FastQCFastQC Report
Fri 27 May 2016
SRR939718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129620
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102760.9096864432286964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64270.5689523910695632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54540.4828172305731131No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54240.4801614702289265No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32000.2832811033798977No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28960.2563693985588074No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28880.2556611958003577No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27280.24149714063136274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21540.1906835927125936No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15630.13836511393211876No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15600.1380995378977001No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15090.133584745312583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.12083709566048759No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13220.11703050583382023No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC13120.11614525238575804No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.10437138152653105No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11680.10339760273366265No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11650.103132026699244No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG753.255966E-431.3653184
GTACATG80900.027.5621451
TACATGG82350.026.6781522
TCTCGCG550.001034579925.74148894-95
ACATGGG82950.025.6911933
CATGGGA58000.024.091814
GTGTAAG4900.024.000881
ATGGGAT22650.023.6818775
GTCTAGC2853.6379788E-1223.1082151
CATGGGG20950.022.457274
GAGTACT68950.022.34743312-13
AGTACTT69950.022.29699912-13
GTATCAA147300.021.8761581
GTATAGG3301.8189894E-1221.3826031
GTACTTT73400.021.06181514-15
CCTATAC3052.3646862E-1020.0514583
GTCGGGA3553.6379788E-1219.8776662
AGTACAT60950.019.3732512
GAGTACG1708.56864E-519.3701211
ACTTTTT82300.019.21729316-17
Produced by FastQC (version 0.11.5)