FastQCFastQC Report
Fri 27 May 2016
SRR939716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290116
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.6697321071571372No Hit
NAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18920.652152931930676No Hit
NAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18390.6338843772835693No Hit
NTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.5745977471080533No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14810.5104854609880186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.5080726330157592No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14480.4991107005473673No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.48945938865832983No Hit
NGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.4367218629789464No Hit
NCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10790.37192019743826604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.34710253829502685No Hit
NATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.3360724675646983No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9680.3336596395924389No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.27713052709950503No Hit
NCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7210.24852128114271535No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6870.23680183099174124No Hit
NTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6120.21095010271753367No Hit
NTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5400.18613244357429443No Hit
NCGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5070.1747576831336431No Hit
NATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4980.1716554757407382No Hit
NCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4760.1640723021136373No Hit
NGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4730.163038232982669No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4590.15821257703815025No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4570.15752319761750472No Hit
NGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4330.1492506445697583No Hit
NTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4250.14649312688717617No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3980.13718650470846144No Hit
NCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3970.1368418149981387No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT3840.13236084876394272No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3760.12960333108136055No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3580.12339891629555073No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC3450.11891795006135476No Hit
NCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.11512636324780433No Hit
NTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3340.11512636324780433No Hit
NAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC3220.11099008672393113No Hit
NATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3210.11064539701360836No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3190.10995601759296282No Hit
NTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.10719849991038069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.10202815425553917No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG500.001662003637.7730985
GTCCAAG1709.997428E-635.982371
CGCAGAA2600.034.4983149
GTCTTGG2104.2408057E-529.1285821
GCTATAG650.00598496129.0813373
ACAGTAC650.00601028129.0562278
GTGTAGG1550.0097390826.3096891
TTTGCGT1301.039237E-525.446173
ACTTGAC1301.0462274E-525.4241988
GAGTACT20100.025.13508212-13
CTTTGCG1201.7668458E-423.6285862
GTACTTT21350.023.33174114-15
GTACATG12450.022.9285221
AGTACTT20150.021.67500912-13
CGCTTCG550.00295207121.49353494-95
ATGGCTC1150.00384786220.5288585
ACTTTTT25650.020.52662816-17
TTGGACC1405.009854E-420.2530754
CGATCGT705.0357624E-420.24257924-25
TGTACTG1405.03913E-420.2355885
Produced by FastQC (version 0.11.5)