FastQCFastQC Report
Fri 27 May 2016
SRR939718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129620
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110690.9798870416600273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79170.700855154830828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71200.6303004550202723No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70480.6239266301942246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33930.30036649492749773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27140.2402577858040757No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23040.20396239443352632No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22040.19510985995290453No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20120.17811299375011064No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.17528018271631168No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17930.1587259432375489No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16280.14411926134452294No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15300.13544377755351356No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13850.12260760255661195No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.12216497583258086No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13120.11614525238575804No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.11402064411040881No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12510.11074520635257874No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12450.11021405428374142No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12270.1086205980772295No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGCG250.001553105238.3089964-65
ACGCATA1400.029.07378664-65
CGCATAG1400.029.07378666-67
TCGACGA350.00840971527.13195640-41
ACGTGCC909.0289535E-426.3479338
GTACATG77850.024.740121
TACATGG79200.023.9547862
CGATCGT601.7296466E-423.71313924-25
GTATAGG2755.2750693E-1122.4256521
ACATGGG80850.022.2906423
AGTACTT81200.021.639712-13
GTCGGGA4050.021.0802142
CGGGAGT4150.020.5704354
GAGTACT79400.020.3980812-13
CATGGGG22650.020.1012044
AGTACAT61800.019.7243232
CATGGGA55750.019.6510684
AACGCAT2200.019.58982564-65
GTACTTT84450.019.29061914-15
GTATCAA163500.019.033581
Produced by FastQC (version 0.11.5)