##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939718_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1129620 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.977462332465784 31.0 31.0 33.0 28.0 34.0 2 31.194346771480674 31.0 31.0 34.0 28.0 34.0 3 31.21622315468919 31.0 31.0 34.0 28.0 34.0 4 35.06952337954356 35.0 35.0 37.0 32.0 37.0 5 34.7986092668331 35.0 35.0 37.0 32.0 37.0 6 34.87287406384448 35.0 35.0 37.0 32.0 37.0 7 34.76512809617393 35.0 35.0 37.0 32.0 37.0 8 34.73575361626033 35.0 35.0 37.0 31.0 37.0 9 36.20957313078735 38.0 35.0 39.0 32.0 39.0 10-11 36.06542421345231 37.0 35.0 39.0 30.5 39.0 12-13 35.96644579593138 37.0 35.0 39.0 30.0 39.0 14-15 36.76196242984366 38.0 36.0 40.0 31.0 41.0 16-17 36.70711256882846 38.0 36.0 40.0 31.0 41.0 18-19 36.75947309714771 38.5 35.5 40.0 31.0 41.0 20-21 36.69761158619713 38.0 35.0 40.0 30.0 41.0 22-23 36.53119411837609 38.0 35.0 40.0 30.0 41.0 24-25 36.320820718471694 38.0 34.5 40.0 30.0 41.0 26-27 35.91608903879181 38.0 34.0 40.0 29.0 41.0 28-29 35.63997229156708 38.0 34.0 40.0 27.5 41.0 30-31 35.268823586692875 37.5 34.0 40.0 27.0 41.0 32-33 35.12752961172784 37.0 33.5 40.0 27.0 41.0 34-35 34.85723827481808 37.0 33.0 40.0 26.5 41.0 36-37 34.77535498663268 37.0 33.0 40.0 25.5 41.0 38-39 34.582445866751655 37.0 33.0 40.0 25.0 41.0 40-41 34.43826508029249 37.0 33.0 40.0 25.0 41.0 42-43 34.088041553796856 37.0 33.0 40.0 24.0 41.0 44-45 33.69255723163542 37.0 33.0 40.0 23.5 41.0 46-47 33.21156273791186 37.0 32.0 40.0 21.0 41.0 48-49 32.74930463341654 36.5 31.0 39.5 18.5 41.0 50-51 32.44690736707919 36.0 31.0 39.0 17.0 40.0 52-53 32.15026955967494 36.0 31.0 39.0 15.0 40.0 54-55 31.836008126626652 35.5 30.0 39.0 13.0 40.0 56-57 31.403363520475914 35.0 29.5 39.0 8.5 40.0 58-59 30.97293824471946 35.0 29.0 38.0 8.0 40.0 60-61 30.560555319487968 35.0 28.0 38.0 7.5 40.0 62-63 30.248106000247873 34.0 28.0 38.0 6.0 40.0 64-65 29.753528620243976 34.0 28.0 38.0 2.0 40.0 66-67 28.745583027920894 33.5 25.5 37.0 2.0 39.0 68-69 26.727831483153626 32.0 21.0 36.0 2.0 39.0 70-71 26.01735008232857 31.0 19.0 35.0 2.0 38.0 72-73 26.49869336591066 31.0 21.5 35.0 2.0 38.0 74-75 26.60944831005117 31.0 22.5 35.0 2.0 37.5 76-77 26.39468449567111 31.0 23.5 34.0 2.0 36.0 78-79 26.93033852091854 32.0 25.0 35.0 2.0 37.0 80-81 26.69035295054974 32.0 24.5 35.0 2.0 36.0 82-83 26.16723367150015 32.0 23.5 34.5 2.0 36.0 84-85 25.71261928790213 31.0 21.5 34.0 2.0 35.5 86-87 25.243305713425755 31.0 20.0 34.0 2.0 35.0 88-89 24.646428444963792 31.0 18.5 34.0 2.0 35.0 90-91 23.981386661001046 31.0 14.5 34.0 2.0 35.0 92-93 23.329018165400754 30.0 6.0 34.0 2.0 35.0 94-95 22.342420902604417 29.0 2.0 34.0 2.0 35.0 96-97 21.596021671004408 29.0 2.0 34.0 2.0 35.0 98-99 20.75251987393991 29.0 2.0 34.0 2.0 35.0 100-101 19.258135479187693 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.207605978869708 1101 2 0.18449663195083943 1101 3 0.2835131805305444 1101 4 0.19795640476147014 1101 5 0.20745990720430285 1101 6 -0.13681664890766854 1101 7 -0.08455086043955617 1101 8 -0.0358249365070904 1101 9 0.11309879844178283 1101 10-11 0.1569631870303283 1101 12-13 0.263829803446626 1101 14-15 0.24399413066992537 1101 16-17 0.3767511183439609 1101 18-19 0.17456424890324485 1101 20-21 0.20377209534201057 1101 22-23 0.15900805957848974 1101 24-25 0.16808754170883589 1101 26-27 0.2620888813572648 1101 28-29 0.20073266739471762 1101 30-31 0.02602729121716152 1101 32-33 -0.021513294956285733 1101 34-35 -0.17749401011131738 1101 36-37 0.10301645271373872 1101 38-39 0.021702898164569717 1101 40-41 -0.11064418234271045 1101 42-43 -0.23583773526327434 1101 44-45 -0.25643218869180373 1101 46-47 -0.20878761564103598 1101 48-49 -0.23756234448470082 1101 50-51 -0.36706302520914846 1101 52-53 -0.418338342935094 1101 54-55 -0.5428245097909823 1101 56-57 -0.6484714968983276 1101 58-59 -0.46562215869406387 1101 60-61 -0.6474081453717098 1101 62-63 -0.5382854352750677 1101 64-65 -0.5519437594090881 1101 66-67 -0.6330566898431975 1101 68-69 -8.808651860327544 1101 70-71 -7.2945903277270645 1101 72-73 -2.797752924126865 1101 74-75 -1.2625471667779884 1101 76-77 -0.9902143019932588 1101 78-79 -1.2964254270738742 1101 80-81 -1.3429132522010185 1101 82-83 -1.1448755444497785 1101 84-85 -1.0398226746393924 1101 86-87 -0.9507510469020239 1101 88-89 -0.9266068215558398 1101 90-91 -1.509357597815292 1101 92-93 -1.4126531176594739 1101 94-95 -0.985849466065936 1101 96-97 -1.507282435117311 1101 98-99 -1.3103621491867088 1101 100-101 -1.1014248044756307 1102 1 0.24991049597011283 1102 2 0.11553004322568938 1102 3 0.17689102362446008 1102 4 0.23317697736678156 1102 5 0.1484981277366515 1102 6 0.24518034407053335 1102 7 0.3250248585303197 1102 8 0.33078313866703724 1102 9 0.43968269314940756 1102 10-11 0.3988307043447108 1102 12-13 0.17063790418539782 1102 14-15 0.3261332121370586 1102 16-17 0.21071732924932718 1102 18-19 0.2780038920153842 1102 20-21 0.2738022291184663 1102 22-23 0.30751383527783105 1102 24-25 0.26712576082878314 1102 26-27 0.20594874481954406 1102 28-29 0.17619450794911273 1102 30-31 0.06384281487891741 1102 32-33 -0.1535450685412414 1102 34-35 -0.012680190522743828 1102 36-37 0.1769522544226092 1102 38-39 0.2706148124821013 1102 40-41 -0.048536258649598096 1102 42-43 0.03255626227489472 1102 44-45 -0.0040573971106212525 1102 46-47 0.14180301908914572 1102 48-49 -0.07104657033195849 1102 50-51 0.07914355817708696 1102 52-53 0.23289974073468755 1102 54-55 0.0959109167816834 1102 56-57 -0.14729107488421533 1102 58-59 -0.37764876285685745 1102 60-61 -0.09236121450426182 1102 62-63 -0.0821909195941366 1102 64-65 -0.04257065071492505 1102 66-67 0.1074513824354959 1102 68-69 -3.812707596867803 1102 70-71 0.32875900586238416 1102 72-73 0.6594249103513441 1102 74-75 0.4288746997365145 1102 76-77 -0.06982234397574771 1102 78-79 0.09315126645919136 1102 80-81 0.1899528322229891 1102 82-83 0.2164660053458114 1102 84-85 0.09661899740031998 1102 86-87 0.2103099981828258 1102 88-89 -0.1300913793195413 1102 90-91 -0.33055138123203776 1102 92-93 -0.2928824789886626 1102 94-95 0.07936081712941956 1102 96-97 -0.48633991951547273 1102 98-99 -0.8285239817609913 1102 100-101 -0.8383926700198039 1103 1 0.2647051070950006 1103 2 0.10598004284999973 1103 3 0.2019043605644022 1103 4 0.2900111259440479 1103 5 0.23831772214339964 1103 6 0.05822274426890317 1103 7 0.20743437396735942 1103 8 0.1929731017744345 1103 9 0.19740872610479698 1103 10-11 0.3224908114910576 1103 12-13 0.23072927161621237 1103 14-15 0.2243103717821029 1103 16-17 0.12213374984005299 1103 18-19 0.35465184939727834 1103 20-21 0.2908885397827632 1103 22-23 0.19747093165694451 1103 24-25 0.21653931115975666 1103 26-27 0.11978835590686998 1103 28-29 -0.06352477764172448 1103 30-31 0.04120758582167383 1103 32-33 0.06406119493657769 1103 34-35 0.010124098336596887 1103 36-37 -0.18832211085175032 1103 38-39 0.03893901658612009 1103 40-41 0.13153872876484485 1103 42-43 0.24142025554256463 1103 44-45 0.10756339799641523 1103 46-47 0.26360872805081925 1103 48-49 0.02479530991359269 1103 50-51 0.00826993225759054 1103 52-53 0.08236137918715514 1103 54-55 0.19112539731726486 1103 56-57 0.1487626936466171 1103 58-59 0.22648831834444394 1103 60-61 -0.19013037649360598 1103 62-63 -0.3753819421429547 1103 64-65 -0.2786006757357775 1103 66-67 0.034327382705988896 1103 68-69 -4.094233493072597 1103 70-71 0.07963999527548538 1103 72-73 0.029312729780450297 1103 74-75 -0.05403898959919573 1103 76-77 -0.5065488953811972 1103 78-79 -0.37604603760049216 1103 80-81 -0.2060829032622884 1103 82-83 -0.4950465914878883 1103 84-85 -0.48295932988443013 1103 86-87 -0.8069942117819373 1103 88-89 -0.9911768183866307 1103 90-91 -1.1884209271749775 1103 92-93 -1.0257116384233278 1103 94-95 -0.7754806799139118 1103 96-97 -1.284677873816726 1103 98-99 -0.7926645192960216 1103 100-101 -0.9134176533016678 1104 1 0.1687687884765623 1104 2 0.2150956304424092 1104 3 0.2725364013951186 1104 4 0.09021153707161744 1104 5 0.1498913969621114 1104 6 0.20301910595267714 1104 7 0.07035139996385453 1104 8 0.10104975157293694 1104 9 0.2495732476401784 1104 10-11 0.08618398991933418 1104 12-13 0.04168110243558942 1104 14-15 0.2501838144601791 1104 16-17 0.3497546202589419 1104 18-19 -0.007731944691805381 1104 20-21 0.035683681101083664 1104 22-23 0.18267332248847623 1104 24-25 0.008004956516138861 1104 26-27 0.09179249430173542 1104 28-29 -0.12367286221034846 1104 30-31 0.2216077707748454 1104 32-33 0.06647582359293125 1104 34-35 0.035393121122176296 1104 36-37 0.08705911775795272 1104 38-39 0.17540163814808096 1104 40-41 0.005416331533176333 1104 42-43 -0.01422630763711652 1104 44-45 -0.17766889178885492 1104 46-47 -0.21496538338752913 1104 48-49 -0.02793600357931325 1104 50-51 -0.03303774356994893 1104 52-53 -0.5603071397230899 1104 54-55 -0.6239373701461659 1104 56-57 -0.5542057775337774 1104 58-59 -0.6409366020606129 1104 60-61 -0.6927294159303905 1104 62-63 -0.6276596186755974 1104 64-65 -0.49101036869182835 1104 66-67 -0.517945954749468 1104 68-69 -5.1156007362941445 1104 70-71 -0.5111931617828382 1104 72-73 -0.16159039489750882 1104 74-75 -0.31985492594493437 1104 76-77 -0.09280127509384428 1104 78-79 -0.5082187355844532 1104 80-81 -0.6284185064753771 1104 82-83 -0.5156348347582487 1104 84-85 -0.6467015263253018 1104 86-87 -0.46323590651700997 1104 88-89 -0.6447086238224067 1104 90-91 -0.7493551304932886 1104 92-93 -1.0375227421218476 1104 94-95 -0.4409151164576208 1104 96-97 -0.9922450888342738 1104 98-99 -0.8951419967734999 1104 100-101 -0.940983336082585 1105 1 0.14993497739949646 1105 2 0.1452818955846915 1105 3 0.1207621600192148 1105 4 0.05896325310513362 1105 5 0.19951908632984328 1105 6 0.12517707987995408 1105 7 -0.007895610019346577 1105 8 0.1820245684378392 1105 9 0.3481435857948796 1105 10-11 0.11877352568421884 1105 12-13 -0.0014146769456502284 1105 14-15 -0.055360072067443866 1105 16-17 0.03886357328629941 1105 18-19 0.054428967084852786 1105 20-21 -0.055157642084317615 1105 22-23 -0.009802488303044754 1105 24-25 -0.0072546999476870155 1105 26-27 0.145666337008727 1105 28-29 0.06414563361077086 1105 30-31 0.18136266627526254 1105 32-33 0.23801533903998262 1105 34-35 0.1802908729809829 1105 36-37 0.08724925427184616 1105 38-39 0.4328478507934648 1105 40-41 0.3270140548371856 1105 42-43 0.2172261156767803 1105 44-45 -0.08684335611183513 1105 46-47 0.06152536925105068 1105 48-49 0.1842549731046006 1105 50-51 0.08701115652120706 1105 52-53 0.106461802541304 1105 54-55 0.22129905808793637 1105 56-57 0.19301012968509568 1105 58-59 0.19413473002070347 1105 60-61 0.2058962471449597 1105 62-63 0.37211317848649017 1105 64-65 0.33028670553966677 1105 66-67 0.8310684928799326 1105 68-69 1.4705276724348373 1105 70-71 1.879142686569221 1105 72-73 0.897493325572988 1105 74-75 0.5956558205433744 1105 76-77 0.16015648027940088 1105 78-79 0.18024498883978524 1105 80-81 0.34591855545559014 1105 82-83 0.1272228964532438 1105 84-85 -0.09349693669069126 1105 86-87 -0.14103551224114597 1105 88-89 -0.0990985121292205 1105 90-91 -0.29622343796256345 1105 92-93 -0.26544356845376527 1105 94-95 0.10705076605036723 1105 96-97 -0.5463578034071048 1105 98-99 -0.38747201335725023 1105 100-101 -0.35460850494028406 1106 1 0.44739309623460244 1106 2 0.37770744360965836 1106 3 0.35733364502594256 1106 4 0.33656614024100406 1106 5 0.5039646006935072 1106 6 0.579312523423198 1106 7 0.47745061172552994 1106 8 0.5023501530869723 1106 9 0.7206750558227384 1106 10-11 0.5256782082268359 1106 12-13 0.6020506268085342 1106 14-15 0.7629985072444327 1106 16-17 0.7223495758623315 1106 18-19 0.6289653011300871 1106 20-21 0.6202803046584009 1106 22-23 0.6299958319237788 1106 24-25 0.9329028662329932 1106 26-27 0.7386196844156174 1106 28-29 0.7332299062097292 1106 30-31 0.6976357261207866 1106 32-33 0.921572326641261 1106 34-35 0.9544391555548799 1106 36-37 1.0689742109606968 1106 38-39 1.1805794162910317 1106 40-41 0.9372414538224021 1106 42-43 0.4997804655156699 1106 44-45 0.9054429378114364 1106 46-47 0.6295557714172233 1106 48-49 0.8697470742202 1106 50-51 1.0555565601893804 1106 52-53 0.6021728667405739 1106 54-55 0.6306717562184616 1106 56-57 0.6585144937209968 1106 58-59 0.9187838170915512 1106 60-61 1.0224308199385597 1106 62-63 0.8805182169830097 1106 64-65 0.726324506608762 1106 66-67 1.205622521743063 1106 68-69 2.397893531312487 1106 70-71 2.7918485945808555 1106 72-73 1.734706144851284 1106 74-75 1.3792327662532067 1106 76-77 0.56081036552645 1106 78-79 0.5038626326307565 1106 80-81 0.8649435709899933 1106 82-83 0.8283327318914395 1106 84-85 0.8461997412101283 1106 86-87 0.8148560391005724 1106 88-89 1.2291516761354124 1106 90-91 1.01512922798808 1106 92-93 1.094558530233936 1106 94-95 1.2837139760549334 1106 96-97 1.2744050078467986 1106 98-99 1.4109994424324555 1106 100-101 1.5024955529341177 1107 1 0.09922325236674467 1107 2 0.0583323643809841 1107 3 0.08452926859150978 1107 4 0.21489120577756182 1107 5 0.11262502151420506 1107 6 0.11381840613044147 1107 7 0.17963203541142292 1107 8 0.003971845880712976 1107 9 0.06822157574914911 1107 10-11 0.14172186611584436 1107 12-13 0.03902031995805544 1107 14-15 0.1699043762245438 1107 16-17 0.060493709368522275 1107 18-19 -0.015952262527740402 1107 20-21 0.008175143606194979 1107 22-23 0.04046445246587638 1107 24-25 -0.10540949470098582 1107 26-27 0.11517948254270038 1107 28-29 -0.0363545468305162 1107 30-31 0.05227623279210292 1107 32-33 0.06316071012415847 1107 34-35 0.1391867688895303 1107 36-37 0.027656588192144227 1107 38-39 0.29096623432037205 1107 40-41 0.22424150775042762 1107 42-43 0.3780488015699177 1107 44-45 0.23806400097796399 1107 46-47 0.33862982720400936 1107 48-49 0.10790759624349278 1107 50-51 0.33107414667907875 1107 52-53 0.3513483632461103 1107 54-55 0.3699612649761619 1107 56-57 0.23764196626848033 1107 58-59 0.21388673207646747 1107 60-61 0.21965366665199682 1107 62-63 0.3021040651483027 1107 64-65 -0.10867534281298319 1107 66-67 0.5676207021974484 1107 68-69 1.7722118145242725 1107 70-71 2.10888130648123 1107 72-73 1.2721031300644974 1107 74-75 0.48681777818390515 1107 76-77 0.15627172679590373 1107 78-79 0.3373595009532231 1107 80-81 0.2845211254450426 1107 82-83 0.5583420677432791 1107 84-85 0.4661045057162383 1107 86-87 0.4078433646136439 1107 88-89 0.1449427127371372 1107 90-91 0.36744172479266624 1107 92-93 0.26092859275543034 1107 94-95 0.45784232055557084 1107 96-97 -0.07459826915626167 1107 98-99 -0.06904123470745205 1107 100-101 -0.08660685358654874 1108 1 -0.11002171581835185 1108 2 -0.14836214672535064 1108 3 -0.24645615256294917 1108 4 -0.15688324841146084 1108 5 -0.24019941958617608 1108 6 -0.0853548889460356 1108 7 -0.2061143608248983 1108 8 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-0.723330882272176 2316 84-85 -0.9101206632957641 2316 86-87 -0.8552323850201091 2316 88-89 -1.0017726414824537 2316 90-91 -0.7382617582658106 2316 92-93 -1.081423194134885 2316 94-95 -0.9843819513151857 2316 96-97 -0.844421058559913 2316 98-99 -0.737403901535405 2316 100-101 -0.64653429266831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 18.0 4 60.0 5 125.0 6 274.0 7 703.0 8 1387.0 9 2216.0 10 3538.0 11 5195.0 12 7077.0 13 9035.0 14 11283.0 15 14024.0 16 16834.0 17 19258.0 18 16625.0 19 13658.0 20 13390.0 21 13681.0 22 14777.0 23 16570.0 24 19494.0 25 23266.0 26 28160.0 27 33961.0 28 40052.0 29 47111.0 30 55215.0 31 63764.0 32 74145.0 33 86680.0 34 102728.0 35 119408.0 36 121796.0 37 98721.0 38 34149.0 39 1234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57521476651502 21.543326078525475 15.161869579923604 20.719589575035904 2 16.40842476572295 23.581499373136232 37.36364827630172 22.646427584839106 3 21.30442095571962 26.87709141127105 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20.923806235725287 26.642499247534566 31.602751367716575 20.830943149023565 24-25 21.161515519834566 26.891367797460912 31.757787360163032 20.189329322541493 26-27 20.975649268734447 26.62057139720808 32.24575232136202 20.158027012695452 28-29 20.183840746304725 27.62714685216874 32.07311491750758 20.115897484018948 30-31 20.41398157160556 27.058012217431504 31.80502569237055 20.722980518592387 32-33 20.318882931516878 27.22199328168966 31.679413809938833 20.77970997685463 34-35 21.248305232168256 26.929932775869254 31.61344553437806 20.208316457584438 36-37 21.215592875534504 26.46781284721668 31.834686315476095 20.481907961772716 38-39 20.59585985709286 27.09370473241458 32.0355185810287 20.274916829463855 40-41 20.498481315035868 27.23585159772038 31.77555891859707 20.490108168646685 42-43 20.286771856219776 27.447562129102714 31.131426394263677 21.134239620413833 44-45 20.76757425819333 27.04256824435286 30.915968817517264 21.273888679936544 46-47 21.16993062474058 27.725163936205416 30.30247187845664 20.80243356059737 48-49 21.128286683160532 27.71394588033605 30.095441447148385 21.06232598935503 50-51 20.434726946771463 27.974096057573767 30.38862558401926 21.20255141163551 52-53 21.11082223894433 28.440316180674923 29.4266645348196 21.022197045561146 54-55 20.329048615493626 27.83582998416758 30.00507080302477 21.830050597314024 56-57 20.98491684473108 27.89670876749909 29.525101547632858 21.593272840136972 58-59 21.487531597097714 27.426679239102047 29.413920873341564 21.671868290458672 60-61 20.71951394960215 27.88738504443289 30.211396509556117 21.181704496408848 62-63 20.701893233191868 27.635530036242855 30.071520765721022 21.591055964844255 64-65 20.342521236051045 28.166152456919257 29.6876673763794 21.803658930650293 66-67 20.5803645056545 27.8492410713494 29.337393839545868 22.23300058345023 68-69 20.804811191497194 27.376099282393433 29.768491639185534 22.050597886923843 70-71 21.04923949471513 27.58718475255798 29.611568925514042 21.752006827212846 72-73 20.880623359114452 27.35750904704463 29.652930532888668 22.108937060952247 74-75 20.195371667079172 28.622307861577717 28.895976962383667 22.28634350895944 76-77 20.398404257674308 27.91296031454366 29.01315469048178 22.675480737300255 78-79 20.54282558800941 27.964437243952805 28.944028719337965 22.548708448699816 80-81 20.482426340239154 28.18389654767938 28.83368264500123 22.499994467080235 82-83 20.499902622120715 28.148005523981517 28.740948283493562 22.611143570404206 84-85 20.018917924800142 28.15428953100455 28.77175572417719 23.05503682001812 86-87 20.301157569705598 28.064912783903793 28.574160239658358 23.059769406732247 88-89 20.28281216941287 28.13745165405327 28.24806417030875 23.33167200622511 90-91 20.325607376692172 27.82543917734585 28.488808546651416 23.36014489931056 92-93 20.53722004297123 28.151643999210336 28.373759398296556 22.93737655952188 94-95 20.226769323457493 27.600604245667505 28.759834496934474 23.412791933940532 96-97 19.893626299290474 27.521251055303438 28.914539347600016 23.670583297806076 98-99 20.221071472368056 27.81362987794053 28.05229463033083 23.913004019360574 100-101 19.9012485148322 27.52682155498618 28.473123553566086 24.098806376615535 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2455.0 1 1638.0 2 1154.5 3 1597.5 4 1432.0 5 1249.5 6 2128.0 7 4208.5 8 5843.0 9 5732.0 10 4387.5 11 3150.0 12 2718.0 13 2620.5 14 2657.5 15 2672.0 16 2703.0 17 2855.0 18 2968.5 19 3167.5 20 3496.0 21 3834.5 22 4332.5 23 4886.5 24 5594.5 25 6534.0 26 7955.0 27 9569.5 28 10776.0 29 12375.5 30 14279.5 31 16123.0 32 18779.5 33 21494.0 34 23873.5 35 26030.5 36 28764.0 37 31311.0 38 32081.5 39 32130.5 40 32003.5 41 32723.5 42 33861.0 43 34747.5 44 35853.0 45 37177.5 46 38951.5 47 43593.5 48 52201.5 49 51735.0 50 42884.5 51 37231.5 52 33837.0 53 32464.5 54 31108.5 55 28466.0 56 25678.5 57 23292.5 58 21132.5 59 18717.5 60 15883.0 61 13051.5 62 10397.5 63 7890.5 64 5856.0 65 4547.0 66 3416.0 67 2442.5 68 1728.0 69 1194.5 70 947.5 71 834.5 72 767.0 73 693.5 74 585.0 75 486.0 76 378.0 77 226.0 78 130.0 79 84.0 80 36.5 81 17.0 82 15.5 83 9.0 84 3.0 85 4.0 86 3.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022485437580779376 2 0.016642764823568988 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.852534480621803E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.852534480621803E-5 26-27 2.655760344186541E-4 28-29 8.852534480621803E-5 30-31 3.983640516279811E-4 32-33 0.0018147695685274694 34-35 0.0035852764646518297 36-37 0.08870239549583045 38-39 0.08100069049768949 40-41 0.08958764894389264 42-43 0.09051716506435792 44-45 0.08617942316885324 46-47 0.07998264903241799 48-49 0.08122200385970503 50-51 0.08069085179086773 52-53 0.06272020679520547 54-55 0.05377914696977745 56-57 0.030718294647757653 58-59 0.014606681893025974 60-61 0.009516474566668438 62-63 0.0015934562065119244 64-65 0.004868893964341991 66-67 0.16341778651227848 68-69 0.4345709176537243 70-71 0.41695437403728686 72-73 0.1929852516775553 74-75 0.029788778527292364 76-77 0.03231175085426958 78-79 0.005975460774419716 80-81 0.0015491935341088153 82-83 0.0 84-85 3.0983870682176306E-4 86-87 0.0010623041376746163 88-89 8.852534480621803E-5 90-91 7.082027584497442E-4 92-93 0.0023901843097678867 94-95 0.08321382411784493 96-97 0.0181919583576778 98-99 0.018723110426515113 100-101 0.010445990687133725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1129620.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.1777061361042 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4368045953147 40.92652540429357 2 12.137748506282154 12.666397494294557 3 3.9158554001917754 6.129610570280495 4 1.7307465713726398 3.61225543988606 5 0.9361866669832212 2.4424036399194677 6 0.5676438230132584 1.7771011552296323 7 0.3623041389334848 1.3232939225215945 8 0.2648572519152256 1.105571508675901 9 0.20057860283016515 0.9419158256096445 >10 1.2645047102795646 12.741881704438143 >50 0.11687827992257659 4.19011869794232 >100 0.05977781407022442 5.664122914742976 >500 0.002377526235407521 0.8059070745540948 >1k 0.003056819445523955 2.7351218988742434 >5k 5.094699075873258E-4 1.9569497241921037 >10k+ 1.6982330252910863E-4 0.9808230245452749 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11069 0.9798870416600273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7917 0.700855154830828 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7120 0.6303004550202723 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7048 0.6239266301942246 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3393 0.30036649492749773 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2714 0.2402577858040757 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2304 0.20396239443352632 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2204 0.19510985995290453 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2012 0.17811299375011064 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1980 0.17528018271631168 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1793 0.1587259432375489 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1628 0.14411926134452294 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1530 0.13544377755351356 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1385 0.12260760255661195 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1380 0.12216497583258086 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1312 0.11614525238575804 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.11402064411040881 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1251 0.11074520635257874 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1245 0.11021405428374142 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1227 0.1086205980772295 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.852534480621803E-5 0.0 16-17 0.0 0.0 0.0 8.852534480621803E-5 0.0 18-19 0.0 0.0 0.0 1.3278801720932704E-4 0.0 20-21 0.0 0.0 0.0 1.7705068961243605E-4 0.0 22-23 0.0 0.0 0.0 3.983640516279811E-4 0.0 24-25 0.0 0.0 0.0 9.295161204652892E-4 0.0 26-27 0.0 0.0 0.0 0.0023459216373647772 0.0 28-29 0.0 0.0 0.0 0.008188594394575166 0.0 30-31 0.0 0.0 0.0 0.027044492838299603 0.0 32-33 0.0 0.0 0.0 0.06873993024202829 0.0 34-35 0.0 0.0 0.0 0.1350454135018856 0.0 36-37 0.0 0.0 0.0 0.2440643756307431 0.0 38-39 0.0 0.0 0.0 0.4322692586887626 0.0 40-41 0.0 0.0 0.0 0.6556629663072537 0.0 42-43 0.0 0.0 0.0 0.8666631256528744 0.0 44-45 0.0 0.0 0.0 1.0989093677519874 0.0 46-47 0.0 0.0 0.0 1.3875462544926611 0.0 48-49 0.0 0.0 0.0 1.7092473575184575 0.0 50-51 0.0 0.0 0.0 2.062950372691702 0.0 52-53 0.0 0.0 0.0 2.4028876967475785 0.0 54-55 0.0 0.0 0.0 2.730077371151361 0.0 56-57 0.0 0.0 0.0 3.1132593261450756 0.0 58-59 0.0 0.0 0.0 3.508569253377242 0.0 60-61 0.0 0.0 0.0 3.9216727749154585 0.0 62-63 0.0 0.0 0.0 4.3227368495600285 0.0 64-65 0.0 0.0 0.0 4.700076131796533 0.0 66-67 0.0 0.0 0.0 5.110302579628548 0.0 68-69 0.0 0.0 0.0 5.581877091411271 0.0 70-71 0.0 0.0 0.0 6.124758768435402 0.0 72-73 0.0 0.0 0.0 6.674589685026824 4.426267240310901E-5 74-75 0.0 0.0 0.0 7.229466546272198 8.852534480621803E-5 76-77 0.0 0.0 0.0 7.7857155503620685 8.852534480621803E-5 78-79 0.0 0.0 0.0 8.430534161930561 8.852534480621803E-5 80-81 0.0 0.0 0.0 9.152723924859687 8.852534480621803E-5 82-83 0.0 0.0 0.0 9.829367397886015 8.852534480621803E-5 84-85 0.0 0.0 0.0 10.457100617906907 8.852534480621803E-5 86-87 0.0 0.0 0.0 11.0791682158602 8.852534480621803E-5 88-89 0.0 0.0 0.0 11.742134523113968 8.852534480621803E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCGCG 25 0.0015531052 38.30899 64-65 ACGCATA 140 0.0 29.073786 64-65 CGCATAG 140 0.0 29.073786 66-67 TCGACGA 35 0.008409715 27.131956 40-41 ACGTGCC 90 9.0289535E-4 26.347933 8 GTACATG 7785 0.0 24.74012 1 TACATGG 7920 0.0 23.954786 2 CGATCGT 60 1.7296466E-4 23.713139 24-25 GTATAGG 275 5.2750693E-11 22.425652 1 ACATGGG 8085 0.0 22.290642 3 AGTACTT 8120 0.0 21.6397 12-13 GTCGGGA 405 0.0 21.080214 2 CGGGAGT 415 0.0 20.570435 4 GAGTACT 7940 0.0 20.39808 12-13 CATGGGG 2265 0.0 20.101204 4 AGTACAT 6180 0.0 19.724323 2 CATGGGA 5575 0.0 19.651068 4 AACGCAT 220 0.0 19.589825 64-65 GTACTTT 8445 0.0 19.290619 14-15 GTATCAA 16350 0.0 19.03358 1 >>END_MODULE