##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939716_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 290116 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.401298101449076 31.0 31.0 34.0 30.0 34.0 2 31.581484647520302 31.0 31.0 34.0 30.0 34.0 3 31.59888113720029 31.0 31.0 34.0 30.0 34.0 4 35.41815342828386 37.0 35.0 37.0 33.0 37.0 5 35.16436184147031 37.0 35.0 37.0 32.0 37.0 6 35.21365247004646 37.0 35.0 37.0 32.0 37.0 7 35.114195701029935 37.0 35.0 37.0 32.0 37.0 8 35.11101076810655 37.0 35.0 37.0 32.0 37.0 9 36.64061616732617 39.0 35.0 39.0 32.0 39.0 10-11 36.52593273035613 38.0 35.0 39.0 32.0 39.0 12-13 36.47307801017524 38.0 35.0 39.0 32.0 39.0 14-15 37.4064046795075 39.0 36.0 41.0 32.0 41.0 16-17 37.35061664989177 39.0 36.0 41.0 32.0 41.0 18-19 37.334312826593504 39.0 36.0 41.0 32.0 41.0 20-21 37.25162348853562 39.0 36.0 41.0 31.0 41.0 22-23 37.091785699513295 39.0 35.5 40.0 31.0 41.0 24-25 36.896222545464575 39.0 35.0 40.0 31.0 41.0 26-27 36.52770271201864 38.0 35.0 40.0 30.0 41.0 28-29 36.30101580057632 38.0 35.0 40.0 30.0 41.0 30-31 35.9594231273008 38.0 35.0 40.0 30.0 41.0 32-33 35.63045471466586 38.0 34.0 40.0 29.0 41.0 34-35 35.45636917646735 38.0 34.0 40.0 28.5 41.0 36-37 35.260364819589405 38.0 34.0 40.0 27.0 41.0 38-39 35.16590260447545 38.0 34.0 40.0 27.0 41.0 40-41 35.02044009982214 38.0 34.0 40.0 26.0 41.0 42-43 34.73591425498766 38.0 33.0 40.0 25.5 41.0 44-45 34.39484895696894 38.0 33.0 40.0 24.0 41.0 46-47 33.905653255939 37.0 33.0 40.0 23.0 41.0 48-49 33.44822932895807 37.0 33.0 40.0 21.5 41.0 50-51 33.09124281321954 37.0 32.5 40.0 20.0 41.0 52-53 32.85111989686884 36.5 32.0 40.0 18.5 41.0 54-55 32.53831398475093 36.0 31.5 39.5 15.5 41.0 56-57 31.72216802933999 35.5 30.5 39.0 9.5 40.5 58-59 28.832237794537356 34.5 24.0 38.0 2.0 40.0 60-61 28.479683988473575 33.5 24.0 38.0 2.0 40.0 62-63 30.00196128445174 34.5 28.5 38.0 2.0 40.0 64-65 29.96735271408678 35.0 29.0 38.0 2.0 40.0 66-67 25.47557701057508 30.5 16.5 36.0 2.0 38.5 68-69 18.51828406568407 17.5 7.0 31.5 2.0 36.0 70-71 16.614411132098883 12.5 6.5 28.0 2.0 34.5 72-73 18.26732238139227 17.0 7.0 30.5 2.0 35.0 74-75 19.641386548828745 22.0 7.0 31.5 2.0 35.0 76-77 21.296331467413033 26.0 8.0 32.5 2.0 35.0 78-79 23.306274042107297 29.5 9.0 34.0 2.0 35.5 80-81 24.691940465193234 31.0 16.0 35.0 2.0 35.5 82-83 25.619988211611904 32.5 21.0 35.0 2.0 35.0 84-85 25.63132160928732 33.0 20.5 35.0 2.0 35.0 86-87 25.410054943539826 33.0 19.5 35.0 2.0 35.0 88-89 24.979420300845177 32.5 16.5 35.0 2.0 35.0 90-91 23.368523280343034 31.5 2.0 34.5 2.0 35.0 92-93 22.000356753850184 30.5 2.0 34.0 2.0 35.0 94-95 18.626937156172012 25.0 2.0 34.0 2.0 35.0 96-97 16.150610445476982 9.0 2.0 32.5 2.0 35.0 98-99 16.441021867115225 6.5 2.0 32.5 2.0 35.0 100-101 15.749074508127784 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20999130064444316 1101 2 0.11843836659208407 1101 3 0.057152959276852044 1101 4 0.2930498489194733 1101 5 0.46314079468438507 1101 6 0.3368176758453103 1101 7 0.22714529584568055 1101 8 0.2924902954036952 1101 9 0.24884584789262476 1101 10-11 0.12153079959696811 1101 12-13 0.403657282387222 1101 14-15 0.13751493781681035 1101 16-17 -0.023915247592270816 1101 18-19 0.7196933374810612 1101 20-21 0.7771649605496478 1101 22-23 0.6228483235819837 1101 24-25 0.6207386054902884 1101 26-27 0.5551798338265499 1101 28-29 0.28779529531735903 1101 30-31 0.16533150634919735 1101 32-33 0.340693406103739 1101 34-35 0.5366538695058978 1101 36-37 -0.4477872152673186 1101 38-39 -0.02259745262296775 1101 40-41 -0.11176795877270962 1101 42-43 -0.019577671178048206 1101 44-45 -0.1129727344828737 1101 46-47 -0.18881430404451294 1101 48-49 -0.5625417606237235 1101 50-51 -0.32310406881678233 1101 52-53 -0.5363678082166672 1101 54-55 -0.22350921764240894 1101 56-57 -4.004790956610783 1101 58-59 -9.955214813840662 1101 60-61 -11.09759595726236 1101 62-63 -4.495018380902604 1101 64-65 -1.9976879317792111 1101 66-67 -2.6484963111353146 1101 68-69 -4.286554370931977 1101 70-71 -4.948962072220578 1101 72-73 -4.126890010256382 1101 74-75 -5.828250185288184 1101 76-77 -5.050326825198702 1101 78-79 0.12711323544554176 1101 80-81 0.5749620486943208 1101 82-83 -0.8423660998977489 1101 84-85 -0.681674968688629 1101 86-87 -0.650379568843622 1101 88-89 -0.8670308509796918 1101 90-91 0.4768164246958264 1101 92-93 0.379447317614801 1101 94-95 -8.66123434169576 1101 96-97 -9.123831964324543 1101 98-99 -7.964480497807267 1101 100-101 -7.945728089509266 1102 1 0.37021058586372746 1102 2 0.3050411781948945 1102 3 0.2167658688897589 1102 4 0.36535704622667 1102 5 0.5248178563614445 1102 6 0.2293654433930783 1102 7 0.1325383262387092 1102 8 0.49539099830440136 1102 9 0.4600873091340887 1102 10-11 0.30171716728334275 1102 12-13 0.2679033966333364 1102 14-15 0.5527151655170428 1102 16-17 0.27448961331258204 1102 18-19 0.19008612037384864 1102 20-21 0.05977311815779984 1102 22-23 -0.1553605171268586 1102 24-25 0.13576187051354793 1102 26-27 -0.19857598242926144 1102 28-29 0.13131945134151835 1102 30-31 -0.1129268344091372 1102 32-33 0.06578250619283921 1102 34-35 0.2360648189168515 1102 36-37 -0.19502758750768834 1102 38-39 0.1810210009954858 1102 40-41 -0.13456273156749177 1102 42-43 0.14908137248099962 1102 44-45 -0.08101426502440745 1102 46-47 0.07712471189449843 1102 48-49 0.3624966019146356 1102 50-51 0.33175683604412143 1102 52-53 -0.1277572746061395 1102 54-55 -0.4774692216024121 1102 56-57 -3.521874661194488 1102 58-59 -9.714143385269235 1102 60-61 -7.936306233472635 1102 62-63 -2.245897006781231 1102 64-65 -0.48950186109313876 1102 66-67 -1.3682703434093462 1102 68-69 -4.918651440529047 1102 70-71 -3.977505038263544 1102 72-73 -2.9973174431838157 1102 74-75 -3.7547426117806104 1102 76-77 -3.5661977785696557 1102 78-79 1.4263348471671549 1102 80-81 1.6436557423880132 1102 82-83 0.37169067665902844 1102 84-85 0.44135121683755685 1102 86-87 0.20521022424617286 1102 88-89 -0.009943681392520887 1102 90-91 0.764151837031239 1102 92-93 0.24894662461410277 1102 94-95 -7.877462932924349 1102 96-97 -8.481655447148025 1102 98-99 -7.6623445706713404 1102 100-101 -8.660886242167418 1103 1 0.20380844546829735 1103 2 0.3257422483926078 1103 3 0.4023726165770434 1103 4 0.276136113884931 1103 5 0.2717795831889447 1103 6 -0.06559646482386228 1103 7 0.1375492096052966 1103 8 0.3199874067934232 1103 9 0.25135340744716217 1103 10-11 0.18031094562543615 1103 12-13 0.29801404419364275 1103 14-15 0.5830185393606868 1103 16-17 0.590572054814487 1103 18-19 0.4231375442809764 1103 20-21 0.30244951939125286 1103 22-23 0.31138853057856863 1103 24-25 0.3719309254745582 1103 26-27 0.4533716867163946 1103 28-29 0.4790090033095922 1103 30-31 0.8562256234178136 1103 32-33 0.6314228109270985 1103 34-35 0.2695311711751458 1103 36-37 0.820730620364607 1103 38-39 0.8122109157424475 1103 40-41 0.7868049448337473 1103 42-43 0.7264212962974312 1103 44-45 0.9249602497588327 1103 46-47 0.9391326930300039 1103 48-49 0.49104503289595414 1103 50-51 0.42594330439165873 1103 52-53 0.9274077897871109 1103 54-55 0.7332599639589858 1103 56-57 0.710496552300885 1103 58-59 -7.868925264463865 1103 60-61 -7.118818023786439 1103 62-63 -2.0006358059831193 1103 64-65 -0.041957420679572976 1103 66-67 -1.0113979508825928 1103 68-69 -3.2504467425424686 1103 70-71 -3.4748182071371154 1103 72-73 -2.712411312220638 1103 74-75 -3.17279993084464 1103 76-77 -0.2660322607027972 1103 78-79 3.3780965921335984 1103 80-81 2.917803212005282 1103 82-83 2.0113718847127195 1103 84-85 1.4824472625476943 1103 86-87 1.234266545668266 1103 88-89 1.155721564390177 1103 90-91 1.3972690146115063 1103 92-93 0.9415708763826522 1103 94-95 -7.155192844043523 1103 96-97 -8.20741728280919 1103 98-99 -7.861940979160366 1103 100-101 -8.974860031392886 1104 1 0.3912267318798719 1104 2 0.4057344038881219 1104 3 0.3267167288406192 1104 4 0.2824770883467096 1104 5 0.08185541339900482 1104 6 0.1014696904973249 1104 7 0.15188722058760362 1104 8 0.3764896294030322 1104 9 0.5438008928476705 1104 10-11 0.34530702337319497 1104 12-13 0.26088338961332624 1104 14-15 0.48187890718078563 1104 16-17 0.4876565139794877 1104 18-19 0.5186443864321149 1104 20-21 0.40524521362990384 1104 22-23 0.4616761624098231 1104 24-25 0.5100159947676772 1104 26-27 0.6559124345591556 1104 28-29 0.27147827900034116 1104 30-31 0.7680621090797999 1104 32-33 0.7179411583514863 1104 34-35 0.8654084482604745 1104 36-37 0.6821661646860591 1104 38-39 0.6844871294616155 1104 40-41 1.0031171561124026 1104 42-43 0.940587163986784 1104 44-45 0.8043183078081668 1104 46-47 0.9205346803044705 1104 48-49 1.1428361947542243 1104 50-51 1.2924886717759563 1104 52-53 0.94735680050794 1104 54-55 0.7572183880851924 1104 56-57 1.317345240525416 1104 58-59 -7.710084943710793 1104 60-61 -6.953614938281341 1104 62-63 -1.0542508151350383 1104 64-65 1.0631262540349766 1104 66-67 -1.0140890517280567 1104 68-69 -3.5525800119576196 1104 70-71 -2.9846347328932374 1104 72-73 -1.848251981618354 1104 74-75 -2.3147636718016713 1104 76-77 0.8191786693067904 1104 78-79 4.0911075744398815 1104 80-81 3.850394949127221 1104 82-83 2.536463403931755 1104 84-85 2.217259430245768 1104 86-87 2.5946670512030003 1104 88-89 2.4680091090602687 1104 90-91 3.9476372705166725 1104 92-93 3.000284813452293 1104 94-95 -4.395583326044743 1104 96-97 -6.814974155466734 1104 98-99 -5.2220146303414 1104 100-101 -4.694604213385389 1105 1 0.1043444722253355 1105 2 0.24428611620220764 1105 3 0.3259666538331203 1105 4 0.28760974446946364 1105 5 0.11254419656303583 1105 6 0.16287940289713987 1105 7 0.2091929511606594 1105 8 0.2628961271956669 1105 9 0.3026303472216796 1105 10-11 0.24766868811109077 1105 12-13 0.07699714384589385 1105 14-15 0.5118122576586828 1105 16-17 0.6488262023768954 1105 18-19 0.6218439921069674 1105 20-21 0.20535558180957736 1105 22-23 0.4859571619383516 1105 24-25 0.2317167362703998 1105 26-27 0.27165972357376944 1105 28-29 0.5236963579511027 1105 30-31 0.7025055534242313 1105 32-33 0.5541087036774499 1105 34-35 0.8570397542482198 1105 36-37 0.7156516560923407 1105 38-39 0.9296384303059853 1105 40-41 1.323313461457225 1105 42-43 1.1281755656841028 1105 44-45 1.344666985533074 1105 46-47 1.3182940991034968 1105 48-49 1.2224834451935607 1105 50-51 1.175771642930215 1105 52-53 1.2981079470511645 1105 54-55 1.5944467537789038 1105 56-57 1.820908096811042 1105 58-59 -8.208161537084418 1105 60-61 -6.176767610691439 1105 62-63 -0.8290407941329399 1105 64-65 0.9074910476571958 1105 66-67 0.04168974043687612 1105 68-69 -2.836349460331027 1105 70-71 -3.3659460783134953 1105 72-73 -2.27567686696403 1105 74-75 -2.622015825588477 1105 76-77 0.8911376509192372 1105 78-79 4.6398249461770575 1105 80-81 4.268031355625013 1105 82-83 3.102507508342196 1105 84-85 2.392219783423947 1105 86-87 2.283777033629814 1105 88-89 2.2855640788429596 1105 90-91 3.3519116265237017 1105 92-93 2.4276829104048474 1105 94-95 -5.322751042816421 1105 96-97 -6.842941237889262 1105 98-99 -4.552374452037856 1105 100-101 -4.097271265804919 1106 1 0.023311534494478536 1106 2 0.13122249195899016 1106 3 0.23972094316933834 1106 4 0.06150435247331387 1106 5 0.07045952451966997 1106 6 0.0018954237045178957 1106 7 0.16392815978085906 1106 8 0.22449427376528774 1106 9 -0.0319925190384609 1106 10-11 0.07311729257419586 1106 12-13 0.019290545371482892 1106 14-15 -0.18208213178025545 1106 16-17 0.1605084964341188 1106 18-19 0.03892046796647719 1106 20-21 0.13743107412702216 1106 22-23 -0.08160671365450867 1106 24-25 0.07495105970274096 1106 26-27 -0.24049248502456777 1106 28-29 -0.04969227230265716 1106 30-31 -0.01053512150419067 1106 32-33 0.18890314338631953 1106 34-35 -0.06056127737812034 1106 36-37 0.24400498811429117 1106 38-39 -0.27499295998536155 1106 40-41 -0.2911762987770814 1106 42-43 -0.6583958066849291 1106 44-45 -0.3911426881495217 1106 46-47 -0.3363351055388222 1106 48-49 -0.4324626785578829 1106 50-51 -0.6741944795098433 1106 52-53 -0.682997117097635 1106 54-55 -0.6235225597087322 1106 56-57 -0.6005506053505663 1106 58-59 -8.935583782620231 1106 60-61 -7.605728553143301 1106 62-63 -2.423832392498074 1106 64-65 -1.028890173246353 1106 66-67 0.2825373351301188 1106 68-69 -2.2700587252972575 1106 70-71 -2.2708758096972286 1106 72-73 -1.6489529362928188 1106 74-75 -1.5386606357648596 1106 76-77 1.247868357102437 1106 78-79 3.487179218028082 1106 80-81 2.65388037099013 1106 82-83 1.3381641865928025 1106 84-85 0.4454567296351222 1106 86-87 0.5180458247473361 1106 88-89 0.5334494634079086 1106 90-91 1.9694722236430167 1106 92-93 1.5178409328296034 1106 94-95 -5.543987901601636 1106 96-97 -6.5622219407939895 1106 98-99 -4.380686256728259 1106 100-101 -3.9959609692123443 1107 1 -0.0439614976121554 1107 2 0.19329810600878616 1107 3 0.07470291986478728 1107 4 -0.07729604990743866 1107 5 -6.696564678421169E-4 1107 6 -0.07438926067808183 1107 7 -0.010080558468786194 1107 8 0.10709130620723784 1107 9 0.01063469195995026 1107 10-11 0.177380013674032 1107 12-13 0.3066050729932357 1107 14-15 0.07899381758682722 1107 16-17 0.2267860553242187 1107 18-19 0.3451280293714518 1107 20-21 0.5634936108023467 1107 22-23 0.4266399960445497 1107 24-25 0.24741941413311963 1107 26-27 0.13624697343799852 1107 28-29 0.10794877053412932 1107 30-31 0.048700774212136366 1107 32-33 0.23068158248431558 1107 34-35 -0.019537302191601214 1107 36-37 0.23997070192097425 1107 38-39 0.37230564006492983 1107 40-41 -0.31280426423939645 1107 42-43 -0.33992820671846147 1107 44-45 -0.5737229547267688 1107 46-47 -0.8286228077770659 1107 48-49 -0.42840743503370504 1107 50-51 -0.5157884940961495 1107 52-53 -0.3464538192520479 1107 54-55 -0.6721750033335141 1107 56-57 -0.1564752427887406 1107 58-59 -7.888985157421136 1107 60-61 -7.138474382634456 1107 62-63 -2.469383151153451 1107 64-65 -1.284810619218355 1107 66-67 0.403817408899922 1107 68-69 -3.197923592427781 1107 70-71 -3.1673570738434265 1107 72-73 -1.445038106885491 1107 74-75 -1.220916063713556 1107 76-77 1.6980213456178213 1107 78-79 3.9302905433696864 1107 80-81 2.584983145740736 1107 82-83 0.7411527019754836 1107 84-85 0.7466240529648225 1107 86-87 0.5630831287962934 1107 88-89 0.9336716111151766 1107 90-91 2.293231549655257 1107 92-93 2.339200644456735 1107 94-95 -5.931688044111485 1107 96-97 -7.4350423783830575 1107 98-99 -4.824677789966584 1107 100-101 -4.132854254483531 1108 1 -0.0967852561321152 1108 2 -0.020755772602054634 1108 3 -0.0861620590381662 1108 4 -0.18817725730763613 1108 5 -0.17788484634125723 1108 6 -0.075728111700478 1108 7 0.007365076065461551 1108 8 -0.0500005470871443 1108 9 -0.00985378580701024 1108 10-11 0.1849465505127199 1108 12-13 -0.10137934764941292 1108 14-15 0.01503325283513135 1108 16-17 -0.15005993623695701 1108 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66-67 -2.253916884445797 2313 68-69 -4.515172847256876 2313 70-71 -3.4655464053186726 2313 72-73 -2.3230623394562357 2313 74-75 -2.984885598804331 2313 76-77 0.2762013866506088 2313 78-79 2.572168180500487 2313 80-81 1.6330660242294925 2313 82-83 0.22929771029817303 2313 84-85 0.2244479603700782 2313 86-87 -0.13665170649190728 2313 88-89 -0.1262807753672277 2313 90-91 1.6865317857873343 2313 92-93 2.680082462286638 2313 94-95 4.790474499921341 2313 96-97 1.2009319567696526 2313 98-99 3.113081084341472 2313 100-101 4.4140528023817165 2314 1 -0.4118740135285641 2314 2 -0.4152330901562955 2314 3 -0.4148109742255457 2314 4 -0.233295600502899 2314 5 -0.5713759706016148 2314 6 -0.36788787501408393 2314 7 -0.37231934208049466 2314 8 -0.3024660303218596 2314 9 -0.5968853439924118 2314 10-11 -0.5792299780868859 2314 12-13 -0.26632017605177793 2314 14-15 -0.5992418950938614 2314 16-17 -0.5345520466906066 2314 18-19 -0.5748966878781943 2314 20-21 -0.30363244717083404 2314 22-23 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2314 88-89 0.2525803070930053 2314 90-91 1.7805639592125893 2314 92-93 3.4129778896069105 2314 94-95 5.079537706768594 2314 96-97 1.887227489042603 2314 98-99 4.128855653221189 2314 100-101 4.975658216492425 2315 1 -0.1826369044837648 2315 2 -0.4110946004408831 2315 3 -0.41056682094292896 2315 4 -0.09522853935891362 2315 5 -0.33264675110316233 2315 6 -0.5592445952169598 2315 7 -0.5523876711872902 2315 8 -0.5831965177831151 2315 9 -0.6501926136458351 2315 10-11 -0.5854289073627399 2315 12-13 -0.5982886883087488 2315 14-15 -0.8799987984469197 2315 16-17 -0.5979766029036284 2315 18-19 -0.6909064793687492 2315 20-21 -0.69662166948698 2315 22-23 -0.7005262185425565 2315 24-25 -0.38784655934487944 2315 26-27 -0.6000399463932311 2315 28-29 -0.9215682577961886 2315 30-31 -1.0127820467643573 2315 32-33 -1.048157326496998 2315 34-35 -0.7521510755490439 2315 36-37 -0.6268165192966251 2315 38-39 -1.100550749326267 2315 40-41 -0.8939337096884614 2315 42-43 -0.7647699788703548 2315 44-45 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-0.6261607084102252 2316 6 -0.30903934070136074 2316 7 -0.44267913259943725 2316 8 -0.5477901138422325 2316 9 -0.6786203640563429 2316 10-11 -0.6886719287092049 2316 12-13 -0.6737710364204119 2316 14-15 -0.6691510972974939 2316 16-17 -1.0272151086507506 2316 18-19 -0.7174356255099639 2316 20-21 -0.4797189601273786 2316 22-23 -0.7031432136164497 2316 24-25 -0.5328075609869103 2316 26-27 -0.5626836245541469 2316 28-29 -0.8632246617370782 2316 30-31 -0.5928313078481011 2316 32-33 -0.5259385251833706 2316 34-35 -0.8722762808035611 2316 36-37 -0.755100591546217 2316 38-39 -0.9512794028566418 2316 40-41 -0.7372732006572633 2316 42-43 -0.6649136570312706 2316 44-45 -0.2942598381837698 2316 46-47 -0.35868609891630854 2316 48-49 -0.528327411754205 2316 50-51 -0.6186547824709479 2316 52-53 -0.48574888690809814 2316 54-55 -0.6104297683560667 2316 56-57 0.06839017260482905 2316 58-59 2.7161727066847874 2316 60-61 2.6682875844832523 2316 62-63 0.4589414523675721 2316 64-65 0.08973470305721776 2316 66-67 -1.6166976500263104 2316 68-69 -2.2440824750118047 2316 70-71 -2.499580993529154 2316 72-73 -0.8609591020840952 2316 74-75 5.030866272966204 2316 76-77 5.174268981293654 2316 78-79 4.382513008086697 2316 80-81 3.1827206693839756 2316 82-83 1.5807424007969573 2316 84-85 0.8951217977633092 2316 86-87 0.5058664839541542 2316 88-89 0.7474538022980681 2316 90-91 1.8256227907435694 2316 92-93 3.363753241575896 2316 94-95 4.96348145974418 2316 96-97 1.9976471312924833 2316 98-99 4.794629644923566 2316 100-101 4.79983525519201 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 14.0 5 53.0 6 134.0 7 213.0 8 343.0 9 581.0 10 910.0 11 1344.0 12 1652.0 13 2289.0 14 2776.0 15 3657.0 16 4574.0 17 5688.0 18 5863.0 19 5144.0 20 5032.0 21 5333.0 22 5356.0 23 5397.0 24 5497.0 25 5927.0 26 6935.0 27 9704.0 28 13154.0 29 16621.0 30 19631.0 31 21475.0 32 22218.0 33 23170.0 34 24069.0 35 24241.0 36 21689.0 37 14484.0 38 4717.0 39 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78706385326162 19.992070059302165 16.00917114880706 20.211694938629154 2 16.36733427556965 23.51028991002792 36.493846737219485 23.628529077182943 3 20.615546884694396 26.07991286244123 31.97583035751217 21.328709895352205 4 12.85106646996374 18.813853768837294 36.607081305408876 31.727998455790097 5 12.523669424794177 38.392238153215764 33.90668814304093 15.17740427894913 6 28.89430434722663 36.89110562671483 16.969763818610488 17.244826207448057 7 24.46435219015842 35.690896055370956 21.618593941733653 18.22615781273697 8 25.30720576323182 31.59505713251642 20.646984816365922 22.450752287885837 9 24.466075638710034 18.193756979966633 22.4717009747825 34.86846640654083 10-11 24.75509796081567 26.266217650870686 26.64744447048767 22.331239917825975 12-13 27.271160501316714 24.73700175102373 27.25065146355251 20.741186284107048 14-15 23.057580614583873 26.333867604225908 28.37150785033521 22.237043930855005 16-17 20.34393139296006 29.421334914310137 28.53220780653256 21.702525886197247 18-19 19.99672543646485 28.326353342639987 30.93204418937318 20.744877031521984 20-21 21.94242992458189 26.221752678239053 30.12639771677536 21.7094196804037 22-23 20.564360462711466 26.56075500834149 31.423637441575092 21.451247087371947 24-25 22.273159701636587 26.938879620565565 30.45126776875457 20.33669290904328 26-27 21.60553296876589 25.76353211048703 32.67043642962889 19.96049849111819 28-29 20.1646941628018 28.35304113734797 31.383062146366854 20.099202553483376 30-31 20.730996873459567 26.833955539009363 31.153028125075405 21.28201946245566 32-33 21.131008807459626 27.095778968958445 31.421258553232562 20.351953670349367 34-35 21.997383746481933 26.56565882882153 30.944867369045138 20.492090055651403 36-37 21.21432698858981 26.66568922543532 31.786561332999852 20.333422452975014 38-39 20.95714295570105 26.926566262150992 31.41096400753379 20.705326774614168 40-41 20.69157037565998 27.32249768435868 31.183279776042554 20.80265216393878 42-43 20.780464076504316 27.72056743460772 29.68785576783755 21.811112721050417 44-45 21.053866309708425 26.58815655332897 30.076434373560712 22.28154276340189 46-47 22.255378676413514 27.727803526735123 29.615019068794275 20.401798728057088 48-49 21.80272818683716 27.0464419566091 29.793083465131883 21.357746391421855 50-51 19.651585130711627 28.027028798294662 30.8813333655269 21.44005270546681 52-53 21.199663387917834 28.555120228454754 28.71342447611296 21.53179190751445 54-55 20.242709741495847 27.239150826275278 29.51778767898033 23.000351753248545 56-57 21.35804789884228 27.418094773276398 28.791560532347326 22.432296795533993 58-59 21.339651977150158 27.172366402676456 30.035364507777416 21.452617112395973 60-61 20.61064346334479 27.812342212108426 30.901872358673927 20.675141965872857 62-63 20.09437848253326 28.06105301981845 29.947588574619495 21.8969799230288 64-65 19.894522767226224 29.09879011409465 29.253731343283583 21.752955775395538 66-67 20.88885869470545 27.370040169877118 28.716062028143302 23.02503910727413 68-69 20.335631324179005 27.22695776118771 30.113500049393867 22.32391086523942 70-71 21.67458400879169 27.130405945327123 29.069022877090983 22.125987168790207 72-73 21.166714035586338 26.55852517745801 30.1625982027881 22.112162584167553 74-75 20.01392631270331 28.710018568416938 29.07999078559761 22.19606433328214 76-77 19.255434763738382 27.76477655056995 29.287829809692123 23.691958875999543 78-79 20.681144600239183 27.65689723902456 29.05835658699759 22.60360157373867 80-81 21.35256288684678 28.008999844830264 28.178479681384804 22.459957586938156 82-83 20.8105406476742 27.395860262309775 28.994881340158212 22.798717749857815 84-85 20.658127406406514 27.540148425947308 28.486682683524585 23.315041484121597 86-87 20.384920970040827 28.28929566735548 28.10316033662919 23.222623025974496 88-89 20.17882502171545 28.14856815894332 27.435577493140674 24.237029326200556 90-91 20.75241039686821 26.934023599064886 28.140259506715914 24.173306497350985 92-93 20.87001205332432 27.831970796122935 28.284961969089 23.013055181463745 94-95 19.916852935804553 27.572297068109275 28.90317193873557 23.6076780573506 96-97 18.885179398431973 27.775578552060924 29.12903655229914 24.210205497207955 98-99 19.787238839490886 27.88406008230881 27.915103649093233 24.413597429107075 100-101 18.815637152621324 27.09259041030369 28.982073908733597 25.109698528341383 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2966.0 1 2056.5 2 1047.5 3 924.5 4 853.5 5 873.5 6 1064.0 7 1473.0 8 1790.5 9 1781.0 10 1692.5 11 1549.0 12 1428.0 13 1321.0 14 1234.0 15 1184.5 16 1219.0 17 1300.5 18 1252.5 19 1205.5 20 1238.0 21 1270.5 22 1321.0 23 1425.0 24 1463.5 25 1554.0 26 1698.0 27 1907.5 28 2228.5 29 2530.5 30 2763.0 31 2937.5 32 3095.5 33 3339.0 34 3652.5 35 3909.0 36 4163.5 37 4437.0 38 4773.5 39 5148.0 40 5467.0 41 5810.5 42 6252.0 43 6740.0 44 7411.0 45 8720.0 46 10649.0 47 13371.0 48 15773.0 49 15613.0 50 13555.0 51 11507.5 52 10171.5 53 9560.0 54 9090.0 55 8496.0 56 7806.5 57 7021.0 58 6273.0 59 5581.0 60 4840.5 61 4039.5 62 3368.5 63 2789.0 64 2278.5 65 1763.0 66 1270.5 67 955.5 68 674.5 69 418.0 70 305.0 71 235.5 72 189.0 73 150.0 74 109.0 75 84.0 76 57.5 77 40.0 78 32.5 79 18.5 80 12.0 81 10.0 82 5.0 83 4.0 84 2.5 85 2.5 86 2.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026196417984530325 2 0.008961932468391953 3 0.0 4 0.0 5 0.06376759640971197 6 0.0 7 0.0 8 3.446897103227675E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.446897103227675E-4 16-17 0.0 18-19 3.446897103227675E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7234485516138374E-4 28-29 8.617242758069187E-4 30-31 0.006549104496132581 32-33 0.0072384839167781165 34-35 0.002240483117097988 36-37 0.08393194446359387 38-39 0.07652111569165437 40-41 0.08255318562230281 42-43 0.08617242758069187 44-45 0.08944697982875816 46-47 0.08427663417391663 48-49 0.07462532228487916 50-51 0.0699720111955218 52-53 0.05790787133422493 54-55 0.04825655944518744 56-57 0.06635276923713272 58-59 0.6804174881771429 60-61 0.3315915013305023 62-63 0.0025851728274207558 64-65 0.002068138261936605 66-67 1.0633677563457375 68-69 2.302527264956087 70-71 2.297873953866729 72-73 1.7644666271422464 74-75 1.2436404748445449 76-77 0.7269505990707166 78-79 0.5621889175364337 80-81 0.03826055784582719 82-83 3.446897103227675E-4 84-85 0.0017234485516138373 86-87 0.0015511036964524535 88-89 0.0 90-91 0.3281446042272746 92-93 0.05325456024486758 94-95 0.9215279405479189 96-97 1.6069434295247418 98-99 0.6244054102496932 100-101 0.5508141570957824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 290116.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.94866054967671 #Duplication Level Percentage of deduplicated Percentage of total 1 71.77207320288605 30.10742335734415 2 13.428109675566438 11.26582429208332 3 5.58076410720068 7.023167374223426 4 2.7674214102468064 4.643584853454036 5 1.6193021212341188 3.3963777505510744 6 1.0356345880550275 2.6066090272694775 7 0.6993705307894251 2.0536359896172973 8 0.4856813984729727 1.6298947295868 9 0.4059641447687766 1.5326686893820698 >10 2.0191329778713047 14.74854163944594 >50 0.1150502995910825 3.2521733615288295 >100 0.04930727125332107 3.9278421964447197 >500 0.010683242104886232 3.0935980227220394 >1k 0.011505029959108251 10.718658716346821 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4029 1.38875484289043 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3928 1.3539411821478304 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3733 1.2867266886348907 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3374 1.1629830826290175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2739 0.94410511657406 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2500 0.8617242758069186 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 0.7235037019674889 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1750 0.603206993064843 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1377 0.4746377311144508 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1262 0.43499841442733256 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1131 0.38984406237505 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1093 0.3767458533827848 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1071 0.36916267975568395 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1007 0.34710253829502685 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 930 0.3205614306001737 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 919 0.3167698437866233 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 775 0.26713452550014477 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 751 0.2588619724523984 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 748 0.25782790332143 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 688 0.237146520702064 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 687 0.23680183099174124 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 668 0.23025272649560866 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 645 0.222324863158185 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 565 0.19474968633236361 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 558 0.19233685836010425 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 523 0.18027271849880738 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 517 0.17820458023687077 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 477 0.16441699182396008 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 467 0.1609700947207324 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 415 0.1430462297839485 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 414 0.1427015400736257 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 409 0.14097809152201188 TruSeq Adapter, Index 3 (95% over 21bp) ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 356 0.1227095368749052 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 354 0.12202015745425969 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 328 0.11305822498586772 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 291 0.10030470570392533 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.446897103227674E-4 0.0 12-13 0.0 0.0 0.0 6.893794206455348E-4 0.0 14-15 0.0 0.0 0.0 8.617242758069186E-4 0.0 16-17 0.0 0.0 0.0 0.001206413986129686 0.0 18-19 0.0 0.0 0.0 0.0015511036964524535 0.0 20-21 0.0 0.0 0.0 0.0027575176825821394 0.0 22-23 0.0 0.0 0.0 0.004480966234195976 0.0 24-25 0.0 0.0 0.0 0.007583173627100884 0.0 26-27 0.0 0.0 0.0 0.01344289870258793 0.0 28-29 0.0 0.0 0.0 0.038605247556149955 0.0 30-31 0.0 0.0 0.0 0.08031270250520481 0.0 32-33 0.0 0.0 0.0 0.15166347254201767 0.0 34-35 0.0 0.0 0.0 0.25386397165271823 0.0 36-37 0.0 0.0 0.0 0.376401163672462 0.0 38-39 0.0 0.0 0.0 0.5301327744764164 0.0 40-41 0.0 0.0 0.0 0.6997201119552179 0.0 42-43 0.0 0.0 0.0 0.8570709647175613 0.0 44-45 0.0 0.0 0.0 1.042858718581533 0.0 46-47 0.0 0.0 0.0 1.2529470970232597 0.0 48-49 0.0 0.0 0.0 1.430979332404969 0.0 50-51 0.0 0.0 0.0 1.631761088667981 0.0 52-53 0.0 0.0 0.0 1.8197893256490507 0.0 54-55 0.0 0.0 0.0 2.01402197741593 0.0 56-57 0.0 0.0 0.0 2.195328765045706 0.0 58-59 0.0 0.0 0.0 2.322002233589323 0.0 60-61 0.0 0.0 0.0 2.4381626659680955 0.0 62-63 0.0 0.0 0.0 2.5401908202236347 0.0 64-65 0.0 6.893794206455348E-4 0.0 2.6537660797749867 0.0 66-67 0.0 6.893794206455348E-4 0.0 2.7538984406237503 0.0 68-69 0.0 6.893794206455348E-4 0.0 2.8647161824925202 0.0 70-71 0.0 6.893794206455348E-4 0.0 3.018447793296475 0.0 72-73 0.0 6.893794206455348E-4 0.0 3.244219553557887 0.0 74-75 0.0 6.893794206455348E-4 0.0 3.5799473314122627 0.0 76-77 0.0 6.893794206455348E-4 0.0 3.9546250465331108 0.0 78-79 0.0 6.893794206455348E-4 0.0 4.443567400625956 0.0 80-81 0.0 6.893794206455348E-4 0.0 5.012822457224007 0.0 82-83 0.0 6.893794206455348E-4 0.0 5.543127576555585 0.0 84-85 0.0 6.893794206455348E-4 0.0 6.053440692688442 0.0 86-87 0.0 6.893794206455348E-4 0.0 6.5351445628645095 0.0 88-89 0.0 6.893794206455348E-4 0.0 6.95859587199603 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 215 0.0 41.665188 9 TTCGGTA 30 0.0035286592 32.4034 64-65 GCATAGG 30 0.0035286592 32.4034 66-67 GCTTACG 60 1.4069337E-7 31.907328 60-61 GCGGTAT 100 5.1674026E-5 28.312868 1 AACCTAC 35 0.007491146 27.779276 68-69 AATAGAG 70 0.008676945 26.950687 5 CGCATAT 55 8.0973565E-5 26.511873 66-67 TACTCGG 65 8.501049E-6 26.120924 62-63 CAACGTA 50 0.0016644819 23.720907 86-87 AGACCCG 120 1.7907315E-4 23.581854 5 GTACATA 165 2.5619884E-6 22.879086 1 CTTTGCG 165 2.5735371E-6 22.867252 2 TTTGCGT 165 2.5735371E-6 22.867252 3 GTGTATG 105 0.0022778371 22.47053 1 GAAGAGT 190 3.5127778E-7 22.333002 6 ACGCATA 175 0.0 22.219473 64-65 GAGTACT 2175 0.0 22.002142 12-13 AAACCGT 65 3.0801084E-4 21.77162 40-41 GTAGGCT 110 0.0029931115 21.438047 4 >>END_MODULE