FastQCFastQC Report
Fri 27 May 2016
SRR939719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038533
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94220.9072412720635742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58820.5663758397662857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49160.47336001841058495No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48010.46228670634443014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28940.27866230538654047No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26420.25439730851114023No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25520.2457312381984973No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22730.2188664202293042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.17823217943002292No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14490.13952373203355117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.13374635182512254No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13020.12536915052290104No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.12479141250205819No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12300.11843629427278671No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11360.10938506527958186No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.10524460946354135No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10540.10148931232806277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG74850.028.2709671
TACATGG76750.027.939322
ACATGGG77050.026.3566193
CATGGGA53350.024.99264
ATGGGAG16350.023.2173025
CATGGGG21000.022.1435034
TAGGACA3650.022.1001684
GTCTTAG2606.330083E-1021.8980541
GAGTACT60100.021.86663812-13
ATACCGA1100.002894685621.5683467
AGTACTT63500.021.51768112-13
AGTACAT55250.021.2948762
GTATCAA128450.021.1280571
GTACTTT65300.020.16458714-15
TTAGGGT3850.019.7196314
CTTAACG857.983401E-519.55064620-21
ATGGGAC10850.019.2425425
ATGGGGA12400.019.1332115
CATTCCG1751.002303E-418.9783139
ATGGGAT19950.018.7898675
Produced by FastQC (version 0.11.5)