##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939719_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1038533 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.66105747241542 31.0 30.0 33.0 25.0 34.0 2 30.5802983631719 31.0 31.0 34.0 26.0 34.0 3 30.925790514119434 31.0 31.0 34.0 27.0 34.0 4 34.725488742293216 37.0 35.0 37.0 32.0 37.0 5 34.73785907621616 37.0 35.0 37.0 32.0 37.0 6 34.8458440896919 37.0 35.0 37.0 32.0 37.0 7 34.789256576343746 37.0 35.0 37.0 32.0 37.0 8 34.69537799954359 37.0 35.0 37.0 32.0 37.0 9 36.05201760560329 38.0 35.0 39.0 32.0 39.0 10-11 35.93918633302938 38.0 35.0 39.0 30.5 39.0 12-13 35.84013411225257 38.0 35.0 39.0 30.0 39.0 14-15 36.576728905099785 39.0 36.0 40.0 30.5 41.0 16-17 36.467509458052845 38.0 36.0 40.0 30.0 41.0 18-19 36.409789096735494 38.5 36.0 40.0 30.0 41.0 20-21 36.653127536631 39.0 36.0 40.0 30.5 41.0 22-23 36.58129062822269 39.0 36.0 40.0 30.0 41.0 24-25 36.379666317777094 39.0 35.5 40.5 30.0 41.0 26-27 36.27393207534089 39.0 35.5 40.0 29.5 41.0 28-29 36.33123213224809 39.0 35.5 40.0 30.0 41.0 30-31 36.101496534053325 38.5 35.0 40.0 29.5 41.0 32-33 35.73917246731688 38.0 35.0 40.0 28.5 41.0 34-35 35.53482556644806 38.0 35.0 40.0 27.5 41.0 36-37 35.306940174265044 38.0 34.5 40.0 27.0 41.0 38-39 35.069132613022404 38.0 34.0 40.0 25.5 41.0 40-41 34.84070799868661 38.0 34.0 40.0 25.0 41.0 42-43 34.410269582189486 38.0 33.0 40.0 24.0 41.0 44-45 33.98501251284264 38.0 33.0 40.0 22.5 41.0 46-47 33.50124502543491 38.0 33.0 40.0 20.0 41.0 48-49 33.07032467913875 37.0 32.5 40.0 16.5 41.0 50-51 32.41483900848601 36.5 31.5 39.5 14.5 40.5 52-53 32.59173853888129 37.0 32.0 39.0 14.5 40.0 54-55 32.72078884349366 37.0 32.0 40.0 12.5 41.0 56-57 32.433668453481985 37.0 31.5 40.0 8.5 41.0 58-59 32.073641376826735 36.0 31.0 39.0 7.5 40.5 60-61 31.66125149610075 36.0 31.0 39.0 6.5 40.0 62-63 31.270960576120352 35.0 30.0 39.0 2.0 40.0 64-65 30.75654456815527 35.0 29.5 38.5 2.0 40.0 66-67 30.1623477539953 35.0 29.0 38.0 2.0 40.0 68-69 29.625440404878805 34.0 28.0 37.5 2.0 39.5 70-71 29.053852886716165 34.0 27.5 37.0 2.0 39.0 72-73 28.398776928609877 33.5 26.0 36.0 2.0 39.0 74-75 28.063865568065722 33.0 26.0 36.0 2.0 38.5 76-77 27.52486247427862 33.0 26.0 35.0 2.0 38.0 78-79 26.85798188406146 32.5 24.5 35.0 2.0 37.0 80-81 26.116367510709818 32.0 22.5 35.0 2.0 36.5 82-83 25.371636240735732 31.5 19.5 34.5 2.0 36.0 84-85 24.595830849862256 31.0 16.5 34.0 2.0 35.5 86-87 23.703857749344508 30.5 7.5 34.0 2.0 35.0 88-89 22.725256684188178 29.5 2.0 34.0 2.0 35.0 90-91 21.2925501645109 28.0 2.0 34.0 2.0 35.0 92-93 19.715030239770908 25.5 2.0 33.0 2.0 34.5 94-95 19.183176653991737 25.0 2.0 32.5 2.0 34.0 96-97 18.870757116047347 25.0 2.0 32.5 2.0 34.0 98-99 17.981509494642925 24.5 2.0 32.0 2.0 34.0 100-101 16.179509461904438 17.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.535498174717297 1101 2 0.7620170598405842 1101 3 0.5574909709192895 1101 4 0.40468731454596707 1101 5 0.5465506606767789 1101 6 0.500024135114991 1101 7 0.49799442187924114 1101 8 0.6551198706014816 1101 9 0.7295552037594248 1101 10-11 0.7253483583950739 1101 12-13 0.6401780734103255 1101 14-15 0.6372784309427288 1101 16-17 0.5977922349771276 1101 18-19 0.7973582344317904 1101 20-21 0.511975726529009 1101 22-23 0.5071061353271915 1101 24-25 0.6555530379632799 1101 26-27 0.5156582670164838 1101 28-29 0.5651540258317809 1101 30-31 0.5918339287191472 1101 32-33 0.5347166311005083 1101 34-35 0.5829438361414887 1101 36-37 0.3036077365989769 1101 38-39 0.496965939346552 1101 40-41 0.3415939169296749 1101 42-43 0.30533321751168785 1101 44-45 0.3475711079052388 1101 46-47 0.26546346790869535 1101 48-49 0.609982239401937 1101 50-51 0.3589390765865801 1101 52-53 0.42749002119249013 1101 54-55 0.20110632856413702 1101 56-57 0.3699710085819845 1101 58-59 -0.0032925227022104764 1101 60-61 0.23285434857223564 1101 62-63 -0.1310064666916979 1101 64-65 0.16370047429302303 1101 66-67 0.7028218151548131 1101 68-69 0.43578209622666364 1101 70-71 0.12445733457299823 1101 72-73 0.20813745087734858 1101 74-75 0.43188089717205 1101 76-77 -0.04269688885417722 1101 78-79 -0.6288355880571928 1101 80-81 -0.7021459514000732 1101 82-83 -0.5367530868050139 1101 84-85 -0.3310290252094319 1101 86-87 -0.9869309039927927 1101 88-89 -4.9981445845817305 1101 90-91 -8.559005207287775 1101 92-93 -11.659586711726227 1101 94-95 -9.176195551939474 1101 96-97 -5.613441301677714 1101 98-99 -4.062693416508395 1101 100-101 -3.317422547984105 1102 1 1.6688374246602429 1102 2 0.8881422722046075 1102 3 0.9992588538718472 1102 4 0.7093060735793628 1102 5 0.7520302864089601 1102 6 0.801676137042115 1102 7 0.8823035731838544 1102 8 0.6086343367119866 1102 9 0.8989646415969901 1102 10-11 0.7915013365627814 1102 12-13 0.8452298014829509 1102 14-15 0.8777030322055026 1102 16-17 1.0175376517177526 1102 18-19 1.1047187080986518 1102 20-21 1.207360137107905 1102 22-23 1.2392643877297616 1102 24-25 1.2885119344310638 1102 26-27 1.072622539146714 1102 28-29 0.8391891958497908 1102 30-31 1.2432589864060901 1102 32-33 1.3156552166927185 1102 34-35 1.3601327703910542 1102 36-37 1.1995525346312874 1102 38-39 1.2405569832900625 1102 40-41 1.01266740122108 1102 42-43 1.2215476861578765 1102 44-45 0.9946664751999066 1102 46-47 1.1025200391489065 1102 48-49 0.6031865906456915 1102 50-51 0.7848018450527405 1102 52-53 0.7812567884415174 1102 54-55 0.74602290436858 1102 56-57 0.8664083643646237 1102 58-59 0.6585542202042021 1102 60-61 0.41535111556769877 1102 62-63 0.2774614695103459 1102 64-65 0.4638849457284735 1102 66-67 0.579026267826162 1102 68-69 0.7199568559700538 1102 70-71 0.8942103583831269 1102 72-73 0.9057564381228325 1102 74-75 0.6976655663913114 1102 76-77 0.7690408193893461 1102 78-79 0.8533797173666464 1102 80-81 0.8984068289838305 1102 82-83 0.40826047913559904 1102 84-85 0.6357502683473797 1102 86-87 0.20527438545619958 1102 88-89 -0.4805215402325125 1102 90-91 -6.010266079057942 1102 92-93 -11.016158585854585 1102 94-95 -8.609728782154754 1102 96-97 -5.025449694811066 1102 98-99 -3.3030451166884287 1102 100-101 -2.574440069600861 1103 1 1.8995576959658784 1103 2 1.0905262840021734 1103 3 0.7340333382686524 1103 4 0.6431116779044999 1103 5 0.7782673858212448 1103 6 0.5466437970070004 1103 7 0.3956310781855663 1103 8 0.6045056682856114 1103 9 0.7265212816919089 1103 10-11 0.5426686040759918 1103 12-13 0.525286819859005 1103 14-15 0.6667914827419423 1103 16-17 0.6487513697530005 1103 18-19 0.5185702825888185 1103 20-21 0.4579557797895504 1103 22-23 0.5841252883510961 1103 24-25 0.5543264878071312 1103 26-27 0.6441975336826573 1103 28-29 0.44910928846261555 1103 30-31 0.6245357512977563 1103 32-33 0.6332180293751009 1103 34-35 0.6777093933372242 1103 36-37 0.5737890672223003 1103 38-39 0.4956529706278445 1103 40-41 0.3183137575931383 1103 42-43 0.5244914339512405 1103 44-45 0.40211308952368086 1103 46-47 0.7341024311826629 1103 48-49 0.8202575177587192 1103 50-51 0.6461305845719494 1103 52-53 0.6340507849397099 1103 54-55 0.44617805968000823 1103 56-57 0.47534972958725774 1103 58-59 0.48608504111029305 1103 60-61 0.3271905946685649 1103 62-63 0.5232043055861162 1103 64-65 0.35517415186647483 1103 66-67 0.36440876409739076 1103 68-69 0.42540074186604926 1103 70-71 0.026411210056089374 1103 72-73 0.06870314011174727 1103 74-75 -0.05327773467003638 1103 76-77 -0.21158398096227415 1103 78-79 -0.6935595679101709 1103 80-81 -1.0378245781979878 1103 82-83 -1.3513370537508393 1103 84-85 -1.4766953011999107 1103 86-87 -1.1326963944274127 1103 88-89 -1.9755144189399694 1103 90-91 -7.794587586802496 1103 92-93 -10.871474456609729 1103 94-95 -9.664237893326177 1103 96-97 -6.542649477689702 1103 98-99 -5.110334894451263 1103 100-101 -3.9475603726100594 1104 1 1.8279741679389119 1104 2 1.091446545813092 1104 3 0.9432751923465155 1104 4 0.7374777494923066 1104 5 0.7614066200064755 1104 6 0.7118279385418376 1104 7 0.8546391215591527 1104 8 1.0413917591347115 1104 9 0.7376892651414622 1104 10-11 0.8179702751715681 1104 12-13 0.7232387022937772 1104 14-15 0.8825119086305477 1104 16-17 0.8673328111398106 1104 18-19 0.9119515313626962 1104 20-21 0.8817959110516966 1104 22-23 0.8346951016235593 1104 24-25 0.8336908651144697 1104 26-27 0.7449879601062932 1104 28-29 0.7985027360558448 1104 30-31 0.9879514207168896 1104 32-33 1.048888161885671 1104 34-35 0.9159865776214318 1104 36-37 1.130432251756197 1104 38-39 0.8891444368046564 1104 40-41 0.866047880401716 1104 42-43 1.3926011519873072 1104 44-45 0.9899228307446322 1104 46-47 1.0100143999365656 1104 48-49 0.9272821829153983 1104 50-51 0.8350603343534715 1104 52-53 0.7267161556304487 1104 54-55 0.6347054624372248 1104 56-57 0.8577623849706129 1104 58-59 0.44718008132716136 1104 60-61 0.7840659847989322 1104 62-63 0.7895245067596299 1104 64-65 0.7031704422839837 1104 66-67 0.7141125325338358 1104 68-69 0.6322046155262306 1104 70-71 0.6746889293762379 1104 72-73 0.6380188291488622 1104 74-75 0.43808500186043275 1104 76-77 0.6543959373555737 1104 78-79 0.45255303931994817 1104 80-81 0.43076310527475314 1104 82-83 0.01741185388411992 1104 84-85 -0.04420827582137221 1104 86-87 0.21082093181966854 1104 88-89 0.2924315780994995 1104 90-91 -3.0772117597261115 1104 92-93 -8.260913203912256 1104 94-95 -5.707888265176152 1104 96-97 -2.6987576262870157 1104 98-99 -1.7214996676851975 1104 100-101 -1.0296744779407998 1105 1 1.7956762437675593 1105 2 1.0556261918078569 1105 3 0.8230585390695069 1105 4 0.5666067307554243 1105 5 0.5418456279625232 1105 6 0.5473196844666361 1105 7 0.4564252153212962 1105 8 0.5197114004646934 1105 9 0.6094232688214589 1105 10-11 0.47901725990735855 1105 12-13 0.5140381943506895 1105 14-15 0.7791475631255906 1105 16-17 0.45372822928582224 1105 18-19 0.5130176935416628 1105 20-21 0.49744159483120853 1105 22-23 0.42741026476139865 1105 24-25 0.547097452825362 1105 26-27 0.35535570053701093 1105 28-29 0.5129796324199063 1105 30-31 0.5367694164149199 1105 32-33 0.652125521618224 1105 34-35 0.6742554246424532 1105 36-37 0.6218832585719625 1105 38-39 0.3624224838153083 1105 40-41 0.7549482872745017 1105 42-43 0.5099940563333263 1105 44-45 0.5281543594978331 1105 46-47 0.557361433911737 1105 48-49 0.3692818200989052 1105 50-51 0.2951489652395196 1105 52-53 0.16518040524819355 1105 54-55 0.040664982482837786 1105 56-57 0.29234605304535677 1105 58-59 0.1991478424902482 1105 60-61 0.3206425520366061 1105 62-63 0.231863118209084 1105 64-65 0.32757762605053387 1105 66-67 0.4179692718501862 1105 68-69 0.26529607119783094 1105 70-71 0.4779060750470592 1105 72-73 0.5661241495935734 1105 74-75 0.24890470797907582 1105 76-77 0.2269613091129017 1105 78-79 -0.0555963374131494 1105 80-81 -0.06234234474727529 1105 82-83 -0.3641840203740472 1105 84-85 -0.6587173073604902 1105 86-87 -0.509173366778306 1105 88-89 -0.7287154403263969 1105 90-91 -3.7617702898010066 1105 92-93 -8.911512110279688 1105 94-95 -5.986377393639106 1105 96-97 -3.0998880588161057 1105 98-99 -1.8414975944480965 1105 100-101 -1.2855679135538303 1106 1 1.5023260076148972 1106 2 1.0535273916169494 1106 3 0.7093062787941946 1106 4 0.8653319430067583 1106 5 0.9179750128213513 1106 6 0.7583650345100708 1106 7 0.774042640481909 1106 8 -0.2249817516350845 1106 9 -0.6118255022361794 1106 10-11 -0.3561012078781616 1106 12-13 -0.43681389744848786 1106 14-15 0.4882484365215589 1106 16-17 0.8083411481989558 1106 18-19 0.8825051436499862 1106 20-21 0.4784658213691344 1106 22-23 0.5436843975760084 1106 24-25 0.6217764974948778 1106 26-27 0.7030933173900493 1106 28-29 0.4937682332549045 1106 30-31 0.5097266620182026 1106 32-33 0.8190004623353957 1106 34-35 0.6039328206848467 1106 36-37 0.5507431162329581 1106 38-39 0.5032995534445917 1106 40-41 0.3717155026303729 1106 42-43 0.6575160719041975 1106 44-45 0.602892875191408 1106 46-47 0.38844114164553645 1106 48-49 0.3288091534332409 1106 50-51 0.07362015341035999 1106 52-53 0.16863499422813533 1106 54-55 0.2932158377363834 1106 56-57 0.4491575931340961 1106 58-59 0.03443568977468203 1106 60-61 -0.032912686005577285 1106 62-63 0.22418458092526805 1106 64-65 0.40433815656218286 1106 66-67 0.18258898099923826 1106 68-69 0.2742475895156602 1106 70-71 -0.01706448303204411 1106 72-73 -0.18673986953351474 1106 74-75 -0.14949619899168098 1106 76-77 0.07155975925284608 1106 78-79 0.27354717444868726 1106 80-81 0.11722024963474453 1106 82-83 -0.30460534623159674 1106 84-85 -0.37511956635604804 1106 86-87 0.13697930964362115 1106 88-89 0.03006406370031911 1106 90-91 -3.7685627841060914 1106 92-93 -9.323181830795985 1106 94-95 -6.499428648628275 1106 96-97 -3.2990700687560484 1106 98-99 -2.0104547738051775 1106 100-101 -1.245863100616548 1107 1 1.9149522474108878 1107 2 1.0901425829465197 1107 3 0.9944839068967042 1107 4 0.8548290413814428 1107 5 0.9260493717437512 1107 6 0.8021115370500667 1107 7 0.7803711920697367 1107 8 0.8311038677446234 1107 9 0.9520328566737533 1107 10-11 0.7941598059059203 1107 12-13 0.705641642917854 1107 14-15 0.7783649847487055 1107 16-17 0.7808290951139512 1107 18-19 0.946984667044589 1107 20-21 0.8310019962117394 1107 22-23 0.9931775678841888 1107 24-25 0.9047718664291722 1107 26-27 0.802702537123281 1107 28-29 0.5487954569380307 1107 30-31 0.9576844571258931 1107 32-33 1.255535689454021 1107 34-35 1.0697001887400006 1107 36-37 0.8754071652277986 1107 38-39 0.8543463364048876 1107 40-41 0.8782155174385551 1107 42-43 1.2077314141213975 1107 44-45 0.8905941301463116 1107 46-47 0.9120921547593497 1107 48-49 0.6764710965479779 1107 50-51 0.850206694794565 1107 52-53 0.707241497048706 1107 54-55 0.6138871318316603 1107 56-57 0.7059752045890875 1107 58-59 0.5527565348701344 1107 60-61 0.5820198899799074 1107 62-63 0.35355981571297335 1107 64-65 0.38987055765518264 1107 66-67 0.47704510745804285 1107 68-69 0.695599748471416 1107 70-71 0.6834469449915943 1107 72-73 0.5030855029703325 1107 74-75 0.3276586650301887 1107 76-77 0.22484320198490693 1107 78-79 0.10768222823358187 1107 80-81 -0.030243575522650445 1107 82-83 0.021647855064664157 1107 84-85 0.26541636532053303 1107 86-87 0.41775500925252373 1107 88-89 0.2660169393723386 1107 90-91 -3.43031417178911 1107 92-93 -4.994440074669683 1107 94-95 -2.5559062952799874 1107 96-97 -1.0887353569623386 1107 98-99 -0.5450724186175577 1107 100-101 -0.2597804584618473 1108 1 1.6503855154566445 1108 2 0.9905600209903973 1108 3 -0.3547744530435537 1108 4 -0.03610381079138136 1108 5 0.415839244119951 1108 6 0.5064024066269468 1108 7 0.4825469229775905 1108 8 0.5010371492056365 1108 9 0.6848523857088367 1108 10-11 0.609707863824049 1108 12-13 0.6208628157689589 1108 14-15 0.5608097809709705 1108 16-17 0.5407016054660545 1108 18-19 0.460231673206664 1108 20-21 0.49126399615808225 1108 22-23 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0.011054153154614 2311 74-75 0.16845505397781935 2311 76-77 0.37979372937171263 2311 78-79 0.2586326537633781 2311 80-81 0.5080679452799828 2311 82-83 0.9509806805891436 2311 84-85 1.1049699880532486 2311 86-87 1.7113761033960806 2311 88-89 2.201403614039947 2311 90-91 2.5375017814540968 2311 92-93 3.7674442578403067 2311 94-95 3.5874148189028254 2311 96-97 2.537876225729498 2311 98-99 2.1448399732839256 2311 100-101 1.7763621723496748 2312 1 1.2147792376183304 2312 2 0.5075425867987349 2312 3 -0.1638036247282848 2312 4 -0.43992662052546905 2312 5 0.07551646306051651 2312 6 -0.03959413329773298 2312 7 0.2723784115746355 2312 8 0.3248457171932486 2312 9 0.25820693828860186 2312 10-11 0.1165109262774493 2312 12-13 0.1437093490028829 2312 14-15 0.1790067185175701 2312 16-17 0.27646702765259334 2312 18-19 -0.06988746999457618 2312 20-21 0.3273763816984072 2312 22-23 0.4156398360006506 2312 24-25 0.4188194231615441 2312 26-27 0.18708962132478035 2312 28-29 0.34813015667483427 2312 30-31 0.24882810057389548 2312 32-33 0.247772908879611 2312 34-35 0.32476216975310734 2312 36-37 0.24596932808813676 2312 38-39 0.27219274404405525 2312 40-41 0.36548394547407526 2312 42-43 0.2917527644588631 2312 44-45 0.07477534742372427 2312 46-47 -0.023822842708383973 2312 48-49 0.04199648774612541 2312 50-51 -0.2096946884906714 2312 52-53 -0.12173713335275238 2312 54-55 0.006104976879697688 2312 56-57 -0.08900139320223843 2312 58-59 -0.11654380083911065 2312 60-61 -0.001636066394883784 2312 62-63 -0.1363694734739731 2312 64-65 -0.15587450046228568 2312 66-67 -0.12969355031680507 2312 68-69 -0.2832663179294457 2312 70-71 -0.00637776787673161 2312 72-73 0.08226382542115473 2312 74-75 0.2464675600221966 2312 76-77 0.511791896140533 2312 78-79 0.5323855793210939 2312 80-81 0.635492894420441 2312 82-83 0.7159184777299643 2312 84-85 0.9730791015944718 2312 86-87 1.3998297444976906 2312 88-89 1.762227605703636 2312 90-91 2.2903843481670663 2312 92-93 3.4327462558572 2312 94-95 3.2961227458922657 2312 96-97 2.3288606566745003 2312 98-99 2.24433772789126 2312 100-101 2.277221203655806 2313 1 -7.644102055797493 2313 2 -2.7432603078699778 2313 3 -1.563525346685882 2313 4 -0.8702509122269007 2313 5 -0.46459220323265527 2313 6 -0.2094976256390808 2313 7 -0.16606579616249206 2313 8 -0.03031625232921442 2313 9 0.07664574396925161 2313 10-11 -0.1268962004296199 2313 12-13 -0.24645818688192378 2313 14-15 -0.20217729363946546 2313 16-17 -0.25416319356722994 2313 18-19 -0.34905516858226093 2313 20-21 -0.3528839910114314 2313 22-23 -1.1619245578749897 2313 24-25 -0.3908005654091866 2313 26-27 -0.3453519719452558 2313 28-29 -0.513291065071563 2313 30-31 -0.20324868557118947 2313 32-33 -0.2582631278062877 2313 34-35 -0.16136039601531849 2313 36-37 -0.31405841956689784 2313 38-39 -0.4089769214051415 2313 40-41 -0.2875289032450965 2313 42-43 -0.05256862423833297 2313 44-45 -0.08387639866680274 2313 46-47 -1.039504836307671 2313 48-49 -0.22700749005895915 2313 50-51 0.03191990122955701 2313 52-53 -0.5563798642606059 2313 54-55 -0.42975522046367587 2313 56-57 0.003752477403281773 2313 58-59 -0.2631150578912411 2313 60-61 -0.19572257784460234 2313 62-63 -0.3423577412332328 2313 64-65 -0.16928386333220402 2313 66-67 0.15531662462486295 2313 68-69 -0.04919497070946477 2313 70-71 0.28145606358391717 2313 72-73 0.14344331716298697 2313 74-75 0.28026808307591367 2313 76-77 0.5253523402633107 2313 78-79 0.4681079200305618 2313 80-81 0.6632120964870047 2313 82-83 0.752694940793603 2313 84-85 0.8281120308956531 2313 86-87 1.3347714319430395 2313 88-89 2.069397433054231 2313 90-91 2.867469850538278 2313 92-93 3.7009054610335532 2313 94-95 3.3481111274633975 2313 96-97 2.0994483827854324 2313 98-99 1.9672971892244782 2313 100-101 1.7264482847900453 2314 1 0.6799385605779307 2314 2 -0.02082841327037599 2314 3 -1.0889750172259554 2314 4 -0.22035626910974315 2314 5 -0.07367381590427158 2314 6 -3.1465705285852223 2314 7 -2.106689368321497 2314 8 -2.0590170069824083 2314 9 -2.7278490367227377 2314 10-11 -2.064435217623128 2314 12-13 -0.7748815324273863 2314 14-15 -0.7285399455868458 2314 16-17 -1.7134116701184254 2314 18-19 -2.76940569988556 2314 20-21 -2.330295023441934 2314 22-23 -3.713155283590602 2314 24-25 -2.488140199513687 2314 26-27 -1.1386901365764643 2314 28-29 -1.133377827594309 2314 30-31 -1.4464615767682432 2314 32-33 -1.783064255183504 2314 34-35 -1.0450385311566563 2314 36-37 -1.840606429334379 2314 38-39 -3.0535827996330056 2314 40-41 -2.1387623068163606 2314 42-43 -1.158791207599137 2314 44-45 -1.6212395979009386 2314 46-47 -1.1110370550951671 2314 48-49 -1.7964166714217562 2314 50-51 -2.776527227021319 2314 52-53 -1.7769878881203027 2314 54-55 -2.1131297537218074 2314 56-57 -0.8583990457107973 2314 58-59 -0.5457886624355623 2314 60-61 -1.0327880330047456 2314 62-63 -0.5628582324523776 2314 64-65 -0.771611003566484 2314 66-67 -0.447685000498776 2314 68-69 -0.258057250267548 2314 70-71 -0.3914154177556135 2314 72-73 -1.3046221831859732 2314 74-75 -1.6105737343177644 2314 76-77 -0.46258182800309555 2314 78-79 -1.460567394994051 2314 80-81 -0.5789702387035298 2314 82-83 -0.13289032966144276 2314 84-85 -0.9043388095326002 2314 86-87 0.2369811649440976 2314 88-89 1.4349109202415313 2314 90-91 2.42981869813984 2314 92-93 3.547719984383619 2314 94-95 3.3702770775247366 2314 96-97 2.4365699055480405 2314 98-99 2.517371834450447 2314 100-101 2.3995327393359567 2315 1 -2.3975081870128108 2315 2 -1.5338033901007755 2315 3 -2.1547295927513233 2315 4 -3.76421839679497 2315 5 -3.4370296783785577 2315 6 -2.442702523732791 2315 7 -5.145037884336777 2315 8 -2.363912009129834 2315 9 -2.1514289096855066 2315 10-11 -3.465561297929348 2315 12-13 -2.5398117975780536 2315 14-15 -1.6018728645045428 2315 16-17 -2.203594728807211 2315 18-19 -1.5080403510606217 2315 20-21 -0.9592187860345192 2315 22-23 -0.6212435465863066 2315 24-25 -1.7259686239054375 2315 26-27 -3.1266099207789466 2315 28-29 -2.4970727574073663 2315 30-31 -2.504556055998485 2315 32-33 -3.3681070530164874 2315 34-35 -2.8072185834483747 2315 36-37 -2.427969012876808 2315 38-39 -1.0075299201978183 2315 40-41 -0.5608832483686825 2315 42-43 -1.9048260034429347 2315 44-45 -5.4241218586512545 2315 46-47 -2.14194492673181 2315 48-49 -0.808310400182954 2315 50-51 -2.1195145609725117 2315 52-53 -1.8342827335432688 2315 54-55 -0.24852405136265077 2315 56-57 0.2447311109383108 2315 58-59 -1.1227126812123558 2315 60-61 -1.472857880876532 2315 62-63 -3.043146175971291 2315 64-65 -2.7509759304409585 2315 66-67 -2.4604640717556627 2315 68-69 -1.0399926843114145 2315 70-71 -2.2366641424856795 2315 72-73 -1.292249050881896 2315 74-75 -0.47807752056971253 2315 76-77 -2.4299183410345826 2315 78-79 -0.8994712279619463 2315 80-81 -0.2747816268452148 2315 82-83 -0.09513706519645382 2315 84-85 -1.4451003547754837 2315 86-87 -1.4521590733072216 2315 88-89 -0.8002690114929152 2315 90-91 0.24334578962020714 2315 92-93 0.9618681342061741 2315 94-95 1.044599131217936 2315 96-97 0.5913513201369796 2315 98-99 0.08216477732890226 2315 100-101 -0.35139027730848227 2316 1 -6.739401427325188 2316 2 -5.219375246314129 2316 3 -2.854994718964594 2316 4 -3.529672409972939 2316 5 -1.5853931528101626 2316 6 -2.246704981201269 2316 7 -2.9723677376308544 2316 8 -2.1326770075217567 2316 9 -6.160724820227056 2316 10-11 -6.270301820594959 2316 12-13 -5.155184994678564 2316 14-15 -3.9800395352944804 2316 16-17 -4.229786765949619 2316 18-19 -3.5953454235064584 2316 20-21 -2.6292469479635514 2316 22-23 -2.9292002192729356 2316 24-25 -2.839499750465059 2316 26-27 -3.566309169519286 2316 28-29 -3.6049251113817817 2316 30-31 -6.02450958675395 2316 32-33 -4.336989770745728 2316 34-35 -4.774681865735637 2316 36-37 -3.4074619332234874 2316 38-39 -5.590207653719169 2316 40-41 -3.042587271854824 2316 42-43 -3.916369095729941 2316 44-45 -2.302476138192418 2316 46-47 -3.2238289309210515 2316 48-49 -4.542586312868604 2316 50-51 -3.593696710515239 2316 52-53 -5.473688405512462 2316 54-55 -4.51618710604545 2316 56-57 -3.5152482314827047 2316 58-59 -2.251093762003695 2316 60-61 -2.5939273744566016 2316 62-63 -3.466081242727025 2316 64-65 -2.4600411440675387 2316 66-67 -3.27644668805247 2316 68-69 -2.6851224848276516 2316 70-71 -1.7885235307574519 2316 72-73 -1.3290461365551884 2316 74-75 -1.335509301079842 2316 76-77 -2.011305491278268 2316 78-79 -3.9251117778411313 2316 80-81 -4.80825432667946 2316 82-83 -2.4611184445124863 2316 84-85 -1.0179214131362357 2316 86-87 -1.124448848424116 2316 88-89 -0.09357261605489597 2316 90-91 0.17656428990471795 2316 92-93 0.18386288940768125 2316 94-95 -0.47585846920428665 2316 96-97 -2.374900751805825 2316 98-99 -4.619088902688912 2316 100-101 -4.0318925739694595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6094.0 3 1626.0 4 1414.0 5 1566.0 6 1815.0 7 2003.0 8 2330.0 9 2922.0 10 3411.0 11 4383.0 12 5982.0 13 7648.0 14 8920.0 15 11331.0 16 13785.0 17 15669.0 18 12477.0 19 10380.0 20 10680.0 21 11580.0 22 13132.0 23 15047.0 24 17290.0 25 20302.0 26 24231.0 27 28515.0 28 34021.0 29 40442.0 30 48323.0 31 57530.0 32 67264.0 33 78870.0 34 94390.0 35 110399.0 36 120005.0 37 100689.0 38 31422.0 39 645.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.4482436139084 20.29106790781082 13.07192819232271 21.188760285958068 2 15.652133676389923 21.27516409743758 39.56230400610792 23.51039822006457 3 20.88321433873645 26.451987945651318 28.63659763501854 24.028200080593695 4 12.286610116218306 16.28608562012985 37.59181091916011 33.83549334449173 5 12.891744552040604 39.049083451025 32.985062396668326 15.074109600266073 6 30.49137462253127 36.294269417222985 17.77197996089366 15.442375999352084 7 28.087646712766063 34.28097483821813 20.259815409541996 17.371563039473813 8 26.927260524427783 32.8409590594552 19.98021245573839 20.251567960378626 9 27.46197088830874 17.137475733024207 21.06060065406311 34.33995272460394 10-11 25.074470413861672 27.19773305213459 26.35490829700219 21.37288823700155 12-13 27.001551012497295 24.946230441790266 27.162534249316217 20.889684296396222 14-15 23.337449260073374 25.646257552227347 27.41496496962234 23.60132821807694 16-17 21.08327957626898 29.40839265895458 27.647137665333105 21.861190099443338 18-19 20.82955010663757 27.71086777375573 31.13247987918106 20.327102240425642 20-21 22.04769441012718 26.283522022040845 30.794257459545005 20.874526108286968 22-23 21.22367035503989 26.568442967530125 30.967043897252942 21.24084278017704 24-25 21.332052525967953 26.69725872807337 31.176208633291374 20.794480112667305 26-27 21.11002860988023 26.57731929909895 31.7119459399207 20.600706151100123 28-29 20.405871814258976 27.551186334034107 31.541194425800622 20.501747425906295 30-31 20.539015386723445 27.17517010987484 31.265333771423858 21.02048073197786 32-33 20.340338070436566 27.264389222466946 31.19786552931158 21.197407177784907 34-35 21.34908865310155 27.093888365191 30.93914416078994 20.61787882091751 36-37 21.16028424773689 26.648152963840865 31.303435761777276 20.888127026644966 38-39 20.758004665890947 27.112531332673395 31.314052281906264 20.815411719529397 40-41 20.65247229221635 27.375864353073364 31.287060502423543 20.684602852286744 42-43 20.339803132228475 27.526948115380627 30.577699092845272 21.555549659545626 44-45 20.609738861820148 27.310470176068208 30.46702139821342 21.612769563898226 46-47 21.13658599655346 27.816281713080443 29.92521241757542 21.121919872790674 48-49 21.048054323537787 27.729521290714903 29.76766745655227 21.45475692919504 50-51 20.44887347266175 28.042164357208804 30.22673404804181 21.282228122087638 52-53 20.975014828746676 28.495180235030205 29.28529060723778 21.244514328985336 54-55 20.165297632262234 27.902578160442467 29.942182025270025 21.98994218202527 56-57 20.78980050893237 28.09762160482569 29.314417724047487 21.79816016219445 58-59 21.244863810150598 27.535236819605625 29.31941745136904 21.900481918874735 60-61 20.675932826371653 27.783525916064317 30.156116220120737 21.384425037443293 62-63 20.573804966892528 27.69113969964037 30.025737393693376 21.709317939773726 64-65 20.221178561075643 28.166937335795804 29.752269014405837 21.85961508872272 66-67 20.45960256403454 27.999569734590878 29.167026427075076 22.373801274299502 68-69 20.45402690569116 27.796630445426228 29.43399066033217 22.315351988550443 70-71 20.768356141981727 27.951090488000073 29.064950739783622 22.215602630234578 72-73 20.80692201680802 27.45948549082585 29.44858575603278 22.285006736333347 74-75 20.160856020012663 28.445708318534873 29.092288330579535 22.30114733087293 76-77 20.451267963459177 27.820919466559584 29.166055336334644 22.561757233646592 78-79 20.702359424595564 27.8376775335618 28.97938441358002 22.480578628262617 80-81 20.774347570685173 28.016026976136683 28.82649847448325 22.383126978694897 82-83 20.836050420776488 27.838244202407125 28.81103793142562 22.514667445390764 84-85 20.50399236030117 27.847117234537112 28.75898805838637 22.889902346775347 86-87 20.530646930433058 27.809163219147376 28.921473770462537 22.738716079957033 88-89 20.74839616295197 27.633868637373094 28.365837957203343 23.251897242471593 90-91 20.638004021436053 27.478604083914355 28.713324222720633 23.170067671928965 92-93 21.324468033588705 27.397262927485983 28.700665381747037 22.577603657178276 94-95 20.791875455599875 27.030206181134673 28.916419855724897 23.26149850754056 96-97 20.68990653140867 26.997564081645265 28.889142060479905 23.423387326466163 98-99 21.058860796779737 27.395182545055597 27.858221070594908 23.687735587569765 100-101 21.084236098701272 26.97023409917459 28.362368328270122 23.583161473854013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2874.0 1 1901.5 2 1158.0 3 1478.5 4 1348.0 5 1181.5 6 1814.0 7 3397.0 8 4858.0 9 4876.5 10 3804.0 11 2924.0 12 2598.5 13 2501.0 14 2507.0 15 2464.0 16 2409.5 17 2534.0 18 2651.0 19 2699.5 20 2972.0 21 3384.5 22 3788.0 23 4260.5 24 4872.0 25 5611.5 26 6890.0 27 8436.0 28 9658.0 29 10964.5 30 12624.5 31 14431.5 32 16936.5 33 19394.5 34 21512.0 35 23454.0 36 25793.5 37 28112.5 38 28957.0 39 29135.5 40 29337.0 41 30099.0 42 30850.0 43 31614.0 44 32736.0 45 34148.5 46 35725.0 47 39618.5 48 47060.0 49 46842.0 50 39496.5 51 34760.0 52 32171.0 53 30887.5 54 29243.5 55 26726.5 56 24307.0 57 22284.5 58 20112.0 59 17902.5 60 15491.0 61 12685.5 62 10182.0 63 7858.5 64 5736.0 65 4364.0 66 3311.5 67 2478.5 68 1812.5 69 1308.5 70 1037.5 71 874.5 72 783.5 73 706.5 74 586.5 75 496.0 76 382.5 77 246.0 78 167.0 79 126.0 80 80.5 81 52.5 82 40.5 83 26.5 84 17.5 85 9.0 86 3.0 87 2.0 88 2.5 89 2.0 90 1.5 91 1.5 92 2.0 93 2.5 94 2.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12690978524514868 2 0.11477728680744859 3 0.11853258394292718 4 0.12970218567922254 5 0.11728081823110098 6 0.1310502410611892 7 0.15820392804080372 8 0.14607142960310363 9 0.15695216232897752 10-11 0.1486712506968965 12-13 0.14140138060129048 14-15 0.14486780872634766 16-17 0.14058291840509643 18-19 0.15497822409109774 20-21 0.15791505903038228 22-23 0.19137571940419804 24-25 0.21255944683510297 26-27 0.22652144900547214 28-29 0.22093664813732447 30-31 0.21352234353650776 32-33 0.2128483158455244 34-35 0.20220830729500172 36-37 0.21024849475173155 38-39 0.1998010655414898 40-41 0.2057228802551291 42-43 0.19744196862304808 44-45 0.19012395369237184 46-47 0.20519328706935647 48-49 0.2424573894137211 50-51 0.24452761732174133 52-53 0.24443132765160086 54-55 0.24293883776442346 56-57 0.21626659913551133 58-59 0.21751836484733755 60-61 0.18925734666110752 62-63 0.1838169802981706 64-65 0.18338367678253845 66-67 0.18906476732082658 68-69 0.19127942973405757 70-71 0.19715309961262664 72-73 0.22733991120166622 74-75 0.23003602196559955 76-77 0.24467205182695206 78-79 0.24038716150570086 80-81 0.2556009293878962 82-83 0.25521577070733426 84-85 0.22786950438743883 86-87 0.23123964284235554 88-89 0.23340616042051626 90-91 0.4404289512225418 92-93 0.5818784766589026 94-95 0.33667683164617784 96-97 0.26807044167108796 98-99 0.2975350807340739 100-101 0.31660043542188837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1038533.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.486949364507176 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84782482937672 45.66846987367549 2 11.030490885338896 12.461575602115921 3 3.4261794289813317 5.806032717557538 4 1.487538574936315 3.361060646407152 5 0.7723783586904761 2.1814648618795043 6 0.4736062985243975 1.6051545002075587 7 0.3282523733444837 1.297938263432241 8 0.22513099038056322 1.017357028320658 9 0.1613967348268371 0.8205128268984306 >10 1.1076207355188166 11.995167274613486 >50 0.08748361580375318 3.419783005997331 >100 0.046695795355870116 4.7327083605096565 >500 0.0022650943864606695 0.8895357550440193 >1k 0.0027878084756439007 3.2369928576723557 >5k 3.484760594554876E-4 1.5062464256686958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9422 0.9072412720635742 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5882 0.5663758397662857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4916 0.47336001841058495 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4801 0.46228670634443014 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2894 0.27866230538654047 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2642 0.25439730851114023 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2552 0.2457312381984973 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2273 0.2188664202293042 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1851 0.17823217943002292 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1449 0.13952373203355117 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.13374635182512254 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1302 0.12536915052290104 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1296 0.12479141250205819 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1230 0.11843629427278671 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1136 0.10938506527958186 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1093 0.10524460946354135 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1054 0.10148931232806277 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.81448350702385E-5 0.0 18-19 0.0 0.0 0.0 2.88869010421431E-4 0.0 20-21 0.0 0.0 0.0 4.81448350702385E-4 0.0 22-23 0.0 0.0 0.0 6.258828559131005E-4 0.0 24-25 0.0 0.0 0.0 0.001348055381966678 0.0 26-27 0.0 0.0 0.0 0.002696110763933356 0.0 28-29 0.0 0.0 0.0 0.007992042621659591 0.0 30-31 0.0 0.0 0.0 0.02662409379384189 0.0 32-33 0.0 0.0 0.0 0.06985815568691606 0.0 34-35 0.0 0.0 0.0 0.13702020060989878 0.0 36-37 0.0 0.0 0.0 0.24963096983918664 0.0 38-39 0.0 0.0 0.0 0.4367699437572037 0.0 40-41 0.0 0.0 0.0 0.652218080696521 0.0 42-43 0.0 0.0 0.0 0.8531264774446262 0.0 44-45 0.0 0.0 0.0 1.0885547209380926 0.0 46-47 0.0 0.0 0.0 1.3762201104827674 0.0 48-49 0.0 0.0 0.0 1.6949870634828166 0.0 50-51 0.0 0.0 0.0 2.055158574643271 0.0 52-53 0.0 0.0 0.0 2.3841322326782106 0.0 54-55 0.0 0.0 0.0 2.7193647192722814 0.0 56-57 0.0 0.0 0.0 3.1198816022215956 0.0 58-59 0.0 0.0 0.0 3.565702774972004 0.0 60-61 0.0 0.0 0.0 4.054902444120697 0.0 62-63 0.0 0.0 0.0 4.513578287834859 0.0 64-65 0.0 0.0 0.0 4.945100444569407 0.0 66-67 0.0 0.0 0.0 5.429341195705866 0.0 68-69 0.0 0.0 0.0 5.991143276140479 0.0 70-71 0.0 0.0 0.0 6.59882738439703 0.0 72-73 0.0 0.0 0.0 7.177046853590594 0.0 74-75 0.0 0.0 0.0 7.715546833851211 0.0 76-77 0.0 0.0 0.0 8.273978775830908 0.0 78-79 0.0 0.0 0.0 8.91223485435706 0.0 80-81 0.0 0.0 0.0 9.595313774333604 0.0 82-83 0.0 0.0 0.0 10.209930738840267 0.0 84-85 0.0 0.0 0.0 10.783095000351457 0.0 86-87 0.0 0.0 0.0 11.342056535516926 0.0 88-89 0.0 0.0 0.0 11.95835856925105 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7485 0.0 28.270967 1 TACATGG 7675 0.0 27.93932 2 ACATGGG 7705 0.0 26.356619 3 CATGGGA 5335 0.0 24.9926 4 ATGGGAG 1635 0.0 23.217302 5 CATGGGG 2100 0.0 22.143503 4 TAGGACA 365 0.0 22.100168 4 GTCTTAG 260 6.330083E-10 21.898054 1 GAGTACT 6010 0.0 21.866638 12-13 ATACCGA 110 0.0028946856 21.568346 7 AGTACTT 6350 0.0 21.517681 12-13 AGTACAT 5525 0.0 21.294876 2 GTATCAA 12845 0.0 21.128057 1 GTACTTT 6530 0.0 20.164587 14-15 TTAGGGT 385 0.0 19.719631 4 CTTAACG 85 7.983401E-5 19.550646 20-21 ATGGGAC 1085 0.0 19.242542 5 ATGGGGA 1240 0.0 19.133211 5 CATTCCG 175 1.002303E-4 18.978313 9 ATGGGAT 1995 0.0 18.789867 5 >>END_MODULE