FastQCFastQC Report
Fri 27 May 2016
SRR939719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038533
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99510.9581785075678867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73040.703299750706044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65230.6280975183263314No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64970.6255939869026791No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31980.30793436510924543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25600.2465015555596211No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22890.22040705495155183No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20410.19652721675671356No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18000.17332140625285858No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.1665811293430252No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16400.15791505903038228No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14790.14241242213776548No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13940.13422780017582492No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13260.1276801026062725No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12150.11699194922067954No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12090.11641421119983669No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11800.11362181076576285No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11660.11227375538379618No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.11044425165112712No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11410.10986651363028424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG73500.025.2922671
TACATGG74700.024.7572562
CTAGACG1000.001720364923.5888634
ACATGGG76250.023.140293
CGCATAG2250.022.69913966-67
CGGCTAA1050.00229173622.4536889
CATGGGA51300.021.611634
AGTACTT69100.021.35862412-13
GTAAGGT3101.2732926E-1121.3060724
TACCTGG6100.020.8819472
GAGTACT67950.020.54041512-13
GTACTTT71250.019.39053214-15
TAAGGTG3900.019.3549675
CATGGGG21750.019.304914
GTATCAA147650.019.0454711
AACGCGT901.05430445E-418.8550862-63
AGTACAT57300.018.8546262
TACTTTT79200.018.78369914-15
ATGGGAT21100.018.5580645
AGGACCG2051.6851069E-518.410825
Produced by FastQC (version 0.11.5)