939124611560274 2313 32-33 -0.20962688852387856 2313 34-35 -0.7976762953882428 2313 36-37 -0.45110148218034496 2313 38-39 -0.7741290084743859 2313 40-41 -1.5722341277138483 2313 42-43 -1.2988782354398118 2313 44-45 -1.2330217085688808 2313 46-47 -0.6365902239134016 2313 48-49 -0.37151669519603914 2313 50-51 -0.3090429888713615 2313 52-53 0.18340059249851492 2313 54-55 -0.5025948291461759 2313 56-57 -0.29253937680945796 2313 58-59 3.200713133935544 2313 60-61 3.0688863346155735 2313 62-63 1.2411909278992148 2313 64-65 1.1424836133335212 2313 66-67 1.0864597412137513 2313 68-69 -0.44557694131888503 2313 70-71 2.1029037780628794 2313 72-73 5.72088633197669 2313 74-75 6.096174977213899 2313 76-77 5.948563314617008 2313 78-79 4.653666903144961 2313 80-81 2.801383923340815 2313 82-83 1.3796304618311481 2313 84-85 0.8344571284944422 2313 86-87 0.8396422740614327 2313 88-89 1.3051597372324224 2313 90-91 2.067380682282442 2313 92-93 2.8013412918358913 2313 94-95 4.555655238075868 2313 96-97 5.3420992754488505 2313 98-99 5.385880446626347 2313 100-101 5.24742441027151 2314 1 0.13167963346426959 2314 2 -0.03803664185718958 2314 3 -0.6534782168931201 2314 4 -0.008724603558860622 2314 5 -0.2849891168313192 2314 6 -0.665067942693959 2314 7 -0.24668827732639897 2314 8 -0.05301854352650537 2314 9 0.07478009964970056 2314 10-11 -0.35543231166793277 2314 12-13 0.24726590410433147 2314 14-15 -0.2195889272992062 2314 16-17 -0.22415914632224343 2314 18-19 -0.0891071167987505 2314 20-21 0.08090150632880722 2314 22-23 -0.31007414960748036 2314 24-25 -0.15430737649911208 2314 26-27 -0.13114603067448627 2314 28-29 -0.030794800761483998 2314 30-31 -0.496965896270531 2314 32-33 -0.2292833770735001 2314 34-35 0.22950472969355218 2314 36-37 0.6010250172744023 2314 38-39 0.1556147210785035 2314 40-41 -0.010701957593887812 2314 42-43 0.38896254972921795 2314 44-45 -0.26208386778371207 2314 46-47 0.3047674642000118 2314 48-49 0.05214742694136376 2314 50-51 -0.11405934655947547 2314 52-53 0.08386406032839488 2314 54-55 0.18052948928348655 2314 56-57 1.1456012993083178 2314 58-59 2.89136198889738 2314 60-61 3.3768198133505827 2314 62-63 1.7848386923485045 2314 64-65 1.5492993167141123 2314 66-67 2.306552434779725 2314 68-69 0.4684088820180836 2314 70-71 1.8807936362962483 2314 72-73 6.478765285084648 2314 74-75 7.307080102622841 2314 76-77 7.164893739916899 2314 78-79 6.05053167958989 2314 80-81 3.9442410661979608 2314 82-83 2.8509499820056305 2314 84-85 2.355176866771437 2314 86-87 2.5997295150647055 2314 88-89 2.8569045572978524 2314 90-91 2.978612961453651 2314 92-93 4.173833112991835 2314 94-95 5.823294278424832 2314 96-97 6.6380643790475435 2314 98-99 7.091387534957864 2314 100-101 6.562282643641193 2315 1 -1.0308782441480453 2315 2 -0.5838381685747471 2315 3 -0.8848009549734606 2315 4 -0.459231423731552 2315 5 -0.8956135693403908 2315 6 -0.5766559792350705 2315 7 -0.43959279490867686 2315 8 -1.3842036267451263 2315 9 -1.2833728223875909 2315 10-11 -0.6044624705967223 2315 12-13 -1.2081214066202364 2315 14-15 -1.3391922310158861 2315 16-17 -1.7715249328320652 2315 18-19 -0.9794525053608893 2315 20-21 -1.34920736615274 2315 22-23 -1.8317235051324516 2315 24-25 -1.4852734633468074 2315 26-27 -1.0786807572418766 2315 28-29 -2.1298750160036306 2315 30-31 -1.5332567234511103 2315 32-33 -1.8788891836058461 2315 34-35 -2.3663531103765294 2315 36-37 -2.667026540728351 2315 38-39 -2.2405809991067045 2315 40-41 -1.366320778767708 2315 42-43 -1.4610562213882936 2315 44-45 -2.274942083276141 2315 46-47 -1.8485925658337763 2315 48-49 -1.7403691542124342 2315 50-51 -2.2263731996441933 2315 52-53 -2.1738008126537096 2315 54-55 -1.2363770305513277 2315 56-57 -0.548335629736858 2315 58-59 1.8027623142634113 2315 60-61 1.5662516741940316 2315 62-63 -0.36999174189001494 2315 64-65 -0.09861708924259105 2315 66-67 1.15338011592101 2315 68-69 3.607409007158868 2315 70-71 3.2242738014820826 2315 72-73 4.938626378815094 2315 74-75 4.741725328501957 2315 76-77 4.0811745558348065 2315 78-79 2.4690650296086627 2315 80-81 1.2352246727553364 2315 82-83 0.18689041499274595 2315 84-85 -1.026784089304149 2315 86-87 -0.7001703723085893 2315 88-89 0.47775926884834874 2315 90-91 1.204617216240285 2315 92-93 2.6217745471052094 2315 94-95 4.191896455874463 2315 96-97 4.227696465144401 2315 98-99 4.577215341239931 2315 100-101 4.583431436032633 2316 1 -0.01678294768719013 2316 2 -0.2800998508177379 2316 3 -0.4191199376607564 2316 4 -0.31306932782225516 2316 5 -0.4126198509399046 2316 6 -0.5831673933610091 2316 7 -0.45315185726504836 2316 8 -0.27182427941863097 2316 9 -0.687080498195769 2316 10-11 -0.7839476858564112 2316 12-13 -0.8990207478183336 2316 14-15 -0.679815794674532 2316 16-17 -0.7015080797602096 2316 18-19 -0.2993161486885967 2316 20-21 -1.1723267165434308 2316 22-23 -0.8120360530104946 2316 24-25 -1.8506404006840285 2316 26-27 -1.480971242916766 2316 28-29 -1.3850075424323052 2316 30-31 -0.562136760221442 2316 32-33 0.27845722362565084 2316 34-35 0.11250394493218607 2316 36-37 0.04302401848720905 2316 38-39 0.5086146497365576 2316 40-41 0.4500956453168605 2316 42-43 1.2795596817831516 2316 44-45 0.9137740190679686 2316 46-47 0.07679428877017358 2316 48-49 0.32941788424321317 2316 50-51 0.9354162901673568 2316 52-53 1.4375060664638042 2316 54-55 1.3194679211876057 2316 56-57 2.501419234672518 2316 58-59 5.070572947019656 2316 60-61 4.904500486818517 2316 62-63 3.3499025433865306 2316 64-65 3.2771298082600886 2316 66-67 6.139897658436716 2316 68-69 9.176085274969498 2316 70-71 6.333244232001464 2316 72-73 8.901894342657599 2316 74-75 9.91833786105903 2316 76-77 8.344925130371347 2316 78-79 6.8261969431983704 2316 80-81 4.79938125311385 2316 82-83 4.272687871710993 2316 84-85 4.64553857041701 2316 86-87 4.838974717319111 2316 88-89 4.763303929488764 2316 90-91 5.212507518063484 2316 92-93 5.126141024813192 2316 94-95 7.162097160639284 2316 96-97 8.391899008426153 2316 98-99 6.725867095491502 2316 100-101 6.653065264744141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 14.0 5 51.0 6 112.0 7 278.0 8 491.0 9 812.0 10 1493.0 11 2136.0 12 2757.0 13 3334.0 14 3942.0 15 4877.0 16 5768.0 17 6436.0 18 6127.0 19 5617.0 20 5595.0 21 5739.0 22 4849.0 23 4267.0 24 3802.0 25 3053.0 26 3012.0 27 3762.0 28 5034.0 29 6083.0 30 6884.0 31 6855.0 32 6817.0 33 6919.0 34 6547.0 35 5568.0 36 3566.0 37 1678.0 38 332.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.149959504543666 23.280057659585534 16.019853919142836 14.550128916727967 2 13.938889673053065 28.556782978849853 35.26510114479715 22.23922620329993 3 24.630047841202867 32.73408017115859 32.66127834071257 9.97459364692598 4 9.964788209250152 23.968532247760262 33.28257090644361 32.78410863654598 5 7.281391009582147 46.35038395492154 31.30663613859546 15.061588896900854 6 21.216218223958876 39.919324884484524 13.985172418916308 24.87928447264029 7 15.237642444322287 43.070557297160775 19.616087479756935 22.07571277876001 8 24.888941714829066 29.849792740725334 19.82334675442376 25.43791879002184 9 20.1435214762209 21.959083007710937 22.67074747054541 35.22664804552275 10-11 22.393287473814016 29.800020800213943 22.90474987742731 24.90194184854473 12-13 29.06384179951565 29.068670420610044 24.482223245724814 17.385264534149496 14-15 23.185353569120366 30.36036638363308 29.939901792546042 16.514378254700514 16-17 15.266242738496738 35.15681875585006 29.46833167426865 20.108606831384552 18-19 14.266771163367716 33.35178121065123 33.71615774049408 18.66528988548697 20-21 17.428350691607115 28.612922875778153 32.944195997444545 21.01453043517019 22-23 14.51743503647466 32.68939337661759 34.41655399884113 18.376617588066622 24-25 18.517390464587635 33.501716017650466 32.98951074925342 14.991382768508476 26-27 16.96071731023519 30.71745880814774 38.23413612254297 14.087687759074093 28-29 11.897704479607755 35.31312681078671 36.233935071688585 16.555233637916945 30-31 14.998458400971765 31.481680974439175 36.80038335667401 16.719477267915053 32-33 13.564339518920765 34.74192877412454 36.958637288840684 14.735094418114015 34-35 14.460395579905283 33.58009100195004 37.71900826446281 14.240505153681864 36-37 15.002749457539458 31.51138424040662 38.34601551585769 15.139850786196238 38-39 14.06809636333913 31.323658358969787 37.84980754083255 16.758437736858532 40-41 12.782147647515615 32.42686918752624 38.04957252889749 16.741410636060653 42-43 14.005558528030438 31.892054574638845 36.06206527554842 18.040321621782297 44-45 13.384832034718894 30.18507578037625 36.63133097762073 19.79876120728413 46-47 17.428250821158393 30.49507304965593 35.28640221155418 16.790273917631495 48-49 15.307043781652801 29.069486606894042 35.93603115211551 19.68743845933765 50-51 10.80397249127813 30.462454161418684 39.13268016838132 19.60089317892187 52-53 13.490910577019532 32.0319959577796 35.42552914819865 19.051564317002217 54-55 11.868335995839061 28.930787234833005 35.25021361964558 23.95066314968236 56-57 17.153048472044517 28.574404141994226 31.82521498497337 22.44733240098789 58-59 17.04601173031504 29.55148575257045 34.02577375538513 19.376728761729378 60-61 16.377935741535527 32.75028314258465 31.942954220314736 18.92882689556509 62-63 12.391773484828787 34.35205235730443 31.712272543244175 21.543901614622605 64-65 12.207634701021954 35.87624953961882 30.171615221782822 21.744500537576407 66-67 18.153520325295165 29.55731669172706 28.50817194819094 23.780991034786837 68-69 20.938884644766997 26.6951871657754 29.59090909090909 22.775019098548512 70-71 20.30733120900573 26.50218952597617 30.278504665798202 22.911974599219896 72-73 20.42199110720589 24.731663346387208 31.84930728618284 22.997038260224063 74-75 18.15258619062895 28.027097918593018 30.37872926612949 23.44158662464854 76-77 15.986613028633245 26.712341630701385 30.86853798797276 26.432507352692607 78-79 15.515440457804539 27.66188419097075 31.274108228693365 25.548567122531356 80-81 16.113480024365963 27.0376781018319 30.014337290326417 26.834504583475717 82-83 14.233342123932594 26.930062735164007 31.627214209570365 27.209380931333037 84-85 14.356976243184217 28.04054555989719 29.956765269585627 27.645712927332966 86-87 14.489636728326277 28.55397073025778 29.119679072877204 27.836713468538743 88-89 13.952486368431218 28.50632177930973 27.1710223305154 30.37016952174365 90-91 15.846648697749915 25.617991750182416 28.44846842285527 30.086891129212397 92-93 16.433727766614574 27.006400238148398 28.621716156880257 27.938155838356778 94-95 18.096969286410523 24.50614008347352 29.924015761366583 27.472874868749365 96-97 16.09788404586386 25.34290649236292 31.015680563552 27.543528898221215 98-99 16.868415027343943 26.356405838879066 28.354746810499172 28.420432323277822 100-101 14.501650288101143 26.095064053555113 29.129729427340706 30.27355623100304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1847.0 1 1287.5 2 646.5 3 570.5 4 506.0 5 448.0 6 554.0 7 744.0 8 870.5 9 898.5 10 864.5 11 811.0 12 756.0 13 762.0 14 776.5 15 740.5 16 726.0 17 708.5 18 708.0 19 753.0 20 816.0 21 896.0 22 1049.0 23 1174.0 24 1264.0 25 1439.5 26 1614.0 27 1793.0 28 1989.5 29 2256.0 30 2487.5 31 2653.5 32 2892.5 33 3004.5 34 3082.5 35 3123.0 36 3045.5 37 2875.5 38 2659.0 39 2549.5 40 2443.0 41 2369.5 42 2460.5 43 2756.0 44 3392.5 45 4408.0 46 5881.0 47 7855.0 48 9365.0 49 9564.5 50 8368.5 51 6248.0 52 3972.0 53 2321.0 54 1444.0 55 933.0 56 668.0 57 523.5 58 414.0 59 309.5 60 252.5 61 210.5 62 171.0 63 135.0 64 97.0 65 71.0 66 52.0 67 38.5 68 38.5 69 33.0 70 25.0 71 21.5 72 14.5 73 9.0 74 7.5 75 6.5 76 4.5 77 3.0 78 3.0 79 1.5 80 1.0 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023028808296313905 2 0.0037143239187603073 3 0.001485729567504123 4 0.0 5 0.0690864248889417 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.428647837520615E-4 16-17 0.0 18-19 3.7143239187603075E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.002971459135008246 30-31 0.01002867458065283 32-33 0.0 34-35 0.0011142971756280921 36-37 0.03120032091758658 38-39 0.029714591350082458 40-41 0.032314618093214675 42-43 0.03417178005259483 44-45 0.03528607722822292 46-47 0.03565750962009895 48-49 0.036028942011974985 50-51 0.028600294174454366 52-53 0.02562883503944612 54-55 0.021543078728809784 56-57 0.1381728497778834 58-59 0.7640364300889952 60-61 0.30160310220333697 62-63 3.7143239187603075E-4 64-65 0.15934449611481719 66-67 1.437071924168363 68-69 2.7589998068551562 70-71 2.202222651432986 72-73 1.9292198434041037 74-75 1.583416286567519 76-77 1.1139257432362162 78-79 0.43309016892745184 80-81 0.0 82-83 3.7143239187603075E-4 84-85 0.0 86-87 0.002971459135008246 88-89 0.0 90-91 0.22880235339563493 92-93 0.181259007235503 94-95 1.305584857444248 96-97 1.2944418856879671 98-99 1.0426107239960183 100-101 0.4059756043205016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 134614.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.90339786352088 #Duplication Level Percentage of deduplicated Percentage of total 1 84.29372452463222 30.26431129005898 2 7.44656638596346 5.347140713447338 3 2.8118598828908983 3.0286597233571544 4 1.4090335395501852 2.0235636709406153 5 0.9290103660176698 1.6677314395233778 6 0.5979599015124868 1.2881275350260746 7 0.3703627071651735 0.930809574041333 8 0.28139289482940555 0.8082368847222429 9 0.2565641099915168 0.8290370986673006 >10 1.3552378390680928 9.231580667686867 >50 0.09310794314208272 2.4358536259230097 >100 0.09310794314208272 7.104758791804716 >500 0.026897850241046118 7.2355029937450785 >1k 0.035174111853675694 27.804685991055912 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4377 3.2515191584827727 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4022 2.987802160250791 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 3791 2.816200395204065 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3571 2.6527701427786115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3045 2.262023266525027 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2813 2.089678636694549 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2516 1.8690477959201868 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 2238 1.6625313860371134 TruSeq Adapter, Index 11 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1852 1.3757855795088179 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1362 1.0117818354703076 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1200 0.8914377405024737 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1191 0.8847519574487052 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1191 0.8847519574487052 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 1121 0.8327514225860609 RNA PCR Primer, Index 11 (95% over 23bp) TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 1083 0.8045225608034825 TruSeq Adapter, Index 11 (96% over 28bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1044 0.7755508342371521 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1012 0.7517791611570862 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 997 0.7406361894008052 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 938 0.6968071671594337 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 913 0.6782355475656321 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 888 0.6596639279718306 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 783 0.581663125677864 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 734 0.5452627512740131 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 730 0.5422912921390048 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 708 0.5259482668964595 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 651 0.48360497422259197 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 619 0.4598333011425261 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 613 0.4553761124400137 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 598 0.44423314068373276 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 568 0.4219471971711709 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 484 0.3595465553359978 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 482 0.35806082576849363 TruSeq Adapter, Index 11 (96% over 26bp) ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 456 0.33874634139094 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 423 0.314231803527122 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 411 0.3053174261220972 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 381 0.2830314826095354 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 351 0.26074553909697357 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 337 0.2503454321244447 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 307 0.22805948861188288 TruSeq Adapter, Index 11 (95% over 24bp) TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 281 0.20874500423432926 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.2005734916130566 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 256 0.19017338464052774 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 248 0.18423046637051124 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 244 0.181259007235503 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 237 0.17605895374923855 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 230 0.17085890026297415 No Hit CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA 226 0.1678874411279659 RNA PCR Primer, Index 11 (96% over 28bp) AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 205 0.1522872806691726 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 193 0.14337290326414787 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 192 0.1426300384803958 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 173 0.12851560758910663 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 170 0.12628701323785047 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 167 0.12405841888659427 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 166 0.1233155541028422 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 165 0.12257268931909013 No Hit ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC 162 0.12034409496783395 RNA PCR Primer, Index 17 (95% over 24bp) ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 161 0.1196012301840819 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 147 0.10920112321155304 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 146 0.10845825842780098 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.10845825842780098 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.10771539364404892 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 137 0.10177247537403243 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0011142971756280921 0.0 14-15 0.0 0.0 0.0 0.002971459135008246 0.0 16-17 0.0 0.0 0.0 0.002971459135008246 0.0 18-19 0.0 0.0 0.0 0.002971459135008246 0.0 20-21 0.0 0.0 0.0 0.0037143239187603073 0.0 22-23 0.0 0.0 0.0 0.014857295675041229 0.0 24-25 0.0 0.0 0.0 0.04531475180887575 0.0 26-27 0.0 0.0 0.0 0.10177247537403242 0.0 28-29 0.0 0.0 0.0 0.2826600502176594 0.0 30-31 0.0 0.0 0.0 0.6797212771331362 0.0 32-33 0.0 0.0 0.0 1.2431842156090749 7.428647837520615E-4 34-35 0.0 0.0 0.0 2.080021394505772 7.428647837520615E-4 36-37 0.0 0.0 0.0 3.1393465761362114 7.428647837520615E-4 38-39 0.0 0.0 0.0 4.359501983448973 7.428647837520615E-4 40-41 0.0 0.0 0.0 5.49757083215713 7.428647837520615E-4 42-43 0.0 0.0 0.0 6.373036979808935 7.428647837520615E-4 44-45 0.0 0.0 0.0 7.198359754557476 7.428647837520615E-4 46-47 0.0 0.0 0.0 8.108740547045628 7.428647837520615E-4 48-49 0.0 0.0 0.0 8.820776442271978 7.428647837520615E-4 50-51 0.0 0.0 0.0 9.456668697163742 7.428647837520615E-4 52-53 0.0 0.0 0.0 9.977045478182061 7.428647837520615E-4 54-55 0.0 0.0 0.0 10.524165391415455 7.428647837520615E-4 56-57 0.0 0.0 0.0 11.086142600323889 7.428647837520615E-4 58-59 0.0 0.0 0.0 11.335002302880829 7.428647837520615E-4 60-61 0.0 0.0 0.0 11.488775313117507 7.428647837520615E-4 62-63 0.0 0.0 0.0 11.609490840477216 7.428647837520615E-4 64-65 0.0 0.0 0.0 11.732434962188183 7.428647837520615E-4 66-67 0.0 0.0 0.0 11.864664893696048 7.428647837520615E-4 68-69 0.0 0.0 0.0 12.016952174365223 7.428647837520615E-4 70-71 0.0 0.0 0.0 12.25764036430089 7.428647837520615E-4 72-73 0.0 0.0 0.0 12.510957255560342 7.428647837520615E-4 74-75 0.0 0.0 0.0 12.867532351761334 7.428647837520615E-4 76-77 0.0 0.0 0.0 13.264222146284933 7.428647837520615E-4 78-79 0.0 0.0 0.0 14.076916219709688 7.428647837520615E-4 80-81 0.0 0.0 0.0 14.823123894988633 7.428647837520615E-4 82-83 0.0 0.0 0.0 15.346472135141962 7.428647837520615E-4 84-85 0.0 0.0 0.0 15.856077376795875 7.428647837520615E-4 86-87 0.0 0.0 0.0 16.242367064346947 7.428647837520615E-4 88-89 0.0 0.0 0.0 16.616028050574236 7.428647837520615E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGCC 20 0.0021237747 70.79153 9 GTACCCA 50 4.394551E-7 56.654274 1 GACCCGG 25 0.00514894 56.612183 6 AGATACG 15 0.009421736 48.603138 66-67 TTACGCG 20 4.873031E-4 48.464035 62-63 GTAAGCG 35 4.0742943E-6 41.031715 60-61 GCGGAGT 25 0.0014414038 38.88251 66-67 TGACTAG 25 0.0015705106 38.209854 76-77 GTAATGG 65 1.3701282E-4 36.316845 1 TGCGGAC 35 1.7904407E-4 34.716526 64-65 TCAACTG 35 1.8208685E-4 34.617165 62-63 TAATGGG 55 0.0026494418 34.335926 2 GGGGCAG 110 7.363451E-8 34.310413 7 TGGGGGA 55 0.0026591218 34.310413 6 ATGGGGG 70 2.1180967E-4 33.722782 5 TGGGGCA 130 8.734787E-9 32.660877 6 CGTCTTA 30 0.0035292297 32.395893 68-69 TATCCAC 30 0.004035376 31.521471 86-87 TACCCAT 90 2.4990524E-5 31.474598 2 GCTTACG 55 2.5630397E-6 30.462942 60-61 >>END_MODULE