##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939719_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1038533 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99600205289577 31.0 31.0 33.0 30.0 34.0 2 31.18784766589025 31.0 31.0 34.0 28.0 34.0 3 31.221870657937686 31.0 31.0 34.0 28.0 34.0 4 35.06931508194732 35.0 35.0 37.0 32.0 37.0 5 34.8027284640931 35.0 35.0 37.0 32.0 37.0 6 34.87352351827048 35.0 35.0 37.0 32.0 37.0 7 34.78001950828717 35.0 35.0 37.0 32.0 37.0 8 34.72375841692079 35.0 35.0 37.0 31.0 37.0 9 36.215752412296965 38.0 35.0 39.0 32.0 39.0 10-11 36.05924173810558 37.5 35.0 39.0 30.5 39.0 12-13 35.98398750930399 37.0 35.0 39.0 30.0 39.0 14-15 36.786442992182245 38.0 36.0 40.0 31.0 41.0 16-17 36.73557364089538 38.0 36.0 40.0 31.0 41.0 18-19 36.78814106051517 38.5 36.0 40.0 31.0 41.0 20-21 36.73522603518617 38.5 35.0 40.0 30.5 41.0 22-23 36.55967985610472 38.0 35.0 40.0 30.0 41.0 24-25 36.33717898227596 38.0 34.5 40.0 30.0 41.0 26-27 35.92698643182258 38.0 34.0 40.0 29.0 41.0 28-29 35.653494400274234 38.0 34.0 40.0 27.5 41.0 30-31 35.300753081510166 37.5 34.0 40.0 27.0 41.0 32-33 35.12467875358799 37.0 33.5 40.0 27.0 41.0 34-35 35.06148095438469 38.0 33.0 40.0 27.0 41.0 36-37 34.91818411162669 38.0 33.0 40.0 26.0 41.0 38-39 34.70285344808494 37.0 33.0 40.0 25.0 41.0 40-41 34.44653756789626 37.0 33.0 40.0 25.0 41.0 42-43 34.1149857539433 37.0 33.0 40.0 24.0 41.0 44-45 33.70880559404468 37.0 33.0 40.0 23.5 41.0 46-47 33.212740952863314 37.0 32.0 40.0 21.0 41.0 48-49 32.73478888008373 36.5 31.0 39.5 18.5 41.0 50-51 32.358954409729876 36.0 31.0 39.0 15.5 40.0 52-53 32.023033933442655 36.0 31.0 39.0 13.0 40.0 54-55 31.64571515782358 35.5 30.0 39.0 8.5 40.0 56-57 30.28701736006463 34.5 28.0 38.5 4.5 40.0 58-59 26.901270349618166 32.5 17.5 37.5 2.0 40.0 60-61 26.852490965621698 32.5 18.0 37.0 2.0 40.0 62-63 28.36154652765006 33.5 25.5 37.0 2.0 39.0 64-65 27.7122489126489 33.0 23.5 37.0 2.0 39.0 66-67 22.345159470137204 25.0 12.5 34.0 2.0 37.5 68-69 15.326728664375615 12.5 2.0 27.0 2.0 34.0 70-71 14.160113352199689 10.5 2.0 23.5 2.0 31.5 72-73 15.850898815925927 13.0 2.0 28.0 2.0 33.0 74-75 16.497665938395794 15.0 2.0 28.0 2.0 34.0 76-77 17.7941928662835 20.0 2.0 29.5 2.0 34.0 78-79 20.548176610661386 25.0 2.0 32.5 2.0 35.0 80-81 22.73454863735673 29.5 2.0 34.0 2.0 35.0 82-83 23.442970998514248 31.0 2.0 34.0 2.0 35.0 84-85 23.350584430152917 31.0 2.0 34.0 2.0 35.0 86-87 23.002901207761333 31.0 2.0 34.0 2.0 35.0 88-89 22.433179301957665 30.5 2.0 34.0 2.0 35.0 90-91 20.857031986465522 29.5 2.0 34.0 2.0 35.0 92-93 18.79754182101098 26.0 2.0 33.5 2.0 35.0 94-95 14.760137617196566 2.0 2.0 31.0 2.0 34.0 96-97 13.463097946815363 2.0 2.0 30.0 2.0 34.0 98-99 12.418737295781646 2.0 2.0 29.0 2.0 34.0 100-101 11.780890929801942 2.0 2.0 28.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17215673765047157 1101 2 0.3422031099534344 1101 3 -0.006084460656801127 1101 4 0.2803769905729183 1101 5 0.34786419386309575 1101 6 0.318914568493895 1101 7 0.06108377422012978 1101 8 0.11604766396762756 1101 9 0.19163089478906414 1101 10-11 0.3075075988041007 1101 12-13 0.49713254796787254 1101 14-15 0.16928031652849995 1101 16-17 -0.13906084006872277 1101 18-19 0.19456006234624112 1101 20-21 0.18032045915273187 1101 22-23 0.4502853253222341 1101 24-25 0.3873805781083135 1101 26-27 0.4533934166395852 1101 28-29 0.245256571203619 1101 30-31 0.09672657380122729 1101 32-33 -0.17004207338217014 1101 34-35 0.25612515377553535 1101 36-37 -0.03464555812750092 1101 38-39 -0.09568788508559578 1101 40-41 0.23805347259450826 1101 42-43 0.24089821482555607 1101 44-45 -0.196987563316668 1101 46-47 -0.43777342357115145 1101 48-49 -0.5562544110599887 1101 50-51 -0.6647394732466765 1101 52-53 -0.7804780750004028 1101 54-55 -0.6758382254243323 1101 56-57 -0.3894430055911684 1101 58-59 -8.8497647139593 1101 60-61 -10.143305395725093 1101 62-63 -4.151714750523194 1101 64-65 -1.7440223534413626 1101 66-67 -3.5080191759087533 1101 68-69 -5.315112774856534 1101 70-71 -5.407209298481964 1101 72-73 -7.275095547132992 1101 74-75 -8.053987015543466 1101 76-77 -9.471506829200582 1101 78-79 -11.83051769030051 1101 80-81 -9.29391697436209 1101 82-83 -7.496314183711608 1101 84-85 -7.011479879720536 1101 86-87 -6.733000599249337 1101 88-89 -6.661056302430492 1101 90-91 -10.634132485927365 1101 92-93 -13.077263780087513 1101 94-95 -10.703884215016961 1101 96-97 -10.131815126113892 1101 98-99 -9.560618680414612 1101 100-101 -9.09291840096458 1102 1 0.5390520390172071 1102 2 0.5625989311365878 1102 3 0.5124387535836341 1102 4 0.3628312847636508 1102 5 0.48212263137101985 1102 6 0.6256379500898532 1102 7 0.5919431195050109 1102 8 0.7040868150186697 1102 9 0.7712578948144255 1102 10-11 0.7618746906214326 1102 12-13 0.7420992036465464 1102 14-15 0.7098830500021407 1102 16-17 0.925149165763365 1102 18-19 1.0019110271762202 1102 20-21 0.9223647322972397 1102 22-23 0.9327034859328265 1102 24-25 0.8002130704378487 1102 26-27 0.9886965583592868 1102 28-29 0.5955114080319746 1102 30-31 0.7168580493191357 1102 32-33 0.4787173403663374 1102 34-35 0.6762699416650761 1102 36-37 0.819390626166431 1102 38-39 0.5727801779009383 1102 40-41 0.43320967108830644 1102 42-43 0.6056413494453352 1102 44-45 0.5224732222400377 1102 46-47 0.01718955535726252 1102 48-49 0.23039264627195166 1102 50-51 0.27106002975811805 1102 52-53 0.41017410442501046 1102 54-55 0.055416687474721726 1102 56-57 0.1263923774247786 1102 58-59 -9.242714228374691 1102 60-61 -10.00904695821717 1102 62-63 -3.7466319602501024 1102 64-65 -1.2223802407045916 1102 66-67 -2.1688865086166196 1102 68-69 -3.811293392043442 1102 70-71 -3.95922897543508 1102 72-73 -6.538144840943373 1102 74-75 -7.333302759641647 1102 76-77 -8.371502391743373 1102 78-79 -10.791322264684972 1102 80-81 -8.088766988308386 1102 82-83 -4.882011625200565 1102 84-85 -4.1597543427882115 1102 86-87 -3.8107226622865866 1102 88-89 -4.086399254480593 1102 90-91 -8.945616625187451 1102 92-93 -12.790645249266204 1102 94-95 -10.307330851424396 1102 96-97 -9.894956338680771 1102 98-99 -9.18700063137437 1102 100-101 -8.93697981537237 1103 1 0.05293435635545407 1103 2 0.1476593480384274 1103 3 0.23044277417173475 1103 4 0.040324397645143506 1103 5 0.23782146674546567 1103 6 0.19717751829694663 1103 7 0.15592907386629662 1103 8 0.18215971880076154 1103 9 0.27675317890956563 1103 10-11 0.39625816441915873 1103 12-13 0.04116707088547855 1103 14-15 0.2536459746077142 1103 16-17 0.2747728060528303 1103 18-19 0.34307017665611284 1103 20-21 0.2780847033797471 1103 22-23 0.1352646293746318 1103 24-25 0.13914696610910937 1103 26-27 0.19848647422475807 1103 28-29 0.4026792952057221 1103 30-31 0.22318975504283145 1103 32-33 0.2508840146386575 1103 34-35 0.18516735422209507 1103 36-37 0.050607466522187394 1103 38-39 -0.06213949439951705 1103 40-41 0.132187951746495 1103 42-43 0.25914903635825226 1103 44-45 0.07546920885729946 1103 46-47 0.02772498528879197 1103 48-49 0.032011209187906786 1103 50-51 0.054061756641537784 1103 52-53 -0.050333945157440496 1103 54-55 0.013566784626927841 1103 56-57 0.49393273341735267 1103 58-59 -6.274788265799305 1103 60-61 -8.77199858386535 1103 62-63 -3.3060479181900426 1103 64-65 -1.4743370950387344 1103 66-67 -2.807085818343605 1103 68-69 -4.9525099441849925 1103 70-71 -5.045914800340148 1103 72-73 -7.351775331123674 1103 74-75 -7.704736409129948 1103 76-77 -8.862334778373116 1103 78-79 -11.248494234893231 1103 80-81 -9.182003081372752 1103 82-83 -6.294686325037485 1103 84-85 -5.320164759810144 1103 86-87 -4.9956787216298935 1103 88-89 -4.914012336523168 1103 90-91 -9.383051326657583 1103 92-93 -12.799727599268724 1103 94-95 -10.377174759416814 1103 96-97 -9.818377225083546 1103 98-99 -9.381208366563163 1103 100-101 -8.99976067410658 1104 1 0.1660580744985225 1104 2 0.11454918413908644 1104 3 0.06356720569605301 1104 4 0.1279254748508336 1104 5 0.05954749267687731 1104 6 0.28387973512917597 1104 7 0.19211747785409727 1104 8 0.1745714824575515 1104 9 0.04157064248253306 1104 10-11 0.22390846493100725 1104 12-13 0.16529861838775162 1104 14-15 0.13727316156710145 1104 16-17 0.2896343209179051 1104 18-19 0.2951512881041012 1104 20-21 0.08447410253439358 1104 22-23 0.15513657744048004 1104 24-25 0.3389047731751518 1104 26-27 0.3726007222317591 1104 28-29 0.36404399353462935 1104 30-31 0.2516023034189985 1104 32-33 0.1803316792067946 1104 34-35 0.2692846340993924 1104 36-37 0.11265344394367105 1104 38-39 0.2541134705912995 1104 40-41 0.23076011917707717 1104 42-43 0.12112510241735208 1104 44-45 -0.12115721121725187 1104 46-47 -0.09015056535235999 1104 48-49 0.0773023579100709 1104 50-51 -0.18921979710282244 1104 52-53 0.05658085928692458 1104 54-55 -0.23355240354521456 1104 56-57 0.4357650531548316 1104 58-59 -6.201043192588557 1104 60-61 -8.538643373507053 1104 62-63 -2.980601965736902 1104 64-65 -0.8042445338217057 1104 66-67 -2.554576321455734 1104 68-69 -4.346975889147629 1104 70-71 -4.999717300741425 1104 72-73 -7.591739305370611 1104 74-75 -8.163010739889163 1104 76-77 -8.93087323853969 1104 78-79 -11.506533506530975 1104 80-81 -9.006310120662224 1104 82-83 -6.325771912952806 1104 84-85 -5.397960768442058 1104 86-87 -5.303745280100401 1104 88-89 -5.418610435362144 1104 90-91 -9.772588520572441 1104 92-93 -13.257628118253583 1104 94-95 -10.604331400104515 1104 96-97 -9.981826423421367 1104 98-99 -9.210018981676145 1104 100-101 -8.823959636136863 1105 1 0.11386122063582249 1105 2 0.2389807284415717 1105 3 -0.06589712166808681 1105 4 0.06457875186122664 1105 5 2.010806728307557E-4 1105 6 0.023103826144286188 1105 7 0.1990980671717395 1105 8 0.1948259723116479 1105 9 0.12569845754978104 1105 10-11 0.09066514464179676 1105 12-13 0.05618259485650867 1105 14-15 0.13762509356492814 1105 16-17 -0.030683402667754933 1105 18-19 0.2022293195654754 1105 20-21 0.3178604761835544 1105 22-23 0.3754885094383056 1105 24-25 0.2887028917124823 1105 26-27 0.23349376911282604 1105 28-29 0.36671432292017414 1105 30-31 0.257235288621267 1105 32-33 0.22947206327396685 1105 34-35 0.04831954222907342 1105 36-37 0.09041642111214543 1105 38-39 0.2975959906109935 1105 40-41 0.08833184022302021 1105 42-43 0.375655902946022 1105 44-45 0.4842249122958435 1105 46-47 -0.041797493074128056 1105 48-49 0.041942262541162734 1105 50-51 -0.08217908390925999 1105 52-53 0.3515107579574632 1105 54-55 0.028941182377508312 1105 56-57 0.32868080198215566 1105 58-59 -6.510140038676617 1105 60-61 -8.468130506479302 1105 62-63 -2.947851557568349 1105 64-65 -0.6494312547380794 1105 66-67 -1.907444385829745 1105 68-69 -4.045016680087583 1105 70-71 -4.392245354230942 1105 72-73 -6.28168825190701 1105 74-75 -6.930593086275252 1105 76-77 -8.61528923356392 1105 78-79 -11.375234364332826 1105 80-81 -8.324620950734527 1105 82-83 -5.317660372796791 1105 84-85 -4.501423074400023 1105 86-87 -4.511776482318766 1105 88-89 -4.632230566494389 1105 90-91 -8.99177425670119 1105 92-93 -10.656630891208206 1105 94-95 -9.9987181106547 1105 96-97 -9.820924565282617 1105 98-99 -9.250413933343804 1105 100-101 -8.7067823484493 1106 1 0.11305121023215747 1106 2 0.1724288885285361 1106 3 0.28728548147649846 1106 4 0.2568884709321537 1106 5 0.07004114125799532 1106 6 0.0682144798889297 1106 7 0.09468802725839964 1106 8 0.2935584114215999 1106 9 0.4111879793575852 1106 10-11 0.2836922799903192 1106 12-13 0.153730883069656 1106 14-15 0.20760743292917283 1106 16-17 -0.037099498086377025 1106 18-19 0.11295051488789198 1106 20-21 0.18125026433119729 1106 22-23 0.04621250516843389 1106 24-25 0.22785198933379291 1106 26-27 0.16362510221228632 1106 28-29 0.11327893028343539 1106 30-31 0.1443367010204497 1106 32-33 0.28156566916024417 1106 34-35 0.317478621948851 1106 36-37 0.497190697711126 1106 38-39 0.5345737183360839 1106 40-41 0.3986185206709081 1106 42-43 0.40360728425466164 1106 44-45 0.38683847560388074 1106 46-47 0.3440655669885899 1106 48-49 0.4435869751154442 1106 50-51 0.7096971742040168 1106 52-53 0.35365999559915906 1106 54-55 0.5439075549009935 1106 56-57 1.4921982646320942 1106 58-59 -6.295130455560969 1106 60-61 -8.329348473052068 1106 62-63 -2.1000175826003833 1106 64-65 0.01864214003359166 1106 66-67 -1.28392382165298 1106 68-69 -3.775682198486713 1106 70-71 -3.787343737371886 1106 72-73 -5.5572927861585555 1106 74-75 -6.608209698414267 1106 76-77 -8.363814879125652 1106 78-79 -10.486670265860855 1106 80-81 -7.6720687404449865 1106 82-83 -4.57492374210884 1106 84-85 -3.7112895431310626 1106 86-87 -3.4843912049353705 1106 88-89 -3.876013586086703 1106 90-91 -8.591826350307075 1106 92-93 -11.196356782483129 1106 94-95 -9.903618673596874 1106 96-97 -9.900115879803032 1106 98-99 -9.215801797340347 1106 100-101 -8.883881788518316 1107 1 0.08881410465601647 1107 2 0.014565819056315377 1107 3 0.012317708768144087 1107 4 -0.15019638933311796 1107 5 -0.05002831693381182 1107 6 0.12712300737837978 1107 7 0.22672561823133464 1107 8 0.0969368603653109 1107 9 0.16257410361853886 1107 10-11 0.2159752192440152 1107 12-13 0.10532759534034142 1107 14-15 0.1722236592730937 1107 16-17 0.1551785778468897 1107 18-19 -0.0210098022878924 1107 20-21 2.3810135385105013E-4 1107 22-23 0.014752901123770812 1107 24-25 0.10581148348611435 1107 26-27 0.10668996051352764 1107 28-29 0.14405579895976217 1107 30-31 0.09273562423142323 1107 32-33 -0.014955809725620384 1107 34-35 -0.16490119402435255 1107 36-37 0.14046380588727203 1107 38-39 0.09562241856693277 1107 40-41 0.22269647720342078 1107 42-43 0.2616536634970146 1107 44-45 0.45290948943473097 1107 46-47 0.35485761795369797 1107 48-49 0.30718564518435443 1107 50-51 0.02727667468489159 1107 52-53 0.37470165025040814 1107 54-55 -0.04135954582741519 1107 56-57 0.22002144552423175 1107 58-59 -6.240026852806956 1107 60-61 -8.957654400847346 1107 62-63 -3.354836088781628 1107 64-65 -1.1127353175409525 1107 66-67 -2.327790348447227 1107 68-69 -4.255507991854827 1107 70-71 -4.7366935020775855 1107 72-73 -6.257202534154384 1107 74-75 -6.958269788479221 1107 76-77 -8.674305934201197 1107 78-79 -11.477824322446835 1107 80-81 -8.501206420734665 1107 82-83 -5.525471411222238 1107 84-85 -4.818982474325988 1107 86-87 -4.441544104923992 1107 88-89 -4.613753576141836 1107 90-91 -8.861297168046349 1107 92-93 -10.952539980022788 1107 94-95 -9.872384309873503 1107 96-97 -10.01657837534109 1107 98-99 -9.287821026161133 1107 100-101 -8.63423679532229 1108 1 -0.08330362762913524 1108 2 -0.10587634777580845 1108 3 -0.1954398865025304 1108 4 -0.027961049971864327 1108 5 -0.21350215271319684 1108 6 0.024447111015703626 1108 7 -4.002526423576569E-4 1108 8 0.00935871650010256 1108 9 -0.26467049226923933 1108 10-11 -0.06930075492714849 1108 12-13 -0.18376166530018878 1108 14-15 -0.002780029922256233 1108 16-17 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4.607442172022438 2311 80-81 2.7221388734161955 2311 82-83 1.5314096386135176 2311 84-85 1.3530302090858228 2311 86-87 1.277864194429327 2311 88-89 1.3531445568856313 2311 90-91 2.921936725537435 2311 92-93 4.506594323978309 2311 94-95 7.5181481919228315 2311 96-97 8.264451199726969 2311 98-99 8.815843943995873 2311 100-101 8.266875549636396 2312 1 -0.32703821500081887 2312 2 -0.28262693560628094 2312 3 -0.5321759458399562 2312 4 -0.35358090048131885 2312 5 -0.39169603162967803 2312 6 -0.36177184866772905 2312 7 -0.29382085251619117 2312 8 -0.29179926174538906 2312 9 -0.3652133991148361 2312 10-11 -0.3867543019822577 2312 12-13 -0.6326192191607589 2312 14-15 -0.6076420570293735 2312 16-17 -0.6027363085863939 2312 18-19 -0.5250727565519 2312 20-21 -0.30948624997272134 2312 22-23 -0.5449807974902257 2312 24-25 -0.6344262661855922 2312 26-27 -0.6833274764262711 2312 28-29 -0.8307597762269907 2312 30-31 -0.5735667814771048 2312 32-33 -0.3615707887253805 2312 34-35 -0.4556420543600126 2312 36-37 -0.5281425645440336 2312 38-39 -0.49491484085370985 2312 40-41 -0.6219408423797717 2312 42-43 -0.6482209242309693 2312 44-45 -0.7047985103703951 2312 46-47 -0.4633053511526697 2312 48-49 -0.5854706726188823 2312 50-51 -0.5723965668070932 2312 52-53 -0.46353192769860385 2312 54-55 -0.3530202019050215 2312 56-57 1.0531938922135353 2312 58-59 4.390632363221886 2312 60-61 3.96012629252877 2312 62-63 1.6437833451919666 2312 64-65 1.3757527898769446 2312 66-67 0.41997281734872516 2312 68-69 -0.7720566560991315 2312 70-71 0.5579868150277232 2312 72-73 6.491260828444453 2312 74-75 4.98420303923951 2312 76-77 5.43373114677124 2312 78-79 5.506787209953131 2312 80-81 3.6901212226498927 2312 82-83 2.407467749833227 2312 84-85 1.8184583403495935 2312 86-87 1.6408763100049306 2312 88-89 1.8135896755131995 2312 90-91 3.343139337812982 2312 92-93 4.596466431649876 2312 94-95 7.895924760167704 2312 96-97 8.411870122238247 2312 98-99 8.649978724351268 2312 100-101 8.059603285619556 2313 1 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3.9273543248256075 2313 60-61 3.6872070260222394 2313 62-63 1.2395327923628194 2313 64-65 1.4109751598408664 2313 66-67 1.9920173354235722 2313 68-69 0.6639129449101091 2313 70-71 2.4203012996723405 2313 72-73 7.500293643933773 2313 74-75 9.254327502941138 2313 76-77 8.307932159376303 2313 78-79 6.407808551282148 2313 80-81 3.969430384585749 2313 82-83 2.698085964702024 2313 84-85 2.4379608061399054 2313 86-87 2.2425792401797437 2313 88-89 2.2527039306104903 2313 90-91 3.531913159158801 2313 92-93 5.146184586967816 2313 94-95 8.14768143038498 2313 96-97 8.461095359940751 2313 98-99 8.523867555799896 2313 100-101 7.66996713896895 2314 1 -0.2646137549693641 2314 2 -0.25758685833426753 2314 3 -0.27904267015720663 2314 4 -0.2939850277239131 2314 5 -0.5250998267914113 2314 6 -0.43117674964189234 2314 7 -0.5461652254312028 2314 8 -0.4834309924935525 2314 9 -0.5467883052640659 2314 10-11 -0.503490840485938 2314 12-13 -0.24870002438099448 2314 14-15 -0.44112330963165647 2314 16-17 -0.23579580163208647 2314 18-19 -0.5936192200592458 2314 20-21 -0.5150173349331766 2314 22-23 -0.33318484685396044 2314 24-25 -0.5672654395908054 2314 26-27 -0.5213280630462762 2314 28-29 -0.7241892107286745 2314 30-31 -0.7081286454453206 2314 32-33 -0.41368093506010695 2314 34-35 -0.7087298164215454 2314 36-37 -0.5945191240176868 2314 38-39 -0.25088513225888676 2314 40-41 -0.6653713725797417 2314 42-43 -0.5212408709968841 2314 44-45 -0.5445838209334326 2314 46-47 -0.245986659719847 2314 48-49 -0.419908132689109 2314 50-51 0.006600329838214236 2314 52-53 -0.29955941455443735 2314 54-55 -0.1355295701253887 2314 56-57 1.1473582031288814 2314 58-59 4.189908865213528 2314 60-61 3.8280082571663705 2314 62-63 1.6254025849796356 2314 64-65 1.7226382253495842 2314 66-67 2.2858488112734996 2314 68-69 0.15507392922289398 2314 70-71 2.3080732066235488 2314 72-73 7.842349952543245 2314 74-75 9.794352239632612 2314 76-77 8.662151790401271 2314 78-79 6.76287170683376 2314 80-81 4.446868195572659 2314 82-83 3.4637175285864537 2314 84-85 2.9141165937390916 2314 86-87 2.6710800761727995 2314 88-89 2.783264539688755 2314 90-91 3.691881560462246 2314 92-93 5.756468724187837 2314 94-95 8.622202470798841 2314 96-97 9.313074378941812 2314 98-99 9.24307430801289 2314 100-101 8.735407654590055 2315 1 -0.6377554775821395 2315 2 -0.4781313251320398 2315 3 -0.44155199450902316 2315 4 -0.45150629478088433 2315 5 -0.7875002728533289 2315 6 -0.8795518636061885 2315 7 -0.6143347138916084 2315 8 -0.7736349603348955 2315 9 -0.9945474468962558 2315 10-11 -0.8904523827148907 2315 12-13 -0.7681898033844448 2315 14-15 -0.8303509519080805 2315 16-17 -1.145320391548978 2315 18-19 -0.7748752008303583 2315 20-21 -0.74241472938904 2315 22-23 -0.9875678573198385 2315 24-25 -0.9432694556550629 2315 26-27 -0.853884367061589 2315 28-29 -0.7794440009966834 2315 30-31 -0.9447712760050777 2315 32-33 -1.0114208628764274 2315 34-35 -1.145681186630334 2315 36-37 -1.1457003969518311 2315 38-39 -1.1254396354498937 2315 40-41 -0.3808646778835083 2315 42-43 -0.4469860656592175 2315 44-45 -0.8423744204798354 2315 46-47 -0.6909409609774144 2315 48-49 -0.8473524314601804 2315 50-51 -0.9477564970942751 2315 52-53 -0.737660788153903 2315 54-55 -0.7220096367332829 2315 56-57 0.4027700945971944 2315 58-59 3.8179237389674547 2315 60-61 3.623940579325552 2315 62-63 1.3523412966744424 2315 64-65 1.5246407947265261 2315 66-67 2.0121988643940476 2315 68-69 5.662310191344552 2315 70-71 4.169171445733836 2315 72-73 8.153068436234108 2315 74-75 9.569831266434 2315 76-77 8.242393017975733 2315 78-79 6.2272621014327925 2315 80-81 3.565516334092969 2315 82-83 2.3148330601310185 2315 84-85 1.5908497929524543 2315 86-87 1.6600716899072907 2315 88-89 2.203912422024853 2315 90-91 3.384428483614517 2315 92-93 4.956295234732831 2315 94-95 7.726541025769192 2315 96-97 8.508853744057054 2315 98-99 8.409625126672143 2315 100-101 7.471627392830069 2316 1 -0.2897783534799814 2316 2 -0.34601353157588477 2316 3 -0.6418624766841319 2316 4 -0.4509517150657203 2316 5 -0.7391549408064577 2316 6 -0.532137565757715 2316 7 -0.49498462359201767 2316 8 -0.6271970526466504 2316 9 -0.5216352642849102 2316 10-11 -0.5963488639169086 2316 12-13 -0.6828432838360214 2316 14-15 -0.8718465029005458 2316 16-17 -0.9217969342701693 2316 18-19 -0.8574333595432435 2316 20-21 -0.539582868062837 2316 22-23 -0.7022582410312808 2316 24-25 -2.096526219878278 2316 26-27 -1.5199170810798819 2316 28-29 -1.441424572151945 2316 30-31 -0.5990062858596943 2316 32-33 -0.652799519185443 2316 34-35 -0.7457601472366235 2316 36-37 -0.7550917611766934 2316 38-39 -1.2648947866501743 2316 40-41 -1.0269448919636517 2316 42-43 -1.0494585840696118 2316 44-45 -0.817862615559946 2316 46-47 -0.706151701269178 2316 48-49 -0.7222926440559405 2316 50-51 -0.9968409251555954 2316 52-53 -0.8672200931356358 2316 54-55 -0.9982655845491841 2316 56-57 0.3920619643348715 2316 58-59 3.700311606659991 2316 60-61 4.021728857695706 2316 62-63 1.6669374636833432 2316 64-65 1.32104097450107 2316 66-67 6.238003520389693 2316 68-69 8.207172391685958 2316 70-71 4.814470300289637 2316 72-73 8.388914283689637 2316 74-75 9.58513684191869 2316 76-77 8.362102863518064 2316 78-79 6.277289727336822 2316 80-81 3.6282405368588577 2316 82-83 2.542177757194427 2316 84-85 2.2398193879649497 2316 86-87 2.227215006500149 2316 88-89 2.3041769297477366 2316 90-91 3.6867548321297328 2316 92-93 5.048849228943762 2316 94-95 8.409467804753712 2316 96-97 8.66350932737596 2316 98-99 8.533707699262298 2316 100-101 7.734297168441756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 23.0 4 54.0 5 122.0 6 300.0 7 634.0 8 1277.0 9 2099.0 10 3520.0 11 5084.0 12 6896.0 13 9119.0 14 11859.0 15 16303.0 16 21197.0 17 26045.0 18 26820.0 19 25999.0 20 27288.0 21 26767.0 22 25921.0 23 24949.0 24 24637.0 25 24721.0 26 31350.0 27 44439.0 28 59880.0 29 73994.0 30 80878.0 31 81985.0 32 81455.0 33 78100.0 34 71940.0 35 60529.0 36 41100.0 37 17935.0 38 3242.0 39 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50663327956083 21.654106373245373 15.15671875376207 20.68254159343173 2 16.434602081455633 23.560044063383483 37.437409966644324 22.56794388851656 3 21.274456843583085 26.910110898948787 29.847772728892174 21.967659528575954 4 13.432794143277105 17.963319413056684 36.73306481353987 31.87082163012634 5 12.601806162223195 39.89125261801037 32.92681516991296 14.580126049853467 6 30.19788490110569 37.76018672492833 16.741884947324735 15.300043426641233 7 27.30023985756832 34.834713966720365 20.182796309794682 17.682249865916635 8 26.74681834244717 33.98829693095343 19.33124769506158 19.933637031537817 9 26.707576937853684 17.208697268165768 20.94155891050164 35.14216688347891 10-11 25.177148920640942 27.82867756729926 26.124783709328447 20.86938980273135 12-13 26.940068346407863 25.357162458968563 27.18772537800917 20.515043816614398 14-15 22.830095023689722 26.33875461529536 27.480897287080158 23.350253073934756 16-17 20.654952707328512 29.700982058345765 27.932140817865196 21.711924416460526 18-19 20.465661552831065 27.801419805541045 31.629935798213328 20.102982843414562 20-21 21.692281323751867 26.28346908571995 31.554750787890224 20.469498802637954 22-23 20.993073884026796 26.645181231602656 31.498325041187908 20.863419843182644 24-25 21.16952470455922 26.921580729740896 31.703662762762473 20.205231802937412 26-27 20.992121093947468 26.64514591502914 32.188207879868955 20.17452511115444 28-29 20.1429441487505 27.646380537641395 32.09011884729915 20.120556466308944 30-31 20.399007176543943 27.09315062557026 31.77304691355647 20.734795284329326 32-33 20.349175903271064 27.270298142279813 31.591064686562078 20.789461267887045 34-35 21.276405212772715 26.93009130248063 31.646391135128056 20.14711234961859 36-37 21.108371656273324 26.558399345099076 31.86661209158982 20.46661690703778 38-39 20.586032961344458 27.093262065561536 32.034718961801914 20.28598601129209 40-41 20.565585627579882 27.238226677273524 31.700474419303454 20.495713275843137 42-43 20.312841597155405 27.51128986122789 31.05908481095923 21.116783730657477 44-45 20.70563262743104 27.104307790543636 30.891592303502836 21.298467278522494 46-47 21.158245007440083 27.770864734974165 30.255803986342993 20.81508627124276 48-49 21.14677562656355 27.738111003296247 30.023283195407892 21.091830174732316 50-51 20.45012512839083 28.04147222448279 30.354072174306236 21.15433047282015 52-53 21.111258779444185 28.37952623504497 29.430555595682822 21.07865938982803 54-55 20.250152999144362 27.899195352100264 29.96390135058159 21.886750298173784 56-57 21.31747832463651 27.597945932967527 29.728189519552544 21.356386222843422 58-59 21.494495384352604 26.57219276247855 30.810707991397585 21.12260386177126 60-61 21.462480561753257 27.20142370065583 30.579525368723015 20.756570368867898 62-63 20.736455475222876 27.553986241109417 30.099703570966035 21.609854712701672 64-65 20.113296416523454 28.02707232724418 30.139614591590913 21.72001666464145 66-67 22.722837370411288 25.78117250757487 30.78402850426396 20.711961617749886 68-69 24.784398532458727 23.99975828265772 31.82377220716167 19.392070977721882 70-71 23.643820934221115 25.02391359385605 31.6068260380864 19.72543943383643 72-73 23.397958552549554 24.92222098958267 31.5718968653758 20.10792359249198 74-75 23.01717093126245 25.986288700656207 30.62478897223976 20.371751395841592 76-77 20.666905620645622 26.725362984405333 30.82306659121435 21.784664803734692 78-79 20.661170613005993 27.453784210903 29.818541250384445 22.06650392570656 80-81 20.474757590055173 28.194996774286736 28.910142220253626 22.420103415404466 82-83 20.50599380567061 28.202975261679846 28.64995298645538 22.641077946194166 84-85 20.041375731020196 28.203140684707208 28.70009239007194 23.05539119420066 86-87 20.29861496618422 28.147958414801426 28.482144103796408 23.07128251521794 88-89 20.25399338103533 28.165601219414732 28.177156002083713 23.403249397466226 90-91 20.617862199492144 27.564010381026925 28.744857019595198 23.07327039988573 92-93 20.696655486454965 27.94652598313997 28.584444360842053 22.772374169563005 94-95 21.10502834845023 26.08028140558341 30.687477978588113 22.127212267378248 96-97 20.44663319780216 26.190321678758426 30.848118071674246 22.514927051765167 98-99 20.577165770353858 26.81874307331991 29.52286059846516 23.081230557861073 100-101 19.509919290038553 27.349334797915674 29.357607883217607 23.783138028828162 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1329.0 1 1022.0 2 757.5 3 882.0 4 973.0 5 1032.5 6 1382.5 7 2042.5 8 2616.5 9 2949.5 10 3018.5 11 2954.5 12 3059.0 13 3229.0 14 3312.0 15 3271.5 16 3178.0 17 3223.5 18 3308.0 19 3402.0 20 3602.0 21 3871.0 22 4219.5 23 4772.5 24 5426.5 25 6260.0 26 7386.0 27 8626.5 28 9804.0 29 11331.0 30 13088.5 31 15019.0 32 17254.0 33 19656.5 34 22132.0 35 24428.5 36 26686.5 37 28630.5 38 30059.0 39 30974.5 40 31655.0 41 32419.5 42 33295.0 43 34545.5 44 36135.5 45 37791.5 46 40007.0 47 42585.5 48 44070.0 49 42712.0 50 39266.5 51 35672.0 52 32762.5 53 30505.5 54 28192.5 55 25633.0 56 22909.5 57 20432.0 58 18041.0 59 15365.5 60 12745.0 61 10287.5 62 7988.0 63 6024.0 64 4531.5 65 3407.0 66 2486.5 67 1799.0 68 1277.0 69 929.0 70 743.0 71 666.0 72 587.0 73 480.5 74 378.5 75 278.5 76 180.0 77 103.0 78 55.0 79 32.0 80 22.0 81 14.5 82 9.0 83 3.5 84 1.0 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020028251389219216 2 0.002792400434073833 3 0.00192579340280954 4 0.0 5 0.05112981484459328 6 0.0 7 0.0 8 1.92579340280954E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.407241753511925E-4 16-17 0.0 18-19 0.0010110415364750086 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.81448350702385E-5 28-29 0.0014924898871773935 30-31 0.007751318446308398 32-33 1.92579340280954E-4 34-35 6.74027690983339E-4 36-37 0.020028251389219216 38-39 0.01882463051246325 40-41 0.020317120399640648 42-43 0.020413410069781125 44-45 0.02084671358541327 46-47 0.022916941493433526 48-49 0.02219476896737995 50-51 0.019209789193025163 52-53 0.016369243923881092 54-55 0.012373222613051295 56-57 0.14140138060129048 58-59 0.7823535698913756 60-61 0.30841581345994784 62-63 4.3330351563214647E-4 64-65 0.15473749991574653 66-67 1.468176745466923 68-69 2.8010183595514055 70-71 2.2551040746899713 72-73 1.9202086019413922 74-75 1.6126593955127086 76-77 1.1396845357826857 78-79 0.4409103995732442 80-81 2.88869010421431E-4 82-83 2.407241753511925E-4 84-85 1.444345052107155E-4 86-87 0.0037071523004083644 88-89 1.92579340280954E-4 90-91 0.2322506843788305 92-93 0.18102457986409676 94-95 1.3443963745013399 96-97 1.3287493031035122 98-99 1.0403135961977135 100-101 0.41751200972910824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1038533.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.095113913710314 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0502853666824 41.971839064553095 2 11.855570623838428 12.589457455694777 3 3.770284983887018 6.005511321198981 4 1.6723750836612377 3.551797822937769 5 0.88158658920379 2.340397018928728 6 0.5459979406655097 1.7393893713771886 7 0.3574803386174835 1.3286321510564916 8 0.2576357477712415 1.0943359500926404 9 0.18797951941177343 0.8982714596951367 >10 1.2439829149154435 12.6535578062019 >50 0.11480478553426914 4.148035071749288 >100 0.05603209587170531 5.380183712152136 >500 0.0023573372490008613 0.8308903574647445 >1k 0.002901338152616445 2.5528456347830475 >5k 7.253345381541112E-4 2.9148558021141526 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9951 0.9581785075678867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7304 0.703299750706044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6523 0.6280975183263314 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6497 0.6255939869026791 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3198 0.30793436510924543 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2560 0.2465015555596211 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2289 0.22040705495155183 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2041 0.19652721675671356 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1800 0.17332140625285858 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1730 0.1665811293430252 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1640 0.15791505903038228 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1479 0.14241242213776548 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1394 0.13422780017582492 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1326 0.1276801026062725 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1215 0.11699194922067954 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1209 0.11641421119983669 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1180 0.11362181076576285 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1166 0.11227375538379618 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1147 0.11044425165112712 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1141 0.10986651363028424 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.81448350702385E-5 0.0 18-19 0.0 0.0 0.0 2.88869010421431E-4 0.0 20-21 0.0 0.0 0.0 4.81448350702385E-4 0.0 22-23 0.0 0.0 0.0 6.258828559131005E-4 0.0 24-25 0.0 0.0 0.0 0.001348055381966678 0.0 26-27 0.0 0.0 0.0 0.002647965928863118 0.0 28-29 0.0 0.0 0.0 0.008136477126870306 0.0 30-31 0.0 0.0 0.0 0.027249976649754988 0.0 32-33 0.0 0.0 0.0 0.0711580662338125 0.0 34-35 0.0 0.0 0.0 0.13856083533214641 0.0 36-37 0.0 0.0 0.0 0.25141232873678543 0.0 38-39 0.0 0.0 0.0 0.4400919373770501 0.0 40-41 0.0 0.0 0.0 0.6555882191514377 0.0 42-43 0.0 0.0 0.0 0.856929919415175 0.0 44-45 0.0 0.0 0.0 1.0938025079607485 0.0 46-47 0.0 0.0 0.0 1.3697205577482854 0.0 48-49 0.0 0.0 0.0 1.6418833104003436 0.0 50-51 0.0 0.0 0.0 1.9383592047628722 0.0 52-53 0.0 0.0 0.0 2.206140777423539 0.0 54-55 0.0 0.0 0.0 2.47883312326137 0.0 56-57 0.0 0.0 0.0 2.718353677735806 0.0 58-59 0.0 0.0 0.0 2.872031991280007 0.0 60-61 0.0 0.0 0.0 2.986664843582245 0.0 62-63 0.0 0.0 0.0 3.0813657341654044 0.0 64-65 0.0 0.0 0.0 3.174766714201667 0.0 66-67 0.0 9.6289670140477E-5 0.0 3.250209670756731 0.0 68-69 0.0 9.6289670140477E-5 0.0 3.352228576270566 0.0 70-71 0.0 9.6289670140477E-5 0.0 3.538260218981968 0.0 72-73 0.0 9.6289670140477E-5 0.0 3.7808620429008997 0.0 74-75 0.0 9.6289670140477E-5 0.0 4.0536506784088715 4.81448350702385E-5 76-77 0.0 9.6289670140477E-5 0.0 4.445742215220894 9.6289670140477E-5 78-79 0.0 9.6289670140477E-5 0.0 5.031038975169783 9.6289670140477E-5 80-81 0.0 9.6289670140477E-5 0.0 5.702659424399609 9.6289670140477E-5 82-83 0.0 9.6289670140477E-5 0.0 6.287185866987375 9.6289670140477E-5 84-85 0.0 9.6289670140477E-5 0.0 6.746054290041819 9.6289670140477E-5 86-87 0.0 1.92579340280954E-4 0.0 7.141612254978899 9.6289670140477E-5 88-89 0.0 1.92579340280954E-4 0.0 7.546028869568902 9.6289670140477E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7350 0.0 25.292267 1 TACATGG 7470 0.0 24.757256 2 CTAGACG 100 0.0017203649 23.588863 4 ACATGGG 7625 0.0 23.14029 3 CGCATAG 225 0.0 22.699139 66-67 CGGCTAA 105 0.002291736 22.453688 9 CATGGGA 5130 0.0 21.61163 4 AGTACTT 6910 0.0 21.358624 12-13 GTAAGGT 310 1.2732926E-11 21.306072 4 TACCTGG 610 0.0 20.881947 2 GAGTACT 6795 0.0 20.540415 12-13 GTACTTT 7125 0.0 19.390532 14-15 TAAGGTG 390 0.0 19.354967 5 CATGGGG 2175 0.0 19.30491 4 GTATCAA 14765 0.0 19.045471 1 AACGCGT 90 1.05430445E-4 18.85508 62-63 AGTACAT 5730 0.0 18.854626 2 TACTTTT 7920 0.0 18.783699 14-15 ATGGGAT 2110 0.0 18.558064 5 AGGACCG 205 1.6851069E-5 18.41082 5 >>END_MODULE